SUMMERY OF THE UTILITY MODEL
The utility model provides a sample processing and culturing device for aquatic product detection, which is simple and convenient to operate, saves time and is operated by sample pretreatment and bacterial colony culture, and overcomes the defects of the prior art.
The technical scheme of the utility model is that:
a sample processing and culturing device for aquatic product detection comprises a main culture box, a diluter main machine and a rotating frame; the front part of the main incubator is provided with a chamber door which is arranged in a sliding way, and the chamber door is provided with an observation window; the lower surface of the main incubator is fixedly connected with a base plate, the front side of the main incubator is provided with a diluter host, the upper surface of the diluter host is provided with a liquid inlet pipe and a liquid outlet pipe, the middle part of the upper surface of the diluter host is provided with a control display screen, the front part of the upper surface of the diluter host is provided with a control button, the base plate close to the diluter host is provided with a rotating frame, and a sample aseptic bag for dilution is placed on the rotating frame; the sample treatment and culture device for aquatic product detection further comprises a fungus bag containing device and a vortex oscillator; the vortex oscillator is arranged close to the rotating frame and is installed on the base plate, and the fungus bag containing device comprises a cylinder and a cover; the one end of cylinder is inwards processed to have the cavity, and the inside processing of another terminal surface of cylinder has the socket joint with vortex oscillator looks adaptation, and the fungus bag holds the device and installs on the vortex oscillator through the socket joint, is equipped with a plurality of boxes that can hold aseptic bag of sample in the cavity, and the upper end edge of cylinder passes through the hinge and is connected with the lid.
The utility model discloses compare prior art's beneficial effect is:
the utility model utilizes the bacteria bag containing device, the aseptic bag mixed with the sample and the diluent is arranged in the box in the bacteria bag containing device, the bacteria bag containing device is inserted on the fitting part of the prior vortex oscillator through the socket joint at the bottom, the vortex oscillation mixing can be started by holding the device, the sample in the device can be fully mixed to achieve the purpose of homogenization, after the vortex oscillation mixing is finished, the device is taken down and put on the experiment table, the lock catch is loosened, the cover is opened, the aseptic bag after the homogenization can be taken out, the structure replaces the traditional operation mode of homogenizing by utilizing the stirrer, the sample is homogenized through the stirrer, the sample can inevitably contact with the stirrer, even if the bacteria bag is repeatedly sterilized, the aseptic stirring can not be realized, the operation is complex and the aseptic requirement can not be reached, the operation of homogenizing the sample is simple and convenient through the bacteria bag containing device and the vortex oscillator, but also can meet the requirement of sterility. Also overcome the gear and stirred the vibrations of the single direction that the vibrations pole brought, the poor drawback of homogeneity effect, through controlling the dilution multiple that the button adjusted needs, through the diluter host computer of work, the diluent flows along the drain pipe automatically, flows into the aseptic bag that is equipped with the sample in advance, and operating time is saved to this kind of operating method, has avoided instability and secondary pollution that manual operation brought, the utility model discloses sample processing culture apparatus is multiple functional, can accomplish to prepare two key operations consuming time and energy of sample in-process dilution and homogeneity, collection sample preliminary treatment, bacterial colony culture operation integration work, improved working method, be favorable to improving aquatic products detection operating efficiency.
The invention will be further described with reference to the accompanying drawings and embodiments:
Detailed Description
Referring to fig. 1 and 2, a sample processing and culturing device for detecting aquatic products according to the present embodiment includes a main incubator 1, a dilutermain unit 2, and a rotatingrack 3; the front part of the main incubator 1 is provided with a chamber door 1-1, the chamber door 1-1 is arranged in a sliding way, and the chamber door 1-1 is provided with an observation window 1-2; the lower surface of the main incubator 1 is fixedly connected with a backing plate 4, the front side of the main incubator 1 is provided with adiluter host 2, the upper surface of thediluter host 2 is provided with a liquid inlet pipe 2-1 and a liquid outlet pipe 2-2, the middle part of the upper surface of thediluter host 2 is provided with an operation display screen 2-3, the front part of the upper surface of thediluter host 2 is provided with an operation button 2-4, the backing plate 4 adjacent to thediluter host 2 is provided with a rotatingframe 3, and a sample sterile bag for dilution is placed on the rotatingframe 3;
the sample treatment and culture device for aquatic product detection further comprises a fungusbag containing device 5 and avortex oscillator 6; thevortex oscillator 6 is arranged adjacent to the rotatingframe 3 and is installed on the base plate 4, and the fungusbag containing device 5 comprises a cylinder 5-1 and a cover 5-2; a cavity 5-3 is formed in one end face of the cylinder 5-1 in an inward mode, a sleeve joint 5-4 matched with thevortex oscillator 6 is formed in the other end face of the cylinder 5-1 in an inward mode, the fungusbag containing device 5 is installed on thevortex oscillator 6 through the sleeve joint 5-4, a plurality ofboxes 7 capable of containing aseptic bags of samples are arranged in the cavity 5-3, and the edge of the upper end of the cylinder 5-1 is connected with the cover 5-2 through ahinge 8. Thebox 7 in the above embodiment plays a role in protecting the aseptic bag, and prevents direct contact with the column 1 or mutual collision in the vibration process, so as to avoid damage and influence on the integrity of the bag.
The dilution technique of the diluent in the above embodiment is related art CN 206940891U.
