CN218596435U - An automatic blower for subculture - Google Patents

Abstract

The utility model discloses an automatic ware of beating of blowing of subculture belongs to cell culture apparatus technical field. Including U type buckle, the grab handle, a switch, a support, buffer, speed regulator knob and telescoping device, U type buckle closure is on the pipettor lateral wall, U type buckle tip is equipped with keeps off the ring, U type buckle lateral wall fixedly connected with grab handle, the leg joint telescoping device is passed through to U type buckle rear end, the flexible rod end of telescoping device and the button of pipettor are on same axis, the flexible rod end of telescoping device is equipped with the buffer of roof pressure pipettor button, be equipped with battery and telescoping device's driver in the grab handle, be equipped with the speed governing knob on the telescoping device's the driver, be equipped with the switch of being connected in battery and telescoping device's driver respectively on the grab handle. Use the automation that this instrument can invariable dynamics, stroke to blow and beat the cell, improve the stability of transmission operation, make the operator more laborsaving, prevent the cell damage.

Description

Translated fromChinese

一种传代培养自动吹打器An automatic blower for subculture

技术领域technical field

本实用新型涉及一种传代培养自动吹打器，属于细胞培养用具技术领域。The utility model relates to an automatic blower for subculture, which belongs to the technical field of cell culture equipment.

背景技术Background technique

原代细胞培养成功以后，需要进行分离培养，否则细胞会因生存空间不足或密度过大、营养障碍，影响细胞生长。细胞由原培养瓶内分离稀释后传到新的培养瓶中培养的过程称之为传代培养。After the primary cells are successfully cultured, they need to be separated and cultured, otherwise the cells will be affected by insufficient living space, excessive density, and nutritional barriers, which will affect the growth of the cells. The process in which cells are separated and diluted from the original culture bottle and then transferred to a new culture bottle is called subculture.

传代培养的操作中，首先要用胰蛋白酶消化，使细胞从瓶壁脱落，然后使用吸管吸取瓶内培养液，并按顺序反复轻轻吹打瓶壁细胞，使之从瓶壁脱离形成细胞悬液。吹打时动作要轻柔，以防用力过猛损伤细胞。In the operation of subculture, first digest with trypsin to make the cells fall off the bottle wall, then use a pipette to suck up the culture medium in the bottle, and gently blow the bottle wall cells in order to make them detach from the bottle wall to form a cell suspension . Be gentle when blowing to prevent excessive force from damaging the cells.

吹打细胞，说起来只有简简单单四个字，但如果吹打掌握不好，细胞很容易受到机械损伤和剪切力影响，导致细胞受损严重、状态变差或者直接死亡。如果吹打不充分，将导致细胞抱团，将影响细胞数量。机械损伤主要是指枪头刮擦、细胞间碰撞导致的细胞损伤。Blowing cells is only a simple word, but if blowing is not mastered well, cells are easily affected by mechanical damage and shear force, resulting in severe damage, deterioration of state or direct death of cells. Insufficient pipetting will lead to cell clumping and affect the number of cells. Mechanical damage mainly refers to the cell damage caused by the scratching of the tip of the pipette and the collision between cells.

尤其是枪头与细胞层的接触甚至研磨，会导致细胞直接死亡。做实验时需要靠主动意识控制枪头与培养瓶底间距，避免枪头直接接触瓶底，同时还需要实验人员控制吹打的力度，防止用力过猛。In particular, the contact or even grinding of the pipette tip with the cell layer will lead to direct cell death. When doing experiments, it is necessary to rely on active consciousness to control the distance between the tip of the pipette and the bottom of the culture bottle to avoid direct contact of the pipette head with the bottom of the bottle. At the same time, the experimenter needs to control the strength of blowing to prevent excessive force.

目前实验中主要采用以下几种方式进行吹打细胞：At present, the following methods are mainly used to blow and beat cells in the experiment:

1.胶头滴管：因其需要使用前将玻璃滴管进行灭菌处理，若灭菌不充分容易造成细胞污染导致实验失败；而使用一次性滴管，其存在胶头老化吸力不够，细胞抱团或贴壁细胞没有脱离瓶壁的情况，使细胞数量减少，所以目前很少采用。1. Plastic tip dropper: Because the glass dropper needs to be sterilized before use, if the sterilization is not sufficient, it will easily cause cell contamination and cause the experiment to fail; while using a disposable dropper, the rubber tip is aging and the suction is not enough, and the cells Clusters or adherent cells do not detach from the bottle wall, which reduces the number of cells, so it is rarely used at present.

