JPS5841352A - Automatic fraction collector - Google Patents

Abstract

PURPOSE:To achieve a fractional collection of intended components regardless of changes in the base line by fractional collection thereof as set peak fraction with a decision on the peak thereof detecting differential variations in the rise at the set fraction peak. CONSTITUTION:When receiving an injection marker signal indicating the start, a peak detector 3 detects the peak by setting a peak sensitivity. An LPE1 prevents spike noises or the like. The marker signal is amplified with an amplifier 5 to serve as input signal for a time setting device 2 and triggers the setting device 2. The setting device 2 is latched after the elapsing of a set time and then, when a peak set for fraction in a liquid chromatograph is detected, it is ANDed with a set time signal latched to output pulses for starting fractional collection. Therefore, no detection is performed how much the base line varies within the set time and if the time is set with a enough room initially until the base is stabilized, the detection of the fraction peak can be done accurately.

Description

Translated fromJapanese

【発明の詳細な説明】本発明框、液体タロマドグラフ【使用した自動分取装置
に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a frame and a liquid talomagraph [an automatic fractionating device used].

近年、液体クロマトグラフｋｅ用して微量な生理活性物
質等に＠１０回に渡って繰り返し分取し、単離する装置
が求められてｈるが、現在の装置で目的成分Ｏ分ｓｒ行
うに框、スタート信号からの時間経過により自−分取す
る方法、或ｖｓは一定レベルのスレッシュホールドレベ
ル！設定し、ソのレベル（［遣したらビータ判定１行い
自動分取する方法がある。In recent years, there has been a demand for equipment that uses liquid chromatographs to repeatedly fractionate and isolate trace amounts of physiologically active substances over 10 times, but it is difficult to carry out the separation of target components with the current equipment. A method of self-separation based on the passage of time from the start signal, or vs is a certain threshold level! There is a way to set the level ([1 beater judgment when used) and automatically separate the sample.

然し乍ら、前者に於てはクロマトグラフの各分取ビータ
発生時間に常に一定にする必畳があり。However, in the former case, it is necessary to always keep the generation time of each preparative beater of the chromatograph constant.

リテンションタイムがずれると目的成分ｉ分取できない
という欠点が６１Ｆ、また後者に於ては、タロマドグツ
７０ベースツインが変化した場合スレツシエ本−ルドレ
ベルＯ股定に対し目的成分がレベルに這しない場會又に
遺し過ぎる場合も生じ。If the retention time deviates, the target component cannot be fractionated. There are cases where too much is left behind.

仲々設定通９ｍ実九分取することができないという欠点
があった。There was a drawback that it was not possible to take a 9m 9m distance.

そこで、本発＠は叙上Ｏ欠点＃Ｃ艦本てなされた４ｏで
あり１分取装置に於て、スタートｙ表わすインジエクン
コンマーカー伽号を平滑化させるフイ火メーと、分取設
定ピークの立上り信号に至る間ｔｅｌｌ設定によシネ本
帯としてマスタする時間設定器と、設定した分取ビーク
Ｏ立上９黴分変化の増加分を検出するピーク検出器とか
らなり、ピークの立上りｔ検出してリテンションタイム
のずれ、ベースライン変化に関係なく目的睨分ｉ分取す
る装置ｔＩＩ供せんとするものである。Therefore, the present @ is the 4o made by O fault #C Kanmoto, and 1. In the preparative device, it is necessary to smooth the initial y representing the start y, and the preparative settings. It consists of a time setting device that masters as the cine main band by the tell setting until the peak rising signal, and a peak detector that detects the increase in the preparative beak O rising 9 minute change. It is intended to provide an apparatus tII that detects t and performs aliquoting for the purpose regardless of retention time deviations or baseline changes.

以下本発明の一実施＾ｔＳ＊−漏に従って詳述する。纂
１１１１框ビータの検出構成を示すブロック−で島９．
スタート！表わすインジエクシツンマーカー信号１１１
００〜５００１１１Ｂ　、　１１１７レヘルが一般的で
、該信号倉受けるとピーク検出器３ｒｉピーク感度の設
定によってビータ検出を行なうため２〜ｚｏｎｚｏロー
パスフィル！−１ｒａｍしてスパイタノイズ等の防止！
含めた保護対策を購じて憤る。また、前記ＨａＶのマー
カー信和時間設定器２０入力信号として兼用させるため
増巾６５にで増巾し時間設定器２に？リガーさせる。Hereinafter, one implementation of the present invention will be described in detail. Block 1111 showing the detection configuration of the frame beater - Island 9.
start! Indicator marker signal 111
00~500111B, 1117 level is common, and when the signal is received, the peak detector 3RI performs beater detection by setting the peak sensitivity, so 2~zonzo low pass filter! -1 ram to prevent spyter noise, etc.!
I'm angry because I bought the protective measures that were included. Also, in order to make it also serve as the input signal for the HaV marker signal time setter 20, it is amplified by the amplification 65 and sent to the time setter 2? Let it be rigged.

