Utility model content
The purpose of this utility model is that there are the above problem for existing technology, it is proposed that during a kind of dog c reactive proteinBetween resolved fluorometric immunochromatographytest test kit, be used for realization the quick detection of dog c reactive protein.
The purpose of this utility model can be realized by following technical proposal：
Dog c reactive protein time-resolved fluoroimmunoassay chromatographs detection kit, including box body, and buffer solution is embedded with box bodyBottle, suction nozzle and test card, one end of test card is handle end, and the other end of test card is information terminal, and information terminal is equipped with wordLayer, test card include cover board, test strips and bottom plate, and test strips are fixed between cover board and bottom plate, and the outer wall of cover board, which is equipped with, to be knownThe barcode scanning layer of other detection object, test strips include connected sample pad, labeling pad, detecting pad and the water absorption pad of overlap joint；LabelThe anti-dog c reactive protein antibody one of mouse of time-resolved fluorescence microballoon mark is coated with pad, be installed with detecting pad detection line andNature controlling line, nature controlling line are adjacent to water absorption pad, and detection line is coated with the anti-dog c reactive protein antibody two of mouse, and nature controlling line is coated with goat-antiMouse secondary antibody；Cover board offers loading slot and detect tank, and the loading slot of cover board towards sample pad and surrounds out sample-adding portion, and sample-adding portion is leaned onIt is bordering on handle end；The detect tank of cover board is towards detecting pad and surrounds out test section, and test section is close to information terminal, detection line and matterControl line is located in test section.
In above-mentioned dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit, sample pad is glass fibre,Filter paper or hemofiltration film；Labeling pad is glass fibre, filter paper or polyester film；Detecting pad is nitrocellulose filter or acetic acidCellulose membrane；Water absorption pad is blotting paper.In above-mentioned dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit,Either Quick Response Code barcode scanning layer is cohesive or is printed between loading slot and detect tank for bar code for barcode scanning layer.
In above-mentioned dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit, the outer wall of bottom plate offersSliding slot, the depth of sliding slot are less than the thickness of bottom plate, and sliding slot extends to handle end along the central axes of bottom plate from information terminal.
In above-mentioned dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit, one end of sampling rod is cottonCaput, the other end of sampling rod is fluid suction head.
Compared with prior art, the technical program has the advantage that：
1st, reagent cartridge configuration is simple, easy to operation, during detection, sample liquid is added drop-wise to after being reacted 15 minutes on test card, justIt can be detected as a result, detection time is fast, without relying on complicated detecting instrument, be not only restricted to detection occasion；Barcode scanning layerSetting improve the speed of quantitative detection, it is not necessary to setting detection object is artificially gone to fluorescence detector, saves operationTime, it is therefore prevented that the situation generation of error and standard curve download mistake is manually set, it is ensured that the accuracy of detection and analysis, it is realNow quickly quantitative detection.
2nd, sliding slot plays position-limiting action, defines the insertion depth of test card, in order to operate, during quantitative analysis, in cunningGroove it is spacing under, handle end is in outside socket, and sample-adding portion is in socket, so that it is outer to avoid in quantitative analysis sample-adding portion from being subject toPollute on boundary, it is ensured that the accuracy of detection.
Brief description of the drawings
Fig. 1 is the structural representation of the dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit of the utility modelFigure.
Fig. 2 is the test card of the dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit of the utility modelFront schematic view.
Fig. 3 is the test card of the dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit of the utility modelSchematic rear view.
Fig. 4 is the test card of the dog c reactive protein time-resolved fluoroimmunoassay chromatography detection kit of the utility modelSectional view.
In figure, 1, box body；2nd, test card；3rd, cover board；4th, test strips；5th, bottom plate；6th, sample pad；7th, labeling pad；8th, examineSurvey pad；9th, water absorption pad；10th, detection line；11st, nature controlling line；12nd, loading slot；13rd, detect tank；14th, sample-adding portion；15th, test section；16、Character layer；17th, barcode scanning layer；18th, handle end；19th, information terminal；20th, buffer solution bottle；21st, suction nozzle；22nd, sampling rod；23rd, sampling bottle；24th, sliding slot；25th, swab stick head；26th, fluid suction head.
Embodiment
It is specific embodiment of the utility model and with reference to attached drawing below, the technical solution of the utility model is made furtherDescription, but the utility model is not limited to these embodiments.
As shown in Figures 1 to 4, the utility model provides a kind of dog c reactive protein time-resolved fluoroimmunoassay chromatography detectionKit, for the Quantitative detection of dog c reactive protein, including box body 1, is embedded with one bottle of buffer solution bottle 20, one in box body 1Branch suction nozzle 21 and one piece of test card 2, box body 1 separate out the card slot of multiple and different shapes, buffer solution bottle 20, suction nozzle 21 and test card2 are embedded in the corresponding card slot of shape therewith respectively, to avoid mutual collision.A operation instruction is also placed with box body 1Book.
