CN202576435U

Movatterモバイル変換

Info

Publication number: CN202576435U
Application number: CN 201220205538
Authority: CN
Inventors: 蒋东阳; 伍活镰; 尹顺义; 裴舜
Original assignee: GUANGZHOU QIZHI BIOLOGY ENGINEERING EQUIPMENT Co Ltd
Current assignee: GUANGZHOU QIZHI BIOLOGY ENGINEERING EQUIPMENT Co Ltd
Priority date: 2012-05-08
Filing date: 2012-05-08
Publication date: 2012-12-05
Anticipated expiration: 2022-05-08

Abstract

The utility model discloses an aeration device for a bioreactor, comprising two sprinkler head connectors and a plurality of hollow cylindrical screen clothes, wherein the plurality of hollow cylindrical screen clothes are distributed between the two sprinkler head connectors in parallel, and are in sealing connection with the two sprinkler head connectors. According to the aeration device for the bioreactor, the low-shear force and high-efficiency dissolved oxygen conduction can be realized.

Description

The breather of bio-reactor

Technical field

The utility model relates to the bio-reactor field, relates more specifically to a kind of breather of bio-reactor.

Background technology

During cultivating, mass cell exists dissolved oxygen conduction and the not good problem of shearing force always.Wherein, the raising of dissolved oxygen conduction can reach through improving rotating speed, or reaches through liquid gas exchange area; Shearing force then is through the reduction rotating speed, or realizes flowing of liquid through pump, pressure.Conflict because of mutual through the enforcement schemes of rotating speed between the two, be difficult to perfectly solved.

And if the expansion that dissolved oxygen conducts through liquid gas exchange area improves, though the mode that adopts at present is a lot, agent technology still reduces two kinds:

1, reduces the volume of bubble, generally be referred to as the microvesicle ventilation.It is huge equally in the break shearing force brought of liquid level that shortcoming is a bubble, and this liquid gas interface tensile effect meeting attaches a large amount of cells, increased the weight of the damage of pair cell.Cultivate if be applied to microcarrier, also can go out the dry possibility of expression vector come-up.

2,, can realize increasing the utmost point low-shearing power that liquid gas exchange area again can still ventilation through the huge surface area of films such as silica gel, tubular fibre.But shortcoming also clearly, this film is usually very fragile, does not also improve what even add supporter, moreover film body adds to make the matter worse especially afterwards originally with regard to easy blocking.

In the large scale culturing, also there is not to realize the function of online aseptic replacing detecting electrode up to now.Usually because fragile detecting electrode receives influence and the strike ahead of time of itself and operating mode in life-span, thereby having to stop causes immeasurable loss when batch production.

The utility model content

The purpose of the utility model provides a kind of breather of having realized the bio-reactor of low-shearing power and highly effective oxygen dissolving conduction.

For realizing above-mentioned purpose; The utility model provides a kind of breather of bio-reactor; Comprise two sprinkler head junctors and a plurality of hollow cylindrical screen cloth; Said a plurality of hollow cylindrical screen cloth is parallel and is distributed between said two sprinkler head junctors, and is tightly connected with said two sprinkler head junctors.

Preferably, said two sprinkler head junctors are horn-like, and the big opening end of said two sprinkler head junctors is equipped with a plurality of open holes, and the two ends correspondence of said hollow cylindrical screen cloth is sealingly connected to said open hole.

Preferably, the two ends of said hollow cylindrical screen cloth all are connected with pipe connecting, and said pipe connecting is seal-installed in the said open hole.

Preferably, the internal diameter of said hollow cylindrical screen cloth is greater than said pipe connecting external diameter, and said pipe connecting external diameter equals said open hole internal diameter.

Preferably, also comprise the cylinder outer cover, said cylinder outer cover is coated on the periphery of said hollow cylindrical screen cloth and said pipe connecting, and the big opening end of the two ends of said cylinder outer cover and said two sprinkler head junctors is tightly connected.

Preferably, the big opening end of said two sprinkler head junctors is equipped with the seal groove position, and the two ends correspondence of said cylinder outer cover is absorbed in the said seal groove position.

Preferably, said hollow cylindrical screen cloth and said cylinder outer cover are processed by stainless steel.

Preferably, also be equiped with glass vision panel, electrode insertion mouth and breather line on the said cylinder outer cover.

Preferably, the osculum end of said two sprinkler head junctors is connected to quick aseptic X-over or diaphragm valve group.

Compared with prior art; The breather of the bio-reactor of the utility model is owing to be provided with said hollow cylindrical screen cloth; The dissolved oxygen that feasible ventilation brings etc. is delivered to mobile through said hollow cylindrical screen cloth and contains in the developing medium of culture, the shearing force harm of having avoided the ventilation bubble to bring.

Through following description and combine accompanying drawing, it is more clear that the utility model will become, and these accompanying drawings are used to explain the embodiment of the utility model.

Description of drawings

Fig. 1 is the synoptic diagram of the breather of the utility model bio-reactor.

