CN202509077U - Microbial immobilization fermentation tank for producing 1,3-propylene glycol - Google Patents

Abstract

Translated fromChinese

本实用新型公开一种生产1,3-丙二醇的微生物固定化发酵罐，包括对应扣合的罐体和罐盖，该罐内设有用于装入固定化载体的反应管组，该罐体上设有与该反应管组的进口端和出口端对应连通的进料管和出料管；所述罐盖通过锁扣装置以能开启和盖合的方式活动设在所述罐体上，所述反应管组通过隔板以能取出和装入的方式活动装设在所述罐体内。本实用新型的生产1，3-丙二醇的微生物固定化发酵罐，通过锁扣装置能够实现罐盖与罐体的锁合功能，开启锁扣装置后打开罐盖，将活动装设在隔板上的反应管组取出，并进行更换或清洗作业，操作便利；而且其结构紧凑，节约投资成本，尤其适用于好氧或厌氧发酵生产1，3-丙二醇。

The utility model discloses a microorganism immobilized fermentation tank for producing 1,3-propanediol, which comprises a tank body and a tank cover which are fastened correspondingly. The tank is provided with a reaction tube group for loading an immobilized carrier. There are feed pipes and discharge pipes correspondingly connected with the inlet end and outlet end of the reaction tube group; the tank cover is movable on the tank body in a manner that can be opened and closed through a locking device, so The reaction tube group is movably installed in the tank body in a manner that can be taken out and loaded through a partition. The microbial immobilized fermenter for producing 1,3-propanediol of the present utility model can realize the locking function of the tank cover and the tank body through the locking device. After the locking device is opened, the tank cover is opened, and the activity is installed on the partition The reaction tube group is taken out and replaced or cleaned, and the operation is convenient; moreover, it has a compact structure and saves investment costs, and is especially suitable for producing 1,3-propanediol by aerobic or anaerobic fermentation.

Description

Translated fromChinese

生产1,3-丙二醇的微生物固定化发酵罐Microorganism Immobilized Fermenter for Producing 1,3-Propanediol

技术领域technical field

本实用新型涉及一种固定化细胞发酵罐，特别是涉及一种用于微生物发酵法生产1，3-丙二醇(1，3-propanediol，以下简称1，3-PD)的设备。 The utility model relates to an immobilized cell fermentation tank, in particular to a device for producing 1,3-propanediol (1,3-propanediol, hereinafter referred to as 1,3-PD) by a microbial fermentation method. the

背景技术Background technique

1，3-PD是一种重要的有机化工原料，可参与多种化学合成反应，可用于合成抗冻剂、防腐剂、乳化剂和多种增塑剂；最主要的用途是作为单体合成新型聚酯——聚对苯二甲酸丙二醇酯(polytrimethyleneterephthalate，以下简称PTT)。 1,3-PD is an important organic chemical raw material, which can participate in a variety of chemical synthesis reactions, and can be used to synthesize antifreeze, preservatives, emulsifiers and various plasticizers; the most important use is as a monomer synthesis A new type of polyester - polytrimethylene terephthalate (polytrimethyleneterephthalate, hereinafter referred to as PTT). the

目前，国际上1，3-PD的生产主要采用化学合成法，且都被国外的公司垄断。随着绿色工艺理念的普及，利用可再生资源为原料，通过生物法生产1，3-PD已受到极大重视，其中利用微生物发酵生产1，3-PD的工艺如美国DuPont公司与Genencor公司联合开发基因工程菌，以葡萄糖为原料的一步发酵法；德国国家生物技术中心(GBF)积极开展的以甘油为原料，通过细菌厌氧发酵法；国内有清华大学、大连理工大学、华东理工大学和华侨大学等高校相继开展生物发酵法工艺。此外生物法工艺还包括如申请号200610038136.7的发明专利申请文件中公开的“通过多酶耦合催化、辅酶再生转化甘油、3-羟基丙醛生产二羟基丙酮和1，3-PD”的方法。 At present, the production of 1,3-PD in the world mainly adopts chemical synthesis, and all of them are monopolized by foreign companies. With the popularization of the concept of green technology, the use of renewable resources as raw materials to produce 1,3-PD through biological methods has received great attention. Among them, the process of using microbial fermentation to produce 1,3-PD, such as the United States DuPont company and Genencor company. Develop genetically engineered bacteria, one-step fermentation method with glucose as raw material; German National Biotechnology Center (GBF) actively develops glycerin as raw material, through bacterial anaerobic fermentation method; domestic Tsinghua University, Dalian University of Technology, East China University of Science and Technology and Huaqiao University and other colleges and universities have successively developed biological fermentation technology. In addition, the biological process also includes the method of "production of dihydroxyacetone and 1,3-PD by multi-enzyme coupling catalysis, coenzyme regeneration conversion of glycerol and 3-hydroxypropionaldehyde" as disclosed in the invention patent application document with application number 200610038136.7. the

