Disclosure of Invention
Based on the above circumstances, the invention aims to provide a bactericidal composition and a preparation thereof, which are mainly used for preventing and treating plant fungal diseases, wherein the composition or the preparation thereof can enhance the drug effect, reduce the dosage, have obvious synergistic effect under a certain mass ratio, and can prolong the duration and delay the development of drug resistance.
In order to achieve the purpose, the technical scheme is that the bactericidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound shown in a formula (I), and the chemical structural formula is as follows:
The active ingredient B is any one of Feneptamidoquin, fenopyramid;
Further, the mass ratio of the active ingredient A to the active ingredient B is 1:36-20:1, or any value between the above values;
Further, the mass ratio of the active ingredient A to the active ingredient B is 1:24-20:1;
further, the mass ratio of the compound of the formula (I) to Feneptamidoquin is 1:20-18:1;
Further, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:20, 1:9, 1:3, 5:9, 9:5, 9:1, 18:1;
Further, the mass ratio of the compound of the formula (I) to Feneptamidoquin is 1:9-9:1;
Further, the mass ratio of the compound of formula (I) to Feneptamidoquin is 1:9, 1:3, 5:9, 9:5, 9:1;
Further, the mass ratio of the compound of the formula (I) to Fenopyramid is 1:24-20:1;
Further, the mass ratio of the compound of formula (I) to Fenopyramid is 1:24, 1:12, 1:5, 1:2, 3:1, 6:1, 20:1;
Further, the mass ratio of the compound of the formula (I) to Fenopyramid is 1:12-3:1;
Further, the mass ratio of the compound of the formula (I) to Fenopyramid is 1:12, 1:5, 1:2, 3:1;
further, the sum of the contents of the active component A and the active component B in the sterilization composition is 1-90 wt percent based on 100wt percent of the total weight of the sterilization composition;
further, the sum of the contents of the active ingredient A and the active ingredient B in the sterilization composition is preferably 5-80 wt% based on 100wt% of the total weight of the sterilization composition;
Further, the bactericidal composition comprises an agriculturally acceptable auxiliary ingredient in addition to the active ingredient, wherein the auxiliary ingredient is selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers;
Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl sodium succinate, alpha olefin sulfonate, alkylphenol ethoxylate, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, chinese honeylocust fruit powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrating agent F, and/or
Further, the dispersant is selected from one or more of lignosulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerin fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer, and/or
Further, the emulsifier is selected from one or more of calcium dodecyl benzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate, and/or
Further, the thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black, and/or
Further, the disintegrating agent is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid, and/or
Further, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts, and/or
Further, the defoamer is selected from one or more of C10-C20 saturated fatty acid compounds, silicone oil, silicone compounds, C8-C10 fatty alcohol, and/or
Further, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent oil, vegetable oil (soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives and water, and/or
Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, pinocembrane and 1, 2-benzisothiazolin-3-one, and/or
Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2, 6-di-tert-butyl-p-methylphenol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, talcum powder, montmorillonite and starch, and/or
Further, the synergist is selected from synergistic phosphorus, synergistic ether, and/or
Further, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.
