【０００１】[0001]
【発明の属する技術分野】本発明は、ヒトの肌状態を改
善するための肌改善用食品組成物、及び肌改善方法に関
する。TECHNICAL FIELD The present invention relates to a skin improving food composition for improving human skin condition and a skin improving method.
【０００２】[0002]
【従来の技術】卵殻膜は、鶏卵等の卵殻の内面を覆って
いる皮膜状の膜であり、その成分は、水分以外におい
て、タンパク質約９０％、脂質約３％、糖類約２％であ
ると言われている。卵殻膜は、熱傷等による創傷部を被
覆すると、卵殻膜が創傷部に密着し、皮膚の上皮形成が
促進されることが知られているが、卵殻膜はカルシウム
源等として利用されている卵殻を採取する際に発生する
ものであり、従来は、その殆どを廃棄していた。しかし
ながら、近年の産業廃棄物の問題や、卵殻膜又はその加
水分解物が皮膚内のIII型コラーゲンの合成亢進作用を
有することが分り、卵殻膜を化粧品の原料として利用す
るようになった。そして経口用途に付いては、特公昭５
５−４５１７６号公報には飲食物の滋養増強方法、特開
平６−６５０９４号公報には免疫賦活・感染防御剤、特
開平９−４０５６４号公報には悪酔予防改善組成物等、
種々提案されている。2. Description of the Related Art The eggshell membrane is a film-like membrane that covers the inner surface of eggshells such as chicken eggs, and its components are, other than water, about 90% protein, about 3% lipid, and about 2% saccharide. Is said. The eggshell membrane is known to cover the wound due to burns and the like, and the eggshell membrane adheres to the wound to promote epithelial formation of the skin. The eggshell membrane is used as a calcium source, etc. It occurs when collecting, and conventionally, most of it was discarded. However, in recent years, it has been found that the eggshell membrane or its hydrolyzate has an action of promoting the synthesis of type III collagen in the skin, and the eggshell membrane has come to be used as a raw material for cosmetics. And for oral use, Japanese Patent Publication Sho 5
No. 5-45176 discloses a method for enhancing nutrition of food and drink, JP-A No. 6-65094 discloses an immune activating / infection-preventing agent, and JP-A No. 9-40564 discloses a composition for improving sickness prevention.
Various proposals have been made.
【０００３】しかしながら、化粧品等の外用剤では、卵
殻膜の使用量が少なく、上記経口用途に付いては、実際
に実用には至っていない。したがって、卵殻膜が十分に
利用されているとは言い難く、その多くが廃棄されてい
ることもあり、卵殻膜の更なる用途開発が切望されてい
る。However, in the external preparations such as cosmetics, the eggshell membrane is used in a small amount, and it has not been practically used for oral use. Therefore, it cannot be said that the eggshell membranes are fully utilized, and many of them are discarded, and further development of applications of the eggshell membranes is desired.
【０００４】一方、近年、健康に対する関心が高まり、
コンビニエンスストアーや通信販売等で簡単に栄養補助
食品を入手できるようになり、その市場が年々拡大して
いる。栄養補助食品には、一般的に、動植物の抽出物あ
るいはエキス、ビタミン類あるいはミネラルを有効成分
として配合されているが、卵殻膜又はその加水分解物は
未だ利用されておらず、経口摂取したときの肌改善効果
に付いても何ら知られていない。On the other hand, in recent years, interest in health has increased,
It has become easy to obtain nutritional supplements at convenience stores and mail-order stores, and the market is expanding year by year. Dietary supplements generally contain animal or plant extracts or extracts, vitamins or minerals as active ingredients, but eggshell membranes or their hydrolysates have not yet been used, and when ingested orally. There is nothing known about its skin improving effect.
【０００５】[0005]
【発明が解決しようとする課題】そこで本発明の目的
は、卵殻膜の新たな用途として、経口摂取により良好な
肌改善効果を示す卵殻膜又はその加水分解物、及びカキ
エキス、更にアスコルビン酸又はその塩を含有した肌改
善用食品組成物、及び肌改善方法を提供することであ
る。Therefore, an object of the present invention is, as a new use of the eggshell membrane, an eggshell membrane or a hydrolyzate thereof, which shows a good skin improving effect by oral ingestion, and an oyster extract, and ascorbic acid or the same. It is intended to provide a skin-improving food composition containing a salt and a skin-improving method.
【０００６】[0006]
【課題を解決するための手段】本発明者らは、上記の目
的を達成すべく卵殻膜の新たな機能について鋭意研究を
重ねた結果、意外にも卵殻膜とカキエキス、更にアスコ
ルビン酸と組合わせたものを経口摂取した場合に肌の状
態を改善する効果に優れていることを見出し本発明を完
成するに至った。すなわち、本発明は、（１）卵殻膜又
はその加水分解物、及びカキエキスを含有する肌改善用
食品組成物、（２）アスコルビン酸又はその塩を含有し
ている（１）の肌改善用食品組成物、（３）ヒトの肌を
改善する方法であって、（１）の肌改善用食品組成物
を、卵殻膜又はその加水分解物の総合計の摂取量が乾物
換算で１００ｍｇ／日以上、及びカキエキスの摂取量が
乾物換算で３０ｍｇ／日以上となるようにヒトに摂取さ
せる肌改善方法、（４）ヒトの肌を改善する方法であっ
て、（２）の肌改善用食品組成物を、卵殻膜又はその加
水分解物の総合計の摂取量が乾物換算で１００ｍｇ／日
以上、カキエキスの摂取量が乾物換算で３０ｍｇ／日以
上、及びアスコルビン酸又はその塩の総合計の摂取量が
乾物換算で８０ｍｇ／日以上となるようにヒトに摂取さ
せる肌改善方法、を提供することである。Means for Solving the Problems As a result of intensive studies on new functions of eggshell membranes to achieve the above object, the present inventors have surprisingly found that eggshell membranes, oyster extracts, and ascorbic acid are combined. The inventors have found that it is excellent in improving the skin condition when orally ingested, and thus completed the present invention. That is, the present invention provides (1) a skin-improving food composition containing an eggshell membrane or a hydrolyzate thereof and an oyster extract, (2) a skin-improving food containing (1) ascorbic acid or a salt thereof. A composition, (3) a method for improving human skin, wherein the total food intake of the eggshell membrane or its hydrolyzate is 100 mg / day or more in the food composition for skin improvement of (1). And a method for improving skin to be ingested by a human so that the oyster extract intake becomes 30 mg / day or more in terms of dry matter, (4) a method for improving human skin, wherein the food composition for improving skin is (2). The total intake of eggshell membrane or its hydrolyzate is 100 mg / day or more as dry matter, the intake of oyster extract is 30 mg / day or more as dry matter, and the total intake of ascorbic acid or its salt is 80mg / day or more in terms of dry matter It is to provide a method skin improvement to feed humans, to.
