【発明の詳細な説明】発明の名称ケゝル測光走査装置技術分野本発明は測光装置に係り、よシ詳しくは電気泳動分析の結果として得られたゲル（１月Ｅｉ／Ｙ）の測光走査装置に関る。[Detailed description of the invention]name of inventionKeel photometric scanning deviceTechnical fieldTECHNICAL FIELD The present invention relates to a photometric device, and more particularly to a photometric device for measuring gels obtained as a result of electrophoretic analysis.(January Ei/Y) related to the photometric scanning device.
技術水準ケ゛ル状生物学的製剤の電気泳動分析法は生物学、医学及び選別における主要な研究法の一つである。technical levelElectrophoretic analysis of shelled biological products is a major field in biology, medicine and screening.It is one of the research methods.
この方法の広汎な利用は高い分解能、単純さ及び必要な装置の低コストによるほか、多数（数十あるいは百数十）の生物学的製剤の並行的分析を行なう可能性にもよる。これはゲル測光走査装置に、特に、生産性、コスト、小型性、及び高い測定精度保証の可能性について、より厳格な要求を課す。The widespread use of this method is due in large part to its high resolution, simplicity and low cost of the required equipment.or the possibility of performing parallel analysis of large numbers (dozens or even hundreds) of biological products.It depends. This is especially important for gel photometric scanning devices due to their high productivity, cost, compactness, andimpose stricter requirements on the possibility of guaranteeing measurement accuracy;
加えて、グル長を伸ばす目的でケ゛ル状生物学的製剤の電気泳動分析法を改良する傾向が、それが分析能にするので、顕著になってきている。長さ４０〜５０Ｃｒｎまでのそうしたケ゛ルの測光走査装置の開発は根本的に新しい技術概念を必要とする。というのは、そうした目的のための慣用の装置は、とシわけ、走査中のケ゛ルの均一な移動動作を与えることがかなシ困難なので、かさばシ（長さ１００〜１２０ｃｍまで）、従ってよシ複雑な設計とよシ高い費用がかかるからである。In addition, improvements are being made to the electrophoretic analysis method for shell-shaped biological products in order to increase the length of the glue.The trend towards increasing the analytical capabilities is becoming more pronounced. Length 40~50CThe development of such photometric scanning devices up to RN required fundamentally new technological concepts.Essential. This is because the conventional equipment for such purposes isSince it is difficult to give a uniform movement to the scale of the scale, a bulky scale (length 100 to 120 cm), therefore requiring a more complicated design and higher cost.be.
光路に沿って前後に並べて配置された絞）とセル内のグルとを通して光線を送るモノクロメータを含み、セルがマイクロメータネノを有する手動式駆動手段で光線に関して移動せられ、そして光が測光器で受領されるグル測光走査装置が知られている（ Ｇ、 Ｍａｕｒｅｒ ％ ｒディスク−電気泳動」、１９７１年、Ｍ工Ｒ／＃ブリッジユバウス、モスクワ、１０３〜１０４頁、第２９図参照）。The light beam is sent through the aperture (apertures arranged one behind the other along the optical path) and the glue in the cell.Contains a monochromator, the cell has a micrometer scale and a manual drive meansA photometric scanning device is known in which the beam is moved relative to a line and the light is received by a photometer.(G. Maurer %r disk electrophoresis, 1971,M Eng R/# Bridge Yubaus, Moscow, pp. 103-104, see figure 29).
この装置ではセルは手動式駆動手段で往復運動せられる一定直径の石英管の形に作られている。In this device, the cell is in the form of a quartz tube of constant diameter that is reciprocated by a manual drive means.It is made.
この装置の石英管は長さ６ｃｒｎなので１個のグルだけを管内に置くことができる。これは頻繁なセルの再充填を必要とし、従って生産性が悪い。The length of the quartz tube in this device is 6 crn, so only one glue can be placed inside the tube.Ru. This requires frequent cell refilling and is therefore unproductive.
装置に高価な石英管を用いると装置をかなシコスト高にする。異なる直径のグルを゛測光走査するためには各々の直径の石英管の組を持つ必要があシ、それはそうした装置のコストをかなり増大させる。異なる直径のダルを調べるために単一の石英管を用いる場合には、石英管内に置かれた時のグルの変形又は石英管内のグルのゆるい一致が誤走査を起こしやすい。If an expensive quartz tube is used in the device, the cost of the device will be considerably high. Glue of different diametersIn order to perform photometric scanning, it is necessary to have a set of quartz tubes of each diameter;significantly increases the cost of such equipment. Single to examine dals of different diametersWhen using a quartz tube, deformation of the glue when placed in the quartz tube orLoose matching of the glue is likely to cause mis-scanning.
加えて、この装置でセルを動かす手動式駆動手段の使用は、結果として、ケ゛ル走査の低い生産性及び、セルをマクイロメータ式バーニアで読まれたある地点そして同じ距離を移動する複数の反復操作における誤差の高い可能性をもたらす。In addition, the use of manual drive means to move the cells in this device results in cellLow scanning productivity and certain points where the cells were read with a micrometer vernierMoving the same distance results in a high probability of error in multiple repeated operations.