Further, the dosage form of the bactericidal composition is selected from a solid preparation and/or a liquid preparation and/or a seed treatment preparation;
The solid preparation comprises powder, granules, balls, tablets, strips, wettable powder, oil dispersion powder, emulsion powder, water dispersible granules, emulsion granules, water dispersible tablets, soluble powder, soluble tablets or soluble granules;
The liquid preparation comprises a soluble agent, an oil agent, a spreading oil agent, an emulsifiable concentrate, a latex, a dispersible agent, a paste, an aqueous emulsion, an oil emulsion, a microemulsion, a lipid agent, a suspending agent, a microcapsule suspending agent, an oil suspending agent, a dispersible oil suspending agent, a suspending emulsion, a microcapsule suspension-suspending agent, a microcapsule suspension-aqueous emulsion or a microcapsule suspension-suspending emulsion;
Further, the powder is a free-flowing powder preparation which is suitable for spraying or spreading and contains an active ingredient;
further, the granule is a granular preparation with a certain particle size range and free flowing active ingredients;
further, the spherical agent is a spherical agent (generally with the diameter larger than 6 mm) containing an active ingredient;
further, the tablet is a tablet formulation having a shape and size containing an active ingredient (typically having two planar or convex surfaces with a spacing of less than a diameter);
Further, the stick is a stick or bar-like preparation (typically a few centimeters long, a few millimeters width/diameter, i.e., a length greater than a diameter/width) containing an active ingredient;
further, the wettable powder is a powdery preparation in which the active ingredients are dispersed into suspension in water;
further, the oil dispersion powder is a powder preparation in which an active ingredient is dispersed into a suspension in an organic solvent;
Further, the emulsion powder is a powdery preparation of which the active ingredients are dissolved by an organic solvent and are wrapped in soluble or insoluble inert ingredients, and the active ingredients are dispersed in water to form oil-in-water emulsion;
Further, the water dispersible granule is a granular preparation which disintegrates in water and the effective components are dispersed into suspension;
further, the emulsion granule is a granular preparation of which the active ingredient is dissolved by an organic solvent and is wrapped in soluble or insoluble inert ingredients, and the active ingredient is dispersed in water to form oil-in-water emulsion;
Further, the water dispersible tablet is a tablet preparation which disintegrates in water and the active ingredient is dispersed into suspension;
Further, the soluble powder is a powdery preparation of which the active ingredient forms a true solution in water, and can contain inert ingredients which are insoluble in water;
further, the soluble granule is a granular preparation of which the effective component forms a true solution in water, and can contain an inert component which is insoluble in water;
further, the soluble tablet is a tablet preparation in which the active ingredient forms a true solution in water, and may contain an inert ingredient which is insoluble in water;
Further, the soluble agent is a liquid preparation which is diluted into transparent or semitransparent containing active ingredients by water, and can contain inert ingredients which are insoluble in water;
Further, the soluble agent is a colloidal preparation which is diluted into true solution by water and contains active ingredients;
Further, the oil is a liquid preparation which is diluted (or not diluted) by an organic solvent to be homogeneous and contains active ingredients;
further, the film spreading oiling agent is an oiling agent which automatically diffuses into an oil film on the water surface and contains active ingredients;
further, the emulsifiable concentrate is a homogeneous liquid preparation which is diluted and dispersed into emulsion by water and contains active ingredients;
Further, the emulsion is a latex preparation which is diluted and dispersed into emulsion by water and contains active ingredients;
Further, the dispersible agent is a homogeneous liquid preparation which is diluted and dispersed into suspension containing the active ingredients by water;
Furthermore, the ointment is a water-based ointment preparation containing active ingredients and capable of forming a film, and is generally used directly;
Further, the aqueous emulsion is an emulsion liquid preparation formed by the active ingredient (or organic solution thereof) in water;
Further, the oil emulsion is an emulsion liquid preparation formed by the effective component (or aqueous solution thereof) in oil;
further, the microemulsion is a microemulsion liquid preparation with the active ingredient being transparent or semitransparent in water, and is used directly or after being diluted by water;
Further, the fat agent is an oil or fat-based viscous preparation containing an active ingredient, and is generally used directly;
further, the suspending agent is a stable suspension liquid preparation prepared by dispersing the active ingredient in water in solid particles, and is generally diluted by water;
Further, the microcapsule suspending agent is a stable suspension liquid preparation formed by dispersing microcapsules containing active ingredients in liquid;
Further, the oil suspending agent is a stable suspension liquid preparation prepared by dispersing solid particles as an active ingredient in liquid, and is generally diluted by an organic solvent;
Further, the dispersible oil suspending agent is a stable suspension liquid preparation prepared by dispersing solid particles of an active ingredient in a non-aqueous medium, and is generally diluted with water for use;
further, the suspoemulsion is a heterogeneous liquid preparation in which the active ingredients are stably dispersed in a continuous water phase in the form of solid particles and water-insoluble tiny liquid drops;
Further, the microcapsule suspension-suspending agent is a stable suspension liquid preparation prepared by dispersing the effective components in water by using microcapsules and solid particles;
Further, the microcapsule suspension-water emulsion is a heterogeneous liquid preparation which is formed by stably dispersing the effective component in a continuous water phase in a microcapsule and micro droplet form;
further, the microcapsule suspension-suspension emulsion is a heterogeneous liquid preparation with active ingredients stably dispersed in a continuous water phase in the forms of microcapsules, solid particles and tiny liquid drops;
Further, the bactericidal composition can be prepared into a preparation formulation which is acceptable in pesticide, wherein the preparation formulation is microemulsion, aqueous emulsion, suspending agent, dispersible oil suspending agent, solution, emulsifiable concentrate, suspending agent, microcapsule suspending agent, water dispersible granule, wettable powder, granule, seed treatment suspending agent and seed treatment dry powder;
Further, the preparation formulation is microemulsion, emulsifiable concentrate, suspending agent, aqueous emulsion, water dispersible granule, wettable powder and seed treatment suspending agent.
