本発明は育毛促進技術に関する。詳細には、本発明はオゾンマイクロバブルシャワーを皮膚に適用することによる育毛技術および皮膚活性化技術に関する。 The present invention relates to a hair growth promoting technique. Specifically, the present invention relates to a hair growth technique and a skin activation technique by applying an ozone microbubble shower to the skin.
頭髪の育毛は多くの人に望まれており、これまで様々な育毛技術が開発されてきた。これまでの育毛技術は、育毛剤（特許文献１）を皮膚や毛根に適用するものが主流であるが、効果が十分でない場合が多く、適用に際し不快感がある、副作用がある、効能に男女差がある等の問題を有していた。また、生活習慣を改善するアプローチ、具体的には食事療法や精神的な要因を除去するといったアプローチ、あるいはホルモンバランスを変化させるアプローチもあるが、やはり効果が十分でない、面倒である、副作用がある等の問題があった。 Hair growth is desired by many people, and various hair growth techniques have been developed so far. Up until now, hair growth technology (Patent Document 1) is mainly applied to the skin and hair roots, but the effect is often not sufficient, there are unpleasantness in application, there are side effects, and men and women are effective. There were problems such as differences. There are also approaches to improve lifestyle habits, specifically to remove dietary and mental factors, or approaches to change hormone balance, but they are still not effective, tedious, and have side effects There was a problem such as.
本発明の課題は、十分な効果を有し、使用に際して不快感がなく、副作用がなく、しかも効能に男女差がない育毛技術および皮膚活性化技術を開発することであった。 The object of the present invention was to develop a hair-growth technique and a skin activation technique that have sufficient effects, no discomfort during use, no side effects, and no gender differences in efficacy.
本発明者らは上記課題を解決せんと鋭意研究を重ね、オゾンマイクロバブルシャワーを皮膚に適用することにより、爽快な使用感を伴って育毛効果と皮膚活性化効果が得られ、副作用も認められないこと、効果に男女差がないこと、オゾンマイクロバブルシャワーにミネラル類を含有させるとさらに育毛効果および皮膚活性化効果が高まること等を見出し、本発明を完成させるに至った。 The present inventors have intensively studied to solve the above problems, and by applying ozone micro bubble shower to the skin, a hair-growth effect and a skin activation effect are obtained with a refreshing feeling of use, and side effects are also recognized. It has been found that there is no difference between men and women in the effect, and that minerals are added to the ozone microbubble shower, the hair-growth effect and skin activation effect are further enhanced, and the present invention has been completed.
すなわち、本発明は以下のものを提供する：
（１）毛髪の育毛に用いられる、オゾンバブルを含む水性組成物。
（２）ミネラル類をさらに含む（１）記載の組成物。
（３）毛髪が頭髪である（１）または（２）に記載の組成物。
（４）（１）〜（３）のいずれかに記載の組成物を動物（ヒトを除く）の皮膚に適用することを特徴とする、毛髪の育毛方法。
（５）皮膚の活性化に用いられる、オゾンバブルを含む水性組成物。
（６）ミネラル類をさらに含む（５）記載の組成物。
（７）皮膚が頭皮である（５）または（６）に記載の組成物。
（８）（５）〜（７）のいずれかに記載の組成物を動物（ヒトを除く）の皮膚に適用することを特徴とする、皮膚の活性化方法。That is, the present invention provides the following:
(1) An aqueous composition containing ozone bubbles used for hair growth.
(2) The composition according to (1), further comprising minerals.
(3) The composition according to (1) or (2), wherein the hair is scalp hair.
(4) A method for growing hair, comprising applying the composition according to any one of (1) to (3) to the skin of an animal (excluding humans).
(5) An aqueous composition containing ozone bubbles used for skin activation.
(6) The composition according to (5), further comprising minerals.
(7) The composition according to (5) or (6), wherein the skin is a scalp.
(8) A method for activating skin, comprising applying the composition according to any one of (5) to (7) to the skin of an animal (excluding humans).
本発明によれば、十分な効果を有し、使用に際して不快感がなく、副作用もなく、効能に男女差もない育毛技術が提供される。 ADVANTAGE OF THE INVENTION According to this invention, the hair-growth technique which has sufficient effect, there is no discomfort at the time of use, there is no side effect, and there is no gender difference in effect is provided.
