【０００１】[0001]
【産業上の利用分野】本発明は、血小板の凝集能測定方
法及び装置に関し、特に初期凝集及び低濃度凝集惹起剤
による僅かな凝集形成を測定できるようにした血小板凝
集能測定方法及び装置に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method and an apparatus for measuring platelet aggregating ability, and more particularly to a method and an apparatus for measuring platelet aggregating ability capable of measuring initial agglutination and a small amount of agglutination caused by a low concentration agglutinating agent. It is.
【０００２】[0002]
【従来の技術】従来の血小板凝集能測定装置では、血小
板溶液を入れた試料セル中でスターラーバーと呼ばれる
棒状の撹拌部材を通常１０００ｒｐｍで一方向に回転さ
せて血小板溶液を撹拌し、これに光を照射して、その透
過光及び散乱光の強度の変化により血小板凝集能を測定
している。2. Description of the Related Art In a conventional platelet aggregating ability measuring apparatus, a plate-like stirring member called a stirrer bar is normally rotated in one direction at 1000 rpm in a sample cell containing a platelet solution to stir the platelet solution. And the change in the intensity of the transmitted light and the scattered light is used to measure the platelet aggregation ability.
【０００３】[0003]
【発明が解決しようとする課題】ところが、従来の技術
では凝集惹起剤によって発現する血小板の変形と凝集の
両方による光強度変化を測定しており、凝集による変化
のみを測定していない。即ち、血小板の変形による透過
光量の減少と凝集による透過光量の増加の和が測定され
るため、凝集のみを測定することはできない。特に、血
小板の変形は凝集に先だって生じるため、血小板の初期
凝集や低濃度の凝集惹起剤による僅かな凝集変化の測定
は従来技術では不可能である。実際、従来では３０〜４
０％の血小板が凝集してもその変化を捉えることができ
なかった。However, in the prior art, the change in light intensity due to both deformation and aggregation of platelets expressed by the aggregation-inducing agent is measured, and not only the change due to aggregation. That is, since the sum of the decrease in the amount of transmitted light due to the deformation of platelets and the increase in the amount of transmitted light due to aggregation is measured, it is not possible to measure only aggregation. In particular, since the deformation of platelets occurs prior to aggregation, it is impossible with the prior art to measure the initial aggregation of platelets or a slight change in aggregation due to a low concentration of an aggregation inducer. In fact, conventionally, 30 to 4
Even if 0% of platelets aggregated, the change could not be captured.
【０００４】その原因の一つは、従来の試料撹拌方法に
ある。つまり、血小板溶液の撹伴は凝集惹起剤による血
小板凝集を発現させるために不可欠であるが、従来では
円筒ガラスセルにいれたＰＲＰ（Platelet Rich Plasm
a）や洗浄血小板浮遊液などの血小板を含む溶液を撹拌
するために、セル内にスターラーバーを入れ、これを一
方向に通常１０００ｒｐｍで回転させて行っている。One of the causes lies in the conventional sample stirring method. In other words, stirring of the platelet solution is indispensable for expressing platelet aggregation by the aggregation-inducing agent, but conventionally, PRP (Platelet Rich Plasm
In order to stir a solution containing platelets such as a) and washed platelet suspension, a stir bar is inserted into the cell, and the stir bar is rotated in one direction usually at 1000 rpm.
【０００５】ところが、血小板は偏平楕円形の血球であ
るため、その偏平楕円面の向きは撹拌による溶液の流れ
の方向に沿うので、一方向の回転撹拌では血小板が偏平
楕円面を一定方向に向けて撹拌される。このため、血小
板溶液をいれたガラスセルに光を照射し、その透過光量
及び散乱光量を受光して測定する血小板凝集測定では、
偏平楕円形の血小板が変形して球形になると、受光量は
凝集による変化とは反対方向に変化することになる。[0005] However, since platelets are flat elliptical blood cells, the direction of the flat ellipsoid follows the direction of the flow of the solution due to agitation. And stirred. Therefore, in a platelet aggregation measurement in which a glass cell containing a platelet solution is irradiated with light, and the transmitted light amount and the scattered light amount are received and measured.
When the oblate platelet is deformed into a spherical shape, the amount of received light changes in the opposite direction to the change due to aggregation.
