技术领域Technical Field
本申请涉及电刺激器的技术领域，尤其是涉及一种细胞电刺激器。The present application relates to the technical field of electrical stimulators, and in particular to a cell electrical stimulator.
背景技术Background technique
在进行动物立体细胞的实验中，由于电刺激信号具有促进细胞的增殖及分化，改变细胞的力学特性，可以起到促进组织的生长的作用，因此，电刺激已经被广泛运用于医学研究、诊断、治疗及功能恢复中。研究表明，在适当的电刺激信号下，细胞的生长速度与再生能力可以得到显著地增强，进而可以促进组织的生长、伤口的愈合，在一定程度上证明了电刺激对于细胞的影响。In animal three-dimensional cell experiments, electrical stimulation has been widely used in medical research, diagnosis, treatment and functional recovery because electrical stimulation signals can promote cell proliferation and differentiation, change the mechanical properties of cells, and promote tissue growth. Studies have shown that under appropriate electrical stimulation signals, the growth rate and regeneration ability of cells can be significantly enhanced, which can promote tissue growth and wound healing, proving the influence of electrical stimulation on cells to a certain extent.
细胞电刺激器是为细胞生长提供电刺激的最基本的工具。细胞电刺激器的研制为研究细胞迁移、黏附、分化、代谢能力的变化机制以及电信号对人体组织生长的作用机制，提供了有利工具和良好方法。细胞电刺激器为定量、定时研究电刺激对于细胞的影响，以进一步得出电对于细胞乃至人体的作用机制，提供了有利工具、条件和良好方法,因此研制细胞培养电刺激器具有重要的临床意义和实用价值。The cell electrical stimulator is the most basic tool for providing electrical stimulation for cell growth. The development of the cell electrical stimulator provides a favorable tool and a good method for studying the changing mechanisms of cell migration, adhesion, differentiation, and metabolic capacity, as well as the mechanism of action of electrical signals on human tissue growth. The cell electrical stimulator provides a favorable tool, conditions, and a good method for quantitatively and regularly studying the effects of electrical stimulation on cells, in order to further derive the mechanism of action of electricity on cells and even the human body. Therefore, the development of the cell culture electrical stimulator has important clinical significance and practical value.
细胞刺激所用的最大电场强度为1V/mm，激信号的频率不超过50kHz，现在研究中使用的细胞刺激器大致可分为三类:(1)将交流电转换为特定直流电，通过电流通断的方式进行细胞的刺激，该类刺激器参数调节不方便并且刺激波形不可调节；(2)使用常规信号发生器作为信号来源，直接对细胞进行刺激，该类信号发生器波形种类及功率受限，刺激强度无法精确控制；(3)根据实验需要研制的特定细胞刺激器，该类刺激器虽然能满足当前实验的需求，但不具有通用性，一旦刺激方案更改，定制的刺激器无法使用。The maximum electric field strength used for cell stimulation is 1V/mm, and the frequency of the stimulation signal does not exceed 50kHz. The cell stimulators currently used in research can be roughly divided into three categories: (1) converting alternating current into a specific direct current and stimulating cells by switching the current on and off. The parameters of this type of stimulator are not convenient to adjust and the stimulation waveform cannot be adjusted; (2) using a conventional signal generator as a signal source to directly stimulate cells. The waveform types and power of this type of signal generator are limited, and the stimulation intensity cannot be accurately controlled; (3) specific cell stimulators developed according to experimental needs. Although this type of stimulator can meet the needs of current experiments, it is not universal. Once the stimulation scheme is changed, the customized stimulator cannot be used.
