Microbial fluorescence detection system and detection methodTechnical Field
The invention relates to the technical field of microorganism gene detection, in particular to a microorganism fluorescence detection system and a microorganism fluorescence detection method.
Background
Food safety is considered a global public health problem, and food-borne pathogenic bacteria are an important aspect of food safety. Vegetables and fruits are foods with rich nutrition, and the vegetables have high water content, so that pathogenic microorganisms are very easy to be infected in the processing and transportation processes, thereby causing pathogenic diseases and endangering human health. Coli, salmonella, listeria monocytogenes, staphylococcus aureus, and norovirus, are common vegetable-borne pathogenic microorganisms.
Vegetables are food necessary for daily life, and pollution of pathogenic microorganisms on the vegetables is closely related to the health of consumers, so that detection of the pathogenic microorganisms on the vegetables, particularly on raw vegetables, is important for guaranteeing the health of people.
Fluorescence imaging is an indispensable tool in biological research, especially in cellular research. By staining cells or organelles with various fluorophores and imaging them under a microscope in an enlarged manner, a large number of color-coding processes can be quantitatively characterized and studies of cellular gene expression based on chromosome dynamics can be carried out. Fluorophores have greatly improved cell research by introducing high quantum yield fluorescent dyes and multiplex staining methods into this field. This enables researchers to study several organelles and their interactions simultaneously in the same FOV (field of view) while having high contrast. The microbial fluorescent detection system is a technology for detecting and quantifying microorganisms, and is mainly used for monitoring and detecting microbial contamination in foods, such as bacteria, fungi and the like.
The application discloses a fluorescence spectrum detection system of microorganisms, which has the technical scheme that the fluorescence spectrum detection system comprises a laser light source for emitting laser to a microorganism sample to be detected, which is borne on a light-transmitting platform, for exciting fluorescent dye to emit fluorescence, the light-transmitting platform is arranged on a focal plane of a micro lens group, the micro lens group amplifies fluorescent light transmitted by the light-transmitting platform to form parallel light, a filter for filtering the parallel light, a first condensing lens group condenses the parallel light to form a fluorescent image, the fluorescent image is divided into a plurality of image units by an integral view field unit, the image units are dispersed by a spectrometer, and spectral distribution information obtained by detection of a detector is obtained. According to the application, the fluorescent light rays in the view field of the micro-lens group do not need to be scanned from position to position, so that synchronous dispersion of the fluorescent light rays in the view field of the micro-lens group is realized, the observation efficiency of fluorescent detection of a microorganism sample is improved, the capture of instantaneous change conditions of cells or organelles in microorganisms is facilitated, and the accuracy of microorganism research is improved.
For the relevant matters in the above, the following technical defects are found:
when the microbial fluorescence detection system in the prior art is used, personnel need to manually use a pipette to drop to-be-detected liquid into a plurality of detection cavities on one detection vessel, then put the detection vessel containing to-be-detected liquid into an instrument, finally start the instrument to perform microbial fluorescence detection operation, but the manual liquid dropping mode can cause the following problems to occur:
1. The personnel need to manually control the liquid to be detected to be dripped into the detection vessel by the liquid-transferring gun, and the time and energy of the personnel need to be consumed by manually controlling the liquid to be detected to be dripped by the liquid-transferring gun, so that the complexity of operation is increased, and the detection efficiency and accuracy can be influenced;
2. In the process of dropwise adding detection liquid into the detection dish, the detection liquid is easy to sputter, so that not only can the sample loss be caused, but also the reliability of an experimental result can be influenced, and meanwhile, the experimental environment pollution is also easy to cause, and the workload of cleaning and maintenance is increased.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a microorganism fluorescence detection system.
