EP0472648A4 - Crosslinking oligonucleotides - Google Patents

Crosslinking oligonucleotides

Info

Publication number: EP0472648A4
Authority: EP; European Patent Office
Prior art keywords: crosslinking oligonucleotides; oligonucleotides; crosslinking
Prior art date: 1989-05-18
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Inventor

Rich B. Meyer

John C. Tabone

Gerald D. Hurst

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Nanogen Inc

Original Assignee

MicroProbe Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-05-18

Filing date

1990-05-15

Publication date

1992-09-16

1990-05-15Application filed by MicroProbe CorpfiledCriticalMicroProbe Corp

1992-03-04Publication of EP0472648A1publicationCriticalpatent/EP0472648A1/en

1992-09-16Publication of EP0472648A4publicationCriticalpatent/EP0472648A4/en

StatusWithdrawnlegal-statusCriticalCurrent

Links

108091034117OligonucleotideProteins0.000title1
JLCPHMBAVCMARE-UHFFFAOYSA-N[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphatePolymersCc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=OJLCPHMBAVCMARE-UHFFFAOYSA-N0.000title1
238000004132cross linkingMethods0.000title1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Immunology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Virology (AREA)
Saccharide Compounds (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

EP199009088441989-05-181990-05-15Crosslinking oligonucleotidesWithdrawnEP0472648A4 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US35385789A	1989-05-18	1989-05-18
US353857		1989-05-18

Publications (2)

Publication Number	Publication Date
EP0472648A1 EP0472648A1 (en)	1992-03-04
EP0472648A4true EP0472648A4 (en)	1992-09-16

Family

ID=23390880

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP19900908844WithdrawnEP0472648A4 (en)	1989-05-18	1990-05-15	Crosslinking oligonucleotides

Country Status (3)

Country	Link
EP (1)	EP0472648A4 (en)
JP (1)	JPH04507402A (en)
WO (1)	WO1990014353A1 (en)