【０００６】従って、血小板初期凝集や低濃度凝集惹起
剤による僅かな凝集変化を測定するために受光部の測定
感度を向上させても、真の凝集変化を測定することはで
きない。また、従来技術では回転撹拌時の受光量と撹拌
停止時の受光量が異なることになる。Therefore, even if the measurement sensitivity of the light receiving section is improved to measure the initial aggregation of platelets or the slight aggregation change due to the low concentration aggregation-inducing agent, the true aggregation change cannot be measured. Further, in the related art, the amount of light received when rotating and stirring is different from the amount of light received when stirring is stopped.
【０００７】そこで本発明の課題は、上記のような従来
技術の欠点を解消し、初期凝集及び低濃度凝集惹起剤に
よる僅かな凝集形成を正確に測定できる血小板凝集能測
定方法及び装置を提供することにある。[0007] Therefore, an object of the present invention is to provide a method and an apparatus for measuring platelet aggregation ability, which can solve the above-mentioned disadvantages of the prior art and can accurately measure initial aggregation and slight aggregation formation caused by a low-concentration aggregation-inducing agent. It is in.
【０００８】[0008]
【課題を解決するための手段】上記の課題を解決するた
め、本発明によれば、血小板溶液を撹拌し、該溶液に光
を照射して透過光及び散乱光の強度変化により血小板凝
集能を測定する血小板凝集能測定方法において、前記血
小板溶液の撹拌は、該溶液中の血小板の偏平楕円面の向
きが不定になるように行なう方法を採用した。According to the present invention, a platelet solution is agitated, and the solution is irradiated with light to change the intensity of transmitted light and scattered light. In the method for measuring platelet aggregation ability to be measured, a method was employed in which the platelet solution was stirred such that the direction of the oblate ellipsoid of platelets in the solution was indefinite.
【０００９】また本発明によれば、血小板溶液とともに
磁性体からなる撹拌部材を入れた試料セルの近傍でマグ
ネットを駆動することにより、前記撹拌部材を動かして
血小板溶液を撹拌し、該溶液に光を照射して透過光及び
散乱光の強度変化により血小板凝集能を測定する血小板
凝集能測定装置において、前記マグネットを駆動する駆
動手段は前記マグネットを交互に正逆方向に所定角度ず
つ回転駆動する、若しくは前記マグネットを振動させる
ように構成した。Further, according to the present invention, the platelet solution is stirred by moving the stirring member by driving the magnet near the sample cell containing the stirring member made of a magnetic material together with the platelet solution. In the platelet aggregating ability measuring apparatus that measures platelet aggregating ability by changing the intensity of transmitted light and scattered light by irradiating the magnet, the driving means for driving the magnet alternately rotates the magnet by a predetermined angle in forward and reverse directions. Alternatively, the magnet is configured to vibrate.
【００１０】[0010]
【作用】上記の本発明の方法によれば、血小板溶液の撹
拌による血小板の偏平楕円面の向きが不定であるため、
前述した血小板の変形があってもそれによる透過光及び
散乱光の強度変化はない。According to the method of the present invention, the direction of the oblate ellipsoid of the platelets due to the stirring of the platelet solution is indefinite.
Even if the platelets are deformed as described above, there is no change in the intensity of the transmitted light and the scattered light.
【００１１】また上記の本発明の装置によれば、上記撹
拌部材を交互に正逆方向に回転駆動する、若しくは振動
させて血小板溶液を撹拌することになるので、その撹拌
による血小板の溶液の流れの向きは不定、ランダムであ
り、前記流れによる血小板の偏平楕円面の向きも不定に
なる。According to the apparatus of the present invention, the platelet solution is agitated by alternately rotating or agitating the agitating member in the forward / reverse direction, thereby stirring the platelet solution. Is indeterminate and random, and the direction of the flat ellipsoid of the platelets due to the flow is also indeterminate.
【００１２】[0012]
【実施例】以下、図を参照して本発明の実施例を説明す
る。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings.
【００１３】［第１実施例］図１は本発明の第１実施例
による血小板凝集能測定装置の要部の構成を示してい
る。[First Embodiment] FIG. 1 shows the configuration of a main part of a platelet aggregation measuring apparatus according to a first embodiment of the present invention.