微生物发酵工艺较常利用固定化技术提高产品浓度和生产效率。所谓固定化技术，是指利用物理或化学手段将游离的细胞或酶与固态的不溶性载体相结合，使其保持活性并可反复使用的一种技术，主要包括固定化酶技术和固定化细胞技术。固定化生产1，3-PD的技术陆 续报道，较早是德国Pflugmacher等人固定化弗氏柠檬酸杆菌(Citrobacter freundii)；内布拉斯加州大学的Amery等人固定化丁酸梭状芽孢杆菌(Clostridium butyricum)；国内，浙江大学和华侨大学使用NaCS/PDMDAAC中空微胶囊包埋兼性厌氧菌肺炎克雷伯氏杆菌(Klebsiella pneumoniae)；江南大学用海藻酸钙固定化重组大肠杆菌发酵1，3-PD；又如申请号201010285458.8的专利申请文件中公开的“1，3-丙二醇的生物膜固定化发酵法”，该方法中发酵罐外连通至少一个填充纤维载体的生物膜反应器(体积是发酵罐体积的5-30％)，利用肺炎克雷伯氏杆菌形成生物膜进行连续发酵生产。 Microbial fermentation processes often use immobilization technology to improve product concentration and production efficiency. The so-called immobilization technology refers to a technology that uses physical or chemical means to combine free cells or enzymes with solid insoluble carriers to keep them active and reusable, mainly including immobilized enzyme technology and immobilized cell technology . The technology of immobilized production of 1,3-PD has been reported successively. Earlier, Pflugmacher et al. immobilized Citrobacter freundii in Germany; Amery et al. at the University of Nebraska immobilized Clostridium butyricum Clostridium butyricum; in China, Zhejiang University and Huaqiao University used NaCS/PDMDAAC hollow microcapsules to embed the facultative anaerobic bacteria Klebsiella pneumoniae; Jiangnan University used calcium alginate to immobilize recombinant Escherichia coli forfermentation 1,3-PD; another example is the "biofilm immobilized fermentation method of 1,3-propanediol" disclosed in the patent application document of application number 201010285458.8. In this method, at least one biofilm reactor filled with a fiber carrier is connected outside the fermenter (The volume is 5-30% of the volume of the fermenter), and the continuous fermentation production is carried out by using Klebsiella pneumoniae to form a biofilm. the

固定化达到的效果中微生物浓度和产物浓度有所提高、细胞耐环境冲击、产物较易分离、操作稳定性好，但在固定化细胞循环使用上仍存在较多问题。同时，现有的传统搅拌式通用发酵罐不适合用于固定化技术进行连续发酵生产，且由于搅拌装置的存在，制造和加工都较复杂，操作能耗也较大。因此，有必要开发一种能够适用于固定化技术进行连续生产1，3-PD的固定化发酵罐。 Among the effects achieved by immobilization, the concentration of microorganisms and products has been improved, the cells are resistant to environmental impact, the products are easier to separate, and the operation stability is good, but there are still many problems in the recycling of immobilized cells. At the same time, the existing traditional stirring general-purpose fermenter is not suitable for continuous fermentation production with immobilization technology, and due to the existence of the stirring device, the manufacturing and processing are more complicated, and the operation energy consumption is also relatively large. Therefore, it is necessary to develop an immobilized fermenter suitable for continuous production of 1,3-PD by immobilization technology. the

