【０００１】[0001]
【産業上の利用分野】本発明は骨を出発原料とするコラ
ーゲン、ヒドロキシアパタイトの製造法及び前記製造法
によって得られる未変性コラーゲン、ヒドロキシアパタ
イトの有効利用に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing collagen and hydroxyapatite using bone as a starting material, and to an effective use of unmodified collagen and hydroxyapatite obtained by the production method.
【０００２】[0002]
【従来の技術】食鳥（ブロイラー）業の大規模化につれ
て大量に排出される鶏の足は、一部が食材として輸出さ
れる以外は、インスタントラーメンのスープ用鳥ガラや
ブロイラー用飼料の原料としてしか使用されていない。
また骨を構成するコラーゲンはゼラチンとして抽出され
るだけで、コラーゲンの製造には牛及び豚の皮が使われ
ているだけである。2. Description of the Related Art Chicken feet, which are discharged in large quantities as the poultry (broiler) business becomes larger, are used as raw materials for instant noodle soup bird gala and broiler feed, except that some are exported as ingredients. Only used as.
Collagen constituting bone is only extracted as gelatin, and only bovine and pig skins are used for collagen production.
【０００３】[0003]
【発明が解決しようとする課題】本発明は、食肉産業の
副産物として容易に入手することができる骨からコラー
ゲン及びヒドロキシアパタイトを抽出し、食肉産業の副
産物を有効利用することを目的としている。SUMMARY OF THE INVENTION An object of the present invention is to extract collagen and hydroxyapatite from bone, which can be easily obtained as a by-product of the meat industry, and to effectively use the by-product of the meat industry.
【０００４】[0004]
【課題を解決するための手段】上記の目的を達成するた
めに、本発明の方法は、皮や身がついたままの鶏等の足
を破砕機で細砕し、塩化ナトリウム、水酸化ナトリウム
水溶液で前処理をした後、種々の濃度の酢酸に浸漬、浸
漬液を中和することで得られる析出物を凍結乾燥するこ
とによって、骨からヒドロキシアパタイトと繊維状の未
変性コラーゲンを得るものである。In order to achieve the above-mentioned object, the method of the present invention comprises the steps of crushing the skin or legs of a chicken or the like with a crusher using a crusher, and adding sodium chloride and sodium hydroxide. After pretreatment with an aqueous solution, immersion in acetic acid of various concentrations, lyophilization of the precipitate obtained by neutralizing the immersion liquid, to obtain hydroxyapatite and fibrous unmodified collagen from bone. is there.
【０００５】[0005]
【作用及び発明の効果】上記製造方法により、前処理で
用いた塩化ナトリウム、水酸化ナトリウムは骨や皮に付
着している血液や脂質、タンパク質に作用し、コラーゲ
ン、ヒドロキシアパタイトを抽出する際の不純物を取り
除くことができる。According to the above-mentioned production method, the sodium chloride and sodium hydroxide used in the pretreatment act on blood, lipids and proteins adhering to bones and skins to extract collagen and hydroxyapatite. Impurities can be removed.
【０００６】次に、コラーゲンとヒドロキシアパタイト
を個々に抽出するために酸の種類や酸濃度について検討
した。その結果、安価な酸の中で、コラーゲンを変性さ
せることなく効率よく抽出するには酢酸がもっとも適当
であった。0.5Nより薄い濃度の酢酸を用いることで、ヒ
ドロキシアパタイトだけを溶出させることができた。な
おヒドロキシアパタイトを溶出させた後、酢酸の濃度を
上げることでコラーゲンだけを抽出することが可能とな
った。Next, in order to extract collagen and hydroxyapatite individually, the type and concentration of acid were examined. As a result, acetic acid was most suitable for efficient extraction of inexpensive acids without denaturing collagen. By using acetic acid at a concentration lower than 0.5 N, only hydroxyapatite could be eluted. After the hydroxyapatite was eluted, it was possible to extract only collagen by increasing the concentration of acetic acid.
【０００７】得られたヒドロキシアパタイトは900 ℃で
１時間加熱しても無煙、無臭で、減量率も公知の方法で
製造する骨灰より少なかった。またこの方法で製造した
骨灰を原料にした骨灰磁器は市販のものより高い白色度
を有していた。The obtained hydroxyapatite was smokeless and odorless even when heated at 900 ° C. for 1 hour, and had a smaller weight loss rate than bone ash produced by a known method. Bone ash porcelain made from bone ash produced by this method had higher whiteness than commercially available ones.
【表１】[Table 1]
【表２】[Table 2]
【０００８】マウスを用いた実験から、従来のものより
骨へのカルシウム吸収性が高かった。[0008] From an experiment using mice, calcium absorption into bone was higher than that of the conventional one.
