CN211005418U - Multifunctional stem cell culture plate - Google Patents

Abstract

Translated fromChinese

本实用新型公开了一种干细胞多功能培养板，底板、围板和分隔结构构成，分隔结构又是由括第一板、第二板、挡板、弹簧和拉杆构成，将培养基放置在第一板、第二板和挡板内，干细胞在此培养基内生长，并由弹簧的弹力使得第一板和第二板抵持，当随着干细胞的成长需要分区时，将拉杆向外拉，克服弹簧的弹力，使得第一板和第二板之间分开，一部分干细胞从第一板和第二板之间的缝隙掉入底板上，最后松开拉杆，在弹簧的弹力作用下，第一板和第二板复位，避免了干细胞分区时与外界器械接触，从而获得干细胞免受污染的效果。

The utility model discloses a multifunctional culture plate for stem cells, which is composed of a bottom plate, a surrounding plate and a partition structure. In the first plate, the second plate and the baffle, the stem cells grow in this medium, and the first plate and the second plate are held against each other by the elastic force of the spring. When the division is required with the growth of the stem cells, the pull rod is pulled outward. , overcome the elastic force of the spring, so that the first plate and the second plate are separated, a part of the stem cells fall into the bottom plate from the gap between the first plate and the second plate, and finally release the lever, under the elastic force of the spring, the first plate The first plate and the second plate are reset to avoid contact with external instruments when the stem cells are partitioned, so as to obtain the effect of preventing the stem cells from contamination.

Description

Translated fromChinese

一种干细胞多功能培养板A stem cell multifunctional culture plate

技术领域technical field

本实用新型涉及细胞培养技术领域，尤其涉及一种干细胞多功能培养板。The utility model relates to the technical field of cell culture, in particular to a stem cell multifunctional culture plate.

背景技术Background technique

干细胞是一类具有自我更新的多潜能细胞，在一定条件下可以分化成多种功能细胞，干细胞为组织工程应用提供种子细胞和为探索人类发育奥秘的有效工具，在基础研究和临床应用上都有着巨大的前景。Stem cells are a class of self-renewing pluripotent cells that can differentiate into a variety of functional cells under certain conditions. Stem cells provide seed cells for tissue engineering applications and an effective tool for exploring the mysteries of human development, both in basic research and clinical applications. There are huge prospects.

在培养干细胞时，可以采用干细胞培养板，在培养板内放置培养基进行干细胞培养，随着干细胞的长大，需要进行分区来扩大干细胞的成长空间，存在分区时干细胞易受污染的问题。When culturing stem cells, a stem cell culture plate can be used, and a culture medium can be placed in the culture plate for stem cell culture. As the stem cells grow, they need to be partitioned to expand the growth space of the stem cells. There is a problem that the stem cells are easily contaminated during partitioning.

实用新型内容Utility model content

本实用新型的目的在于提供一种干细胞多功能培养板，旨在解决现有技术中干细胞易受污染的问题。The purpose of the utility model is to provide a multifunctional culture plate for stem cells, which aims to solve the problem that stem cells are easily polluted in the prior art.

为实现上述目的，本实用新型采用的一种干细胞多功能培养板，包括底板、围板和分隔结构，所述围板与所述底板固定连接，并围合所述底板，所述分隔结构与所述围板固定连接，并位于所述围板的内侧，所述分隔结构包括第一板、第二板、挡板、弹簧和拉杆，所述第一板的一端与所述围板固定连接，并位于所述围板的内侧，所述第一板的宽度大小等于所述围板的内径宽度大小，所述第二板与所述第一板抵持，并位于所述第一板与所述围板之间，所述挡板与所述第二板固定连接，并位于所述第二板远离所述底板的一侧，且位于所述第二板远离所述第一板的一端，所述弹簧的一端与所述第二板固定连接，所述弹簧的另一端与所述围板固定连接，所述拉杆穿过所述围板，并与所述围板滑动连接，所述拉杆的一端与所述第二板固定连接，并位于所述第二板远离所述第一板的一侧，所述拉杆的另一端伸出所述围板。In order to achieve the above purpose, a stem cell multifunctional culture plate adopted by the present invention includes a bottom plate, a surrounding plate and a separation structure, the surrounding plate is fixedly connected with the bottom plate, and encloses the bottom plate, and the separation structure is connected with the bottom plate. The enclosure plate is fixedly connected and located inside the enclosure plate, the partition structure includes a first plate, a second plate, a baffle plate, a spring and a pull rod, and one end of the first plate is fixedly connected to the enclosure plate , and is located on the inner side of the enclosure plate, the width of the first plate is equal to the width of the inner diameter of the enclosure plate, the second plate abuts against the first plate, and is located between the first plate and the Between the enclosure plates, the baffle is fixedly connected to the second plate, and is located at the side of the second plate away from the bottom plate, and at the end of the second plate away from the first plate , one end of the spring is fixedly connected to the second plate, the other end of the spring is fixedly connected to the enclosure plate, the pull rod passes through the enclosure plate, and is slidably connected to the enclosure plate, the One end of the pull rod is fixedly connected with the second plate and is located on the side of the second plate away from the first plate, and the other end of the pull rod protrudes from the enclosure plate.