光路に沿って前後に湛べて置かれた絞シとセル内のケ゛ルとを通して光線を送るモノクロメータを含み、セルが電気機械的駆動手段で光線に関して動かされ、そして光が測光器で受領されるケ゛ル測光走査装置が提案されている（ Ｂｏｏｋｌｅｔ ｌ５Ｃｏ ＩｎｓｔｒｕｍｅｎｔｓＳｐｅｃｉａｌｉｔｉｅｓ Ｃｏｍｐａｎｙ ）、米国、リンカーン、ネブラスカ １１特殊計器（Ｉｎｓｔｒｕｍｅｎｔｓ ｗｉｔｈ ａ ｄｉｆｆｅｒｅｎｃｅ）”。The light beam is sent through the diaphragm placed in front and back along the optical path and the cell inside the cell.a monochromator, in which the cell is moved relative to the light beam by electromechanical drive means;A cell photometric scanning device in which the light is received by a photometer has been proposed (Booklet l5Co InstrumentsSpecialities Company), Lincoln, Nebraska, USA 11 Special Instruments (Instrum)ents with a difference)”.
２０〜２１頁、１９７９年５月参照）。20-21, May 1979).
この装置では、セルは電気機械的駆動手段の作用で光線に関して交換（往復運動）されるように並べられた長さ２５ｃ１ｎの石英管を含む。In this device, the cells are exchanged (reciprocating) with respect to the light beam under the action of electromechanical drive means.) quartz tubes with a length of 25 c1n are arranged.
長さ２５αの石英管の使用は再充填なしで２個の長さ１２ｃｍ０ケ゛ルの走査を可能にし、電気機械的駆動手段の装備は上記の装置に較べてよシ高い生産性及びケ・ル走査におけるよシ小さい誤差を可能にする。The use of a 25α long quartz tube allows scanning two 12 cm long 0-kel scales without refilling.The provision of electromechanical drive means allows for higher productivity andAllows for much smaller errors in cell scanning.
しかし生産性の増加は大規模な分析操作には未だ不十分である。セル長の更々る増加は解決にならない。However, the increase in productivity is still insufficient for large-scale analytical operations. Increased cell lengthIncrease is not the solution.
というのは、それは全長にわたって均一な光学的特性を示すべきかなシ長い石英管を製造し操作するという問題がからんでいるからである。石英管の形に作られたセルと結びついたこの装置を改良するもう一つの難しさは、非常に長いセルを動かす往復駆動手段の設計の複雑さにある。さらに、高価な石英管のセルとしての使用はこの種の装置のコストを下げることを許容しない。This is because it should exhibit uniform optical properties over its entire length.This is because the problems involved in manufacturing and manipulating the tubes are involved. made in the shape of a quartz tubeAnother difficulty in improving this device in conjunction with long cells is that very long cellsThis lies in the complexity of the design of the reciprocating drive means used to move it. Furthermore, as an expensive quartz tube celldoes not allow to reduce the cost of this type of equipment.
この装置における石英管の大きな長さは往復運動駆動手段を非常に複雑にし、全体として装置の設計をかなシ複雑にする。The large length of the quartz tube in this device makes the reciprocating drive means very complex and theThis makes the design of the device extremely complex.
長さ２５ＣｒＩＬのセルの精確な運動のためには装置は長さ５０ｃＩｒＬの往復運動駆動手段を装備するので、やけシ装置の寸法は太きい。生化学及び医学の試験を遂行する場合、ただ一種の分析のためにさえ数多くの装置を使用するので、そうした装置が小型であることは非常に重要である。For precise movement of a cell with a length of 25 CrIL, the device must be moved back and forth with a length of 50 CrIL.Since it is equipped with a motion drive means, the size of the burner device is large. Biochemistry and Medical ExamWhen carrying out experiments, a large number of devices are used even for just one type of analysis, soIt is very important that such devices be small.
前述の装置とちがって、この装置は円柱状及び角柱状の両方のケ゛ルを走査することを可能にする。しかしながら、この装置のセルは所定の寸法なので、グルの横断面寸法の範囲は限定され、それがいろいろな種類の電気泳動分析で得られる電気泳動パターンを走査するためにこの装置を使用することを困難にする。その結果、装置の操作能力が限定され、それが今ではケ゛ルを用いる分析的研究における大きな不利になっている。Unlike the previously described devices, this device scans both cylindrical and prismatic cells.make it possible. However, since the cells of this device are of a predetermined size,There is a limited range of cross-sectional dimensions that can be obtained with different types of electrophoretic analysis.making it difficult to use this device to scan electrophoretic patterns. theAs a result, the ability to manipulate the equipment is limited, which is now limited to analytical studies using cables.This puts them at a huge disadvantage.