本発明は、１の態様において、毛髪の育毛に用いられる、オゾンバブルを含む水性組成物を提供する。本発明による育毛効果は、育毛が望まれる部位の皮膚にオゾンバブルを含む水性組成物を適用することによって達成される。本発明のオゾンマイクロバブルシャワーは、オゾンの有する抗菌効果とマイクロバブルが有する皮膚へのキャビテーション効果による皮膚活性化効果が相俟って、すぐれた育毛効果が得られる。育毛が望まれる部位はいずれの部位であってもよいが、好ましくは頭皮に適用して毛髪の育毛を促進する。本発明のオゾンバブルを含む水性組成物は、オゾンバブルを含む水であってもよく、あるいはオゾンバブルを含む水および他の水溶性物質の混合物であればよい。水溶性物質の例としてはアルコール類などが挙げられる。本発明の水性組成物は、ミノキシジル、アデノシン、センブリエキスなどの公知の育毛成分、ミネラル類、ビタミン類、シャンプー、リンス、香料、整髪料、化粧料などを含んでいてもよい。 In one aspect, the present invention provides an aqueous composition containing ozone bubbles for use in hair growth. The hair growth effect according to the present invention is achieved by applying an aqueous composition containing ozone bubbles to the skin where hair growth is desired. The ozone microbubble shower of the present invention combines the antibacterial effect of ozone and the skin activation effect by the cavitation effect on the skin that microbubbles have, and provides an excellent hair growth effect. The site where hair growth is desired may be any site, but is preferably applied to the scalp to promote hair growth. The aqueous composition containing ozone bubbles of the present invention may be water containing ozone bubbles, or may be a mixture of water containing ozone bubbles and other water-soluble substances. Examples of water-soluble substances include alcohols. The aqueous composition of the present invention may contain known hair-growth ingredients such as minoxidil, adenosine and assembly extract, minerals, vitamins, shampoos, rinses, fragrances, hair conditioners, cosmetics and the like.
本発明のオゾンバブルを含む水性組成物の皮膚への適用は、本発明の水性組成物が、育毛を要する動物の皮膚部位と接触するような態様で行えばよく、特に制限はないが、本発明の水性組成物をシャワー状にして適用することが好ましい。本発明の水性組成物をシャワー状として皮膚に適用する場合の適用圧力および水流量は、皮膚や頭皮へのダメージが生じず、使用者が不快に感じない範囲内で適宜増減することができる。通常は、上記範囲内であれば、圧力は強いほうが好ましく、水流も多い方が好ましい。本発明の水性組成物の皮膚への１回あたりの適用時間も動物の種類、皮膚の状態、発毛の状態などを考慮して適宜調節でき、数十秒、数分あるいは数十分であってもよい。例えば、ヒト、イヌ、ネコなどの場合には１回あたり３０秒〜３０分適用することができ、例えば、１回あたり３０秒〜６０秒、１分〜３分、３分〜５分、５分〜７分、７分〜１０分、１０分〜１５分、１５分〜２０分適用してもよい。本発明の水性組成物の適用回数も同様に適宜調節でき、１日１回〜数回、例えば、ヒト、イヌ、ネコなどの場合には１日１回、２回、３回、４回、５回あるいは６回適用してもよい。上記処置を所望の効果が現れるまで継続することができる。通常は数週間〜数ヶ月、例えば１〜２週間ないし１〜２ヶ月で効果が見られる。効果が現れた後も本発明の水性組成物を適用し、効果を維持することができる。 The application of the aqueous composition containing the ozone bubble of the present invention to the skin is not particularly limited as long as the aqueous composition of the present invention is in contact with the skin site of an animal that requires hair growth. It is preferable to apply the aqueous composition of the invention in the form of a shower. When the aqueous composition of the present invention is applied to the skin in the form of a shower, the applied pressure and the water flow rate can be appropriately increased or decreased within the range where the skin and scalp are not damaged and the user does not feel uncomfortable. Usually, if it is in the said range, the one where a pressure is stronger is preferable and a water flow is also more preferable. The application time of the aqueous composition of the present invention to the skin can also be adjusted as appropriate in consideration of the type of animal, skin condition, hair growth condition, etc., and it can be several tens of seconds, several minutes or several tens of minutes. May be. For example, in the case of humans, dogs, cats, etc., it can be applied for 30 seconds to 30 minutes at a time, for example, 30 seconds to 60 seconds, 1 minute to 3 minutes, 3 minutes to 5 minutes, 5 times, Minutes to 7 minutes, 7 minutes to 10 minutes, 10 minutes to 15 minutes, 15 minutes to 20 minutes may be applied. The frequency of application of the aqueous composition of the present invention can be adjusted as appropriate, once to several times a day, for example, once a day, two times, three times, four times in the case of humans, dogs, cats, etc. You may apply 5 times or 6 times. The treatment can be continued until the desired effect appears. Usually, an effect is seen in several weeks to several months, for example, 1-2 weeks to 1-2 months. Even after the effect appears, the aqueous composition of the present invention can be applied to maintain the effect.