探索电刺激在生物医学中的应用引起了人们极大的兴趣，电刺激细胞实验研究方面取得的进展，了解电刺激如何影响细胞力学特性和生命活动对研发疾病诊疗新技术具有参考意义，然而电刺激作用于细胞的机制理论尚不完善，距离应用于实际治疗还需要经过大量实验，需要设置各种刺激参数进一步探究电刺激对实验的影响。目前研究中使用的细胞刺激器缺点主要有：Exploring the application of electrical stimulation in biomedicine has aroused great interest. The progress made in the experimental research of electrical stimulation cells and understanding how electrical stimulation affects the mechanical properties and life activities of cells are of reference significance for the development of new technologies for disease diagnosis and treatment. However, the theoretical mechanism of electrical stimulation on cells is still imperfect, and it will take a lot of experiments to be applied to actual treatment. Various stimulation parameters need to be set to further explore the impact of electrical stimulation on experiments. The main disadvantages of the cell stimulators currently used in research are:
1、部分细胞电刺激器仅将交流电转换成直流电，通过电流通断的方式进行细胞的刺激，该类刺激器参数调节不方便；1. Some cell electrical stimulators only convert alternating current into direct current and stimulate cells by switching the current on and off. The parameters of such stimulators are inconvenient to adjust;
2、根据实验方案要求定制细胞电刺激器，虽能满足本次实验，但不具有通用性，一旦刺激方案更改，定制的刺激器无法使用；2. Customized cell electrical stimulators according to the experimental plan can meet the requirements of this experiment, but they are not universal. Once the stimulation plan is changed, the customized stimulator cannot be used;
3、细胞培养箱在实验过程中为封闭环境，而大多数细胞电刺激器需要电源线或者数据线的连接，无法满足封闭要求；3. The cell culture incubator is a closed environment during the experiment, and most cell electrical stimulators require the connection of power cords or data cables, which cannot meet the closed requirements;
4、多数细胞电刺激器只满足普通防水要求，细胞培养箱密封且有液体，细胞电刺激器有受潮短路甚至损坏的风险。4. Most cell electrical stimulators only meet general waterproof requirements. The cell culture incubator is sealed and contains liquid, so the cell electrical stimulator is at risk of short circuit or even damage due to moisture.
实用新型内容Utility Model Content
为了解决现有技术存在的问题，本申请提供了一种细胞电刺激器。In order to solve the problems existing in the prior art, the present application provides a cell electrical stimulator.
为了实现上述目的，本申请采用如下的技术方案：In order to achieve the above objectives, this application adopts the following technical solutions:
一种细胞电刺激器，包括防水外壳、细胞电刺激器电路板以及可充电电池；A cell electrical stimulator comprises a waterproof housing, a cell electrical stimulator circuit board and a rechargeable battery;
所述防水外壳上设有防水天线、防水刺激电极接口以及防水充电接口；The waterproof housing is provided with a waterproof antenna, a waterproof stimulation electrode interface and a waterproof charging interface;
所述细胞电刺激器电路板包括电源管理充电模块、第一主控模块、刺激输出模块以及第一无线传输模块；The cell electrical stimulator circuit board includes a power management charging module, a first main control module, a stimulation output module and a first wireless transmission module;
所述电源管理充电模块与可充电电池、第一主控模块、刺激输出模块以及第一无线传输模块连接，实现供电功能；所述第一无线传输模块与防水天线以及第一主控模块连接，实现刺激信号接收功能；所述刺激输出模块与第一主控模块连接，且所述刺激输出模块通过防水刺激电极接口将第一主控模块输出的刺激信号放大并传输至防水刺激电极接口外接的刺激电极，实现对细胞的电刺激。The power management charging module is connected to the rechargeable battery, the first main control module, the stimulation output module and the first wireless transmission module to realize the power supply function; the first wireless transmission module is connected to the waterproof antenna and the first main control module to realize the stimulation signal receiving function; the stimulation output module is connected to the first main control module, and the stimulation output module amplifies the stimulation signal output by the first main control module through the waterproof stimulation electrode interface and transmits it to the stimulation electrode externally connected to the waterproof stimulation electrode interface to realize electrical stimulation of the cells.
