CN1465300A - Method for Degrading Tobacco Nicotine Using Microorganisms - Google Patents

Abstract

Translated fromChinese

一种利用微生物降解烟草烟碱的方法，利用从露天烟垛周围的土壤中分离出的一种能降解烟碱的菌株，该菌株为烟草假单胞菌(PseudomonasNicotina)，它能在0.8％烟碱浓度培养液中旺盛生长，把该菌液按照烟叶重量1％～5％加入到烟草中，烟草水分为10％～50％，发酵4－8h，烟草烟碱降低率达到10％～30％。利用本发明可以适当调整烟叶原料的烟碱含量大小，提高烟叶可用性，也可以分解烟尘中的烟碱，减少其对环境的污染。A method of using microorganisms to degrade tobacco nicotine, using a nicotine-degrading bacterial strain isolated from the soil around the open-air stack, the bacterial strain is Pseudomonas Nicotina, which can be used in 0.8% tobacco It grows vigorously in the alkali concentration culture solution. The bacterial solution is added to the tobacco according to the weight of the tobacco leaves at 1% to 5%. The tobacco moisture is 10% to 50%. Ferment for 4-8 hours, and the tobacco nicotine reduction rate reaches 10% to 30%. . The invention can properly adjust the nicotine content of the tobacco leaf raw material, improve the availability of the tobacco leaf, and can also decompose the nicotine in the smoke dust to reduce its environmental pollution.

Description

Utilize the method for microbial degradation tobacco smoke alkaloid

Technical field:

The present invention relates to utilize the method for microbial degradation tobacco smoke alkaloid, be meant tobacco pseudomonad (Pseudomonas Nicotina) CGMCC NO.0757 especially, the method for degrading nicotine belongs to technical field of microbe application.

Background technology:

Tobacco is the special economic crop, contains large number of biological alkali in the tobacco leaf, and its main component is a nicotine, because nicotine has special physiological function, can cause smoker's smoking desire, and this is the main cause of smoker's smoking.Require the nicotine content cut filler moderate when cigarette composition designs, the too high meeting of nicotine content causes the thorn pungent sense of choking, and nicotine content crosses that low then flue gas is flat.Because the undue pursuit of tobacco planting in recent years high fragrance, high concentration and high-nicotine, the result causes the nicotine content of tobacco leaves of producing in recent years too high, does not meet the cigarette composition designing requirement.The nicotine content that China produces burley tobaccos produces burley tobaccos apparently higher than the U.S., and the burley tobaccos characteristic aroma is not obvious, the smoke bitterness.In addition, the nicotine that contains in the volume of smoke rubbish that cigarette enterprise is toppled over pollutes environment without degradation treatment.

Summary of the invention:

Main purpose of the present invention is to provide a kind of method of utilizing the microbial degradation tobacco smoke alkaloid, can adjust the nicotine content size of raw tobacco material, improves tobacco leaf usability.

Another object of the present invention is to provide a kind of method of utilizing the microbial degradation tobacco smoke alkaloid, can decompose the nicotine in the flue dust, reduce its pollution environment.

The objective of the invention is to be achieved through the following technical solutions:

With the 1%-5% of tobacco pseudomonad liquid according to tobacco leaf weight, join in the tobacco of the moisture 10%-50% of being divided into, fermentation 4-8h can make tobacco leaf nicotine reduce.

The preparation method of described tobacco pseudomonad is: the microbial strains of isolating degrading nicotine from open-air cigarette buttress soil on every side, carry out separation and purification and the experiment of tolerance nicotine ability then, find the tobacco pseudomonad of energy vigorous growth in 0.8% nicotinic density nutrient solution.

Described tobacco pseudomonad cultural method is:

Beef extract 0.3-0.8%, peptone 1-2%, NaCl 0.3-0.8%, water 98ml mix, and regulate the pH value to 6.0-7.0, the preparation 100ml 250ml triangular flask of packing into, and sterilization, inoculation in the time of 33-39 ℃ is changeed in the shaking table at 100-150 and to be cultivated 16-32h.

Described tobacco pseudomonad is cultivated method for optimizing:

Beef extract 0.5%, peptone 1%, NaCl 0.5%, water 98ml mix, and regulate pH value to 6.4, the preparation 100ml 250ml triangular flask of packing into, and sterilization, inoculation in the time of 37 ℃ is cultivated 24h in 115 commentaries on classics shaking tables.

The present invention can reach following effect:

Utilize microorganism to come degrading nicotine, can suitably adjust the nicotine content size of raw tobacco material, improve tobacco leaf usability, also can decompose the nicotine in the flue dust, reduce its pollution environment.