Sample is added into vibrated in buffer solution bottle 20 after form sample liquid, suction nozzle 21 be used for Aspirate supernatant and by itsIt is added drop-wise on test card 2.Selectively, a sampling rod 22 and a sampling bottle 23 are also embedded with box body 1, sampling rod 22 carriesTake sample and transfer the sample into sampling bottle 23, buffer solution is then added into sampling bottle 23 vibrates to form sample liquid, then usesSuction nozzle 21 draws the supernatant of sample liquid and is added dropwise to test card 2 and is detected.
One end of test card 2 is handle end 18, and when operation holds pommel 18 of stopping, and the other end of test card 2 is information terminal19, information terminal 19 is equipped with the character layer 16 of display detection object, and character layer 16 has the name information on detection object, textWord layer 16 confirms to be used for operator, and operator confirms whether the test card 2 is suitable for detection dog C protein by character layer 16,Avoid malfunctioning.
Test card 2 includes cover board 3, test strips 4 and bottom plate 5, and test strips 4 are fixed between cover board 3 and bottom plate 5, test paperBar 4 is bonded in the inner wall of bottom plate 5, and the lid of cover board 3 is located in test strips 4 and connects with the engaging of bottom plate 5.The outer wall of cover board 3 is equipped withThe barcode scanning layer 17 of recognition detection object, barcode scanning layer 17 have the coding information on detection object.
During quantitative analysis, test card 2 coordinates fluorescence detector (existing instrument) to use, and sample liquid is added drop-wise in test strips 4,Fluorescence signal is detected with fluorescence detector after reacting 15 minutes, the utility model focuses on kit, and fluorescence detector is notMake specific expansion, test card 2 is inserted into the concrete numerical value information that dog c reactive protein just can be exported in fluorescence detector.
It is noted that barcode scanning layer 17 is identified detection object for fluorescence detector, fluorescence detector scanningAfter barcode scanning layer 17 recognizes detection object, the standard curve for obtaining dog c reactive protein is downloaded, fluorescence detector detects that fluorescence is believedThe dog c reactive protein concentration in sample is calculated according to dog c reactive protein standard curve after number.The setting of barcode scanning layer 17 improvesThe speed of quantitative detection, it is not necessary to setting detection object is artificially gone to fluorescence detector, saves the operating time, it is therefore prevented thatThe situation generation of error and dog c reactive protein standard curve download mistake is manually set, it is ensured that the accuracy of detection and analysis.
Specifically, test strips 4 include connected sample pad 6, labeling pad 7, detecting pad 8 and the water absorption pad 9 of overlap joint, labelPad 7 is between sample pad 6 and detecting pad 8, and detecting pad 8 is between labeling pad 7 and water absorption pad 9.
The anti-dog c reactive protein antibody one of mouse of time-resolved fluorescence microballoon mark, detecting pad 8 are coated with labeling pad 7On be installed with detection line 10 and nature controlling line 11, detection line 10 is coated with the anti-dog c reactive protein antibody two of mouse, and nature controlling line 11 is coated withSheep anti mouse secondary antibody；Detection line 10 and nature controlling line 11 are parallel, and nature controlling line 11 is adjacent to water absorption pad 9.The anti-dog c reactive protein antibody of mouseOne and the anti-dog c reactive protein antibody two of mouse be two different epitopes the anti-dog c reactive protein antibody of mouse, i.e., using double-antibody sandwichMethod.
Cover board 3 offers loading slot 12 and detect tank 13, and the loading slot 12 of cover board 3 towards sample pad 6 and surrounds out sample-addingPortion 14, sample-adding portion 14 is close to handle end 18；The detect tank 13 of cover board 3 is towards detecting pad 8 and surrounds out test section 15, test section15 are located in test section 15 close to information terminal 19, detection line 10 and nature controlling line 11.In addition, the side of detect tank 13 is equipped with detectionLine 10 marks T and nature controlling line 11 marks C, and detection line 10 marks the detection line 10 of T direct detections pad 8, and nature controlling line 11 marks C to be directed towardThe nature controlling line 11 of detecting pad 8, easy to qualitatively judge, qualitative judgement can be visually observed directly using laser irradiating and detecting portion 15.
Further, the cell wall of loading slot 12 slopes inwardly, and the top edge size of loading slot 12 is more than its lower edge size,Sample liquid can be formed and converged.The cell wall of detect tank 13 slopes inwardly, and the top edge size of detect tank 13 is more than its lower edge size,To form the laser entry portal of big opening.Character layer 16 bond or be printed on cover board 3 upper end edge and detect tank 13 itBetween.
