CN203037655U - Rapid detection card for carbendazim - Google Patents

Abstract

The utility model discloses a rapid detection card for carbendazim, and belongs to the technical field of carbendazim detection. A testing strip is arranged in a housing of the rapid detection card for the carbendazim, and is formed by sequentially pasting a sample cushion, a colloidal gold (or an emulsion particle) membrane, a nitrocellulose membrane and a water absorption membrane on a bearing back plate; the colloidal gold (or the emulsion particle) membrane is a glass fiber membrane containing a carbendazim-resistant antibody colloidal gold (or emulsion particle) marker; the nitrocellulose membrane is provided with two detection straps transversely; one is a detection strap containing a carbendazim protein conjugate; and the other one is a quality control strap containing a rabbit-resistant antibody or a mouse-resistant antibody. The rapid detection card has the advantages that the rapid detection card applies an immunological method to detect the residual quantity of the carbendazim in samples of vegetable and fruit such as rice, wheat, cucumbers, apples and pears directly. The rapid detection card for the carbendazim is easy to fabricate, convenient and rapid to use, and accurate in result.

Description

A kind of carbendazim rapid detection card

Technical field

The utility model relates to a kind of carbendazim rapid detection card, is a kind of detection utensil that detects carbendazim, belongs to carbendazim detection technique field.

Background technology

Carbendazim has another name called No. 44, carbendazol, benzo miaow.Carbendazim is a kind of broad-spectrum germicide, and various crop is had prevention effect by the disease that fungi (as Fungi Imperfecti, many sac fungus) causes.

At present, the carbendazim residue detection mainly is to use high performance liquid chromatography and LC-MS etc. to carry out qualitative and quantitative analysis.These methods require high to experimental instrument and equipment, complex operation is high to operating personnel's professional knowledge requirement, wastes time and energy.Therefore set up and a kind ofly detect quickly and easily again accurately and reliably that the analytical approach of carbendazim is very important in the food.

The utility model is sent out bright a kind of based on having antigenic immunological detection method after the small-molecule substance carbendazim coupling protein matter, take immunology competition ratio juris, a kind of quick detection reagent of association colloid gold (or latex particle) labelling technique and immunochromatographic method design can detect the carbendazim in the vegetables and fruits samples such as rice, wheat, cucumber, apple, pears fast specifically.Carbendazim content in the sample is judged in the colour developing that has or not according to test card sample detection line (T) and nature controlling line (C).Reaction is to utilize carbendazim protein conjugate antigenicity and a kind of detection method of setting up, has the advantage of high specificity, the flase drop problem that can avoid the false positive of other detection method to cause.Test card is simple to operate simultaneously need not special instrument and equipment fast, is fit to execute-in-place.Because sample size needs less, easy fast, be applicable to the examination of great amount of samples.The result is accurate, and is highly sensitive, and accuracy rate is big.

Summary of the invention

The purpose of this utility model is to provide a kind of detection utensil that can detect carbendazim, directly the method for applied immunology detects the content of carbendazim in the vegetables and fruits samples such as rice, wheat, cucumber, apple, pears, overcome the shortcomings such as complex operation that existing chromatography detects, set up based on the specific detection method of immunology.

The technical solution of the utility model: a kind of carbendazim rapid detection card, it is for arranging test-strips 2 in the test card shell 1 of rectangular flat shell shape, and the test card case surface has thefenestra 3 of detection and well 4; Test-strips 2 is formed by pasting sample pad 8, collaurum (or latex particle) film 7, nitrocellulose filter 6 and absorbingmembrane 9 thereon successively by adhesive sticker on the supporting backboard 5; The stacked stickup nitrocellulose filter 6 in supporting backboard middle part, one endlap of supporting backboard is put and is pasted absorbingmembrane 9, the stacked stickup sample pad 8 of the other end, absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum (or latex particle) film 7; Collaurum (or latex particle) film is for containing the glass fibre membrane of resisting carbendazim antibody colloidal gold (or latex particle) mark, there is one to detect band and a quality control band on the nitrocellulose filter, be successively: the detection that contains the carbendazim protein conjugate is with 10, thequality control band 11 that contains anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell, sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.

The beneficial effects of the utility model: the utility model has the advantages that the carbendazim that contains in the vegetables and fruits samples such as can detecting rice, wheat, cucumber, apple, pears, be easy to preparation and save testing cost, easy to use, detect fast, highly sensitive, the result is accurate.

Description of drawings

Fig. 1 carbendazim rapid detection card shell and test-strips arrangement figure in the enclosure.1, shell, 2, test-strips, 3, detect fenestra, 4, well, 5, the supporting backboard.

Fig. 2 detects and shows the trace synoptic diagram on the nitrate glass tunica fibrosa of strip.6, nitrocellulose filter, 10, detect band, 11, quality control band.

The detection strip structural representation of Fig. 3 carbendazim rapid detection card.5, supporting backboard, 6, nitrocellulose filter, 7, collaurum (or latex particle) film, 8, sample pad, 9, absorbing membrane.