2.一次性吸管：这类型的吸管不易握持，在吹打细胞时很难控制，容易碰伤细胞层；而且吸头比较短，容易导致在吹打细胞时出现带有细胞的培养液残留到吸管头部，增加细胞因挤压过度导致损伤甚至死亡。2. Disposable straw: This type of straw is not easy to hold, it is difficult to control when blowing cells, and it is easy to damage the cell layer; and the suction head is relatively short, which may easily cause the culture medium with cells to remain in the straw when blowing cells In the head, the increased cells are damaged or even die due to excessive extrusion.

3.移液器：目前实验中多采用此设备进行吹打细胞，使用过程中也存在上述问题，即要控制吹打的力度又要控制枪头不要直接摩擦瓶壁导致细胞直接死亡；若传代细胞过多容易造成按压移液器的手指疲劳，增大细胞机械性损伤的数量，而且人为的控制吹打力度，也会造成吹打力度不一致，可能出现一会力度大，一会力度小，容易即出现细胞吹打不充分，细胞抱团，又出现因力度过大导致细胞损伤的情况。3. Pipette: At present, this equipment is often used to pipette cells in experiments. The above-mentioned problems also exist in the process of use. It is necessary to control the force of pipetting and control the tip of the pipette so that the cells do not directly rub against the wall of the bottle and cause the cells to die directly; It is easy to cause the fatigue of the fingers pressing the pipette and increase the number of mechanical damage to the cells. Moreover, the artificial control of the blowing force will also cause inconsistent blowing force. It may appear that the force is high for a while, and the force is low for a while, and cells are prone to appear. Insufficient pipetting, cells clumped together, and cell damage occurred due to excessive force.

综上所述，在细胞传代培养过程中，使用现有的工具操作者无法稳定的控制吹打力度，效率低，劳动强度大，容易导致细胞损伤，影响实验。To sum up, in the process of cell subculture, the operator cannot stably control the force of pipetting with existing tools, which is inefficient and labor-intensive, which easily leads to cell damage and affects the experiment.

实用新型内容Utility model content

为了解决上述现有技术存在的不足，本实用新型提供了一种传代培养自动吹打器，能够恒定力度的自动吹打细胞，提高传动操作的稳定性，使操作者更加省力，防止细胞损伤。In order to solve the deficiencies in the above-mentioned prior art, the utility model provides an automatic pipetting device for subculture, which can automatically blow cells with a constant force, improve the stability of the transmission operation, save the operator more effort, and prevent cell damage.

本实用新型解决其技术问题所采用的技术方案是：一种传代培养自动吹打器，包括U型卡扣、握柄、开关、支架、缓冲装置、调速器旋钮和伸缩装置， U型卡扣扣盖在移液器侧壁上，U型卡扣端部设有挡环，U型卡扣侧壁固定连接有握柄，U型卡扣后端通过支架连接伸缩装置，伸缩装置的伸缩杆端与移液器的按钮在同一轴线上，伸缩装置的伸缩杆端设有顶压移液器按钮的缓冲装置，握柄内设有电池和伸缩装置的驱动器，伸缩装置的驱动器上设有调速旋钮，握柄上设有分别于电池和伸缩装置的驱动器连接的开关。The technical scheme adopted by the utility model to solve the technical problems is: an automatic blower for subculture, including a U-shaped buckle, a handle, a switch, a bracket, a buffer device, a governor knob and a telescopic device, and the U-shaped buckle The buckle cover is on the side wall of the pipette. There is a stop ring at the end of the U-shaped buckle. The side wall of the U-shaped buckle is fixedly connected with a handle. The rear end of the U-shaped buckle is connected to the telescopic device through the bracket. The end of the telescopic device is on the same axis as the button of the pipette. The end of the telescopic rod of the telescopic device is provided with a buffer device that presses the button of the pipette. The battery and the driver of the telescopic device are installed in the handle. Speed knob, handle is provided with the switch that is respectively connected with the driver of battery and retractable device.

