Disclosure of Invention
In view of the above drawbacks, an object of the present utility model is to provide a novel cell co-culture dish which can satisfy the imaging requirements of a confocal microscope and is convenient for observation.
The utility model provides a cell co-culture dish which comprises a dish body with an open top, wherein an observation hole is formed in the bottom of the dish body, a glass sheet is arranged at the bottom of the observation hole, a plurality of supporting pieces are arranged in the dish body along the periphery of the observation hole, a cell climbing sheet can be placed on the supporting pieces, a gap is formed between the cell climbing sheet and the bottom of the dish body, and the gap is used for accommodating cultured cells.
Preferably, the support is triangular.
Preferably, the number of the supporting pieces is three, and the three supporting pieces are uniformly and alternately arranged along the periphery of the observation hole.
Preferably, the viewing aperture is circular.
Preferably, the diameter of the dish body is 35mm, and the diameter of the observation hole is 10mm.
Preferably, the dish cover is covered at the top opening of the dish body.
Preferably, the glass sheet has a thickness of 0.17mm.
The utility model has the advantages that the co-culture requirement is met and the imaging requirement of the confocal microscope is met by arranging the observation hole, the glass sheet and the supporting piece at the periphery of the observation hole at the bottom of the dish body. The cell culture dish has simple structure, is easy to produce and assemble, and can not damage cells in the use process.
Detailed Description
The following describes the embodiments of the present utility model in further detail with reference to the accompanying drawings. These embodiments are merely illustrative of the present utility model and are not intended to be limiting.
In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "top", "bottom", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.
As shown in fig. 1 and 2, the present utility model provides a cell co-culture dish, which comprises a dish body 1 with an open top, wherein an observation hole 4 is formed at the bottom of the dish body 1, a glass sheet 5 is arranged at the bottom of the observation hole 4, a plurality of supporting members 3 are arranged in the dish body 1 along the periphery of the observation hole 4, a cell climbing sheet 6 can be placed on the plurality of supporting members 3, and a gap 7 is formed between the cell climbing sheet and the bottom of the dish body 1, wherein the gap 7 is used for accommodating cultured cells.
Specifically, the dish body 1 is a hollow cylinder with the diameter of 35mm, the dish cover 2 is covered at the open top of the hollow cylinder, and the dish cover 2 is used for sealing the inside of the dish body 1, so that the influence of the external environment on cell culture is reduced. The observation hole 4 is a through hole, is circular and has a diameter of 10mm. The glass sheet 5 is fixed at the bottom of the dish body 1 and has a thickness of 0.17mm. Compared with the prior art, the utility model reduces the thickness of the bottom of a cell observation position and meets the optical requirement of confocal microscope observation by arranging the glass sheet 5 with better light transmittance.
In one embodiment of the utility model, the supports 3 are triangular in shape, three in number, and the three supports 3 are arranged at uniform, spaced intervals along the periphery of the viewing aperture 4. The cell climbing sheet 6 is a round glass sheet with the diameter of 10mm and the thickness of 0.17mm, and can be clamped at a height of 2mm from the bottom of the dish body 1 after being placed on the three supporting pieces 3. The experimenter can clamp the cell climbing sheet 6 through the tweezers to finish placing and removing, the probability of damaging cells is greatly reduced.
In the experiment, as shown in fig. 3, the cell climbing sheet 6 is placed in a common cell culture dish, the cell A is planted on the cell climbing sheet 6, the cell B is planted in the cell co-culture dish of the utility model, and after the cell A is completely attached to the cell climbing sheet 6 and the cell B is completely attached to the inner wall of the dish body 1, the required treatment of the experiment is respectively carried out. The cell slide 6 is then clamped off and placed on the support 3 in the dish 1 as shown in fig. 4, and the culture solution is added to perform co-culture of the two cells. Due to the gap 7, the cell climbing sheet 6 does not damage the cells B located on the inner wall of the dish body 1. Two cells can be imaged directly after the end of the experiment using confocal microscopy.
The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that modifications and substitutions can be made by those skilled in the art without departing from the technical principles of the present utility model, and these modifications and substitutions should also be considered as being within the scope of the present utility model.