实用新型内容Utility model content

有鉴于此，本实用新型的目的在于提供一种生产1，3-丙二醇的微生物固定化发酵罐，能用于连续发酵生产1，3-丙二醇且实用高效，尤其可用于厌氧过程。 In view of this, the purpose of this utility model is to provide a microbial immobilized fermenter for producing 1,3-propanediol, which can be used for continuous fermentation and production of 1,3-propanediol and is practical and efficient, especially for anaerobic processes. the

为了达成上述目的，本实用新型的解决方案是： In order to achieve the above object, the solution of the utility model is:

一种生产1，3-丙二醇的微生物固定化发酵罐，包括对应扣合的罐体和罐盖，该罐内设有用于装入固定化载体的反应管组，该罐体上设有与该反应管组的进口端和出口端对应连通的进料管和出料管，所述罐盖通过锁扣装置以能开启和盖合的方式活动设在所述罐体上，所述反应管组通过隔板以能取出和装入的方式活动装设在所述罐体内。 A microbial immobilized fermenter for producing 1,3-propanediol, comprising a correspondingly fastened tank body and a tank cover, the tank is provided with a reaction tube group for loading an immobilized carrier, and the tank body is provided with the The inlet end and the outlet end of the reaction tube group are correspondingly connected to the feed pipe and the discharge pipe. The tank cover is movable on the tank body in a manner that can be opened and closed through a locking device. The reaction tube group The tank is movably installed in the tank in a manner that can be taken out and loaded through a partition. the

所述隔板上设有管道孔和多个料液孔，所述反应管组插设在该管道孔内。 A pipe hole and a plurality of feed liquid holes are arranged on the separator, and the reaction tube group is inserted in the pipe hole. the

所述反应管组为两组或两组以上，所述隔板上的所述管道孔也对应为两个或两上以上。 There are two or more groups of reaction tubes, and there are two or more pipe holes on the separator. the

所述锁扣装置包括短轴、强力弹簧、锁扣头和拉手，该短轴通过销轴固定在所述罐体上，该强力弹簧穿在该短轴上，该锁扣头和该拉手固定在罐盖，且该拉手具有与该锁扣头相锁扣的锁眼。 The lock device includes a short shaft, a strong spring, a lock head and a handle, the short shaft is fixed on the tank body through a pin, the strong spring is worn on the short shaft, the lock head and the handle are fixed On the tank cover, and the handle has a keyhole locked with the lock head. the

所述锁扣装置为3-5个。 There are 3-5 locking devices. the

采用上述结构后，本实用新型生产1，3-丙二醇的微生物固定化发酵罐具有以下有益效果：在封闭扣合的罐体和罐盖内设置反应管组，通过控制罐体内的各参数，能为微生物生长提供设定的温度、湿度、培养基、空气、pH值等，以满足物料的混合、灭菌、降温、保温、研制的要求；通过罐体上的安装的进料管和出料管，可以实现连续发酵；通过锁扣装置能够实现罐盖与罐体的锁合功能，开启锁扣装置后打开罐盖，将活动装设在隔板上的反应管组取出，并进行更换或清洗作业，操作起来极为便利；而且其结构紧凑，节约投资成本，尤其适用于好氧或厌氧发酵生产1，3-丙二醇。 After adopting the above structure, the utility model produces 1,3-propanediol microbial immobilized fermenter has the following beneficial effects: a reaction tube group is set in the closed and fastened tank body and the tank cover, and by controlling each parameter in the tank body, it can Provide set temperature, humidity, culture medium, air, pH value, etc. for the growth of microorganisms to meet the requirements of material mixing, sterilization, cooling, heat preservation, and development; tubes, which can realize continuous fermentation; the locking function of the tank cover and the tank body can be realized through the locking device. After the locking device is opened, the tank cover is opened, and the reaction tube group installed on the partition is taken out and replaced or replaced. The cleaning operation is very convenient to operate; moreover, it has a compact structure and saves investment costs, and is especially suitable for producing 1,3-propanediol by aerobic or anaerobic fermentation. the

附图说明Description of drawings

图1为本实用新型生产1，3-丙二醇的微生物固定化发酵罐的结构示意图； Fig. 1 is theutility model production 1, the structural representation of the microbial immobilization fermenter of 3-propanediol;