【００１４】図１に示すように、装置のホルダ部２０に
形成された穴２０ａ内に、血小板溶液７をいれた透明な
試料セル３がセットされる。試料セル３はガラスから円
筒形に形成されており、その底に磁性体からなる細棒状
の撹拌部材であるスターラーバー４が水平に置かれてい
る。穴２０ａの両側には、血小板溶液７に光を照射する
光源５と、その照射光の溶液７を透過する透過光および
散乱光を受光する受光素子６が配置されている。また、
ホルダ部２０の下方にはＤＣモータ１が設けられてお
り、その回転軸にはマグネット２が固着され、穴２０ａ
の真下に配置されている。As shown in FIG. 1, a transparent sample cell 3 containing a platelet solution 7 is set in a hole 20a formed in a holder section 20 of the apparatus. The sample cell 3 is formed in a cylindrical shape from glass, and a stirrer bar 4 as a thin rod-shaped stirring member made of a magnetic material is placed horizontally at the bottom. On both sides of the hole 20a, a light source 5 for irradiating the platelet solution 7 with light, and a light receiving element 6 for receiving transmitted light and scattered light of the irradiation light passing through the solution 7 are arranged. Also,
A DC motor 1 is provided below the holder 20. A magnet 2 is fixed to a rotation shaft of the DC motor 1, and a hole 20a
It is located directly below.
【００１５】次に図１の構成による測定動作を説明す
る。測定時には、符号８で示す正負のモータ駆動パルス
によりＤＣモータ１を周期的に交互に正逆の両方向に所
定角度ずつ回転駆動する。これによりマグネット２が交
互に正逆方向に所定角度ずつ回転され、その磁力により
スターラーバー４が矢印のように両方向に交互に回転
し、血小板溶液７を撹拌する。この撹拌による血小板溶
液７の流れはランダムつまり不定なものとなる。Next, a description will be given of a measuring operation by the configuration of FIG. At the time of measurement, the DC motor 1 is periodically and alternately rotated by a predetermined angle in both forward and reverse directions by positive and negative motor drive pulses indicated by reference numeral 8. As a result, the magnets 2 are alternately rotated in the forward and reverse directions by a predetermined angle, and the magnetic force causes the stirrer bar 4 to alternately rotate in both directions as indicated by arrows, thereby stirring the platelet solution 7. The flow of the platelet solution 7 due to this stirring becomes random, that is, indefinite.
【００１６】そして光源５を点灯し、その照射光の血小
板溶液７を透過する透過光および散乱光を受光素子６で
受光してその強度を検出し、その強度変化により血小板
凝集能を測定する。Then, the light source 5 is turned on, and the transmitted light and the scattered light of the irradiation light transmitted through the platelet solution 7 are received by the light receiving element 6 to detect the intensity, and the platelet aggregation ability is measured by the change in the intensity.
【００１７】ここで撹拌条件としては、（１）撹拌時と
撹拌停止時とで透過光、散乱光強度に差がなく、（２）
最大凝集を引き起こす濃度の凝集惹起剤、例えば、ＡＤ
Ｐ（adenosin diphosphate）を２〜４マイクロモル添加
して３〜５分後に８０〜９０％の最大凝集が発現し、
（３）従来法と同じ凝集曲線が得られる、条件を選択す
る。Here, the stirring conditions include (1) no difference in transmitted light and scattered light intensity between the time of stirring and the time of stopping stirring, and (2)
A concentration of an aggregation-inducing agent such as AD
After 3 to 5 minutes from the addition of 2 to 4 micromoles of P (adenosin diphosphate), 80 to 90% of the maximum aggregation appears,
(3) Select the conditions that give the same aggregation curve as in the conventional method.
【００１８】即ち、このような撹拌条件を満たすため
に、試料セル３の大きさ、血小板溶液７の量、スターラ
ーバー４の大きさ、撹拌の回転角度および周期を選択す
る。That is, in order to satisfy such stirring conditions, the size of the sample cell 3, the amount of the platelet solution 7, the size of the stirrer bar 4, the rotation angle and cycle of the stirring are selected.
【００１９】例えば、内径７×高さ５０ｍｍの試料セル
に３００μｌの血小板溶液（血小板数：１０の８乗個／
ｍｌ）を入れ、外径１×長さ５ｍｍのスターラーバーを
用いて、撹拌の回転角度８５度、周期２０Ｈｚで測定実
験を行なった。それによる測定例と従来装置で一方向回
転撹拌（１０００ｒｐｍ）による測定例の結果を図２、
図３に示す。なお、これはウサギから採取した血小板の
凝集の測定例であり、図２は撹拌方法の差異による血小
板の偏平楕円面と変形の透過光（及び散乱光）強度に対
する影響を示し、図３は凝集惹起剤トロンビンによる血
小板凝集の透過光（及び散乱光）強度の変化を示してい
る。なお両図中で（Ａ−１）〜（Ａ−４）は従来装置の
測定例、（Ｂ−１）〜（Ｂ−４）は本実施例の測定例を
示す。For example, in a sample cell having an inner diameter of 7 × 50 mm in height, 300 μl of a platelet solution (the number of platelets: 10 to the eighth power /
ml), and a measurement experiment was carried out using a stirrer bar having an outer diameter of 1 × 5 mm in length at a rotation angle of stirring of 85 ° and a cycle of 20 Hz. FIG. 2 shows the results of the measurement example using the conventional apparatus and the measurement example using one-way rotation stirring (1000 rpm) in the conventional apparatus.