本発明の水性組成物中のオゾンバブルの数は多いほど好ましく、バブルのサイズは小さいほど好ましい。その理由は、細かく、しかも大量のオゾンバブルを皮膚に適用すると、バブルが毛穴の奥まで到達し、より多くの老廃物を除去し、加えてオゾンによる殺菌効果も強くなるからである。本発明の水性組成物中のオゾンバブルは通常、オゾンガス流量約１．０〜１．５Ｌ／ｍｉｎ以上であることが好ましく、好ましくは１．５〜２．０Ｌ／ｍｉｎ以上である。 The greater the number of ozone bubbles in the aqueous composition of the present invention, the better. The smaller the bubble size, the better. The reason is that if fine ozone bubbles are applied to the skin, the bubbles reach the back of the pores to remove more waste products, and in addition, the bactericidal effect by ozone becomes stronger. In general, the ozone bubbles in the aqueous composition of the present invention preferably have an ozone gas flow rate of about 1.0 to 1.5 L / min or more, preferably 1.5 to 2.0 L / min or more.
本発明の水性組成物の温度は特に限定はないが、動物が不快に感じない温度範囲が好ましい。例えば、２５〜４２℃、３０〜３３℃、３３〜３６℃、３６〜３９℃、３９〜４２℃などであってもよい。 The temperature of the aqueous composition of the present invention is not particularly limited, but a temperature range in which animals do not feel uncomfortable is preferable. For example, 25-42 degreeC, 30-33 degreeC, 33-36 degreeC, 36-39 degreeC, 39-42 degreeC, etc. may be sufficient.
水性組成物中にオゾンバブルを存在せしめる手段・方法は公知であり、オゾンガスをバブリングする方式やエゼクター方式などを用いることができる。 Means and methods for causing ozone bubbles to exist in the aqueous composition are known, and a method of bubbling ozone gas, an ejector method, or the like can be used.
本発明のオゾンバブルを含む水性組成物とミネラル類とを併用して皮膚に適用した場合に、さらなる育毛効果が得られる。好ましくは、本発明のオゾンバブルを含む水性組成物にミネラル類を含有させて皮膚に適用する。したがって、本発明は、ミネラル類をさらに含む、オゾンバブルを含む水性組成物を提供する。しかし、本発明のオゾンバブルを含む水性組成物とミネラル類とを一緒に皮膚に適用する必要はない。本発明のオゾンバブルを含む水性組成物の適用前、後、あるいは本発明のオゾンバブルを含む水性組成物の適用の合間にミネラル類を皮膚に適用してもよい。 When the aqueous composition containing the ozone bubble of the present invention and the minerals are used in combination and applied to the skin, a further hair-growth effect is obtained. Preferably, minerals are contained in the aqueous composition containing the ozone bubble of the present invention and applied to the skin. Accordingly, the present invention provides an aqueous composition comprising ozone bubbles further comprising minerals. However, it is not necessary to apply the aqueous composition containing the ozone bubble of the present invention and minerals together to the skin. Minerals may be applied to the skin before, after or after application of the aqueous composition containing ozone bubbles of the present invention, or between application of the aqueous composition containing ozone bubbles of the present invention.
ミネラル類とは、地球上に存在する元素のうち、一般的な有機物に含まれる水素、炭素、窒素、酸素を除いたものをいい、典型的なものとして、ナトリウム、マグネシウム、リン、イオウ、塩素、カリウム、カルシウム、クロム、マンガン、鉄、コバルト、銅、亜鉛、セレン、モリブデン、ヨウ素などが挙げられる。本明細書において「ミネラル類」という場合には、上記元素、それらのイオン形態、それらを含む塩をも包含するものとする。一般的には、ミネラル類は塩の形で用いられる。ミネラル類が本発明のオゾンバブルを含む水性組成物に塩の形で含まれていてもよい。 Minerals are the elements existing on the earth, excluding hydrogen, carbon, nitrogen and oxygen contained in general organic matter. Typical examples are sodium, magnesium, phosphorus, sulfur and chlorine. , Potassium, calcium, chromium, manganese, iron, cobalt, copper, zinc, selenium, molybdenum, iodine and the like. In the present specification, the term “minerals” includes the above elements, their ionic forms, and salts containing them. In general, minerals are used in the form of salts. Minerals may be contained in the form of a salt in the aqueous composition containing the ozone bubble of the present invention.
