CN203479704U - Instrument for measuring optical density of microorganism suspension liquid - Google Patents

Abstract

The utility model discloses an instrument for measuring the optical density of a microorganism suspension liquid. The instrument is characterized by comprising a shell, a main circuit board, a power source, a key display panel and an optical module, wherein the key display panel is assembled on the shell; the main circuit board is arranged in an assembling cavity of the shell; the main circuit board is connected with the power source, the key display panel and the optical module and is used for receiving a key input signal, outputting a display signal, receiving a detection signal acquired by the optical module and processing data. According to the instrument, emitting light and a receiver are respectively arranged on two sides of a test tube and are not interfered by bacterium grinding glass beads at the bottom of the test tube, so that the aim of directly measuring bacterial liquid in a bacterium grinding tube is achieved, the turbidity of the bacterial liquid in the bacterium grinding tube with the glass beads can be directly measured, and the biosafety risks of operators are lowered; in addition, the a test tube holder is arranged, so that two bacterial liquid test tubes with different diameters can be measured by using the same optical path system.

Description

A kind of instrument of measuring microbial suspension optical density

Technical field

The utility model belongs to medical test technical equipment field, is specifically related to measure the instrument of microbial suspension optical density.

Background technology

In Micro biological Tests field, Maxwell turbidimetry is usually used in Bacteria Identification test and as a kind of method that roughly judges the bacterial concentration that configures before related experiment.The methodology of Maxwell turbidimetry, according to being that setting bacterial concentration is 0.5 Maxwell opacity tube, is equivalent to 1.5 * 108/ml bacterial population.Because the method itself is exactly a kind of method of estimation, and tradition adopts the mode of range estimation, inevitable subjectivity, therefore cause the uncertainty that adopts the method to prepare bacterium liquid.

Owing to preparing the accuracy of bacterial concentration, be the key factor that affects related experiment net result order of accuarcy, old friends start to research and develop Maxwell nephelometer, to obtaining bacterial concentration testing result accurately.Maxwell nephelometer is the principle based on measuring optical density, and the turbidity of bacterium is measured, and result be take Maxwell (McFarland) and with digital form, shown as unit, thereby the bacterial concentration that experimenter prepares is consistent.

Existing Maxwell nephelometer all adopts scattering method principle, and incident light and receiver are 90 ° of angles, can not meet part requirement of experiment, as the detection of Much's bacillus bacterial concentration.Because using mill tube (pipe contains little beaded glass) to grind bacterium (relying on colliding with each other, milling cenobium is broken up between in-built little beaded glass) when preparing mycobacterium bacterium liquid.Existing Maxwell structure that nephelometer adopts and principle are vulnerable to grind the impact of tube bottom glass pearl when detecting bacterial concentration, therefore cannot directly use bottom to have the mill tube of beaded glass to carry out coherent detection, and beaded glass in mill tube need to be taken out or bacteria suspension sucking-off is proceeded to after another test tube to row again, detect, not only increase operation steps but also have potential bio-safety risk.

Utility model content

The purpose of this utility model is to provide a kind of instrument of measuring microbial suspension optical density, has the turbidimetric function in existing Maxwell, also has the function that can directly carry out Maxwell turbidity detection to the bacterium liquid in mill tube.The purpose of this utility model is realized by following technical scheme:

An instrument of measuring microbial suspension optical density, is characterized in that, comprising: shell, main circuit board, power supply, button display panel and optical module; Button display panel is assemblied on shell, and main circuit board is arranged in the assembly cavity of shell; Main circuit board is connected with power supply, button display panel and optical module, receives key-press input signal, output display signal, and receive the detection signal of optical module collection and carry out data processing.

Described optical module comprises colorimetric shelf, test tube sleeve, LED spotlight, optical filter and optical receiver; Colorimetric shelf is arranged on main circuit board, and test tube sleeve is arranged on colorimetric shelf, and the shell of top, test tube sleeve present position offers test tube and places mouth; LED spotlight and optical filter are arranged on the light-path of test tube sleeve one side, and optical receiver is arranged on the light-path of the relative LED spotlight of test tube sleeve opposite side, and the output of optical receiver connects main circuit board.

As further technical scheme, described measuring instrument also comprises that test tube joins seat, and this test tube is joined seat and can be matched and be arranged on test tube sleeve; Described test tube is joined to be provided the test tube of comparing test tube sleeve and places a mouthful less test tube putting hole on seat.

As concrete technical scheme, described shell comprises lower house and upper shell, and both fasten, and form described assembly cavity.

As concrete technical scheme, on described lower house, be provided with battery compartment, described power supply comprises battery and power switch, and battery is arranged in described battery compartment, and power switch is controlled the keying of battery supply output.

As further technical scheme, described measuring instrument also comprises top cover, and lid is located at described test tube and places a mouthful top.

The utility model is improved on transmission beam method principle basis, utilizing emitted light and receiver split test tube both sides, be not subject to the interference of bottom mill bacterium beaded glass, to reach the object that the bacterium liquid in mill tube is directly measured, can directly to the mill tube of beaded glass is housed, carry out bacterium liquid turbidimetry, reduce operator's bio-safety risk; In addition, by test tube is set, join seat, realize same light path system the bacterium liquid test tube of two kinds of different tube diameters is measured.

Accompanying drawing explanation

The structural map of the instrument of the measurement microbial suspension optical density that Fig. 1 provides for embodiment.

The structural map of optics module in the instrument of the measurement microbial suspension optical density that Fig. 2 provides for embodiment.

