A kind of sodium ion biochemical analysis dry tabletTechnical field
This utility model relates to vim and vigour biochemical sensor technologies field, particularly relates to a kind of sodium ion biochemical analysis dry tablet.
Background technology
Human body fluid electrolyte concentration is the important evidence of Clinical Laboratory, treatment assessment and Index for diagnosis.And as the dominant cation of the outer liquid of human body cell, sodium ion is to judge one of important indicator that human electrolyte balances.Sodium ions content equipment in human body fluid of measuring conventional at present is the wet type biochemical analysis detector relying on ion selective electrode principle.
There is complicated operation, operation and maintenance cost height, low-response, the shortcoming such as not portable in traditional wet type biochemical analysis detection.Novel electrolyte detection method dry type detection, has and measures the features such as accurate, with low cost, simple to operate, can be widely used in multiple environment.
Chinese patent CN101852761A discloses a kind of all-solid sodium ion selective electrode and preparation method thereof, but needs when the ion selective electrode in patent is tested to use with the pairing of external reference electrode, using method complexity.And existing solid ionic selects, in electrode, there is response signal instability, the problem that response time is longer.
Summary of the invention
For existing sodium selective electrode Problems existing, this utility model provides a kind of sodium ion biochemical analysis dry tablet, and its certainty of measurement is high, long-time stability are good, and response time is short, and simple in construction, volume are little, preparation technology is simple, and production cost is low, is suitable for producing in enormous quantities.
This utility model sodium ion biochemical analysis dry tablet includes supporting layer, insulating barrier, salt bridge, electrode, reference layer and sodium ion selective membrane, salt bridge is sealed in insulating barrier, insulating barrier is provided with gas port and inlet, supporting layer and insulating barrier are laminated structure, electrode is metal/metal salt thin electrode, electrode includes metal level and metal salt, and electrode adopts the mode of chemical plating, Vacuum Deposition or plating to be attached on substrate, and sodium ion selective membrane is attached on dried reference layer.
This utility model dry tablet supporting layer plays protection, supports sodium selective electrode effect, and insulating barrier plays bonding electrode, seals salt bridge effect, and salt bridge plays connection electrode effect；Salt bridge is sealed in insulating barrier, and insulating barrier has gas port and inlet, can improve the flow rate of reference liquid and test fluid during test, shortens the testing time；Supporting layer, insulating barrier are selected from polypropylene, polrvinyl chloride, polyethylene, polyester non-conductive polymer material, and salt bridge is filter paper material.
Electrode.This utility model electrode includes metal level and metal salt, and metal salt forms phase boundary potential with metal level, has stable electromotive force response.
Electrode metal layer is attached to non-conductive polymer surface, and for improving the stability of electrode, shortening response time, this utility model can adopt the mode of vacuum coating, chemical plating or plating to prepare metal level.Metal salt can adopt the method for chemical oxidation to prepare, and uses iron chloride or potassium permanganate solution that metal level is aoxidized.
Reference layer.Reference layer includes: binding agent, buffer agent, slaine, surfactant.Reference layer is attached directly on electrode metal salt deposit by the mode of lamination or coating.
For guaranteeing that sodium ion biochemical analysis dry tablet has good measuring accuracy and long-time stability, reference layer pH ranges for 5 ~ 8.5, simultaneously for ensureing the bond strength of reference layer and sodium ion selective membrane, prevent sodium ion biochemical analysis dry tablet response signal accuracy and long-time stability from reducing, reference layer water content can not be too high or too low, and reference layer optimum moisture content is 30% ~ 40%.
Sodium ion selective membrane.Sodium ion selective membrane includes high polymer binder, ionophore, carrier solvent, anion agent interfering and surfactant.
Being different from the sodium selective electrode of existing patent, this utility model sodium ion biochemical analysis dry tablet has the advantage that (1) need not use with the pairing of outer reference electrode, and in dry tablet, two panels sodium selective electrode is identical；(2) eliminate liquid electrolyte layer, adopt structure of whole solid state, convenient and swift, need not activate for a long time during use；(3) reference layer pH5 ~ 8.5, water content 30% ~ 40%, improve the certainty of measurement of dry tablet；(4) use surface uniform metal/metal salt thin electrode, be greatly shortened dry tablet response time, within 7s can equilibrium establishment, form stable response electromotive force；(5) simple in construction, volume are little, are sized to 10mm × 10mm, it is easy to integrated, can be developed into superminiature biochemical analysis dry tablet, and preparation technology is simple, is suitable to large-scale industrial production.
Accompanying drawing explanation
Fig. 1 is sodium ion biochemical analysis dry tablet schematic diagram.
Fig. 2 is sodium selective electrode (electrode, reference layer, sodium ion selective membrane) schematic diagram.
Fig. 3 is sodium ion biochemical analysis dry tablet testing standard liquid response potential curve.
Fig. 4 is sodium ion biochemical analysis dry tablet characteristic curve.
Detailed description of the invention
In conjunction with the drawings and specific embodiments, this utility model is described in detail.
As it is shown in figure 1, sodium ion biochemical analysis dry tablet includes: supporting layer 1, sodium selective electrode 2, insulating barrier 3, salt bridge 4.
As in figure 2 it is shown, sodium selective electrode includes: silver layer 21, silver chloride layer 22, reference layer 23, sodium ion selective membrane 24.
Silver layer 21 is attached to polyethylene terephthalate surface, the front 2/3 section of oxidation of silver layer 21 obtains silver chloride layer 22, reference layer solution is attached directly on silver chloride layer 22, sodium ion selective membrane is attached on dry reference layer 23, two panels sodium selective electrode 2 is alignd with inlet on insulating barrier 3, and insulating barrier 3 aligns with supporting layer 1 bonding composition dry tablet.
Sodium ion biochemical analysis dry tablet long-term storage conditions is :-18 ~-10 DEG C.
Sodium ion biochemical analysis dry tablet using method, needs when taking out from low temperature environment to place at normal temperatures and uses after 1h, keeps test temperature 37 ± 0.1 DEG C during test.During test, dry tablet is inserted in test trough, two lateral opening dropping reference liquid and test fluid, required sample 5 ~ 10 μ L respectively.
In order to better illustrate advantage of the present utility model, this utility model provides 2 groups of experimental datas, specific as follows.
Experiment 1, with sodium ion biochemical analysis dry tablet testing standard liquid, the curve of response electromotive force and testing time is as shown in Figure 3.
Experiment 2, calibrates sodium ion biochemical analysis dry tablet.By known Na ion concentration titer (Na ion concentration 95mmol/L and 220mmol/L), test sodium ion biochemical analysis dry tablet response electromotive force, obtain electromotive force response corrections curve.Correction equation is: Y=61.42X+54.89, and wherein Y represents response electromotive force, and X represents that logC, C are sodium ion in liquor concentration.Characteristic curve is as shown in Figure 4.
The above only for technology of the present utility model design and feature are described, its object is to allow person skilled in the art will appreciate that content of the present utility model and to implement according to this, can not limit protection domain of the present utility model with this.All modifications done according to the spirit of this utility model main technical schemes, all should be encompassed within protection domain of the present utility model.