CN214471835U - A frozen rat brain slice device - Google Patents

Abstract

Translated fromChinese

本实用新型提供了一种冰冻鼠脑切片装置，包括：金属底座，设置在底座上的抽屉，固定在底座顶部的第一箱体和第二箱体，抽屉、第一箱体和第二箱体用于存放冰块，能够使该装置处于低温状态，避免鼠脑发生解冻，影响切片效果；同时，第一箱体和第二箱体之间设置有刀架机构，刀架机构滑动连接在第一箱体和第二箱体上，能够对切片厚度进行调节，还能避免刀刃偏移，保证切片质量；底座上还设置有载物台，第一箱体和第二箱体位于载物台的两侧，载物台的另外两侧分别设置有第一调整机构和第一挡板，第一调整机构和第一挡板用于夹持鼠脑，能够固定住鼠脑，避免鼠脑产生移动，影响切片效果。

The utility model provides a frozen rat brain slice device, comprising: a metal base, a drawer arranged on the base, a first box body and a second box body fixed on the top of the base, the drawer, the first box body and the second box body The body is used to store ice cubes, which can keep the device in a low temperature state to avoid thawing of the mouse brain and affect the slicing effect; at the same time, a knife holder mechanism is arranged between the first box body and the second box body, and the knife holder mechanism is slidably connected to the On the first box body and the second box body, the thickness of the slice can be adjusted, and the offset of the knife edge can be avoided to ensure the quality of the slice; The two sides of the stage and the other two sides of the stage are respectively provided with a first adjustment mechanism and a first baffle. The first adjustment mechanism and the first baffle are used to clamp the rat brain, which can fix the rat brain and avoid the rat brain. Creates movement that affects the slicing effect.

Description

Frozen rat brain section device

Technical Field

The application relates to the technical field of animal experiment instruments, in particular to a frozen rat brain section device.

Background

The rat has the characteristics of moderate size, fast reproduction, easy feeding, mild temperament, convenient administration, diversified behaviors and the like, so the rat is widely applied to medical research. Neuroscience experiments often require rat brain tissue to be frozen and then sliced to obtain target nuclei.

At present, the slicing method is that the rat brain is placed in a glass slide or a slicing groove for manual slicing, on one hand, due to the fact that the number of slices is large, the rat brain cannot be guaranteed to be always in a frozen state, and the situation that the rat brain is unfrozen after a certain time possibly occurs, and the slicing effect is affected. In the second aspect, because the rat brain is in a frozen state, when the blade cuts down, one end of the blade which is not cut is easy to tilt, so that the rat brain moves, the cutting trace of the blade can also deviate, the slicing effect is influenced, and the experimental result is further influenced. In the third aspect, because the sizes of the existing slicing grooves are fixed, the fixing effect is not good for the rat brains with different sizes, and the slicing grooves with various specifications are required to be prepared, so that the experiment cost is higher.

SUMMERY OF THE UTILITY MODEL

In order to solve the defects in the prior art, the inventor provides a frozen rat brain slicing device which can ensure that the rat brain is sliced at a low temperature and has a good fixing effect, and the device is suitable for rat brains of various sizes.

Specifically, the utility model discloses a realize like this:

a frozen rat brain section apparatus, the apparatus comprising: the ice cube storage box comprises a base made of metal, a drawer arranged on the base, a first box body and a second box body arranged at the top of the base, wherein the drawer, the first box body and the second box body are used for storing ice cubes; a tool rest mechanism is arranged between the first box body and the second box body and is connected to the first box body and the second box body in a sliding manner;

the mouse brain clamping device is characterized in that an object stage is further arranged on the base, the first box body and the second box body are located on two sides of the object stage, a first adjusting mechanism and a first baffle are respectively arranged on the other two sides of the object stage, and the first adjusting mechanism and the first baffle are used for clamping mouse brains.

Furthermore, a second adjusting mechanism is further arranged on the base, and the second box body is connected to the second adjusting mechanism in a sliding mode.

