CN1876801A - Cell detection transducer - Google Patents

Abstract

The invention relates the cell checkout sensing device, comprising small mobile chamber 1, big mobile chamber 2, input connection 11, capillary 5 and 10, and outlet connection 9. The left end of small mobile chamber 1 is connected with the right end of big mobile chamber 2, between them there is hole 8, left end cover 3 is connected with big mobile chamber 2, on the front of left end cover 3 there is sample connector 4 and capillary 5, input connection 11 is connected with left end cover 3, on the right end of small mobile chamber 1 there is outlet connection 9, in the outlet connection 9 there is capillary 10, sample connector 4 is connected with sample tube, the input connection 11 is connected with sheath tube, and the outlet connection 9 is connected with negative pressure chamber. The invention improves the accuracy and reducing the cost.

Description

Cell detection transducer

Technical field

The present invention relates to a kind of cell detection transducer, particularly a kind of cell detection transducer of cellanalyzer.

Background technology

Owing to high-tech develop rapidly, also constantly adopt up-to-date electronics, optics, chemistry and computer technology on the cellanalyzer in recent years, thereby constantly satisfied the requirement that clinical position is analyzed blood cell.Provide convenient and be suitable for, greater functionality and parameter, more accurately, the cellanalyzer of faster speed, be the target of each cellanalyzer production firm.Cellanalyzer adopts multiple detection technique (laser method and impedance method, flow cytometry technology) that blood sample to be measured is carried out hemocyte to carry out differential count, detected result is presented on the screen again and (comprises figure and tabulation), print examining report, finish one-time detection.Cell detection transducer is the key core parts of cellanalyzer, and its design directly has influence on the performance and the technical parameter of cellanalyzer.Cell detection transducer is meant and utilizes Coulter principle, make cell (or other particles) pass through aperture successively at a high speed in single mode, the aperture two ends are connected with the constant current direct current, and when cell passed through aperture, aperture two ends resistance changed, the resistance signal that detects the aperture both sides changes, resistance signal is the pulse that is in proportion with cell volume, signal is further handled, and each parameter is carried out association analysis, just can draw the relevant information of the cell of surveying, play hemanalysis and diagnostic functions.For accuracy and the precision that improves detection, many comparatively high-grade blood analysers have all adopted sheath stream technology at present, sheath stream technology is to utilize principle of hydrodynamics, focus on by detecting aperture with pressure-driven sheath fluid parcel stream of cells, this advanced person's sheath stream technology, can guarantee that in theory cell is positioned at the sheath fluid center and is arranged into row by detecting the center, hole, guaranteeing again that it can not return detects the sensitive area, but it is all very difficult that realization and assurance are got up, and all there is a certain distance in these areas in existing product.

As shown in Figure 4, the cell detection transducer of existing cellanalyzer mainly comprises first flow chamber 41, second flow chamber 42, small structure 43, electrode 44 and 45, sheath fluid joint 47, kapillary 46, sample input adapter 48, discard solution discharge port 49 and reagent input aperture 50.First flow chamber 41 and second flow chamber 42 interconnect, and in the junction small structure 43 that can allow cell pass through are housed.Driving first flow chamber 41 with malleation when detecting cell flows sheath fluid and by aperture 43, from kapillary 46, release sample liquid then, sheath fluid wraps up sample liquid fast by aperture 43 under hydrodynamic action, the voltage signal that detects between the aperture two end electrodes changes, and reaches the purpose that detects cell by the Treatment Analysis to voltage signal.But pressure adopts the frame mode that malleation drives and first flow chamber opens wide in the currently available products technical pattern, complex structure, and pressure-controlling is loaded down with trivial details, and the hydrodynamicpressure fluctuation is big, and test is unstable, the cost height.Cell hangs on the little hole wall especially easily after pressure stops, thereby plug-hole takes place.The mode that first flow chamber opens wide also causes dust to enter flow chamber easily, causes little hole plug.Little hole plug is the total scabrous difficult problem of existing cell detection transducer, also is the emphasis of numerous producers research, and plug-hole has caused the error of cell detection, improves maintenance cost greatly, and has reduced the credit worthiness of product.The second flow chamber sheath fluid refluxes easily, drives cell and returns the detection sensitive area, causes the repeat count of cell.