【０００７】[0007]
【発明の実施の形態】以下本発明を説明する。なお、本
発明において特に限定していない場合は、「％」は「質
量％」、また「部」は「質量部」を意味する。本発明の
肌改善用食品組成物は、卵殻膜又はその加水分解物、及
びカキエキス、更にアスコルビン酸又はその塩を含有す
る。BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described below. In the present invention, unless otherwise specified, "%" means "mass%" and "part" means "part by mass". The food composition for improving skin according to the present invention contains an eggshell membrane or a hydrolyzate thereof, an oyster extract, and ascorbic acid or a salt thereof.
【０００８】ここで、卵殻膜とは、鳥類の卵（特に鶏
卵）の殻部から常法により採取した湿潤状態のもの、該
湿潤状態のものを天日乾燥、乾燥機等で乾燥させたも
の、該乾燥物を粉砕機で粉砕したもの等が挙げられる。Here, the eggshell membrane means a wet state sampled from the shell portion of an avian egg (especially an egg) by a conventional method, and the wet state one dried by sun drying or a dryer. The dried product is crushed by a crusher.
【０００９】また、卵殻膜の加水分解物とは、卵殻膜
（湿潤物、乾燥物、粉状物を問わず）に加水分解処理を
施したものであり、加水分解処理としては、酸（例え
ば、塩酸、硫酸、硝酸、酢酸等）、アルカリ（例えば、
水酸化ナトリウム、水酸化カリウム等）、酵素（例え
ば、タンパク質分解酵素（パパイン、パンクレアチン
等））等による処理を挙げることができる。これらの処
理は単独でもよく、２種以上の処理を同時にもしくは別
々に組合わせて実施してもよい。The egg shell membrane hydrolyzate is obtained by subjecting an egg shell membrane (whether wet, dry, or powdered) to a hydrolysis treatment. , Hydrochloric acid, sulfuric acid, nitric acid, acetic acid, etc.), alkali (eg,
Examples thereof include treatment with sodium hydroxide, potassium hydroxide, etc.), enzymes (eg, proteolytic enzymes (papain, pancreatin, etc.)) and the like. These treatments may be carried out alone, or two or more treatments may be carried out simultaneously or in combination.
【００１０】このような加水分解処理の一般的な操作の
例を以下に具体的に説明する。An example of a general operation of such a hydrolysis treatment will be specifically described below.
【００１１】酸加水分解処理卵の殻部から得られた卵殻膜あるいは粉末状卵殻膜１部
（乾物換算）に対し、５〜４０部の０．５〜６ｍｏｌ／
ｌの塩酸あるいは０．２５〜８ｍｏｌ／ｌの硫酸を添加
し、例えば常圧下で５０〜１００℃で３０分〜２４時間
処理し、中和した後、濾過し、必要に応じて更に脱塩す
ることにより酸加水分解処理を施した卵殻膜水溶液を得
る。5 to 40 parts of 0.5 to 6 mol / per egg shell membrane or powdery eggshell membrane obtained from the shell portion of acid-hydrolyzed eggs
l hydrochloric acid or 0.25 to 8 mol / l sulfuric acid is added, for example, treated at 50 to 100 ° C. under normal pressure for 30 minutes to 24 hours, neutralized, filtered, and optionally desalted. As a result, an aqueous solution of eggshell membranes subjected to acid hydrolysis treatment is obtained.
【００１２】アルカリ加水分解処理卵の殻部から得られる卵殻膜あるいは粉末状卵殻膜１部
（乾物換算）に対し、５〜４０部の０．５〜３ｍｏｌ／
ｌの水酸化ナトリウムあるいは３０〜７０容量％のエタ
ノールを含有する０．５〜３ｍｏｌ／ｌの水酸化ナトリ
ウムを添加し、例えば４０〜１００℃で３０分〜８時間
処理し、中和した後、濾過し、必要に応じ更に脱塩する
ことによりアルカリ加水分解処理を施した卵殻膜水溶液
を得る。0.5 to 3 mol / mol of 5 to 40 parts per 1 part (dry matter equivalent) of the eggshell membrane or powdered eggshell membrane obtained from the shell portion of the alkali-hydrolyzed egg
1 sodium hydroxide or 0.5 to 3 mol / l sodium hydroxide containing 30 to 70% by volume of ethanol is added, and the mixture is treated at 40 to 100 ° C. for 30 minutes to 8 hours and neutralized, The solution is filtered and, if necessary, desalted to obtain an alkaline hydrolysis-treated eggshell membrane aqueous solution.
