JPS61202680A - Culture device - Google Patents

Abstract

PURPOSE:To enable high-efficiency cultivation in carrier process, by using a medium-preparation chamber having a medium-feeding port, an aeration gas feeding pipe and a stirring member and separating the preparation chamber from the upper culture chamber with a perforated plate having a pore size to inhibit the passage of the carrier. CONSTITUTION:Microorganisms or cells are attached to a carrier, and are cultured in a culture tank 1 in floating state. The medium-preparation chamber A of the lower part of the culture tank 1 is furnished with the medium-feeding port 11, the aeration gas feeding pipe 10 and the stirring member 6, and the microorganisms or cells attached to the carrier are cultured in the culture chamber above the medium-preparation chamber A. The medium-preparation chamber A is separated from the culture chamber with a perforated plate 8 having a pore size to inhibit the passage of the carrier. An inverted conical separation chamber C having larger diameter at the upper part is placed above the culture chamber, and the medium-extraction port 13 to extract the medium in the separation chamber is attached to the top of the culture tank 1. A high- efficiency cultivation by a carrier process can be realized by the above apparatus.

Description

Translated fromJapanese

【発明の詳細な説明】〔発明の利用分野〕本発明は、担体に微生物あるいは細胞を付着させ培養槽
内で浮遊培養させる培養装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to a culture device for attaching microorganisms or cells to a carrier and culturing them in suspension in a culture tank.

〔発明の背景〕[Background of the invention]

微生物または細胞を培養する培養槽Ｉζよる培養槽によ
る培養装置において、培養の容積効率を高めるために、
培地への通気ガス供給を増大させることがある。この場
合、培地の激しい撹拌が行なわれるのが普通である。In a culture device using a culture tank Iζ for culturing microorganisms or cells, in order to increase the volumetric efficiency of culture,
Aeration gas supply to the culture medium may be increased. In this case, the medium is usually vigorously stirred.

一方、担体に微生物あるいは細胞を付着させこれを浮遊
させながら微生物あるいは細胞を培養する。いわゆる担
体法と呼ばれる培養方法が知られているが、この方法Ｉ
こよって培養される微生物あるいは細胞は元々物理的な
衝撃Ｉこよって破壊されやすい性質を有するものが多い
。したがって、担体法ｌこよる培養装置において、上述
した如き激しい撹拌を行うと、微生物や細胞が担体から
はく離したり破壊される可能性が大きく、ゆるやかな撹
拌しかできない。これは、培養の容積効率を高める上で
の大きな障害となる。On the other hand, microorganisms or cells are attached to a carrier and cultured while the microorganisms or cells are suspended. A culture method called the so-called carrier method is known, but this method I
Many of the microorganisms or cells that are cultured in this way have the property of being easily destroyed by physical shock. Therefore, in a culture apparatus based on the carrier method, if vigorous stirring as described above is performed, there is a high possibility that the microorganisms and cells will be detached from the carrier or destroyed, and only gentle stirring is possible. This becomes a major obstacle in increasing the volumetric efficiency of culture.

一般に、高収率培養を行なうためには、激しい撹拌によ
る高く均一な溶存ガス濃度の実現、担体や細胞等を破壊
しないゆるやかな撹拌が必要となる。Generally, in order to perform high-yield culture, it is necessary to achieve a high and uniform dissolved gas concentration through vigorous stirring, and to perform gentle stirring that does not destroy carriers, cells, etc.

な自、担体法による培養方法は、例えば、特公昭５６−
１４２７０号、特公昭５８−２５６４５号等に開示され
ている。The culture method using the carrier method is, for example, disclosed in Japanese Patent Publication No. 1983-
14270, Japanese Patent Publication No. 58-25645, etc.

〔発明の目的〕[Purpose of the invention]

本発明の目的は、担体法による高効率培養を行なうに適
した培養装置を提供することである。An object of the present invention is to provide a culture device suitable for performing highly efficient culture using a carrier method.

〔発明の概要〕[Summary of the invention]

本発明は、担体法による培養装置において、培養槽下部
に培地供給口、通気ガス供給部、および撹拌部を有する
培地調整室を設け、該培養槽内の該培地調整室上部Ｉこ
担体に付着された微生物あるいは細胞を培養する培養室
を設け、該培地調整室と該培養室との間は担体を通過さ
せない程度の孔寸法を有する多孔板で仕切り、該培養室
の上部番こは上部にいくほど径が大なる逆円錐状の分離
室を備え、該分離室内の培地を抜出す培地抜出部を該培
養槽上部に設けたことを特徴とする。The present invention provides a culture apparatus using a carrier method, in which a culture medium adjustment chamber having a medium supply port, an aeration gas supply part, and a stirring part is provided at the bottom of the culture tank, and the upper part of the medium adjustment chamber in the culture tank is attached to the carrier. A culture chamber is provided for culturing the microorganisms or cells, and the culture medium adjustment chamber and the culture chamber are partitioned with a perforated plate having a hole size that does not allow the carrier to pass through. The present invention is characterized in that it includes an inverted conical separation chamber whose diameter increases as the diameter increases, and that a medium extraction part for extracting the medium inside the separation chamber is provided at the top of the culture tank.

