A kind of cow's serum complement elimination constant temperature mixing stirring deviceTechnical field
The utility model is related to cow's serum complement technology for eliminating field, specially a kind of cow's serum complement elimination is stirred with constant temperatureMix mixing arrangement.
Background technology
Complement is the albumen for being present in normal person and animal blood serum and having enzymatic activity after activated with one group in tissue fluidMatter confirms early in Bordet at the end of the 19th century, a kind of heat labile ingredient is contained in new blood, can assist and supplement specificityAntibody, mediated immunity bacteriolyze, haemocylolysis, therefore referred to as complement, complement be by more than 30 kind soluble proteins, membrane-binding albumen andThe polymolecular system of complement receptors composition, therefore referred to as complement system can be by it according to the biological function of each ingredient of complement systemIt is divided into complement proper constituent, complement regulatory ingredient and complement receptors, the wherein energy in ox body of complement system present in cow's serumThe intrusion for resisting allogene is enough played, the complement system in cow's serum adversely affects the cell of other species, meetingCause heterogenous cell form bad, cell quantity proliferation is partially slow, and dead phenomenon even occurs in a small amount of cell culture, causes cellCloning efficiency is relatively low.
Complement elimination process is critically important in process of production for current cow's serum, and temperature is excessively high will be to complement removing methodThe nutrition of middle serum has destruction, and cell is adherent, is proliferated and maintains function that can all be affected, so provide a kind of ox bloodClear complement elimination solves the problems, such as above-mentioned be previously mentioned with constant temperature mixing stirring device.
Utility model content
(1) the technical issues of solving
In view of the deficiencies of the prior art, the utility model provides a kind of cow's serum complement elimination and is stirred dress with constant temperatureIt sets, has the advantages of keeping constant temperature and stirring evenly, solving current cow's serum, complement eliminates process very in process of productionImportant, temperature is excessively high will to have the nutrition of serum in complement removing method destruction, and cell is adherent, is proliferated and maintains functionThe problem of will being affected.
(2) technical solution
For the purpose realized above-mentioned holding constant temperature and stirred evenly, the utility model provides the following technical solutions：A kind of oxSerum complement, which is eliminated, uses constant temperature mixing stirring device, including agitator tank, and there are four supports for the bottom fixed installation of the agitator tankLeg is installed with mounting ring at the center at the top of the agitator tank, stirring motor is installed at the top of the mounting ring,The bottom of the stirring motor output shaft is installed with the drive rod through agitator tank, and the outside of the drive rod is fixedly mountedIt is distributed uniform paddle, the bottom of the drive rod is fixedly mounted scrapes bottom plate, the outside of the drive rod there are four sectorAnd check ring is installed on the inner roof wall of agitator tank, the top of the agitator tank and the left side positioned at stirring motorSide is interspersed to be provided with inlet connector, and the interspersed temperature that is provided in the top of the agitator tank and the right side for being located at stirring motor is visitedNeedle, the top of the agitator tank and the back side for being located at stirring motor, which are interted, is provided with air pressure valve, and the bottom of the agitator tank is wornIt plugs and is equipped with discharge nozzle, the right end of the discharge nozzle is installed with discharging control connector, the lower section on the left of the agitator tankInterspersed to be provided with probe tube, the left end of the probe tube is installed with sampling control joint, and the inside of the agitator tank opens upThere are inner cavity, the inside of the inner cavity to be arranged with heater strip, the inside of the inner cavity is arranged with loop pipe, the right side of the agitator tankIt is installed with circulating pump, the right side of the agitator tank and is installed with condenser below circulating pump, it is described coldConnecting tube is fixedly connected between condenser and circulating pump.
Preferably, one end at the top of the loop pipe is run through agitator tank and is bent downwardly and is connected to circulating pump fixation, instituteOne end of circuit bottom of the tube is stated to be connected to condenser fixation through agitator tank and being bent upwards.
Preferably, one end of the temperature probe bottom is run through and extends to the inside of agitator tank.
Preferably, the probe tube is located at the lower section of inner cavity, and four support legs are evenly distributed on the bottom of agitator tank,Four sectors are scraped bottom plate and are evenly distributed on drive rod.
Preferably, in the inside of inner cavity, the loop pipe is revolved from the bottom to top for the heater strip contorted winding from the bottom to topTurn shape around being located between heater strip in seam, the spacing between the heater strip and loop pipe is not less than two centimetres.