进一步的，所述的伸缩装置为齿轮齿条机构或伸缩旋转减速电机。Further, the telescopic device is a rack and pinion mechanism or a telescopic rotary geared motor.

进一步的，所述的缓冲装置为O型钢环，O型钢环侧壁与伸缩装置的伸缩杆端固定连接，O型钢环具有一定韧性，其形变力大于移液器的按钮弹簧的弹力。Further, the buffer device is an O-shaped steel ring, the side wall of the O-shaped steel ring is fixedly connected with the end of the telescopic rod of the telescopic device, the O-shaped steel ring has a certain toughness, and its deformation force is greater than the elastic force of the button spring of the pipette.

本实用新型的有益效果是：The beneficial effects of the utility model are:

1、提供了一种能够装卡移液器的自动吹打装置，使移液器能够保持恒定力度的循环吹打，提高吹打效果，吹打速度可调，吹打量可通过调节移液器实现调节，避免剪切力过大，损伤细胞；更方便握持，便于控制与瓶壁的距离，避免细胞被研磨。1. Provides an automatic pipetting device that can hold a pipette, so that the pipette can maintain a constant force of circular pipetting, improve the blowing effect, the blowing speed is adjustable, and the blowing volume can be adjusted by adjusting the pipette to avoid Excessive shear force will damage the cells; it is more convenient to hold, and it is easy to control the distance from the bottle wall to avoid the cells from being grinded.

2、结构简单，只需一步操作，便可实现移液器的拆装，全程不与培养瓶、培养皿接触，更加卫生安全，体积小巧、轻便，容易控制的同时，设有内置锂电池的握把，在便携的同时，也方便了操作者的操控。2. The structure is simple, and the pipette can be disassembled and assembled with only one step of operation. It does not touch the culture bottle or culture dish during the whole process, which is more hygienic and safe. It is small, light, easy to control, and has a built-in lithium battery. The handle, while being portable, is also convenient for the operator to control.

附图说明Description of drawings

下面结合附图和具体实施方式对本实用新型进一步说明。Below in conjunction with accompanying drawing and specific embodiment the utility model is further described.

图1为本实用新型实施例1的结构示意图。Fig. 1 is a schematic structural view ofEmbodiment 1 of the present utility model.

图2为本实用新型实施例1的结构示意图。Fig. 2 is a schematic structural view ofEmbodiment 1 of the present utility model.

图3为本实用新型实施例1安装移液器后的结构示意图。Fig. 3 is a structural schematic diagram of theutility model embodiment 1 after installing a pipette.

图4为本实用新型实施例2的结构示意图。Fig. 4 is a schematic structural diagram ofEmbodiment 2 of the present utility model.

图中标号：Labels in the figure:

1、U型卡扣，2、握柄，3、开关，4、支架，5、齿轮齿条机构，6、缓冲装置，7、调速旋钮，8、伸缩旋转减速电机，9、移液器，51、滑套，52、数字舵机，53、齿条，54、齿轮。1. U-shaped buckle, 2. Handle, 3. Switch, 4. Bracket, 5. Rack and pinion mechanism, 6. Buffer device, 7. Speed control knob, 8. Telescopic rotary gear motor, 9. Pipette , 51, sliding sleeve, 52, digital steering gear, 53, rack, 54, gear.

具体实施方式Detailed ways

实施例1Example 1

如图1-3所示，一种传代培养自动吹打器，包括U型卡扣1、握柄2、开关3、支架4、缓冲装置6、调速器旋钮7和伸缩装置， U型卡扣1扣盖在移液器9侧壁上，其内径形状与移液器9的外径形状相同，开口端部略向内收缩，便于抱紧移液器9，U型卡扣1端部设有等比例内缩的挡环，从而防止卡入内部的移液器9向前顶出。As shown in Figure 1-3, an automatic blower for subculture includes aU-shaped buckle 1, ahandle 2, aswitch 3, abracket 4, abuffer device 6, agovernor knob 7 and a telescopic device, and theU-shaped buckle 1. The button cover is on the side wall of thepipette 9. Its inner diameter and shape are the same as the outer diameter of thepipette 9. The end of the opening is slightly shrunk inwards, so that it is easy to hold thepipette 9 tightly. The end of theU-shaped buckle 1 is set There is a retaining ring retracted in equal proportion, so as to prevent thepipette 9 stuck inside from being ejected forward.

