CN1736489A - Process for preparing nano-granule of albumin - Google Patents

Abstract

Disclosed is a process for preparing nano-granule of albumin which comprises, charging certain amount of bovine serum albumin into water, adjusting pH with HCl, slowly loading ethanol solution, instilling right amount of glutaric dialdehyde as hardening agent, stirring, stewing 2h at room temperature, decompressing to volatilize residual ethanol, subjecting the solution to centrifugation treatment, finally removing residual albumin and cross linking agent.

Description

The preparation method of albumin nano granular

Technical field

The present invention relates to a kind of preparation method to gene therapy of liver cancer medicine nano-carrier.

Technical background

Primary hepatocarcinoma is one of most popular 10 kinds of malignant tumor in the world, and annual about 38.6 ten thousand people in the whole world die from hepatocarcinoma, and wherein 45% in China.Present routine clinical treatment, the excision rate is low, and the chemotherapy targeting is poor, and toxic and side effects is obvious.In recent years, gene therapy obtains a lot of achievements aspect basic research, however because the defective of viral vector, and liposome easily degrades, factors such as instability make gene therapy be subjected to certain restriction.

Summary of the invention

Purpose of the present invention is that a kind of preparation method with albumin nano granular that targeting is strong, medicine comprises the rate height, drug release rate is controlled will be provided.

The preparation method of albumin nano granular of the present invention is: get a certain amount of bovine serum albumin and add in the entry, adjust the ph value with HCl, slowly pour alcoholic solution to solution into and milky occurs, splash into an amount of sclerosing agent glutaraldehyde, agitation as appropriate at room temperature leaves standstill 2h, after decompression makes remaining ethanol volatilization, gained solution is removed remaining albumin and cross-linking agent by centrifugal treating, promptly obtains albumin nano granular of the present invention.

The albumin nano granular that the present invention obtains has the liver property that significantly becomes to liver, and its Targeting Performance significantly reduces the toxicity of chemotherapeutics, can improve curative effect.Can also improve the antitumous effect of chemotherapeutics, significantly suppress the growth of minimal disease, reduce postoperative recurrence greatly.

Specific implementation method

Specifically introduce the step of a albumin nano granular of preparation below in conjunction with embodiment.

Getting an amount of bovine serum albumin adds in the 100ml distilled water, adjust ph value to 5.5 with 0.1N HCl, slowly pour alcoholic solution into, milky occurs until solution, dropwise splash into the sclerosing agent that concentration is 0.06～12.5 μ g/mg, agitation as appropriate, at room temperature leave standstill 2h, after decompression made remaining ethanol volatilization, gained solution centrifugal 30min when 17000rmp promptly got the product that the present invention obtains to remove remaining albumin and cross-linking agent.

Claims (1)

1, a kind of preparation method of albumin nano granular, making and settlement are equipped with the product that a the present invention obtains, the steps include: to get an amount of bovine serum albumin adds in the 100ml distilled water, adjust ph value to 5.5 with 0.1N HCl, slowly pour alcoholic solution into, milky appears until solution, dropwise splash into the sclerosing agent that concentration is 0.06～12.5 μ g/mg, agitation as appropriate, at room temperature leave standstill 2h, after decompression made remaining ethanol volatilization, gained solution centrifugal 30min when 17000rmp promptly got the product that the present invention obtains to remove remaining albumin and cross-linking agent.