图2为本实用新型中的反应管组在罐体内的装设示意图； Fig. 2 is the schematic diagram of installation of the reaction tube group in the tank body in the utility model;

图3为本实用新型中的锁扣装置的示意图； Fig. 3 is the schematic diagram of locking device in the utility model;

图4为本实用新型中的隔板的示意图； Fig. 4 is the schematic diagram of dividing plate among the utility model;

图5为本实用新型生产1，3-丙二醇的微生物固定化发酵罐的流程框图。 Fig. 5 is a block flow diagram of the microbial immobilized fermenter for producing 1,3-propanediol in the present invention. the

图中： In the picture:

罐体            1            锁扣装置        11 Tank Body 1Locking Device 11

短轴            111          强力弹簧        112 Short shaft 111Strong spring 112

锁扣头          113          拉手            114 LockHead 113Handle 114

销轴            115          进料管          12 Pin shaft 115Feed pipe 12

出料管          13           隔板            14 Outlet pipe 13Partition 14

管道孔          141          料液孔          142 Pipehole 141 Materialliquid hole 142

罐盖            2            排气管道        21 Tank cover 2exhaust pipe 21

压力计          22           把手            23 Manometer 22Handle 23

反应管组        3            加热装置        4 Reaction Tube Group 3 Heating Device 4

气体供给装置    5            填料供给装置    6 Gas supply device 5 Filling supply device 6

种液供给装置    7            种液            71 Seed solution supply device 7 Seedsolution 71

具体实施方式Detailed ways

为了进一步解释本实用新型的技术方案，下面通过具体实施例来对本实用新型进行详细阐述。 In order to further explain the technical solution of the utility model, the utility model is described in detail through specific examples below. the

如图1至5所示，本实用新型的生产1，3-丙二醇的微生物固定化发酵罐，包括罐体1、罐盖2、反应管组3、加热装置4、气体供给装置5、填料供给装置6和种液供给装置7。 As shown in Figures 1 to 5, the microbial immobilized fermenter for producing 1,3-propanediol of the present utility model includes atank body 1, atank cover 2, areaction tube group 3, a heating device 4, a gas supply device 5, a filler supply Device 6 and seed liquid supply device 7. the

罐体1和罐盖2为复合保温结构，罐体1和罐盖2通过多个锁扣装置11连接。罐体1内设有隔板14和温度探头，罐体1的下部分别设有进料管12和出料管13。罐盖2上设有排气管道21、压力计22和把手23。 Thetank body 1 and thetank cover 2 are of a composite thermal insulation structure, and thetank body 1 and thetank cover 2 are connected by a plurality of lockingdevices 11 . Apartition 14 and a temperature probe are arranged inside thetank body 1 , and afeed pipe 12 and adischarge pipe 13 are respectively provided at the lower part of thetank body 1 . Thetank cover 2 is provided with anexhaust pipe 21 , apressure gauge 22 and ahandle 23 . the

沿罐体1和罐盖2的圆柱面分布3个锁扣装置11，各锁扣装置11分别包括短轴111、强力弹簧112、锁扣头113、拉手114和销轴115。短轴111通过销轴115固定在罐体1上，强力弹簧112穿在短 轴111上，锁扣头113和拉手114固定在罐盖2，拉手114具有与锁扣头113相锁扣的锁眼。锁扣罐盖2时，向下压拉手114并挤压强力弹簧112，使拉手114上的锁眼卡入锁扣头113。打开罐盖2时，先旋转拉动拉手114，随着强力弹簧112的拉起，将强力弹簧112向外侧拉一下，使拉手114与锁扣头113脱离；将其余的2个锁扣装置11开启后，掀开罐盖2，即可对罐体1进行清洗和更换菌种的操作。 Threelocking devices 11 are distributed along the cylindrical surface of thetank body 1 and thetank cover 2 , and each lockingdevice 11 includes ashort shaft 111 , astrong spring 112 , a lockinghead 113 , ahandle 114 and apin 115 . Theshort shaft 111 is fixed on thetank body 1 through thepin shaft 115, thestrong spring 112 is worn on theshort shaft 111, thelock head 113 and thehandle 114 are fixed on thetank cover 2, and thehandle 114 has a lock that is locked with thelock head 113 Eye. When locking thetank lid 2 , press thehandle 114 downward and squeeze thestrong spring 112 , so that the keyhole on thehandle 114 snaps into thelock head 113 . When opening thetank cover 2, first rotate and pull thehandle 114, and with the pulling up of thestrong spring 112, pull thestrong spring 112 outward to disengage thehandle 114 from thelock head 113; open the remaining twolock devices 11 Finally, open thetank cover 2, thetank body 1 can be cleaned and replaced with bacteria. the