The specific embodiment:

Below in conjunction with specific embodiment the present invention is elaborated:

Tobacco pseudomonad (Pseudomonas Nicotina) is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 2nd, 2002, and it abbreviates CGMCC as, and deposit number is CGMCC NO.0757, and this culture of strains method is:

Beef extract 0.5%, peptone 1%, NaCl 0.5%, water 98ml mix, and regulate pH value to 6.4, the preparation 100ml 250ml triangular flask of packing into, and sterilization, inoculation in the time of 37 ℃ is cultivated 24h in 115 commentaries on classics shaking tables.

Get 100g Yunnan Flue-cured Tobacco B₂F, 3ml joins in the tobacco leaf with spray-on process with bacterium liquid, under different moisture content in leaves conditions, in room temperature fermentation certain hour, measures the pipe tobacco nicotine content respectively and changes result such as following table:

Processing time moisture content in leaves nicotine rate of change h % %
	???????40??????????10???????????-12 ???????60??????????30???????????-20 ???????80??????????50???????????-38

Through smokeing panel test, it is pure and mild that flue gas becomes, and excitant obviously reduces, and pungent assorted flavor is eliminated substantially, and overall quality is improved.

Embodiment 1:

Get 100g Yunnan Flue-cured Tobacco B₂F, tobacco moisture are 10%, and spray-on process adds 5ml bacterium liquid, and bacterial concentration is 10⁹Individual/ml, be 65% o'clock at 37 ℃, relative humidity, handle 4h after, nicotine content reduces by 11%.

Embodiment 2:

Get 100g Yunnan Flue-cured Tobacco B₂F, tobacco moisture are 19%, and spray-on process adds 3ml bacterium liquid, and bacterial concentration is 10⁹Individual/ml, be 65% o'clock at 37 ℃, relative humidity, handle 6h after, nicotine content reduces by 23%.

Embodiment 3:

Get 100g Yunnan Flue-cured Tobacco B₂F, tobacco moisture are 50%, and spray-on process adds 1ml bacterium liquid, and bacterial concentration is 10⁹Individual/ml, be 65% o'clock at 37 ℃, relative humidity, handle 8h after, nicotine content reduces by 38%.

It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.

Claims (4)

Translated fromChinese

1、一种利用微生物降解烟草烟碱的方法，其特征在于：将烟草假单胞菌液按照烟叶重量的1％-5％，加入到含水分为10％-50％的烟草中，发酵4-8h，可使烟叶烟碱降低。1, a kind of method utilizing microorganism to degrade tobacco nicotine, it is characterized in that: Pseudomonas tobacco liquid is added in the tobacco that moisture content is 10%-50% according to the 1%-5% of tobacco leaf weight, ferments 4 -8h, can reduce the nicotine in tobacco leaves.2、根据权利要求1所述的利用微生物降解烟草烟碱的方法，其特征在于：所述的烟草假单胞菌是这样获得的：从露天烟垛周围的土壤中分离出降解烟碱的微生物菌株，然后进行分离纯化和耐受烟碱能力实验，找到在0.8％烟碱浓度培养液中能旺盛生长的烟草假单胞菌。2. The method for using microorganisms to degrade tobacco nicotine according to claim 1, characterized in that: said Pseudomonas tobacco is obtained by isolating the nicotine-degrading microorganisms from the soil around the open-air tobacco stacks The strains were then subjected to separation and purification and a nicotine tolerance test to find the Pseudomonas nicotiana that can grow vigorously in the 0.8% nicotine concentration culture solution.3、根据权利要求1所述的利用微生物降解烟草烟碱的方法，其特征在于：所述的烟草假单胞菌培养方法为：3. The method for using microorganisms to degrade tobacco nicotine according to claim 1, characterized in that: the method for culturing Pseudomonas tobacco is:牛肉膏0.3-0.8％、蛋白胨1-2％、NaCl 0.3-0.8％、水98ml混合，调节pH值至6.0-7.0，配制100ml装入250ml三角瓶，灭菌，在33-39℃时接种，在100-150转摇床中培养16-32h。Mix beef extract 0.3-0.8%, peptone 1-2%, NaCl 0.3-0.8%, and water 98ml, adjust the pH value to 6.0-7.0, prepare 100ml into a 250ml Erlenmeyer flask, sterilize, inoculate at 33-39°C, Cultivate for 16-32h in a shaker at 100-150 rpm.4、根据权利要求3所述的利用微生物降解烟草烟碱的方法，其特征在于：所述的烟草假单胞菌培养优选方法为：4. The method for using microorganisms to degrade tobacco nicotine according to claim 3, characterized in that: the preferred method for culturing Pseudomonas tobacco is:牛肉膏0.5％、蛋白胨1％、NaCl 0.5％、水98ml混合，调节pH值至6.4，配制100ml装入250ml三角瓶，灭菌，在37℃时接种，在115转摇床中培养24h。Mix 0.5% beef extract, 1% peptone, 0.5% NaCl, and 98ml water, adjust the pH value to 6.4, prepare 100ml and put it into a 250ml Erlenmeyer flask, sterilize, inoculate at 37°C, and incubate in a shaker at 115 rpm for 24h.