技术领域technical field
本实用新型涉及染色装置技术领域，特别涉及一种玻片标本染色装置。The utility model relates to the technical field of dyeing devices, in particular to a staining device for slide specimens.
背景技术Background technique
在微生物学、动物学、植物学、细胞生物学、遗传学、免疫学、组织学、解剖学、生理学、病理学、病原生物学等生命科学医学领域经，为了更清晰的观察所要观察的结构，常要对玻片标本进行染色。传统的染色方法，或采用单片染色，分步进行，或用染色缸一次可染8-10片，但复染色法进行染色时，漂洗繁琐，效率低，复染后仍要进行漂洗，染液浪费严重，且对环境造成污染。In the fields of life science and medicine such as microbiology, zoology, botany, cell biology, genetics, immunology, histology, anatomy, physiology, pathology, pathogenic biology, etc., in order to observe the structure to be observed more clearly , often stained slide specimens. The traditional dyeing method adopts single-piece dyeing, which is carried out step by step, or dyes 8-10 pieces at a time with a dyeing tank. However, when dyeing by the counter-staining method, the rinsing is cumbersome and the efficiency is low. After the counter-staining, rinsing is still required. The waste of liquid is serious and pollutes the environment.
近年来为了提高染色效率，研究人员研究制造了全自动染色制片装置，如公开号为“CN 102213655 A”的实用新型专利公开了一种全自动微生物染色制片装置，具有一柜体，柜体内腔具有洗涤池，柜体顶部之外固定有电机，电机和洗涤池之间连接有洗涤池提升机构，进出水管从柜体外部伸入洗涤池且在进出水管上分别设进出水管电磁阀；洗涤池内侧壁上设有微型液位传感器；柜体内腔顶部设有环状紫外灯且内侧面上设有一对集尘极，一对集尘极中间是空心结构的电晕极，洗涤池正上方依次是同轴对中的转盘式载玻片放置台和多孔滴染盘，每一漏斗形试剂容器底部均连通有微型电磁阀的输出管路；可对柜体内灭菌、通风和除尘。In recent years, in order to improve the dyeing efficiency, researchers have researched and manufactured a fully automatic dyeing film-making device. For example, the utility model patent with the publication number "CN 102213655 A" discloses a fully automatic microbial dyeing film-making device, which has a cabinet, a cabinet There is a washing pool in the inner cavity, and a motor is fixed outside the top of the cabinet. A washing pool lifting mechanism is connected between the motor and the washing pool. There is a miniature liquid level sensor on the inner wall of the pool; there is a ring-shaped ultraviolet lamp on the top of the cabinet cavity and a pair of dust collecting electrodes on the inner surface. The middle of the pair of dust collecting electrodes is a corona electrode with a hollow structure, directly above the washing pool. In turn, there are coaxially centered turntable glass slide placement table and porous dripping tray. The bottom of each funnel-shaped reagent container is connected with the output pipeline of miniature solenoid valve; it can sterilize, ventilate and remove dust in the cabinet.
现有的玻片标本染色装置结构复杂、成本高，不利于普遍适用。The existing slide specimen staining device has complex structure and high cost, which is not conducive to universal application.
实用新型内容Utility model content
本实用新型提供了一种玻片标本染色装置，为解决现有技术中玻片标本染色装置染色效率低、结构复杂、成本高或使用不便的问题。The utility model provides a glass slide sample dyeing device, which aims to solve the problems of low dyeing efficiency, complex structure, high cost or inconvenient use in the prior art.
本实用新型的技术方案为：The technical scheme of the utility model is:
一种玻片标本染色装置，包括：A slide sample staining device, comprising:
玻片放置架，主要由支架和若干层玻片放置层构成，所述玻片放置层与所述支架固定连接，最下层玻片放置层的下端面与所述支架底端面的距离至少为1cm；所述玻片放置层上设置有若干用于固定玻片的卡档，所述卡档底部不封闭；The slide rack is mainly composed of a bracket and several layers of slide layers, the slide layer is fixedly connected to the bracket, and the distance between the lower end surface of the bottom slide layer and the bottom end surface of the bracket is at least 1 cm ; The slide placement layer is provided with a number of clips for fixing the slides, and the bottom of the clip is not closed;
染色缸，侧壁上设置有染料管，所述染料管上设置有开关阀；Dyeing vat, the side wall is provided with a dye pipe, and the dye pipe is provided with a switching valve;
漂洗缸，第一侧面上设置有进液管，第二侧面上设置有出液管，所述进液管和出液管上均设置有开关阀。卡档底部不封闭可以使多余的染料通过底部流掉。In the rinsing cylinder, a liquid inlet pipe is arranged on the first side, and a liquid outlet pipe is arranged on the second side, and an on-off valve is arranged on the liquid inlet pipe and the liquid outlet pipe. The bottom of the stopper is not closed so that excess dye can flow out through the bottom.