Embodiment

With reference now to accompanying drawing, describe the embodiment of the utility model, the similar elements label is represented similar elements in the accompanying drawing.

Please refer to Fig. 1, the breather of described bio-reactor comprises two sprinkler head junctors 1, cylinder outer cover 2, a plurality of pipe connecting and an a plurality of hollow cylindrical screen cloth 4.

Said two sprinkler head junctors 1 are horn-like; Have big opening end and osculum end; The big opening end of said two sprinkler head junctors 1 is equipped with a plurality of open holes (preferably; The quantity of said open hole is 4-10) and the seal groove position, the seal groove position is a groove position that is approximately higher than O RunddichtringO line footpath, the osculum end of said two sprinkler head junctors 1 is connected to quick aseptic X-over or diaphragm valve group.

Said cylinder outer cover 2 stainless steels are processed; It is located between said two sprinkler head junctors 1; And be coated on the periphery of said hollow cylindrical screen cloth 4 and said pipe connecting, the two ends correspondence of said cylinder outer cover 1 is absorbed in the said seal groove position and the big opening end of said two sprinkler head junctors 1 is tightly connected.Said cylinder outer cover 2 upsides are equiped with glass vision panel 5 and observe and electrode insertion mouth 3 with convenient, and said cylinder outer cover 2 downsides are equiped with breather line 6.

Preferably; The quantity of said pipe connecting is 4-10; Said pipe connecting is connected in the two ends of said hollow cylindrical screen cloth 4, and is connected in the said open hole, and said pipe connecting external diameter equals said open hole internal diameter; Said pipe connecting external diameter is less than the internal diameter (external diameter of said hollow cylindrical screen cloth 4 is greater than more than the said pipe connecting external diameter 5mm) of said hollow cylindrical screen cloth 4, and the outside of said pipe connecting is with the terpolymer EP rubber O type circle as sealing element.

Preferably, the quantity of said hollow cylindrical screen cloth 4 is 4-10, and this 4-10 hollow cylindrical screen cloth 4 is parallel and is distributed between said two sprinkler head junctors 1, and is tightly connected through said pipe connecting and said two sprinkler head junctors 1.Said hollow cylindrical screen cloth 4 is processed by stainless steel.

The breather of said bio-reactor can be positioned at the flow through optional position of pipeline of developing medium; So can be through using quick aseptic X-over or on big volume reactor, being equipped with the diaphragm valve group also easily realizes the breather of the said bio-reactor of on-line replacing with sterilization method on the throne possibility, the on-line replacing detecting electrode has also just been realized equally.

As stated, because said hollow cylindrical screen cloth 4 is set, the dissolved oxygen that making ventilates brings etc. is delivered to mobile through said hollow cylindrical screen cloth 4 and contains in the developing medium of culture the shearing force harm of having avoided the ventilation bubble to bring.

More than combine most preferred embodiment that the utility model is described, but the utility model is not limited to the embodiment of above announcement, and should contains various modification, equivalent combinations of carrying out according to the essence of the utility model.

Claims

1. the breather of a bio-reactor; It is characterized in that; Comprise: two sprinkler head junctors and a plurality of hollow cylindrical screen cloth, said a plurality of hollow cylindrical screen clothes are parallel and are distributed between said two sprinkler head junctors, and are tightly connected with said two sprinkler head junctors.

2. the breather of bio-reactor as claimed in claim 1; It is characterized in that: said two sprinkler head junctors are horn-like; The big opening end of said two sprinkler head junctors is equipped with a plurality of open holes, and the two ends correspondence of said hollow cylindrical screen cloth is sealingly connected to said open hole.

3. the breather of bio-reactor as claimed in claim 2, it is characterized in that: the two ends of said hollow cylindrical screen cloth all are connected with pipe connecting, and said pipe connecting is seal-installed in the said open hole.

4. the breather of bio-reactor as claimed in claim 3, it is characterized in that: the internal diameter of said hollow cylindrical screen cloth is greater than said pipe connecting external diameter, and said pipe connecting external diameter equals said open hole internal diameter.

5. the breather of bio-reactor as claimed in claim 3; It is characterized in that: also comprise the cylinder outer cover; Said cylinder outer cover is coated on the periphery of said hollow cylindrical screen cloth and said pipe connecting, and the big opening end of the two ends of said cylinder outer cover and said two sprinkler head junctors is tightly connected.

6. the breather of bio-reactor as claimed in claim 5, it is characterized in that: the big opening end of said two sprinkler head junctors is equipped with the seal groove position, and the two ends correspondence of said cylinder outer cover is absorbed in the said seal groove position.

7. the breather of bio-reactor as claimed in claim 5, it is characterized in that: said hollow cylindrical screen cloth and said cylinder outer cover are processed by stainless steel.

8. the breather of bio-reactor as claimed in claim 5 is characterized in that: also be equiped with glass vision panel, electrode insertion mouth and breather line on the said cylinder outer cover.

9. the breather of bio-reactor as claimed in claim 2, it is characterized in that: the osculum end of said two sprinkler head junctors is connected to quick aseptic X-over or diaphragm valve group.