In order to achieve the purpose, the invention adopts the following technical scheme that the microbial fluorescence detection system comprises a machine body, a detection hole, a driving plate, a mounting plate, a containing bottle, a guide pipe and an automatic liquid dropping mechanism, wherein the detection hole is formed in one side of the machine body, the driving plate is arranged in the detection hole, the mounting plate is arranged on the machine body, one side of the mounting plate is provided with a plurality of containing bottles, one end of the guide pipe is communicated with the containing bottle, the automatic liquid dropping mechanism is positioned on one side of the machine body, the automatic liquid dropping mechanism comprises a supporting plate, a detection dish and an automatic liquid transferring gun, a plurality of detection cavities are formed in the upper surface of the detection dish, the lower surface of the detection dish and the upper surface of the driving plate are positioned in the same plane, one side of the supporting plate is fixedly connected with the machine body, one side of the supporting plate is fixedly connected with a servo motor, the upper surface of the supporting plate is provided with a sliding hole, the inner wall of the sliding hole is slidingly connected with a sliding block, one side of the sliding block is provided with a side threaded through the servo motor, the output end of the sliding block is fixedly connected with one side of the driving plate is fixedly connected with one end of a transmission wheel, one side of the transmission plate is fixedly connected with one end of the transmission plate, one side of the transmission plate is fixedly connected with one circular hole, and the transmission plate is fixedly connected with one end of the transmission plate is fixedly connected with one side, the automatic pipette is located inside the cylinder.
Preferably, the outer wall fixedly connected with locating plate of drum, the locating lever is worn to be equipped with in the side slip of locating plate, the equal fixedly connected with fixed plate in both ends of locating lever, two the fixed plate respectively with one side fixed connection that two fixing bases kept away from each other.
Preferably, the arc surface of the automatic pipetting gun is fixedly connected with a plurality of rectangular blocks, a plurality of rectangular holes are formed in the upper end of the cylinder, and the rectangular blocks are attached to the inner walls of the rectangular holes.
Preferably, one side of the detection vessel far away from the driving plate is fixedly connected with a guide block, and the guide block is in a wedge-shaped structure.
Preferably, one side that the backup pad was kept away from to the organism is equipped with automatic conveying mechanism, automatic conveying mechanism includes welded plate and loading board, one side and the organism fixed connection of welded plate, one side that the organism was kept away from to the welded plate rotates and is connected with initiative reel and driven reel, one side fixedly connected with limiting plate of organism is kept away from to the welded plate, one side fixedly connected with motor of limiting plate, the output and the initiative reel fixed connection of motor, the arc surface transmission of initiative reel and driven reel is connected with a belt, the side rotation of welded plate wears to be equipped with the threaded rod, the one end and the driven reel fixed connection of threaded rod, the arc surface threaded connection of threaded rod has the otic placode, the upper surface fixedly connected with carriage of otic placode, the cross-section "C" shape of carriage, one side and the driving plate laminating mutually of loading board, the upper surface and the driving plate the upper surface of loading board are located the coplanar, the lower surface fixedly connected with board of loading board, one side and the backup pad are close to one side fixed connection of driving board.
Preferably, a guide rod is arranged on one side of the conveying frame in a sliding and penetrating mode, and one end of a short arm end of the guide rod is fixedly connected with the welding plate.
Preferably, the upper surface of loading board has seted up first spacing hole, the second spacing hole has been seted up to the upper surface of drive plate, first spacing hole is linked together with the second spacing hole, the inner wall sliding connection in first spacing hole has the stopper, stopper and the lower fixed surface who detects the ware are connected.
Preferably, one side that the organism is close to the detection hole is equipped with sealing mechanism, sealing mechanism is including rotating the seat, one side and the organism fixed connection of rotating the seat, the inner wall of rotating the seat rotates and wears to have the pivot, the arc surface fixedly connected with linking plate of pivot, one side fixedly connected with closing plate of linking plate, the auxiliary hole has been seted up to the side of closing plate, the arc hole has been seted up to one side of rotating the seat, the centre of a circle and the pivot pivoted axle center in arc hole are coaxial, the inner wall sliding connection in arc hole has the dead lever, one end and one side fixed connection of linking plate of dead lever, the arc surface threaded connection of dead lever has the adjusting ring.
Preferably, a rubber pad is fixedly connected to one side of the adjusting ring, which is close to the rotating seat, and the diameter of the rubber pad is larger than that of the adjusting ring.
Preferably, the arc surface of the rotating shaft is sleeved with a coil spring, and two ends of the coil spring are fixedly connected with the connecting plate and the rotating seat respectively.
