A kind of Full Automatic Liquid basal cell length of schooling sheet coloring systemTechnical field
The utility model relates to a kind of film-making coloring system, particularly relates to a kind of Full Automatic Liquid basal cell length of schooling sheet coloring system.
Background technology
Detect in medical science and in biological study, often need by carrying out film-making and dyeing to cell sample, to observe better.Traditional film-making dyeing course all adopts manual operations, but affect correct diagnosis because manual smear also exists the disturbing factor such as a large amount of cell accumulations and red blood cell, mucus, impurity, there will be the false negative rate of 2% ~ 50%, and easily make diagnostic personnel eye work fatigue, and waste time and energy, the problems such as efficiency is low.In recent years, the application that liquid basal cell learns a skill makes the film-making of cell and staining technique have new breakthrough, it is that cast-off cells are kept in cell-preservation liquid by one that liquid basal cell learns a skill, effectively can dispel the interference diagnosis factors such as red blood cell, mucus, impurity and cell mass be disperseed dissociation, remarkable change pathocytology slice-making quality, eucaryotic cell structure and clear background, be beneficial to antidiastole, significantly reduces false negative rate.
Pelleter in the market has film (suction) formula pelleter, common centrifugal pelleter and natural sedimentation pelleter, wherein there is following shortcoming or deficiency in film suction pelleter and common centrifugal pelleter, cause its diagnostic accuracy of production effect de-stabilising effect: 1, no specimen pretreatment function, easily affect and reduce slice-making quality by undissolved mucus, impurity and too much inflammatory cell etc., the interpretation of impact knot; 2, sample process ability is little, and film (suction) formula pelleter is disposable can only process 1 increment originally, and common centrifugal pelleter single treatment 6 ~ 8 increment originally; 3, after sectioning cells for preventing cell from falling sheet, usually adopt and to dry or heating plate carries out heat fixation, easily cause cytomorphosis; 4, membrane type pelleter easily makes the low confluent monolayer cells (cellule) of most diagnostic significance because the effect of negative pressure is lost through filtering membrane, has a strong impact on diagnostic result accuracy; 5, before, during and after sample film-making, whole process needs operating personnel's synchronous synergetic to operate, and operates cumbersome efficiency low; 6, dyeing course must be completed by manual operation, easily occurs that human factor affects dyeing quality.
Although natural subsidence formula liquid-based cell sample manufacturing overflow dyeing machine can realize film-making and dyeing robotization, but still there is the following deficiency in it: 1, its principle adopting the effect of cell self gravitation to be deposited on microslide carries out film-making, because of cell lighter weight, therefore film-making speed is slow, and efficiency is not high.In addition, natural subsidence attaches tight to the cell of slide and slide, easily in follow-up operation, cause cell detachment, lose efficient diagnosis composition; 2, the pretreatment function of this pelleter no specimen, cell purification process comprises twice gradient centrifugation, mixing, sample transfer etc., and need manual operation to complete in conjunction with other equipment, complicated operation efficiency is low; 3, the abnormal cell of high liquid level cannot realize preferentially catching, and affects diagnostic result accuracy to a certain extent.
The patent No. is describe a kind of Full Automatic Liquid basal cell length of schooling sheet coloring system in the patent documentation of CN201020525550.2, which describes a kind of centrifugal smear liquid-based cell sheet processing dyeing machine.This utility model achieves dyeing, film-making integration automatic operation, but still has the following disadvantages: (1) wherein sample directly carries out film-making dyeing by leaving standstill, and makes the cell of catching be vulnerable to impurity interference, thus the accuracy of impact diagnosis.(2) liquid-feeding tube of described photo making cup cannot be separated with plaster mechanism, the cleaning of inconvenient liquid-feeding tube, replacing.(3) be only provided with two and move liquid arm, complete the multinomial work such as transfer, dyeing, waste discharge, easily cause cross pollution.(4) without refrigerating plant, because motor runs up in centrifugal process, easily cause temperature too high, the normal operation of impact machinery.