The invention also discloses application of the bactericidal composition in preventing and controlling plant diseases;
further, the plant disease is a fungal or bacterial caused plant disease;
further, the plant disease is a fungal-caused plant disease;
Further, the plant disease caused by the fungus is wheat leaf rust;
Wheat leaf rust is a main disease in the growth process of wheat, has the characteristics of wide distribution range, quick transmission, large harm and the like, and is one of main factors causing loss of wheat yield, quality reduction and low benefit. Wheat leaf rust is distributed throughout China, and the occurrence degree of the wheat leaf rust tends to be increased year by year.
The invention has the following beneficial effects:
1) The bactericidal composition increases bactericidal activity, has a synergistic effect on targets in a certain mass ratio, and has the effects of reducing the use amount of pesticides, reducing the use cost and relieving the environmental load;
2) The bactericidal composition is safe and efficient, is safe to crops, non-target organisms, beneficial organisms and natural enemies, and has the effects of increasing yield and protecting income.
Detailed Description
The present invention will be described in more detail with reference to the following examples, but the present invention can be embodied in various forms and should not be construed as being limited to the embodiments set forth herein.
Indoor bioassay of wheat leaf rust
The test basis is that the 15 th part of the bactericide refers to the standard pesticide indoor biological assay test criteria of the agricultural industry of the people's republic of China, and the potting method NY/T1156.15-2008 for preventing and treating wheat leaf rust disease is adopted.
Selecting leaf rust-sensitive wheat variety Luyuan 502 pot culture, sowing 20 seeds in each pot, selecting 10 plants after emergence, growing to 2 leaves and 1 heart period, numbering for standby.
Test targets wheat leaf rust (Puccinia recondita Rob. Ex Desm. F. Sp. Tritici erikss. Et Henn.) the strain was obtained from Shenyang-neutralization agrochemicals research and development Co.
The spore suspension is prepared by washing fresh rust fungus summer spores generated in 24h on the disease-causing leaves with 0.1% Tween-80 water solution, and filtering with 2-4 layers of gauze to prepare a suspension with the concentration of 1X 105 spores/mL for later use.
The preparation of the medicament comprises the steps of dissolving a test medicament with acetone, diluting with 0.1% Tween 80 aqueous solution, designing different proportions according to the purpose of mixing and the activity of the medicament, and preparing each group of single medicament and each group of mixed medicament into a required series of mass concentrations according to an equal proportion method.
And (3) medicament treatment, namely uniformly spraying the liquid medicament on leaf surfaces until the leaf surfaces are completely wet, and naturally airing the liquid medicament for later use. The test was run with no drug-containing treatment as a blank. Each concentration treatment was repeated 4 times, 1 pot per repetition.