【００１３】酵素加水分解処理卵の殻部から得られる卵殻膜あるいは粉末状卵殻膜１部
（乾物換算）に対し、５〜４０部の水を加え、タンパク
質分解酵素（例えば、パパイン、パンクレアチン等）を
卵殻膜の乾物に対し０．５〜２０％となるように添加
し、酵素の至適ｐＨ及び温度範囲内で５〜４０時間処理
し、その後加熱により酵素を失活させ、濾過することに
より酵素加水分解処理を施した卵殻膜水溶液を得る。5 to 40 parts of water is added to 1 part of the eggshell membrane or powdery eggshell membrane obtained from the shell portion of the enzymatically hydrolyzed egg (dry matter equivalent), and a proteolytic enzyme (eg papain, pancreatin, etc.) is added. ) Is added so as to be 0.5 to 20% with respect to the dry matter of the eggshell membrane, treated for 5 to 40 hours within the optimum pH and temperature range of the enzyme, and then the enzyme is inactivated by heating and filtered. To obtain an egg shell membrane aqueous solution that has been subjected to enzymatic hydrolysis.
【００１４】酸加水分解処理と酵素加水分解処理との併
用卵の殻部から得られた卵殻膜あるいは粉末状卵殻膜１部
（乾物換算）に対し、５〜４０部の０．５〜６ｍｏｌ／
ｌの塩酸あるいは０．２５〜８ｍｏｌ／ｌの硫酸を添加
し、例えば常圧下で５０〜１００℃で３０分〜２４時間
処理し、次に、酵素の至適ｐＨに調整した後、タンパク
質分解酵素（例えば、パパイン、パンクレアチン等）を
卵殻膜の乾物に対して０．１〜２０％となるように添加
し、酵素の至適温度範囲内で５〜４０時間処理し、その
後加熱により酵素を失活させ、濾過することにより酸加
水分解及び酵素加水分解処理を施した卵殻膜水溶液を得
る。Combined use of acid hydrolysis treatment and enzymatic hydrolysis treatment 5 to 40 parts of 0.5 to 6 mol / per egg shell membrane or powdery egg shell membrane obtained from the shell of an egg (converted to dry matter)
l hydrochloric acid or 0.25 to 8 mol / l sulfuric acid is added, and the mixture is treated, for example, under normal pressure at 50 to 100 ° C. for 30 minutes to 24 hours, and then adjusted to the optimum pH of the enzyme, and then the proteolytic enzyme is added. (For example, papain, pancreatin, etc.) is added so as to be 0.1 to 20% with respect to the dry matter of the eggshell membrane, treated for 5 to 40 hours within the optimum temperature range of the enzyme, and then heated to remove the enzyme. The eggshell membrane aqueous solution that has been subjected to acid hydrolysis and enzymatic hydrolysis treatment is obtained by deactivating and filtering.
【００１５】なお、上述の各種の加水分解処理により得
られる卵殻膜は、水溶液の状態であり、その状態でも使
用することができるが、例えば、凍結乾燥法あるいは噴
霧乾燥法等により乾燥し、必要に応じて粉末化した乾物
状態にある卵殻膜が本発明の経口用食品組成物として使
用する際に、保存性に優れ、取り扱いやすいこと等の点
で好ましい。また、加水分解処理を施していない卵殻膜
に付いても同様に乾物状態が好ましい。ここで、乾物状
態にある卵殻膜とは、該卵殻膜２ｇをガラス秤量瓶に精
秤し、１０５℃で４時間乾燥して減少した質量分を水分
とした時、該卵殻膜の水分量が１０％以下の状態にある
ことを意味し、後述するカキエキス、アスコルビン酸又
はその塩の乾燥状態に付いても、同様な状態にあること
を意味する。The eggshell membranes obtained by the above-mentioned various hydrolysis treatments are in the state of an aqueous solution and can be used in that state. For example, they are required to be dried by a freeze-drying method or a spray-drying method. In accordance with the above, a powdered eggshell membrane in a dry matter state is preferable in terms of excellent storage stability and easy handling when used as the oral food composition of the present invention. In addition, the dry matter state is also preferable for the eggshell membrane not subjected to the hydrolysis treatment. Here, the eggshell membrane in a dry state means that when 2 g of the eggshell membrane is precisely weighed in a glass weighing bottle and dried at 105 ° C. for 4 hours and the reduced mass is taken as water, the water content of the eggshell membrane is It means that the oyster extract, ascorbic acid or a salt thereof, which will be described later, is in a similar state even when it is in a state of 10% or less.
【００１６】次に、本発明の肌改善用食品組成物に使用
するカキエキスに付いて説明する。本発明のカキエキス
とは、二枚貝綱イタボガキ科に属するカキのカキ肉を水
（特に、温水）抽出し、固形分を除去した後の上清を濃
縮したもの、あるいは乾燥したものである。また、さら
に前記上清あるいは濃縮液を限外濾過膜による方法等に
より分画したものも、本発明のカキエキスに含まれる。
このようなカキエキスには、一般的に、亜鉛等のミネラ
ル類、アミノ酸、タウリン、ビタミン類、ペプタイド、
アデノシンモノホスフェート等の核酸関連物質、ムコ多
糖、タンパク質等の成分が含有している。Next, the oyster extract used in the food composition for improving skin of the present invention will be described. The oyster extract of the present invention is obtained by extracting oyster meat of an oyster belonging to the family Bivalvia and other species, water (particularly, warm water), removing the solids, and concentrating the supernatant, or dried. Further, the oyster extract of the present invention also includes the supernatant or concentrated solution fractionated by a method using an ultrafiltration membrane.
Such oyster extracts generally include minerals such as zinc, amino acids, taurine, vitamins, peptides,
It contains nucleic acid-related substances such as adenosine monophosphate, components such as mucopolysaccharides and proteins.
【００１７】カキエキスの具体的な製造例を説明する
と、新鮮なカキ肉に対し同量あるいは数倍量の清水を添
加し、約４０〜９０℃、好ましくは６０〜８５℃で、１
〜４時間、好ましくは２〜３時間抽出を行う。そして、
濾紙や濾布等で固形分を除去し、得られた抽出液を濃縮
する、あるいは抽出液を噴霧乾燥、凍結乾燥、ドラム乾
燥等で乾燥し、必要に応じて粉砕してカキエキスを得
る。特に、乾物状態にあるカキエキスは、本発明の経口
用食品組成物として使用する際に、保存性に優れ、取り
扱いやすいこと等の点で好ましい。Explaining a concrete example of the production of oyster extract, the same amount or several times as much fresh water is added to fresh oyster meat, and the oyster extract is added at about 40 to 90 ° C, preferably 60 to 85 ° C.