Embodiment

The utility model further specifies as follows in conjunction with specific embodiments referring to accompanying drawing:

Embodiment 1

A kind of carbendazim rapid detection card, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1, test card shell 1 is rectangular flat shell shape shell, long 70mm, wide 20mm, thick 5mm, shell wall thickness 1mm is made by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and at test card shell 1 cap thefenestra 3 of detection and well 4 is arranged.Test-strips 2 is placed in the shell block of test card shell 1.

Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 60mm, the wide about 4mm of sheet, the about 2.5mm of thickness.Test-strips 2 is sandwich constructions: bottom is supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 60mm, wide 4mm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 20-25mm, wide 4mm.Two isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, show in the trace band referring to 2, two in accompanying drawing, one is to detect to be with 10, contains the carbendazim protein conjugate; Another isquality control band 11, contains anti-rabbit antibody or anti-mouse antibody.Stackedstickup absorbing membrane 9 on supporting backboard 5 one ends, stacked stickup sample pad 8 on supporting backboard 5 the other end, absorbingmembrane 9 the inners overlap with nitrocellulose filter 6 one ends respectively separately with sample pad 8 the inners, at the clinch of sample pad 8 with nitrocellulose filter 6, both asking inserts and puts stickup one section collaurum (or latex particle) film 7, and collaurum (or latex particle) film 7 is the glass fibre membranes that contain resisting carbendazim antibody colloidal gold (or latex particle) label.Collaurum (or latex particle) film 7 thick 0.5-0.8mm, long 5mm, wide 4mm.Sample pad 8 thick 0.5-0.8mm, long 20mm, wide 4mm.Absorbing membrane 9 thick 0.5-0.8mm, long 16-20mm, wide 4mm.Collaurum (or latex particle) film 7, sample pad 8 and absorbingmembrane 9 are 1-2mm with the nitrocellulose filter lap of splice.Absorbing membrane 9 materials are thieving paper, and sample pad 8 materials are water adsorption glass tunica fibrosas.

The utility model carbendazim test card is for detection of the detection of the many mattresses spirit in the vegetables and fruits samples such as rice, wheat, cucumber, apple, pears.

Embodiment 2

The disposal route of sample is as follows:

Take by weighing the 50.0g chopping, the sample of mixing, add 50ml methyl alcohol jolting 0.5h, use the Buchner funnel suction filtration, container and filter methanol wash twice, each 15-20ml, after draining, filtrate moves in the beaker, the about 10mL water washing of bottle,suction, and washing lotion is incorporated in the filtrate, after blowing away part methyl alcohol with air stream in the water-bath, move in the separating funnel, add 30mL sodium chloride solution (100g/L), extract secondary with the sherwood oil jolting, each 25mL, discard sherwood oil, add hcl acidifying to pH be 1-2 (using the pH test paper), extract twice with methylene chloride, each 25mL, the combined dichloromethane extract with the 25mL water washing once, is told dichloromethane layer, water washing liquor is integrated with water layer, gives over to carbendazim and measures usefulness.

When using the carbendazim rapid detection card to detect, earlier sample liquid to be detected is instilled into from well 4 on the sample pad 8 of carbendazim rapid detection card, because syphonic effect principle, drive contained resisting carbendazim antibody colloidal gold (or latex particle) labels of sample liquid to be detected and collaurum (or latex particle) film 7 together to nitrocellulose filter 6 diffusions, observations in 5-10 minute.

The key reaction of test card is immunologic antigen and antibody response, the antibody of collaurum (or latex particle) mark that moves at nitrocellulose filter, on calibration tape respectively with contain the carbendazim protein conjugate, and the anti-rabbit antibody of quality control band or anti-mouse antibody reaction forms the brownish red band.If have carbendazim to be checked to exist in the sample, after sample adds, namely react with antibody, and can not react with contained carbendazim protein conjugate with detection, thereby do not develop the color.The result of key reaction has following 3 kinds of situations:

1, when detection be with 10 andquality control band 11 manifest brownish red trace band simultaneously, the expression testing result is negative, illustrate to be detected that carbendazim content does not exceed standard in the sample.

2, be with 10 Show Colors not when detection, have onlyquality control band 11 to manifest the brownish red trace, testing result is positive, and carbendazim content overproof in the detected sample is described.

3, when detection be with 10 and quality control band 12 all do not develop the color, show that then detecting strip lost efficacy.

Claims (1)

1. carbendazim rapid detection card, it is characterized in that: in the test card shell (1) of rectangular flat shell shape test-strips (2) is set, the test card case surface has detection fenestra (3) and well (4); Test-strips (2) is upward pasted sample pad (8), collaurum or latex particle film (7), nitrocellulose filter (6) and absorbing membrane (9) thereon successively by adhesive sticker by supporting backboard (5) and is formed; Supporting backboard middle part stacked stickup nitrocellulose filter (6), one endlap of supporting backboard is put and is pasted absorbing membrane (9), the stacked stickup sample pad of the other end (8), absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum or latex particle film (7); Collaurum or latex particle film are the glass fibre membrane that contains resisting carbendazim antibody colloidal gold or latex particle mark, there is one to detect band and a quality control band on the nitrocellulose filter, be successively: the detection band (10) that contains the carbendazim protein conjugate, the quality control band (11) that contains anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell, sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.

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