U型卡扣1侧壁固定连接有握柄2，缩短握持点与移液器9枪杆根部的距离，便于操控也便于握持。The side wall of theU-shaped buckle 1 is fixedly connected with thehandle 2, which shortens the distance between the holding point and the root of thepipette 9 gun shaft, and is convenient for manipulation and grip.

U型卡扣1后端通过支架4连接伸缩装置，伸缩装置为齿轮齿条机构5，包括固定板、滑套51、数字舵机52、齿条53和齿轮54，支架4尾端设有固定板，固定板与滑套51侧壁固定连接，滑套51内设有能够前后滑动的齿条53，齿条53为伸缩装置的伸缩杆，齿条53的中心线与移液器9的按钮轴线在同一直线内，固定板背面设有数字舵机52，数字舵机52的轴端从固定板端面伸出，伸出端数设有与齿条53啮合的齿轮54，数字舵机52往复运转，齿条53实现往复伸缩。The rear end of theU-shaped buckle 1 is connected to the telescopic device through thebracket 4, and the telescopic device is a rack andpinion mechanism 5, including a fixed plate, a slidingsleeve 51, adigital steering gear 52, arack 53 and agear 54, and the rear end of thebracket 4 is provided with a fixed plate, the fixed plate is fixedly connected with the side wall of the slidingsleeve 51, and the slidingsleeve 51 is provided with arack 53 that can slide forward and backward. The axes are in the same straight line, the back of the fixed plate is provided with adigital steering gear 52, the shaft end of thedigital steering gear 52 protrudes from the end face of the fixed plate, and the number of the protruding end is provided with agear 54 meshing with therack 53, and thedigital steering gear 52 reciprocates , Therack 53 realizes reciprocating expansion and contraction.

齿条53端部设有顶压移液器9按钮的缓冲装置6，由于移液器9本身自带移液量的调节，可利于移液器9本身的移液量调节实现吹打量的控制，但调节完成后，移液器9按钮的压缩行程会发生变化，即移液量越少，压缩行程越短，调节的方式是通过旋转移液器9的旋钮实现的，为避免行程变短后，伸缩杆与移液器9发生硬冲击，特别增设缓冲装置，缓冲装置6为O型钢环，O型钢环侧壁与伸缩装置的伸缩杆端固定连接，O型钢环具有一定韧性，其形变力大于移液器9的按钮弹簧的弹力，当伸缩杆向前伸出时，由于O型钢环的形变力大于移液器9的按钮弹簧的弹力，从而实现对移液器9的按钮弹簧的压缩，而当移液器9缩小移液量后，压缩形成变短，伸缩杆伸缩距离不变的情况下，O型钢环被压缩，缓冲伸缩杆伸出的多余部分，使机器稳定运行。The end of therack 53 is provided with abuffer device 6 that presses the button of thepipette 9. Since thepipette 9 itself has its own adjustment of the pipetting volume, it can facilitate the adjustment of the pipetting volume of thepipette 9 itself to realize the control of the blowing volume. , but after the adjustment is completed, the compression stroke of thepipette 9 button will change, that is, the less the pipetting volume, the shorter the compression stroke. The adjustment method is realized by rotating the knob of thepipette 9. Finally, the telescopic rod and thepipette 9 have a hard impact, and a buffer device is specially added. Thebuffer device 6 is an O-shaped steel ring, and the side wall of the O-shaped steel ring is fixedly connected with the end of the telescopic rod of the telescopic device. The force is greater than the elastic force of the button spring of thepipette 9. When the telescopic rod stretched forward, the deformation force of the O-shaped steel ring was greater than the elastic force of the button spring of thepipette 9, thereby realizing the elastic force of the button spring of thepipette 9. Compression, and when thepipette 9 reduces the pipetting volume, the compression becomes shorter, and the stretching distance of the telescopic rod remains the same, the O-shaped steel ring is compressed, buffering the excess part of the telescopic rod, so that the machine runs stably.