Utility model content
The utility model provides a kind of Full Automatic Liquid basal cell length of schooling sheet coloring system, its object is to realize full-automatic and standardization sample purification process, film-making and dyeing, effectively can diagnose composition by purifying cells, increase cell quantity, reduce impurity interference, photo making cup for convenience detach, prevents the technical matterss such as cross pollution, improves diagnostic result accuracy.
In order to solve the problems of the technologies described above, the technical solution of the utility model is as follows:
A kind of Full Automatic Liquid basal cell length of schooling sheet coloring system, comprise cabinet, the hood that can cover on this cabinet, the control system be located in this cabinet, the sample disk be located on this cabinet, film-making dish and the pipetting device for shifting sample, described sample disk is provided with sample arm, rotatingly on this sample arm be linked with several hanging baskets, this hanging basket is with described sample disk center of circle layout in a center of symmetry, each hanging basket is provided with some sample cups, and this sample cup can set up sample cup; Described film-making dish is provided with circumferentially uniform film-making cup, and this film-making cup can set up photo making cup, and this photo making cup comprises plaster mechanism and dismountable liquid-feeding tube being connected to this plaster mechanism; Described sample disk and film-making dish are horizontal centrifugal rotating disk; Described cabinet is also provided with the filling mechanism for adding reagent, for stir sample rabbling mechanism, for dripping the dyeing mechanism of coloring agent and the waste liquid absorbing mechanism for absorbing waste liquid.
Further, the plaster mechanism of described photo making cup is the housing offering vertical paster groove, and its one side offers and adds liquid bath, and this adds liquid bath and communicates with vertical paster, and this adds liquid bath outer edge and is provided with position limiting clamp; Described liquid-feeding tube is L shape pipe, and its one end can be inserted in and add in liquid bath, and the limited block be provided with by outside surface is coordinated with described position limiting clamp and clamps, and the axle center of its other end straight up.
Further, described pipetting device comprise can stretch up and down pipetting device pole, rotate along this pipetting device pole move liquid arm, be located at this and move the liquid-transfering needle below liquid arm and the first service sink for cleaning liquid-transfering needle, described liquid-transfering needle can turn to directly over sample cup, photo making cup and the first service sink with moving liquid arm.
Further, described rabbling mechanism comprise the rabbling mechanism pole that can stretch up and down, the mixing arm rotated along this rabbling mechanism pole, be located at below this mixing arm can the mixing needle of high-speed rotation and the second service sink for cleaning mixing needle, described mixing needle can turn to directly over sample cup and the second service sink with mixing arm.
Further, described waste liquid absorbing mechanism comprise the waste liquid absorbing mechanism pole that can stretch up and down, the waste liquid arm rotated along this waste liquid absorbing mechanism pole, be located at below this waste liquid arm waste liquid pin and the 3rd service sink for cleaning waste liquid pin, this waste liquid pin can turn to directly over photo making cup and the 3rd service sink with waste liquid arm.
Further, described filling mechanism comprises the filling mechanism pole that can stretch up and down, the liquid adding arm rotated along this filling mechanism pole, be located at liquid feeding pin below this liquid adding arm and aspirating needle and the 4th service sink for cleaning this liquid feeding pin and aspirating needle, this liquid feeding pin is mutually close with aspirating needle, and the two can turn to directly over sample cup and the 4th service sink with liquid adding arm.
Further, described liquid feeding pin and aspirating needle have many groups, and with the sample cup one_to_one corresponding on same described hanging basket.
Further, described dyeing mechanism comprise the dyeing mechanism pole that can stretch up and down, the dyeing arm rotated along this dyeing mechanism pole, be located at below this dyeing arm entry needle, this entry needle can turn to directly over photo making cup with the arm that dyes.
Further, refrigerating plant is provided with in the cabinet below described sample disk and film-making dish.
Further, several film-making cups that described film-making dish is provided with evenly are arranged into inside and outside two circles along this film-making disk center.