优选的，所述刺激输出模块包括至少一个放大子模块以及至少一个恒流或恒压输出子模块，所述放大子模块与对应的恒流或恒压输出子模块连接，所述第一主控模块输出的刺激信号经放大子模块处理后传输至对应的恒流或恒压输出子模块；所述恒流或恒压输出子模块与防水刺激电极接口连接，以输出刺激信号。Preferably, the stimulation output module includes at least one amplifying submodule and at least one constant current or constant voltage output submodule, the amplifying submodule is connected to the corresponding constant current or constant voltage output submodule, and the stimulation signal output by the first main control module is processed by the amplifying submodule and transmitted to the corresponding constant current or constant voltage output submodule; the constant current or constant voltage output submodule is connected to the waterproof stimulation electrode interface to output the stimulation signal.
优选的，所述防水外壳包括壳体、前盖以及后盖，所述前盖和后盖安装在壳体的相对两侧，所述前盖上安装有开关和防水刺激电极接口，所述后盖上安装有防水天线和防水充电接口，所述细胞电刺激器电路板和可充电电池安装在壳体内，所述前盖和后盖与壳体之间设置有防水的密封结构。Preferably, the waterproof housing includes a shell, a front cover and a back cover, the front cover and the back cover are installed on opposite sides of the shell, the front cover is installed with a switch and a waterproof stimulation electrode interface, the back cover is installed with a waterproof antenna and a waterproof charging interface, the cell electrical stimulator circuit board and the rechargeable battery are installed in the shell, and a waterproof sealing structure is arranged between the front cover, the back cover and the shell.
优选的，所述前盖和后盖上设置有对应的卡接板，所述细胞电刺激器电路板的两端分别与前盖和后盖上的卡接板卡接配合。Preferably, corresponding snap-in plates are provided on the front cover and the rear cover, and two ends of the cell electrical stimulator circuit board are snap-fitted with the snap-in plates on the front cover and the rear cover respectively.
优选的，所述壳体的底部设置有限位结构，所述限位结构用于对可充电电池进行限位。Preferably, a limiting structure is provided at the bottom of the shell, and the limiting structure is used to limit the rechargeable battery.
优选的，述刺激输出模块为AD8531ARTZ芯片。Preferably, the stimulation output module is an AD8531ARTZ chip.
优选的，所述第一主控模块为STM32L151RCT6芯片。Preferably, the first main control module is a STM32L151RCT6 chip.
优选的，所述第一无线传输模块为NRF24L01P芯片。Preferably, the first wireless transmission module is an NRF24L01P chip.
优选的，所述可充电电池为955465锂电池。Preferably, the rechargeable battery is a 955465 lithium battery.
优选的，所述防水外壳可以是塑料、铝合金或者两种兼有的材质，所述防水外壳连接处设有密封圈。Preferably, the waterproof shell can be made of plastic, aluminum alloy or a combination of the two, and a sealing ring is provided at the connection of the waterproof shell.
与现有技术相比，本申请具有以下有益效果：Compared with the prior art, this application has the following beneficial effects:
本申请第一无线传输模块接受外部设备，如计算机设备发送的刺激信号，将刺激信号传输至第一主控模块，然后第一主控模块将接受的刺激信号转化后输出至刺激输出模块，刺激输出模块接受至第一主控模块的刺激信号方法后，传输至刺激电极，即可实现对细胞的电刺激。通过设置第一无线传输模块，可接收不同的刺激参数，经过第一主控模块处理后转化为刺激输出模块可执行的刺激信号，实现细胞电刺激器可调节刺激参数的效果，且可根据实验方案要求定制细胞电刺激器，具有通用性，且本申请采用可充电电池，无需外接电源线，配合防水外壳，即可实现细胞电刺激器的防水功能，满足细胞培养箱的封闭要求，也使细胞电刺激器不易受潮而损坏。The first wireless transmission module of the present application receives a stimulation signal sent by an external device, such as a computer device, and transmits the stimulation signal to the first main control module. Then, the first main control module converts the received stimulation signal and outputs it to the stimulation output module. After the stimulation output module receives the stimulation signal from the first main control module, it transmits it to the stimulation electrode, thereby realizing electrical stimulation of the cells. By setting the first wireless transmission module, different stimulation parameters can be received, which are converted into stimulation signals executable by the stimulation output module after being processed by the first main control module, thereby realizing the effect that the stimulation parameters of the cell electrical stimulator can be adjusted, and the cell electrical stimulator can be customized according to the requirements of the experimental plan, which is universal. In addition, the present application adopts a rechargeable battery, does not require an external power cord, and cooperates with a waterproof shell to realize the waterproof function of the cell electrical stimulator, meet the sealing requirements of the cell culture box, and make the cell electrical stimulator not easily damaged by moisture.