其中，所述拉杆包括第一杆、第二杆和手柄，所述第一杆的一端与所述第二板固定连接，并位于所述第二板远离所述第一板的一端，所述第二杆的另一端与所述围板滑动连接，并伸出所述围板，所述第二杆的一端的所述第二板固定连接，并位于所述第二板远离所述第一板的一端，所述第二杆的另一端与所述围板滑动连接，并伸出所述围板，所述弹簧位于所述第一杆与所述第二杆之间，所述手柄的一端与所述第一杆固定连接，所述手柄的另一端与所述第二杆固定连接，所述手柄位于所述围板的外侧。Wherein, the pull rod includes a first rod, a second rod and a handle, one end of the first rod is fixedly connected with the second plate, and is located at the end of the second plate away from the first plate, the The other end of the second rod is slidably connected to the enclosure plate and protrudes from the enclosure plate, and one end of the second rod is fixedly connected to the second plate and is located at the second plate away from the first plate One end of the plate, the other end of the second rod is slidably connected to the enclosure plate, and extends out of the enclosure plate, the spring is located between the first rod and the second rod, and the handle One end is fixedly connected to the first rod, the other end of the handle is fixedly connected to the second rod, and the handle is located outside the enclosure plate.

其中，所述分隔结构还包括第一橡胶套，所述第一橡胶套与所述第一板固定连接，并位于所述第一板与所述第二板之间。Wherein, the partition structure further includes a first rubber sleeve, the first rubber sleeve is fixedly connected with the first plate, and is located between the first plate and the second plate.

其中，所述分隔结构还包括第二橡胶套，所述第二橡胶套与所述第二板固定连接，并位于所述第一橡胶套与所述第二板之间。Wherein, the partition structure further includes a second rubber sleeve, the second rubber sleeve is fixedly connected with the second plate, and is located between the first rubber sleeve and the second plate.

其中，所述培养板还包括盖板，所述盖板与所述围板铰接，并位于所述分隔结构远离所述底板的一侧。Wherein, the culture plate further includes a cover plate, the cover plate is hinged with the enclosure plate, and is located on the side of the partition structure away from the bottom plate.

其中，所述盖板包括绝缘层、第一导电编织层、第二导电编织层和电源，所述绝缘层与所述围板铰接，并位于所述围板远离所述底板的一端，所述绝缘层上具有气孔，所述气孔的数量为多个，多个所述气孔均匀分布在所述绝缘层上，所述第一导电编织层与所述绝缘层固定连接，并位于所述绝缘层朝向所述分隔结构的一侧，所述第二导电编织层与所述绝缘层固定连接，并位于所述绝缘层背离所述第一导电编织层的一侧，所述电源与所述绝缘层固定连接，并与所述第一导电编织层和所述第二导线编织层电性连接。Wherein, the cover plate includes an insulating layer, a first conductive braided layer, a second conductive braided layer and a power source, the insulating layer is hinged to the enclosure plate, and is located at an end of the enclosure plate away from the bottom plate, the There are air holes on the insulating layer, the number of the air holes is multiple, the plurality of air holes are evenly distributed on the insulating layer, the first conductive braided layer is fixedly connected with the insulating layer, and is located in the insulating layer Facing the side of the separation structure, the second conductive braided layer is fixedly connected to the insulating layer, and is located on the side of the insulating layer away from the first conductive braided layer, and the power supply is connected to the insulating layer It is fixedly connected and electrically connected to the first conductive braided layer and the second conductive braided layer.

其中，所述围板上具有注射孔，所述注射孔的数量至少为两个，并分别位于所述分隔结构的两侧。Wherein, the enclosure plate has injection holes, the number of the injection holes is at least two, and the injection holes are respectively located on both sides of the partition structure.

其中，所述培养板还具有滑板，所述滑板的一端与所述第一板固定连接，并位于所述第一板与所述底板之间。Wherein, the culture plate further has a sliding plate, one end of the sliding plate is fixedly connected with the first plate, and is located between the first plate and the bottom plate.

其中，所述培养板还包括开关，所述开关与所述围板铰接，并经所述注射孔遮住。Wherein, the culture plate further includes a switch, the switch is hinged with the enclosure and covered by the injection hole.

