CN85105522A - The making of dry-keeping macropathological specimens and preservation - Google Patents

Abstract

The making of dry-keeping macropathological specimens and preservation belong to the preservation technology of general pathology tissue specimen.Although it is varied to make and preserve the method for sample, all generally preserve with soaking liquid storage.With soaking the sample that liquid storage is preserved, preserve, transport, move, carry all inconvenient.And the present invention is after the general pathology sample is handled according to a conventional method, immerse carry out preservative treatment in 37-40 ℃ the impregnating fluid (Fa-84726) after, pack into to seal up for safekeeping and done preservation in the container.Entire making process can shorten to 3-6 days, the sample lovely luster, and form is true to nature, and antiseptic power is strong, saves material, and cost is low, preserves, transports, carries etc. all more convenient.

Description

The making of dry-keeping macropathological specimens and preservation

The invention belongs to the preservation technology of general pathology tissue specimen.It is not only applicable to the preservation of general Pathologic specimen, can also preserve the directive significance important to preserving of corpse and zoological specimens as dissec.

The demonstration sample of pathological tissue is the most valuable material in the work of pathological anatomy teaching research.It is good that sample is preserved, and must be noted that following technical finesse formality:

1. specimen sampling is healed better fresh.

2. behind the sample extraction, avoiding violence pincers tweezer as far as possible, and should be revised immediately, to thicker sample, should do once to cut with long steel knife, make tangent plane level and smooth, be good to lose tool marks.

3. fresh specimens is avoided and is washed with water, meets liquid and can wipe away with dried cloth when too much, slightly washes with physiological saline in case of necessity.

4. the sample vessel should use wide mouth that the flat cylinder of lid is arranged as far as possible, and specification than the big 10-20 of sample volume doubly.

5. sample injects vessel with fixer earlier fixedly the time, just can put into sample, and rebasing or separate with cotton, in order to avoid be close between sample and vessel or the sample, if meet floating sample, also covers exposed portions serve with the cotton libation at an ancient wedding ceremony fixer that wets, in order to avoid air-dry.

6. various

The device structure is different, must handle respectively by different characteristics.

The making and the preservation of primary colors general pathology sample, generally be that the process routine techniques is handled the ready Pathologic specimen of formality, place the container that fills fixer, look its sample size, fix through 1-15 days (even longer), the extruding sample if no pale red or turbid liquid flow out from otch, illustrates that promptly sample fixes; The sample that fixes is washed about 12-24 hour (with the increase and decrease as one feels fit of sample size) with flowing water; After sample is washed, put into 95% or 85-95% alcohol be dipped to sample primary colors degree of reverting to, big sample also should not surpass 2 hours, in order to avoid fade; Sample behind the secondary color is with steaming

Alcohol is removed in washing, and can pack into fills in the glass container that soaks liquid storage (preservation liquid), soak to preserve used fixer, secondary color alcohol and to soak the composition of liquid storage (preservation liquid) roughly as follows in said process:

1.KaiSellinShi liquid soaks the method for depositing:

(selecting from pathological tissue preparation of specimen technology)

First liquid (fixing):

200 milliliters of formalin

100 milliliters in running water

Potassium acetate 30 grams

Potassium nitrate 30 grams

Second liquid (secondary color): 95% alcohol (consumption was to soak sample degree of being)

The 3rd liquid (soak and deposit):

200 milliliters of glycerine

Steam

1000 milliliters in water

Potassium acetate 100 grams

Thymol 2.5 grams

2 magnesium sulfate mixed solutions soak the method for depositing:

First liquid (fixing)

100 milliliters of formalin

Sodium acetate 50 grams

Steam

1000 milliliters in water or cold boiling water

Second liquid (secondary color) 85-95% alcohol

The 3rd liquid (soak and deposit)

Magnesium sulfate 100 grams

Magnesium acetate 50-80 gram

Steam

1000 milliliters in water

Thymol or camphor are a small amount of

3. glycerine, potassium acetate mixed liquor soak the method for depositing:

(selecting from megasoma preparation of specimen method)

First liquid (fixing): 10% formaldehyde (formalin)

Second liquid (color reversion): 95% alcohol

The 3rd liquid (soak and deposit):

Potassium acetate 300 grams

Glycerine 500 grams

Steam1000 milliliters in water

Thymol 0.5 gram

Although it is varied to make and preserve the method for primary colors general pathology sample, but be all with soaking liquid storage and soak preservation, equal inconvenience is moved, is carried in this sample, storage, transportation of preserving with preservation liquid, and the preservation of sample nature color, can not obtain safety assurance only according to preserved material.Preserved material uses certain hour (as a year) that need are renewed liquid.