Extraction is carried out for -4 hours, preferably 2-3 hours. And
The solid content is removed with filter paper, filter cloth or the like, and the obtained extract is concentrated, or the extract is dried by spray drying, freeze drying, drum drying, etc., and crushed as necessary to obtain an oyster extract. In particular, the oyster extract in the dry state is preferable in terms of excellent storage stability and easy handling when used as the oral food composition of the present invention.
【００１８】また、本発明で使用するアスコルビン酸又
はその塩は、市販のものを用いれば良く、アスコルビン
酸の塩としては、例えば、ナトリウム塩、カリウム塩、
カルシウム塩等が挙げられる。The ascorbic acid or salt thereof used in the present invention may be a commercially available one, and examples of the salt of ascorbic acid include sodium salt, potassium salt,
Calcium salt etc. are mentioned.
【００１９】本発明の肌改善用食品組成物は、上述した
卵殻膜又はその加水分解物、及びカキエキスを含有し
た、好ましくは、更にアスコルビン酸又はその塩を含有
した肌改善のために食する食品である。その形態は、一
般的に健康食品と称される食品の形態と同様、錠剤タイ
プ、カプセルタイプ、顆粒タイプ、液状タイプ等が挙げ
られる。The food composition for improving skin according to the present invention contains the above-mentioned eggshell membrane or a hydrolyzate thereof and an oyster extract, and preferably contains ascorbic acid or a salt thereof, which is eaten for improving skin. Is. The form thereof includes a tablet type, a capsule type, a granule type, a liquid type and the like similar to the form of a food generally called a health food.
【００２０】また、肌改善用食品組成物中の上記成分の
含有量は、後述する１日の摂取量により適宜設定すれば
よい。The content of the above components in the skin-improving food composition may be appropriately set according to the daily intake described below.
【００２１】本発明の食品組成物は、本発明の効果を損
なわない範囲で他の栄養成分や賦形剤等の各種食品原料
を適宜選択し含有させると良い。例えば、ビタミン
Ｂ２、ビタミンＢ１２、ビタミンＥ等のビタミン類、核
酸、ヒアルロン酸、コンドロイチン硫酸、コラーゲン等
の栄養成分、鉄、カルシウム、亜鉛等のミネラル成分、
エイコサペンタエン酸、ドコサヘキサエン酸、アラキド
ン酸等の高度不飽和脂肪酸等の各種栄養成分、増量剤、
結合剤、滑沢剤、保存剤、酸化防止剤、香料等の賦形剤
等が挙げられる。In the food composition of the present invention, various food ingredients such as other nutritional components and excipients may be appropriately selected and contained within a range that does not impair the effects of the present invention. For example, vitamins such as vitamin B2 , vitamin B12 , and vitamin E, nucleic acids, nutritional components such as hyaluronic acid, chondroitin sulfate, collagen, mineral components such as iron, calcium and zinc,
Eicosapentaenoic acid, docosahexaenoic acid, various nutritional ingredients such as highly unsaturated fatty acids such as arachidonic acid, bulking agents,
Examples include binders, lubricants, preservatives, antioxidants, and excipients such as fragrances.
【００２２】本発明の肌改善用食品組成物は、卵殻膜又
はその加水分解物、及びカキエキス、更にアスコルビン
酸又はその塩を一原料として用いる他は常法に従って製
造することができる。例えば、錠剤タイプを例として説
明すると、卵殻膜又はその加水分解物、及びカキエキ
ス、更にアスコルビン酸又はその塩、そしてその他の栄
養成分や賦形剤等を秤量し、必要に応じ篩等で異物を除
去した後、全ての原料を混合機で均一に混合し、得られ
た混合物を打錠機で打錠すれば良い。The skin-improving food composition of the present invention can be produced by a conventional method except that eggshell membrane or its hydrolyzate, oyster extract, and ascorbic acid or its salt are used as a raw material. For example, to explain the tablet type as an example, eggshell membrane or its hydrolyzate, and oyster extract, ascorbic acid or its salt, and other nutritional ingredients, excipients, etc. are weighed, and if necessary, remove foreign substances with a sieve or the like. After the removal, all the raw materials may be uniformly mixed with a mixer, and the obtained mixture may be tableted with a tableting machine.
【００２３】本発明の肌改善用食品組成物をヒトの肌改
善方法に適用した場合、平均的な成人（約６０ｋｇ）の
１日あたりの摂取量は、本発明の目的である肌改善効果
を得るために、卵殻膜又その加水分解物の摂取量が乾物
換算で好ましくは１００ｍｇ／日以上、より好ましくは
２００ｍｇ／日以上、カキエキスの摂取量が乾物換算で
好ましくは３０ｍｇ／日以上、より好ましくは５０ｍｇ
／日以上となる量である。更に優れた肌改善効果を得る
ためには、上記成分に加え、アスコルビン酸又はその塩
を本発明の肌改善用食品組成物に含有させることが好ま
しく、１日あたりの摂取量は、乾物換算で好ましくは８
０ｍｇ／日以上、より好ましくは１５０ｍｇ／日以上と
なる量である。なお、これらの成分の摂取量は、多すぎ
ても摂取量に見合う効果が得られないので、上記各成分
とも好ましくは２０００ｍｇ／日以下、より好ましくは
１５００ｍｇ／日以下とする。When the skin-improving food composition of the present invention is applied to the human skin-improving method, the average daily intake of an adult (about 60 kg) shows the skin-improving effect which is the object of the present invention. In order to obtain the egg shell membrane or its hydrolyzate, the intake amount thereof is preferably 100 mg / day or more, more preferably 200 mg / day or more, in terms of dry matter, and the oyster extract intake amount is preferably 30 mg / day or more, in terms of dry matter. Is 50 mg
/ It is the amount that is more than a day. In order to obtain a more excellent skin-improving effect, it is preferable to add ascorbic acid or a salt thereof to the skin-improving food composition of the present invention, in addition to the above components, and the daily intake is a dry matter equivalent. Preferably 8
The amount is 0 mg / day or more, more preferably 150 mg / day or more. The intake of these components does not produce an effect commensurate with the intake even if they are too large, and therefore the amount of each component is preferably 2000 mg / day or less, more preferably 1500 mg / day or less.