５この装置では、前述したものと同様に、ケ８ル及びセルの円筒表面での光の強い散乱を除去することが可能ではない。加えて、ケ゛ル表面の損傷は偽ピークとして記録される。この結果、この装置では、走査結果における誤差が光散乱及びケ゛ルの表面損傷の負の影響と結びついており、前述の装置と較べた測定精度の改良が存在しない。5This device, similar to the one described above, uses strong light on the cylindrical surface of the cell and the cell.It is not possible to eliminate scattering. In addition, damage to the cell surface can cause false peaks.recorded. As a result, with this device, errors in the scan results are caused by light scattering andThis is associated with the negative effects of surface damage and improved measurement accuracy compared to the previously described devices.There is no good.
上述の二つの装置ではケゝルの不均一な位置やセルとのゆるやかな一致に伴なう誤差を除去することが可能でない。In the two devices mentioned above, due to the uneven position of the cell and the loose alignment with the cell,It is not possible to eliminate errors.
発明の概要本発明は、グル走査の生産性及び測定精度を実質的に改良し、コストを低下し、装置の操作能力を改良するような仕方でケ゛ルを収容するセルを作った、簡単かつ小型のゲル測光走査装置を提供することを課題とする。Summary of the inventionThe present invention substantially improves guru scanning productivity and measurement accuracy, lowers costs, andA simple method of constructing a cell to house the cable in such a way as to improve the operational capabilities of the device.An object of the present invention is to provide a compact gel photometric scanning device.
これは、光路に沿って前後に並べて置かれた絞りとセル内のグルを通して光線を送るモノクロメータを含み、セルが電気機械的駆動手段の作用で動かされ、そして光が測光器で受領されるケ゛ル測光走査装置であって、本発明に依シ、セルが二枚の重ね合わされた円板を含み、各円板がその少なくとも一側面に相互に一致する溝を有し、円板を互いに重ね合わせた時にそれらの溝がその中にケ゛ルを入れる少なくとも１個の区画室を構成する装置によって達成される。This directs the light rays through apertures placed one behind the other along the optical path and through a lens inside the cell.the cell is moved under the action of electromechanical drive means;A cell photometric scanning device in which light is received by a photometer, in which the cell isincludes two superimposed disks, each disk coinciding with the other on at least one side thereofWhen the discs are stacked on top of each other, these grooves allow the cable to be inserted into them.This is achieved by means of a device constituting at least one compartment.
光線の通過用にセルの両方の円板の溝の底の側方に開口を設けることが好ましい。Preferably, openings are provided at the sides of the bottom of the grooves in both discs of the cell for the passage of the light rays..
本発明に依るこの構成の装置はゲルをセルに充填し、セルを装置に装着するのに伴なう補助操作の合計数の減少に依って走査の生産性向上を可能にする。A device of this configuration according to the invention is used to fill a cell with gel and to attach the cell to the device.This allows for increased scanning productivity due to the reduction in the total number of auxiliary operations involved.
セルに数個のグルを入れるので、よシ長時間の走査を行ない、その間に操作員が他の操作に注意を向けることを可能にする。本発明に依る装置に数個（例えば４個）のグルを入れるだめの時間は前述した従来技術の装置に１個のグルを入れるのに必要な時間と実質的に等しい。Since several glues are placed in the cell, a very long scanning time is required, during which the operatorAllows attention to be directed to other operations. In the device according to the invention several (e.g. 4)The time required to insert 1 glue into the prior art device described above is as follows:substantially equal to the time required to
加えて、相互に一致する溝を有する２枚の円板の形のセルの装備は、セルの設計によってグルをセルに均一に合致させる可能性を与え、ケ゛ルの軸変形を防止して、測定精度の改良を可能にし、そして走査中における正確に再現可能なダルの位置が保証される。との理由から、本発明に依る装置におけるグル走査は、結果の高い再現性及びすべての測定において一定でちる正確さを可能にする。これは、ｒル分析にとって定量的比較の可能性を有することは決定的なことなので、重要である。In addition, the equipping of the cell in the form of two discs with mutually coincident grooves makes the design of the cellgives the possibility of matching the glue uniformly to the cell and prevents axial deformation of the cell.This allows for improved measurement accuracy and accurately reproducible dullness during scanning.Location is guaranteed. For this reason, the guru scanning in the device according to the invention results inenables high reproducibility and consistent accuracy in all measurements. this is, since it is crucial to have the possibility of quantitative comparisons for ruru analysis, it is importantIt is essential.
加えて、この構成のセルは石英管を使用する必要を省き、従って装置のコストを下げることを可能にする。In addition, this configuration of cells eliminates the need to use quartz tubes, thus reducing equipment costs.allow it to be lowered.
セル円板の符号溝で構成されたセル区画室の作業長における実質的な増大は、大きな長さのケ゛ルを走査し従って装置の操作能力をかなシ改良することを可能にする。さらに、装置の能力はセルが符号溝を有する２枚の円板の形に作られていることによっても改良される。この便宜さは異なる直径のケ゛ルを走査することを可能にするので、装置はケ゛ルの諸種の電気泳動分析の結果として得られる電気泳動・ぐターンを走査するために使用できる。The substantial increase in the working length of the cell compartment made up of the code grooves of the cell disc isIt is possible to scan cables of large lengths, thus greatly improving the operating ability of the device.do. Furthermore, the capability of the device is that the cells are made in the form of two discs with code grooves.It can also be improved by This convenience allows scanning cells of different diameters.Because the device enables electrophoretic analysis of various types of cells, the deviceCan be used to scan aerophores.
