Disclosure of Invention
Based on the above problems, a first object of the present invention is to provide an application of a tannic acid metal complex in improving germination rate, seedling rate and promoting growth of rice seedlings.
Tannic acid, also known as tannic acid, molecular formula C76H52O46, english name TANNIC ACID, CAS numbers 1401-55-4.
The tannic acid metal complex is obtained by mixing tannic acid aqueous solution with zinc salt aqueous solution or manganese salt aqueous solution, stirring and reacting for 3 hours, wherein the molar ratio of tannic acid to zinc salt or manganese salt is 1:20-30.
As a preferable technical scheme of the invention, the zinc salt is Zn (CH3COO)2、ZnSO4、ZnCl2、Zn(NO3)2; the manganese salt is MnSO4、MnCl2、Mn(NO3)2).
More preferably, the zinc salt is Zn (CH3COO)2; the manganese salt is preferably MnSO4;
further, in the method for obtaining a tannic acid metal complex, the molar ratio of tannic acid to the zinc salt or manganese salt is preferably 1:27.5.
The tannic acid metal complex is used for seed treatment and has good effects of improving germination rate and seedling rate of rice seeds and promoting growth of rice seedlings.
The tannic acid metal complex can improve germination rate by 6% -16%, improve seedling rate by 0.7% -12% and increase rice plant height by 15.35% -81.16% under the treatment dose of 0.1-0.4 g/100 kg seeds.
Preferably, the tannic acid metal complex is used for rice seed treatment at a dosage of 0.3-0.4 g/100 kg seeds.
Wherein, the manganese tannate can improve the germination rate of the rice seeds by 14.0 to 16.0 percent, the seedling rate by 8.7 to 12.0 percent and the plant height by 69.17 to 81.16 percent under the seed treatment dosage of 0.3 to 0.4 g/100 kg seeds. The zinc tannate can improve the germination rate of rice seeds by 10.0 to 13.0 percent, the seedling rate by 4.0 to 11.3 percent and the plant height by 23.55 to 55.61 percent under the seed treatment dosage of 0.3 to 0.4 g/100 kg seeds.
The invention has the advantages that:
1. The tannic acid metal complex can improve the germination rate of rice seeds, promote the seedling formation of rice seeds and the growth of rice seedlings (improve the plant height of rice), thereby enhancing the quality of rice seedlings and ensuring the health of rice seedlings.
2. Tannic acid is friendly to the environment. The invention provides a new application of the compound, and the tannic acid metal complex can be used as a seed treatment agent for direct seeding of rice seeds.
Detailed Description
The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
In the following examples, references to "parts by mass" are to be understood in the art as "g", "kg", "mg", "μg", etc., in terms of weight.
The following examples and test examples relate to reagents disclosed below:
Tannic acid (96%, tianjin Sheen s Biochemical Co., ltd.), KCl (99.5%, national pharmaceutical Co., ltd.), mgSO4 (99%, tianjin Fuchen chemical Co., ltd.), zn (CH3COO)2 (98%, shanghai Michelin Biochemical Co., ltd.), caCl2 (96%, national pharmaceutical Co., ltd.), fe2(SO4)3 (96%, tianjin Sheen s Biochemical Co., ltd.), mnSO4 (99%, tianjin Sheen s Biochemical Co., ltd.).
The rice seeds are Song F17 and japonica type conventional rice.
Example 1 use of tannic acid Metal Complex for increasing germination Rate of Rice
1. Preparation of tannic acid metal complex
Dissolving tannic acid into deionized water to prepare a tannic acid solution of 0.012 mol/L, respectively dissolving KCl, caCl2、MgSO4、Zn(CH3COO)2、MnSO4 and Fe2(SO4)3 into deionized water to prepare a metal salt solution of 0.33 mol/L, mixing the tannic acid solution and the metal salt solution according to the volume ratio of 1:1, stirring for 3 hours, and drying to remove water after the reaction is completed to obtain the tannic acid metal complex.
2. Rice seed treatment
The preparation of the rice seed treating agent comprises weighing 5mL deionized water, mixing tannic acid, metal salt or tannic acid metal complex prepared in step one of 0.2-0.8 mg, adding 0.25 g film forming agent (styrene-acrylate emulsion, solid content 53% and film forming agent 11-1# of Beijing Yite agricultural technology popularization service Co., ltd.) respectively to obtain rice seed treating agent containing tannic acid, metal salt or tannic acid metal complex prepared in step one as effective component, and preparing control treating agent in parallel by replacing effective component with equal amount of water.
The seed treatment method comprises weighing 1g of the rice seed treatment agent or control treatment agent according to the effective component (tannic acid, metal salt, tannic acid metal complex or control treatment agent prepared in step one) of 0.1-0.4 g/100 kg seed (specific dosage is shown in table 1 and table 2), and mixing with 40g rice seed Song F17 to obtain coated rice seed.