The utility model has the advantage of: integrate cytological samples purification process, film-making, dye, automatic intelligent operates, and processes multiple sample, time saving and energy saving, and diagnosis effect is good.(1) wherein sample carries out gradient centrifugation by sample disk, can purifying cells, makes cell more cleaner, does not have impurity disturb, also can increase cancer cell and catch quantity, raising diagnostic accuracy.(2) liquid-feeding tube of described photo making cup and plaster mechanism detachable, facilitate the cleaning of liquid-feeding tube, replacing.(3) be provided with pipetting device, waste liquid absorbing mechanism, filling mechanism and dyeing mechanism, separately each step operated, stop cross pollution.(4) described sample disk adopts the structure of basket-hanging, and time centrifugal, the level of force of hanging basket is outside, hanging basket is presented horizontal, at the bottom of the cell in sample cup can be made like this to sink to glass; At the end of centrifugal, bottom hanging basket, deadweight, makes described sample cup upwards erect downwards, places separator like this and again obscures, also make liquid feeding pin vertically can pass in and out sample cup work with aspirating needle.(5) be provided with refrigerating plant in the cabinet below described sample disk and film-making dish, to cool the motor run up in cabinet in centrifugal process, thus machinery normally, stably operated.(6) add rabbling mechanism, cell dispersed with stirring can be made.(7) be provided with hood, prevent the extraneous pollution produced in film-making dyeing course from entering.
Accompanying drawing explanation
Fig. 1 is the schematic diagram that the utility model overlooks direction.
Fig. 2 is the schematic diagram of the main apparent direction of the utility model.
Fig. 3 is the schematic diagram of sample cup described in the utility model.
Fig. 4 is the schematic diagram of photo making cup described in the utility model.
Embodiment
Embodiment of the present utility model is as follows:
With reference to Fig. 1, a kind of Full Automatic Liquid basal cell length of schooling sheet coloring system, comprise cabinet 1, the hood 2 on this cabinet can be covered at, be located at the control system in this cabinet 1, be located at the sample disk 10 on this cabinet 1, film-making dish 11, pipetting device 12, filling mechanism 15, rabbling mechanism 16, waste liquid absorbing mechanism 17 and dyeing mechanism 18, described sample disk 10 is provided with sample arm 100, described sample arm 100 is made up of 4 bottom interconnective " Y " type supports, 4 " Y " types are symmetrical along sample disk 10 center of circle, and through hole should be had by " Y " type bracket legs end opens, rotatingly on this sample arm 100 be linked with 4 hanging baskets 101, the latch 1010 that this hanging basket 101 symmetria bilateralis is provided with, this latch 1010 can leave surplus through described through hole in through hole, to realize the rotation of hanging basket 101.Each hanging basket 101 is evenly provided with 6 sample cups 102, this sample cup 102 can set up sample cup 13; Described film-making dish 11 is provided with circumferentially uniform film-making cup 110, and this film-making cup 110 can set up photo making cup 14.Described sample disk 10 and film-making dish 11 are centrifugal turntable, be provided with refrigerating plant, to cool the motor run up in cabinet 1 in centrifugal process, prevent due to the overheated mechanical fault caused in the cabinet 1 below this sample disk 10 and film-making dish 11.
See figures.1.and.2, described pipetting device 12 comprise can stretch up and down pipetting device pole 120, rotate along this pipetting device pole 120 move liquid arm 121, be located at this and move the liquid-transfering needle 122 below liquid arm 121 and the first service sink 123 for cleaning liquid-transfering needle 122, described liquid-transfering needle 122 can be used for absorbing and injection sample, can turn to directly over sample cup 13, photo making cup 14 and the first service sink 123 with moving liquid arm 121.
See figures.1.and.2, described filling mechanism 15 comprises the filling mechanism pole 150 that can stretch up and down, the liquid adding arm 151 rotated along this filling mechanism pole 150, be located at liquid feeding pin 152 below this liquid adding arm 151 and aspirating needle 153 and the 4th service sink 154 for cleaning liquid feeding pin 152 aspirating needle 153, and this liquid feeding pin 152 and aspirating needle 153 can turn to directly over sample cup 13 and the 4th service sink 154 with liquid adding arm 151.Described liquid feeding pin 152 and aspirating needle 153 respectively have 6, two two-phases near composition one group, 6 groups of liquid feeding pins 152 and aspirating needle 153 and 6 sample cup 102 one_to_one corresponding on same described hanging basket 101; Described 4th service sink 154 is 6, with liquid feeding pin 152 described in 6 groups and aspirating needle 153 one_to_one corresponding.Described often group liquid feeding pin 152 and aspirating needle 153 can pass in and out in same sample cup 13 and the 4th service sink 154 jointly.This liquid feeding pin 152 is connected with the reagent bottle of a splendid attire reagent, and this aspirating needle 153 can be used for supernatant collection in a waste liquid pool.