本实用新型的一种干细胞多功能培养板，通过由底板、围板和分隔结构构成，所述分隔结构又是由括第一板、第二板、挡板、弹簧和拉杆构成，将培养基放置在所述第一板、所述第二板和所述挡板内，干细胞在此培养基内生长，并由所述弹簧的弹力使得所述第一板和所述第二板抵持，当随着所述干细胞的成长需要分区时，将所述拉杆向外拉，克服所述弹簧的弹力，使得所述第一板和所述第二板之间分开，一部分干细胞从所述第一板和所述第二板之间的缝隙掉入所述底板上，最后松开所述拉杆，在所述弹簧的弹力作用下，所述第一板和所述第二板复位，避免了干细胞分区时与外界器械接触，从而获得干细胞免受污染的效果。The multifunctional culture plate for stem cells of the utility model is composed of a bottom plate, a surrounding plate and a partition structure, and the partition structure is composed of a first plate, a second plate, a baffle plate, a spring and a pull rod. placed in the first plate, the second plate and the baffle, the stem cells grow in this medium, and the first plate and the second plate are held against by the elastic force of the spring, When it is necessary to partition with the growth of the stem cells, the pull rod is pulled outward to overcome the elastic force of the spring, so that the first plate and the second plate are separated, and a part of the stem cells are separated from the first plate. The gap between the plate and the second plate falls into the bottom plate, and finally the pull rod is released. Under the elastic force of the spring, the first plate and the second plate are reset to avoid stem cells. Contact with external instruments during partitioning, so as to obtain the effect of preventing stem cells from contamination.

附图说明Description of drawings

为了更清楚地说明本实用新型实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本实用新型的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the following briefly introduces the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description These are just some embodiments of the present invention, and for those of ordinary skill in the art, other drawings can also be obtained from these drawings without creative effort.

图1是本实用新型培养板的结构示意图；Fig. 1 is the structural representation of the culture plate of the present utility model;

图2是本实用新型的内部结构示意图；Fig. 2 is the internal structure schematic diagram of the present utility model;

图3是实用新型注射孔处的结构示意图；Fig. 3 is the structural representation at the injection hole of the utility model;

图4是本实用新型盖板的结构示意图；Fig. 4 is the structural representation of the cover plate of the present invention;

图中：100-培养板、10-底板、20-围板、21-注射孔、30-分隔结构、31-第一板、32-第二板、33-挡板、34-弹簧、35-拉杆、351-第一杆、352-第二杆、353- 手柄、36-第一橡胶套、37-第二橡胶套、40-盖板、41-绝缘层、411-气孔、42-第一导电编织层、43-第二导电编织层、44-电源、50-滑板、60-开关。In the figure: 100-culture plate, 10-bottom plate, 20-surrounding plate, 21-injection hole, 30-separation structure, 31-first plate, 32-second plate, 33-baffle plate, 34-spring, 35- Pull rod, 351-first rod, 352-second rod, 353-handle, 36-first rubber sleeve, 37-second rubber sleeve, 40-cover plate, 41-insulation layer, 411-air hole, 42-first Conductive braided layer, 43-second conductive braided layer, 44-power supply, 50-slide plate, 60-switch.

具体实施方式Detailed ways

下面详细描述本实用新型的实施例，所述实施例的示例在附图中示出，其中自始至终相同或类似的标号表示相同或类似的元件或具有相同或类似功能的元件。下面通过参考附图描述的实施例是示例性的，旨在用于解释本实用新型，而不能理解为对本实用新型的限制。The following describes in detail the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary, and are intended to be used to explain the present invention, but should not be construed as a limitation of the present invention.

在本实用新型的描述中，需要理解的是，术语“长度”、“宽度”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本实用新型和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本实用新型的限制。此外，在本实用新型的描述中，“多个”的含义是两个或两个以上，除非另有明确具体的限定。In the description of the present invention, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical" , "horizontal", "top", "bottom", "inside", "outside" and other indicated orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, only for the convenience of describing the present utility model and simplifying the description, Rather than indicating or implying that the indicated device or element must have a particular orientation, be constructed and operate in a particular orientation, it should not be construed as a limitation of the present invention. In addition, in the description of the present invention, "plurality" means two or more, unless otherwise expressly and specifically defined.