In order to overcome above-mentioned shortcoming, save raw material, shorten preparation of specimen's time, the present invention studies the dried store method of successful primary colors general pathology sample, promptly need not soak liquid storage and preserve sample.

Technical characterictic of the present invention is: the fixer that fixed preparation is used is that its cost of material is cheap by underground phreatic water, common salt and the formulated micro alkaline solution of formaldehyde (formalin).Sample behind the alcohol secondary color puts into that impregnating fluid permeates, anticorrosion, figuration is handled again.After handling well, move into again and under 37 ℃, do moisture content forfeiture in the insulating box and handle, the loss of water part in its sample can be sealed up for safekeeping, go through 24-72 hour in the spacious laboratory that is placed on ventilation when hot and also can.The sample of handling well more than the warp, put into clean, dry and the container of sealing up for safekeeping not doing to disinfect do to do is preserved, this seals container up for safekeeping can be that glass or lucite are made, the container flap should be with adhesive plaster, transparent adhesive tape or the sealing of envelope bottle glue, airtight preservation, impregnating fluid is to adopt the gluey liquid of Fa-84726 macromolecule, and during preservative treatment, the impregnating fluid temperature should be controlled between 37-40 ℃.

The making of dry-keeping macropathological specimens and preservation, can be undertaken by following step:

One, sample is fixed:

Ready fresh Pathologic specimen places in the following fixer after the technical finesse routinely:

10 milliliters in 38-40% formaldehyde (formalin)

100 milliliters of phreatic waters

Common salt 5 grams

During preparation said fixing liquid, should treat that sodium chloride fully dissolves after, add formaldehyde (formalin) solution again, to reduce the formaldehyde volatilization, seal so container must be the ground lid.Fixedly the container size of usefulness should be suitable, the fixer consumption is 5-10 a times of sample volume, set time notes being controlled at 1-3 days, big sample also is no more than 3 days, set time is long, can make the acid increase of sample tissue, can not make acid haemoglobin change alkaline haemoglobin into well, influence primary colors and recover to cause alcohol.Whether inspection specimen fixes.(because if having pale red or muddy liquid to flow out explanation from otch from otch or tangent plane during with hand extruding sample does not fix, the sample that does not fix will corrupt, rotten, variable color.) sample can carry out the flowing water flushing after fixing.

Two, flowing water flushing and alcohol secondary color:

Fixing sample completely with flowing water flushing 12-24 hour, is washed till till the complete obiteration of formalin smell.(push sample with hand, the liquid that flows out from otch is if there is the formalin smell, variable color in the time of will influence in alcohol secondary color and preservation).Purging method: sebific duct one is terminated on the tap, and the other end is with weight, makes it to be sunken to container bottom, allow water washing from bottom to top, washed sample takes out and uses the gauze suck dry moisture, inserts by (the most handy alcohol more than 90%) in the 80-90% alcohol, takes out when primary colors recovers.

The amount of the used alcohol of secondary color, the 6-7 that is generally the sample volume doubly or more, secondary color time length is indefinite, notes observing, with original color restoration degree of being, overlong time instead disappears the primary colors that has recovered to take off, and gets final product in about about 1 hour in the alcohol more than 90% usually.During with the alcohol secondary color, should in the transparent vessel of ground lid sealing, carry out, so that observe and reduce the volatilization of alcohol.Observe secondary color, carry out under the natural daylight by day, get rid of the interference of any artificial light, as far as possible in order to avoid distortion.

Three, set up glass supporter to the good sample of secondary color.