Embodiment

As shown in Figure 1, the instrument of the measurement microbial suspension optical density that the present embodiment provides, comprising:shell 10,top cover 20,main circuit board 30,power supply 40,button display panel 50 andoptical module 60.

Shell 10 comprises lower house 11 andupper shell 12, and both fasten, and form an assembly cavity.Onupper shell 12 front end faces, assemblebutton display panel 50, top offers test tube and places mouth, and the top that this test tube picks and places mouth arranges top cover 20.On lower house 11, be provided with battery compartment,power supply 40 comprisesbattery 41 andpower switch 42, andbattery 41 is arranged in battery compartment, and power switch is controlled the keying ofbattery 41 power supply outputs.

Main circuit board 30 is arranged in the assembly cavity ofshell 10, is integrated with microcomputer control system on main circuit board.Main circuit board 30 is connected withpower supply 40,button display panel 50 andoptical module 60, correspondingly receives key-press input signal, output display signal, and receive the detection signal ofoptical module 60 collections and carry out data processing.

Optical module 60 is arranged on main circuit board, and as shown in Figure 2, it comprises thatcolorimetric shelf 61,test tube sleeve 62, testtube join seat 63,LED spotlight 66, optical filter and optical receiver 64.Colorimetric shelf 61 is arranged onmain circuit board 30, andtest tube sleeve 62 is arranged oncolorimetric shelf 61, provides a larger test tube putting hole on test tube sleeve 62.Test tube is joinedseat 63 and can be matched and be arranged ontest tube sleeve 62, and test tube is joined onseat 63 provides a less test tube putting hole.On the upper shell oftest tube sleeve 62 tops, present position, offer described test tube and placemouth.LED spotlight 66 and optical filter are arranged on the light-path of side oftest tube sleeve 62, andoptical receiver 64 is arranged on the light-path of side oftest tube sleeve 62 of the relative side ofLED spotlight 66, and the output ofoptical receiver 64 connectsmain circuit board 30.

During detection, larger test tube is directly positioned intest tube sleeve 62, and less test tube is joinedseat 63 by test tube and is positioned intest tube sleeve 62, and test tube bottom is positioned in the light path ofLED spotlight 66; The light thatLED spotlight 66 sends, by optical filter, injects from test tube one side, from opposite side, penetrates by optical receiver acknowledge(ment) signal; Microcomputer control system integrated onmain circuit board 30 can carry out the choice of diameter to instrument, adopts corresponding algorithm to carry out turbidity calculating to the photosignal collecting, and turbidity value is exported according to caliber parameter.

The instrument of the measurement microbial suspension optical density that the present embodiment provides, the operating process of an one concrete application is as follows:

(1) operating personnel make a certain amount of bacterium liquid with mill tube or other test tubes;

(2) by the switch key of operation push-button display panel 50, open instrument, display shows the menu interface that main circuit board provides, and selects the corresponding caliber of pre-detection test tube;

(3) openupper cover 20, mill tube or other cuvette cartridge bacterium liquid dilutions are inserted in thetest tube sleeve 62 ofoptical module 60;

(4) by the value of the reading button of operation push-button display panel 50, theLED spotlight 66 ofoptical module 60 sends light source, and light, by the optical filter of certain wavelength, is injected from test tube one side, through bacterium liquid, from test tube opposite side, penetrate and carry out signal reception byoptical receiver 64;

(5)optical receiver 64 is converted into electric signal by the light signal of reception, offersmain circuit board 30, and microcomputer control system integrated onmain circuit board 30 calculates, and result is exported with Maxwell turbidity unit, is presented at display, completes detection;

(6), after having detected, by operating described on & off switch, exit instrument system, powered-down.

Above embodiment is only for fully open unrestricted the utility model, for the simple replacement that can know without creative work, or the increase and decrease of constructed feature, should belong to the application scope of disclosure.

Claims (5)

1. an instrument of measuring microbial suspension optical density, is characterized in that, comprising: shell, main circuit board, power supply, button display panel and optical module; Button display panel is assemblied on shell, and main circuit board is arranged in the assembly cavity of shell; Main circuit board is connected with power supply, button display panel and optical module, receives key-press input signal, output display signal, and receive the detection signal of optical module collection and carry out data processing;

Described optical module comprises colorimetric shelf, test tube sleeve, LED spotlight, optical filter and optical receiver; Colorimetric shelf is arranged on main circuit board, and test tube sleeve is arranged on colorimetric shelf, and the shell of top, test tube sleeve present position offers test tube and places mouth; LED spotlight and optical filter are arranged on the light-path of test tube sleeve one side, and optical receiver is arranged on the light-path of the relative LED spotlight of test tube sleeve opposite side, and the output of optical receiver connects main circuit board.

2. the instrument of measurement microbial suspension optical density according to claim 1, is characterized in that, described measuring instrument also comprises that test tube joins seat, is arranged on test tube sleeve; Described test tube is joined to be provided the test tube of comparing test tube sleeve and places a mouthful less test tube putting hole on seat.

3. the instrument of measurement microbial suspension optical density according to claim 1, is characterized in that, described shell comprises lower house and upper shell, and both fasten, and form described assembly cavity.

4. the instrument of measurement microbial suspension optical density according to claim 3, it is characterized in that, on described lower house, be provided with battery compartment, described power supply comprises battery and power switch, battery is arranged in described battery compartment, and power switch is controlled the keying of battery supply output.

5. the instrument of measurement microbial suspension optical density according to claim 1, is characterized in that, described measuring instrument also comprises top cover, and lid is located at described test tube and places a mouthful top.

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