Signal acquisition, analysis and real-time interaction device of adaptive microneedle sensorTechnical Field
The invention relates to the field of biomedical engineering, in particular to a device for collecting, analyzing and interacting a sensing microneedle signal in real time.
Background
Blood analysis is the most widely used biochemical index detection mode in clinical application at present. However, the blood is limited by the inability to collect continuously, and the blood sampling process can cause pain and psychological stress to the patient, limiting its application in real-time, continuous, long-term monitoring of important health indicators, especially in home detection scenarios. In recent years, the subcutaneous interstitial fluid has been studied to be similar to blood plasma in composition and is a biological fluid of great interest. Meanwhile, the biosensor developed based on interstitial fluid has important clinical application value.
The microneedle is a novel biosensor, can easily penetrate through the skin layer to reach the dermis layer and detect interstitial fluid, has the advantages of painless, portability, wearability and the like, and can realize the accurate detection of various types of biomarkers in the interstitial fluid, including electrolytes, small molecules, nucleic acid, protein and the like.
In order to realize intelligent integration of wearable microneedles, a lightweight signal acquisition, analysis and real-time data interaction device needs to be adapted, and signals acquired by the microneedle sensor are captured, transmitted, analyzed and presented in real time, so that the microneedle sensor is promoted to be applied to clinical and daily health monitoring.
Disclosure of Invention
The invention aims to provide a signal acquisition, analysis and real-time data interaction device which is adaptive to a microneedle sensor, and can transmit, present and visually analyze biological signals acquired by the device.
The technical scheme of the invention is as follows:
a signal acquisition, analysis and real-time interaction device adapting to a microneedle sensor comprises a signal acquisition-amplification module, a signal processing module, a signal sending module, a power management module, a program burning module, a mechanical structure module, a microneedle interface and a mobile terminal.
(1) The signal acquisition-amplification module is responsible for receiving electrochemical signals acquired by the microneedle sensor and properly amplifying the electrochemical signals, so that the accuracy of signal reception is ensured;
preferably, the signal acquisition-amplification module can adopt an electrochemical analog front-end chip or an integrated operational amplification chip.
Preferably, the signal amplification module can be expanded into multiple channels for receiving multiple signals, so that the detection of multiple markers is realized.
(2) The signal processing module is used for receiving the amplified electrical signals and converting the electrical signals into digital signals;
preferably, the signal amplification processing module can be selected from SCM such as ESP32-C3-MINI, arduino, MSP 430.
(3) The signal sending module transmits the digital signal to the intelligent mobile terminal in a wireless communication mode;
Preferably, the wireless communication mode includes, but is not limited to, bluetooth, WIFI, NFC, etc.
(4) The power management module is responsible for ensuring normal power supply of each electronic element on the circuit board;
preferably, the power supply chip may select various power management chips such as TPS7a8300, IP5306, and the like.
(5) The program burning module is used for realizing the burning and modification of a control program of the singlechip system and guaranteeing the expansibility of the system;
Preferably, the burning chip can select CH343P, CH and the like, and the singlechip is connected with the outside by using A TYPE-C interface, A USB-A interface, A pin header or bus header and the like, so that the program burning and modification of the singlechip are performed, and the expansibility of the singlechip is ensured.
(6) The mechanical structure module provides mechanical support for the circuit board, and ensures the equipment wearability;
Preferably, the mechanical structure of the invention can be constructed by means of 3D printing, injection moulding, die casting, laser cutting, etc.
Preferably, the mechanical structure material comprises resin, aluminum alloy, titanium alloy, nylon, polypropylene and other materials.
Preferably, the mechanical structure of the present invention may be wristband, bracelet or other ergonomic design, so that the device can be securely worn on the body part of the user, providing greater comfort and convenience.
(7) The microneedle interface is used for connecting the device with the sensing microneedle;
Preferably, the micro needle interface includes a pin header, a flat cable, a connector, an FPC (flexible printed circuit), a ZIF (zero insertion force) connector, and the like.
(8) The intelligent mobile terminal comprises a data receiving module, a data storage module and a data display module which are respectively used for receiving, storing and presenting the microneedle sensing data.
Preferably, the mobile terminal of the present invention includes, but is not limited to, a WeChat applet, an Android application, an iOS application, a mobile Web application, etc., and the data display mode may be any one or more of a dot, a line, a column, a number, and an image.
As described above, the invention provides a signal acquisition and analysis and real-time interaction and presentation device which is adapted to the microneedle sensor, and can be directly coupled with the microneedle sensor so as to realize quantitative detection and visual analysis of target molecules.
Drawings
FIG. 1 is a schematic diagram of a system according to embodiment 1 of the present invention.
