Optical instrument based on multifunctional fluorescence chemiluminescence imagingTechnical Field
The invention relates to the technical field of optical instruments, in particular to an optical instrument based on multifunctional fluorescent chemiluminescence imaging.
Background
The fluorescent chemiluminescence imaging system is a precise instrument for detecting fluorescence and chemiluminescence signals of biological and chemical samples, and excites the samples through excitation light with specific wavelength during fluorescence imaging, chemiluminescence imaging automatically emits light by chemical reaction, then an optical system collects signals, a high-sensitivity detector (such as CCD and CMOS) converts the optical signals into electric signals, and then the electric signals are processed by software to display images.
The Chinese patent application No. CN218067653U discloses an optical instrument based on multifunctional fluorescent chemiluminescence imaging, which particularly belongs to the field of optical instruments, and comprises an instrument main body, wherein a placing groove is formed in one side of the instrument main body, one side of the groove is communicated with a power line, one end of the power line is fixedly connected with a lithium battery, the other side of the instrument main body is fixedly connected with an LED lamp strip, one side of the placing groove is rotationally connected with a groove cover, two moving ports are formed in the groove cover, a button is connected in the middle of the moving ports in a sliding manner, one end of the button is fixedly connected with a sliding block, the bottom of the sliding block is connected with a first spring, one side of the spring is fixedly connected with a moving box (one side of the moving box is fixed with the groove cover), and the technical scheme increases the electric quantity storage structure and the moving convenience of the instrument, improves the use effect and has practicability.
In the fluorescent chemiluminescence imaging detection process, the box door needs to tightly seal the sample chamber to construct a dark or low-light environment, which is a key for ensuring detection accuracy and sensitivity, because once external stray light enters, background noise can be obviously increased to interfere weak fluorescent or chemiluminescence signal detection to cause result deviation, the tightness of the sample chamber is critical, the sealing of the sample chamber is mainly dependent on the tight sealing of a sealing gasket fixedly connected with the box door, however, the sealing gasket is easy to age and deform, the specific performance of the sealing gasket is not easy to observe after the box door is closed, the sealing condition in the sample chamber is also difficult to judge, and a user is difficult to intuitively judge the sealing condition of the sample chamber.
Therefore, the invention provides an optical instrument based on multifunctional fluorescence chemiluminescence imaging, and aims to solve the problems.
Disclosure of Invention
The present invention aims to provide an optical instrument based on multifunctional fluorescence chemiluminescence imaging, so as to solve the problems set forth in the background art:
In order to achieve the above object, the present invention provides the following technical solutions:
The optical instrument based on the multifunctional fluorescence chemiluminescence imaging comprises a fluorescence chemiluminescence imaging instrument, wherein a box door is movably arranged at the lower part of the fluorescence chemiluminescence imaging instrument, a packaging plate is movably arranged in the box door, a sealing gasket is fixedly connected to the side surface of the packaging plate, a sample chamber is arranged at the lower part of the fluorescence chemiluminescence imaging instrument, a sealing frame is fixedly connected to the front part of the sample chamber, and the sealing gasket is movably arranged with the sealing frame;
The sample chamber is internally and slidably connected with a sample table, the bottom surface of the sample table is fixedly connected with a carriage, the bottom surface of the packaging plate is movably connected with a connecting plate, and the connecting plate is in sliding connection with the carriage;
One side of the packaging plate is fixedly connected with a photoelectric detector, and the other side of the packaging plate is fixedly connected with a buzzer.
By adopting the technical scheme, the packaging plate is sealed with the sealing frame under the pushing of the telescopic spring, so that the inside of the sample chamber is kept sealed, and an external light source is not easy to enter;
When the box door is opened, the sample platform synchronously moves out, and when the box door is closed, the sample platform automatically resets, so that the sample platform is convenient to automatically pop up and stretch out and draw back;
When the box door is closed, the photoelectric detector detects the sample chamber, when an external power supply enters the sample chamber, the photoelectric detector transmits signals to the microcontroller, and the microcontroller controls the buzzer to sound, so that the sample chamber is not tightly sealed, and early warning can be timely performed when a light source enters.