Furthermore, a first slide rail is arranged on the first box body, and a second slide rail is arranged on the second box body; the tool rest mechanism is connected to the first slide rail and the second slide rail in a sliding manner;

the tool rest mechanism comprises: the first upright post is connected to the first slide rail in a sliding manner and is detachably connected with the first slide rail, and the second upright post is connected to the second slide rail in a sliding manner and is detachably connected with the second slide rail; a first vertical rod is slidably arranged in the first upright column, a second vertical rod is slidably arranged in the second upright column, springs are sleeved on the first vertical rod and the second vertical rod, the tops of the first vertical rod and the second vertical rod are connected with the cross rod, and the bottoms of the first vertical rod and the second vertical rod are connected with blade mounting seats; the blade mounting seat is provided with a blade, the lower parts of the first upright post and the second upright post are both provided with a blade groove, and the blade is arranged in the blade groove.

Furthermore, a first slide block is arranged on the first upright post, the first slide block is connected to the first slide rail in a sliding manner, a second slide block is arranged on the second upright post, and the second slide block is connected to the second slide rail in a sliding manner; the first sliding block is provided with a first nut, the first nut is used for fixing the first sliding block on the first sliding rail, the second sliding block is provided with a second nut, and the second nut is used for fixing the second sliding block on the second sliding rail.

Furthermore, one end of the cross rod, which is close to the second upright post, is provided with a sliding groove, the top of the second upright post is fixedly provided with a sliding rod, and the sliding rod is connected in the sliding groove in a sliding manner.

Further, be provided with the scale on first slide rail and the second slide rail, all be provided with the recess on first stand and the second stand, the recess aligns with the blade.

Further, the first adjustment mechanism includes: fix fixed block on the base passes the first screw rod of fixed block, sliding connection be in second baffle on the base, when slicing into slices, the one end that first screw rod is close to the objective table withstands the second baffle.

Further, the second adjustment mechanism includes: the second screw rod penetrates through the third baffle and is connected with the third slide rail, and the second box body is connected to the third slide rail in a slidable mode.

The utility model discloses a theory of operation introduces:

firstly, put the ice-cube in drawer, first box and second box, guarantee that the device is in the low temperature state, then put the sliced mouse brain of needs on the objective table, grasp the mouse brain through first guiding mechanism and first baffle, rethread second guiding mechanism makes first box and second box withstand the other two sides of mouse brain, fixes the mouse brain from four directions, transmits the mouse brain with low temperature simultaneously on, avoids the mouse brain to unfreeze. And then selecting a blade with a proper size for installation, sliding a blade carrier mechanism into the blade carrier mechanism through one end of the first sliding rail and one end of the second sliding rail after installation, suspending the blade above the mouse brain, adjusting the thickness of the slice according to the scale according to the requirement, fixing the slice by using the first nut and the second nut after adjustment, preventing the blade from moving, and then pressing the cross rod to enable the blade to sink for slicing.

Compared with the prior art, the beneficial effects of the utility model introduce:

(1) the ice blocks are placed in the drawer, the first box body and the second box body, and meanwhile, the first box body and the second box body are in direct contact with the mouse brain, so that the mouse brain can be guaranteed to be in a frozen state, and the slicing quality is guaranteed.

(2) Through setting up first guiding mechanism and second guiding mechanism for the device can guarantee to the fixed of mouse brain, avoids the mouse brain to take place to remove, guarantees the section quality, can adapt to the mouse brain of different size sizes simultaneously, improves the adaptability.

(3) The knife rest mechanism is connected to the first box and the second box in a sliding mode, so that slicing thickness can be adjusted conveniently, meanwhile, the knife rest mechanism can also be fixed to the first box and the second box, cutting edge deviation can be avoided, and slicing effect is guaranteed.