附图说明BRIEF DESCRIPTION OF THE DRAWINGS
图1为本申请实施例的电路结构示意图；FIG1 is a schematic diagram of a circuit structure of an embodiment of the present application;
图2为本申请实施例的结构示意图；FIG2 is a schematic diagram of the structure of an embodiment of the present application;
图3为本申请实施例的爆炸示意图；FIG3 is an exploded schematic diagram of an embodiment of the present application;
图4为本申请实施例中电源管理充电模块的电路图；FIG4 is a circuit diagram of a power management charging module in an embodiment of the present application;
图5为本申请实施例中第一主控模块的电路图；FIG5 is a circuit diagram of a first main control module in an embodiment of the present application;
图6为本申请实施例中刺激输出模块的电路图；FIG6 is a circuit diagram of a stimulation output module in an embodiment of the present application;
图7为本申请实施例中第一无线传输模块的电路图。FIG. 7 is a circuit diagram of the first wireless transmission module in an embodiment of the present application.
图中：In the figure:
1、防水外壳；11、壳体；12、前盖；13、后盖；14、卡接板；15、限位结构；16、密封结构；2、细胞电刺激器电路板；21、电源管理充电模块；22、第一主控模块；23、刺激输出模块；24、第一无线传输模块；3、可充电电池；4、防水天线；5、防水刺激电极接口；6、防水充电接口；7、开关。1. Waterproof shell; 11. Shell; 12. Front cover; 13. Back cover; 14. Snap-in plate; 15. Limiting structure; 16. Sealing structure; 2. Cell electrical stimulator circuit board; 21. Power management charging module; 22. First main control module; 23. Stimulation output module; 24. First wireless transmission module; 3. Rechargeable battery; 4. Waterproof antenna; 5. Waterproof stimulation electrode interface; 6. Waterproof charging interface; 7. Switch.
具体实施方式Detailed ways
下面将结合本实用新型实施例中的附图，对本实用新型实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本实用新型一部分实施例，而不是全部的实施例。The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.
本申请公开了一种细胞电刺激器。The present application discloses a cell electrical stimulator.
如图1至图7所示，细胞电刺激器，包括防水外壳1、细胞电刺激器电路板2以及可充电电池3，细胞电刺激器电路板2以及可充电电池3安装于防水外壳1内。As shown in FIG. 1 to FIG. 7 , the cell electrical stimulator includes a waterproof housing 1 , a cell electrical stimulator circuit board 2 and a rechargeable battery 3 , and the cell electrical stimulator circuit board 2 and the rechargeable battery 3 are installed in the waterproof housing 1 .
具体的，在本实施例中，防水外壳1可以是塑料、铝合金或者两种兼有的材质，当然，在本申请的其他实施例中，防水外壳1也可以为其他，不与细胞培养皿中的营养液等反应的材料，在此不做赘述。Specifically, in this embodiment, the waterproof shell 1 can be made of plastic, aluminum alloy or a combination of the two. Of course, in other embodiments of the present application, the waterproof shell 1 can also be made of other materials that do not react with the nutrient solution in the cell culture dish, which will not be elaborated here.