Easy to operate for anticorrosion process, set up glass supporter to the sample that secondary color is good.Install before the sample, used large size suture needle, a ball of string should be soaked in sterilization in 75% alcohol in advance, uses white line, in order to avoid fade, used glass supporter, sharp edges can be cleaned, dry standby with the emery wheel polishing again, can not sterilize.During installation, think better of the sightingpiston of abundant exposure sample, install and want solid reliable.

Four, will set up the sample of support, put into that impregnating fluid (Fa-84726 macromolecule gluey liquid) permeates, figuration, preservative treatment.

When the impregnating fluid temperature is controlled at 37-40 ℃, rack-mount sample is immersed in the impregnating fluid fully the lid of covered container, move to then in 37 ℃ the insulating box, this thickness and size of sighting target gone through 1-5 hour or through night, so that infiltration, anticorrosion, figuration.

Should carry out lentamente when from impregnating fluid, taking out sample, after taking out, sample stops slightly a moment, allow the impregnating fluid drip in container, make its tiltedly upright surplus elsewhere then, allow its too much impregnating fluid in surface continue to wander, drop on the following paper, can open in the laboratory that is placed on ventilation summer, goes through 24-72 hour, should insert winter in 37 ℃ of incubators, make its moisture content forfeiture a part of, can seal up for safekeeping.Make loss of water and handle, can make the film figuration and the antisepsis of impregnating fluid better, but loss of water is too much, can cause sample distortion, variable color, notice that therefore the control calorstat temperature can not be too high, note observing.

If the primary colors of sample is not ideal enough after above-mentioned processing, how, impregnating fluid fades due to the acidity because of being, sample can be put into once again alcohol secondary color again.

Five, preserve:

Setting sample after above-mentioned processing can be sealed up for safekeeping in glass specimen jar or lucite specimen jar, is done preservation.

The container of sealing sample up for safekeeping should clean, transparent, available washing powder, well water or running water clean, spacious being placed in the laboratory dried, and need not sterilize, and do not adorn preservation liquid, promptly can be used to preserve sample.

The support of slapping sample pack into seal container up for safekeeping after, under the situation of moderate length, with absorbent cotton, causing that sample is firm is degree in the upper/lower terminal pad.With adhesive plaster, adhesive tape or envelope bottle glue and so on sealing specimen jar flap, the year, month, day of putting on making gets final product then, and entire making process can shorten to 3-6 days.

Claims (8)

1, the making of dry-keeping macropathological specimens and preservation, be that Pathologic specimen that desire is preserved is after the technical finesse formality is handled routinely, place fixer to fix, wash with flowing water then, again to be preserved behind the alcohol secondary color, feature of the present invention is: fixer is by underground phreatic water, common salt and formaldehyde (formalin) are formulated, the sample that fixes is behind the alcohol secondary color, be installed on the glass supporter, permeate in the impregnating fluid of immersion uniform temperature, anticorrosion and figuration, from impregnating fluid, take out and put into cleaning after sample remakes moisture content forfeiture processing, transparent, that dries seals up for safekeeping in the container, and sealing specimen jar flap is done to do and preserved.

2, according to the described method of claim 1, it is characterized in that: the consisting of of fixer:

10 milliliters in 38-40% formaldehyde (formalin)

100 milliliters of underground phreatic waters

Common salt 5 grams

3, according to the described method of claim 1, it is characterized in that: impregnating fluid is the gluey liquid of Fa-84726 macromolecule.

4, according to the described method of claim 1, it is characterized in that: the temperature of impregnating fluid should be controlled between 37-40 ℃.

5, according to the described method of claim 1, it is characterized in that: the sample of being made by glass or lucite is sealed container up for safekeeping and only need be cleaned, dry, and needn't disinfect.

6, according to claim 1 and 5 described methods, it is characterized in that: seal up for safekeeping not adorn in the container of sample and soak liquid storage (preservation liquid), the sample of handling well the back of packing into is done to do and is preserved.

7, according to the described method of claim 1, it is characterized in that: sealing specimen jar flap can be used adhesive plaster, adhesive tape or envelope bottle glue etc.

8, method according to claim 1 is characterized in that: when doing moisture content forfeiture and handling, sample should be inserted winter in 37 ℃ the insulating box and carry out, can open in the laboratory that is placed on ventilation when hot and go through 24-72 hour summer.