Inoculation and cultivation, namely spraying and inoculating the spore suspension. After wheat seedlings are inoculated, the wheat seedlings are subjected to dark moisturizing culture at 20 ℃ for more than 12 hours, the optimal temperature in the moisturizing stage is 15-20 ℃, and then the wheat seedlings are subjected to culture at 18-22 ℃ under the condition that the illumination ratio L is D=12:12 hours.
And (3) data investigation, namely grading investigation of the disease conditions of each treatment when the disease rate of the blank control reaches more than 80%.
The grading method comprises the following steps:
Grade 0, no spore stack;
the spore accumulation accounts for less than 5 percent of the whole leaf area;
3, the spore pile accounts for 5% -10% of the whole leaf area;
5, the spore pile accounts for 10% -25% of the whole leaf area;
the level 7 is that the spore pile accounts for 25% -50% of the whole leaf area;
and 9, the spore pile accounts for more than 50% of the whole leaf area.
Data statistics and analysis:
The disease index is calculated as follows.
Wherein:
X-disease index;
ni -leaf numbers of each stage;
i—relative grade value;
n-total leaf number was investigated.
The control effect is calculated according to the following formula.
Wherein:
P, the prevention and treatment effect, the unit is;
CK-blank disease index;
PT-agent treatment index.
And evaluating the synergistic effect of the mixed medicament according to a grand cloud Pei co-toxicity coefficient method (CTC) by referring to a biological standard method, wherein CTC is equal to or less than 80 and is antagonistic, CTC is equal to or less than 120 and is additive, and CTC is equal to or more than 120 and is synergistic.
Co-toxicity coefficient (CTC) is calculated as follows.
Wherein:
Ati—the measured virulence index of the mixture;
S-EC50 of standard agent in milligrams per liter (mg/L);
M-EC50 of the mixture in milligrams per liter (mg/L).
TTI=TIA×PA+TIB×PB
Wherein:
TTI-theoretical toxicity index of the mixture;
TIA toxicity index of the A agent;
The percentage content of the PA -A medicament in the mixture is expressed as percentage (%);
TIB toxicity index of the B agent;
The percentage of the PB -B medicament in the mixture is expressed as percentage (%).
Wherein:
Ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
And calculating test results by adopting DPS data processing software, and respectively solving a toxicity regression equation, EC50 and 95% confidence limits of the single test agent and the mixed agents with different proportions.
Results of indoor bioactivity assay:
The results in Table 1 show that the compound of formula (I) has a wheat leaf rust control EC50 of 0.2407mg/L and feneptamidoquin wheat leaf rust control EC50 of 0.4519mg/L. (I) The compound and feneptamidoquin are mixed in a mass ratio of 1:20-18:1 to show a synergistic effect, wherein when the mass ratio is 1:20-9:1, the co-toxicity coefficient is more than 150, and the synergistic effect is obvious. The maximum co-toxicity coefficient value of the compound shown in the formula (I) and feneptamidoquin =5:9 is 296.362, and EC50 is 0.1161mg/L.
Table 1 results of measuring synergistic effects of Compounds of formula (I) and feneptamidoquin in different proportions on wheat leaf rust
The results in Table 2 show that fenopyramid controls wheat leaf rust EC50 at 0.6061mg/L. (I) The compound and fenopyramid are mixed in the mass ratio of 1:36-20:1, the co-toxicity coefficient is more than 80, the additive or synergistic effect is shown, and when the mass ratio is 1:24-20:1, the co-toxicity coefficient is more than 120, the synergistic effect is shown. The maximum co-toxicity coefficient value of the compound shown in the formula (I) and fenopyramid =1:2 is 212.040, EC50 is 0.1905mg/L, and the synergistic effect is obvious.
TABLE 2 synergistic effect of Compounds of formula (I) and fenopyramid in different ratios on wheat leaf rust
Formulation examples
Preparation example 1:
42% of a compound of formula (I), feneptamidoquin wettable powder (15:27)
15% Of a compound of the formula (I), feneptamidoquin% of a polycarboxylic acid sodium salt, 5% of a dispersant NNO5%, 5% of polyoxyethylene ether (NV 1420), 3.5% of sodium dodecyl sulfate, 5% of bentonite and the balance of kaolin;
The preparation method comprises the steps of mixing the active ingredients, other functional auxiliary agents and the filler according to the formula proportion, uniformly stirring in a stirring kettle, and carrying out multiple crushing and uniform mixing through a jet mill to obtain the wettable powder of the composition.