As shown in FIG. This is an example of measurement of the aggregation of platelets collected from rabbits. FIG. 2 shows the flat ellipsoid of platelets and the effect of the deformation on the transmitted light (and scattered light) intensity due to the difference in the agitation method, and FIG. The change of the transmitted light (and scattered light) intensity of platelet aggregation by the inducer thrombin is shown. In both figures, (A-1) to (A-4) show measurement examples of the conventional apparatus, and (B-1) to (B-4) show measurement examples of the present embodiment.
【００２０】図２の（Ａ−１）から明らかなように、従
来装置の測定例において、透過光強度は撹拌開始により
上昇して撹拌停止により減少し、血小板の偏平楕円面が
一定の向きを持って撹拌されていることがわかる。また
（Ａ−２）に示すように、ＥＧＴＡを含む無Ｃａ2+液中
で凝集が生じないようにしてトロンビンを０．３ｕｎｉ
ｔ／ｍｌ添加すると、透過光量は減少し、血小板の変形
による透過光量変化が測定されていることがわかる。As apparent from (A-1) of FIG. 2, in the measurement example of the conventional apparatus, the transmitted light intensity increases when the stirring is started and decreases when the stirring is stopped, and the flat ellipsoid of the platelets is oriented in a certain direction. It turns out that it is being stirred and held. In addition, as shown in (A-2), thrombin was added to 0.3 uni so as to prevent aggregation in a Ca2+ -free solution containing EGTA.
It can be seen that the addition of t / ml decreases the amount of transmitted light, and the change in the amount of transmitted light due to the deformation of platelets is measured.
【００２１】一方、（Ｂ−１）に示すように、本実施例
の測定例では、撹拌の開始と停止による透過光量の変化
はなく、血小板の偏平楕円面の向きが不定、ランダムな
状態で撹拌されていることがわかる。また（Ｂ−２）に
示すように、ＥＧＴＡを含む無Ｃａ2+液中でトロンビン
を作用させてもその前後で透過光量に変化はなく、血小
板の変形による透過光量変化が測定されていないことが
わかり、血小板の凝集による光量変化のみ測定されるこ
とが確認される。On the other hand, as shown in (B-1), in the measurement example of this embodiment, there is no change in the amount of transmitted light due to the start and stop of the stirring, and the orientation of the oblate ellipsoid of the platelets is indeterminate and random. It turns out that it is stirring. Further, as shown in (B-2), even when thrombin is acted on in a Ca2+ -free solution containing EGTA, the transmitted light amount does not change before and after that, and the change in transmitted light amount due to platelet deformation is not measured. It is confirmed that only a change in light amount due to platelet aggregation is measured.
【００２２】また図３の（Ａ−３）に示すように、従来
装置ではトロンビン０．３ｕｎｉｔ／ｍｌの添加により
透過光量は減少した後に増加し、血小板の変形と凝集に
よる変化が重なって測定されていることがわかる。In addition, as shown in FIG. 3A-3, in the conventional apparatus, the amount of transmitted light decreases after addition of 0.3 unit / ml of thrombin and then increases. You can see that it is.
【００２３】これに対し（Ｂ−３）に示すように、本実
施例では、血小板凝集による凝集塊発現に伴う透過光量
変化のみ測定され、且つ、従来法と同じ凝集曲線が得ら
れることがわかる。On the other hand, as shown in (B-3), in this example, only the change in the amount of transmitted light due to the appearance of aggregates due to platelet aggregation was measured, and the same aggregation curve as that of the conventional method was obtained. .
【００２４】よって、本実施例の測定装置によれば、初
期凝集発現までの遅延時間及び凝集発現の初期速度が正
確に測定でき、従来技術で測定される最大凝集率と最大
凝集速度を含めた多くの測定項目の測定ができるように
なる。Thus, according to the measuring apparatus of the present embodiment, the delay time until the onset of initial agglutination and the initial rate of the onset of agglutination can be accurately measured. Many measurement items can be measured.