Preferably, the bottom of the drive rod extends downward into two centimeters of the top of the inner bottom wall of agitator tank.
(3) advantageous effect
Compared with prior art, the utility model provides a kind of cow's serum complement elimination constant temperature mixing stirring device,Has following advantageous effect：
1, the cow's serum complement, which is eliminated, uses constant temperature mixing stirring device, and cow's serum is imported agitator tank by inlet connectorInside, then by driving stirring motor to drive drive rod rotation, the rotation of drive rod that paddle and sector will be driven to scrape bottom plateRotation, wherein the fan-shaped cow's serum deposition scraped bottom plate and can prevent from generating because of stirring rotation, which is effectively by oxSerum stirs evenly, the effective complement rejected in cow's serum.
2, the cow's serum complement, which is eliminated, uses constant temperature mixing stirring device, inside agitator tank when the temperature is excessively high, passes through cycleCooling liquid is recycled in pump driving circuit pipe, the processing in loop pipe after cooling liquid absorption heat in process condenser,The agitator tank that enters again absorbs heat, and work repeatedly effectively reduces the temperature inside agitator tank, stirs simultaneouslyWhen temperature inside tank is too low, by the heating of heater strip, to improve the temperature of agitator tank, which is effectively guaranteed stirringTank internal temperature it is constant always, effectively prevent from destroying the nutrition of serum, cell because temperature is excessively high or too lowAdherent, proliferation and the function of maintaining.
Description of the drawings
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is the utility model structure top view.
In figure：1 agitator tank, 2 support legs, 3 mounting rings, 4 stirring motors, 5 drive rods, 6 paddles, 7 sectors scrape bottom plate, 8Check ring, 9 inlet connectors, 10 temperature probes, 11 air pressure valves, 12 discharge nozzles, 13 discharging control connectors, 14 probe tubes, 15 takeSample control joint, 16 inner cavities, 17 heater strips, 18 loop pipes, 19 circulating pumps, 20 condensers, 21 connecting tubes.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility modelClearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than wholeEmbodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative workThe every other embodiment obtained, shall fall within the protection scope of the present invention.
Please refer to Fig.1-2, a kind of cow's serum complement, which is eliminated, uses constant temperature mixing stirring device, including agitator tank 1, agitator tank 1Bottom be fixedly mounted there are four support leg 2, four support legs 2 are evenly distributed on the bottom of agitator tank 1,1 top of agitator tankMounting ring 3 is installed at center, the convenient fixation to stirring motor 4 of mounting ring 3, the top of mounting ring 3 is installed withStirring motor 4, stirring motor 4 are that paddle 6 and sector scrape bottom plate 7 and provide power, and peace is fixed in the bottom of 4 output shaft of stirring motorEquipped with the drive rod 5 through agitator tank 1, the bottom of drive rod 5 extends downward into two centimetres of the top of the inner bottom wall of agitator tank 1Place, the outside of drive rod 5 are installed with the paddle 6 being evenly distributed, and paddle 6 makes serum stir more uniform, drivingThere are four sectors to scrape bottom plate 7 for the bottom fixed installation of bar 5, and sector is scraped bottom plate 7 and can be prevented because of the ox that stirring rotates and generatesSerum deposits, and four sectors scrape bottom plate 7 and be evenly distributed on drive rod 5, the outside of drive rod 5 and positioned at the interior of agitator tank 1Check ring 8 is installed on roof, check ring 8 prevents serum from overflowing the inside of agitator tank 1 upwards along drive rod 5, stirsThe top of tank 1 and the left side for being located at stirring motor 4, which are interted, is provided with inlet connector 9, and inlet connector 9 facilitates the importing of serum,The top of agitator tank 1 and the right side for being located at stirring motor 4, which are interted, is provided with temperature probe 10, and temperature probe 10 detects temperature,Convenient to control the temperature inside agitator tank 1, one end of 10 bottom of temperature probe is run through and extends to the interior of agitator tank 1Portion, the top of agitator tank 1 and the back side for being located at stirring motor 4, which are interted, is provided with air pressure valve 11, and gas can be discharged in air pressure valve 11Body can also control the pressure inside agitator tank 1, and the bottom of agitator tank 1 is interspersed to be provided with discharge nozzle 12, the right end of discharge nozzle 12It is installed with discharging control connector 13, the lower section in 1 left side of agitator tank is interspersed to be provided with probe tube 14, convenient pair of probe tube 14The