本発明に依るセルの構造的態様はケ゛ル走査装置の寸法の実質的な減少を可能にし、それによって全体としての装置の寸法を決めるセル寸法はセル区画室の作業長の３倍小さい。The structural aspect of the cell according to the invention allows a substantial reduction in the dimensions of the cell scanning device.The cell dimensions, which determine the dimensions of the device as a whole, are determined by the operation of the cell compartment.3 times smaller than the length.
本発明に依る装置におけるセルの各円板の両側に溝が作られる場合、円板のもう一方の側に作られた溝は光の通過用の開口として働くことができ、そして円板をこれらの側がお互いに向い合うように重ね合わせたとき、それらもその中にケ゛ルを入れる少なくとも１個の区画室を構成することができる。If grooves are made on both sides of each disc of the cell in the device according to the invention, the discs can also beThe groove made on one side can act as an aperture for the passage of light, and the discWhen stacked so that these sides face each other, they also fit inside.At least one compartment can be configured to contain the module.
この態様は装置の操作能力を改良すること、即ち、異なる直角断面形状、例えば矩形を有するケ゛ルをも操作することを可能比する。This aspect improves the operational capabilities of the device, i.e. different orthogonal cross-sectional geometries, e.g.It is also possible to manipulate cells with rectangular shapes.
板状のケ゛ルにおける電気泳動分析が最近広汎に使用されるようになってきたので、本発明に依る装置におけるこの能力の付与は非常に重要である。そうした場合、走査は板状物から個別電気泳動試料を切断後に行なわれる。Electrophoretic analysis using plate-shaped cells has recently become widely used.The provision of this capability in the device according to the invention is therefore of great importance. such a placeIn this case, scanning is performed after cutting the individual electrophoresis samples from the plate.
セル円板の溝は各円板の周辺に沿って作られることが好ましい。Preferably, the grooves in the cell discs are made along the periphery of each disc.
この態様のセルは、セルをモノクロメータからの光線に関して動かすために単純で安価な電気機械的駆動手段を使用することができるので、装置を実質的に単純化することを可能にする。This embodiment of the cell is simple in order to move the cell with respect to the light beam from the monochromator.can use inexpensive electromechanical drive means, making the device substantially simpler.make it possible to
ケ゛ル物質と類似の屈折率を有する液体で満たされ、光学的に透明な光線の入口及び出口の窓を有し、そして、ケ゛ルを中に入れる区画室を有するセルの円板の一部が浴の液体に沈められるような仕方で絞シと測光器の間に並べて置かれた、浴を、装置に装備することが好ましい。Optically transparent light beam entrance filled with a liquid with a refractive index similar to that of the bulk materialand an exit window, and a cell disc having a compartment into which the cage is placed.placed side by side between the diaphragm and the photometer in such a way that it was partially submerged in the bath liquid.Preferably, the apparatus is equipped with a bath.
この装備は走査結果における冴゛ルの表面損傷の影響及び光散乱を実質的に減少することを可能にする。This equipment substantially reduces the effects of surface damage and light scattering on the scanning results.make it possible to
この結果として、本発明に依る装置は、よシ低い光散乱バックグラウンドの結果として改良された測定感度を有し、グルの表面損傷によって起きる走査記録における偽ピークの増幅が実質的に低下する。As a result of this, the device according to the invention results in a lower light scattering background.It has improved measurement sensitivity and eliminates scanning records caused by surface damage of the glue.The amplification of spurious peaks caused by
図面の簡単な説明９以下本発明を添付図面に描かれた特別の態様を参照して詳細に説明するが、第１図は本発明に依るケ゛ル測光走査装置の一部縦断面図の全体図、第２図は第１図に示した装置のセルの円板のうち１個の内側表面の平面図、第３図は第１図の装置のセル円板の一部を沈めた液体の浴を示す第１図の矢印Ａに沿って見た図、第４図は本発明に依る装置のもう一つの態様のセル円板の一部の縦断面図、第５図は円板の反対側をお互いに重ね合わせた点を除き第４図と同じ図である。Brief description of the drawing9The present invention will now be described in detail with reference to specific embodiments illustrated in the accompanying drawings.FIG. 1 is an overall view of a partial longitudinal cross-sectional view of a cell photometric scanning device according to the present invention;FIG. 2 is a plan view of the inner surface of one of the cell disks of the device shown in FIG. 1;Figure 3 shows the liquid bath in which a portion of the cell disk of the apparatus of Figure 1 is submerged, indicated by the arrow A in Figure 1.FIG. 4 shows a portion of the cell disk of another embodiment of the device according to the invention.Longitudinal cross-sectional view ofFIG. 5 is the same view as FIG. 4, except that the opposite sides of the disks are superimposed on each other.