本発明のオゾンバブルを含む水性組成物に添加される好ましいミネラル類としては、ナトリウム、カリウム、リン、マグネシウム、カルシウム、硫黄、塩素、クロム、モリブデン、マンガン、鉄、銅、亜鉛、セレニウム、ヨウ素、コバルトなどが挙げられる。より好ましいミネラル類としては亜鉛、マグネシウム、ヨウ素、鉄、硫黄などが挙げられ、特に好ましいミネラル類としては亜鉛、ヨウ素、鉄、硫黄などが挙げられる。本発明の水性組成物中のミネラル類の濃度も、ミネラルの種類、溶解度、べとつき感や効果を考慮しながら適宜調節できる。 Preferred minerals added to the aqueous composition containing the ozone bubble of the present invention include sodium, potassium, phosphorus, magnesium, calcium, sulfur, chlorine, chromium, molybdenum, manganese, iron, copper, zinc, selenium, iodine, Examples include cobalt. More preferable minerals include zinc, magnesium, iodine, iron, sulfur and the like, and particularly preferable minerals include zinc, iodine, iron, sulfur and the like. The concentration of minerals in the aqueous composition of the present invention can also be appropriately adjusted in consideration of the type of mineral, solubility, stickiness and effects.
本発明は、別の態様において、上記水性組成物を動物の皮膚に適用することを特徴とする、毛髪の育毛方法を提供する。動物はヒトであってもよく、ヒト以外の動物であってもよい。例えば、イヌ、ネコなどのペット動物に本発明の方法を適用することができる。 In another aspect, the present invention provides a method for hair growth, characterized in that the aqueous composition is applied to the skin of an animal. The animal may be a human or a non-human animal. For example, the method of the present invention can be applied to pet animals such as dogs and cats.
さらに本発明は、育毛を要する動物の皮膚部位において育毛を促進させるための、上記水性組成物の使用を提供する。 Furthermore, this invention provides use of the said aqueous composition for promoting hair growth in the skin site | part of the animal which requires hair growth.
本発明のオゾンマイクロバブルシャワーは、オゾンの有する抗菌効果とマイクロバブルが有する皮膚へのキャビテーション効果による皮膚活性化効果を有する。したがって、本発明は、さらなる態様において、育毛促進のほかに皮膚の活性化にも効果的である。したがって、本発明は、さらなる態様において、皮膚の活性化に用いられる、オゾンバブルを含む水性組成物を提供する。皮膚の活性化を目的とする場合にも、上記の育毛効果に関する説明があてはまる。当該皮膚の活性化には、表皮細胞の活性化、毛母細胞の活性化など、皮膚表層およびその周辺に存在する細胞の活性化が含まれる。特に、本発明のオゾンマイクロバブルシャワーは頭皮への適用に適する。 The ozone microbubble shower of the present invention has an antibacterial effect that ozone has and a skin activation effect due to the cavitation effect on the skin that microbubbles have. Therefore, in a further aspect, the present invention is effective for activating the skin in addition to promoting hair growth. The invention thus provides, in a further aspect, an aqueous composition comprising ozone bubbles for use in skin activation. The explanation about the hair-growth effect also applies when the purpose is to activate the skin. The activation of the skin includes the activation of cells existing in and around the skin surface, such as the activation of epidermal cells and the activation of hair matrix cells. In particular, the ozone microbubble shower of the present invention is suitable for application to the scalp.
本発明のオゾンバブルを含む水性組成物とミネラル類とを併用して皮膚に適用した場合、さらなる皮膚活性化効果が得られる。皮膚活性化においても、ミネラル類および併用に関する上記説明があてはまる。 When the aqueous composition containing the ozone bubble of the present invention and minerals are used in combination with the skin, a further skin activating effect is obtained. The above explanation regarding minerals and combined use also applies to skin activation.
本発明は、別の態様において、上記水性組成物を動物の皮膚に適用することを特徴とする、皮膚の活性化方法を提供する。動物はヒトであってもよく、ヒト以外の動物であってもよい。例えば、イヌ、ネコなどのペット動物に本発明の方法を適用することができる。 In another aspect, the present invention provides a method for activating skin, characterized in that the aqueous composition is applied to animal skin. The animal may be a human or a non-human animal. For example, the method of the present invention can be applied to pet animals such as dogs and cats.
さらに本発明は、皮膚の活性化を要する動物の皮膚部位において皮膚を活性化させるための、上記水性組成物の使用を提供する。 Furthermore, the present invention provides the use of the above aqueous composition for activating skin at the skin site of an animal in need of skin activation.
以下に実施例を示して本発明をさらに詳細かつ具体的に説明するが、実施例は本発明の範囲を限定するものではない。 The present invention will be described in more detail and specifically with reference to the following examples. However, the examples do not limit the scope of the present invention.