Summary of the invention

At there being the problems referred to above in the prior art, the object of the present invention is to provide a kind of simple in structure, exquisite composition, the cell detection transducer of all sealings, this device can adopt the negative pressure type of drive, and can reduce cell plug-hole probability greatly, has avoided cell to go back to the sensitive area and has caused repeat count, improve the accuracy of cell detection, can reduce manufacturing and operation cost again simultaneously.

A kind of cell detection transducer comprises small flow chamber 1,big flow chamber 2, sheathfluid input adapter 11, kapillary 5 and 10,outlet connection 9, and wherein, small flow chamber 1 links to each other withbig flow chamber 2, and in the junction jewel hole 8 that can allow cell pass through is housed.In addition, the present invention also is provided with the left end cap 3 that is connected withbig flow chamber 2 left ends, sample joint 4 is equipped with and as anodal kapillary 5 in left end cap 3 front end axle center places, left end cap 3 also is connected to 11 two of sheath fluid input adapters,big flow chamber 2 right-hand members directly link to each other with small flow chamber 1 left end, what the right-hand member of small flow chamber 1 connectedoutlet connection 9 as negative pole arranged, be provided with sample close-fitting in theoutlet connection 9 and reclaim kapillary 10 with it, atbig flow chamber 2 and 1 junction, small flow chamber concentric jewel hole 8, sample joint 4 are housed, sheathfluid input adapter 11 andoutlet connection 9 respectively with the liquid road in the sample pipeline, sheath stream pipeline links to each other with negative pressure chamber.

Further, between describedbig flow chamber 2 and left end cap 3, be provided with tightness system 6, betweenbig flow chamber 2 and small flow chamber 1, be provided with tightness system 7.

Described tightness system 6 and 7 is O RunddichtringO, v-ring, y-shaped ring or gasket.

Described tightness system 6 and 7 is to adopt the material with protection against corrosion and wear-resisting functions to make, and above-mentioned materials comprises polytetrafluoroethylene PTFE, fluoroplastics.

Describedbig flow chamber 2 adopts screw 12 to fixedly connected with small flow chamber 1 and 3 of left end caps.

1 rotational symmetry both sides, described small flow chamber are provided withtransparent observation fenestella 16.

Described small flow chamber 1 adopts the non-conductive transparent material with corrosion proof function to make, and above-mentioned materials comprises synthetic glass PMMA and polycarbonate PC, and described observation fenestella (16) is made one with small flow chamber (1).

Describedbig flow chamber 2 and left end cap 3 adopt the nonconducting polymer materials with high resistivity to make, and above-mentioned materials comprises polysulfones, acrylonitrile-butadiene-styrene copolymer ABS.

Described kapillary 5 materials are stainless material, and external diameter is 1～2mm, and internal diameter is 0.4～0.8mm, and the big end of the cone surface of the exit end of kapillary 5 andbig flow chamber 2 inner chambers 15 is concordant during installation.

Described sheathfluid input adapter 11 adopts acrylonitrile-butadiene-styrene copolymer ABS material to make, external diameter is 1.5～2.5mm, sheathfluid input adapter 11 1 ends are installed in the bottom,big chamber 14 ofbig flow chamber 2, and the other end is installed in the end face or the place, side of left end cap 3, becomes 180 ° of symmetric arrangement.

Described sheathfluid input adapter 11 links to each other by screw thread with left end cap 3 with sample joint 4 or makes one.

Describedoutlet connection 9 adopts the conductive transparent material with corrosion proof function to make, and above-mentioned materials comprises: corrosion-resisting aluminium and titanium alloy.

Describedoutlet connection 9 links to each other by screw thread with small flow chamber 1 or makes one.

The external diameter of described kapillary 10 is 1.5～2.5mm, and internal diameter is 1.0～1.5mm, and the entrance end of kapillary 10 is concordant with the big end 13 of small flow chamber conical surface.

Described kapillary 10 adopts stainless steel or corrosion resistant macromolecular material to make.

Described jewel hole 8 adopts artificial ruby or artificial white stone material to make, and diameter is between 60～100um, and thickness is between 50～100um.

