Program cooling instrumentTechnical Field
The invention relates to cooling equipment, in particular to a program cooling instrument.
Background
The program cooling instrument is one kind of equipment for controlling temperature rate to freeze biological sample with liquid nitrogen, and is used widely in genetic engineering, bone marrow transplantation, umbilical cord blood transplantation, tumor research, hematology research, medicine and vaccine production, tissue cell engineering, virus and bacteria preservation and other medicine research fields.
The existing program cooling instrument generally has the following defects that 1, the program cooling instrument mainly uses liquid nitrogen for evaporation as a cold source to cool biological samples, when the cooling speed is too high, the evaporation capacity of the liquid nitrogen cannot be accurately controlled, so that the temperature deviates from expectations and has larger fluctuation, 2, the container is unfavorable for convection, the existing freezing container is generally a freezing tube and a freezing bag, the volume is small, internal liquid is difficult to convect, the phase change is not synchronous due to uneven cooling, the freezing survival rate is reduced, 3, cell suspension is unstable, cell precipitation and a protective agent are separated before the phase change to influence the freezing effect, 4, the temperature measurement is inaccurate, the temperature of the samples is measured by using a probe to measure the reference samples, the measured value is non-representative due to the difference between the samples and the reference samples, and the cooling characteristic is inconsistent.
Disclosure of Invention
The invention aims to solve the technical problem of providing a program cooling instrument which adopts a refrigerant for cooling and has an oscillating table.
In order to solve the technical problems, the technical solution of the program cooling instrument of the invention is as follows:
The cooling box comprises a freezing storage slot, a controller and a plurality of precooling pools with different precooling temperature values, wherein the precooling pools are provided with liquid inlet branch pipes and liquid outlet branch pipes, the liquid inlet branch pipes are connected with liquid outlets of the cooling box through liquid inlet main pipes, the liquid outlet branch pipes are connected with liquid inlets of the cooling box through liquid inlet main pipes, the precooling pools form a loop through the liquid inlet branch pipes, the liquid outlet main pipes and the liquid inlet main pipes, liquid refrigerants are filled in the loop, the liquid inlet branch pipes are provided with first valves, the liquid outlet branch pipes are provided with pump bodies for pushing the liquid refrigerants to circulate, the precooling pool comprises a recovery bin and a heat preservation bin located below the recovery bin, the recovery bin is connected with the heat preservation bin through a pipeline, the liquid inlet branch pipes are connected with the recovery bin, the heat preservation bin is provided with a refrigerating device at the side edge, the cooling box is provided with an oscillating table below the cooling box, and the controller is respectively connected with the cooling box, the oscillating table and the first valves and the heat preservation bin.
The side of the refrigerating device is connected with a radiator, and the radiator is connected with the controller through a wire.
The freezing storage slot is internally provided with a freezing storage container temperature probe, the outer side of the freezing storage slot in the cooling box is also provided with a liquid refrigerant temperature probe, and the freezing storage container temperature probe and the liquid refrigerant temperature probe are respectively connected with the controller through wires.
And a heat insulation pad is connected between the wall of the freezing storage slot and the temperature probe of the freezing storage container.
The precooling pool is provided with three.
The refrigerating device is a refrigerating sheet or an air conditioner compressor.
The temperature probe is inserted into the thermal insulation bin and connected with the controller through a wire.
The invention has the following technical effects:
1. According to the invention, the oscillating table is arranged below the cooling box, the sample is uniformly mixed by the oscillating table module before the phase change of the cell suspension, the cell precipitation can be prevented in the cooling process, the temperature of the homogenized sample is simultaneously changed, and the freezing survival rate is improved during shrinkage;
2. setting a freezing container temperature probe and a liquid refrigerant temperature probe, setting a plurality of probes to monitor the temperature among samples, and regulating the liquid refrigerant quantity and the cooling speed according to the temperature, wherein the precision is superior to that of a single probe reference sample measuring method;
3. The liquid refrigerant is used as a medium, a pre-cooling pool is arranged, the liquid refrigerant is led into a sample cavity according to the requirement to cool, when the phase change releases latent heat, the specific heat of the liquid refrigerant is large, the pre-cooling is controllable, the temperature is precisely controlled, the temperature is fast, the heat is carried, and the freezing effect is excellent;
4. By designing a plurality of precooling ponds with different precooling temperature values, the liquid refrigerant is sent into the precooling pond with one level lower than the precooling temperature value of the liquid refrigerant by the precooling pond step with the highest precooling temperature value for recycling, and the liquid refrigerant is more energy-saving.
Drawings
The invention is described in further detail below with reference to the attached drawings and detailed description:
FIG. 1 is a schematic diagram of a program cooling apparatus according to the present invention;
FIG. 2 is a schematic diagram of the structure of a pre-cooling tank;
FIG. 3 is a top view of the cooling box;
fig. 4 is a cross-sectional view of the cooling box.
Detailed Description
The invention is further described in detail below with reference to the accompanying drawings.
See fig. 1-4.