実施例１ オゾンマイクロバブルシャワーによる皮膚刺激実験
３頭のイヌ（ビーグル、メス３頭）を用いてオゾンマイクロバブルシャワーによる表皮および毛根への影響について調査した。Example 1 Skin Irritation Experiment with Ozone Micro Bubble Shower Using three dogs (beagle, 3 females), the effect of ozone micro bubble shower on the epidermis and hair roots was investigated.
（ａ）実験方法
１）実験犬の体幹の背側に正中を境界としてそれぞれ左右に１カ所ずつ、５ｘ５ｃｍの正方形に毛刈りをした（０日とする）。
２）直径５ｍｍのデルマパンチで各剃毛部より１カ所ずつ皮膚を採取し、１０％ホルマリン液に浸漬した。
３）毛刈りをした部分より毛髪を数本採取し、毛髪の太さを測定した。
４）左側をマイクロバブルシャワー群（Ｍ群）、右側をオゾンマイクロバブルシャワー群（Ｏ群）とし、それぞれ５分間シャワーを適用した。シャワーを１日１回適用した。シャワーの圧力はイヌが不快に感じない範囲内で強くした。シャワーの温度は３３〜３６℃、水圧は１．５〜３．０ｋｇ／ｃｍ２とした。オゾンマイクロバブルは平均１．５〜２．０Ｌ／ｍｉｎであった。本処置を１ヶ月間実施した。
５）１４および３０日目に直径３５ｍｍのデルマパンチで各剃毛部より１カ所ずつ皮膚を採取した。またこのとき、毛刈りをした部分より毛髪を数本採取し、毛髪の太さを測定した。
６）１０％ホルマリン液に浸漬した組織片の標本を作製し、表皮および毛母細胞の観察を行った。表皮については無作為に５カ所を選び、その表皮層の厚さを測定した。毛母細胞については、毛根部を画像解析し、Ｒ，Ｇ，Ｂの色調を数値化して毛根部に占める割合を計測した。(A) Experimental method 1) The back of the trunk of the experimental dog was shaved into 5 × 5 cm squares on the left and right, respectively, with the midline as the boundary (referred to as day 0).
2) One skin was collected from each shaved portion with a derma punch having a diameter of 5 mm and immersed in a 10% formalin solution.
3) Several hairs were collected from the shaved portion and the thickness of the hair was measured.
4) The left side was a microbubble shower group (M group), the right side was an ozone microbubble shower group (O group), and a shower was applied for 5 minutes each. A shower was applied once a day. The shower pressure was increased within the range that dogs did not feel uncomfortable. The shower temperature was 33 to 36 ° C., and the water pressure was 1.5 to 3.0 kg / cm2 . Ozone microbubbles averaged 1.5 to 2.0 L / min. This treatment was performed for 1 month.
5) On the 14th and 30th days, one skin was collected from each shaved portion with a derma punch having a diameter of 35 mm. At this time, several hairs were collected from the shaved portion and the thickness of the hair was measured.
6) A specimen of a tissue piece immersed in 10% formalin solution was prepared, and the epidermis and hair matrix cells were observed. For the epidermis, five locations were selected at random, and the thickness of the epidermis layer was measured. For the hair matrix cells, the hair root part was image-analyzed, the color tone of R, G, B was digitized, and the proportion of the hair root part was measured.
（ｂ）結果
１）表皮の厚さ
１４日目に表皮断面の組織像を観察したところ、Ｍ群に比べてＯ群のほうが活性化した表皮細胞が多く観察された。図１に１４日目の表皮細胞の厚さを示した。３頭ともＭ群に比べてＯ群では有意に表皮層が厚くなっていた。(B) Results 1) Thickness of the epidermis When the tissue image of the cross section of the epidermis was observed on the 14th day, more epidermal cells were activated in the O group than in the M group. FIG. 1 shows the thickness of the epidermal cells on the 14th day. In all three animals, the epidermis layer was significantly thicker in the O group than in the M group.
２）毛髪の太さ
処置１４日目に毛髪の太さを測定した。処置前の毛髪の太さを１００とすると、Ｍ群では１２５、Ｏ群では１５０であり（いずれも３頭の平均）、毛髪が太くなっていることが確認された。2) Hair thickness The hair thickness was measured on the 14th day of treatment. When the thickness of the hair before the treatment was 100, it was 125 in the M group and 150 in the O group (both averaged for 3), and it was confirmed that the hair was thick.