【０００９】未変性コラーゲンは繊維状のものを得るこ
とができ、パルプとの配合紙への利用が可能となった。
抄紙した配合紙はコラーゲン配合率が高くなるほど吸油
性が向上した。Undenatured collagen can be obtained in a fibrous form, and can be used for paper mixed with pulp.
The oil absorption of the paper-mixed paper was improved as the collagen content increased.
【図１】FIG.
【００１０】[0010]
【実施例１】本発明は下記の実施例によってさらに具体
的に説明されるが、本発明はこれらに限定されるもので
はない。Example 1 The present invention will be described more specifically by the following examples, but the present invention is not limited to these examples.
【００１１】ブロイラーの足を破砕機で細砕し、水で洗
浄し、さらに0.5N以下の濃度の塩化ナトリウム水溶液で
３回以上洗浄後、0.1Nの水酸化ナトリウム水溶液中に懸
濁させ、24時間以上緩やかに撹拌する。The broiler's feet are crushed with a crusher, washed with water, washed three or more times with an aqueous solution of sodium chloride having a concentration of 0.5 N or less, and then suspended in an aqueous solution of 0.1 N sodium hydroxide. Stir gently for at least an hour.
【００１２】次いで固体を0.1Nの酢酸中に懸濁させ、24
時間以上放置する。この物質を5Aのろ紙でろ過、液相を
水酸化ナトリウムで中和してヒドロキシアパタイトを沈
殿させ、蒸留水で洗浄してから回収した。The solid is then suspended in 0.1 N acetic acid and
Leave for more than an hour. This substance was filtered with a 5A filter paper, and the liquid phase was neutralized with sodium hydroxide to precipitate hydroxyapatite, which was recovered after washing with distilled water.
【００１３】再度抽出残渣を0.1N酢酸で抽出し、ヒドロ
キシアパタイトが完全に分離するまで操作を繰り返し
た。The extraction residue was extracted again with 0.1N acetic acid, and the operation was repeated until hydroxyapatite was completely separated.
【００１４】次にヒドロキシアパタイトの抽出残渣を0.
5N以上の酢酸に懸濁させ、24時間以上放置した。この物
質を5Aのろ紙でろ過し、液相を収集し、固体を回収し、
水酸化ナトリウムを前記液相に添加してコラーゲンを析
出させ、これを36/32の透析膜に定量的に移し、蒸留中
で透析を行った。Next, the extraction residue of hydroxyapatite was reduced to 0.
It was suspended in acetic acid of 5N or more and left for 24 hours or more. Filter this material through a 5A filter paper, collect the liquid phase, collect the solid,
Sodium hydroxide was added to the liquid phase to precipitate collagen, which was quantitatively transferred to a 36/32 dialysis membrane and dialyzed during distillation.
【００１５】このコラーゲンをバットに広げ、凍結乾燥
を行い、繊維状のコラーゲンを得た。The collagen was spread on a bat and freeze-dried to obtain fibrous collagen.
【００１６】[0016]
【実施例２】実施例１で得られた沈殿を凍結乾燥し、粉
末状のヒドロキシアパタイトを得た。これを900℃で１
時間加熱して骨灰とし、骨灰、セリサイト粘土、長石、
カオリンを重量比で4:3:2:1の割合で配合し、ポットミ
ルによって湿式混合摩砕、生素地とした。成形後、1200
℃の酸化炎雰囲気で焼成することにより、骨灰磁器素地
を得た。Example 2 The precipitate obtained in Example 1 was freeze-dried to obtain powdery hydroxyapatite. This at 900 ° C
Heat for a time to make bone ash, bone ash, sericite clay, feldspar,
Kaolin was blended at a weight ratio of 4: 3: 2: 1 and wet mixed and ground by a pot mill to obtain a green body. After molding, 1200
By firing in an oxidizing flame atmosphere at ℃, a bone porcelain body was obtained.
【図１】未変性コラーゲンを抄紙した配合紙のコラーゲ
ン配合率による吸油性を示す図である。BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a graph showing oil absorbency according to a collagen blending ratio of a blended paper in which unmodified collagen is made.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17330296AJPH1017310A (en) | 1996-07-03 | 1996-07-03 | Collagen, production of hydroxyapatite and its product |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17330296AJPH1017310A (en) | 1996-07-03 | 1996-07-03 | Collagen, production of hydroxyapatite and its product |
| Publication Number | Publication Date |
|---|---|
| JPH1017310Atrue JPH1017310A (en) | 1998-01-20 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17330296APendingJPH1017310A (en) | 1996-07-03 | 1996-07-03 | Collagen, production of hydroxyapatite and its product |
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