See figures.1.and.2, described rabbling mechanism 16 comprise can stretch up and down rabbling mechanism pole 160, along this rabbling mechanism pole 160 rotate mixing arm 161, be located at below this mixing arm 161 can with the mixing needle 162 of a stirring motor high-speed rotation and the second service sink 163 for cleaning mixing needle 162, described mixing needle 162 can turn to directly over sample cup 13 and the second service sink 163 with mixing arm 161.
See figures.1.and.2, described waste liquid absorbing mechanism 17 comprising the waste liquid absorbing mechanism pole 170 that can stretch up and down, the waste liquid arm 171 rotated along this waste liquid absorbing mechanism pole 170, being located at the waste liquid pin 172 below this waste liquid arm 171 and the 3rd service sink 173 for cleaning waste liquid pin 172, this waste liquid pin 172 can turn to directly over photo making cup 14 and the 3rd service sink 173 with waste liquid arm 171, described waste liquid pin 172 and the 3rd service sink 173 4 groups of having position relationship corresponding, this waste liquid pin 172 can draw the waste liquid in 4 photo making cups 14 simultaneously, and cleans simultaneously.And this waste liquid pin 172 can be used for waste collection in a waste liquid pool.
See figures.1.and.2, described dyeing mechanism 18 comprise can stretch up and down dyeing mechanism pole 180, along pole 180 rotation of this dyeing mechanism dyeing arm 181, be located at below this dyeing arm 181 entry needle 182, this entry needle 182 can turn to directly over photo making cup 14 with dyeing arm 181, described entry needle 182 has 2 groups, can inject in 2 photo making cups 14 respectively.And this entry needle 182 is connected with the coloring agent bottle being loaded with coloring agent.
With reference to Fig. 3, described photo making cup 14 comprises plaster mechanism 140 and dismountable liquid-feeding tube 141 being connected to this plaster mechanism 140, the plaster mechanism 140 of described photo making cup 14 is for offering the housing of vertical paster groove 142, its one side offers and adds liquid bath 143, this adds liquid bath 143 and communicates with vertical paster groove 142, this adds liquid bath 143 outer edge and is provided with position limiting clamp 144, and this position limiting clamp 145 1 side is provided with opening; Described liquid-feeding tube 141 is L shape pipe, and its one end can be inserted in and add in liquid bath 143, and the limited block 146 be provided with by outside surface is coordinated with described position limiting clamp 145 and clamps, and the axle center of its other end straight up.Described limited block 146 and position limiting clamp 145 are divided into along adding just right two groups in liquid bath 143 center, opening direction wherein on two position limiting clamps 145 is contrary, during installation, described two limited blocks 146 are relative with the opening of two position limiting clamp 145 sides respectively, by rotating described liquid-feeding tube 141, make limited block 146 from opening, snap in position limiting clamp 145; During dismounting, rotate backward this liquid-feeding tube 141.
In addition, the number of described " Y " type support and hanging basket 101 can be other suitable number such as 6 or 8; The number of the sample cup 102 on described each hanging basket 101, the liquid feeding pin 152 in filling mechanism 15, aspirating needle 153 and the 4th service sink 154 can be other suitable number such as 2 or 4; Waste liquid pin 172 in described waste liquid absorbing mechanism 17 and the 3rd service sink 173 can be other suitable number such as 1 or 2; Entry needle 182 in described dyeing mechanism 18 can be other suitable number such as 1 or 4.
With reference to Fig. 1, Fig. 2, Fig. 3 and Fig. 4, the step that use the utility model carries out automatic film-making dyeing is as follows:
(1) sample cup 13 being loaded with conserving liquid sample is placed in the sample cup 100 of sample disk 10, photo making cup 14 is positioned in the film-making cup 110 of film-making dish 11.
