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FR2373795A1 - IMMUNOLOGICAL DOSAGE BY FIXED ENZYME - Google Patents

IMMUNOLOGICAL DOSAGE BY FIXED ENZYME

Info

Publication number
FR2373795A1
FR2373795A1FR7737194AFR7737194AFR2373795A1FR 2373795 A1FR2373795 A1FR 2373795A1FR 7737194 AFR7737194 AFR 7737194AFR 7737194 AFR7737194 AFR 7737194AFR 2373795 A1FR2373795 A1FR 2373795A1
Authority
FR
France
Prior art keywords
antigen
antibody
fixed enzyme
phase
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7737194A
Other languages
French (fr)
Other versions
FR2373795B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Italia SRL
Original Assignee
Carlo Erba SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carlo Erba SpAfiledCriticalCarlo Erba SpA
Publication of FR2373795A1publicationCriticalpatent/FR2373795A1/en
Application grantedgrantedCritical
Publication of FR2373795B1publicationCriticalpatent/FR2373795B1/fr
Grantedlegal-statusCriticalCurrent

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Abstract

Translated fromFrench

L'invention concerne la biochimie. Elle a pour objet un procédé de mise en évidence ou de dosage d'un antigène par contact dans un milieu liquide simultanément ou par stades, 1 d'un antigène porté par un support insoluble, 2 d'un échantillon contenant l'antigène à rechercher 3 d'un premier anticorps, et 4 d'un second anticorps marqué au moyen d'un enzyme, le second anticorps se fixant ainsi sur la phase insoluble en raison inverse de la quantité d'antigène à rechercher, l'activité enzymatique pouvant être ensuite determinée sur la phase solide ou la phase liquide. Le procédé est préféré pour les analyses de dépistage.The invention relates to biochemistry. It relates to a method for demonstrating or assaying an antigen by contact in a liquid medium simultaneously or in stages, 1 of an antigen carried by an insoluble support, 2 of a sample containing the antigen to be sought 3 of a first antibody, and 4 of a second antibody labeled with an enzyme, the second antibody thus binding to the insoluble phase in inverse proportion to the amount of antigen to be tested, the enzymatic activity being able to be then determined on the solid phase or the liquid phase. The method is preferred for screening assays.

FR7737194A1976-12-101977-12-09 IMMUNOLOGICAL DOSAGE BY FIXED ENZYMEGrantedFR2373795A1 (en)

Applications Claiming Priority (1)

Application NumberPriority DateFiling DateTitle
GB51709/76AGB1549069A (en)1976-12-101976-12-10Enzyme linked immunoassay

Publications (2)

Publication NumberPublication Date
FR2373795A1true FR2373795A1 (en)1978-07-07
FR2373795B1 FR2373795B1 (en)1982-09-10

Family

ID=10461077

Family Applications (1)

Application NumberTitlePriority DateFiling Date
FR7737194AGrantedFR2373795A1 (en)1976-12-101977-12-09 IMMUNOLOGICAL DOSAGE BY FIXED ENZYME

Country Status (26)

CountryLink
JP (1)JPS53101522A (en)
AR (1)AR226805A1 (en)
AU (1)AU517266B2 (en)
BE (1)BE861711A (en)
BR (1)BR7708181A (en)
CA (1)CA1110165A (en)
CH (1)CH628739A5 (en)
CS (1)CS208739B2 (en)
DE (1)DE2755008A1 (en)
DK (1)DK550077A (en)
ES (2)ES464929A1 (en)
FI (1)FI773718A7 (en)
FR (1)FR2373795A1 (en)
GB (1)GB1549069A (en)
GR (1)GR63580B (en)
HK (1)HK13881A (en)
HU (1)HU179956B (en)
IL (1)IL53576A (en)
IT (1)IT1109485B (en)
MY (1)MY8100367A (en)
NL (1)NL7713692A (en)
NO (1)NO774240L (en)
NZ (1)NZ185927A (en)
PT (1)PT67384B (en)
SE (1)SE7714034L (en)
TR (1)TR20650A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
EP0233044A3 (en)*1986-02-031990-07-25Cambridge Bioscience CorporationProcess for purifying recombinant proteins, and use of products thereof