The invention also discloses a microorganism fluorescence detection method for continuously detecting by using the microorganism fluorescence detection system, which comprises the following steps:
1) Transferring 50 mu L of pre-detection sample into each detection cavity by using the automatic pipetting gun, and then placing a detection dish into the microbial fluorescence detection;
2) And selecting detection conditions, and acquiring detection results in real time, wherein the acquisition time of the detection results does not exceed 27 minutes.
Compared with the prior art, the invention has the advantages and positive effects that,
1. The automatic liquid transferring mechanism has the advantages that firstly, the liquid transferring gun can be automatically controlled to drop liquid to be detected into the plurality of detection cavities of the detection dish, the experimental flow is more coherent and systematic in the automatic dropping process, the operation steps and time waste are reduced, the detection efficiency is improved, errors caused by manual operation are reduced, and the reliability of experimental results is improved.
2. According to the invention, the automatic conveying mechanism is arranged, so that the detection dish can be automatically conveyed into the automatic dripping mechanism from the driving plate, and the detection dish can be automatically conveyed onto the driving plate from the automatic dripping mechanism after the dripping operation is finished, thereby further improving the automation degree of the microbial fluorescence detection system and further improving the detection efficiency.
3. According to the invention, the sealing mechanism is arranged, after the driving plate drives the detection vessel to move into the machine body, a person can conveniently adjust the sealing plate to plug the detection hole, so that the detection hole can be sealed, and the practicability of the microbial fluorescence detection system is further improved.
4. The detection result is completed in less than half an hour, and the release time of the product is shortened by two to five days. Therefore, the storage space and the cost can be reduced, the inventory turnover is faster, the flexibility and the response capability are greater, and the customer requirements are met.
Drawings
FIG. 1 is a schematic diagram showing a three-dimensional structure of a fluorescence detection system for microorganisms according to the present invention;
FIG. 2 is a schematic diagram showing the structure of an automatic dripping mechanism of a fluorescence detection system for microorganisms according to the present invention;
FIG. 3 is a schematic view of a portion of an automatic drip mechanism of a fluorescence detection system for microorganisms according to the present invention;
FIG. 4 is a schematic diagram showing the disassembly of a partial structure of an automatic dripping mechanism of a fluorescence detection system for microorganisms according to the present invention;
FIG. 5 is a schematic diagram showing the structure of a detection vessel of a fluorescence detection system for microorganisms according to the present invention;
FIG. 6 is a schematic diagram of an automatic pipette of a fluorescence detection system for microorganisms according to the present invention;
FIG. 7 is a schematic diagram of an automatic conveying mechanism of a fluorescence detection system for microorganisms according to the present invention;
FIG. 8 is a schematic diagram showing a partial structure of an automatic conveying mechanism of a microorganism fluorescence detection system according to the present invention;
FIG. 9 is a schematic diagram of a carrier plate of a fluorescence detection system for microorganisms according to the present invention;
FIG. 10 is a schematic diagram showing the structure of a sealing mechanism of a fluorescence detection system for microorganisms according to the present invention;
Fig. 11 is a schematic diagram of a partial structure of a sealing mechanism of a fluorescence detection system for microorganisms according to the present invention.
The illustration is 1, the organism; 2, detection hole, 3, automatic dropping mechanism, 301, support plate, 302, servo motor, 303, sliding hole, 304, screw rod, 305, sliding block, 306, connecting plate, 307, fixing seat, 308, driving wheel, 309, motor seat, 310, driving motor, 311, conveying belt, 312, round hole, 313, cylinder, 314, automatic pipette, 315, positioning plate, 316, positioning rod, 317, fixing plate, 318, rectangular hole, 319, rectangular block, 320, detection dish, 321, detection cavity, 322, guide block, 4, automatic conveying mechanism, 401, welding plate, 402, limit plate, 403, motor, 404, driving reel, 405, driven reel, 406, belt, 407, threaded rod, 408, ear plate, 409, conveying frame, 410, guide rod, 411, carrying plate, 412, auxiliary plate, 413, first limit hole, 414, second limit hole, 415, limit block, 5, sealing mechanism, 501, rotating seat, 502, rotating shaft, 503, engagement plate, 504, sealing plate, 505, 507, auxiliary hole, 506, 509, rubber pad, 509, guide ring, 509, coil spring, 8, drive shaft, 7, fixing plate, 8, coil spring, and 9.