Drawings

FIG. 1 is a diagram showing the state of use of the frozen rat brain section apparatus in example 1;

FIG. 2 is a schematic structural view of a frozen rat brain section apparatus in example 1;

FIG. 3 is an exploded view of the frozen rat brain slice apparatus in example 1;

FIG. 4 is a schematic structural view of a blade holder mechanism inembodiment 1;

FIG. 5 is an exploded view of the blade holder mechanism inembodiment 1;

FIG. 6 is an enlarged view of a portion of FIG. 1 at A;

reference numerals:

1-a base; 11-an object stage; 12-a first baffle; 2-a drawer; 3-a first box body; 31-a first slide rail; 4-a second box body; 41-a second slide rail; 5-a tool holder mechanism; 51-a first upright; 511-a first upright; 512-a first slider; 513 — a first nut; 52-a second upright; 521-a second upright stanchion; 522-a second slider; 523-second nut; 53-a cross-bar; 531-chute; 54-a spring; 55-a blade mount; 56-a knife slot; 57-blade; 58-a slide bar; 59-grooves; 6-a first adjustment mechanism; 61-a fixed block; 62-a first screw; 63-a second baffle; 7-a second adjustment mechanism; 71-a third slide rail; 72-a second screw; 73-a third baffle; 8-graduation.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

Example 1

As shown in fig. 1-3, the utility model provides a frozen rat brain section device, the device includes: the ice cube storage box comprises abase 1 made of metal, adrawer 2 arranged on thebase 1, afirst box body 3 fixed to the top of thebase 1 and asecond box body 4 connected to the top of thebase 1 in a sliding mode, wherein thedrawer 2, thefirst box body 3 and thesecond box body 4 are used for storing ice cubes. The device is cooled through the ice blocks, so that the device is always kept in a low-temperature state, and the unfreezing of frozen rat brain is avoided, and the slicing effect is not influenced. Be provided with knife rest mechanism betweenfirst box 3 and thesecond box 4, be provided withfirst slide rail 31 on thefirst box 3, be provided withsecond slide rail 41 on thesecond box 4, knife rest mechanism sliding connection is onfirst slide rail 31 andsecond slide rail 41, the both ends offirst slide rail 31 andsecond slide rail 41 do not stop for knife rest mechanism can be followedfirst slide rail 31 andsecond slide rail 41 and slided down, realizes dismantling of knife rest mechanism, the installation and the change of the cutter of being convenient for. Thebase 1 is further provided with an object stage 11, thefirst box 3 and thesecond box 4 are located on two sides of the object stage 11, the other two sides of the object stage 11 are respectively provided with a first adjusting mechanism and afirst baffle 12, and the first adjusting mechanism and thefirst baffle 12 are used for clamping the rat brain. Specifically, the first adjusting mechanism can be adjusted according to the size of the mouse brain, so that the mouse brain is clamped by the first adjusting mechanism and thefirst baffle 12; simultaneously, becausesecond box 4 is sliding connection withbase 1, usablefirst box 3 andsecond box 4 carry out the centre gripping to the rat brain, also be convenient for low temperature conduction simultaneously to the rat brain, avoid the rat brain to unfreeze. Still be provided with second guidingmechanism 7 on thebase 1,second box 4 sliding connection is on second guidingmechanism 7, and second guidingmechanism 7 is used for fixingsecond box 4, avoids the section in-process to take place to remove, influences the section effect. It should be understood that the size of the object stage 11 should be smaller than the size of the rat brain, so as to avoid the object stage 11 blocking the first adjusting mechanism, thefirst baffle 12, thefirst box 3 and thesecond box 4 to clamp the rat brain.

Specifically, the first adjustment mechanism includes: thefixed block 61 fixed on thebase 1, thefirst screw 62 penetrating through thefixed block 61, and thesecond baffle 63 movably connected on thebase 1, when slicing, thesecond baffle 63 is pushed by one end of thefirst screw 62 close to the objective table 11. Thefirst screw 62 can be adjusted by adjusting the distance between thefirst baffle 12 and thesecond baffle 63, and the mouse brain clamping device is suitable for clamping mouse brains with different sizes. In addition, thesecond baffle 63 can be taken down after slicing, so that the rat brain slice adhered to thesecond baffle 63 can be taken down conveniently. Thesecond adjustment mechanism 7 includes: thethird slide rail 71, thesecond screw 72 disposed in thethird slide rail 71, and thethird baffle 73 disposed at one end of thethird slide rail 71 away from thefirst box 3, thesecond screw 72 passes through thethird baffle 73 to be connected to thethird slide rail 71, and thesecond box 4 is slidably connected to the third slide rail 71. Make the interval offirst box 3 andsecond box 4 adjustable, can the not unidimensional rat brain of centre gripping, simultaneously, can also restrictsecond box 4 when the section, avoid it to take place to remove.