Preparation example 2:
22% of a compound of formula (I), feneptamidoquin suspension (8:14)
The formulation formula comprises 8% of a compound of formula (I), feneptamidoquin%, 4% of polyether, 3% of phenethyl phenol polyether phosphate, 3% of calcium sulfonyl succinate (EP 60P), 1.5% of polycarboxylate sodium salt, 1% of magnesium aluminum silicate, 0.25% of xanthan gum, 4.5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of an organosilicon defoamer and the balance of deionized water;
The preparation method comprises the steps of sequentially placing active ingredients, a surfactant and other functional additives in a reaction kettle according to the formula proportion, adding water, uniformly mixing, shearing at a high speed, performing wet sanding, and finally homogenizing and filtering to obtain the suspending agent.
Preparation example 3:
12% of a compound of formula (I) Feneptamidoquin emulsifiable concentrate (2:10)
The preparation formula comprises 2% of a compound of formula (I), feneptamidoquin%, 1.5% of BHT, 10% of propylene carbonate, 15% of DMF, 5% of cyclohexanone, 3.5% of calcium dodecyl benzene sulfonate, 10% of fatty alcohol polyoxyethylene ether and the balance of methyl oleate;
The preparation method comprises the steps of adding active ingredients into a cosolvent according to the formula proportion of the embodiment, adding a surfactant and other functional additives into the cosolvent, and stirring and uniformly mixing in a stirring and mixing kettle to obtain the emulsifiable concentrate.
Preparation example 4:
24% of a compound of formula (I) Fenopyramid suspension (8:16)
The formulation formula comprises 8% of a compound of formula (I), fenopyramid% of a polyether, 4% of a phenethyl phenol polyether phosphate, 3% of dioctyl sodium sulfosuccinate, 1.5% of a sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.2% of xanthan gum, 4.5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of an organosilicon defoamer and the balance of deionized water;
The preparation method is the same as that of preparation example 2.
Preparation example 5:
48% of the compound of formula (I) Fenopyramid Water dispersible granules (16:32)
The formulation formula comprises 16% of a compound of formula (I), fenopyramid% of sodium dodecyl sulfate, 3.5% of polynaphthalene formaldehyde sulfonate (270K), 8% of fatty alcohol polyoxyethylene ether sodium sulfate, 1% of sodium polycarboxylate BX 2%, 6.5% of sodium lignin sulfonate, 7% of attapulgite and the balance of kaolin;
The preparation method comprises the steps of adding active ingredients into a carrier according to the formula proportion, adding a surfactant and other functional additives into the carrier, mixing, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening to obtain a water dispersible granule product, or spraying water, granulating, drying and screening the crushed powder in a boiling granulator to obtain the product.
Field efficacy test of wheat leaf rust
The test basis is that the bactericide is used for preventing and treating cereal rust disease (leaf rust, strip rust and stalk rust) (GB/T17980.23-2000) by referring to pesticide field efficacy test criterion (I);
test site is Shandong Shouguang Nanjun Fan Cunxiao wheat field;
test target wheat leaf rust (Puccinia recondita. F.sp.tritici.);
Test crop and variety wheat (b.sativa 98);
the test setting is that each cell is distributed according to random group, and the periphery of each cell is provided with protection rows. Each cell was randomly arranged with 20m2 per cell per 4 repetitions of the process.