Preferably, the box door is movably installed with the fluorescence chemiluminescence imager through a hinge, and a sealing gasket at the side surface of the packaging plate is in sealing contact with the sealing frame.
By adopting the technical scheme, the sealing property is enhanced through the sealing gasket, so that the packaging plate is in sealing connection with the sample chamber.
Preferably, the packaging plate is fixedly connected with a plurality of connecting frames, the inner side of the box door is fixedly connected with a plurality of telescopic brackets, and the positions of the connecting frames correspond to the positions of the telescopic brackets.
By adopting the technical scheme, the telescopic bracket is fixedly connected with the box door, and the connecting frame is movably arranged at the side surface of the telescopic bracket, so that the connecting frame drives the packaging plate to synchronously move.
Preferably, a through hole is formed in the connecting frame, a screw is movably installed in the through hole, one end of the screw is fixedly connected with a limiting plate, and the limiting plate is located in the telescopic bracket.
By adopting the technical scheme, the screw is prevented from being separated from the telescopic bracket by the limiting plate.
Preferably, the connecting frame is provided with a screw hole, the other end of the screw rod is fixedly connected with the screw hole of the connecting frame, the screw rod is sleeved with a telescopic spring, and the telescopic spring is positioned between the telescopic bracket and the connecting frame.
Through adopting above-mentioned technical scheme, through the screw structure on the link for screw rod, limiting plate convenient to detach.
Preferably, the sliding sleeve is fixedly connected to the two sides of the sample chamber, a plurality of rollers are rotationally connected to the sliding sleeve, the two sides of the sample table are slidably connected to the sliding sleeve, and the two sides of the sample table are rotationally connected with the rollers.
Through adopting above-mentioned technical scheme, through the gyro wheel structure for the outward of stable, inwards removal of sample platform be convenient for.
Preferably, a sliding groove is formed in the sliding frame, one end of the connecting plate is fixedly connected with a first connecting seat, and the first connecting seat is slidably connected in the sliding groove.
Through adopting above-mentioned technical scheme, the sliding movement of connecting seat No. one in the spout for the one end of connecting plate is convenient for activity and removes.
Preferably, the lower part of the packaging plate is fixedly connected with a second connecting seat, and the other end of the connecting plate is provided with a through hole and is rotationally connected with the second connecting seat through the through hole.
By adopting the technical scheme, the other end of the connecting plate synchronously moves along with the box door.
Preferably, the bottom surface position fixedly connected with fixed plate of sample room, fixedly connected with locating lever on the fixed plate, sliding connection has the slide on the locating lever, the cover is equipped with reset spring on the locating lever, just reset spring is located the position between fixed plate and the slide, locating lever, reset spring, fixed plate and slide are located the below position of sample platform.
By adopting the technical scheme, the return spring is compressed when the sample platform stretches out, and the sample platform is pushed to shrink by the return spring when the box door is closed.
Preferably, the packaging plate is provided with a mounting bin, the buzzer is fixedly connected in the mounting bin, a microcontroller is fixedly connected to the upper position of the buzzer in the mounting bin, pins of the photoelectric detector penetrate through the packaging plate and are fixedly connected with the microcontroller, the photoelectric detector array is arranged at the inner side of the packaging plate, and the photoelectric detector stretches into the sample chamber.
By adopting the technical scheme, the photoelectric detector monitors the light source in the sample chamber, and when the external light source enters, the buzzer sounds to warn the user.
Compared with the prior art, the invention has the beneficial effects that:
1) The sealing frame is sealed through the sealing gasket of packing plate and side, and the expansion spring promotes the link for the packing plate is good to the sealed effect of sample room, and external light source is difficult for getting into.
2) When the box door of the optical instrument is opened, the sample platform slides out synchronously, so that the sample platform is convenient to extend automatically, and when the box door is closed, the sample platform contracts synchronously, so that the convenience in placing samples is improved.