Detailed Description
In order that the above objects, features and advantages of the application will be more clearly understood, a further description of the application will be rendered by reference to the appended drawings and examples. It should be noted that, without conflict, the embodiments of the present application and features in the embodiments may be combined with each other.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced otherwise than as described herein, and therefore the present invention is not limited to the specific embodiments of the disclosure that follow.
Embodiment 1 as shown in fig. 1, the present invention provides a microorganism fluorescence detection system, comprising a machine body 1, a detection hole 2, a driving plate 6, a mounting plate 7, a holding bottle 8, a conduit 9 and an automatic liquid dropping mechanism 3. A driving device for automatically moving the driving plate 6 in and out of the detection hole 2 is installed in the machine body 1. The containing bottle 8 is used for containing fluorescent dye. The conduit 9 is used for conveying fluorescent dye from the bottle 8 to the detecting dish 320, and the conveying process is controlled by a conveying pump inside the machine body 1. The detection hole 2 is arranged on one side of the machine body 1, the driving plate 6 is arranged in the detection hole 2, the mounting plate 7 is arranged on the machine body 1, a plurality of containing bottles 8 are arranged on one side of the mounting plate 7, and one end of the guide pipe 9 is communicated with the containing bottles 8.
Wherein, automatic dropping mechanism 3 is located one side of organism 1, and one side that organism 1 kept away from backup pad 301 is equipped with automatic conveying mechanism 4, and one side that organism 1 is close to detection hole 2 is equipped with sealing mechanism 5.
The machine body 1 is also internally provided with a central control system for controlling the automatic dropping mechanism 3 and the automatic conveying mechanism 4, and the process and principle of the central control system for receiving signals and generating signals are similar to the existing common control system and are not important points to be protected in the invention, so that the process and principle are not explained in detail.
The specific arrangement and action of the automatic liquid dropping mechanism 3, the automatic conveying mechanism 4 and the sealing mechanism 5 will be described in detail below.
As shown in fig. 2 to 6, the automatic drip mechanism 3 includes a support plate 301, a detection dish 320, and an automatic pipette 314. The upper surface of the detection vessel 320 is provided with a plurality of detection cavities 321 for containing liquid to be detected, and the detection cavities 321 are uniformly distributed on the upper surface of the detection vessel 320. The lower surface of the detection dish 320 and the upper surface of the driving plate 6 are positioned in the same plane, so that the detection dish 320 can be prevented from being clamped in the conveying process. One side of the supporting plate 301 is fixedly connected with the machine body 1, and the supporting plate 301 plays a supporting role.
One side of the supporting plate 301 is fixedly connected with a servo motor 302, a sliding hole 303 is formed in the upper surface of the supporting plate 301, a sliding block 305 is slidably connected to the inner wall of the sliding hole 303, a screw rod 304 is arranged on the side surface of the sliding block 305 in a threaded manner, the output end of the servo motor 302 is fixedly connected with one end of the screw rod 304, and a connecting plate 306 is fixedly connected to the upper surface of the sliding block 305. The servo motor 302 is started to drive the screw rod 304 to rotate, the screw rod 304 drives the sliding block 305, the sliding block 305 moves along the inner wall of the sliding hole 303, the sliding block 305 drives the connecting plate 306, the connecting plate 306 drives the automatic pipetting gun 314 above the connecting plate, and the automatic pipetting gun 314 can automatically move transversely.
The both sides of connecting plate 306 are all fixedly connected with fixing base 307, and the inner wall of fixing base 307 rotates and is connected with drive wheel 308, and one side fixedly connected with motor cabinet 309 of one of them fixing base 307, the inner wall fixedly connected with driving motor 310 of motor cabinet 309, driving motor 310's output and one end fixedly connected with of one of them drive wheel 308, and the circular arc face transmission of two drive wheels 308 is connected with a conveyer belt 311. The upper surface of the detecting dish 320 is attached to the conveyor belt 311, so that liquid sputtering in the dropping process can be avoided. The surface of the conveyor belt 311 is provided with a round hole 312, the inner wall of the round hole 312 is fixedly connected with a cylinder 313, and an automatic pipette 314 is positioned inside the cylinder 313. The driving motor 310 is started to drive the driving wheel 308 to rotate, the driving wheel 308 drives the conveying belt 311 to operate, the conveying belt 311 drives the cylinder 313, the cylinder 313 drives the automatic pipetting gun 314 to move, and the automatic pipetting gun 314 can automatically move longitudinally.