优选的，所述卡档的长度等于玻片长边的长度，所述卡档的宽度等于玻片的厚度，所述卡档底部设置有玻片托，所述玻片托用于托住玻片以防止玻片掉落，所述玻片托位于所述卡档的两端。制好片后，将玻片竖直放置于直接插入卡档即可，操作方便，利于提高放片效率。Preferably, the length of the clamp is equal to the length of the long side of the slide, the width of the clamp is equal to the thickness of the slide, and a slide holder is provided at the bottom of the clamp, and the slide holder is used to hold the slide. To prevent the slides from falling, the slide holders are located at both ends of the clamp. After making the slides, just place the slides vertically and directly insert them into the card holder, which is easy to operate and helps to improve the efficiency of placing slides.
进一步的，所述玻片托的长度为所述卡档长度的1/10~1/3。卡档底部的中间位置镂空，将玻片放置架由染色缸中拿出时，多余的染液由卡档底部流掉，大大减少破片携带染液量，节约染液同时使得染色更加均匀。Further, the length of the slide holder is 1/10~1/3 of the length of the clip. The middle position of the bottom of the card holder is hollowed out. When the slide rack is taken out of the staining vat, the excess dye solution will flow off from the bottom of the card holder, which greatly reduces the amount of dye solution carried by the fragments, saves the dye solution and makes the staining more uniform.
优选的，相邻两层玻片放置层之间的距离为玻片宽度的2~5倍。留出足够空间进行操作。Preferably, the distance between the placement layers of two adjacent layers of slides is 2 to 5 times the width of the slides. Allow enough room for manipulation.
作为优选，所述玻片放置架上设置有把手。便于由染色缸或漂洗缸取出玻片放置架。Preferably, a handle is provided on the slide rack. It is convenient to remove the slide holder from the staining or rinsing tank.
优选的，所述漂洗缸底部设置有用于固定所述玻片放置架的凹槽。防止在漂洗液冲力作用下移动。Preferably, the bottom of the rinsing tank is provided with a groove for fixing the slide rack. Prevents movement under rinse fluid impulse.
使用本实用新型的玻片标本染色装置时，将制好的玻片一一放置于玻片放置架的卡档上，每个卡档放置一个玻片，玻片放置架包括多层玻片放置层，每层玻片放置层均包括若干卡档，因此整个玻片放置架上可放置大量玻片。将放置有玻片的玻片放置架整体置入染色缸中，染色缸中的染液要覆盖所有玻片，染色完毕后，将装有经染色玻片的玻片放置架再置入漂洗缸，打开进液阀和出液阀（通入的液体为漂洗液，一般为水），一边进液一边出液，直至出液无色后，取出玻片放置装置，玻片染色完成，漂洗过程中流出的漂洗液流入储液罐，便于集中处理再利用。漂洗过程中为了防止玻片放置架在液体冲力作用下移动，在漂洗缸底部设置可以容置玻片放置架支架的凹槽。When using the staining device for slide specimens of the present utility model, place the prepared slides one by one on the stalls of the slide rack, place a slide on each rack, and the slide rack includes multi-layer slides. Layers, each layer of slide placement layer includes several card files, so a large number of slides can be placed on the entire slide placement rack. Place the slide rack with the slides in the staining tank as a whole, the dye solution in the staining tank should cover all the slides, after staining, put the slide rack with the stained slides into the rinse tank , open the liquid inlet valve and the liquid outlet valve (the liquid that is passed in is the rinsing liquid, generally water), and the liquid is discharged while the liquid is in, until the liquid is colorless, take out the slide placement device, the staining of the slide is completed, and the rinsing process The rinsing liquid flowing out of the tank flows into the liquid storage tank, which is convenient for centralized treatment and reuse. In order to prevent the slide rack from moving under the force of the liquid during the rinsing process, a groove that can accommodate the slide rack bracket is provided at the bottom of the rinsing tank.
本实用新型的有益效果为：The beneficial effects of the utility model are:
本实用新型的玻片标本染色装置，结构简单、成本低、操作方便，一次性可对大量玻片染色，染色效率高。The glass slide sample staining device of the utility model has the advantages of simple structure, low cost and convenient operation, can dye a large number of slides at one time, and has high staining efficiency.
附图说明Description of drawings
为了更清楚地说明本实用新型实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本实用新型的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动性的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description These are only some embodiments of the utility model, and those skilled in the art can also obtain other drawings according to these drawings without any creative effort.