反应管组3由多个反应管组成，通过隔板14装设在罐体1内；反应管组3的进料端和出料端分别与罐体1下部进料管12和出料管13的一端对应相连通，进料管12的另一端与种液供给装置7相连通。隔板14上设有多个孔径较大的管道孔141，以及多个孔径较小的料液孔142；这样，多组反应管组3分别穿设在管道孔141内，得以固定装设在罐体1内，料液可以通过料液孔142在罐体1内流通。 Thereaction tube group 3 is composed of a plurality of reaction tubes, which are installed in thetank body 1 through apartition 14; One end of thefeed pipe 12 is communicated with each other correspondingly, and the other end of thefeed pipe 12 is communicated with the seed liquid supply device 7 . Thepartition plate 14 is provided with a plurality of pipe holes 141 with larger apertures and a plurality of feed liquid holes 142 with smaller apertures; in this way, multiple groups ofreaction tube groups 3 are respectively installed in the pipe holes 141, and can be fixedly installed in the pipe holes 141. In thetank body 1 , the feed liquid can circulate in thetank body 1 through thefeed liquid hole 142 . the

加热装置4可采用现有的多种加工结构，例如换热器、电加热方式等，加热装置4设在罐体1外并与种液供给装置7相连接，用以对由种液供给装置7供给至罐体1内的液体进行预热。此外，加热装置4还可在必要时提供对特定的进料液进行高温灭菌。 The heating device 4 can adopt existing multiple processing structures, such as heat exchangers, electric heating methods, etc., and the heating device 4 is arranged outside thetank body 1 and is connected with the seed liquid supply device 7, in order to control the temperature of the seed liquid supply device. 7. The liquid supplied into thetank body 1 is preheated. In addition, the heating device 4 can also provide high-temperature sterilization for specific feed liquids when necessary. the

气体供给装置5主要包括依次连通的气源、前置过滤器、压缩机和气体过滤器，气体供给装置5设在罐体1外，上述气体过滤器通过管道与罐体1底部的进料管12相连通，在厌氧发酵时用以对罐体1内装设的反应管组3吹送氮气驱氧，而且氮气透过反应管组3的管壁进入罐体1内，还可同时实现对罐体1初期的送氮气驱氧和中后期的及时带走菌体在代谢过程中产生的CO₂。此外，该装置也可用于好氧过程的空气输送。 The gas supply device 5 mainly includes a gas source, a pre-filter, a compressor and a gas filter connected in sequence. The gas supply device 5 is arranged outside thetank body 1, and the gas filter passes through the pipeline and the feed pipe at the bottom of thetank body 1. 12 connected, used to blow nitrogen to drive oxygen to thereaction tube group 3 installed in thetank body 1 during anaerobic fermentation, and the nitrogen gas enters thetank body 1 through the tube wall of thereaction tube group 3, and it can also realize the reaction of the tank body at the same time In the early stage ofcell 1, send nitrogen to drive away oxygen, and in the middle and late stages, take away the CO₂ produced by the cell in the metabolic process in time. Furthermore, the unit can also be used for air delivery for aerobic processes.