在本实施例中，防水外壳1根据实际需求设置，防水外壳1的壳体11连接处设有密封结构16，保证防水等级至少达到IPX67，防水外壳1上配有防水天线4、防水刺激电极接口5、防水充电接口6。在本实施例中，防水外壳1的壳体11连接处理解为组成防水外壳1的至少两半壳体11之间的连接处和/或壳体11与外部其他设备连接的连接处；其中刺激电极接口通过连接线与用于刺激细胞的刺激电极相连，防水充电接口6用于通过连接带有充电线的电源适配器为细胞电刺激器充电。通过设置防水外壳1包裹细胞电刺激器电路板2以及可充电电池3，并在防水外壳1的壳体11的连接处设置有密封圈，使外部的水被阻挡在防水外壳1外侧，实现细胞电刺激器的防水功能。In this embodiment, the waterproof housing 1 is set according to actual needs, and a sealing structure 16 is provided at the connection of the shell 11 of the waterproof housing 1 to ensure that the waterproof level reaches at least IPX67. The waterproof housing 1 is equipped with a waterproof antenna 4, a waterproof stimulation electrode interface 5, and a waterproof charging interface 6. In this embodiment, the connection of the shell 11 of the waterproof housing 1 is interpreted as the connection between at least two half shells 11 constituting the waterproof housing 1 and/or the connection between the shell 11 and other external devices; wherein the stimulation electrode interface is connected to the stimulation electrode for stimulating cells through a connecting line, and the waterproof charging interface 6 is used to charge the cell electrical stimulator by connecting a power adapter with a charging line. By providing a waterproof housing 1 to wrap the cell electrical stimulator circuit board 2 and the rechargeable battery 3, and providing a sealing ring at the connection of the shell 11 of the waterproof housing 1, the external water is blocked outside the waterproof housing 1, thereby realizing the waterproof function of the cell electrical stimulator.
如图2和图3所示，具体的，防水外壳1设置为长方体状结构，防水结构包括壳体11、前盖12和后盖13。前盖12和后盖13通过螺钉可拆卸固定在壳体11的前后端部，前盖12上安装有开关7和防水刺激电极接口5，开关7和防水刺激电极接口5与前盖12密封连接，起到防水的作用。后盖13上安装有防水天线4和防水充电接口6，防水天线4和防水充电接口6与后盖13密封连接，起到防水的作用。细胞电刺激器电路板2和可充电电池3安装在壳体11内，前盖12和后盖13与壳体11之间安装有防水的密封结构16。防水外壳1的结构设置，保证了其防水等级至少达到IPX67。As shown in Figures 2 and 3, specifically, the waterproof housing 1 is configured as a rectangular parallelepiped structure, and the waterproof structure includes a housing 11, a front cover 12, and a rear cover 13. The front cover 12 and the rear cover 13 are detachably fixed to the front and rear ends of the housing 11 by screws, and the front cover 12 is provided with a switch 7 and a waterproof stimulation electrode interface 5, which are sealed and connected to the front cover 12 to achieve a waterproof effect. The rear cover 13 is provided with a waterproof antenna 4 and a waterproof charging interface 6, which are sealed and connected to the rear cover 13 to achieve a waterproof effect. The cell electrical stimulator circuit board 2 and the rechargeable battery 3 are installed in the housing 11, and a waterproof sealing structure 16 is installed between the front cover 12 and the rear cover 13 and the housing 11. The structural setting of the waterproof housing 1 ensures that its waterproof grade reaches at least IPX67.
上述密封结构16一般设置为橡胶圈或橡胶垫，为了便于密封结构16的安装，在前盖12和后盖13上开设有嵌槽，密封结构16安装在嵌槽内，前盖12和后盖13固定在壳体11上时，密封结构16实现对壳体11连接处的密封，起到防水的目的。The above-mentioned sealing structure 16 is generally set as a rubber ring or a rubber pad. In order to facilitate the installation of the sealing structure 16, an embedding groove is opened on the front cover 12 and the rear cover 13, and the sealing structure 16 is installed in the embedding groove. When the front cover 12 and the rear cover 13 are fixed on the shell 11, the sealing structure 16 realizes the sealing of the connection between the shell 11 and achieves the purpose of waterproofing.