Fig. 2 is a circuit diagram of a sensing microneedle signal acquisition and receiving device according to embodiment 1 of the present invention.
In the figure, 1 is a signal processing module and a signal sending module, processes amplified electric signals through a singlechip and transmits processed data to an intelligent mobile terminal through Bluetooth, 2 is a program burning module for program burning and control of a singlechip system, 3 is a power management module for providing stable power supply for an integrated operational amplification chip and the singlechip, and 4 is a signal acquisition amplification-module for acquiring and amplifying signals of a sensing microneedle.
Fig. 3 is a mechanical structure design diagram of embodiment 1 of the present invention.
In the figure, 1 is an upper cover for closing a circuit board and guaranteeing the stability and safety of internal elements, 2 is a bottom plate for bearing and supporting the circuit board and guaranteeing the stable installation of the circuit board, 3 is an electrode connection reserved port for connecting a microneedle sensor and realizing the electric connection between an electrode and the sensor, 4 is a circuit board button reserved position for arranging a power switch button so as to facilitate the switching of control equipment, 5 is a TYPE-C slot port for programming and modifying, provides a convenient interface for data transmission and control with external equipment, 6 is a Bluetooth reserved position for guaranteeing the stable installation and signal transmission of a Bluetooth module and guaranteeing the high-efficiency and stability of wireless communication, and 7 is an electrode lower part reserved position for arranging a related connecting component at the lower part of the electrode and guaranteeing the connection integrity of the electrode and other devices.
Fig. 4 is a physical diagram of a sensing microneedle signal acquisition and receiving device constructed in embodiment 1 of the present invention.
Fig. 5 is a flowchart of an analysis and presentation apparatus for an intelligent mobile terminal according to embodiment 1 of the present invention.
Fig. 6 is a program interface of the intelligent mobile terminal according to embodiment 1 of the present invention.
Fig. 7 is a 3D schematic diagram of a sensing microneedle signal acquisition and receiving device according to embodiment 2 of the present invention.
FIG. 8 is test data of example 2 of the present invention.
Detailed Description
The technical solution of the present application will be further illustrated by examples below with reference to the accompanying drawings, but the scope of the present application is not limited by the specific conditions of these examples.
Example 1:
In this embodiment, a sensing microneedle signal acquisition and receiving device is constructed, and real-time analysis is performed on sensing data, which specifically includes the following steps:
(1) A schematic of the device is shown in fig. 1. The sensing data of the micro needle is amplified and digital-to-analog converted after being acquired and then is transmitted to the intelligent mobile terminal in a wireless mode, and therefore real-time presentation and interaction of the data are achieved.
(2) The circuit design responsible for the signal acquisition, amplification and transmission module is shown in fig. 2, and the mechanical structure design is shown in fig. 3. The mechanical structure is designed in 3D design software, and the preparation is completed by using a 3D printing technology. Subsequently, the circuit board and the mechanical structure are assembled to form a physical diagram as shown in fig. 4.
(3) The analysis flow of the microneedle sensor data is shown in fig. 5, and the intelligent mobile terminal interface (shown in fig. 6) is developed on the basis of the data processing logic in the WeChat applet development platform. Based on the WeChat applet development platform, writing data real-time presentation and interactive program, developing the user interactive interface shown in FIG. 5 according to the program flow shown in FIG. 4.
(4) And (5) signal acquisition and processing. And connecting the sensing microneedle with the microneedle sensor signal acquisition, analysis and interaction device through a physical interface, and using the smart phone as an intelligent mobile terminal to present real-time acquired sensing data.
Example 2:
In this embodiment, a sensing microneedle signal acquisition and receiving device is constructed, and verification and real-time interaction are performed on sensing data, which specifically includes the following steps:
(1) A 3D schematic of the device is shown in fig. 7. The integrated electrochemical chip is adopted to build a sensing microneedle signal acquisition and receiving module, and microneedle sensing data are amplified and digital-to-analog converted after being acquired and transmitted in a wireless communication mode.
(2) The microneedle sensor is connected with the signal acquisition, analysis and interaction device, and the intelligent mobile terminal is used as an intelligent mobile phone to display real-time sensing data. The microneedle sensor may detect an electrochemical signal in interstitial fluid. The connecting process comprises the step of physically connecting the microneedle sensor with the signal acquisition module through an electrode interface, so that stable transmission of signals is ensured. The data is wirelessly transmitted to the intelligent mobile phone in a Bluetooth mode, so that remote monitoring and real-time analysis are realized.
(3) The electrochemical signal in the interstitial fluid is detected by using the device, and the result of real-time data is shown in figure 8, so that the device has extremely high linearity and practical application value.