3. Rice sowing and culturing method
A detection method of the germination rate of rice seeds in a germination test of a GB/T3543.4-1995 crop seed test procedure is adopted, tannic acid, metal salt or tannic acid metal complex is respectively arranged for treatment of the rice seeds serving as an effective component, a contrast treatment agent coating is used as contrast treatment, 25 rice seeds are placed in a 9 cm culture dish by a paper bed method, repetition is carried out for 4 times, 5 mL deionized water is evenly added into each dish, and dark and moisture-preserving culture is carried out in a 25 ℃ incubator.
4. Investigation method
After culturing 14 d, the germination number of each treatment was investigated and recorded, and the germination rate increase were calculated according to the following formula:
5. Test results
The rice seed germination rate measurement results after the treatment with the rice seed treatment agent containing tannic acid, metal salt or tannic acid metal complex prepared in the first step as an active ingredient are shown in tables 1 and 2. Compared with a control group, the MnSO4 can improve the germination rate of rice seeds by 10.0% -17.0% under the condition that the dosage of 0.1-0.4 g/100 kg seeds is used, the Fe2(SO4)3 can improve the germination rate of rice seeds by 8.0% -13.0% under the condition that the dosage of 0.1-0.4 g/100 kg seeds is used, the Zn (CH3COO)2 and KCl are used under the dosage of 0.2-0.4 g/100 kg seeds, the germination rate of rice seeds can be improved by 2.0% -14.0% and 2.0% -4.0%, and tannic acid has no promotion effect or insignificant effect on germination of rice seeds under the dosage of 0.1-0.4 g/100 kg seeds, the dosage of magnesium tannate, manganese tannate, iron tannate and zinc tannate are used under the dosage of 0.1-0.4 g/100 kg seeds, the germination rate of rice seeds can be improved by more than 6%, the maximum germination rate can be improved by 16.0% -16.0%, wherein the dosage of zinc tannate and zinc can be improved by 2.0% -14.0% -0.0% and 2.0% -4.0% respectively, and the optimal dosage of tannic acid can be improved by 6% -6.0% -6% and the optimal dosage of zinc can be improved by 0.0% -16% -0.0% and 6% of zinc can be improved.
TABLE 1 influence of tannic acid and Metal salt Single dose treatment on germination Rate of Rice seeds
TABLE 2 influence of tannic acid Metal Complex treatment on germination Rate of Rice seeds
Example 2 application of tannic acid Metal Complex in increasing seedling Rate of Rice
1. Preparation of tannic acid metal complex
The tannic acid metal complex was prepared in the same manner as in example 1.
2. Rice seed treatment
Preparation of rice seed treatment A seed treatment was prepared by mixing tannic acid, a metal salt and a tannic acid metal complex or a control treatment with a film former and water in a certain ratio, respectively, in the method of reference example 1.
Seed treatment method referring to example 1, rice seed treatment agent or control treatment agent containing tannic acid, metal salt or tannic acid metal complex prepared in step one as effective component is coated with rice seed according to the effective component amount of 0.1-0.4 g/100 kg seed, respectively.
3. Rice sowing and culturing method
The rice seed treatment agent treatment and the blank control treatment group which take tannic acid, metal salt or tannic acid metal complex prepared in the first step as effective components are arranged, 50 grains are sowed in each treatment, and the treatment is repeated for 3 times. Specifically, preparing nutrient soil according to the ratio of turf to vermiculite to chicken manure of 7:3:1, adding tap water, sowing coated rice seeds into the soil after reaching saturated water content, uniformly covering 1 cm thick soil on the surface layer, placing the soil in a greenhouse for culture, and watering once every 3 days. The temperature in the greenhouse is 25-30 ℃ in daytime, 13-15 ℃ in nighttime and the air humidity is 50-90%.
4. Investigation method
After 30 d of sowing, the number of seedlings formed by each treatment is investigated, the seedling forming rate and the growth of the seedling forming rate are counted, and the method is calculated according to the following formula:
5. Test results
The rice seeds were coated with the rice seed treatment agent comprising tannic acid, metal salt or tannic acid metal complex prepared in the first step as an active ingredient, and the effect results on the rice seedling rate are shown in tables 3 and 4. The MnSO4 can improve the seedling rate of rice seeds by 2.0-7.3% under the condition of the coating dosage of 0.1-0.4 g/100 kg seeds, the influence of other metal salts on the seedling rate of the seeds is unstable, and the phenomenon of inhibiting the rice seedlings occurs under individual concentration. The manganese tannate can improve the seedling rate by 6.0 to 12.0 percent under the coating dosage of 0.1 to 0.4 g/100 kg seeds, and especially can improve the seedling rate of 8.7 to 12.0 percent under the seed treatment dosage of 0.3 to 0.4 g/100 kg seeds. In addition, the zinc tannate can improve the seedling rate by 0.7-11.3%, and especially can improve the seedling rate of rice seeds by 10.0-13.0% under the seed treatment dosage of 0.3-0.4 g/100 kg seeds. Manganese tannate and zinc tannate perform optimally in promoting rice seeding and seedling formation. The magnesium tannate can improve the seedling rate by 1.3-7.3% under the coating dosage of 0.1-0.3 g/100 kg seeds, and other tannic acid metal complexes have unstable influence on rice seedling.