【００２４】本発明において、このような効果が得られ
る明確な理由は定かでないが、卵殻膜は、そのアミノ酸
組成において、シスチン、ヒスチジン及びプロリンが他
のタンパク質に比べて多く、また、カキエキスは、亜鉛
等のミネラル類、アミノ酸、タウリン、ビタミン類、ペ
プタイド、アデノシンモノホスフェート等の核酸関連物
質、ムコ多糖、タンパク質等の各種栄養成分を含有して
いることから、消化器官内におけるこれらのアミノ酸か
らなる分解生成物あるいはその他の成分の分解生成物
（卵殻膜及びカキエキス由来の分解物）が腸管等から吸
収された場合に、真皮内のヒアルロン酸やコラーゲン等
の皮膚の弾力性や柔軟性を担っている成分等の生成を促
進するからではないかと考えられる。また、アスコルビ
ン酸又はその塩を本発明の食品組成物に更に含有させる
と、アスコルビン酸は還元性を有する物質であることか
ら、腸管等から吸収された場合に、卵殻膜及びカキエキ
スによる真皮内のヒアルロン酸やコラーゲン等の生成促
進作用を更に助長するからではないかと考えられる。こ
こで、肌改善効果とは、肌の潤いを保持して肌荒れを改
善し、皮膚の弾力性や柔軟性を保持する効果である。In the present invention, although the clear reason why such an effect is obtained is not clear, the eggshell membrane has more cystine, histidine and proline in the amino acid composition than other proteins, and oyster extract has It contains minerals such as zinc, amino acids, taurine, vitamins, peptides, nucleic acid-related substances such as adenosine monophosphate, and various nutritional components such as mucopolysaccharides and proteins. When the decomposition products or the decomposition products of other components (decomposition products derived from eggshell membranes and oyster extracts) are absorbed from the intestinal tract, etc., they contribute to the elasticity and flexibility of the skin such as hyaluronic acid and collagen in the dermis. It is thought that this is because it promotes the production of existing components. Further, when ascorbic acid or a salt thereof is further contained in the food composition of the present invention, since ascorbic acid is a substance having a reducing property, when it is absorbed from the intestinal tract or the like, the eggshell membrane and the oyster extract cause dermal This is probably because it further promotes the action of promoting the production of hyaluronic acid, collagen and the like. Here, the skin improving effect is an effect of maintaining the moisture of the skin to improve the rough skin, and to maintain the elasticity and flexibility of the skin.
【００２５】次に、本発明を実施例及び試験例に基づ
き、さらに詳細に説明する。なお、本発明はこれらに限
定されるものではない。Next, the present invention will be described in more detail based on examples and test examples. The present invention is not limited to these.
【００２６】[0026]
【実施例】［実施例１］＜卵殻膜の製造方法＞まず、殻付き鶏卵を割卵して卵液
を除いた後、残りの卵殻膜付きの卵殻を粗粉砕した。得
られた粗粉砕物を清水中に入れて撹拌し、卵殻から分離
して浮上してきた卵殻膜を採取し、遠心式脱水機で脱水
し、湿潤状態の卵殻膜（水分量７８％）を得た。得られ
た湿潤卵殻膜を乾燥機（ヤマト科学（株）製、「ＤＶ−
４１」）で乾燥して乾燥卵殻膜を製した。そして、乾燥
卵殻膜を粉砕機（岡田精工（株）製、「ニュースピード
ミルＮＤ−１０型」）で粉砕し、水分含量が約４％の粉
末状の卵殻膜を得た。Examples [Example 1] <Method for producing eggshell membrane> First, eggs with shells were split into eggs to remove the egg liquid, and then the remaining eggshells with eggshell membranes were roughly crushed. The obtained coarsely pulverized product is put into fresh water and stirred, and the egg shell membrane that has separated from the egg shell and floated is collected and dehydrated with a centrifugal dehydrator to obtain a wet egg shell membrane (water content 78%). It was The obtained wet eggshell membrane was dried with a dryer (Yamato Scientific Co., Ltd., “DV-
41 ") to produce a dried eggshell membrane. Then, the dried eggshell membrane was pulverized with a pulverizer ("New Speed Mill ND-10 Model" manufactured by Okada Seiko Co., Ltd.) to obtain a powdered eggshell membrane having a water content of about 4%.
【００２７】＜カキエキスの製造方法＞新鮮なカキ肉１
０ｋｇに対し清水１０ｋｇを添加し、ゆっくり撹拌させ
ながた８５℃で２時間抽出を行ない、濾紙で固形分を除
去した。得られた抽出液を噴霧乾燥して水分含量約３％
の粉末状のカキエキスを得た。<Method for producing oyster extract> Fresh oyster meat 1
10 kg of clear water was added to 0 kg, extraction was performed at 85 ° C. for 2 hours while stirring slowly, and the solid content was removed with a filter paper. The obtained extract is spray-dried and the water content is about 3%.
A powdery oyster extract of
【００２８】＜肌改善用食品組成物の製造方法＞下記に
示す原料をそれぞれ秤量後、１６メッシュ篩で篩過す
る。次に、各原料を混合機に投入し、十分に混合して均
一とした後、１粒が約２８０ｍｇとなるように打錠機で
打錠し、錠剤タイプの肌改善用食品組成物を得た。この
肌改善用食品組成物を経口摂取した場合、優れた肌改善
効果を有していた。<Manufacturing method of food composition for improving skin> The following raw materials are weighed and then sieved with a 16 mesh sieve. Next, each raw material was put into a mixer and mixed sufficiently to make it uniform, and then tableted with a tableting machine so that one tablet was about 280 mg to obtain a tablet-type skin-improving food composition. It was When orally ingested, this skin-improving food composition had an excellent skin-improving effect.