填料供给装置6和种液供给装置7分别包括进料泵，并分别与罐体1下部进料管12相连通。填料供给装置6对罐体1内装设的反应 管组3供给作为固定菌种的分散型固定化填料(若是模压型填料则通过开启罐盖2进行整体装卸)；种液供给装置7对反应管组3供给发酵液(扩培的种子液以一定比例接种量接种于发酵培养基的发酵液)、种子液(菌种通过各级种子罐扩培的种子液)、培养基(根据微生物营养需求配制适量的各种原料成溶液或悬浮液)、原料液(经适当预处理的原料如碳源的葡萄糖、甘油等)。 The filler supply device 6 and the seed liquid supply device 7 respectively include feed pumps, and communicate with thefeed pipe 12 at the lower part of thetank body 1 respectively. The filler supply device 6 supplies thereaction tube group 3 installed in thetank body 1 with dispersed immobilized filler as a fixed bacterial species (if the molded filler is integrally loaded and unloaded by opening the tank cover 2); the seed liquid supply device 7 pairs thereaction tube Group 3 is supplied with fermentation broth (the expanded seed fluid is inoculated into the fermentation broth with a certain proportion of inoculum), seed fluid (the strains are expanded through seed tanks at all levels), culture medium (according to the nutritional requirements of microorganisms) Preparation of appropriate amount of various raw materials into solutions or suspensions), raw material liquid (raw materials that have been properly pretreated, such as glucose, glycerol, etc. as carbon sources). the

本实用新型适用于多种固定化填料，用作固定菌种的固定化载体多种多样，有高分子载体、无机载体、复合载体和一些新型载体。高分子载体中天然高分子载体有海藻酸钠、壳聚糖及其衍生物、纤维素及其衍生物和珠状琼脂糖凝胶等；合成有机高分子载体有甲基丙烯酸甲酯、聚N-乙烯基-苯丙氨酸微球和丙烯酸共聚物等。无机载体有玻璃纤维膜、分层固体材料、多孔硅介质、硅微芯片和介孔硅酸盐材料等。复合载体有磁性高分子微球、多糖-纤维素和碳纳米复合材料等。一些新型载体有温敏磁性水凝胶和毛细管整体柱等。本实用新型可以适用多种填料操作，只需将填料装填进反应管道即可。目前已试验海棉体、海藻酸钙包埋和PP过滤棉的固定化细胞，其中海绵块可进行一定的切割，而PP过滤棉规格可自制也可采用已量产的净水器常用规格。 The utility model is suitable for a variety of immobilized fillers, and the immobilized carriers used for immobilizing bacterial species are various, including polymer carriers, inorganic carriers, composite carriers and some new carriers. Among the polymer carriers, natural polymer carriers include sodium alginate, chitosan and its derivatives, cellulose and its derivatives, and beaded agarose gel, etc.; synthetic organic polymer carriers include methyl methacrylate, polyN - Vinyl-phenylalanine microspheres and acrylic acid copolymers, etc. Inorganic supports include glass fiber membranes, layered solid materials, porous silicon media, silicon microchips, and mesoporous silicate materials. Composite carriers include magnetic polymer microspheres, polysaccharide-cellulose, and carbon nanocomposites. Some new carriers include temperature-sensitive magnetic hydrogels and capillary monolithic columns. The utility model can be applied to a variety of packing operations, and only needs to fill the packing into the reaction pipeline. At present, the immobilized cells of sponge body, calcium alginate embedding and PP filter cotton have been tested. The sponge block can be cut to a certain extent, and the specifications of PP filter cotton can be self-made or the common specifications of water purifiers that have been mass-produced. the

本实用新型的工作原理如下：开启罐盖2装填反应管组3的填料，若是分散性填料可在发酵过程中，通过填料供给装置6内的固定化填料灭菌后，可单独或与来自种液供给装置7的料液一起通过进料管12装载入反应管组3内；厌氧发酵时，开启气体供给装置5的氮气阀门，对反应管组3进行吹氮气驱氧；将种子培养液通过种液供给装置7加入反应管组3内，调节气体供给装置5的阀门控制适宜的氮气输入气速，保持氮气气路封闭罐体1，恒温培养固定化菌体后，开启 种液供给装置7的进料泵控制适宜进料速率连续发酵；通过出料管13取样，若间歇操作则可通过罐盖2的观察取样口进行间隔取样，测试目标产物浓度控制进料速率并对特定过程决定是否循环处理。 The working principle of the utility model is as follows: open thetank cover 2 and fill the filler of thereaction tube group 3. If the dispersive filler can be sterilized by the immobilized filler in the filler supply device 6 during the fermentation process, it can be used alone or with the filler from the species. The feed liquid of the liquid supply device 7 is loaded into thereaction tube group 3 together through thefeed pipe 12; during anaerobic fermentation, the nitrogen valve of the gas supply device 5 is opened, and thereaction tube group 3 is blown with nitrogen to drive oxygen; The liquid is added into thereaction tube group 3 through the seed liquid supply device 7, and the valve of the gas supply device 5 is adjusted to control the appropriate nitrogen gas input gas velocity, and the nitrogen gas path is kept to close thetank 1. After constant temperature culture and immobilization of bacteria, the seed liquid supply is opened The feeding pump of the device 7 controls the appropriate feeding rate for continuous fermentation; sampling through thedischarge pipe 13, if it is operated intermittently, it can be sampled at intervals through the observation sampling port of thetank cover 2, and the concentration of the target product is tested to control the feeding rate and monitor the specific process. Decide whether to loop. the