请参阅图1至图4，本实用新型提供了一种干细胞多功能培养板100，包括底板10、围板20和分隔结构30，所述围板20与所述底板10固定连接，并围合所述底板10，所述分隔结构30与所述围板20固定连接，并位于所述围板20 的内侧，所述分隔结构30包括第一板31、第二板32、挡板33、弹簧34和拉杆 35，所述第一板31的一端与所述围板20固定连接，并位于所述围板20的内侧，所述第一板31的宽度大小等于所述围板20的内径宽度大小，所述第二板32与所述第一板31抵持，并位于所述第一板31与所述围板20之间，所述挡板33 与所述第二板32固定连接，并位于所述第二板32远离所述底板10的一侧，且位于所述第二板32远离所述第一板31的一端，所述弹簧34的一端与所述第二板32固定连接，所述弹簧34的另一端与所述围板20固定连接，所述拉杆35 穿过所述围板20，并与所述围板20滑动连接，所述拉杆35的一端与所述第二板32固定连接，并位于所述第二板32远离所述第一板31的一侧，所述拉杆35 的另一端伸出所述围板20。Please refer to FIG. 1 to FIG. 4 , the present invention provides a stem cellmultifunctional culture plate 100 , which includes abottom plate 10 , anenclosure plate 20 and apartition structure 30 . Theenclosure plate 20 is fixedly connected to thebottom plate 10 and encloses the In thebottom plate 10, thepartition structure 30 is fixedly connected with theenclosure plate 20 and is located inside theenclosure plate 20. Thepartition structure 30 includes afirst plate 31, asecond plate 32, abaffle 33, aspring 34 and atie rod 35, one end of thefirst plate 31 is fixedly connected to theenclosure plate 20 and is located inside theenclosure plate 20, the width of thefirst plate 31 is equal to the inner diameter of theenclosure plate 20 size, thesecond plate 32 abuts against thefirst plate 31 and is located between thefirst plate 31 and theenclosure plate 20, thebaffle 33 is fixedly connected to thesecond plate 32, and is located on the side of thesecond plate 32 away from thebottom plate 10 and at the end of thesecond plate 32 away from thefirst plate 31 , and one end of thespring 34 is fixedly connected to thesecond plate 32 , the other end of thespring 34 is fixedly connected with theenclosure plate 20 , thepull rod 35 passes through theenclosure plate 20 and is slidably connected with theenclosure plate 20 , and one end of thepull rod 35 is connected with the second Theplate 32 is fixedly connected and located on the side of thesecond plate 32 away from thefirst plate 31 , and the other end of thepull rod 35 protrudes from theenclosure plate 20 .

在本实施方式的实施例中，将所述培养板100放置在温度适合干细胞生长的室内，在进行干细胞培育的初期，将培养基放置在所述第一板31、所述第二板32和所述挡板33围合而成的凹槽内，在所述弹簧34的弹力作用下保持所述第一板31与所述第二板32之间的紧密贴合，随着干细胞的生长，干细胞的体积增大后需要进行分区，此时需要由操作人员握持所述拉杆35，克服所述弹簧 34的弹力、将所述拉杆35向外拉动，所述拉杆35带动所述第二板32与所述第一板31分开，一部分干细胞从所述第一板31和所述第二板32之间的缝隙掉入所述底板10上，一部分干细胞留在所述第一板31和所述第二板32上，并可根据需要添加培养基，最后松开所述拉杆35，在所述弹簧34的弹力作用下，所述第一板31和所述第二板32复位，避免了干细胞分区时与外界器械接触，从而获得干细胞免受污染的效果。In the example of this embodiment, theculture plate 100 is placed in a room with a temperature suitable for stem cell growth, and in the early stage of stem cell culture, the culture medium is placed on thefirst plate 31 , thesecond plate 32 and the In the groove enclosed by thebaffle plate 33, thefirst plate 31 and thesecond plate 32 are kept in close contact under the elastic force of thespring 34. As the stem cells grow, After the volume of stem cells increases, it needs to be partitioned. At this time, the operator needs to hold thepull rod 35, overcome the elastic force of thespring 34, and pull thepull rod 35 outward, and thepull rod 35 drives the second plate. 32 is separated from thefirst plate 31, a part of the stem cells fall into thebottom plate 10 from the gap between thefirst plate 31 and thesecond plate 32, and a part of the stem cells remain in thefirst plate 31 and thesecond plate 32. Thesecond plate 32 is placed on thesecond plate 32, and culture medium can be added as required, and finally thepull rod 35 is released. Under the elastic force of thespring 34, thefirst plate 31 and thesecond plate 32 are reset, avoiding the When the stem cells are partitioned, they come into contact with external instruments, so as to obtain the effect of preventing the stem cells from contamination.