好ましい態様本発明に依るケ゛ル測光走査装置はモノクロメータ−（第１図）を含み、そこから光線が、光路に沿って前後に並べて置かれた絞シ２、液体５で満たされた浴４の光学的に透明な入口窓３、セルフに入れられたケ・ル６、及び浴４の光学的に透明な出口窓８を通過し、光は測光器９で受領される。Preferred embodimentThe cell photometric scanning device according to the invention includes a monochromator (FIG. 1), from whichThe light beam passes through an aperture 2 placed one behind the other along the optical path, and a bath 4 filled with a liquid 5.optically transparent entrance window 3, a self-contained cell 6, and an optically transparent entrance window 3 of the bath 4.Passing through a transparent exit window 8, the light is received by a photometer 9.
セルフは金属例えばアルミニウム合金製の２枚の重ね合わされた円板１０．１１の形に作られ、円板は内側表面１２（第２図）に符号し合った溝１３を有する。Self consists of two overlapping disks made of metal, e.g. aluminum alloy10.11The disk is made in the shape of , and has congruent grooves 13 on its inner surface 12 (FIG. 2).
円板１０．１１の溝１３は円板の周辺に沿って作られている。The grooves 13 in the disc 10.11 are made along the periphery of the disc.
円板の中心から種々に隔置して設けられた数個の溝あるいは単一の螺旋状溝さえを有するいろいろな態様の装置を使用することができる。several grooves or even a single helical groove at various distances from the center of the discVarious embodiments of the device can be used.
円板１０．１１（第１図）をお互いに重ね合わせると溝１３（第２図）はその中にグル６を入れる区画室１４（第１図）を構成する。この態様の装置では４個のグル６が入れられる雫−の区画室１４が構成される。When the discs 10.11 (Fig. 1) are superimposed on each other, the groove 13 (Fig. 2) is located inside them.A compartment 14 (FIG. 1) in which the glue 6 is placed is constructed. In this embodiment of the device, there are fourA droplet compartment 14 into which the glue 6 is placed is constructed.
数個の区画室を有する円板を持つ装置を作ることもできる。It is also possible to create a device with a disk having several compartments.
両方の円板１０．１１は溝１３（第２図）の底の側にそれぞれ開口１５．１６を有する。一体性を保つだめに円板１０．１１に橋部１７を設ける。Both discs 10.11 each have an opening 15.16 on the bottom side of the groove 13 (FIG. 2).have To maintain integrity, the disk 10.11 is provided with a bridge 17.
セルフの円板１０．１１（第１図）は光線に関して回転（運動）させる駆動手段１９の出力軸１８に取シ付けられる。Self's disk 10.11 (Fig. 1) is a driving means for rotating (moving) with respect to the light beam.It is attached to the output shaft 18 of No. 19.
セルフの２枚の円板１０．１１間にいろいろな厚さのワッシャ（図示せず）を挿入することによって円板１０．１１の間の間隔を変えて区画室１４の横断面寸法を変化させ、そうして広汎な横断面寸法にわたるグル６を走査することを可能にすることができる。Insert washers of various thicknesses (not shown) between the two discs 10 and 11 of the selfie.The cross-sectional dimensions of the compartment 14 can be changed by changing the spacing between the discs 10.11 by, thus making it possible to scan the guru 6 over a wide range of cross-sectional dimensions.can do.
上記のように、浴４は液体５で満たされる。この”液体はその屈折率がグル６の物質の屈折率と類似であるように選択される。こうして、この態様、においてポリアクリルアミドや寒天のグル６を調べるべき場合には液体５は水である。As mentioned above, bath 4 is filled with liquid 5. This liquid has a refractive index of 6It is chosen to be similar to the refractive index of the material. Thus, in this aspect, theWhen the glue 6 of lyacrylamide or agar is to be investigated, the liquid 5 is water.
浴４は、絞シ２と測光器９の間に、ケ゛ル６を入れる区画室１４（第１図）を有するセルフ（第３図）の円板１０．１１の一部が液体５中にあるような仕方で、設置する。The bath 4 has a compartment 14 (FIG. 1) in which the scale 6 is placed between the diaphragm 2 and the photometer 9.in such a way that part of the disc 10.11 of the self (Fig. 3) is in the liquid 5,Install.
第３図に見られるように浴４は弓形（セグメントの形）である。これはセルフの形状で決められ、かつ液体が高価な添加剤を含むかもしれない場合の適用において液体を節約することを可能にする。As can be seen in FIG. 3, the bath 4 is arcuate (segment-shaped). This is selfIn applications where the shape is determined and the liquid may contain expensive additives.allows you to conserve liquid.
絞り２（第１図）、浴４、及びセルフは取シ外し可能な遮光フード２１を有する・・ウノング２０に収容されている。フード２１は、セルフの円板１０゜１１を取υ外し可能に取シ付けた駆動手段１９の出力軸１８用の開口を有してい・る。The aperture 2 (Fig. 1), the bath 4, and the self-container have a removable light-shielding hood 21....It is housed in Unong 20. The hood 21 has a self-disc plate 10°11.It has an opening for an output shaft 18 of a removably mounted drive means 19.