The main incubator 1 is used for cultivating pretreated aquatic product sample flora, the front part of the main incubator 1 is provided with a chamber door 1-1 which is arranged in a side-sliding manner, a culture dish is taken and placed into the main incubator 1 through opening and closing the chamber door 1-1, the chamber door 1-1 is provided with an observation window 1-2, the condition in the main incubator 1 can be observed at any time through the observation window 1-2, the lower surface of the main incubator 1 is fixedly connected with a backing plate 4, the backing plate 4 enables the main incubator 1, arotating frame 3, a fungusbag containing device 5 and an existingvortex oscillator 6 to be in the same plane, so that the operation of a tester is convenient, the tester can be assisted to improve the working efficiency, the front side of the main incubator 1 is provided with adiluter host 5, the upper surface of thediluter host 5 is provided with a liquid inlet pipe 2-1, and a diluent enters thediluter host 5 through the liquid inlet, the upper surface of the dilutermain machine 5 is also provided with a liquid outlet pipe 2-2, the middle part of the upper surface of the dilutermain machine 2 is provided with an operation display screen 2-3, the front part of the upper surface of the dilutermain machine 2 is also provided with an operation key 2-4, the required dilution times are adjusted by operating the control buttons 2-4, the dilution liquid automatically flows out through the liquid outlet pipe 2-3 and flows into a sterile bag filled with samples after working through thediluter host machine 2, simple and convenient operation, saves the operation time, greatly improves the sterility degree compared with the manual dilution operation, by rotating the rotary disc 3-3 of the rotatingframe 3, the test personnel can conveniently contain the diluent in the sterile bag which is arranged on the rotating frame and is filled with the sample, the operation is convenient, through the homogenization system that fungus bag helddevice 5 andcurrent vortex oscillator 6 constitutes, the sample intensive mixing in order to reach the purpose of homogeneity in the aseptic bag. The dilutermain machine 2 can also adopt a D-50 precision dilution liquid distributor in the existing market, and the VORTEX oscillator can adopt a VORTEX series VORTEX mixer in the market.
Preferably, as shown in FIG. 1, the column 5-1 is a cylinder. Easily obtained materials and convenient processing and operation. Generally, referring to fig. 1, the rotatingframe 3 comprises a base 3-1, a support rod 3-2 and a rotating disc 3-3, the base 3-1 is installed on a base plate 4, the support rod 3-2 is fixedly connected to the upper surface of the base 3-1, the upper end of the support rod 3-2 is connected with the rotating disc 3-3 through a bearing, and a rectangular blind accommodating hole 3-4 is formed in the upper surface of the rotating disc 3-3. The rectangular containing blind holes 3-4 are convenient for taking and placing aseptic bags, the aseptic bags containing samples are placed in the containing blind holes 3-4, a tester rotates the rotary table 3-3 to turn the aseptic bag without diluent to the front of the tester, the rotary table 3-3 is continuously rotated after the diluent is injected, and the diluent is injected into the next aseptic bag, so that the continuous circulation is realized, the operation is convenient, the time and the labor are saved, and the injection efficiency of the diluent is also improved.
Further, as shown in FIG. 3, thecartridge 7 is adhered to the bottom or side of the chamber 5-3. Thebox 7 can be a hard plastic open box, the materials are convenient to obtain, the box is cheap and easy to obtain, and 6 or 7 boxes or the number of theboxes 7 is matched with the volume of the column 5-1. Alternatively, as shown in fig. 4, the bottom surface of the chamber 5-3 is formed with a groove in which thecartridge 7 is placed. The recess of processing certain degree of depth, convenient fixedbox 7,box 7 at this moment can take the square open box of rigid plastic preparation, has changed the fixed mode ofbox 7, also conveniently gets to put and change, andbox 7 at this moment can save to bond fixedly. Thebox 7 in the above embodiment plays a role in protecting the aseptic bag, and prevents direct contact with the column 1 or mutual collision in the vibration process, so as to avoid damage and influence on the integrity of the bag.
As shown in fig. 2, in order to ensure that the lid 5-2 firmly covers the column 5-1, a latch 5-5 is arranged at the side wall of the lid 5-2 opposite to the hinge, a hemispherical protrusion 5-6 is arranged at the outer side wall of the column 5-1, and the latch 5-5 is fastened with the protrusion 5-6 when the lid 5-2 is in a closed state. So set up, be convenient for when aseptic bag vibrations, can not lead to the lid to open because of vibrations, influence homogeneity work.
An operator firstly adjusts the required dilution multiple through the control key 2-4, the dilutermain machine 2 works, the control display screen 2-3 can display relevant parameters, the diluent automatically flows out from the liquid outlet pipe 2-2 and flows into a sterile bag filled with samples, the aseptic bag without the diluent is transferred to an operator by rotating the rotary disc 3-3, the rotary disc 3-3 is continuously rotated after the diluent is injected, the diluent is injected into the next aseptic bag, so circulation operation, the back in diluent and the aseptic bag of sample addition, holdsdevice 5 with the fungus bag and installs onvortex oscillator 6, places aseptic bag in fungus bag holdsbox 7 in thedevice 5, startsvortex oscillator 6 work, holds the aseptic bag vibrations inbox 7 the inside, and its inside sample can obtain abundant mixing with the diluent, reaches the purpose of homogeneity.
The present invention has been disclosed in the above embodiments, but not limited to the embodiments of the present invention, and any person skilled in the art can easily modify, change and modify the above embodiments according to the technical spirit of the present invention without departing from the scope of the present invention.