〔発明の実施例〕以下、本発明を第１図に示す具体的な実施例を用いて詳
細に説明する。[Embodiments of the Invention] Hereinafter, the present invention will be explained in detail using a specific embodiment shown in FIG.

第１図において、１は培養槽を示し、培地調整室Ａ、培
培養室８升を有する。培地調整室Ａと培養室Ｂとの間は、担体を通
過させない程度の孔寸法を有する多孔板８で仕切られて
いる。２はジャケットであり、この内部には培養槽の温
度を調整する温度調節部（図示せず）が設けられる。３
は培養槽の分離室Ｃを形成する逆円離調であり、上部に
行くほど径が大となる逆円錐状となっている。４は培地
抜出部りを形成するもので、培養槽頂部から分離室Ｃ内
の培地に届く長さを有する円錐管である。５はバッフル
、６は撹拌翼、７は撹拌翼駆動装置である。In FIG. 1, 1 indicates a culture tank, which has a culture medium adjustment chamber A and a culture chamber of 8 sq. The culture medium adjustment chamber A and the culture chamber B are partitioned by a perforated plate 8 having a hole size that does not allow the carrier to pass through. Reference numeral 2 denotes a jacket, and a temperature control unit (not shown) for adjusting the temperature of the culture tank is provided inside the jacket. 3
is an inverted circular detuning forming the separation chamber C of the culture tank, and has an inverted conical shape whose diameter increases toward the top. 4 forms a medium extraction part, and is a conical tube having a length that reaches the medium in the separation chamber C from the top of the culture tank. 5 is a baffle, 6 is a stirring blade, and 7 is a stirring blade driving device.

９は温度．ＰＨ，溶存酸素等を検出するセンサ群である
。１０はスパージャであり、ここから通気ガスを供給す
る。１１は培地供給口であり、ここから培地が供給され
る。培地調整室Ａ内には、撹拌手段である撹拌翼６，バ
ッフル５１通気ガス供給部であるスパージャｌＯ１培地
供給口１１が設けられている。１２はオーバレイガス入
口、１３は培地抜出口、１４は排気ガス出口である。１
５は除菌フィルターを示す。9 is temperature. This is a group of sensors that detect pH, dissolved oxygen, etc. 10 is a sparger from which ventilation gas is supplied. Reference numeral 11 denotes a medium supply port, from which the medium is supplied. Inside the culture medium adjustment chamber A, there are provided a stirring blade 6 as a stirring means, a baffle 51, a sparger lO1 as a ventilation gas supply part, and a culture medium supply port 11. 12 is an overlay gas inlet, 13 is a medium outlet, and 14 is an exhaust gas outlet. 1
5 indicates a sterilization filter.

さて、上述した如き第１回の培養装置で）よ、培地は培
地供給口１１より培地調整室Ａ内に供給される。また、
通気ガスは、スパージャ１０より培地調整室Ａ内に供給
される。調整室Ａ内におｔｌて、培地と通気ガスは、バ
ッフル５，撹拌翼６；こより激しく撹拌される。また、
センサ一群９は、培地の状態を検出し、温度，ＰＨ，溶
存酸素等が最適になるよう図示しない各調整手段を調整
する。具体的には、ＰＨ，溶存酸素については、供給す
る通気ガスの成分を調節するなどの方法が採られる。Now, in the first culture apparatus as described above), the medium is supplied into the medium adjustment chamber A from the medium supply port 11. Also,
Venting gas is supplied into the culture medium adjustment chamber A from the sparger 10. Inside the adjustment chamber A, the culture medium and ventilation gas are vigorously stirred by baffles 5 and stirring blades 6. Also,
The sensor group 9 detects the state of the culture medium and adjusts each adjustment means (not shown) so that the temperature, pH, dissolved oxygen, etc. are optimized. Specifically, with regard to pH and dissolved oxygen, methods such as adjusting the components of the ventilation gas to be supplied are adopted.