为了便于前盖12、后盖13与壳体11的安装，在前盖12和后盖13上形成有定位结构，如在前盖12和后盖13上形成定位板，定位板与壳体11的内侧面贴合，使得前盖12、后盖13和壳体11上的安装孔位相对应，便于快速实现安装。同时，密封结构16套设在定位结构上，对密封结构16起到一定的限位功能。In order to facilitate the installation of the front cover 12, the rear cover 13 and the housing 11, a positioning structure is formed on the front cover 12 and the rear cover 13, such as a positioning plate formed on the front cover 12 and the rear cover 13, and the positioning plate is fitted with the inner side of the housing 11, so that the installation holes on the front cover 12, the rear cover 13 and the housing 11 correspond to each other, which is convenient for quick installation. At the same time, the sealing structure 16 is sleeved on the positioning structure, which plays a certain limiting function for the sealing structure 16.
在本实施例中，前盖12和后盖13的上部固定有对应的卡接板14，细胞电刺激器电路板2的两端分别与前盖12和后盖13上的卡接板14卡接配合。卡接板14设置为四个，两两为一组，两组卡接板14分别固定在前盖12和后盖13上，细胞电刺激器电路板2的四个角卡接在卡接板14上，实现对细胞电刺激器电路板2的稳定固定。In this embodiment, corresponding clamping plates 14 are fixed to the upper parts of the front cover 12 and the rear cover 13, and the two ends of the cell electrical stimulator circuit board 2 are respectively clamped and matched with the clamping plates 14 on the front cover 12 and the rear cover 13. Four clamping plates 14 are provided, two by two in a group, and two groups of clamping plates 14 are respectively fixed to the front cover 12 and the rear cover 13, and the four corners of the cell electrical stimulator circuit board 2 are clamped on the clamping plates 14, so as to achieve stable fixation of the cell electrical stimulator circuit board 2.
同样的，在壳体11的底部固定有限位结构15，限位结构15用于对可充电电池3进行限位。限位结构15为四个限位板，四个限位板形成了与可充电电池3相适配的限位腔，可充电电池3粘接在限位腔内。为使得可充电电池3稳定位于壳体11内，在前盖12和/或后盖13上固定有压板，压板与可充电电池3的表面贴合。Similarly, a limiting structure 15 is fixed at the bottom of the housing 11, and the limiting structure 15 is used to limit the rechargeable battery 3. The limiting structure 15 is four limiting plates, and the four limiting plates form a limiting cavity adapted to the rechargeable battery 3, and the rechargeable battery 3 is bonded in the limiting cavity. In order to stably position the rechargeable battery 3 in the housing 11, a pressure plate is fixed on the front cover 12 and/or the rear cover 13, and the pressure plate is in contact with the surface of the rechargeable battery 3.
如图1和图3所示，细胞电刺激器电路板2包括电源管理充电模块21、第一主控模块22、刺激输出模块23以及第一无线传输模块24，电源管理充电模块21与可充电电池3、第一主控模块22、刺激输出模块23以及第一无线传输模块24连接，实现供电功能；第一无线传输模块24与防水天线4以及第一主控模块22连接，实现刺激信号接收功能；刺激输出模块23与第一主控模块22连接，且刺激输出模块23通过防水刺激电极接口5将第一主控模块22输出的刺激信号放大并传输至防水刺激电极接口5外接的刺激电极，实现对细胞的电刺激。As shown in Figures 1 and 3, the cell electrical stimulator circuit board 2 includes a power management and charging module 21, a first main control module 22, a stimulation output module 23 and a first wireless transmission module 24. The power management and charging module 21 is connected to the rechargeable battery 3, the first main control module 22, the stimulation output module 23 and the first wireless transmission module 24 to realize the power supply function; the first wireless transmission module 24 is connected to the waterproof antenna 4 and the first main control module 22 to realize the stimulation signal receiving function; the stimulation output module 23 is connected to the first main control module 22, and the stimulation output module 23 amplifies the stimulation signal output by the first main control module 22 through the waterproof stimulation electrode interface 5 and transmits it to the stimulation electrode externally connected to the waterproof stimulation electrode interface 5 to realize electrical stimulation of the cells.
