One kind being used for electrochemiluminescent immunoassay Magneto separate cleaning deviceTechnical field
Magnetic separation technique field in being reacted the utility model is related to electrochemiluminescent immunoassay, and in particular to one kind being used for electrochemiluminescent immunoassayMagneto separate cleaning device.
Background technology
Electrochemiluminescent immunoassay be using fluorescent material either electrochemiluminescent immunoassay reactant species as marker labelled antigen or anti-Body, sample to be measured and the magnetic particle reaction for being coated with specific antigen or antibody, by the combining marker in magnetic field with it is freeMarker carries out separation cleaning, and luminous promoting agent is then added and carries out luminescence-producing reaction, by the detection of light intensity carry out it is quantitative orQualitative detection.
Currently, existing Magneto separate cleaning device is complicated, process is cumbersome, of high cost, realizes more difficult.
Utility model content
Therefore, the purpose of this utility model is to provide a kind of by realization fluid injection, imbibition, the magnetic suck of rationally arranging, mixingIn the cleaning device of one.
The utility model one kind being used for electrochemiluminescent immunoassay Magneto separate cleaning device, including：
Support board；
Cup tray is reacted, there are annular at least eight reaction cup positions being evenly distributed, the reaction cup tray to be fixed on instituteIt states on support board and can be around its axis rotation；
At least one group of magnets has along the reaction cup tray outer edge side arrangement with reaction cup position correspondingly extremelyFew two magnet；
At least one liquid relief component, have be fixed on fixed frame with the one-to-one injection needle in reaction cup position and imbibitionNeedle passes through fixed frame moving up and down so that injection needle or aspirating needle immigration, removal reaction cup vertically；
At least one set of mixing component has the stirring-head corresponding with reaction cup position set on the group of magnets side；Pass throughThe rotation of the reaction cup tray, realizes the rotation of reaction cup position, to coordinate group of magnets, liquid relief component and mixing component to reactionThe cleaning of reaction cup on cup position.
Optionally, the aspirating needle is long compared with injection needle.
Optionally, the aspirating needle be placed in the corresponding reaction cup of the group of magnets the last one magnet place position just onSide.
Optionally, the fixed frame is moved up and down by lead screw motor control, and the lead screw motor is set under support boardPortion is simultaneously equipped with guiding axis to ensure that fixed frame does not glance off by the lower surface of fixed structure support board, in fixed structure.
Optionally, the reaction cup tray drives synchronous belt to make its rotation by stepper motor by synchronous pulley.
Optionally, it is equipped with reaction cup catch on the top of the correspondence reaction cup position of the stirring-head, the reaction cup catch is solidOn support board.
Optionally, the top of the stirring-head is eccentric structure.
Optionally, cleaning solution is connected to after fluid injection switches valve group by topping-up pump and is then connected to injection needle；It is useless after cleaningLiquid is exported after being drawn by aspirating needle by suction pumps.
Optionally, the aspirating needle and/or injection needle are being equipped with spring structure with the junction of fixed frame.
Optionally, further include cleaning device, clear water is injected by fluid injection switching valve group and is washed in needle pond.
Technical solutions of the utility model have the following advantages that：
It is provided by the utility model a kind of for electrochemiluminescent immunoassay Magneto separate cleaning device, pass through rationally to arrange and realizes variorumLiquid, imbibition, magnetic suck, mixing realize automatic Magneto separate and are cleaned multiple times in one, and structure is simpler, clean cleaner, performanceIt is more stable, it can be used alone to be integrated in full-automatic instrument and use.
Description of the drawings
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior artSpecific implementation mode or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described belowIn attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the artUnder the premise of labour, other drawings may also be obtained based on these drawings.
Fig. 1 and Fig. 2 is electrochemiluminescent immunoassay Magneto separate cleaning device figure；
Fig. 3 is reaction cup tray rotational structure figure；
Fig. 4 is the structural schematic diagram of liquid relief component；
Fig. 5 is the structural schematic diagram of mixing component.
Specific implementation mode
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that describedEmbodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originallyThe every other embodiment that field those of ordinary skill is obtained without making creative work, belongs to this practicalityNovel protected range.
It is in the description of the present invention, it should be noted that term "center", "upper", "lower", "left", "right", " perpendicularDirectly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is onlyThe utility model and simplifying describes for ease of description, do not indicate or imply the indicated device or element must have it is specificOrientation, with specific azimuth configuration and operation, therefore should not be understood as limiting the present invention.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacifiedDress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrallyConnection；It can be mechanical connection, can also be electrical connection；Can be directly connected, can also indirectly connected through an intermediary,It can be the connection inside two elements.For the ordinary skill in the art, it can understand above-mentioned art with concrete conditionThe concrete meaning of language in the present invention.
In addition, as long as technical characteristic involved in the utility model different embodiments disclosed below is each otherNot constituting conflict can be combined with each other.
Embodiment 1
It is provided in this embodiment a kind of for electrochemiluminescent immunoassay Magneto separate cleaning device, shown referring to Fig. 1 and Fig. 2, Magneto separateThe device frame of cleaning is made of horizontal supporting plate 31 and vertical support plate 32, and horizontal supporting plate 31 is by two pieces of vertical support plates 32Support, reaction 13 fitted shaft bearing sleeve 15 of cup tray are fixed on 31 on horizontal supporting plate, and the rotational structure of reaction cup is referring to Fig. 3 institutesShow, reaction cup tray 13 drives synchronous belt 12 to make its rotary motion by stepper motor 11 by synchronous pulley 14, reacts cup tray20 placement positions for being uniformly distributed reaction cup are shared on 13, and next reaction cup position is rotated to every the set time.