測光器９の出力はチャート紙を有する記録計２２に電気的に接続されており、走査記録２３がチャート紙に記録され、そのピークが電気泳動試料の調査物質の位置を評価するために使用される。The output of the photometer 9 is electrically connected to a recorder 22 having a chart paper.The inspection record 23 is recorded on chart paper, and the peak indicates the position of the investigated substance in the electrophoresis sample.used to evaluate the position.
前述された本発明に依る装置の態様ではセルフの円板１０．１１をお互いに重ねたとき溝１３で形成される区画室１４は円筒状のケ゛ル６を走査するように設計されている。In the embodiment of the device according to the invention described above, the self-discs 10, 11 are stacked on top of each other.The compartment 14 formed by the groove 13 is designed to scan the cylindrical cage 6.has been done.
しかしながら、溝の形状を変えることによって異なる輪郭のグルを走査することができる。However, it is possible to scan grooves with different contours by changing the shape of the grooves.Can be done.
第４図及び第５図は一方の側２８及び反対側２９にそれぞれ符号溝２６．２７を有する円板２４゜２５を重ねることによって形成された本発明に依る装置のセルフを示す。この構成のセルフは異なる形状のケ゛ルー、のその適用を可能にする。Figures 4 and 5 show sign grooves 26 and 27 on one side 28 and the opposite side 29, respectively.Cell of the device according to the invention formed by superimposing disks 24° 25 withIndicates the This configuration self allows its application of keyholes of different shapes,.
こうして、セルフの円板２４．２５の而２８をお互いに向かい台わ亡て重ねた場合、溝２Ｇはその中に円筒状ケ゛ル６を入れる区画室３０を形成し、円板２４．２５の他方の外側の面２９に作られた溝２７は光の通路として働く。In this way, if the self disks 24, 25 and 28 are stacked upside down facing each other,In this case, the groove 2G forms a compartment 30 in which the cylindrical shell 6 is placed, and the disc 24.A groove 27 made in the other outer surface 29 of 25 serves as a light passage.
セルフの円板２４．２５（第５図）の面２９をお互いに向かい合わせて重ねた場合、溝２７はその中に矩形の直角断面形状のケ゛ル３２を入れる区画室３１を形成し、円板２４．２５の反対側の外側の面２８は光の通路として働く。When the self disks 24 and 25 (Fig. 5) are stacked with their surfaces 29 facing each other,In this case, the groove 27 forms a compartment 31 into which a cage 32 of rectangular cross-section is placed.The opposite outer surface 28 of the discs 24, 25 serves as a light passage.
数個の区画室３０．３１があってよい（第４図）。There may be several compartments 30,31 (FIG. 4).
本発明に依るケ８ル測光走査装置は以下のように操作する。The cell photometric scanning device according to the invention operates as follows.
遮光フード２１（第１図）を取シ外した後、セルフ（第１図）の両方の円板１０．１１又は両方の円板２４ 、２５ （第４図）を駆動手段１９の軸１８から取り外す。ケ゛ル６又は３２を一方の円板１０又は２４の溝１３即ち２６（第４図）又は２７（第５図）に入れて、少なくとも２〜３ミリメートルの間隔をケ゛ル６及び３２と橋部１７の間に残すようにする。After removing the light-shielding hood 21 (Fig. 1), remove both discs 10 of the self-container (Fig. 1).．． 11 or both discs 24, 25 (Fig. 4) are removed from the shaft 18 of the drive means 19.Remove. The cable 6 or 32 is inserted into the groove 13 or 26 of one of the discs 10 or 24 (Fig. 4).) or 27 (Fig. 5), with a distance of at least 2 to 3 mm.6 and 32 and the bridge portion 17.