初始料液预热即可，发酵培养时，可利用反应热实现保温，同时通过温控微调控制适宜的预热温度，由于温控系统的滞后性及过程产热的影响，实际温度在最适温度的上下3度之间波动，能够满足微生物的生长要求。 The initial material liquid can be preheated. During fermentation, the heat of reaction can be used to keep warm. At the same time, the appropriate preheating temperature can be controlled by fine-tuning the temperature control. The temperature fluctuates between the upper and lower 3 degrees, which can meet the growth requirements of microorganisms. the

发酵过程中，随着细菌的生长，代谢产生的CO₂若在罐内累积，不利发酵生产，同时因部分CO₂溶解降低发酵液的pH值，将增加碱液消耗量；本实用新型通过排气管道21使CO₂及时排出，一方面若是厌氧发酵则控制通入适宜气速的氮气会及时带走菌体在代谢过程中产生的CO₂确保细菌活性和反应速度，好氧发酵则控制通入空气；另一方面若非全程送气，则通过排气管21设置的泄压阀控制罐内的压力，当罐内的压力高于一定值时自动打开排气。 During the fermentation process, with the growth of bacteria, if the_CO2 produced by metabolism accumulates in the tank, it will be unfavorable for fermentation production, and at the same time, the pH value of the fermentation liquid will be reduced due to the dissolution of part of the_CO2 , which will increase the consumption of lye; Theair pipeline 21 makes_CO2 be discharged in time. On the one hand, if it is anaerobic fermentation, the nitrogen gas with an appropriate gas velocity will be controlled to take away the_CO2 produced by the bacteria in the metabolic process in time to ensure bacterial activity and reaction speed. Aerobic fermentation will control On the other hand, if the air is not supplied all the way, the pressure relief valve provided by theexhaust pipe 21 controls the pressure in the tank, and when the pressure in the tank is higher than a certain value, it automatically opens to exhaust.

发酵后，只要通过开启锁扣装置11，打开罐盖2即可对反应组3整体更换固定化载体或对罐体1进行清洗；也可通过种液供给装置7切换进料泵为洗涤泵，由进料管12打入清洗液，然后从出料管13排出。 After fermentation, as long as the lockingdevice 11 is opened and thetank cover 2 is opened, the immobilized carrier can be replaced as a whole in thereaction group 3 or thetank body 1 can be cleaned; the feed pump can also be switched to a washing pump through the seed liquid supply device 7, The cleaning liquid is driven into thefeed pipe 12, and then discharged from thedischarge pipe 13. the

本实用新型与现有的技术对比具有以下优点： Compared with the existing technology, the utility model has the following advantages:

1、本实用新型不需设置搅拌装置，结构紧凑、装卸简单，减少加工成本，节约投资成本； 1. The utility model does not need to be equipped with a stirring device, has a compact structure, is easy to assemble and disassemble, reduces processing costs, and saves investment costs;

2、罐体1和罐盖2为活动式连接，利用锁扣装置11进行锁扣，结构合理易于操作，同时利用隔板14上的孔道来固定反应管组3，易于取换，既方便清洗又提高了工作效率； 2. Thetank body 1 and thetank cover 2 are connected in a movable manner. The lockingdevice 11 is used for locking. The structure is reasonable and easy to operate. At the same time, the holes on thepartition 14 are used to fix thereaction tube group 3, which is easy to replace and easy to clean. Also improved work efficiency;