Further, as shown in fig. 4 to 6, theblade carrier mechanism 5 includes: the vertical column comprises a firstvertical column 51, a secondvertical column 52 and across rod 53, wherein a firstvertical rod 511 is arranged in the firstvertical column 51, the firstvertical rod 511 can slide up and down in the firstvertical column 51, a secondvertical rod 521 is arranged in the secondvertical column 52, the secondvertical rod 521 can slide up and down in the secondvertical column 52, andsprings 54 are sleeved on the firstvertical rod 511 and the secondvertical rod 521. The bottom parts of the firstvertical rod 511 and the secondvertical rod 521 are both connected with ablade mounting seat 55, ablade 57 is mounted on theblade mounting seat 55, the lower parts of the firstvertical rod 51 and the secondvertical rod 52 are both provided with aknife groove 56, and theblade 57 is arranged in theknife groove 56. Through pressinghorizontal pole 53, makefirst pole setting 511 andsecond pole setting 521 lapse downwards, useblade 57 to slice, simultaneously, can compressspring 54, slice the completion back, under the spring action ofspring 54, makehorizontal pole 53 rise, the reconversion effectively improves section efficiency. One end of thecross rod 53 is fixedly connected with the firstupright post 51, the other end of the cross rod is provided with asliding groove 531, the top of the secondupright post 521 is fixedly provided with asliding rod 58, thesliding rod 58 is slidably connected in thesliding groove 531, the distance between the firstupright post 51 and the secondupright post 52 can be adjusted by moving thesliding rod 58, the size of theblade 57 can be adjusted, the slicing device can adapt to brains of mice with different sizes, and the applicability of the slicing device is improved. Be provided withfirst slider 512 on thefirst stand 51,first slider 512 sliding connection is onfirst slide rail 31 to, be provided withfirst nut 513 on thefirst slider 512,first nut 513 is used for fixingfirst slider 512 onfirst slide rail 31 when the section, can avoidblade 57 to take place the skew, influences the section effect. Similarly, thesecond column 52 is provided with asecond slider 522, thesecond slider 522 is slidably connected to thesecond slide rail 41, thesecond slider 522 is provided with asecond nut 523, and thesecond nut 523 is used for fixing thesecond slider 522 on thesecond slide rail 41 during slicing. Further, be provided withscale 8 onfirst slide rail 31 and thesecond slide rail 41, all be provided withrecess 59 onfirst stand 51 and thesecond stand 52,recess 59 aligns withblade 57, and during the section,accessible recess 59 makesblade 57align scale 8, guarantees the accurate nature of section thickness, improves the section quality.

Specifically, the utility model discloses a work flow as follows:

firstly, loosening thefirst nut 513 and thesecond nut 523 to enable thetool rest mechanism 5 to slide out of thefirst slide rail 31 and thesecond slide rail 41, then placing ice blocks in thedrawer 2, thefirst box 3 and thesecond box 4, then placing the mouse brain on the object stage 11, clamping the mouse brain from four directions by rotating thefirst screw rod 62 and thesecond screw rod 72 to enable thefirst baffle plate 12, thesecond baffle plate 63, thefirst box 3 and thesecond box 4, then selecting ablade 57 with proper size, adjusting the distance between the firstupright post 51 and the secondupright post 52 through theslide rod 58, after the adjustment is completed, installing theblade 57 on theblade installation seat 55, then installing thetool rest mechanism 5 on thefirst slide rail 3 and thesecond slide rail 41, then aligning thegroove 59 with thescale 8 according to the slice thickness, screwing thefirst nut 513 and thesecond nut 523, driving theblade 57 to sink by pressing thecross rod 53, slicing is performed.

The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.