Effect of the present invention is: apparatus structure of the present invention is simple, and is easy to use.The employing negative pressure is a motivating force, symmetric sheath fluid input channel operates steadily sheath fluid, makes sheath fluid parcel sample flow stably pass through aperture under the water power focussing force, the sample input and output all have the kapillary transmission, and finish the back to the aperture cleaning that recoils in each pattern detection.These structures and method and measure, it is loaded down with trivial details to solve positive pressure-controlled well, and test is unstable, and often plug-hole focuses on instability, and great number of issues such as cost height have been avoided cell to go back to the sensitive area and have been caused repeat count.And, adopt totally enclosed structure also to avoid entering of dust, reduced the probability of plug-hole.Therefore, adopt device of the present invention, can improve the accuracy of cell detection greatly, reduced the maintenance and operations cost.

Description of drawings

Fig. 1 is cell detection transducer three-dimensional profile figure of the present invention;

Fig. 2 is a cell detection transducer front view of the present invention;

Fig. 3 is the A-A sectional view of Fig. 2;

Fig. 4 is the structural representation of cell detection transducer in the prior art;

Fig. 5 is a cell detection transducer end view of the present invention;

Fig. 6 is the B-B part sectioned view of Fig. 5.

Embodiment

The present invention is described in further detail below in conjunction with the drawings and specific embodiments.

As Fig. 1, Fig. 2, Fig. 3, Fig. 5 and shown in Figure 6, a kind of cell detection transducer, chief component have small flow chamber 1,big flow chamber 2, left end cap 3, jewel hole 8 etc.Small flow chamber 1 links to each other withbig flow chamber 2,1 rotational symmetry both sides, small flow chamber are provided with transparent observation fenestella 16 (in the present embodiment, observe fenestella 16 and make one) with small flow chamber 1, use in order to installation and maintenance, non-conductive transparent material such as organic glass PMMA and the polycarbonate PC etc. with corrosion proof function are adopted in small flow chamber 1, and the material that adopts in the present embodiment is polycarbonate PC.

Big flow chamber 2 links to each other with left end cap 3, andbig flow chamber 2 and left end cap 3 adopt the nonconducting polymer materials with high resistivity, and as polysulfones, acrylonitrile-butadiene-styrene copolymer ABS etc., what adopt in the present embodiment is polysulfones.

Be equipped with co-axial sample joint 4 and import kapillaries 5 with sample joint 4 close-fitting samples at place, left end cap 3 front end axle center, left end cap 3 and sample joint 4 link to each other by screw thread, kapillary 5 materials are stainless material, external diameter is 1～2mm, internal diameter is 0.4～0.8mm, as the positive pole of cell sensing device, during installation the exit end of kapillary 5 must and greatly the big end of the cone surface offlow chamber 2 inner chambers 13 is concordant.Also be connected to 11 two of sheath fluid input adapters on left end cap 3 circumferential wall, joint 11 adopts acrylonitrile-butadiene-styrene copolymer ABS material to make, and external diameter is 1.5～2.5mm, and the external diameter of presentembodiment center tap 11 is 2mm.

As shown in Figure 6, sheathfluid input adapter 11 1 ends are installed in the bottom,big chamber 14 ofbig flow chamber 2, are communicated with the big chamber ofbig flow chamber 2, and the other end is installed in the place, side of left end cap 3, becomes 180 ° of symmetricarrangement.Outlet connection 9 and small flow chamber 1 link to each other by screw thread, andoutlet connection 9 adopts conductive transparent material such as the making such as corrosion-resisting aluminium and titanium alloy with corrosion proof function, and it is as the negative pole of cell sensing device.Kapillary 10 leakproof fit with it is housed inoutlet connection 9, and kapillary 10 adopts stainless steel to make, and external diameter is 1.5～2.5mm, and internal diameter is 1.0～1.5mm, and in the present embodiment, external diameter is 2.0mm, and internal diameter is 1.2mm.The entrance end of kapillary 10 must be concordant with the big end 15 of small flow chamber conical surface, to reach the sample flow that absorbs fully by jewel hole 8, avoids sample flow to loculus dissipation everywhere, increases the difficulty of cleaning.