A program cooling instrument comprises a cooling box 1 with a freezing slot 11, a controller 2 and a plurality of precooling tanks 3 with different precooling temperature values, wherein the precooling tanks 3 are provided with three liquid inlet branch pipes 31 and liquid outlet branch pipes 32, the liquid inlet branch pipes 31 are connected with liquid outlets of the cooling box 1 through liquid outlet main pipes 33, the liquid outlet branch pipes 32 are connected with liquid inlets of the cooling box 1 through liquid inlet main pipes 34, the precooling tanks 3 form a loop with the cooling box 1 through the liquid inlet branch pipes 31, the liquid outlet branch pipes 32, the liquid outlet main pipes 33 and the liquid inlet main pipes 34, liquid refrigerants are filled in the loop, a first valve 35 is arranged on each liquid inlet branch pipe 31, a pump body 5 for pushing the liquid refrigerants to circulate is arranged on each liquid outlet branch pipe 32, each precooling tank 3 comprises a recovery bin 41 and a heat preservation bin 42 positioned below the recovery bin 41, the recovery bin 41 is connected with the heat preservation bin 42 through a pipeline, the liquid inlet branch pipes 32 are connected with the recovery bin 41 through liquid inlet branch pipes 34, the liquid inlet branch pipes 32 are connected with the heat preservation bin 42, a vibration table 4 is arranged on the side of the vibration table, a cooling device is arranged on the vibration table, a cooling device 4 is arranged on the vibration table, and a cooling device 4 is arranged on the vibration table 4, and a cooling device is arranged on the cooling device 4, and a cooling device is arranged on the cooling table 4.
Preferably, a radiator 7 is connected to the side of the refrigerating device, and the radiator 7 is connected to the controller 2 through a wire.
When the liquid-phase precooling device is used, a sample (the sample is a cell suspension arranged in a freezing tube or a freezing bag and is formed by suspending cells in a freezing protection liquid and is a target of program cooling) is inserted into a freezing slot 11, the sample can be uniformly mixed by the oscillating table 4 in the sample cooling process, in the sample phase-change process (the phase change is a process of converting the sample from a liquid state to a solid state in the program cooling process, the process releases heat and damages the cells, the phase change time is short, the temperature difference is small), cell precipitation can be prevented by the oscillating table 4, the temperature of the homogenized sample can realize simultaneous phase change, and the freezing survival rate is improved during shrinkage.
The invention specifically discloses a cooling application description of a sample, for example, the invention is provided with three precooling ponds 3 with different precooling temperature values, specifically, the three precooling ponds 3 are a precooling pond with high precooling temperature value, a precooling pond with medium precooling temperature value and a precooling pond with low precooling temperature value from high precooling temperature value to low precooling temperature value, firstly, a liquid refrigerant is positioned in the precooling pond with high precooling temperature value, after being sent into a cooling box 1 through a pump body 5 for heat exchange, the liquid refrigerant is sent into a recovery bin 41 of the precooling pond with medium precooling temperature value, then the liquid refrigerant enters a heat preservation bin 42 of the precooling pond with medium precooling temperature value by gravity, the liquid refrigerant is cooled to a set temperature value through a refrigerating device of the precooling pond with medium precooling temperature value, then the liquid refrigerant is sent into the precooling box 1 after being sent into the precooling box 1 through the pump body 5 with medium precooling temperature value, after the liquid refrigerant is sent into the precooling pond with low precooling temperature value is finally, the liquid refrigerant is sent into the precooling box with low precooling temperature value after the liquid refrigerant is sent into the precooling box with low precooling temperature value is cooled by the heat preservation bin 42; the liquid refrigerant is subjected to gradient cooling through the three precooling tanks 3 with different precooling temperature values, so that the energy is saved.
The invention is characterized in that a freezing container temperature probe 21 is arranged in a freezing slot 11, a liquid refrigerant temperature probe 22 is also arranged outside the freezing slot 11 in a cooling box 1, the freezing container temperature probe 21 and the liquid refrigerant temperature probe 22 are respectively connected with a controller 2 through leads, a heat insulation pad 23 is connected between the wall of the freezing slot 11 and the freezing container temperature probe 21, the heat insulation pad 23 is used for preventing the temperature of the wall of the slot from interfering with the temperature measurement of the freezing tube or the freezing bag (namely the freezing container) when the temperature of the wall of the slot is different from the surface temperature of the freezing tube or the freezing bag (namely the freezing container), the invention measures the surface temperature of a sample through the freezing container temperature probe 21 and calculates the actual temperature of the sample according to the parameters of the container, a cooling model with different sample container and volume is pre-built, the freezing container temperature probe 21 and the liquid refrigerant temperature probe 22 are arranged to monitor the temperature of the sample and the liquid refrigerant according to the liquid refrigerant volume and the cooling speed, the precision is superior to that of the single probe measuring method, the freezing container temperature probe 21 can be arranged in the freezing slot 11 according to the requirement, and all the freezing container temperature probes 11 are not required to be arranged, and the freezing container temperature probe 21 can be conveniently connected with a temperature detector through the leads 51 and the temperature probe 51 by the temperature sensor 42, and the temperature probe 51 is conveniently connected with the temperature probe 51 through the leads.