Test agent:
Table 3 test treatments and dosage amounts
| Medicament | Dosage g a.i/hm2 |
| 22% Of a compound of formula (I), feneptamidoquin suspension (8:14) | 75 |
| 24% Of a compound of formula (I) Fenopyramid suspension (8:16) | 75 |
| 30% Of a compound of formula (I) as suspending agent | 100 |
| 15% Feneplamidoquin suspension | 200 |
| 20% Fenopyramid suspension | 250 |
| Clear water control | / |
The test method comprises the steps of applying the pesticide in the early stage of wheat leaf rust (wheat grouting period) by adopting a conventional spraying method, applying the pesticide once for the second time at intervals of 7d, and uniformly spraying the pesticide on the front and back surfaces of leaves for a total of two times, wherein the field water consumption is 600L/hm2.
Investigation and statistical methods 10d after the last dose. Five diagonal samples were randomly selected per cell, each of which was investigated for 20 plants, each of which was investigated for the top 3 leaves (including the flag leaves if any), and rated as a percentage of the total leaf area of the lesions on each leaf.
The grading method comprises the following steps:
grade 0, no disease;
stage 1, the area of the disease spots accounts for less than 5% of the whole leaf area;
Stage 3, wherein the area of the lesion accounts for 6% -25% of the whole leaf area;
stage 5, wherein the area of the lesion accounts for 26% -50% of the whole leaf area;
Stage 7, the area of the lesion accounts for 51% -75% of the whole leaf area;
Grade 9, the area of the disease spots accounts for more than 75% of the whole leaf area.
Drug effect calculation method
The disease index and the control effect are calculated by the following method
During the test period, the wheat in each treatment cell is observed to grow well, and no phytotoxicity is seen in each treatment.
Cell yield was measured during wheat harvest.
Test results:
As shown in Table 4, the compound shown in (I) and Feneptamidoquin, fenopyramid show good control effect on wheat leaf rust. After the last application, the control effect of 10d,22% of the compound shown in the formula (I) and Feneptamidoquin suspension (8:14) and 24% of the compound shown in the formula (I) and Fenopyramid suspension (8:16) on wheat leaf rust is respectively 83.10% and 85.13%, and the control effect is excellent and is remarkably higher than that of a single dose of control.
TABLE 4 results of field efficacy tests for controlling wheat leaf rust by different treatments
Note that the disease index and the control effect are the average of the replicates in the table, and that the differences between groups are statistically significant (p < 0.05) in the lower case letters of the same column.
As shown in Table 5, 22% of the compound of formula (I) & Feneptamidoquin suspension (8:14), 24% of the compound of formula (I)
Fenopyramid the suspending agent (8:16) has obvious yield increasing effect on wheat, and the yield increasing rate is 18.95% and 16.72% respectively.
TABLE 5 influence of different treatments on wheat yield
In the test process, the conditions of pesticide injury, affected crop growth and development and the like are not recorded, and the test agent is safe to crops in the recommended dosage. No effect of the agent on the non-target organisms was found in the dose range. No extreme weather conditions such as heavy rainfall, strong wind, etc. were recorded during the test.
The field efficacy plot test result shows that the compound shown in the formula (I) and Feneptamidoquin, fenopyramid are compounded in the wheat leaf rust prevention and treatment process, so that the compound has a higher prevention and treatment effect, not only reduces the disease index, but also has an obvious prevention and treatment effect, has no great influence on the yield, and the mixed preparation also has an obvious yield increase effect. In addition, according to observation after the medicine, the wheat growth in each treatment area is normal, and no phytotoxicity phenomenon occurs, which indicates that the wheat growth of each medicine is safe under the test dosage.
Through indoor toxicity measurement and field experiments, the compound shown in the formula (I) and the composition prepared by Feneptamidoquin, fenopyramid have very good control effect on wheat leaf rust and have wide application prospect. The sterilization composition or the preparation thereof obtained by compounding has remarkable prevention effect, has the characteristics of high efficiency, broad spectrum, low residue, long lasting period, strong systemic property and the like, and has no drug damage to crops caused by the compounded medicament in experiments, so that the production cost and the use cost can be reduced under the condition that the sterilization synergistic effect of the obtained sterilization composition or preparation is improved, and the sterilization composition or preparation is safe to crops.
While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto, and it is therefore intended that the invention as defined in the appended claims be interpreted as broadly as possible without departing from the spirit of the invention.