TABLE 3 influence of tannic acid and Metal salt Single dose treatment on the seedling rate of Rice seeds
TABLE 4 influence of tannic acid Metal Complex treatment on the seedling Rate of Rice seeds
Example 3 use of tannic acid Metal Complex for promoting growth of Rice seedlings
1. Preparation of tannic acid metal complex
The tannic acid metal complex was prepared in the same manner as in example 1.
2. Rice seed treatment
Preparation of rice seed treatment agent referring to the method of example 1, tannic acid, metal salt and tannic acid metal complex or equivalent amount of water are mixed with film forming agent and water in a certain ratio, respectively, to prepare seed treatment agent or control treatment agent.
Seed treatment method referring to example 1, rice seed treatment agent or control treatment agent containing tannic acid, metal salt or tannic acid metal complex prepared in step one as effective component is coated with rice seed according to the effective component amount of 0.1-0.4 g/100 kg seed, respectively.
3. The rice seeding culture method comprises the steps of setting a rice seed treating agent treatment and a blank control treatment group which take tannic acid, metal salt or tannic acid metal complex prepared in the first step as effective components, and sowing 50 grains for each treatment, wherein the steps are repeated for 3 times. Specifically, the seeding and seedling growing conditions were the same as in example 2.
4. The investigation method comprises the following steps of investigating the plant height of the rice after 30 d of sowing, counting the growth rate of each treated plant height, and calculating according to the following formula:
5. Test results
The rice seeds were coated with the rice seed treatment agent comprising tannic acid, metal salt or tannic acid metal complex prepared in the first step as an active ingredient, and the effect results on the plant height of rice seedlings are shown in tables 5 and 6. The tannic acid single dose has obvious growth promoting effect on rice plant height under the dosage of 0.2-0.4 g/100 kg seeds, but has inhibiting effect on seedling growth under the dosage of 0.1g/100 kg seeds. In contrast to the effect of different metal salts on rice seedling growth, the effect of MnSO4 and Zn (CH3COO)2 can respectively increase the plant height by 24.45% -68.69% and 5.96% -23.73% and show growth promotion effect) is different under the dosage of 0.1-0.4 g/100 kg seeds, the effect of manganese tannate and zinc tannate can respectively increase the rice plant height by 67.17% -81.16% and 15.35% -55.61%, especially under the dosage of 0.3-0.4 g/100 kg seeds, the effect of 69.17% -81.16% and 23.55% -55.61% of rice seedling plant height can be improved, in addition, iron tannate can increase the rice plant height by 18.86% -38.03%, and the effect of manganese tannate, zinc tannate and iron tannate can show seedling growth promotion effect under the dosage of partial dosage of tannic acid metal complex can not stabilize the growth promotion effect of rice seedling.
TABLE 5 influence of tannic acid and Metal salt Single dose treatment on plant height of Rice
TABLE 6 influence of tannic acid and Metal salt Single dose treatment on plant height of Rice
The invention discovers that tannic acid metal complex is prepared by mixing tannic acid solution and zinc salt or manganese salt solution and stirring and reacting for 3 hours, wherein the molar ratio of tannic acid to zinc salt or manganese salt is 1:20-30, and the tannic acid metal complex is used for seed treatment under the seed dosage of 0.1-0.4 g/100 kg and has good effects of improving the germination rate and seedling rate of rice seeds and promoting the growth of rice seedlings (improving the plant height of rice).
The research result of the invention shows that the manganese tannate can improve the germination rate of rice seeds by 6.0-16.0%, the seedling rate by 6.0-12.0% and the plant height by 69.17-81.16% under the seed treatment dosage of 0.1-0.4 g/100 kg seeds, and the zinc tannate can improve the germination rate of rice seeds by 6.0-13.0%, the seedling rate by 0.7-11.3% and the plant height by 15.35-55.61% under the seed treatment dosage of 0.1-0.4 g/100 kg seeds. In particular, the promoting effect on the sprouting, the seedling and the growth of the seedlings of the rice is optimal under the seed treatment dosage of 0.3-0.4 g/100 kg seeds. The manganese tannate can promote the germination rate of rice seeds by 14.0% -16.0%, the seedling rate by 8.7% -12.0% and the plant height by 69.17% -81.16%, the zinc tannate can promote the germination rate of rice seeds by 10.0% -13.0%, the seedling rate by 4.0% -11.3% and the plant height by 23.55% -55.61%. The tannic acid is environment-friendly, and the invention provides a new application of the compounds, and the tannic acid metal complex can be used as a seed treatment agent for direct seeding of rice seeds.
While the invention has been described in detail in the foregoing general description, embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.