3) When the box door is opened, the buzzer of the optical instrument is driven by an external light source, the photoelectric detector detects signals, so that the buzzer is sounded, and whether the buzzer operates normally or not is calibrated.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic view of a sample chamber according to the present invention;
FIG. 3 is a schematic view of the package board of the present invention in a box door;
FIG. 4 is a schematic view of the structure of the mounting bin of the invention;
FIG. 5 is an enlarged view of FIG. 4 at A;
FIG. 6 is a schematic view of a sample stage according to the present invention;
FIG. 7 is a schematic view of the inverted structure of the sample stage of the present invention;
FIG. 8 is a schematic structural view of a sliding sleeve according to the present invention;
fig. 9 is a schematic structural view of the carriage and the connection plate according to the present invention.
The reference numerals in the figure indicate that 1, a fluorescence chemiluminescence imager, 2, a box door, 3, a hinge, 4, a sample chamber, 5, a sample table, 6, a packaging plate, 7, a sealing gasket, 8, a sealing frame, 9, a telescopic bracket, 10, a connecting frame, 11, a telescopic spring, 12, a screw rod, 13, a limiting plate, 14, a mounting bin, 15, a photoelectric detector, 16, a microcontroller, 17, a buzzer, 18, a sliding sleeve, 19, a roller, 20, a sliding carriage, 21, a sliding chute, 22, a connecting plate, 23, a first connecting seat, 24, a second connecting seat, 25, a positioning rod, 26, a reset spring, 27, a fixing plate, 28 and a sliding seat.
Detailed Description
Referring to fig. 1,3, 4 and 5, an optical apparatus based on multifunctional fluorescence chemiluminescence imaging includes a fluorescence chemiluminescence imaging apparatus 1, a box door 2 movably mounted at a lower position of the fluorescence chemiluminescence imaging apparatus 1, a package plate 6 movably mounted in the box door 2, the package plate 6 positioned right in front of a sample chamber 4, a sealing pad 7 fixedly connected at a side position of the package plate 6, the sealing pad 7 positioned between the package plate 6 and a sealing frame 8 for enhancing sealing performance, the sample chamber 4 provided at the lower position of the fluorescence chemiluminescence imaging apparatus 1, the sealing frame 8 fixedly connected at a front position of the sample chamber 4, and the sealing pad 7 movably mounted with the sealing frame 8, the package plate 6 moving forward to make the sealing pad 7 and the sealing frame 8 contact each other.
The box door 2 is movably installed with the fluorescence chemiluminescence imager 1 through the hinge 3, so that the box door 2 is convenient to rotate outwards, and the sealing gasket 7 on the side face of the packaging plate 6 is in sealing contact with the sealing frame 8.
Fixedly connected with a plurality of link 10 on the packing plate 6, link 10 are located sealed frame 8 outside position, and chamber door 2 inboard position fixedly connected with a plurality of telescopic bracket 9, and the position of link 10 corresponds each other with telescopic bracket 9's position for telescopic bracket 9, link 10 are located same horizontal line.
The connecting frame 10 is internally provided with a through hole, the through hole is internally movably provided with a screw rod 12, one end of the screw rod 12 is fixedly connected with a limiting plate 13, the limiting plate 13 moves backwards on the telescopic bracket 9, then screw holes on the screw rod 12 and the connecting frame 10 are separated from each other through rotation, the limiting plate 13 and the screw rod 12 are convenient to move out of the telescopic bracket 9, and the limiting plate 13 is positioned in the telescopic bracket 9.
Screw holes are formed in the connecting frame 10, the other end of the screw rod 12 is fixedly connected with the screw holes of the connecting frame 10, the telescopic spring 11 is sleeved on the screw rod 12, and the telescopic spring 11 is compressed, so that the telescopic spring 11 can push the connecting frame 10 to move outwards, the packaging plate 6 is pushed to move outwards, and the telescopic spring 11 is located between the telescopic bracket 9 and the connecting frame 10.