【００２９】[0029]
【００３０】［実施例２］＜肌改善用食品組成物の製造＞実施例１の卵殻膜及びカ
キエキス、並びに下記に示す原料を用いて実施例１の肌
改善用食品組成物の製造方法と同様な方法で、錠剤タイ
プの肌改善用食品組成物を製した。得られた肌改善用食
品組成物を経口摂取した場合、優れた肌改善効果を有し
ていた。[Example 2] <Production of food composition for improving skin> Similar to the method for producing the food composition for improving skin of Example 1 using the eggshell membrane and oyster extract of Example 1 and the raw materials shown below. In this manner, a tablet type skin improving food composition was produced. When the obtained food composition for improving skin was orally ingested, it had an excellent skin improving effect.
【００３１】[0031]
【００３２】［実施例３］＜卵殻膜加水分解物の製造＞実施例１の卵殻膜の製造途
中で得られた湿潤卵殻膜２ｋｇに、１ｍｏｌ／ｌ塩酸水
溶液５リットルを加え、９０℃で１５時間、加熱撹拌し
た。次いで、４ｍｏｌ／ｌ水酸化ナトリウム水溶液でｐ
Ｈ５．５に調整し、タンパク質分解酵素（ノボノルディ
ックバイオインダストリー社製、「フレーバーザイムＭ
Ｇ」）４ｇを添加し、５５℃で４時間加温した後、ｐＨ
７．０に調整し、更に、フレーバーザイムＭＧを４ｇ添
加し、５０℃で４時間処理し、その後に９０℃で３０分
間処理して酵素を失活させた。次いで、活性炭２０ｇで
脱色し、得られた卵殻膜の加水分解物の溶液を噴霧乾燥
し、水分量が約３％の粉末状の卵殻膜加水分解物を得
た。Example 3 <Production of Eggshell Membrane Hydrolyzate> To 2 kg of the wet eggshell membrane obtained during the production of the eggshell membrane of Example 1 was added 5 liters of a 1 mol / l hydrochloric acid aqueous solution, and the mixture was heated at 90 ° C. for 15 minutes. The mixture was heated and stirred for hours. Then, p with 4 mol / l sodium hydroxide aqueous solution
Adjusted to H5.5, proteolytic enzyme (Nova Nordic Bio Industry, "Flavourzyme M
G ”) 4 g, and after heating at 55 ° C. for 4 hours,
The pH was adjusted to 7.0, 4 g of flavorzyme MG was further added, and the mixture was treated at 50 ° C. for 4 hours and then at 90 ° C. for 30 minutes to inactivate the enzyme. Next, the eggshell membrane hydrolyzate was decolorized with 20 g of activated carbon, and the resulting solution of the eggshell membrane hydrolyzate was spray-dried to obtain a powdery eggshell membrane hydrolyzate having a water content of about 3%.
【００３３】＜肌改善用食品組成物の製造方法＞実施例
２の卵殻膜を卵殻膜加水分解物に換えた以外は、実施例
２と同様な配合割合及び方法で、錠剤タイプの肌改善用
食品組成物を製した。得られた肌改善用食品組成物を経
口摂取した場合、優れた肌改善効果を有していた。<Method for producing food composition for improving skin> A tablet type skin improving agent was prepared in the same mixing ratio and method as in Example 2 except that the eggshell membrane of Example 2 was replaced with an eggshell membrane hydrolyzate. A food composition was made. When the obtained food composition for improving skin was orally ingested, it had an excellent skin improving effect.
【００３４】［比較例１］＜肌改善用食品組成物の比較例の製造方法＞下記に示す
原料を使用し、実施例１と同様な方法で製し、比較のた
めの肌改善用食品組成物を製した。[Comparative Example 1] <Method for producing comparative example of skin improving food composition> A skin improving food composition for comparison was prepared using the following raw materials in the same manner as in Example 1. I made a thing.
【００３５】[0035]
【００３６】［比較例２］＜肌改善用食品組成物の比較例の製造方法＞下記に示す
原料を使用し、実施例１と同様な方法で製し、比較のた
めの肌改善用食品組成物を製した。[Comparative Example 2] <Production method for comparative example of skin improving food composition> A skin improving food composition for comparison was prepared by using the following raw materials in the same manner as in Example 1. I made a thing.
【００３７】[0037]
【００３８】［試験例１］本発明の肌改善用食品組成物
が、肌改善効果に優れていることを立証するため、以下
の試験を行なった。[Test Example 1] In order to prove that the food composition for improving skin according to the present invention is excellent in the skin improving effect, the following tests were conducted.
【００３９】実施例１〜３の肌改善用食品組成物（本発
明品）、及び比較例１〜２の肌改善用食品組成物（比較
品）を、慢性的に肌が乾燥し、肌荒れに悩んでいる５０
名の女性（平均年齢３５歳）を被験者としてパネルテス
トを実施した。The skin-improving food compositions of Examples 1 to 3 (inventive products) and the skin-improving food compositions of Comparative Examples 1 to 2 (comparative products) were used to chronically dry and roughen the skin. 50 worried
A panel test was conducted by using a group of women (average age 35 years) as subjects.
【００４０】まず、肌の状態を確認するため、予め事前
アンケートを実施し、この事前アンケート結果を基に、
肌の状態が偏らないように１群１０名の５群に分けた。
１つの群に対し、上記何れか１つの錠剤タイプの肌改善
用食品組成物を朝夕２錠ずつ３週間にわたって摂取さ
せ、摂取前後の肌の状態についてアンケート調査を行っ
た。アンケート調査の内容は、肌の状態を以下の評価基
準に照らして回答させたものである。得られた結果を表
１に示す。なお、被験者にはどの錠剤を摂取させたか明
らかにしなかった。First, in order to confirm the condition of the skin, a preliminary questionnaire is conducted in advance, and based on the results of this preliminary questionnaire,
The skin condition was divided into 5 groups with 10 people per group.