In summary, the longitudinal movement and the lateral movement of the automatic pipetting gun 314 are mutually matched, so that the automatic pipetting gun 314 can be automatically adjusted to drop the liquid to be detected into each detection cavity 321 on the detection dish 320.
The outer wall of the cylinder 313 is fixedly connected with a locating plate 315, a locating rod 316 is arranged on the side face of the locating plate 315 in a sliding penetrating mode, fixing plates 317 are fixedly connected to two ends of the locating rod 316, and the two fixing plates 317 are respectively fixedly connected with one sides, away from each other, of the two fixing seats 307. When the conveyor belt 311 drives the cylinder 313 and the automatic pipetting gun 314 to move, the positioning plate 315 fixed on the cylinder 313 can move along the arc surface of the positioning rod 316, so that the stability of the cylinder 313 in the moving process of the automatic pipetting gun 314 is improved, the stability of the pipetting process is improved, and shaking of the automatic pipetting gun 314 and the cylinder 313 due to overhigh gravity centers is avoided.
The arc surface of the automatic pipetting gun 314 is fixedly connected with a plurality of rectangular blocks 319, a plurality of rectangular holes 318 are formed in the upper end of the cylinder 313, and the rectangular blocks 319 are attached to the inner walls of the rectangular holes 318. Before the dripping operation, the rectangular block 319 fixed on the automatic pipette 314 can be placed in the rectangular hole 318, so that the automatic pipette 314 can be quickly supported and positioned.
One side of the detecting dish 320 away from the driving plate 6 is fixedly connected with a guide block 322, and the guide block 322 is arranged into a wedge-shaped structure. By arranging the guide block 322 with a wedge-shaped structure on one side of the detection vessel 320, the process of moving the detection vessel 320 to the inside of the conveyor belt 311 can be smoother, and the phenomenon that the conveyor belt blocks the detection vessel 320 to cause the detection vessel to be incapable of moving can be avoided.
The whole automatic liquid dropping mechanism 3 has the advantages that the automatic liquid dropping mechanism is arranged to achieve two effects, namely, firstly, the liquid to be detected can be automatically dropped into the plurality of detection cavities 321 of the detection vessel 320 by the automatic liquid dropping gun, the experimental flow is more coherent and systematic in the automatic liquid dropping process, the operation steps and time waste are reduced, the detection efficiency is improved, errors caused by manual operation are reduced, and the reliability of experimental results is improved, secondly, the detection cavities 321 can be sealed and shielded by the conveying belt 311 in the liquid dropping process, and further, the sputtering of the detection liquid in the liquid dropping process can be avoided, so that the influence of sample loss can be facilitated, and the experimental environment pollution can be avoided.
As shown in fig. 7 to 9, the automatic conveying mechanism 4 includes a welding plate 401 and a bearing plate 411, one side of the welding plate 401 is fixedly connected with the machine body 1, one side of the welding plate 401 away from the machine body 1 is rotationally connected with a driving reel 404 and a driven reel 405, one side of the welding plate 401 away from the machine body 1 is fixedly connected with a limiting plate 402, one side of the limiting plate 402 is fixedly connected with a motor 403, an output end of the motor 403 is fixedly connected with the driving reel 404, the driving reel 404 is in transmission connection with an arc surface of the driven reel 405, a threaded rod 407 is rotationally penetrated on a side surface of the welding plate 401, one end of the threaded rod 407 is fixedly connected with the driven reel 405, an arc surface of the threaded rod 407 is in threaded connection with an ear plate 408, an upper surface of the ear plate 408 is fixedly connected with a conveying frame 409, and a section of the conveying frame 409 is in a shape of "C". The motor 403 is started to drive the driving reel 404 to rotate, the driving reel 404 drives the driven reel 405 to rotate by means of the belt 406, the driven reel 405 drives the threaded rod 407 to rotate, and the threaded rod 407 drives the conveying frame 409 to move, so that the conveying frame 409 is used for automatically pushing the detection dish 320.