握柄1内设有电池和伸缩装置驱动器，伸缩装置的驱动器为舵机驱动器，数字舵机52与舵机驱动器连接，握柄1上设有分别于电池和伸缩装置的驱动器连接的开关3，电池上设有充放电保护模块和充电接头，可直接通过电源适配器进行充电。Thehandle 1 is provided with a battery and a telescopic device driver, and the driver of the telescopic device is a steering gear driver. Thedigital steering gear 52 is connected to the steering gear driver. Thehandle 1 is provided with aswitch 3 connected to the battery and the telescopic device driver respectively. The battery is equipped with a charge and discharge protection module and a charging connector, which can be charged directly through a power adapter.

实施例2Example 2

如图4所示，一种传代培养自动吹打器，包括U型卡扣1、握柄2、开关3、支架4、缓冲装置6、调速器旋钮7和伸缩装置， U型卡扣1扣盖在移液器9侧壁上，其内径形状与移液器9的外径形状相同，开口端部略向内收缩，便于抱紧移液器9，U型卡扣1端部设有等比例内缩的挡环，从而防止卡入内部的移液器9向前顶出。As shown in Figure 4, an automatic blower for subculture includes aU-shaped buckle 1, ahandle 2, aswitch 3, abracket 4, abuffer device 6, agovernor knob 7 and a telescopic device, and aU-shaped buckle 1 buckle Covered on the side wall of thepipette 9, its inner diameter shape is the same as the outer diameter shape of thepipette 9, and the opening end is slightly shrunk inward, which is convenient for holding thepipette 9 tightly. The baffle ring shrinks in proportion, thereby preventing thepipette 9 stuck inside from being ejected forward.

U型卡扣1侧壁固定连接有握柄2，缩短握持点与移液器9枪杆根部的距离，便于操控也便于握持。The side wall of theU-shaped buckle 1 is fixedly connected with thehandle 2, which shortens the distance between the holding point and the root of thepipette 9 gun shaft, and is convenient for manipulation and grip.

U型卡扣1后端通过支架4连接伸缩装置，伸缩装置为伸缩旋转减速电机8，伸缩旋转减速电机8包括电机、减速器、齿轮杆、大齿轮、滑杆、滑板、往复螺杆、端板、套筒、顶丝和延伸杆，电机轴端设有减速器，减速器通过小齿轮与齿轮杆啮合，齿轮杆两侧设有固定在减速器端部的滑杆，滑杆端部设有端板，端板与减速器间设有套有滑杆上的滑板，滑板能够在滑杆上平移，滑板上开设有齿轮杆穿过的过孔，滑板中心设有能够转动的往复螺杆，往复螺杆底端设有与齿轮杆啮合的大齿轮，端板中心设有套在往复螺杆上的套筒，套筒侧壁设有顶入往复螺杆螺纹内的顶丝，往复螺杆端部设有从套筒端部伸出的延伸杆，延伸杆为伸缩装置的伸缩杆，伸缩装置的驱动器为电机调速器，电机调速器与电机连接，电机运转，通过减速器驱动齿轮杆转动，齿轮杆带动带齿轮运转，从而使同轴的往复螺杆转动，在顶丝的限位下，往复螺杆在套筒内实现伸缩或收缩运动，伸缩和收缩的过程中，滑板同往复螺杆一同在滑杆上移动，滑板移动过程中，大齿轮始终与齿轮杆啮合，保持稳定的工作。The rear end of theU-shaped buckle 1 is connected to the telescopic device through thebracket 4. The telescopic device is a telescopicrotary deceleration motor 8. The telescopicrotary deceleration motor 8 includes a motor, a reducer, a gear rod, a large gear, a slide rod, a slide plate, a reciprocating screw, and an end plate. , sleeve, top wire and extension rod, the motor shaft end is equipped with a reducer, the reducer meshes with the gear rod through the pinion, the two sides of the gear rod are provided with sliding rods fixed at the end of the reducer, and the end of the sliding rod is provided with End plate, between the end plate and the reducer, there is a slide plate covered with a slide bar. The slide plate can move in translation on the slide bar. There is a via hole through which the gear rod passes on the slide plate. There is a reciprocating screw that can rotate in the center of the slide plate. The bottom end of the screw is provided with a large gear meshing with the gear rod, the center of the end plate is provided with a sleeve on the reciprocating screw, the side wall of the sleeve is provided with a jacking screw that is pushed into the thread of the reciprocating screw, and the end of the reciprocating screw is provided with a The extension rod protruding from the end of the sleeve, the extension rod is the telescopic rod of the telescopic device, the driver of the telescopic device is a motor governor, the motor governor is connected with the motor, the motor runs, and the gear rod is driven to rotate through the reducer, and the gear rod Drive the belt gear to run, so that the coaxial reciprocating screw rotates. Under the limit of the top screw, the reciprocating screw realizes telescopic or shrinking movement in the sleeve. During the movement of the skateboard, the large gear is always engaged with the gear rod to maintain stable work.