(2) start computer work function software, all moving component automatic calibration location of system are also cleaned liquid-transfering needle 122, mixing needle 162, waste liquid pin 172, aspirating needle 153.
(3) liquid adding arm 151 goes to above No. 1 hanging basket 101, and 6 liquid feeding pins 152 add specific gravity liquid simultaneously in 6 sample cups 13 on this hanging basket 101, go to the 4th service sink 154 immediately and clean described liquid feeding pin 152 inside and outside wall.By that analogy, in the sample cup 13 on each hanging basket 101, specific gravity liquid is added.
(4) sample disk 10 carries out centrifugal, automatic aligning, make No. 1 hanging basket 101 be positioned at below liquid adding arm 151, the supernatant on No. 1 hanging basket 101 in 6 sample cups 13 removed by 6 aspirating needles 153 simultaneously, and liquid adding arm 151 is transferred to the 4th service sink 154 and cleans described aspirating needle 153 inside and outside wall immediately.By that analogy, the supernatant on each hanging basket 101 in sample cup 13 is removed.
(5) mixing arm 161 and sample disk 10 coordinate rotation, mixing arm 161 is made to be positioned at directly over No. 1 sample cup 13, mixing needle 162 High Rotation Speed stirs sample liquid, make cell or pathogen dispersion mixing in sample, after No. 1 sample has stirred, mixing arm 161 forwards the second service sink 163 position to and carries out 2 repeated washing to mixing needle 162.By that analogy, stir process is carried out to all samples.
(6) liquid arm 121 and sample disk 10 cooperation rotation is moved, making to move liquid arm 121 is positioned at directly over No. 1 sample cup 13, liquid-transfering needle 122 draws 1000ul sample liquid, to move directly over No. 1 photo making cup 14 that liquid arm 121 is transferred to film-making dish 11 and with liquid-transfering needle 122, sample to be injected photo making cup 14, moving liquid arm 121 immediately and forward the first service sink position to and carry out repeating inside and outside cleaning for 2 times to liquid-transfering needle 122.By that analogy, all samples are shifted.When sample size encloses photo making cup 14 quantity less than 1, system automatic symmetrical balance transfer application of sample.
(7) film-making dish 11 high speed horizontal centrifugal, centrifugal stopping after reaching Preset Time, and automatic calibration location.
(8) waste liquid arm 171 forwards to above film-making dish 11, and 4 waste liquid pins 172 extract the waste liquid in 4 photo making cups 14 corresponding to position simultaneously, forward the 3rd service sink 173 position immediately to and clean waste liquid pin 172 inside and outside wall.By that analogy, the waste liquid in all photo making cups 14 is extracted.
(9) arm 181 that dyes goes to above film-making dish 11, and 2 photo making cups 14 that 2 entry needles 182 are simultaneously corresponding to position add coloring agent.By that analogy, in all photo making cups 14, coloring agent is added.
(10) film-making dish 11 low speed horizontal centrifugal, makes reagent fully mix with cell and pathogen, centrifugal stopping after reaching Preset Time, and automatic calibration location.
(11) waste liquid arm 171 forwards to above film-making dish 11, and 4 waste liquid pins 172 extract the waste liquid in 4 photo making cups 14 corresponding to position simultaneously, forward the 3rd service sink 173 position immediately to and clean waste liquid pin 172 inside and outside wall.By that analogy, the waste liquid in all photo making cups 14 is extracted.
(12) (9)-(11) step is repeated according to different coloration requirements.(for Papanicolaou's vaginal smear technique): 95% alcohol fixation, 10 minutes → haematoxylin dyeing vacuole dye 2-3 minute after film-making, washing 1 minute → 1% hydrochloride alcohol 10 seconds, washing 1 minute → 95% dehydration of alcohol 2 minutes → EA50 dyeing liquor 2-3 minute → 95% alcohol washes twice.
(13) system complete sample film-making and dyeing after, system sends caution sound, now can take out prepared sample and carry out microscopic examination and diagnosis.
Embodiment of the present utility model, but design concept of the present utility model is not limited thereto, and all changes utilizing this design the utility model to be carried out to unsubstantiality, all should belong to the behavior of invading the utility model protection domain.