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
JPS576363A (en)*1980-06-131982-01-13Takeda Chem Ind LtdProduction of specific antibody
CA1160566A (en)*1980-04-251984-01-17Harald GallatiImmunological determination method
ES8307897A1 (en)*1981-04-131983-08-01Hoechst Co AmericanSingle incubation immunochemical assay for creatin phosphokinase MB.
EP0084531B1 (en)*1981-07-311987-05-20Mount Sinai School Of Medicine Of The City University Of New YorkAssay for viruses
EP0072902B1 (en)*1981-08-211985-04-24F. HOFFMANN-LA ROCHE & CO. AktiengesellschaftMethod for the determination of carcinoembryonic antigen (cea) and suitable antibody solution for the determination
JPS58151559A (en)*1982-03-051983-09-08Takeda Chem Ind LtdImmunochemical measuring method of human villous gonadotropin and reagent therefor
US4704356A (en)*1984-03-271987-11-03Rush-Presbyterian-St. Luke's Medical CenterMethod of diagnosing cartilage tissue abnormalities
CH670709A5 (en)*1986-04-241989-06-30Univ Moskovsk
AT388618B (en)*1987-05-051989-08-10Binder Bernd Dr METHOD FOR QUANTITATIVE DETERMINATION OF FUNCTION AND ANTIGENT CONCENTRATION OF A SUBSTANCE CONTAINED IN A BIOLOGICAL LIQUID
DE3807440A1 (en)*1988-03-071989-09-21Progen Biotechnik GmbhMethod for the immunological detection of substances, and a composition and a test kit
DE19508264C1 (en)*1995-03-081996-02-01Klose Werner Dipl Ing FhMeasuring contours, esp. in road surface
US6808902B1 (en)1999-11-122004-10-26Amgen Inc.Process for correction of a disulfide misfold in IL-1Ra Fc fusion molecules

Citations (5)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
FR2126760A5 (en)*1971-02-101972-10-06Organon Nv
US3839153A (en)*1970-12-281974-10-01Akzona IncProcess for the detection and determination of specific binding proteins and their corresponding bindable substances
US3951748A (en)*1974-11-111976-04-20Medical Products, Inc.Sensitized matrix for detection of disease
FR2314499A1 (en)*1975-06-101977-01-07Weeke Bengt METHOD FOR DETERMINING ANTIBODIES IN BODY FLUIDS USING KNOWN ANTIGENS OR FOR DETERMINING ANTIGENS USING KNOWN ANTIBODIES FROM BODY FLUIDS
US4002532A (en)*1974-10-211977-01-11Weltman Joel KEnzyme conjugates

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
US3839153A (en)*1970-12-281974-10-01Akzona IncProcess for the detection and determination of specific binding proteins and their corresponding bindable substances
FR2126760A5 (en)*1971-02-101972-10-06Organon Nv
US4002532A (en)*1974-10-211977-01-11Weltman Joel KEnzyme conjugates
US3951748A (en)*1974-11-111976-04-20Medical Products, Inc.Sensitized matrix for detection of disease
FR2314499A1 (en)*1975-06-101977-01-07Weeke Bengt METHOD FOR DETERMINING ANTIBODIES IN BODY FLUIDS USING KNOWN ANTIGENS OR FOR DETERMINING ANTIGENS USING KNOWN ANTIBODIES FROM BODY FLUIDS

Cited By (2)

* Cited by examiner, † Cited by third party
Publication numberPriority datePublication dateAssigneeTitle
EP0233044A3 (en)*1986-02-031990-07-25Cambridge Bioscience CorporationProcess for purifying recombinant proteins, and use of products thereof
EP0596459A3 (en)*1986-02-031994-06-01Cambridge Biotech CorporationProcess for purifying recombinant proteins, and use of products thereof

Also Published As

Publication numberPublication date
AU517266B2 (en)1981-07-16
CS208739B2 (en)1981-09-15
ES468934A1 (en)1978-12-16
IL53576A0 (en)1978-03-10
CA1110165A (en)1981-10-06
DE2755008A1 (en)1978-06-15
IL53576A (en)1981-07-31
BR7708181A (en)1978-07-11
GR63580B (en)1979-11-22
HU179956B (en)1983-01-28
NL7713692A (en)1978-06-13
AR226805A1 (en)1982-08-31
SE7714034L (en)1978-06-11
IT1109485B (en)1985-12-16
AU3139577A (en)1979-06-14
GB1549069A (en)1979-08-01
MY8100367A (en)1981-12-31
HK13881A (en)1981-04-16
JPS53101522A (en)1978-09-05
DK550077A (en)1978-06-11
FR2373795B1 (en)1982-09-10
BE861711A (en)1978-03-31
NO774240L (en)1978-06-13
PT67384B (en)1979-05-18
NZ185927A (en)1980-02-21
TR20650A (en)1982-03-24
CH628739A5 (en)1982-03-15
FI773718A7 (en)1978-06-11
ES464929A1 (en)1979-01-01
PT67384A (en)1978-01-01

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