进一步地，所述拉杆35包括第一杆351、第二杆352和手柄353，所述第一杆351的一端与所述第二板32固定连接，并位于所述第二板32远离所述第一板31的一端，所述第二杆352的另一端与所述围板20滑动连接，并伸出所述围板20，所述第二杆352的一端的所述第二板32固定连接，并位于所述第二板32远离所述第一板31的一端，所述第二杆352的另一端与所述围板20滑动连接，并伸出所述围板20，所述弹簧34位于所述第一杆351与所述第二杆352 之间，所述手柄353的一端与所述第一杆351固定连接，所述手柄353的另一端与所述第二杆352固定连接，所述手柄353位于所述围板20的外侧。Further, thepull rod 35 includes afirst rod 351, asecond rod 352 and ahandle 353, one end of thefirst rod 351 is fixedly connected with thesecond plate 32, and is located on thesecond plate 32 away from the One end of thefirst plate 31 and the other end of thesecond rod 352 are slidably connected to theenclosure plate 20 and extend out of theenclosure plate 20 , and thesecond plate 32 at one end of thesecond rod 352 is fixed connected and located at one end of thesecond plate 32 away from thefirst plate 31, the other end of thesecond rod 352 is slidably connected with theenclosure plate 20 and extends out of theenclosure plate 20, thespring 34 is located between thefirst rod 351 and thesecond rod 352, one end of thehandle 353 is fixedly connected to thefirst rod 351, and the other end of thehandle 353 is fixedly connected to thesecond rod 352 , thehandle 353 is located outside theenclosure plate 20 .

在本实施方式中，通过所述第一杆351和所述第二杆352的设置，可以使得所述第二板32受到拉力更加平稳，也避免拉动时所述第二板32发生旋转运动，通过手柄353，可以使得更好受力。In this embodiment, through the arrangement of thefirst rod 351 and thesecond rod 352, thesecond plate 32 can be pulled more stably, and thesecond plate 32 can be prevented from rotating during pulling. Through thehandle 353, better force can be applied.

进一步地，所述分隔结构30还包括第一橡胶套36、所述第一橡胶套36与所述第一板31固定连接，并位于所述第一板31与所述第二板32之间。在本实施方式的实施例中，通过所述第一橡胶套36，在所述弹簧34的弹力作用下，所述第一橡胶套36夹持在所述第一板31和所述第二板32之间，所述第一橡胶套 36受到挤压，所述第一橡胶套36变形产生一个对所述第一板31和所述第二板 32接触端口的弹力，起到增强所述第一板31和所述第二板32接触端口密封性目的，避免在干细胞在培育初期时从所述第一板31和所述第二板32之间掉落。Further, thepartition structure 30 further includes afirst rubber sleeve 36 , and thefirst rubber sleeve 36 is fixedly connected to thefirst plate 31 and located between thefirst plate 31 and thesecond plate 32 . In the example of this embodiment, through thefirst rubber sleeve 36 , under the elastic force of thespring 34 , thefirst rubber sleeve 36 is clamped between thefirst plate 31 and thesecond plate 32, thefirst rubber sleeve 36 is squeezed, and the deformation of thefirst rubber sleeve 36 generates an elastic force for the contact ports of thefirst plate 31 and thesecond plate 32, which enhances thefirst plate 31 and thesecond plate 32. The contact ports of thefirst plate 31 and thesecond plate 32 are used for sealing purposes to prevent stem cells from falling out between thefirst plate 31 and thesecond plate 32 at the initial stage of cultivation.

进一步地，所述分隔结构30还包括第二橡胶套37，所述第二橡胶套37与所述第二板32固定连接，并位于所述第一橡胶套36与所述第二板32之间。在本实施方式的实施例中，通过所述第二橡胶套37，在所述弹簧34的弹力作用下，所述第二橡胶套37夹持在所述第一橡胶套36和所述第二板32之间，所述第二橡胶套37受到挤压，所述第二橡胶套37变形产生一个对所述第一橡胶套36和所述第二板32接触端口的弹力，起到增强所述第一板31和所述第二板32接触端口密封性目的，避免培养基在培育初期时从所述第一板31和所述第二板32之间泄漏。Further, thepartition structure 30 further includes asecond rubber sleeve 37 , thesecond rubber sleeve 37 is fixedly connected to thesecond plate 32 and located between thefirst rubber sleeve 36 and thesecond plate 32 . between. In the example of this embodiment, through thesecond rubber cover 37, under the elastic force of thespring 34, thesecond rubber cover 37 is clamped between thefirst rubber cover 36 and thesecond rubber cover 37. Between theplates 32, thesecond rubber sleeve 37 is squeezed, and the deformation of thesecond rubber sleeve 37 generates an elastic force for the contact port of thefirst rubber sleeve 36 and thesecond plate 32, which strengthens the The purpose of sealing the contact ports of thefirst plate 31 and thesecond plate 32 is to prevent the medium from leaking from between thefirst plate 31 and thesecond plate 32 in the early stage of cultivation.

进一步地，所述培养板100还包括盖板40，所述盖板40与所述围板20铰接，并位于所述分隔结构30远离所述底板10的一侧。在本实施方式的实施例中，通过所述盖板40的关闭与打开，将干细胞与外界隔离，避免外界空气对所述干细胞造成污染。Further, theculture plate 100 further includes acover plate 40 , thecover plate 40 is hinged to theenclosure plate 20 and is located on the side of thepartition structure 30 away from thebottom plate 10 . In the example of this embodiment, by closing and opening thecover plate 40, the stem cells are isolated from the outside world, so as to prevent the outside air from polluting the stem cells.