３）毛根部の密度
図２に１４日後の毛根部の組織像を示した。Ｍ群に比べてＯ群のほうが毛根部の細胞密度が高いことが確認できた。また図３に処置３０日目の毛根部のＲＧＢ画像解析結果を示した。どの色調もＭ群に比べてＯ群において数値が有意に低かった。すなわち、どの色調もＭ群に比べてＯ群において密度が濃いことが示された。
４）使用感
Ｏ群において試験中に不快感を示したイヌは確認されなかった。3) Density of hair root part Fig. 2 shows a tissue image of the hair root part 14 days later. It was confirmed that the cell density of the hair root portion was higher in the O group than in the M group. FIG. 3 shows the RGB image analysis results of the hair root on the 30th day of treatment. Each color tone was significantly lower in the O group than in the M group. That is, it was shown that any color tone has a higher density in the O group than in the M group.
4) Feeling of use In the group O, dogs that showed discomfort during the test were not confirmed.
（ｃ）考察
本実験結果より、オゾンマイクロバブルシャワーを皮膚に適用することにより表皮細胞および毛母細胞が活性化することが明らかとなった。これらの結果は、本発明のオゾンマイクロバブルシャワーを皮膚に適用することによって育毛を促進できること、および皮膚を活性化できることを示すものである。(C) Discussion From the results of this experiment, it was revealed that the application of ozone microbubble shower to the skin activates epidermal cells and hair matrix cells. These results indicate that the hair growth can be promoted and the skin can be activated by applying the ozone microbubble shower of the present invention to the skin.
実施例２ オゾンマイクロバブルシャワーの表皮細胞に及ぼす影響
３頭のイヌ（ビーグル、メス３頭）を用いてオゾンマイクロバブルシャワーの表皮細胞に及ぼす影響を調べた。
（ａ）実験方法
１）イヌの背部皮膚を６箇所剪毛（１０ｘ１０ｃｍ）した。
２）試験前に剪毛部の組織を実施例１と同様の方法で採取した（処置前）。
３）左側をマイクロバブルシャワー群（Ｍ群）、右側をオゾンマイクロバブルシャワー群（Ｏ群）とし、それぞれ５分間シャワーを適用した。シャワーを１日１回適用した。シャワーの圧力はイヌが不快に感じない範囲内で強くした。シャワーの温度は３３〜３６℃、水圧は１．５〜３．０ｋｇ／ｃｍ２とした。オゾンマイクロバブルは平均１．５〜２．０Ｌ／ｍｉｎであった。
４）処置１４日目の組織を採取した。
５）Ｋｉ−６７染色により組織検査を行った。Ｋｉ−６７染色は常法により行った。Example 2 Effect of Ozone Micro Bubble Shower on Epidermal Cells The effect of ozone micro bubble shower on epidermal cells was examined using 3 dogs (beagle, 3 females).
(A) Experimental method 1) The dog's back skin was shaved (10 × 10 cm) at 6 points.
2) Before the test, the tissue of the shaved part was collected in the same manner as in Example 1 (before treatment).
3) The left side was a micro bubble shower group (M group), the right side was an ozone micro bubble shower group (O group), and a shower was applied for 5 minutes each. A shower was applied once a day. The shower pressure was increased within the range that dogs did not feel uncomfortable. The shower temperature was 33 to 36 ° C., and the water pressure was 1.5 to 3.0 kg / cm2 . Ozone microbubbles averaged 1.5 to 2.0 L / min.
4) Tissues on the 14th day of treatment were collected.
5) Histological examination was performed by Ki-67 staining. Ki-67 staining was performed by a conventional method.
（ｂ）結果
図４〜６に示すように、いずれのイヌにおいても、本発明のオゾンマイクロバブルシャワーを適用した部分の組織中にＫｉ−６７陽性細胞が多く認められた。これらの結果を図７にまとめた。すなわち、Ｋｉ−６７陽性細胞率は、マイクロバブルシャワー（オゾンのかわりに空気を使用）を適用した部分のＫｉ−６７陽性細胞率よりも高かった。(B) Result As shown in FIGS. 4-6, in any dog, many Ki-67 positive cells were recognized in the tissue of the part to which the ozone microbubble shower of the present invention was applied. These results are summarized in FIG. That is, the Ki-67 positive cell rate was higher than the Ki-67 positive cell rate of the part to which microbubble shower (using air instead of ozone) was applied.
（ｃ）考察
上記結果から、本発明のオゾンマイクロバブルシャワーが表皮細胞の増殖を活性化させることが明らかとなった。このことから、本発明のオゾンマイクロバブルシャワーが毛母細胞などの育毛にかかわる細胞の増殖を活性化するものと考えられた。(C) Discussion From the above results, it was revealed that the ozone microbubble shower of the present invention activates the proliferation of epidermal cells. From this, it was considered that the ozone microbubble shower of the present invention activated the proliferation of cells involved in hair growth such as hair matrix cells.