One group was ingested with any one of the above tablet-type food compositions for improving skin for 2 weeks in the morning and evening for 3 weeks, and a questionnaire survey was conducted on the skin condition before and after ingestion. The content of the questionnaire survey is that the skin condition is answered according to the following evaluation criteria. The results obtained are shown in Table 1. In addition, it was not clarified which tablets the test subject had taken.
【００４１】＜肌状態評価基準＞ランク 肌状態Ａ： 非常に改善された場合Ｂ： 改善された場合Ｃ： やや改善された場合Ｄ： 変化が認められない場合<Skin Condition Evaluation Criteria>Rank skin conditionA: When it is greatly improvedB: When improvedC: When there is some improvementD: When no change is observed
【００４２】[0042]
【表１】[Table 1]
【００４３】表１の結果から、本発明の肌改善用食品組
成物は、肌改善効果に優れていることが理解される。特
に、アスコルビン酸を含有した実施例２及び３の本発明
品その効果に優れていた。なお、ここでは示していない
が、実施例２あるいは実施例３で使用しているアスコル
ビン酸に換えてアスコルビン酸ナトリウムを用いて同様
な試験を行なったところ、表１とほぼ同様な結果が得ら
れた。From the results shown in Table 1, it is understood that the food composition for improving skin of the present invention is excellent in the skin improving effect. Particularly, the products of Examples 2 and 3 containing ascorbic acid were excellent in their effects. Although not shown here, when a similar test was conducted using sodium ascorbate instead of ascorbic acid used in Example 2 or Example 3, almost the same results as in Table 1 were obtained. It was
【００４４】［試験例２］本発明の肌改善用食品組成物
の肌改善効果に対する卵殻膜又はその加水分解物、カキ
エキス、アスコルビン酸又はその塩の摂取量（乾物換
算）の影響を調べるために、以下の試験を行なった。[Test Example 2] In order to examine the effect of the intake (dry matter equivalent) of the eggshell membrane or its hydrolyzate, oyster extract, ascorbic acid or its salt on the skin improving effect of the food composition for improving skin of the present invention. The following tests were conducted.
【００４５】試験例１と同様にパネルテストを実施し
た。つまり、慢性的に肌が乾燥し、肌荒れに悩んでいる
４０名の女性（平均年齢３０歳）を被験者として、肌の
状態が偏らないように１群１０名の４群に分けた。各群
に実施例２の肌改善用食品組成物を３週間にわたり摂取
させ、摂取前後の肌の状態について試験例１の肌改善評
価基準に基づきアンケート調査を行った。なお、各群の
肌改善用食品組成物の摂取量は表２に示すとおりであ
り、各群における摂取の仕方は、１錠摂取の場合、朝１
錠、２錠摂取の場合、朝夕１錠ずつ、３錠摂取の場合、
朝２錠及び夕方１錠、４錠摂取の場合、朝夕２錠ずつと
した。A panel test was carried out in the same manner as in Test Example 1. That is, 40 women (average age 30 years old) suffering from chronic dry skin and rough skin were used as test subjects and divided into 4 groups of 10 people so that the skin condition was not biased. Each group was allowed to ingest the skin-improving food composition of Example 2 for 3 weeks, and a questionnaire survey was conducted on the skin condition before and after ingestion based on the skin-improvement evaluation criteria of Test Example 1. The intake amount of the skin-improving food composition of each group is as shown in Table 2, and the method of intake in each group is as follows:
If you take 2 tablets, 1 tablet each in the morning and evening, if you take 3 tablets,
In the case of 2 tablets in the morning and 1 tablet in the evening, 4 tablets were taken in the morning and 2 tablets each in the evening.
【００４６】[0046]
【表２】[Table 2]
【００４７】表２の結果から、ヒトの肌状態を改善する
には、本発明で使用する成分の摂取量が乾物換算で、好
ましくは卵殻膜が１００ｍｇ／日以上、カキエキスが３
０ｍｇ／日以上、アスコルビン酸が８０ｍｇ／日以上、
より好ましくは卵殻膜が２００ｍｇ／日以上、カキエキ
スが５０ｍｇ／日以上、アスコルビン酸が１５０ｍｇ／
日以上であることが理解される。なお、ここでは示して
いないが、実施例３の卵殻膜加水分解物を含有した肌改
善用食品組成物に付いて同様な試験を行なったところ、
表２とほぼ同様な結果が得られた。From the results shown in Table 2, in order to improve the human skin condition, the intake amount of the components used in the present invention is, as dry matter, preferably 100 mg / day or more of eggshell membrane and 3 of oyster extract.
0 mg / day or more, ascorbic acid 80 mg / day or more,
More preferably, the eggshell membrane is 200 mg / day or more, the oyster extract is 50 mg / day or more, and the ascorbic acid is 150 mg / day.
It is understood that it is more than a day. Although not shown here, a similar test was conducted on the skin improving food composition containing the eggshell membrane hydrolyzate of Example 3, and a similar test was conducted.
Results similar to those in Table 2 were obtained.
【００４８】[0048]
【発明の効果】以上述べたように、本発明の肌改善用食
品組成物は、経口摂取により良好な肌改善効果を示す。
そして、本発明により、卵殻膜の新たな用途を提供でき
ることから、卵殻膜の更なる需要拡大が期待されるとと
もに、産業廃棄物としての廃棄量を軽減できる。EFFECTS OF THE INVENTION As described above, the food composition for improving skin according to the present invention exhibits a good skin improving effect by oral ingestion.
Since the present invention can provide a new use of the eggshell membrane, further expansion of demand for the eggshell membrane can be expected, and the amount of waste as industrial waste can be reduced.