また温度は、ジャケット２内の温度調節器を調節するな
どの方法が採られる。培地調整室Ａで高（均一な溶存ガ
ス濃度に調整された培地は、撹拌の作用により多孔板８
を通過して培養室Ｂに供給される。培養室Ｂ内では、担
体法による浮遊培養が行なわれる。この例では、培地調
整室Ａに詔ける激しい撹拌を多孔板８でゆるやかなもの
にして培養室Ｂ内での培地の撹拌を行なっている。この
ようなゆるやかな撹拌ｌこよって、培養室Ｂにおいて微
生物あるいは細胞等が破壊されることはない。Further, the temperature can be adjusted by using a temperature controller inside the jacket 2, or the like. The medium, which has been adjusted to a high (uniform dissolved gas concentration) in the medium adjustment chamber A, is transferred to the perforated plate 8 by the action of stirring.
It passes through and is supplied to culture room B. In culture chamber B, suspension culture is performed using a carrier method. In this example, the vigorous stirring in the culture medium adjustment chamber A is made gentle using the perforated plate 8, and the culture medium in the culture chamber B is stirred. Due to such gentle stirring, microorganisms, cells, etc. in the culture chamber B are not destroyed.

また、多孔板８は、培地は通過させるけれども、担体は
通過させないので、培養室Ｂ内の担体が培地調整室Ａ内
に入り込み、担体および微生物等が破壊されることはあ
り得ない。培養室Ｂの上部には、分離室Ｃがあり、ここ
では担体と培地とを分離する。培養槽１の頭頂部に設け
た円錐管４からは、この分離された培地のみを選択的に
抜出す。Furthermore, since the perforated plate 8 allows the medium to pass through but not the carriers, it is impossible for the carriers in the culture chamber B to enter the culture medium adjustment chamber A and destroy the carriers, microorganisms, etc. There is a separation chamber C above the culture chamber B, in which the carrier and the medium are separated. Only this separated culture medium is selectively extracted from the conical tube 4 provided at the top of the culture tank 1.

調整された新しい培地を培地調整室Ａから次々と培養室
に供給し、古くなった培地を培地抜出部りから抜出すの
で、長期間安定した培養が行なわれる。Since the new conditioned medium is successively supplied from the medium adjustment chamber A to the culture chamber and the old medium is extracted from the medium extraction section, stable culture is carried out for a long period of time.

〔発明の効果〕以上説明したように本発明によれば、担体法を利用した
培養槽での細胞または微生物の培養において、高効率培
養を実現することができる。[Effects of the Invention] As explained above, according to the present invention, highly efficient culture can be achieved in culturing cells or microorganisms in a culture tank using a carrier method.

【図面の簡単な説明】[Brief explanation of the drawing]

第１図は本発明の一実施例を示す図である。１・・・・・・培養槽、Ａ・・・・・・培地調整室、Ｂ
・・・・・・培養室、Ｃ・・・・・・分離室、Ｄ・・・
・・・培地調整室、２・・・・・・ジャケット、３・・
・・・・逆円離調、４・・・・・・円錐管、５・・・・
・・バッフル、６・・・・・・撹拌翼、７・・・・・・
撹拌翼駆動装置、８・・・・・・多孔板、９・・・・・
・センサ群、１０・・・・・・スパージャ、１１・・・
・・・培地供給口、１３・・・・・・培地抜牙１図FIG. 1 is a diagram showing an embodiment of the present invention. 1... Culture tank, A... Culture medium adjustment room, B
...Culture room, C...Separation room, D...
...Medium adjustment room, 2...Jacket, 3...
...Reverse circular detuning, 4... Conical tube, 5...
... Baffle, 6... Stirring blade, 7...
Stirring blade drive device, 8... Perforated plate, 9...
・Sensor group, 10... Sparger, 11...
...Medium supply port, 13...Medium extraction 1 diagram

Claims (1)

Translated fromJapanese

【特許請求の範囲】[Claims]１、担体に微生物あるいは細胞を付着させ培養槽内で浮
遊培養させる培養装置において、該培養槽下部に培地供
給口、通気ガス供給部、および撹拌部を有する培地調整
室を設け、該培養槽内の該培地調整室上部に担体に付着
された微生物あるいは細胞を培養する培養室を設け、該
培地調整室と該培養室との間は担体を通過させない程度
の孔寸法を有する多孔板で仕切り、該培養室の上部には
上部にいくほど径が大なる逆円錐状の分離室を備え、該
分離室内の培地を抜出す培地抜出部を該培養槽上部に設
けたことを特徴とする培養装置。1. In a culture device in which microorganisms or cells are attached to a carrier and cultured in suspension in a culture tank, a culture medium adjustment chamber having a culture medium supply port, an aeration gas supply part, and a stirring part is provided at the bottom of the culture tank, and the inside of the culture tank is A culture chamber for culturing microorganisms or cells attached to the carrier is provided above the medium adjustment chamber, and the medium adjustment chamber and the culture chamber are partitioned with a perforated plate having a hole size that does not allow the carrier to pass through, A culture characterized in that the upper part of the culture chamber is provided with an inverted conical separation chamber whose diameter increases toward the top, and a medium extraction part for extracting the medium in the separation chamber is provided at the upper part of the culture tank. Device.