进一步地，所述盖板40包括绝缘层41、第一导电编织层42、第二导电编织层43和电源44，所述绝缘层41与所述围板20铰接，并位于所述围板20远离所述底板10的一端，所述绝缘层41上具有气孔411，所述气孔411的数量为多个，多个所述气孔411均匀分布在所述绝缘层41上，所述第一导电编织层42 与所述绝缘层41固定连接，并位于所述绝缘层41朝向所述分隔结构30的一侧，所述第二导电编织层43与所述绝缘层41固定连接，并位于所述绝缘层41背离所述第一导电编织层42的一侧，所述电源44与所述绝缘层41固定连接，并与所述第一导电编织层42和所述第二导线编织层电性连接。Further, thecover plate 40 includes aninsulating layer 41 , a first conductive braidedlayer 42 , a second conductive braidedlayer 43 and apower source 44 . Theinsulating layer 41 is hinged to theenclosure plate 20 and located on theenclosure plate 20 At one end away from thebottom plate 10 , theinsulating layer 41 hasair holes 411 , and the number of theair holes 411 is multiple. The plurality ofair holes 411 are evenly distributed on theinsulating layer 41 . The first conductive weave Thelayer 42 is fixedly connected to theinsulating layer 41 and is located on the side of theinsulating layer 41 facing theseparation structure 30 , and the second conductive braidedlayer 43 is fixedly connected to theinsulating layer 41 and located on theinsulating layer 41 . On the side of thelayer 41 facing away from the firstconductive braided layer 42 , thepower source 44 is fixedly connected to the insulatinglayer 41 and is electrically connected to the firstconductive braided layer 42 and the second conductive braided layer.

在本实施方式中，在进行干细胞培育以及过滤分离时，启动所述电源44，所述电源44选用脉冲电压发生器，所述电源44与所述绝缘层41固定连接，其工作时的脉冲频率在二十到五十赫兹范围内，所述绝缘层41选用有机纤维、避免生成过高静电积累，所述绝缘层41的厚度在一毫米，避免所述第一导电编织层42和所述第二导电编织层43接触发生断路，所述第一导电编织层42和所述第二导电编织层43都由金属丝编织而成，所述第一导电编织层42和所述第二导电编织层43通电之后，形成了一个外侧为正，内侧为负的静电场，由于空气中的灰尘通常都带正电荷，受到第一导电编织层42的同性排斥阻隔，避免灰尘进入所述空腔内对干细胞造成污染，而空腔内不小心产生的细菌都带负电荷，被吸附到外导电网的表面，在高压静电场的作用下，将细菌、病毒杀死留下干细胞，从而获得免收污染的效果。In this embodiment, thepower source 44 is activated when the stem cells are cultivated and filtered and separated. Thepower source 44 selects a pulse voltage generator. Thepower source 44 is fixedly connected to the insulatinglayer 41, and the pulse frequency during operation is In the range of 20 to 50 Hz, the insulatinglayer 41 is made of organic fibers to avoid excessive electrostatic accumulation, and the thickness of the insulatinglayer 41 is one millimeter to avoid the firstconductive braided layer 42 and the The two conductive braided layers 43 are in contact and open circuit occurs, the firstconductive braided layer 42 and the secondconductive braided layer 43 are both braided by metal wires, the firstconductive braided layer 42 and the secondconductive braided layer 43 After 43 is energized, a positive electrostatic field is formed on the outside and negative on the inside. Since the dust in the air is usually positively charged, it is blocked by the homosexual repulsion of the firstconductive braided layer 42 to prevent dust from entering the cavity. Stem cells cause pollution, and the bacteria accidentally generated in the cavity are negatively charged and are adsorbed to the surface of the outer conductive mesh. Under the action of the high-voltage electrostatic field, the bacteria and viruses are killed and the stem cells are left, so as to avoid pollution. Effect.

进一步地，所述围板20上具有注射孔21，所述注射孔21的数量至少为两个，并分别位于所述分隔结构30的两侧。在本实施方式的实施例中，当干细胞进行分区后，可以通过两所述注射孔21进行培养基的添加，避免添加培养基还要打开所述盖板40，进而避免打开所述盖板40出现外界空气细菌等造成干细胞的受到污染。Further, theenclosure plate 20 has injection holes 21 , the number of the injection holes 21 is at least two, and the injection holes 21 are located on both sides of thepartition structure 30 respectively. In the example of this embodiment, after the stem cells are partitioned, the medium can be added through the twoinjection holes 21 to avoid opening thecover plate 40 when adding medium, thereby avoiding opening thecover plate 40 The appearance of bacteria in the outside air causes the contamination of stem cells.