left end of the sample detection of serum and observation, probe tube 14 is installed with sampling control joint 15, and the inside of agitator tank 1 opens upThere are inner cavity 16, probe tube 14 to be located at the lower section of inner cavity 16, the inside of inner cavity 16 is arranged with heater strip 17, and heater strip 17 improves stirringTemperature inside tank 1, for the contorted winding from the bottom to top of heater strip 17 in the inside of inner cavity 16, the inside of inner cavity 16 is arranged with circuitPipe 18, for loop pipe 18 to the cooling liquid of its inside into circuit, loop pipe 18 is contorted from the bottom to top around being located at heater strip 17Between in seam, spacing between heater strip 17 and loop pipe 18 is not less than two centimetres, and agitator tank 1 is run through in the one end at 18 top of loop pipeAnd be bent downwardly and be connected to the fixation of circulating pump 19, one end of 18 bottom of loop pipe run through agitator tank 1 and be bent upwards with it is coldCondenser 20 fixes connection, and the right side of agitator tank 1 is installed with circulating pump 19, and circulating pump 19 provides power for cooling liquid, stirsIt mixes the right side of tank 1 and is installed with condenser 20 positioned at the lower section of circulating pump 19, condenser 20 is to the drop after absorbing heatGeothermal liquid cools down, and connecting tube 21 is fixedly connected between condenser 20 and circulating pump 19, and cow's serum is passed through inlet connector 9The inside of agitator tank 1 is imported, then by driving stirring motor 4 that drive rod 5 is driven to rotate, the rotation of drive rod 5, which will drive, stirsLeaf 6 and the fan-shaped rotation for scraping bottom plate 7 are mixed, wherein fan-shaped bottom plate 7 of scraping can prevent the cow's serum generated because of stirring rotation heavyProduct, which effectively stir evenly cow's serum, the effective complement rejected in cow's serum, also, the temperature inside agitator tank 1It when spending high, is recycled by the liquid that cools down in 19 driving circuit pipe 18 of circulating pump, cool down liquid absorption heat in loop pipe 18In the processing by condenser 20 after amount, the agitator tank 1 that enters again absorbs heat, and work repeatedly effectively reducesTemperature inside agitator tank 1, while when temperature inside agitator tank 1 is too low, by the heating of heater strip 17, to improve agitator tank1 temperature, which are effectively guaranteed the constant always of 1 internal temperature of agitator tank, effectively prevent because temperature it is excessively high orPerson is too low and destroys the adherent nutrition of serum, cell, proliferation and the function of maintaining.
In conclusion the cow's serum complement, which is eliminated, uses constant temperature mixing stirring device, cow's serum is imported by inlet connector 9The inside of agitator tank 1, then by driving stirring motor 4 that drive rod 5 is driven to rotate, the rotation of drive rod 5 will drive paddle 6The rotation of bottom plate 7 is scraped with sector, wherein fan-shaped bottom plate 7 of scraping can prevent the cow's serum deposition generated because of stirring rotation, it shouldMode effectively stirs evenly cow's serum, the effective complement rejected in cow's serum.
Also, the cow's serum complement, which is eliminated, uses constant temperature mixing stirring device, inside agitator tank 1 when the temperature is excessively high, passes throughThe liquid that cools down in 19 driving circuit pipe 18 of circulating pump is recycled, by condensing after the liquid absorption heat that cools down in loop pipe 18The processing of device 20, the agitator tank 1 that enters again absorb heat, and work repeatedly effectively reduces inside agitator tank 1Temperature, while when temperature inside agitator tank 1 is too low, by the heating of heater strip 17, to improve the temperature of agitator tank 1, the partyFormula is effectively guaranteed the constant always of 1 internal temperature of agitator tank, effectively prevents from destroying because temperature is excessively high or too lowThe function that the nutrition of serum, cell are adherent, are proliferated and maintain.
It should be noted that herein, relational terms such as first and second and the like are used merely to a realityBody or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operationIn any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended toNon-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including thoseElement, but also include other elements that are not explicitly listed, or further include for this process, method, article or equipmentIntrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded thatThere is also other identical elements in process, method, article or equipment including the element.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,It is appreciated that can these embodiments be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaiiedChange, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.