延伸杆端部设有顶压移液器9按钮的缓冲装置6，由于移液器9本身自带移液量的调节，可利于移液器9本身的移液量调节实现吹打量的控制，但调节完成后，移液器9按钮的压缩行程会发生变化，即移液量越少，压缩行程越短，调节的方式是通过旋转移液器9的旋钮实现的，为避免行程变短后，延伸杆与移液器9发生硬冲击，特别增设缓冲装置，缓冲装置6为O型钢环，O型钢环侧壁与伸缩装置的伸缩杆端固定连接，O型钢环具有一定韧性，其形变力大于移液器9的按钮弹簧的弹力，当伸缩杆向前伸出时，由于O型钢环的形变力大于移液器9的按钮弹簧的弹力，从而实现对移液器9的按钮弹簧的压缩，而当移液器9缩小移液量后，压缩形成变短，伸缩杆伸缩距离不变的情况下，O型钢环被压缩，缓冲伸缩杆伸出的多余部分，使机器稳定运行。The end of the extension rod is provided with abuffer device 6 that presses the button of thepipette 9. Since thepipette 9 itself has its own adjustment of the pipetting volume, it can facilitate the adjustment of the pipetting volume of thepipette 9 itself to realize the control of the blowing volume. But after the adjustment is completed, the compression stroke of thepipette 9 button will change, that is, the less the pipetting volume, the shorter the compression stroke. The adjustment method is realized by rotating the knob of thepipette 9. , the extension rod and thepipette 9 have a hard impact, and a buffer device is specially added. Thebuffer device 6 is an O-shaped steel ring, and the side wall of the O-shaped steel ring is fixedly connected with the end of the telescopic rod of the telescopic device. Greater than the elastic force of the button spring of thepipette 9, when the telescopic rod stretches forward, since the deformation force of the O-shaped steel ring is greater than the elastic force of the button spring of thepipette 9, the compression of the button spring of thepipette 9 is realized , and when thepipette 9 reduces the pipetting volume, the compressed form becomes shorter, and the stretching distance of the telescopic rod remains unchanged, the O-shaped steel ring is compressed to buffer the excess part of the telescopic rod, so that the machine runs stably.

握柄1内设有电池和伸缩装置驱动器，伸缩装置的驱动器为舵机驱动器，数字舵机52与舵机驱动器连接，握柄1上设有分别于电池和伸缩装置的驱动器连接的开关3，电池上设有充放电保护模块和充电接头，可直接通过电源适配器进行充电。Thehandle 1 is provided with a battery and a telescopic device driver, and the driver of the telescopic device is a steering gear driver. Thedigital steering gear 52 is connected to the steering gear driver. Thehandle 1 is provided with aswitch 3 connected to the battery and the telescopic device driver respectively. The battery is equipped with a charge and discharge protection module and a charging connector, which can be charged directly through a power adapter.

该装置利于伸缩装置解决了在细胞传代培养过程中无法稳定的控制吹打力度的问题，使移液器能够保持恒定力度的循环吹打，吹打行程一致，提高吹打效果，吹打速度可调，吹打量可通过调节移液器实现调节，避免剪切力过大，损伤细胞；更方便握持，便于控制与瓶壁的距离，避免细胞被研磨。The device is beneficial to the telescopic device to solve the problem that the blowing force cannot be stably controlled during the cell subculture process, so that the pipette can maintain a constant force of circular blowing, the blowing stroke is consistent, the blowing effect is improved, the blowing speed is adjustable, and the blowing volume can be adjusted. Adjustment is achieved by adjusting the pipette to avoid excessive shear force and cell damage; it is more convenient to hold and control the distance from the bottle wall to avoid cells being ground.