─────────────────────────────────────────────────────フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） Ａ６１Ｐ 17/00 Ａ６１Ｐ 17/00 Ｆターム(参考） 4B018 LE01 MD25 MD72 MD75 ME14 MF01 MF12 4C086 AA02 BA03 MA03 MA04 MA52 ZA89 ZC28 4C087 AA02 BB16 BB61 MA02 MA52 ZA89 ZC28─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl.7 Identification code FI theme code (reference) A61P 17/00 A61P 17/00 F term (reference) 4B018 LE01 MD25 MD72 MD75 ME14 MF01 MF12 4C086 AA02 BA03 MA03 MA04 MA52 ZA89 ZC28 4C087 AA02 BB16 BB61 MA02 MA52 ZA89 ZC28
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002047649AJP2003245055A (en) | 2002-02-25 | 2002-02-25 | Food composition for skin improvement and skin improvement method |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002047649AJP2003245055A (en) | 2002-02-25 | 2002-02-25 | Food composition for skin improvement and skin improvement method |
| Publication Number | Publication Date |
|---|---|
| JP2003245055Atrue JP2003245055A (en) | 2003-09-02 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002047649APendingJP2003245055A (en) | 2002-02-25 | 2002-02-25 | Food composition for skin improvement and skin improvement method |
| Country | Link |
|---|---|
| JP (1) | JP2003245055A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005145889A (en)* | 2003-11-14 | 2005-06-09 | Lion Corp | Pharmaceutical composition, bactericidal agent and oral composition containing eggshell membrane and amino compound |
| JP2008007419A (en)* | 2006-06-27 | 2008-01-17 | Ifuji Sangyo Co Ltd | Egg shell membrane hydrolyzate by proteolytic enzyme using chicken egg shell membrane as a raw material, its production method, and functional products to which these are added |
| JP2008061514A (en)* | 2006-09-05 | 2008-03-21 | Kanebo Cosmetics Inc | Microorganisms capable of resolving eggshell membrane proteins |
| JP2009120617A (en)* | 2009-03-03 | 2009-06-04 | Almado Co Ltd | Sports disorder inhibitor and functional food |
| US20140294961A1 (en)* | 2013-03-27 | 2014-10-02 | ALMADO Inc. | Hepatic protection agent containing eggshell membrane and pharmaceutical composition, food additive and food using the same |
| US20140356450A1 (en)* | 2013-05-28 | 2014-12-04 | ALMADO Inc. | Agent for activating sirtuin gene containing egg shell membrane ingredient and composition using the same |
| US20140363519A1 (en)* | 2013-06-11 | 2014-12-11 | The University Of Tokyo | Activator of gene expression of molecular chaperone gene comprising eggshell membrane component and composition thereof |
| US20150150916A1 (en)* | 2013-11-29 | 2015-06-04 | The University Of Tokyo | Insulin resistance-improving agent containing eggshell membrane component, and composition using the same |
| CN111434341A (en)* | 2019-01-15 | 2020-07-21 | 株式会社瑷兰梦朵 | Method for producing egg shell membrane in batches and method for producing egg-related product group |
| CN114304624A (en)* | 2020-10-12 | 2022-04-12 | 株式会社瑷兰梦朵 | Beverage containing eggshell membrane powder and hydrolyzed eggshell membrane component |
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| JPH11193279A (en)* | 1997-10-30 | 1999-07-21 | Q P Corp | Stabilizer and method for stabilizing ascorbic acids and preparations containing the same |
| WO1999064066A1 (en)* | 1998-06-09 | 1999-12-16 | Shionogi & Co., Ltd. | Immune tolerance-inducing agents containing hard keratin or keratin-like substance |
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPS6185322A (en)* | 1984-10-04 | 1986-04-30 | Osaka Chem Lab | Food for beautiful face and beautiful hair for preventing stain, or such |
| JPH11193279A (en)* | 1997-10-30 | 1999-07-21 | Q P Corp | Stabilizer and method for stabilizing ascorbic acids and preparations containing the same |
| WO1999064066A1 (en)* | 1998-06-09 | 1999-12-16 | Shionogi & Co., Ltd. | Immune tolerance-inducing agents containing hard keratin or keratin-like substance |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005145889A (en)* | 2003-11-14 | 2005-06-09 | Lion Corp | Pharmaceutical composition, bactericidal agent and oral composition containing eggshell membrane and amino compound |
| JP2008007419A (en)* | 2006-06-27 | 2008-01-17 | Ifuji Sangyo Co Ltd | Egg shell membrane hydrolyzate by proteolytic enzyme using chicken egg shell membrane as a raw material, its production method, and functional products to which these are added |
| JP2008061514A (en)* | 2006-09-05 | 2008-03-21 | Kanebo Cosmetics Inc | Microorganisms capable of resolving eggshell membrane proteins |
| JP2009120617A (en)* | 2009-03-03 | 2009-06-04 | Almado Co Ltd | Sports disorder inhibitor and functional food |
| US20140294961A1 (en)* | 2013-03-27 | 2014-10-02 | ALMADO Inc. | Hepatic protection agent containing eggshell membrane and pharmaceutical composition, food additive and food using the same |
| US20140356450A1 (en)* | 2013-05-28 | 2014-12-04 | ALMADO Inc. | Agent for activating sirtuin gene containing egg shell membrane ingredient and composition using the same |
| US20140363519A1 (en)* | 2013-06-11 | 2014-12-11 | The University Of Tokyo | Activator of gene expression of molecular chaperone gene comprising eggshell membrane component and composition thereof |
| US20150150916A1 (en)* | 2013-11-29 | 2015-06-04 | The University Of Tokyo | Insulin resistance-improving agent containing eggshell membrane component, and composition using the same |
| CN111434341A (en)* | 2019-01-15 | 2020-07-21 | 株式会社瑷兰梦朵 | Method for producing egg shell membrane in batches and method for producing egg-related product group |
| CN114304624A (en)* | 2020-10-12 | 2022-04-12 | 株式会社瑷兰梦朵 | Beverage containing eggshell membrane powder and hydrolyzed eggshell membrane component |
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