图4为电源管理充电模块21的电路图，具体的，电源管理充电模块21中，充电部分可以但不限于采用TP4056芯片，它是完整的单节锂离子电池采用的恒定电流/恒定电压线性充电芯片，适合于USB电源和适配器电源工作。低压差稳压器可以使用NCP163ASN330T1G。FIG4 is a circuit diagram of the power management charging module 21. Specifically, in the power management charging module 21, the charging part can be, but is not limited to, a TP4056 chip, which is a constant current/constant voltage linear charging chip used by a complete single-cell lithium-ion battery, suitable for USB power supply and adapter power supply. The low voltage dropout regulator can use NCP163ASN330T1G.
图5为第一主控模块22的电路图，第一主控模块22可以采用超低功耗高性能芯片STM32L151RCT6，-M3 32位RISC内核，使用1.8-3.6V电源。FIG5 is a circuit diagram of the first main control module 22. The first main control module 22 may use an ultra-low power consumption and high performance chip STM32L151RCT6. -M3 32-bit RISC core, using 1.8-3.6V power supply.
图6为刺激输出模块23的电路图，刺激输出模块23可以应用AD8531ARTZ，它是单通道轨到轨输入与输出、单电源放大器，具有250mA输出驱动电流。这种高输出电流特性使这些放大器特别适合驱动阻性或容性负载。可以实现多通道刺激输出，刺激输出包括恒压输出和恒流输出，输出电压可调，输出电流可调。FIG6 is a circuit diagram of the stimulation output module 23. The stimulation output module 23 may use the AD8531ARTZ, which is a single-channel rail-to-rail input and output, single-supply amplifier with a 250 mA output drive current. This high output current characteristic makes these amplifiers particularly suitable for driving resistive or capacitive loads. Multi-channel stimulation outputs may be realized, and the stimulation outputs include constant voltage output and constant current output, and the output voltage and output current are adjustable.
图7为第一无线传输模块24的电路图，第一无线传输模块24可以采用NRF24L01P，它是由NORDIC生产的工作在2.4GHz-2.5GHz的ISM频段的单片无线收发器芯片；第一无线传输模块24包括：频率发生器、增强型“SchockBurst”模式控制器、功率放大器、晶体振荡器、调制器和解调器，且第一无线传输模块24在1.9V-3.6V的电源范围。Figure 7 is a circuit diagram of the first wireless transmission module 24. The first wireless transmission module 24 can adopt NRF24L01P, which is a single-chip wireless transceiver chip produced by NORDIC and operates in the ISM frequency band of 2.4GHz-2.5GHz; the first wireless transmission module 24 includes: a frequency generator, an enhanced "SchockBurst" mode controller, a power amplifier, a crystal oscillator, a modulator and a demodulator, and the first wireless transmission module 24 is in the power supply range of 1.9V-3.6V.
当然，在本申请的其他实施例中，电源管理充电模块21、第一主控模块22、刺激输出模块23以及第一无线传输模块24均可以采用现有的可实现其功能的电路模块，在本申请中不做限定。Of course, in other embodiments of the present application, the power management charging module 21, the first main control module 22, the stimulation output module 23 and the first wireless transmission module 24 can all adopt existing circuit modules that can realize their functions, which is not limited in the present application.
可充电电池3的选择需要考虑刺激方案执行的时间，按照不低于10天的连续刺激强度设计，在通常情况下可以默认采用型号955465锂电池，尺寸9.5mm*54mm*65mm，1500mAh，额定电压3.7V，可满足大多数实验要求。可以预知的，根据实际实验要求，可充电电池3可选择其他现有的电池，在此不做赘述。The selection of the rechargeable battery 3 needs to consider the time of the stimulation program execution, and is designed according to a continuous stimulation intensity of not less than 10 days. Under normal circumstances, the default model 955465 lithium battery can be used, with a size of 9.5mm*54mm*65mm, 1500mAh, and a rated voltage of 3.7V, which can meet most experimental requirements. It can be predicted that according to the actual experimental requirements, the rechargeable battery 3 can select other existing batteries, which will not be described here.