曽紀ト啼ガーさ九た時間設定器２ｒｉ、設定時間が経過
した後ラッチさ九、その後液体クロマトグラフの分権設
定ビータを検出するとラッチされた設定時間信号と共に
論場積ざｆＬ、分取開始パルスに出力させる。従って、
時間設定された時間内にベースラインが如何に変動して
も検出動作１行なわないため口めのベースラインが安定
するまでの時間を余裕ｔもってセットすれば分取ピーク
Ｏ検出は確実に行なうことが可能である。When the time setter 2ri is triggered, the time setter 2ri is latched after the set time has elapsed, and then when the decentralization setting beater of the liquid chromatograph is detected, the latched set time signal is set, and the separation is started. Output to pulse. Therefore,
No matter how much the baseline fluctuates within the set time, one detection operation will not be performed, so preparative peak O detection can be performed reliably by setting a margin of time for the first baseline to stabilize. is possible.

纂ＺＳａ分取処虐のメイムチャートｌ示し、纂３−に設
定されたビータの検出方法！示した説明−である、纂３
ｍＫ’ｌｋて、初めに立上りピークの検出感度設定を行
っておき、タロマトダラフｒ常時ス命ヤンニングしな、
がら微分してｖｈ虐、微分値Ｐと前の微分値Ｐ−１と比
験し、比鞍した微分値Ｐが微分値Ｐ−１より太き一場会
ピークの上昇遥！ｊＡ＃Ｃ入るが、最初に設定した検出
感度の大きさよりも大きいと判断した時、即ちＰ−ＣＰ
−１）＞検出感度設定値に違した時にピーク検出ｆ＃ｉ
号が出力され電磁弁８（纂ｌ−参照）が励磁されて設定
ピークの成分が分取される。Showing the Mayme chart of ZSa fractional treatment, how to detect the beater set in 纂3-! This is the explanation shown, Part 3
mK'lk, first set the detection sensitivity of the rising peak, and then set the detection sensitivity of Taromato Darafu r constantly.
The differential value P is compared with the previous differential value P-1, and the compared differential value P is thicker than the differential value P-1, and the peak rises far! jA#C enters, but when it is determined that the detection sensitivity is larger than the initially set detection sensitivity, that is, P-CP
-1) > Peak detection f#i when the detection sensitivity setting value is different
The signal is output, the solenoid valve 8 (see collection 1-) is excited, and the component of the set peak is fractionated.

以上詳１＠Ｃ説明したように本発明分取Ｍ置が、スター
トを表わすインジエクシ■ンマーカー信号を受けてから
設定した分取ビータが到達する間。As explained above in detail 1@C, the time period from when the preparative separation M device of the present invention receives the injector marker signal indicating the start until the preparative beater that has been set arrives.

取ピークの検出が先行の変動ピークの影響ｒ受けず正Ｗ
ＣでＩ！、設定ピークの分取やカラム切換え等を正１１
１に自動化でき、高Ｈｆ且つ低コストの分取装置！提供
できる。The detection of the peak is not affected by the preceding fluctuation peak and is positive.
I in C! , preparative collection of set peaks, column switching, etc.
A high Hf, low cost preparative separation device that can be automated in one step! Can be provided.

【図面の簡単な説明】[Brief explanation of the drawing]

纂ｌ−框本発明によるピークの検出構成１示すブロック
−１纂２＠α本発明による分取処理のタイムチャート、
富３ｔｌＱＨ設定さ几たピークの検出方法！示した説明
図である。ｌ・・・フィルター、２・一時間設定器。３・−ビータ検出器、ト・・ピーク設定器、５・−増巾
器、６・・・電磁弁。脣許出願人　味の素株式会社代１人　弁履士　佐　藤　英　昭Block 1 shows peak detection configuration according to the present invention.
How to detect peaks with 3tlQH settings! FIG. l... Filter, 2. One hour setting device. 3.-beater detector, peak setting device, 5.-amplifier, 6.-electromagnetic valve. Applicant: Ajinomoto Co., Ltd. Attorney: Hideaki Sato

Claims (1)

Translated fromJapanese

【特許請求の範囲】液体タロマトダラ７！使用した所ｌＩ暇分の分取装置Ｉ
ｃｙて、スター１１表わすイ７ジエクションマーカー信
号【平滑化させるフィルターと１分取設定ピータＯ立上
夕信号に至る間を時間設定により不感帯としてマスタす
る時間設定器と、設定した分取ピークの立上り黴分麦化
ｏｗｌ、ａｓ分を検出するピーク検出器とからなること
に’ｆｌ＃＊とする自動分取装置。[Claims] Liquid Taromatodara 7! I used a preparative separation device I for free time.
cy, A7 extraction marker signal representing star 11 [a time setting device that masters the interval from the smoothing filter to the 1 preparative preparative setting Peter O rise signal as a dead zone by setting the time, and the preparative peak that has been set. An automatic preparative separation device called 'fl#*, which consists of a peak detector that detects the rising owl and as content.