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EP0615758B1 (en)*	1988-05-24	1999-09-22	Yeda Research And Development Co. Ltd.	Anti-tumor vaccines
US5824796A (en)*	1988-09-28	1998-10-20	Epoch Pharmaceuticals, Inc.	Cross-linking oligonucleotides
USRE38416E1 (en)	1988-09-28	2004-02-03	Epoch Biosciences, Inc.	Cross-linking oligonucleotides
US5849482A (en)*	1988-09-28	1998-12-15	Epoch Pharmaceuticals, Inc.	Crosslinking oligonucleotides
US5177196A (en)*	1990-08-16	1993-01-05	Microprobe Corporation	Oligo (α-arabinofuranosyl nucleotides) and α-arabinofuranosyl precursors thereof
EP0661979A4 (en)*	1991-08-21	1995-09-13	Microprobe Corp	Cross-linking oligonucleotides for enzyme-mediated triple strand formation.
US6136601A (en)*	1991-08-21	2000-10-24	Epoch Pharmaceuticals, Inc.	Targeted mutagenesis in living cells using modified oligonucleotides
AU6296294A (en)*	1993-01-26	1994-08-15	Microprobe Corporation	Bifunctional crosslinking oligonucleotides adapted for linking to a desired gene sequence of invading organism or cell
US6312894B1 (en)	1995-04-03	2001-11-06	Epoch Pharmaceuticals, Inc.	Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5801155A (en)	1995-04-03	1998-09-01	Epoch Pharmaceuticals, Inc.	Covalently linked oligonucleotide minor grove binder conjugates
ATE293123T1 (en)*	1997-09-12	2005-04-15	Exiqon As	BI- AND TRI-CYCLIC - NUCLEOSIDE, NUCLEOTIDE AND OLIGONUCLEOTIDE ANALOGS
US6054568A (en)*	1998-01-16	2000-04-25	The Perkin-Elmer Corporation	Nucleobase oligomers
US6127121A (en)	1998-04-03	2000-10-03	Epoch Pharmaceuticals, Inc.	Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination
EP1006199A1 (en)*	1998-12-03	2000-06-07	Kreatech Biotechnology B.V.	Applications with and methods for producing selected interstrand crosslinks in nucleic acid
US6660845B1 (en)	1999-11-23	2003-12-09	Epoch Biosciences, Inc.	Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US7205105B2 (en)	1999-12-08	2007-04-17	Epoch Biosciences, Inc.	Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
US20040081959A9 (en)	1999-12-08	2004-04-29	Epoch Biosciences, Inc.	Fluorescent quenching detection reagents and methods
US6727356B1 (en)	1999-12-08	2004-04-27	Epoch Pharmaceuticals, Inc.	Fluorescent quenching detection reagents and methods
US7348146B2 (en)	2003-10-02	2008-03-25	Epoch Biosciences, Inc.	Single nucleotide polymorphism analysis of highly polymorphic target sequences
CA2542768A1 (en)	2003-10-28	2005-05-12	Epoch Biosciences, Inc.	Fluorescent probes for dna detection by hybridization with improved sensitivity and low background
WO2005049849A2 (en)	2003-11-14	2005-06-02	Integrated Dna Technologies, Inc.	Fluorescence quenching azo dyes, their methods of preparation and use
CA2601554A1 (en)	2005-05-20	2006-11-30	Integrated Dna Technologies, Inc.	Compounds and methods for labeling oligonucleotides
DK2056845T3 (en)	2006-08-08	2017-11-27	Rheinische Friedrich-Wilhelms-Universität Bonn	STRUCTURE AND USE OF 5'-PHOSPHATE OLIGONUCLEOTIDES
LT2757091T (en)	2008-04-01	2017-09-11	Biosearch Technologies, Inc.	Stabilized Nucleic Acid Dark Quencher-Fluorophore Probes
EP2297323A1 (en)	2008-05-21	2011-03-23	Hartmann, Gunther	5' triphosphate oligonucleotide with blunt end and uses thereof
JP2012511927A (en)	2008-12-17	2012-05-31	ライフテクノロジーズコーポレーション	Methods, compositions, and kits for detecting allelic variants
US20100285478A1 (en)	2009-03-27	2010-11-11	Life Technologies Corporation	Methods, Compositions, and Kits for Detecting Allelic Variants
US9506057B2 (en)	2010-03-26	2016-11-29	Integrated Dna Technologies, Inc.	Modifications for antisense compounds
AU2011230496B2 (en)	2010-03-26	2015-09-17	Integrated Dna Technologies, Inc.	Methods for enhancing nucleic acid hybridization
WO2012033848A1 (en)	2010-09-07	2012-03-15	Integrated Dna Technologies, Inc.	Modifications for antisense compounds
EP2508530A1 (en)	2011-03-28	2012-10-10	Rheinische Friedrich-Wilhelms-Universität Bonn	Purification of triphosphorylated oligonucleotides using capture tags
CZ2012282A3 (en)	2012-04-25	2013-11-06	Contipro Biotech S.R.O.	Crosslinked hyaluronate derivative, process of its preparation, hydrogel and microfibers based thereon
EP2712870A1 (en)	2012-09-27	2014-04-02	Rheinische Friedrich-Wilhelms-Universität Bonn	Novel RIG-I ligands and methods for producing them
WO2014164874A2 (en)	2013-03-13	2014-10-09	Becton, Dickinson And Company	Methods and compositions for modulation of amplification efficiency
BR112017022117A2 (en)	2015-04-15	2018-07-10	Biosearch Technologies, Inc.	“Double suppressor probes”
US20210324461A1 (en)	2018-08-16	2021-10-21	Life Technologies Corporation	Reagents, mixtures, kits and methods for amplification of nucleic acids
US20240060117A1 (en)	2020-07-23	2024-02-22	Life Technologies Corporation	Energy transfer dye conjugates for use in biological assays
KR20230056685A (en)	2020-07-23	2023-04-27	라이프 테크놀로지스 코포레이션	Compositions, systems and methods for biological assays using dyes
CN117529529A (en)	2021-07-21	2024-02-06	生命技术公司	Azaindole cyanine dye, application and preparation method
CN117897454A (en)	2021-07-21	2024-04-16	生命技术公司	Dibenzoxanthene quencher, use and preparation method

Citations (5)

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Publication number	Priority date	Publication date	Assignee	Title
WO1986002929A1 (en)*	1984-11-08	1986-05-22	Life Technologies, Inc.	Nucleotide analogs for nucleic acid labeling and detection
WO1986004816A1 (en)*	1985-02-18	1986-08-28	Teijin Limited	Preparation of 5-fluoro-2'-deoxyuridine esters for intraarterial administration
EP0198207A1 (en)*	1985-03-21	1986-10-22	Molecular Diagnostics, Inc.	Specific iodination of nucleic acid
EP0242264A1 (en)*	1986-04-04	1987-10-21	Institut Pasteur	Non-radioactive, single-stranded labelled probe, method for its production and method of detecting a target nucleotide sequence using this probe
WO1988010264A1 (en)*	1987-06-24	1988-12-29	Howard Florey Institute Of Experimental Physiology	Nucleoside derivatives

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US4599303A (en)*	1983-12-12	1986-07-08	Hri Associates, Inc.	Nucleic acid hybridization assay employing probes crosslinkable to target sequences
US4950768A (en)*	1984-01-16	1990-08-21	Cronyn Marshall W	Cyclic disulfonic ester cross-linking compounds
EP0267996A1 (en)*	1986-11-20	1988-05-25	Tamir Biotechnology Ltd.	New nucleotide derivatives
US4837311A (en)*	1987-06-22	1989-06-06	Hoffman-La Roche Inc.	Anti-retroviral compounds