次いで円板１１又は２５を重ね合わせて、両方の溝１３ 、２６ 、２７が円筒状グル６用の区画室１４゜３０及び矩形横断面形状のグル３２用の区画室３１を形成し、かつ両方の円板１０．１１又は２４゜２５の橋部１７が符合（一致）するようにする。Next, the disks 11 or 25 are stacked so that both grooves 13, 26, and 27 are cylindrical.A compartment 14° 30 for the shaped glue 6 and a compartment 31 for the glue 32 with a rectangular cross section are provided.and the bridge portions 17 of both discs 10, 11 or 24°25 match (match).so that
水を浴４（第１図）に注いで水位が窓３，８の僅かに上になるようにする。ケ゛ル６又は３２を中に入れた組み付はセルフを駆動手段１９の軸１８に取シ付けて、モノクロメータ１からの光線が開口１５に来るようにする。それから遮光フード２１を取シ付け、測光器９の基準線をセットする。駆動手段１９並びに記録計２２のチャート紙移送用駆動手段を起動し、ケ゛ル６又は３２の測光走査を行なう。モノクロメータ１（第１図）からの光線は０．２ ｍｍ幅の狭い光線を切シ抜く絞シ２を通過し、読いて順に、浴４の入口窓３、水、開口１５、グル６、開口１６、水、浴４の出口窓８を通過し、そして測光器９に入射する。グル６を通過する光線の吸収は記録計２２に記録される。ケ゛ルで分析されている生物学的対象の帯域の存在位置は記録計で走査記録におけるピーク２３として記録される。セルフの円板が完全な回転を行なった後、走査工程を終える。矩形直角断面形状のケ゛ル３２（第５図）の走査も同様な仕方で遂行される。Pour water into bath 4 (FIG. 1) so that the water level is slightly above windows 3,8. KeiWhen assembling with the lever 6 or 32 inside, attach the self to the shaft 18 of the drive means 19., so that the light beam from the monochromator 1 comes to the aperture 15. Then the blackout fu21 and set the reference line of the photometer 9. Drive means 19 and recorderStart the chart paper transport drive means 22 and perform photometric scanning of scale 6 or 32.cormorant. The light beam from monochromator 1 (Figure 1) is cut into a narrow beam with a width of 0.2 mm.It passes through the drawing diaphragm 2, and in order, the entrance window 3 of the bath 4, the water, the opening 15, the glue 6, and the opening are opened.At the mouth 16, the water passes through the exit window 8 of the bath 4 and enters the photometer 9. Through Guru 6The absorption of the passing light beam is recorded on a recorder 22. Biological substances being analyzed in the cellThe location of the band of interest is recorded by the recorder as peak 23 in the scan record.. After the self-disc completes its rotation, the scanning process is completed. Rectangular right angle cross sectionScanning of the shaped cell 32 (FIG. 5) is accomplished in a similar manner.
区画室の直角断面寸法よシ大きい寸法のグルをセルに入れるために、グルを固定するのに十分な厚さのワッシャを円板の間に挾む。Fix the glue in order to insert the glue into the cell with dimensions larger than the perpendicular cross-sectional dimensions of the compartment.Place a washer between the discs that is thick enough to
本発明に依る装置の特別の適用では、各々長さ４αの４個の例えば円筒状のケ゛ル又は長さ５０ｃｌｒＬの１個のグルの連続走査がセルの再充填なしで可能である。セルの円板における溝は直径１７ｃｍに作られる。In a particular application of the device according to the invention, four, for example cylindrical, cases each of length 4α are used.Continuous scanning of one glue of length 50 clrL is possible without cell refilling.Ru. The groove in the disk of the cell is made with a diameter of 17 cm.
走査中のグルを通過する光線の幅は通常０１〜０．５ｍｍであるので、直径１７ｃ７ｒＬの円弧に沿うグルの曲率は測定精度に影響しない。The width of the light beam passing through the guru during scanning is usually 01-0.5 mm, so the diameter 17The curvature of the guru along the arc of c7rL does not affect measurement accuracy.
本発明に依る装置の適用は、他の条件が同じとして、ケ゛ル走査の正確さを１．５〜２倍に改良し、補助的操作の数の３〜４倍の減少を達成し、そして装置のコストを４分の１に下げることを可能にする。The application of the device according to the invention, other things being equal, increases the accuracy of the cell scan by 1.Achieved a 5-2x improvement, a 3-4x reduction in the number of auxiliary operations, and a 3-4x reduction in equipment cost.This makes it possible to reduce the cost by a quarter.
産業上の利用可能性本発明に依るグル測光走査装置は、グル例えばポリアクリルアミドや寒天のグルの電気泳動ノｅターンを定量的に評価する大規模な試験を行なう微生物学的工場やクリニックで、並びに、農業における選別操作の遂行に、利用することができる。Industrial applicabilityThe glue photometric scanning device according to the invention is suitable for glues such as polyacrylamide and agar.A microbiological factory that conducts large-scale tests to quantitatively evaluate the electrophoretic e-turn ofIt can be used in agriculture and clinics as well as in carrying out sorting operations in agriculture.Ru.