実施例３ 皮膚炎のイヌに対するオゾンマイクロバブルシャワーの効果
皮膚疾患（発赤、かゆみ、フケ等）を有する犬１１例に対して、オゾンマイクロバブルシャワーを１回使用した。シャワーの圧力はイヌが不快に感じない範囲内で強くした。シャワーの温度は３３〜３６℃、水圧は１．５〜３．０ｋｇ／ｃｍ２とした。オゾンマイクロバブルは平均１．５〜２．０Ｌ／ｍｉｎであった。本処置を１ヶ月間実施した。５例において皮膚症状の改善が見られた。典型的な症例について以下に説明する。
Example 3 Effect of Ozone Micro Bubble Shower on Dogs with Dermatitis Ozone micro bubble shower was used once for 11 dogs with skin diseases (redness, itching, dandruff, etc.). The shower pressure was increased within the range that dogs did not feel uncomfortable. The shower temperature was 33 to 36 ° C., and the water pressure was 1.5 to 3.0 kg / cm2 . Ozone microbubbles averaged 1.5 to 2.0 L / min. This treatment was performed for 1 month. Improvement of skin symptoms was seen in 5 cases. A typical case is described below.
症例は若齢時期よりアレルギー性皮膚炎に罹患し、薬用シャンプー等の内科療法を実施していたが、改善が見られなかった（図８左パネル）。オゾンマイクロバブルシャワーを週１回約５分間実施した。実施直後より臭気が減少し、１週間目には皮膚の発赤も軽減した。処置後１ヶ月目には、臭気、発赤も完全に消失し、脱毛部分には育毛もみられるほどに著しい改善がみられた（図８右パネル）。 The patient suffered from allergic dermatitis from a young age and had undergone medical treatment such as medicinal shampoo, but no improvement was observed (left panel in FIG. 8). An ozone microbubble shower was performed once a week for about 5 minutes. The odor decreased immediately after the implementation, and redness of the skin was reduced in the first week. One month after the treatment, the odor and redness disappeared completely, and a marked improvement was seen to the extent that hair growth was seen in the hair loss part (FIG. 8, right panel).
実施例４ ヒトにおけるオゾンマイクロバブルシャワーの効果
（ａ）方法
５０代のボランティア（男４名、女２名）に本発明のオゾンマイクロバブルシャワーを使用してもらい、頭髪や頭皮の状態について回答を得た。ボランティアは１日１〜２回約５分間オゾンマイクロバブルシャワーを頭皮に適用した。シャワーの圧力は人ボランテアが不快に感じない範囲内で強くした。シャワーの温度は３６〜３９℃、水圧は１．５〜３．０ｋｇ／ｃｍ２とした。オゾンマイクロバブルは平均１．５〜２．０Ｌ／ｍｉｎであった。本処置を３ヶ月間実施した。Example 4 Effect of Ozone Micro Bubble Shower in Human (a) Method 50 volunteers (4 men and 2 women) used the ozone micro bubble shower of the present invention and answered about the condition of the hair and scalp. Obtained. Volunteers applied an ozone microbubble shower to the scalp once or twice a day for about 5 minutes. The shower pressure was increased within the range that human volunteers did not feel uncomfortable. The shower temperature was 36 to 39 ° C., and the water pressure was 1.5 to 3.0 kg / cm2 . Ozone microbubbles averaged 1.5 to 2.0 L / min. This treatment was carried out for 3 months.
（ｂ）結果
１）抜け毛の状況
抜けなくなった １６％
抜け毛が減少 ６７％
変化なし １７％
悪化 ０％
２）髪の量
大幅に増加 ０％
少し増加 ５０％
変化なし ３３％
減少 １７％
３）ふけの量
大幅に減少 ０％
減少 １７％
変化なし ８３％
少し増加 ０％
４）髪のつや
出て来た ８３％
変化なし １６％
５）髪の状況（複数回答可能）
しっとりしてきた ８３％
さらさら感が出て来た ６７％
ぱさぱさ感がない １７％
白髪が黒くなった １７％
６）効果の発現時期
１）〜５）のいずれかにおいてプラスの効果が見られた時期について尋ねた。
１週間以内 １７％
２週間以内 １７％
１ヶ月以内 １７％
１ヶ月以上 ４９％
７）肌（頭皮）の状況
肌の状態が全体的に改善 ５０％
乾燥肌が改善 １７％
温泉に入ったよう １７％
変化なし １７％(B) Results 1) The situation of hair loss is no longer missing 16%
Hair loss decreased 67%
No change 17%
0% worse
2) Significant increase in hair volume 0%
Slight increase 50%
No change 33%
Decrease 17%
3) Significant decrease in dandruff amount 0%
Decrease 17%
No change 83%
Slight increase 0%
4) 83% came out of glossy hair
No change 16%
5) Hair situation (multiple answers possible)
83% have been moist
67% feels smooth
17% with no sensation
17% gray hair turned black
6) Time of onset of effect The time when a positive effect was observed in any one of 1) to 5) was asked.