3、在罐体1内设置气路，排气管道21安装泄压阀，实现对罐内 气体的流通和对压力的限制，同时实现对菌体代谢产生的CO₂、H₂浓度进行有效控制，提高了发酵的质量和效率，而且成本低，易于操作控制，安全可靠； 3. A gas circuit is set in thetank body 1, and a pressure relief valve is installed in theexhaust pipe 21 to realize the circulation of the gas in the tank and the limitation of the pressure, and at the same time realize the effective control of the concentration of CO₂ and H₂ produced by the metabolism of the bacteria , improve the quality and efficiency of fermentation, and low cost, easy to operate and control, safe and reliable;

4、用于预热的加热装置4设在罐体1外，罐体1内采用复合保温结构，整个反应管组3包裹在料液中，提高了热量利用率，能有效节约能量； 4. The heating device 4 for preheating is located outside thetank body 1, and the inside of thetank body 1 adopts a composite heat preservation structure, and the entirereaction tube group 3 is wrapped in the feed liquid, which improves the heat utilization rate and can effectively save energy;

5、物料在同一设备里能够完成混合、厌氧灭菌、培养、发酵、进出料等整个过程，并能动态检测温度、压力和CO₂含量并加以控制，其性价比高，具有推广应用的潜在价值。 5. Materials can complete the entire process of mixing, anaerobic sterilization, cultivation, fermentation, feeding and discharging in the same equipment, and can dynamically detect and control temperature, pressure and_CO2 content. It is cost-effective and has the potential for popularization and application value.

总之，本实用新型适用于固定化技术，可以提高反应速度、缩短生产周期，能有效实现反应产物收率的提高等众多优点；反应器放大效应理想，利用简易的控制系统即可实现微生物固定化发酵生产1，3-丙二醇的生物法过程，必将不断促进固定化生物反应器在大工业生产中的应用。 In a word, the utility model is applicable to the immobilization technology, which can increase the reaction speed, shorten the production cycle, and effectively realize many advantages such as the improvement of the yield of the reaction product; the reactor amplification effect is ideal, and the immobilization of microorganisms can be realized by using a simple control system The biological process of fermenting and producing 1,3-propanediol will surely promote the application of immobilized bioreactors in large-scale industrial production. the

上述实施例和附图并非限定本实用新型的产品形态和式样，任何所属技术领域的普通技术人员对其所做的适当变化或修饰，皆应视为不脱离本实用新型的专利范畴。 The above-mentioned embodiments and accompanying drawings do not limit the product form and style of the present utility model, and any appropriate changes or modifications made by those skilled in the art should be regarded as not departing from the patent scope of the present utility model. the

Claims (5)

1. produce 1 for one kind; The microbial immobilized fermentor tank of ammediol; Comprise corresponding tank body and the cover that fastens; Be provided with the reaction tubes group of the fixation support that is used to pack in this jar; This tank body is provided with and the entrance end of this reaction tubes group and the feed-pipe and the extraction line of the corresponding connection of exit end, and it is characterized in that: said cover is actively installed on the said tank body with the mode that can open and cover through latching system, and said reaction tubes group is installed in the said tank body with the mode activity that can take out and pack into through dividing plate.

2. production 1 as claimed in claim 1, the microbial immobilized fermentor tank of ammediol is characterized in that: said dividing plate is provided with pipe hole and a plurality of feed liquids hole, and said reaction tubes group is plugged in this pipe hole.

3. production 1 as claimed in claim 2, the microbial immobilized fermentor tank of ammediol is characterized in that: said reaction tubes group is two groups or more, more than the said pipe hole on the said dividing plate also corresponds on two or two.

4. production 1 as claimed in claim 1; The microbial immobilized fermentor tank of ammediol; It is characterized in that: said latching system comprises minor axis, brute spring, snap close head and handle, and this minor axis is fixed on the said tank body through bearing pin, and this brute spring is through on this minor axis; This snap close head and this handle are fixed on cover, and this handle has and this snap close head keyhole of snap close mutually.

5. like claim 1 or 4 described productions 1, the microbial immobilized fermentor tank of ammediol is characterized in that: said latching system is 3-5.

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