Liquid relief component is shown in Figure 4, including injection needle 24 is fixed on aspirating needle 23 on fixed frame 21, and there is bullet in bearingSpring structure 25, can effectively make aspirating needle reach reaction cup bottom, and injection needle 24 is draw point, 23 syringe needle of aspirating needle with aspirating needle 23For angle mode, and aspirating needle 23 is longer than injection needle, in the present embodiment, including 4 aspirating needles 23 and 4 injection needles 24, imbibitionNeedle 23 is positioned over the surface of the last one magnet of group of magnets 61 corresponding reaction cup placement position, first three root injection needle 24 is positioned overAspirating needle 23 corresponds to the surface that reaction cup places position next bit, and latter injection needle 24 is placed on same position with aspirating needle 23,Fixed frame 21 drives its up and down motion by lead screw motor 22, to ensure that it vertically installs two guiding axis in lead screw both sides26 ensure that its verticality, lead screw motor 22 are fixed on by lead screw motor fixed structure 27 on horizontal supporting plate 31.
Specifically, group of magnets 61 is fixed on horizontal supporting plate 31, and group of magnets 61 is that 13 outside of cup tray is reacted successively in edgeThree independent permanent magnets of arrangement, share four groups, are symmetrically placed on 13 outside of reaction cup tray, and each magnet corresponds to oneReaction cup places position, and after placing response cup, reaction cup is fitted closely with magnet.
Specifically, mixing component is shown in Figure 5, and rabbling mechanism 5 is made of stirring-head 51 and direct current generator 52, direct currentStirring-head 51 is driven to rise for machine 52 and rotary motion, rabbling mechanism 5 are fixed on the supporting plate by rabbling mechanism fixed block 55, installationReaction cup is corresponded in first three root injection needle 24 to place immediately below the next position of position, rabbling mechanism 5 can make magnetic bead clear in injectionMixing is shaken after washing lotion；It being detached from rabbling mechanism 5 and not up to mixed effect when to prevent reaction cup earthquake, pacifying on the supporting plateReaction cup catch 54 is filled.In the present embodiment, stirring-head be smooth material, top be eccentric structure, inside have helicla flute,Its spiral and rotary motion are driven by motor, stirring placement position is closer away from injection needle, and magnetic bead is got over cleaning solution mixed effectIt is good.
Then cleaning solution, which by topping-up pump 41 is transferred to rubber tube and is connected to fluid injection switching valve group 71, to be connected to by rubber tubeInjection needle 24, is implanted sequentially reaction cup；Waste liquid is connected to waste liquid needle 23 by rubber tube by waste drains pump 42 while siphoning away, in magneticFirst three root injection needle works during separation cleaning, last root injection needle only works when cleaning device is safeguarded.
24 aspirating needle of injection needle, 23 fixed frame 21 preferably must be held in highest order when reaction cup tray 13 rotates.
It is above-mentioned as follows for electrochemiluminescent immunoassay Magneto separate cleaning operation flow：
The reaction cup that reaction is completed enters 101 on reaction cup tray 13, and reaction cup tray rotates a position, insteadAnswer cup in magnet one unit interval of 611 magnetic suck, reaction cup tray 13 rotates a position, react later cup tray 13 everyOne unit interval rotates a position, and reaction cup rotates one in magnet one unit interval of 612 magnetic suck, reaction cup trayPosition, fixed frame 21 drop to suction range, open suction pumps, while fixed frame 21 is slowly declined to reaction cup bottom, waits for liquidIt sucks rear fixed frame 21 and rises to peak, reaction cup tray 13 rotates a position, and fixed frame 21 drops to fluid injection height, beatsFluid injection switching valve group 711 to be opened, topping-up pump is opened, fluid injection is closed after fluid injection and switches valve group 711, fixed frame 21 rises to peak,It reacts cup tray 13 and rotates a position, open rabbling mechanism 5, stir 3 seconds, reaction cup tray 13 rotates a position, successivelyRepeat above-mentioned action twice, reaction cup moves to 116 on reaction cup tray 13, and reaction cup is in 64 position of group of magnets, 641 magneticA unit interval is adsorbed, reaction cup tray rotates a position, and reaction cup is in 64 position of group of magnets, 642 magnetic suck, one unitTime, reaction cup tray 13 rotate a position, then imbibition, and reaction cup tray 13 rotates a position, and above-mentioned is one anti-Cup cleaning is answered to complete.If realizing that multiple reaction cup cleanings need to only be completed respectively in each reaction cup tray corresponding positionAction.By repeating above-mentioned cleaning process 3 times, you can ensure that magnetic bead can clean up completely, the concussion of reaction cup mixing,Each at least two unit interval of magnetic suck, in turn ensure that magnetic suck is reliable and complete, 23 slow side of aspirating needle is inhaled below, i.e.,The liquid residual on aspirating needle 23 can be reduced, and ensures that magnetic bead is not sucked away.
The present embodiment further includes washing needle pond, is located at below injection needle and aspirating needle, when clean and maintenance, by switching valve clearWater, which is injected into, washes needle pond, aspirating needle soaking and washing repeatedly.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is rightFor those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation orIt changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation orAmong the protection domain that variation is created still in the utility model.