图1为本实用新型一种玻片标本染色装置中玻片放置架的结构示意图；Fig. 1 is a structural schematic diagram of a glass slide placement rack in a glass slide specimen staining device of the present invention;
图2为本实用新型一种玻片标本染色装置中漂洗缸的结构示意图；Fig. 2 is a structural schematic diagram of a rinsing tank in a slide specimen staining device of the present invention;
图3为本实用新型一种玻片标本染色装置中染色缸的结构示意图；Fig. 3 is a schematic structural view of a staining tank in a slide specimen staining device of the present invention;
图4为玻片放置架中玻片放置层的俯视结构示意图；Fig. 4 is a top view structural schematic diagram of the slide placement layer in the slide placement rack;
图5为玻片放置架中玻片放置层的仰视结构示意图；Fig. 5 is a schematic view of the upward structure of the slide placement layer in the slide placement rack;
1-玻片放置架；11-支架；12-玻片放置层；121-玻片放置层上表面；122-玻片放置层下表面；123-卡档；124-玻片托；13-把手；2-漂洗缸；21-进液阀；22-进液管；23-出液管；24-出液阀；25-凹槽；3-染色缸；31-开关阀；32-染料管。1- slide rack; 11- bracket; 12- slide layer; 121- upper surface of slide layer; 122- lower surface of slide layer; 123- clip; 124- slide holder; 13- handle 2-rinsing tank; 21-inlet valve; 22-inlet pipe; 23-outlet pipe; 24-outlet valve; 25-groove; 3-dyeing cylinder;
具体实施方式Detailed ways
实施例1Example 1
如图1-图5所示，一种玻片染色装置，包括玻片放置架1、染色缸3和漂洗缸2。As shown in FIGS. 1-5 , a slide staining device includes a slide rack 1 , a staining vat 3 and a rinsing vat 2 .
如图1、图4、图5所示，玻片放置架1包括支架11、三层玻片放置层12，支架11上还设置有把手13。三层玻片放置层12均与支架11固定连接，且三层玻片放置层12平行设置，相邻两层玻片放置层12之间的距离为玻片宽度的2~5倍。最下层玻片放置层12的底面与支架11底端面之间的距离为3~5cm。As shown in FIG. 1 , FIG. 4 , and FIG. 5 , the slide placement rack 1 includes a bracket 11 and three slide placement layers 12 , and a handle 13 is also arranged on the bracket 11 . The three layers of slide placement layers 12 are all fixedly connected to the support 11, and the three slide placement layers 12 are arranged in parallel, and the distance between two adjacent slide placement layers 12 is 2 to 5 times the width of the slide. The distance between the bottom surface of the bottom slide placement layer 12 and the bottom end surface of the support 11 is 3-5 cm.
如图4、图5所示，每层玻片放置层12上设置有若干卡档123，由玻片放置层12的上表面121看，玻片放置层12上有若干漏孔，这些漏孔分两排分布，这些漏孔用于容置玻片，即为卡档123，卡档123的长度等于玻片长边的长度，卡档123的宽度等于玻片厚度，卡档123的深度为玻片宽度的1/10~1/6。为了防止玻片掉落，玻片放置层12的下表面122设置有玻片托124，每个卡档123的两个端部分别设置玻片托124，玻片托124的长度为卡档123长度的1/10~1/3。As shown in Fig. 4 and Fig. 5, each layer of slide placement layer 12 is provided with some retaining blocks 123, seen from the upper surface 121 of the slide placement layer 12, there are some leakage holes on the slide placement layer 12, these leakage holes Distributed in two rows, these leakage holes are used to accommodate the glass slides, which are the clamps 123, the length of the clamps 123 is equal to the length of the long side of the slides, the width of the clamps 123 is equal to the thickness of the slides, and the depth of the clamps 123 is 1/10~1/6 of the slide width. In order to prevent the slides from falling, the lower surface 122 of the slide placement layer 12 is provided with slide holders 124, and the two ends of each clamp 123 are respectively provided with slide holders 124, and the length of the slide holders 124 is the length of the clamps 123. 1/10~1/3 of the length.
如图3所示，染色缸3侧面上设置有染料管32，染料管32上包括开关阀31，当染色缸3中的染料不足以淹没玻片时，向染色缸3中加入染料。As shown in Figure 3, a dye tube 32 is arranged on the side of the staining vat 3, and the dye tube 32 includes a switching valve 31. When the dye in the staining vat 3 is not enough to submerge the glass slide, dye is added to the staining vat 3.