Jewel hole 8 is contained between the 1big flow chamber 2 of small flow chamber, and jewel hole 8 adopts artificial ruby to make, and diameter is between 60～100um, and thickness is between 50～100um, and in the present embodiment, the diameter in jewel hole 8 is 80um, and thickness is 75um.

The O RunddichtringO 6 of sealing usefulness is housed betweenbig flow chamber 2 and left end cap 3, betweenbig flow chamber 2 and small flow chamber 1 O RunddichtringO 7 is housed, the O RunddichtringO adopts the material polytetrafluoroethylene PTFE with protection against corrosion and wear-resisting functions to make.Big flow chamber 2 adopts screw 12 to fixedly connected with small flow chamber 1 and 3 of left end caps, and screw material adopts the stainless steel with corrosion proof function to make.

In foregoing invention embodiment, sheathfluid input adapter 11 also can be installed in the end of left end cap 3, becomes 180 ° of symmetric arrangement.

In foregoing invention embodiment, the O RunddichtringO can adopt the sealing-ring of other pattern, as: v-ring, y-shaped ring and gasket etc., material also can adopt fluoroplastics etc.

In foregoing invention embodiment, sheathfluid input adapter 11 and sample joint 4 also can be directly and left end cap 3 make one.In foregoing invention embodiment,outlet connection 9 also can be directly and small flow chamber 1 make one.In foregoing invention embodiment,big flow chamber 2 adopts screw 12 to fixedly connected with small flow chamber 1 and 3 of left end caps, and mode of connection can adopt 3 screws, become 120 ° of distributions, also can adopt 4 screws, become 90 ° of distributions, screw material also can adopt etch-proof alloy material.

In foregoing invention embodiment, jewel hole 8 materials adopt artificial ruby, also can adopt artificial white stone material.

In foregoing invention embodiment, kapillary 10 also can adopt corrosion resistant other macromolecular material.

The use of device of the present invention is as follows:

Before use, the cell sensing device is installed in the shielding box with electro-magnetic screen function, avoids the interference of external electromagnetic signal pair cell test signal.The test cell the first step will be cleaned each flow channel for liquids, mainly isbig flow chamber 2 and small flow chamber 1, in order to avoid have external small-particle such as dust in test process, to stop up jewel hole 8, and these particulate plug-holes all are to be difficult to clean and handle.Outlet connection 9 connects negative pressure chamber, and negative pressure will be stablized in the negative pressure chamber, and fluctuation is little, and size will guarantee that flow rate of liquid is between 6～10m/s in the jewel hole 8.After opening negative pressure, the pipeline that links to each other withsheath fluid joint 11 stably sucks sheath fluid, focus on the back by jewel hole 8, after sheath fluid focusing is stable, with micro-injection pump injecting blood sample, blood sample is flowed out uniformly and stably, after the outflow from kapillary 5, the cell size is quick by jewel hole 8 under the water power focussing force one by one because the focussing force of sheath fluid, blood sample are compressed into by the sheath fluid parcel.Cause the resistance change between two ends positive pole (kapillary 5) negative pole (outlet connection 9) when the aperture of cell by jewel hole 8, detect the resistance change signal between positive pole and the negative pole, signal is handled again, just the energy pair cell is analyzed and is diagnosed.After the sample injection finishes, recoiled in jewel hole 8, can prevent that the molecule in cell and the sheath fluid from stopping up aperture, reduce the dynamics of user's cleaning.The recoil back that finishes is cleaned big flow chamber and small flow chamber, prevents that cell is detained flow chamber, in order to next safety detection, the signal acquisition process of resistance change just exactly pair cell carry out analyzing and diagnosing.

Device of the present invention is not limited to the embodiment described in the embodiment, and those skilled in the art's technical scheme according to the present invention draws other embodiment, belongs to technological innovation scope of the present invention equally.