Within 1 week 17%
Within 2 weeks 17%
Within 1 month 17%
More than 1 month 49%
7) The condition of the skin (scalp) Overall skin condition is improved 50%
Improved dry skin 17%
17% like a hot spring
No change 17%
今回のアンケート調査結果より、本発明のオゾンマウクロバブルシャワーを使用することにより、抜け毛の減少、髪のつやの発生を感じる被験者が約８割に達することが明らかとなった。髪の状態に関しても、しっとり感、さらさら感を感じる被験者が多かった。頭皮に関しては、８割以上の被験者が何らかのよい印象を感じたことが判明した。これらの効能は男女において偏りはなかった。 From the results of this questionnaire survey, it became clear that about 80% of the subjects felt the loss of hair loss and the occurrence of hair shine by using the ozone-macro bubble shower of the present invention. There were many subjects who felt moist and smooth about the hair condition. Regarding the scalp, it was found that more than 80% of the subjects felt some good impression. These effects were not biased between men and women.
本発明は、ヘルスケア産業、医薬、化粧品などの分野において利用可能である。 The present invention can be used in fields such as the healthcare industry, medicine, and cosmetics.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011229754AJP2013087092A (en) | 2011-10-19 | 2011-10-19 | Hair growth promotion effect of ozone microbubble shower |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011229754AJP2013087092A (en) | 2011-10-19 | 2011-10-19 | Hair growth promotion effect of ozone microbubble shower |
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| JP2013087092Atrue JP2013087092A (en) | 2013-05-13 |
| Application Number | Title | Priority Date | Filing Date |
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| JP2011229754APendingJP2013087092A (en) | 2011-10-19 | 2011-10-19 | Hair growth promotion effect of ozone microbubble shower |
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| JP (1) | JP2013087092A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017119661A (en)* | 2015-12-29 | 2017-07-06 | 株式会社ネオーラＭｂ研究所 | Cosmetics and production method thereof |
| JP2023175960A (en)* | 2020-11-13 | 2023-12-12 | レネロファーマ株式会社 | Skin symptom improvement agent and improvement method |
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|---|---|---|---|---|
| JPH08107936A (en)* | 1994-10-12 | 1996-04-30 | Mouhatsu Kurinitsukuriibu 21:Kk | Hair growth method and device |
| JP2003073236A (en)* | 2001-09-05 | 2003-03-12 | Kenkosha:Kk | Hair tonic, hair-restoring appliance and hair-restoring system using these |
| JP2009292783A (en)* | 2008-06-06 | 2009-12-17 | Dainichiseika Color & Chem Mfg Co Ltd | Wound treating composition, method for producing wound treating composition and skin external preparation |
| JP2009297692A (en)* | 2008-06-17 | 2009-12-24 | Sharp Corp | Water treatment apparatus and method |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08107936A (en)* | 1994-10-12 | 1996-04-30 | Mouhatsu Kurinitsukuriibu 21:Kk | Hair growth method and device |
| JP2003073236A (en)* | 2001-09-05 | 2003-03-12 | Kenkosha:Kk | Hair tonic, hair-restoring appliance and hair-restoring system using these |
| JP2009292783A (en)* | 2008-06-06 | 2009-12-17 | Dainichiseika Color & Chem Mfg Co Ltd | Wound treating composition, method for producing wound treating composition and skin external preparation |
| JP2009297692A (en)* | 2008-06-17 | 2009-12-24 | Sharp Corp | Water treatment apparatus and method |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017119661A (en)* | 2015-12-29 | 2017-07-06 | 株式会社ネオーラＭｂ研究所 | Cosmetics and production method thereof |
| JP2023175960A (en)* | 2020-11-13 | 2023-12-12 | レネロファーマ株式会社 | Skin symptom improvement agent and improvement method |
| JP7636758B2 (en) | 2020-11-13 | 2025-02-27 | レネロファーマ株式会社 | Agent for improving skin symptoms and method for improving skin symptoms |
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