在本实施例中，第一无线传输模块24接受外部设备，如计算机设备发送的刺激信号，将刺激信号传输至第一主控模块22，然后第一主控模块22将接受的刺激信号转化后输出至刺激输出模块23，刺激输出模块23接受至第一主控模块22的刺激信号方法后，传输至刺激电极，即可实现对细胞的电刺激。通过设置第一无线传输模块24，可接收不同的刺激参数，经过第一主控模块22处理后转化为刺激输出模块23可执行的刺激信号，实现细胞电刺激器可调节刺激参数的效果，且可根据实验方案要求定制细胞电刺激器，具有通用性，且本申请采用可充电电池3，无需外接电源线，配合防水外壳1，即可实现细胞电刺激器的防水功能，满足细胞培养箱的封闭要求，也使细胞电刺激器不易受潮而损坏。In this embodiment, the first wireless transmission module 24 receives a stimulation signal sent by an external device, such as a computer device, and transmits the stimulation signal to the first main control module 22. Then, the first main control module 22 converts the received stimulation signal and outputs it to the stimulation output module 23. After the stimulation output module 23 receives the stimulation signal from the first main control module 22, it transmits it to the stimulation electrode, thereby realizing electrical stimulation of the cells. By setting the first wireless transmission module 24, different stimulation parameters can be received, and after being processed by the first main control module 22, they are converted into stimulation signals executable by the stimulation output module 23, thereby realizing the effect that the cell electrical stimulator can adjust the stimulation parameters, and the cell electrical stimulator can be customized according to the requirements of the experimental plan, which has universality. In addition, the present application adopts a rechargeable battery 3, does not require an external power cord, and cooperates with the waterproof housing 1 to realize the waterproof function of the cell electrical stimulator, meet the sealing requirements of the cell culture box, and also make the cell electrical stimulator not easily damaged by moisture.
进一步地，刺激输出模块23包括至少一个放大子模块以及至少一个恒流或恒压输出子模块，放大子模块与对应的恒流或恒压输出子模块连接，第一主控模块22输出的刺激信号经放大子模块处理后传输至对应的恒流或恒压输出子模块；恒流或恒压输出子模块与防水刺激电极接口5连接，以输出刺激信号。刺激输出模块23，可根据实际的实验需求，输出电流或电压刺激信号，可满足多种实验要求，使本申请的细胞电刺激器更具备通用性。Furthermore, the stimulation output module 23 includes at least one amplifying submodule and at least one constant current or constant voltage output submodule, the amplifying submodule is connected to the corresponding constant current or constant voltage output submodule, the stimulation signal output by the first main control module 22 is processed by the amplifying submodule and transmitted to the corresponding constant current or constant voltage output submodule; the constant current or constant voltage output submodule is connected to the waterproof stimulation electrode interface 5 to output the stimulation signal. The stimulation output module 23 can output current or voltage stimulation signals according to actual experimental requirements, which can meet a variety of experimental requirements, making the cell electrical stimulator of the present application more versatile.
以上所述，仅为本实用新型较佳的具体实施方式，但本实用新型的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本实用新型揭露的技术范围内，根据本实用新型的技术方案及其实用新型构思加以等同替换或改变，都应涵盖在本实用新型的保护范围之内。The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent substitutions or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322845439.1UCN221460402U (en) | 2023-10-23 | 2023-10-23 | Cell electric stimulator |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322845439.1UCN221460402U (en) | 2023-10-23 | 2023-10-23 | Cell electric stimulator |
| Publication Number | Publication Date |
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| CN221460402Utrue CN221460402U (en) | 2024-08-02 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322845439.1UActiveCN221460402U (en) | 2023-10-23 | 2023-10-23 | Cell electric stimulator |
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| CN (1) | CN221460402U (en) |
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