Claims (16)

1. cell detection transducer, comprise small flow chamber (1), big flow chamber (2), sheath fluid input adapter (11), kapillary (5) and (10), outlet connection (9), wherein, small flow chamber (1) links to each other with big flow chamber (2), and the jewel hole (8) that can allow cell pass through is housed in the junction, it is characterized in that: the present invention also is provided with the left end cap (3) that is connected with big flow chamber (2) left end, sample joint (4) is equipped with and as anodal kapillary (5) in left end cap (3) front end axle center place, left end cap (3) also is connected to (11) two of sheath fluid input adapters, big flow chamber (2) right-hand member directly links to each other with small flow chamber (1) left end, what the right-hand member of small flow chamber (1) connected outlet connection (9) as negative pole arranged, be provided with sample close-fitting in the outlet connection (9) and reclaim kapillary (10) with it, concentric jewel hole (8) is housed, sample joint (4) at big flow chamber (2) and junction, small flow chamber (1), sheath fluid input adapter (11) and outlet connection (9) respectively with the liquid road in the sample pipeline, sheath stream pipeline links to each other with negative pressure chamber.

2. a kind of cell detection transducer as claimed in claim 1 is characterized in that: be provided with tightness system (6) between described big flow chamber (2) and left end cap (3), be provided with tightness system (7) between big flow chamber (2) and small flow chamber (1).

3. a kind of cell detection transducer as claimed in claim 2 is characterized in that: described tightness system (6) and (7) are O RunddichtringO, v-ring, y-shaped ring or gasket.

4. a kind of cell detection transducer as claimed in claim 2 is characterized in that: described tightness system (6) and (7) are to adopt the material with protection against corrosion and wear-resisting functions to make, and above-mentioned materials comprises polytetrafluoroethylene PTFE, fluoroplastics.

5. a kind of cell detection transducer as claimed in claim 1 is characterized in that: adopt screw (12) to fixedly connected between described big flow chamber (2) and small flow chamber (1) and left end cap (3).

6. a kind of cell detection transducer as claimed in claim 1 is characterized in that: rotational symmetry both sides, described small flow chamber (1) are provided with transparent observation fenestella (16).

7. a kind of cell detection transducer as claimed in claim 6, it is characterized in that: described small flow chamber (1) adopts the non-conductive transparent material with corrosion proof function to make, above-mentioned materials comprises synthetic glass PMMA and polycarbonate PC, and described observation fenestella (16) is made one with small flow chamber (1).

8. a kind of cell detection transducer as claimed in claim 1, it is characterized in that: described big flow chamber (2) and left end cap (3) adopt the nonconducting polymer materials with high resistivity to make, and above-mentioned materials comprises polysulfones, acrylonitrile-butadiene-styrene copolymer ABS.

9. as claimed in claim 1-kind of cell detection transducer, it is characterized in that: described kapillary (5) material is a stainless material, external diameter is 1～2mm, and internal diameter is 0.4～0.8mm, and the exit end of kapillary during installation (5) is concordant with the big end of cone surface (15) of big flow chamber (2) inner chamber.

10. a kind of cell detection transducer as claimed in claim 1, it is characterized in that: described sheath fluid input adapter (11) adopts acrylonitrile-butadiene-styrene copolymer ABS material to make, external diameter is 1.5～2.5mm, sheath fluid input adapter (11) one ends are installed in the big bottom, chamber (14) of big flow chamber (2), the other end is installed in the end face or the place, side of left end cap (3), becomes 180 ° of symmetric arrangement.

11. a kind of cell detection transducer as claimed in claim 1 is characterized in that: described sheath fluid input adapter (11) links to each other by screw thread with left end cap (3) with sample joint (4) or makes one.

12. a kind of cell detection transducer as claimed in claim 1 is characterized in that: described outlet connection (9) adopts the conductive transparent material with corrosion proof function to make, and above-mentioned materials comprises: corrosion-resisting aluminium and titanium alloy.

13. a kind of cell detection transducer as claimed in claim 1 is characterized in that: described outlet connection (9) links to each other by screw thread with small flow chamber (1) or makes one.

14. a kind of cell detection transducer as claimed in claim 1 is characterized in that: the external diameter of described kapillary (10) is 1.5～2.5mm, and internal diameter is 1.0～1.5mm, and the big end (13) of the entrance end of kapillary (10) and small flow chamber conical surface is concordant.

15. a kind of cell detection transducer as claimed in claim 10 is characterized in that: described kapillary (10) adopts stainless steel or corrosion resistant macromolecular material to make.

16. a kind of cell detection transducer as claimed in claim 1 is characterized in that: described jewel hole (8) adopts artificial ruby or artificial white stone material to make, and diameter is between 60～100um, and thickness is between 50～100um.

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