When the optical instrument is used, the packaging plate 6 is positioned at the outer side of the sealing frame 8, the sealing gasket 7 is positioned between the packaging plate 6 and the sealing frame 8, and a plurality of connecting frames 10 fixedly connected to the packaging plate 6 are pushed outwards by the telescopic springs 11, so that the packaging plate 6 and the sealing gasket 7 on the side face squeeze the sealing frame 8, the packaging plate 6 is wrapped at the inner position by the box door 2, the edge position of the box door 2 is mutually attached to the outer side position of the sealing frame 8 outside the sample chamber 4, and therefore, an outer sealing structure is arranged at the outer side position of the packaging plate 6 again, so that the sealing effect of the sample chamber 4 is better;
when the packaging plate 6 needs to be disassembled, the limiting plate 13 is rotated, the limiting plate 13 drives the screw rod 12 to rotate, one end of the screw rod 12 is screwed out of a screw hole in the connecting frame 10, then the limiting plate 13 and the screw rod 12 are moved backwards in the telescopic bracket 9, the screw rod 12 and the limiting plate 13 are moved out of the telescopic bracket 9, the connecting frame 10 is separated from the telescopic bracket 9, the telescopic springs 11 between the connecting frame 10 and the telescopic brackets are synchronously separated, and the packaging plate 6 and the telescopic springs 11 are convenient to replace and maintain.
Referring to fig. 1 to 9, the difference of the combination of the embodiment 1 is that the sample chamber 4 is slidably connected with the sample stage 5, the sample stage 5 stretches in the sample chamber 4, the bottom surface of the sample stage 5 is fixedly connected with the carriage 20, the sample stage 5 drives the carriage 20 to synchronously move, the bottom surface of the package plate 6 is movably connected with the connecting plate 22, and the connecting plate 22 is slidably connected with the carriage 20, so that the sample stage 5 can be pulled out through the connecting plate 22 and the carriage 20 when the package plate 6 is synchronously opened with the box door 2.
The slide sleeve 18 is fixedly connected to the two side positions of the sample chamber 4, the slide sleeve 18 is located the two side positions of the sample table 5, a plurality of rollers 19 are rotationally connected to the slide sleeve 18, the rollers 19 are equidistantly distributed in the slide sleeve 18, the two side positions of the sample table 5 are slidingly connected in the slide sleeve 18, the two side positions of the sample table 5 are rotationally connected with the rollers 19, and when the sample table 5 moves out, the rollers 19 synchronously rotate, so that the movement of the sample table 5 is more stable.
The sliding chute 21 is formed in the sliding frame 20, a first connecting seat 23 is fixedly connected to one end of the connecting plate 22, the first connecting seat 23 slides in the sliding chute 21 and can be prevented from being separated from the sliding chute 21, and the first connecting seat 23 is slidably connected in the sliding chute 21.
The lower part of the packaging plate 6 is fixedly connected with a second connecting seat 24, the other end of the connecting plate 22 is provided with a through hole, the second connecting seat 24 is rotationally connected with the second connecting seat 24 through the through hole, and the second connecting seat 24 upwards passes through the corresponding through hole on the connecting plate 22 and is fixedly connected with the packaging plate 6.
The bottom surface position fixedly connected with fixed plate 27 of sample room 4, fixedly connected with locating lever 25 on the fixed plate 27, the both ends of locating lever 25 respectively with fixed plate 27, the mutual fixed connection of inner wall of sample room 4, sliding connection has slide 28 on the locating lever 25, the cover is equipped with reset spring 26 on the locating lever 25, and reset spring 26 is located the fixed plate 27 and slide 28 between the position for when slide 28 removes, reset spring 26 can be compressed, locating lever 25, reset spring 26, fixed plate 27 and slide 28 are located the below position of sample platform 5.