手続補正書（方式）昭和５７年１１月Ｃ日特許庁長官 若 杉 和 夫 殿１ 事件の表示ＰＯＴ／５Ｕ８１１０００１５２ 発明の名称ゲル測光走査装置３ 補正をする者事件との関係 特許出願人名称 インスティチュト ボタニキ エーエヌカザクスコイ ニスニスアール４代理人住所 〒１０５東京都港区虎ノ門−丁目８番１ｏ号５ 補正命令の日付自発補正６ 補正の対象（１） 明細書の翻訳文（２）請求の範囲の翻訳文７ 補正の内容（ＩＯ２）別紙の通り（内容に変更なし）（２）国 際 聰 杏 却 牛１１１１ｅｌｌｌａ１４１１１１１ Ａ１１１１１１．Ｉｌ＠。、。ＰＣｒ／５Ｕ８１１０００１５ジロー１０５１２％； ２０←１８０ ！？（１，）Ｂ、 Ｐ； ３７Ａ； ４１Ａ、 Ｂ； ＧｌＡ、 ＧＩＰ、 Ｇ２．７． Ｂ２Ｊｌ？Ｘ竹・。容、１３Ｂ）ｉ３６ｈ ｌ□１ａｌｓｅａｒｃｈＩｕＭｌｆ１Ｍ書１１ｌ１０１ｂ＊１ｔｌｂｌｌ會ｈｅｄｍｒｅ＊０会（ｉｏｌｅｅ＋’ｌａｌｎｃｌａｉｍｓｕｎｄｗ＾−ｃＭ＋）Ｃりｆｉ１１６１＋１ｗｌａｌｌｏｗｍ＠ｎａａａ＋＋ａｑｂ６Ｈ＠、−、、、、、，１１・（−口１１１１１１ｍｙｒａｇ―電＠１６１ｕｂｊａｄａ’＋ａ６１１ＩｎｏｔＩｎｕｒｅｄＩｏ−Ｍａｒｅｈｅ４ｂｙｔｈｎ＾−Ｍｅｒｆｆｙ、ｒｍｍ＄＋ｎｍ＋°ｍ−−、ｂＫａｕｍ＊ｍｅｙｎ−レｍｔｏｐａｎｔｅｌｔｈｅｍｔ＊ｒｎａｔ１６ａｍｌｓｐｍａｒｍａｎｍｄｏ＋ｍａｗｅｗれｈｔりｖＨ−−ｃ＋１ｂｍｒ嚇鴫＋ｕｌｎｗ＋ｕｃｈ ａｎ ｓ＋ｍ＋＋１１１７ ｎｏ ｍｅａｎｌｎｇ％１石ｂｅｎｄ＠ｎｍｌ 謬＠ｙｃｈ ｃａｎ Ｉｎ ｃＬｆｆｌｅｄ ＠一本草１．中−−ｍ１号＋ＶＡＴＩＯＮ！Ｗ１４！Ｍ Ｌｌｌｌ１丁Ｙ （ＩＰ ＩＩＩＶ！ＮＴｌ０Ｎ ｌ襲し＾ＣＫＩＮ（ｌ。Procedural amendment (formality)November C, 1981Mr. Kazuo Wakasugi, Commissioner of the Patent Office1 Display of incidentPOT/5U811000152 Name of the inventionGel photometric scanning device3 Person making the amendmentRelationship to the incident: Patent applicantName Institute Botaniki ANE Kazakuskoy Nisnisar4 agentsAddress: 5-8-1o Toranomon-chome, Minato-ku, Tokyo 105 Date of amendment orderspontaneous correction6 Target of correction(1) Translation of the specification(2) Translation of the scope of claims7 Contents of amendment(IO2) As attached (no change in content) (2)International Satoshi Anzu Ushi1111ella1411111 A111111. Il@. ,. PCr/5U81100015 Jiro 10512%; 20←180! ? (1,)B,P; 37A; 41A, B; GlA, GIP, G2.7. B2Jl? X Bamboo. Yong, 13B) i36h l□1alsearchIuMlf1Mbook11l101b*1tlbll meetinghedmre＊0 meeting (iolee+'lalncclaimsundw^-cM+) Crifi1161+1wllallowm@naaa++aqb6H@,-,,,,,,11・(-口111111myrag-electronic@161ubjada'+a611InotInuredIo-Marehe4bythn^-Merffy, rmm$+nm+°m--, bKaum*meyn-remtopantelthemt*rnat16amlspmarmanmdo+mawewrehtrivH--c+1 bmr menace +ulnw+uch an s+m++1117 no meanlng%1 stone bend @nml falsification @ych can In cLffled@Ipponso 1. Naka--m1 No.+VATION! W14! M Lllll 1-cho Y (IPIIIIV! NTl0N l attack ^CKIN (l.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/SE1981/000015WO1981002185A1 (en) | 1980-01-24 | 1981-01-23 | Electrohydraulic set device |
| Publication Number | Publication Date |
|---|---|
| JPS58500083Atrue JPS58500083A (en) | 1983-01-13 |
| JPS6245493B2 JPS6245493B2 (en) | 1987-09-28 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP50123781AGrantedJPS58500083A (en) | 1981-01-23 | 1981-02-23 | Gel photometric scanning device |
| Country | Link |
|---|---|
| JP (1) | JPS58500083A (en) |
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|---|---|---|---|---|
| US9295464B2 (en) | 2010-09-30 | 2016-03-29 | Ethicon Endo-Surgery, Inc. | Surgical stapler anvil comprising a plurality of forming pockets |
| WO2016132625A1 (en)* | 2015-02-18 | 2016-08-25 | シャープ株式会社 | Sample container, measurement method, and method for manufacturing sample container |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49127689A (en)* | 1973-04-07 | 1974-12-06 |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49127689A (en)* | 1973-04-07 | 1974-12-06 |
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|---|---|---|---|---|
| US9295464B2 (en) | 2010-09-30 | 2016-03-29 | Ethicon Endo-Surgery, Inc. | Surgical stapler anvil comprising a plurality of forming pockets |
| WO2016132625A1 (en)* | 2015-02-18 | 2016-08-25 | シャープ株式会社 | Sample container, measurement method, and method for manufacturing sample container |
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| JPS6245493B2 (en) | 1987-09-28 |
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