One side of the bearing plate 411 is attached to the driving plate 6, the upper surface of the bearing plate 411 and the upper surface of the driving plate 6 are located in the same plane, the lower surface of the bearing plate 411 is fixedly connected with an auxiliary plate 412, and one side of the auxiliary plate 412 is fixedly connected with one side of the supporting plate 301 close to the driving plate 6. The carrier plate 411 can always support the detection dish 320 during the pushing process of the detection dish 320.
To sum up, when the detection dish 320 needs to be automatically pushed from the driving plate 6 to the position to be dripped, the motor 403 is started to drive the driving roller 404 to rotate, the driving roller 404 drives the driven roller 405 to rotate by means of the belt 406, the driven roller 405 drives the threaded rod 407 to rotate, the threaded rod 407 drives the conveying frame 409 to move, the conveying frame 409 pushes the detection dish 320 on the driving plate 6, so that the detection dish 320 sequentially moves to the inside of the conveying belt 311 along the surfaces of the driving plate 6 and the bearing plate 411, then dripping operation can be performed, after the dripping operation is completed, the motor 403 is started to drive the driving roller 404 to reversely rotate, the driving roller 404 drives the driven roller 405 to rotate by means of the belt 406, the driven roller 405 drives the threaded rod 407 to rotate, the threaded rod 407 drives the conveying frame 409 to move, and the conveying frame 409 pushes the detection dish 320 on the bearing plate 411, so that the detection dish 320 moves from the surface of the bearing plate 411 to the driving plate 6.
One side of the conveying frame 409 is slidably penetrated with a guide rod 410, one end of the short arm end of the guide rod 410 is fixedly connected with the welding plate 401, and when the threaded rod 407 drives the conveying frame 409 to move, the guide rod 410 can guide and limit the conveying frame 409, so that the shifting of the conveying frame 409 in the moving process can be avoided.
The upper surface of loading board 411 has seted up first spacing hole 413, and the second spacing hole 414 has been seted up to the upper surface of drive plate 6, and first spacing hole 413 is linked together with second spacing hole 414, and the inner wall sliding connection of first spacing hole 413 has stopper 415, stopper 415 and the lower fixed surface who detects dish 320 are connected, and when carriage 409 promotes to detect dish 320 and removes along the upper surface of loading board 411 or drive plate 6, the stopper 415 of fixing on detecting dish 320 can remove along the inner wall of first spacing hole 413 and second spacing hole 414 to can avoid detecting the removal process of dish 320 to take place the skew.
The effect that its whole automatic conveying mechanism 4 reached is, through setting up automatic conveying mechanism 4, can carry detection dish 320 to automatic dropping mechanism 3 by drive plate 6 automatically, and can carry detection dish 320 by automatic dropping mechanism 3 to on the drive plate 6 after the operation of dropping is accomplished automatically, has further improved microorganism fluorescence detection system's degree of automation, has further improved detection efficiency.
As shown in fig. 10 and 11, the sealing mechanism 5 includes a rotating seat 501, one side of the rotating seat 501 is fixedly connected with the machine body 1, the inner wall of the rotating seat 501 rotates to penetrate through a rotating shaft 502, an arc surface of the rotating shaft 502 is fixedly connected with a connecting plate 503, one side of the connecting plate 503 is fixedly connected with a sealing plate 504, an auxiliary hole 505 is formed in the side surface of the sealing plate 504, an arc hole 507 is formed in one side of the rotating seat 501, the center of the arc hole 507 is coaxial with the rotating axis of the rotating shaft 502, a fixing rod 508 is slidably connected with the inner wall of the arc hole 507, one end of the fixing rod 508 is fixedly connected with one side of the connecting plate 503, and an arc surface of the fixing rod 508 is in threaded connection with an adjusting ring 509. When the detection hole 2 is to be blocked by the sealing plate 504, the auxiliary hole 505 is pulled to drive the sealing plate 504, the sealing plate 504 drives the connecting plate 503 at this time, the connecting plate 503 drives the rotating shaft 502 to rotate along the inner wall of the rotating seat 501, and meanwhile, the fixing rod 508 on the connecting plate 503 slides along the inner wall of the arc-shaped hole 507, and after the detection hole 2 is blocked by the sealing plate 504, the adjusting ring 509 is rotated to abut against the rotating seat 501, so that the sealing operation of the detection hole 2 can be completed.