如图2所示，漂洗缸2右侧面上的上半部分设置有进液管22，进液管22上设置进液阀21，漂洗缸2左侧面的下半部分（最好接近底部）设置有出液管23，出液管23上设置有出液阀24；漂洗缸2的底部还设置有容置支架11的凹槽25。As shown in Figure 2, the upper half on the right side of the rinsing cylinder 2 is provided with a liquid inlet pipe 22, and the liquid inlet valve 21 is arranged on the liquid inlet pipe 22, and the lower half of the left side of the rinsing cylinder 2 (preferably close to the bottom) ) is provided with a liquid outlet pipe 23, and a liquid outlet valve 24 is arranged on the liquid outlet pipe 23; the bottom of the rinsing cylinder 2 is also provided with a groove 25 for accommodating the bracket 11.
将制好的玻片一一放置于玻片放置架1的卡档123上，每个卡档123放置一个玻片，玻片放置架1包括多层玻片放置层12，每层玻片放置层12均包括若干卡档123，因此整个玻片放置架1上可放置大量玻片。将放置有玻片的玻片放置架1整体置入染色缸3中，染色缸3中的染液要覆盖所有玻片，染色完毕后，将玻片放置架1提起，待多余染料流入染色缸3后（此过程可将玻片放置架1整体架设在染色缸3上），将装有经染色玻片的玻片放置架1再置入漂洗缸2，打开进液阀21和出液阀24（通入的液体为漂洗液，一般为水），一边进液一边出液，直至出液无色后，取出玻片放置架1，玻片染色完成，漂洗过程中流出的漂洗液流入储液罐，便于集中处理再利用。漂洗过程中为了防止玻片放置架在液体冲力作用下移动，在漂洗缸底部设置可以容置玻片放置架支架的凹槽。The prepared slides are placed one by one on the clips 123 of the slide rack 1, each clip 123 places a slide, the slide rack 1 includes a multi-layer slide placement layer 12, and each layer of slides is placed Layers 12 each include a number of clips 123 , so a large number of slides can be placed on the entire slide rack 1 . Put the slide rack 1 with the slides into the staining vat 3 as a whole, the dye solution in the staining vat 3 should cover all the slides, after the staining is completed, lift the slide rack 1, and wait for the excess dye to flow into the staining vat After 3 (in this process, the slide rack 1 can be erected on the staining tank 3 as a whole), put the slide rack 1 with the stained slides into the rinse tank 2, and open the liquid inlet valve 21 and the liquid outlet valve 24 (The liquid fed in is the rinsing liquid, generally water), while the liquid is being fed in and the liquid is being discharged at the same time, until the liquid is colorless, take out the slide rack 1, the staining of the slides is completed, and the rinsing liquid flowing out during the rinsing process flows into the storage tank. The liquid tank is convenient for centralized treatment and reuse. In order to prevent the slide rack from moving under the force of the liquid during the rinsing process, a groove that can accommodate the slide rack bracket is provided at the bottom of the rinsing tank.
当采用复染色法对玻片进行染色时，准备多个染色缸和漂洗缸，将玻片放置架依次放入染色缸一、漂洗缸一、染色缸二、漂洗缸二、染色缸三、漂洗缸三……直至复染完成。When using the counterstaining method to stain the slides, prepare multiple staining tanks and rinsing tanks, and put the slide racks into the first staining tank, the first rinse tank, the second staining tank, the second rinse tank, the third staining tank, and the rinse tank. Tank three...until the counterstaining is completed.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420408272.0UCN203981475U (en) | 2014-07-23 | 2014-07-23 | A kind of slide sample dyeing apparatus |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420408272.0UCN203981475U (en) | 2014-07-23 | 2014-07-23 | A kind of slide sample dyeing apparatus |
| Publication Number | Publication Date |
|---|---|
| CN203981475Utrue CN203981475U (en) | 2014-12-03 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420408272.0UExpired - Fee RelatedCN203981475U (en) | 2014-07-23 | 2014-07-23 | A kind of slide sample dyeing apparatus |
| Country | Link |
|---|---|
| CN (1) | CN203981475U (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442076A (en)* | 2016-08-29 | 2017-02-22 | 深圳艾德拉生物技术有限公司 | Automatic slide inclined standing liquid removing method and device |
| CN115639046A (en)* | 2016-01-29 | 2023-01-24 | 希森美康株式会社 | Specimen staining device, smear specimen making device, and specimen staining method |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115639046A (en)* | 2016-01-29 | 2023-01-24 | 希森美康株式会社 | Specimen staining device, smear specimen making device, and specimen staining method |
| CN106442076A (en)* | 2016-08-29 | 2017-02-22 | 深圳艾德拉生物技术有限公司 | Automatic slide inclined standing liquid removing method and device |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | Granted publication date:20141203 Termination date:20150723 | |
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