The material that bottom plate 5 and cover board 3 are PVC boards or other hard do not absorb water, sample pad 6 for glass fibre, filter paper orHemofiltration film；Labeling pad 7 is glass fibre, filter paper or polyester film, can be with when sample pad 6 is identical with the material of labeling pad 7Combine；Detecting pad 8 is nitrocellulose filter or cellulose acetate film, preferably nitrocellulose filter；Water absorption pad 9 isBlotting paper.
Selectively, for bar code, either Quick Response Code barcode scanning layer 17 bonds or is printed on loading slot 12 to barcode scanning layer 17Between detect tank 13, to separate the reading of the collection of fluorescence signal and coding information.
Selectively, the outer wall of bottom plate 5 offers sliding slot 24, and the depth of sliding slot 24 is less than the thickness of bottom plate 5, and sliding slot 24 is notThrough the inner wall of bottom plate 5, the bottom surface of test strips 4 will not be revealed in sliding slot 24, so as to avoid test strips 4 from being contaminated.Sliding slot24 length is less than the length of bottom plate 5, and sliding slot 24 extends to handle end along the inward flange of the central axes of bottom plate 5 from information terminal 19There is spacing between the both ends of 18 inward flange, sliding slot 24 and bottom plate 5.
Sliding slot 24 plays position-limiting action when test card 2 is inserted into fluorescence detector, defines the insertion depth of test card 2, withIt is easy to operation.During quantitative analysis, hold live test card 2 handle end 18, by information terminal 19 towards fluorescence detector socket simultaneouslyInsertion, sliding slot 24 it is spacing under, handle end 18 is in outside socket, and sample-adding portion 14 is in socket, so as to avoid quantitatively dividingSample-adding portion 14 is subject to outside contamination during analysis, it is ensured that the accuracy of detection.
Selectively, one end of sampling rod 22 is swab stick first 25, and the other end of sampling rod 22 is fluid suction head 26.Sampling rod 22Solid and liquid can be extracted at the same time, and swab stick first 25 is used to extract solid fraction sample, and fluid suction head 26 is used for imbibition, easy to operation.
The quantitative testing principle of dog c reactive protein is：Supernatant is taken after sample to be tested is mixed with buffer solution, and by supernatantLiquid after loading slot 12 is added drop-wise in the sample-adding portion 14 of test card 2, dog c reactive protein in sample with solution from sample pad 6 veryFast chromatography arrives labeling pad 7, and dog c reactive protein and the anti-dog C of mouse that time-resolved fluorescence microballoon in labeling pad 7 marks are anti-at this timeProtein antibodies one are answered to form fluorescent microsphere-mouse anti-dog c reactive protein antibody one-dog c reactive protein compound, the compound is with moltenLiquid layer is analysed onto detecting pad 8, is combined when reaching detection line 10 (T lines) with the anti-dog c reactive protein antibody two of the mouse at this, is formed glimmeringLight microballoon-mouse one-dog of anti-dog c reactive protein antibody c reactive protein-anti-two compound of dog c reactive protein antibody of mouse is simultaneously fixedIn T lines, and first the excessive anti-dog c reactive protein antibody of fluorescent microsphere-mouse continues chromatography and arrives nature controlling line 11 (C lines), and hereinSheep anti mouse secondary antibody formed fluorescent microsphere-mouse anti-dog c reactive protein antibody one-sheep anti mouse secondary antibody compound.After chromatographing 15 minutes,With the test section 15 of exciting light irradiating and detecting pad 8, it will detect that T lines, C lines have fluorescence, according to standard curve and fluorescent valueConcentration to be measured can be calculated in ratio.
The specific embodiments described herein are merely examples of the spirit of the present invention.The utility model instituteDescribed specific embodiment can be done various modifications or additions or using similar by belonging to those skilled in the artMode substitute, but without departing from the spirit of the present application or beyond the scope of the appended claims.
Although box body 1 is used more herein；Test card 2；Cover board 3；Test strips 4；Bottom plate 5；Sample pad 6；LabelPad 7；Detecting pad 8；Water absorption pad 9；Detection line 10；Nature controlling line 11；Loading slot 12；Detect tank 13；Sample-adding portion 14；Test section 15；TextWord layer 16；Barcode scanning layer 17；Handle end 18；Information terminal 19；Buffer solution bottle 20；Suction nozzle 21；Sampling rod 22；Sampling bottle 23；Sliding slot 24；Swab stick first 25；The grade term of fluid suction head 26, but it does not preclude the possibility of using other terms.The use of these items is only forMore easily describe and explain the essence of the utility model；Being construed as any one of the additional limitations is all and this practicalityWhat new spirit was disagreed.