The rubber pad 510 is fixedly connected to one side of the adjusting ring 509, which is close to the rotating seat 501, and the diameter of the rubber pad 510 is larger than that of the adjusting ring 509, and the rubber pad 510 can improve the friction force of the adjusting ring 509, which is close to one side of the rotating seat 501, so that the effect that the adjusting ring 509 can be stably abutted with the rotating seat 501 by means of the rubber pad 510 is achieved.
The circular arc surface of pivot 502 is overlapped and is had coil spring 506, and coil spring 506's both ends respectively with linking board 503 and rotation seat 501 fixed connection, rotate the back of adjusting ring 509 and rotation seat 501 separation, coil spring 506 can automatic drive closing plate 504 to the direction rotation of keeping away from detection hole 2, has reached the effect that can open closing plate 504 fast.
The effect that its whole sealing mechanism 5 reached is, through setting up sealing mechanism 5, drives at drive plate 6 and detects the ware 320 and remove to inside organism 1, and the personnel can be convenient adjust closing plate 504 and plug up detection hole 2 to can seal up detection hole 2, thereby further improved microorganism fluorescence detecting system's practicality.
The whole working principle is that when the detection system is required to detect or quantify the microorganism, the following steps are required to be carried out:
S1, a precursor driving plate 6 moves from the inside of a detection hole 2, after a second limiting hole 414 on the driving plate 6 is aligned with a first limiting hole 413 on a bearing plate 411, the movement of the driving plate 6 is stopped, a motor 403 is restarted to drive a driving roller 404 to rotate, the driving roller 404 drives a driven roller 405 to rotate by a belt 406, the driven roller 405 drives a threaded rod 407 to rotate, the threaded rod 407 drives a conveying frame 409 to move, the conveying frame 409 pushes a detection dish 320 on the driving plate 6, and the detection dish 320 sequentially moves to the inside of a conveying belt 311 along the surfaces of the driving plate 6 and the bearing plate 411, so that the detection dish 320 can be automatically pushed to a liquid level to be dropped from the driving plate 6;
S2, after the detection vessel 320 is automatically pushed to the position to be dripped from the driving plate 6, the servo motor 302 is started to drive the screw rod 304 to rotate, the screw rod 304 drives the slide block 305 to enable the slide block 305 to move along the inner wall of the slide hole 303, the slide block 305 drives the connecting plate 306, the connecting plate 306 drives the automatic pipetting gun 314 above the slide block, the automatic pipetting gun 314 can automatically transversely move, the driving motor 310 is started to drive the driving wheel 308 to rotate, the driving wheel 308 drives the conveying belt 311 to operate, the conveying belt 311 drives the cylinder 313, the cylinder 313 drives the automatic pipetting gun 314 to move, the automatic pipetting gun 314 can automatically longitudinally move, and the longitudinal movement and the transverse movement of the automatic pipetting gun 314 are mutually matched, so that 50 mu l of to-be-detected liquid can be dripped into each detection cavity 321 on the detection vessel 320 by the automatic pipetting gun 314.
S3, after the drip operation is completed, the motor 403 is started to drive the driving roller 404 to reversely rotate, the driving roller 404 drives the driven roller 405 to rotate by means of the belt 406, the driven roller 405 drives the threaded rod 407 to rotate, the threaded rod 407 drives the conveying frame 409 to move, and the conveying frame 409 pushes the detecting dish 320 on the bearing plate 411, so that the detecting dish 320 moves from the surface of the bearing plate 411 to the driving plate 6.
S4, when the detection dish 320 moves onto the driving plate 6 from the surface of the bearing plate 411, the driving plate 6 is controlled to drive the detection dish 320 to enter the machine body 1, and a conveying pump in the machine body 1 is started to guide fluorescent dye into detection, so that detection operation can be performed, and detection can be completed within 27 minutes.
The present invention is not limited to the above embodiments, and any equivalent embodiments which can be changed or modified by the technical disclosure described above can be applied to other fields, but any simple modification, equivalent changes and modification to the above embodiments according to the technical matter of the present invention will still fall within the protection scope of the technical disclosure.