【００２５】また図３の（Ａ−４）に示すように、低濃
度トロンビン０．０１ｕｎｉｔ／ｍｌの添加により、光
学顕微鏡の観察では凝集が生じているにもかかわらず、
従来装置では透過光量の増加は観察されず、むしろ血小
板の変形による透過光量の減少が見られる。As shown in FIG. 3 (A-4), the addition of 0.01 unit / ml of low-concentration thrombin, although observed under an optical microscope, has caused aggregation.
In the conventional apparatus, an increase in the amount of transmitted light is not observed, but rather, a decrease in the amount of transmitted light due to deformation of platelets is observed.
【００２６】これに対し、（Ｂ−４）に示すように、本
実施例装置では低濃度トロンビン０．０１ｕｎｉｔ／ｍ
ｌの添加で発現する凝集による透過光量の増加が観察さ
れ、従来技術では測定できなかった僅かな凝集塊の形成
による変化を測定できる。このように、より低濃度の凝
集惹起剤による凝集を測定できることにより、凝集を起
こし易い血小板の動態を知ることができ、臨床における
血栓症などの診断や治療効果の判定に役立てることがで
きる。On the other hand, as shown in (B-4), the apparatus of this embodiment has a low concentration of thrombin of 0.01 unit / m 2.
An increase in the amount of transmitted light due to aggregation caused by the addition of 1 is observed, and a change due to the formation of a slight aggregate that cannot be measured by the conventional technique can be measured. As described above, by measuring the aggregation caused by the lower concentration of the aggregation inducing agent, it is possible to know the dynamics of platelets that are likely to cause aggregation, which can be useful for diagnosing thrombosis and the like and determining the therapeutic effect in clinical practice.
【００２７】［第２実施例］次に、図４は本発明の第２
実施例による血小板凝集能測定装置の要部の構成を示し
ている。[Second Embodiment] Next, FIG. 4 shows a second embodiment of the present invention.
1 shows a configuration of a main part of a platelet aggregation measuring apparatus according to an embodiment.
【００２８】本実施例では試料セル３中のスターラーバ
ー４を駆動するための構成が第１実施例と異なってお
り、試料セル３を保持するホルダ部２０の直下にマグネ
ット１１がコイルバネ１２により弾性的に可動に支持さ
れており、その下方にコイル９を鉄芯１０に巻回してな
る電磁石が配置されている。In this embodiment, the structure for driving the stirrer bar 4 in the sample cell 3 is different from that of the first embodiment. The magnet 11 is elastically provided by the coil spring 12 directly below the holder 20 for holding the sample cell 3. An electromagnet formed by winding a coil 9 around an iron core 10 is disposed below the electromagnet.
【００２９】このような構成により、測定時には、コイ
ル９に所定周波数のＡＣ電圧を印加してマグネット１１
を振動させ、その磁力によりスターラーバー４を振動さ
せて血小板溶液７を撹拌させる。そして第１実施例と同
様に光源５を点灯し、その照射光の血小板溶液７を透過
する透過光および散乱光を受光素子６で受光してその強
度を検出し、その強度変化により血小板凝集能を測定す
る。With this configuration, at the time of measurement, an AC voltage having a predetermined frequency is
And the stirrer bar 4 is vibrated by the magnetic force to stir the platelet solution 7. The light source 5 is turned on in the same manner as in the first embodiment, and the transmitted light and the scattered light of the irradiation light transmitted through the platelet solution 7 are received by the light receiving element 6 to detect the intensity. Is measured.
【００３０】この場合、スターラーバー４の振動により
撹拌される血小板溶液７の流れはランダムであって血小
板溶液７中の血小板の偏平楕円面の向きはランダム、不
定となり、第１実施例と同様の作用効果が得られる。In this case, the flow of the platelet solution 7 stirred by the vibration of the stirrer bar 4 is random, and the direction of the flat ellipsoid of the platelets in the platelet solution 7 is random and undefined. An effect can be obtained.