进一步地，所述培养板100还具有滑板50，所述滑板50的一端与所述第一板31固定连接，并位于所述第一板31与所述底板10之间。在本实施方式的实施例中，所述滑板50倾斜设置，所述滑板50的另一端位于所述第二板32的下方，干细胞从所述第一板31掉落至滑板50上，再由所述滑板50将干细胞引入所述底板10上，减少干细胞分区时的冲击。Further, theculture plate 100 further has a slidingplate 50 , one end of the slidingplate 50 is fixedly connected with thefirst plate 31 and is located between thefirst plate 31 and thebottom plate 10 . In the embodiment of the present embodiment, the slidingplate 50 is disposed obliquely, the other end of the slidingplate 50 is located below thesecond plate 32 , the stem cells are dropped from thefirst plate 31 to the slidingplate 50, The slidingplate 50 introduces stem cells onto thebottom plate 10 to reduce the impact of stem cells partitioning.

进一步地，所述培养板100还包括开关60，所述开关60与所述围板20铰接，并经所述注射孔21遮住。在本实施方式的实施例中，所述开关60为圆形板状，在不需要进行培养基的添加时，所述开关60与所述围板20贴合，所述开关60将所述注射孔21堵住，避免外界细菌进入所述围板20内对干细胞造成污染。Further, theculture plate 100 further includes aswitch 60 , theswitch 60 is hinged with theenclosure plate 20 and covered by theinjection hole 21 . In the example of this embodiment, theswitch 60 is in the shape of a circular plate. When the medium does not need to be added, theswitch 60 is attached to theenclosure plate 20 , and theswitch 60 is used to inject the injection. Theholes 21 are blocked to prevent external bacteria from entering theenclosure 20 and causing contamination of the stem cells.

以上所揭露的仅为本实用新型一种较佳实施例而已，当然不能以此来限定本实用新型之权利范围，本领域普通技术人员可以理解实现上述实施例的全部或部分流程，并依本实用新型权利要求所作的等同变化，仍属于实用新型所涵盖的范围。What has been disclosed above is only a preferred embodiment of the present invention, of course, it cannot limit the scope of rights of the present invention. Those of ordinary skill in the art can understand that all or part of the process of realizing the above-mentioned embodiment can be implemented according to the present invention. The equivalent changes made by the claims of the utility model still belong to the scope covered by the utility model.

Claims (9)