1990
- 1990-05-15EPEP19900908844patent/EP0472648A4/ennot_activeWithdrawn
- 1990-05-15JPJP2508242Apatent/JPH04507402A/enactivePending
- 1990-05-15WOPCT/US1990/002740patent/WO1990014353A1/ennot_activeApplication Discontinuation

Patent Citations (5)

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Publication number	Priority date	Publication date	Assignee	Title
WO1986002929A1 (en)*	1984-11-08	1986-05-22	Life Technologies, Inc.	Nucleotide analogs for nucleic acid labeling and detection
WO1986004816A1 (en)*	1985-02-18	1986-08-28	Teijin Limited	Preparation of 5-fluoro-2'-deoxyuridine esters for intraarterial administration
EP0198207A1 (en)*	1985-03-21	1986-10-22	Molecular Diagnostics, Inc.	Specific iodination of nucleic acid
EP0242264A1 (en)*	1986-04-04	1987-10-21	Institut Pasteur	Non-radioactive, single-stranded labelled probe, method for its production and method of detecting a target nucleotide sequence using this probe
WO1988010264A1 (en)*	1987-06-24	1988-12-29	Howard Florey Institute Of Experimental Physiology	Nucleoside derivatives

Non-Patent Citations (5)

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Title
CHEMICAL ABSTRACTS, vol. 92, no. 21, 26th May 1980, page 20, abstract no. 174218s, Columbus, Ohio, US; E. DE CLERCQ et al.: "Antiviral activity of 5-[(methylthio)methyl]-2'deoxyuridine and other 5-substituted 2'-deoxyuridines", & BIOCHEM. PHARMACOL. 1979, 28(22), 3249-54*
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 111, no. 18, 30th August 1989, pages 7226-7232, American Chemical Society; J. TELSER et al.: "DNA duplexes covalently labeled at two sites: synthesis and characterization by steady-state and time-resolved optical spectroscopies"*
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 111, no. 22, 25th October 1989, pages 8517-8519, American Chemical Society; R.B. MEYER et al.: "Efficient, specific cross-linking and cleavage of DNA by stable, synthetic complementary oligodeoxynucleotides"*
NUCLEIC ACIDS RESEARCH, vol. 15, no. 16, 25th August 1987, pages 6455-6467, IRL Press Ltd, Oxford, GB; K.J. GIBSON et al.: "synthesis and application of derivatizable oligonucleotides"*
See also references ofWO9014353A1*

Also Published As

Publication number	Publication date
WO1990014353A1 (en)	1990-11-29
EP0472648A1 (en)	1992-03-04
JPH04507402A (en)	1992-12-24

Publication	Publication Date	Title
EP0472648A4 (en)	1992-09-16	Crosslinking oligonucleotides
ZA904718B (en)	1991-06-26	Catalysts
GB9027137D0 (en)	1991-02-06	Processes
GB9025087D0 (en)	1991-01-02	Polymers
GB9016782D0 (en)	1990-09-12	Catalysts
GB8906697D0 (en)	1989-05-10	Numbers
EP0405787A3 (en)	1991-11-06	Catalysts
GB9013077D0 (en)	1990-08-01	Catalysts
GB8919104D0 (en)	1989-10-04	Automotive
AU107525S (en)	1990-05-04	Automotive weathershield
GB8913962D0 (en)	1989-08-02	Polymers
GB8926525D0 (en)	1990-01-10	Polymers
GB8915396D0 (en)	1989-08-23	Polymers
GB8920929D0 (en)	1989-11-01	Polymers
GB8924123D0 (en)	1989-12-13	Polymers
CU22023A1 (en)	1993-04-30	Equipo desmedulador de bagazo y otras fibras vegetales
AU105950S (en)	1989-11-14	Dashboard
ZA908330B (en)	1991-11-27	Steps
GB8926634D0 (en)	1990-01-17	Polynucleotides
GB8907653D0 (en)	1989-05-17	Polynucleotides
GB8907655D0 (en)	1989-05-17	Polynucleotides
GB8926382D0 (en)	1990-01-10	Catalysts
IE890423L (en)	1989-08-12	Catalysts
ZA906146B (en)	1991-06-26	Catalysts
GB8914576D0 (en)	1989-08-16	Catalysts

Legal Events

Date	Code	Title	Description
1992-01-17	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text:ORIGINAL CODE: 0009012
1992-03-04	17P	Request for examination filed	Effective date:19911113
1992-03-04	AK	Designated contracting states	Kind code of ref document:A1 Designated state(s):AT BE CH DE DK ES FR GB IT LI LU NL SE
1992-09-16	A4	Supplementary search report drawn up and despatched	Effective date:19920729
1992-09-16	AK	Designated contracting states	Kind code of ref document:A4 Designated state(s):AT BE CH DE DK ES FR GB IT LI LU NL SE
1997-01-29	17Q	First examination report despatched	Effective date:19961212
1999-06-11	STAA	Information on the status of an ep patent application or granted ep patent	Free format text:STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
1999-07-28	18D	Application deemed to be withdrawn	Effective date:19981107

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