【００３１】[0031]
【発明の効果】以上の説明から明らかなように、本発明
によれば、血小板溶液を撹拌し、該溶液に光を照射して
透過光及び散乱光の強度変化により血小板凝集能を測定
する血小板凝集能測定方法及び装置において、前記血小
板溶液の撹拌は、該溶液中の血小板の偏平楕円面の向き
が不定になるように行なうので、血小板凝集に伴なう血
小板の変形による透過光及び散乱光の強度変化を除き、
凝集塊形成に伴う変化のみ測定でき、特に、血小板の変
形が発現するところの初期凝集変化や低濃度凝集惹起剤
による僅かな凝集形成変化を正確に測定でき、臨床検査
における血小板機能測定や抗血小板薬の検定などに極め
て有用であるという優れた効果が得られる。As is apparent from the above description, according to the present invention, a platelet solution is stirred by stirring a platelet solution, irradiating the solution with light, and measuring the platelet aggregation ability by the intensity change of transmitted light and scattered light. In the method and apparatus for measuring agglutinability, the stirring of the platelet solution is performed so that the direction of the oblate ellipsoid of the platelets in the solution is indefinite, so that the transmitted light and the scattered light due to the deformation of the platelets accompanying the platelet aggregation Except for the intensity change of
Only changes associated with aggregate formation can be measured, especially initial aggregation changes where platelet deformation occurs and slight changes in aggregate formation due to low-concentration aggregation inducers can be accurately measured. An excellent effect is obtained that is extremely useful for drug testing and the like.
【図１】本発明の第１実施例による血小板凝集能測定装
置の要部の機械的構成と動作を示す斜視図である。FIG. 1 is a perspective view showing a mechanical configuration and operation of a main part of a platelet aggregation measuring apparatus according to a first embodiment of the present invention.
【図２】実施例の測定装置と従来装置の撹拌方法の差異
による血小板の偏平楕円面の向きと変形の透過光（及び
散乱光）強度に対する影響を示した線図である。FIG. 2 is a diagram showing the influence on the transmitted light (and scattered light) intensity of the direction of the oblate ellipsoid of platelets and the deformation due to the difference in the stirring method between the measuring apparatus of the embodiment and the conventional apparatus.
【図３】実施例の装置と従来装置の測定例における凝集
惹起剤トロンビンによる血小板凝集の透過光（及び散乱
光）強度の変化を示した線図である。FIG. 3 is a diagram showing a change in transmitted light (and scattered light) intensity of platelet aggregation by the aggregation-inducing agent thrombin in the apparatus of the example and the measurement example of the conventional apparatus.
【図４】第２実施例による血小板凝集能測定装置の要部
の機械的構成と動作を示す説明図である。FIG. 4 is an explanatory diagram showing a mechanical configuration and operation of a main part of a platelet aggregation measuring apparatus according to a second embodiment.
１ ＤＣモータ ２ マグネット ３ 試料セル ４ スターラーバー ５ 光源 ６ 受光素子 ７ 血小板溶液 ９ コイル １０ 鉄芯 １１ マグネット １２ コイルばね Reference Signs List 1 DC motor 2 Magnet 3 Sample cell 4 Stirrer bar 5 Light source 6 Light receiving element 7 Platelet solution 9 Coil 10 Iron core 11 Magnet 12 Coil spring
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 昭63−12962（ＪＰ，Ａ) 特開 昭58−187859（ＪＰ，Ａ) 特開 昭61−116658（ＪＰ，Ａ) 特開 昭60−113153（ＪＰ，Ａ) 実開 昭56−43052（ＪＰ，Ｕ) 実開 昭52−43053（ＪＰ，Ｕ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 33/48 - 33/52 G01N 33/58 - 33/98──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-63-12962 (JP, A) JP-A-58-187859 (JP, A) JP-A-61-116658 (JP, A) JP-A-60-160 113153 (JP, A) Japanese Utility Model Showa 56-43052 (JP, U) Japanese Utility Model Showa 52-43053 (JP, U) (58) Fields investigated (Int. Cl.7 , DB name) G01N 33/48-33 / 52 G01N 33/58-33/98
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP04041769AJP3137713B2 (en) | 1992-02-28 | 1992-02-28 | Platelet aggregation measuring method and apparatus |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP04041769AJP3137713B2 (en) | 1992-02-28 | 1992-02-28 | Platelet aggregation measuring method and apparatus |
| Publication Number | Publication Date |
|---|---|
| JPH05240863A JPH05240863A (en) | 1993-09-21 |
| JP3137713B2true JP3137713B2 (en) | 2001-02-26 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP04041769AExpired - Fee RelatedJP3137713B2 (en) | 1992-02-28 | 1992-02-28 | Platelet aggregation measuring method and apparatus |
| Country | Link |
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| JP (1) | JP3137713B2 (en) |
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