Translated fromChinese

1.一种干细胞多功能培养板，其特征在于，1. a stem cell multifunctional culture plate, is characterized in that,包括底板、围板和分隔结构，所述围板与所述底板固定连接，并围合所述底板，所述分隔结构与所述围板固定连接，并位于所述围板的内侧，所述分隔结构包括第一板、第二板、挡板、弹簧和拉杆，所述第一板的一端与所述围板固定连接，并位于所述围板的内侧，所述第一板的宽度大小等于所述围板的内径宽度大小，所述第二板与所述第一板抵持，并位于所述第一板与所述围板之间，所述挡板与所述第二板固定连接，并位于所述第二板远离所述底板的一侧，且位于所述第二板远离所述第一板的一端，所述弹簧的一端与所述第二板固定连接，所述弹簧的另一端与所述围板固定连接，所述拉杆穿过所述围板，并与所述围板滑动连接，所述拉杆的一端与所述第二板固定连接，并位于所述第二板远离所述第一板的一侧，所述拉杆的另一端伸出所述围板。It includes a bottom plate, an enclosure plate and a partition structure, the enclosure plate is fixedly connected to the bottom plate and encloses the bottom plate, the partition structure is fixedly connected to the enclosure plate, and is located inside the enclosure plate, the The partition structure includes a first plate, a second plate, a baffle plate, a spring and a pull rod. One end of the first plate is fixedly connected to the enclosure plate and is located inside the enclosure plate. The width of the first plate is the same size as equal to the inner diameter and width of the enclosure plate, the second plate abuts the first plate and is located between the first plate and the enclosure plate, and the baffle plate and the second plate are fixed connected and located on the side of the second plate away from the bottom plate, and at the end of the second plate away from the first plate, one end of the spring is fixedly connected with the second plate, the spring The other end of the pull rod is fixedly connected to the enclosure plate, the pull rod passes through the enclosure plate and is slidably connected to the enclosure plate, and one end of the pull rod is fixedly connected to the second plate and is located in the second plate. One side of the plate away from the first plate, and the other end of the pull rod protrudes from the enclosure plate.2.如权利要求1所述的干细胞多功能培养板，其特征在于，所述拉杆包括第一杆、第二杆和手柄，所述第一杆的一端与所述第二板固定连接，并位于所述第二板远离所述第一板的一端，所述第二杆的另一端与所述围板滑动连接，并伸出所述围板，所述第二杆的一端的所述第二板固定连接，并位于所述第二板远离所述第一板的一端，所述第二杆的另一端与所述围板滑动连接，并伸出所述围板，所述弹簧位于所述第一杆与所述第二杆之间，所述手柄的一端与所述第一杆固定连接，所述手柄的另一端与所述第二杆固定连接，所述手柄位于所述围板的外侧。2. The stem cell multifunctional culture plate according to claim 1, wherein the draw rod comprises a first rod, a second rod and a handle, one end of the first rod is fixedly connected to the second plate, and Located at one end of the second plate away from the first plate, the other end of the second rod is slidably connected to the enclosure plate and protrudes from the enclosure plate, and the first end of the second lever The two plates are fixedly connected and located at the end of the second plate away from the first plate. The other end of the second rod is slidably connected to the enclosure plate and extends out of the enclosure plate. The spring is located at the end of the second plate. Between the first rod and the second rod, one end of the handle is fixedly connected to the first rod, the other end of the handle is fixedly connected to the second rod, and the handle is located on the enclosure plate outside.3.如权利要求2所述的干细胞多功能培养板，其特征在于，所述分隔结构还包括第一橡胶套，所述第一橡胶套与所述第一板固定连接，并位于所述第一板与所述第二板之间。3. The multifunctional culture plate for stem cells according to claim 2, wherein the separation structure further comprises a first rubber cover, the first rubber cover is fixedly connected to the first plate, and is located in the first rubber cover. between a plate and the second plate.4.如权利要求3所述的干细胞多功能培养板，其特征在于，所述分隔结构还包括第二橡胶套，所述第二橡胶套与所述第二板固定连接，并位于所述第一橡胶套与所述第二板之间。4. The stem cell multifunctional culture plate according to claim 3, wherein the partition structure further comprises a second rubber cover, the second rubber cover is fixedly connected to the second plate and is located in the second rubber cover. between a rubber sleeve and the second plate.5.如权利要求1所述的干细胞多功能培养板，其特征在于，所述培养板还包括盖板，所述盖板与所述围板铰接，并位于所述分隔结构远离所述底板的一侧。5. The multifunctional culture plate for stem cells according to claim 1, characterized in that, the culture plate further comprises a cover plate, the cover plate is hinged to the enclosure plate, and is located at a position where the separation structure is away from the bottom plate. side.6.如权利要求5所述的干细胞多功能培养板，其特征在于，所述盖板包括绝缘层、第一导电编织层、第二导电编织层和电源，所述绝缘层与所述围板铰接，并位于所述围板远离所述底板的一端，所述绝缘层上具有气孔，所述气孔的数量为多个，多个所述气孔均匀分布在所述绝缘层上，所述第一导电编织层与所述绝缘层固定连接，并位于所述绝缘层朝向所述分隔结构的一侧，所述第二导电编织层与所述绝缘层固定连接，并位于所述绝缘层背离所述第一导电编织层的一侧，所述电源与所述绝缘层固定连接，并与所述第一导电编织层和所述第二导电编织层电性连接。6. The stem cell multifunctional culture plate according to claim 5, wherein the cover plate comprises an insulating layer, a first conductive braided layer, a second conductive braided layer and a power source, and the insulating layer is connected to the enclosure plate hinged and located at the end of the enclosure plate away from the bottom plate, the insulating layer has air holes, the number of the air holes is multiple, and the plurality of air holes are evenly distributed on the insulating layer, the first The conductive braided layer is fixedly connected to the insulating layer and is located on the side of the insulating layer facing the separation structure, and the second conductive braided layer is fixedly connected to the insulating layer and is located on the insulating layer away from the On one side of the first conductive braided layer, the power supply is fixedly connected to the insulating layer, and is electrically connected to the first conductive braided layer and the second conductive braided layer.7.如权利要求6所述的干细胞多功能培养板，其特征在于，所述围板上具有注射孔，所述注射孔的数量至少为两个，并分别位于所述分隔结构的两侧。7 . The multifunctional culture plate for stem cells according to claim 6 , wherein the enclosure plate has injection holes, the number of the injection holes is at least two, and the injection holes are respectively located on both sides of the partition structure. 8 .8.如权利要求1所述的干细胞多功能培养板，其特征在于，所述培养板还具有滑板，所述滑板的一端与所述第一板固定连接，并位于所述第一板与所述底板之间。8. The multifunctional culture plate for stem cells according to claim 1, wherein the culture plate further has a sliding plate, one end of the sliding plate is fixedly connected with the first plate, and is located between the first plate and the second plate. between the bottom plates.9.如权利要求7所述的干细胞多功能培养板，其特征在于，所述培养板还包括开关，所述开关与所述围板铰接，并经所述注射孔遮住。9 . The multifunctional culture plate for stem cells according to claim 7 , wherein the culture plate further comprises a switch, the switch is hinged with the enclosure plate and covered by the injection hole. 10 .

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