When the optical instrument is used, after a sample is manufactured, the box door 2 is opened, the box door 2 rotates to the side through the hinge 3, the box door 2 moves outwards, the telescopic bracket 9 moves synchronously, after the limiting plate 13 and the telescopic bracket 9 are contacted with each other, the telescopic bracket 9 drives the limiting plate 13, the limiting plate 13 drives the packaging plate 6 to move synchronously through the screw rod 12 and the connecting frame 10, the packaging plate 6 drives one end of the connecting plate 22 to move outwards through the second connecting seat 24, the first connecting seat 23 at the other end of the connecting plate 22 slides in the sliding groove 21 and pulls the sliding frame 20 outwards, the sliding frame 20 drives the sample platform 5 to move outwards, the sample platform 5 moves outwards in the sliding sleeve 18, the rollers 19 corresponding to the two sides of the sample platform 5 synchronously rotate, the sliding seat 28 moves synchronously on the positioning rod 25, the reset spring 26 on the positioning rod 25 is compressed, the sample platform 5 stretches outwards automatically, and the sample is placed on the sample platform 5 after the sample platform 5 moves to a proper position; then the box door 2 is reversely pushed, the box door 2 and the packaging plate 6 on the side face of the box door 2 are gradually close to the sealing frame 8, at the moment, the elastic force of the reset spring 26 is larger than the pressure exerted by the sliding seat 28, the second connecting seat 24 at one end of the connecting plate 22 synchronously moves along with the packaging plate 6, the first connecting seat 23 at the other end of the connecting plate 22 reversely slides in the sliding groove 21, the sliding seat 28 reversely moves on the positioning rod 25, the sample table 5 is reversely pushed in the sliding sleeve 18, the packaging plate 6, the sealing gasket 7 and the sealing frame 8 are mutually closed so as to be blocked, and therefore, the automatic shrinkage of the sample table 5 is realized, the sample table 5 does not need to be manually pulled when a sample is placed, and the convenience of operation is improved.
Referring to fig. 1 to 9, the difference between the combination of the embodiment 2 and the embodiment is that a photo detector 15 is fixedly connected to one side of the package board 6, the photo detector 15 detects a light source in the sample chamber 4, a buzzer 17 is fixedly connected to the other side of the package board 6, the buzzer 17 plays a role in alarming, a mounting bin 14 is provided on the package board 6, the buzzer 17 is fixedly connected to the mounting bin 14, the microcontroller 16 and the buzzer 17 are protected by the outside box door 2, the microcontroller 16 is fixedly connected to the upper position of the buzzer 17 in the mounting bin 14, pins of the photo detector 15 penetrate through the package board 6 and are fixedly connected with the microcontroller 16, the photo detector 15 is arrayed at the inner side of the package board 6, and the photo detector 15 extends into the sample chamber 4.
The invention comprises the following steps: when the optical instrument is used, the buzzer 17 and the photoelectric detector 15 are started, when a sample is detected, the box door 2 is firstly opened, the packaging plate 6 is separated from the sample chamber 4 after the box door 2 is opened, an external light source enters the sample chamber 4, the photoelectric detector 15 moves along with the movement of the box door 2, so that the photoelectric detector 15 is exposed in the external light source, the photoelectric detector 15 transmits a signal to the microcontroller 16, after the microcontroller 16 judges the external light source, the buzzer 17 connected with a telecommunication is started, the buzzer 17 is controlled to sound in a low sound mode, so that the photoelectric detector 15 and the buzzer 17 are tested in the external light source to judge whether the photoelectric detector 15 and the buzzer 17 operate normally or not, if the buzzer 17 does not sound, the photoelectric detector is damaged, the photoelectric detector needs to be maintained and replaced in time, the box door 2 is closed after the sample is placed on the sample table 5, after the packaging plate 6 and the sealing frame 8 are mutually sealed, the sample chamber 4 is in a black box state under the condition that the sample chamber does not start to work, no external light source is arranged, the buzzer 17 stops ringing after the photoelectric detector 15 does not detect the external light source, so that the sealing state of the sample chamber 4 is good, if the buzzer 17 does not stop ringing, the sealing effect of the box door 2 is poor, the external light source still enters, when the light source is excited to emit light in the fluorescent chemical imaging process, the photoelectric detector 15 detects a signal, and synchronously starts the buzzer 17 to make a low-noise sound, so that workers are reminded that the inside of the sample chamber 4 is working, the sealing state in the sample chamber 4 is convenient to detect, and the external light source is prevented from entering the inside to cause interference and influence the accuracy of results.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present invention, and are not intended to limit the invention, and that various changes and modifications may be made therein without departing from the spirit and scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.