Claims (4)

Translated fromChinese

1.一种传代培养自动吹打器，其特征在于：包括U型卡扣（1）、握柄（2）、开关（3）、支架（4）、缓冲装置（6）、调速器旋钮（7）和伸缩装置， U型卡扣（1）扣盖在移液器（9）侧壁上，U型卡扣（1）端部设有挡环，U型卡扣（1）侧壁固定连接有握柄（2），U型卡扣（1）后端通过支架（4）连接伸缩装置，伸缩装置的伸缩杆端与移液器（9）的按钮在同一轴线上，伸缩装置的伸缩杆端设有顶压移液器（9）按钮的缓冲装置（6），握柄（2）内设有电池和伸缩装置的驱动器，伸缩装置的驱动器上设有调速旋钮（7），握柄（2）上设有分别于电池和伸缩装置的驱动器连接的开关（3）。1. An automatic blower for subculture, characterized in that it includes a U-shaped buckle (1), a handle (2), a switch (3), a bracket (4), a buffer device (6), and a governor knob ( 7) and the retractable device, the U-shaped buckle (1) is buckled on the side wall of the pipette (9), the end of the U-shaped buckle (1) is provided with a retaining ring, and the U-shaped buckle (1) is fixed on the side wall The handle (2) is connected, and the rear end of the U-shaped buckle (1) is connected to the telescopic device through the bracket (4). The telescopic rod end of the telescopic device is on the same axis as the button of the pipette (9). The end of the rod is provided with a buffer device (6) for pressing the button of the pipette (9), and the handle (2) is provided with a battery and a driver of the telescopic device, and a speed regulating knob (7) is provided on the driver of the telescopic device. The handle (2) is provided with a switch (3) connected to the battery and the driver of the telescopic device respectively.2.根据权利要求1所述的一种传代培养自动吹打器，其特征在于：伸缩装置为齿轮齿条机构（5），包括固定板、滑套（51）、数字舵机（52）、齿条（53）和齿轮（54），支架（4）尾端设有固定板，固定板与滑套（51）侧壁固定连接，滑套（51）内设有齿条（53），齿条（53）为伸缩装置的伸缩杆，齿条（53）的中心线与移液器（9）的按钮轴线在同一直线内，固定板背面设有数字舵机（52），数字舵机（52）的轴端设有与齿条（53）啮合的齿轮（54），伸缩装置驱动器为舵机驱动器，数字舵机（52）与舵机驱动器连接。2. An automatic blower for subculture according to claim 1, characterized in that: the telescopic device is a rack and pinion mechanism (5), including a fixed plate, a sliding sleeve (51), a digital steering gear (52), a gear bar (53) and gear (54), the tail end of the bracket (4) is provided with a fixed plate, the fixed plate is fixedly connected with the side wall of the sliding sleeve (51), the sliding sleeve (51) is provided with a rack (53), and the rack (53) is the telescopic rod of the telescopic device, the center line of the rack (53) is in the same line as the axis of the button of the pipette (9), the back of the fixed plate is provided with a digital steering gear (52), and the digital steering gear (52 ) is provided with a gear (54) engaged with the rack (53), the telescoping device driver is a steering gear driver, and the digital steering gear (52) is connected to the steering gear driver.3.根据权利要求1所述的一种传代培养自动吹打器，其特征在于：缓冲装置（6）为O型钢环，O型钢环侧壁与伸缩装置的伸缩杆端固定连接，O型钢环具有一定韧性，其形变力大于移液器（9）的按钮弹簧的弹力。3. An automatic blower for subculture according to claim 1, characterized in that: the buffer device (6) is an O-shaped steel ring, the side wall of the O-shaped steel ring is fixedly connected with the telescopic rod end of the telescopic device, and the O-shaped steel ring has Certain toughness, its deformation force is greater than the elastic force of the button spring of pipette (9).4.根据权利要求1所述的一种传代培养自动吹打器，其特征在于：伸缩装置为伸缩旋转减速电机（8），伸缩装置的驱动器为电机调速器，电机调速器与电机连接。4. An automatic pipetting device for subculture according to claim 1, characterized in that the telescopic device is a telescopic rotary geared motor (8), the driver of the telescopic device is a motor governor, and the motor governor is connected to the motor.

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