Claims (8)

1. A frozen rat brain section device is characterized by comprising: the ice cube storage box comprises a base (1) made of metal, a drawer (2) arranged on the base (1), a first box body (3) and a second box body (4) arranged at the top of the base (1), wherein the drawer (2), the first box body (3) and the second box body (4) are used for storing ice cubes; a knife rest mechanism (5) is arranged between the first box body (3) and the second box body (4), and the knife rest mechanism (5) is connected to the first box body (3) and the second box body (4) in a sliding manner;

still be provided with objective table (11) on base (1), first box (3) and second box (4) are located the both sides of objective table (11), the other both sides of objective table (11) are provided with first guiding mechanism (6) and first baffle (12) respectively, first guiding mechanism (6) and first baffle (12) are used for the centre gripping rat brain.

2. The apparatus for slicing a brain of a frozen rat according to claim 1, wherein a second adjusting mechanism (7) is further provided on the base (1), and the second box (4) is slidably connected to the second adjusting mechanism (7).

3. The device for slicing a brain of a frozen rat according to claim 1 or 2, wherein a first slide rail (31) is provided on the first box (3), and a second slide rail (41) is provided on the second box (4); the tool rest mechanism (5) is connected to the first sliding rail (31) and the second sliding rail (41) in a sliding manner;

the blade holder mechanism (5) includes: the device comprises a first upright post (51), a second upright post (52) and a cross bar (53), wherein the first upright post (51) is connected to a first slide rail (31) in a sliding manner and is detachably connected with the first slide rail (31), and the second upright post (52) is connected to a second slide rail (41) in a sliding manner and is detachably connected with the second slide rail (41); first pole setting (511) are arranged in first stand column (51) in a slidable manner, second pole setting (521) are arranged in second stand column (52) in a slidable manner, spring (54) are sleeved on first pole setting (511) and second pole setting (521), the tops of first pole setting (511) and second pole setting (521) are connected with cross rod (53), the bottoms of first pole setting (511) and second pole setting (521) are connected with blade mounting seats (55), blades (57) are mounted on blade mounting seats (55), knife grooves (56) are arranged on the lower portions of first stand column (51) and second stand column (52), and knife blades (57) are arranged in knife grooves (56).

4. The device for slicing a brain of a frozen rat according to claim 3, wherein a first sliding block (512) is arranged on the first upright post (51), the first sliding block (512) is slidably connected to the first sliding rail (31), a second sliding block (522) is arranged on the second upright post (52), and the second sliding block (522) is slidably connected to the second sliding rail (41); the first sliding block (512) is provided with a first nut (513), the first nut (513) is used for fixing the first sliding block (512) on the first sliding rail (31), the second sliding block (522) is provided with a second nut (523), and the second nut (523) is used for fixing the second sliding block (522) on the second sliding rail (41).

5. The device for slicing the brain of a frozen rat as claimed in claim 3, wherein a sliding groove (531) is formed at one end of the cross bar (53) close to the second upright post (52), a sliding rod (58) is fixedly arranged at the top of the second upright post (521), and the sliding rod (58) is slidably connected in the sliding groove (531).

6. The device for slicing frozen rat brain according to claim 3, wherein the first slide rail (31) and the second slide rail (41) are provided with scales (8), the first upright post (51) and the second upright post (52) are provided with grooves (59), and the grooves (59) are aligned with the blades (57).

7. The device for slicing frozen rat brain according to claim 1, wherein the first adjusting mechanism (6) comprises: fix fixed block (61) on base (1), pass first screw rod (62) of fixed block (61), swing joint in second baffle (63) on base (1), during the section, first screw rod (62) are close to the one end of objective table (11) and withstand second baffle (63).

8. The device for slicing frozen rat brain according to claim 2, wherein the second adjusting mechanism (7) comprises: the third sliding rail (71), a second screw (72) arranged in the third sliding rail (71) and a third baffle (73) arranged at one end, far away from the first box body (3), of the third sliding rail (71), the second screw (72) penetrates through the third baffle (73) to be connected with the third sliding rail (71), and the second box body (4) is connected to the third sliding rail (71) in a slidable mode.

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