技术领域Technical Field
本发明属于化妆品技术领域，具体涉及一种除螨剂、其制备方法及应用。The present invention belongs to the technical field of cosmetics, and in particular relates to an anti-mite agent, a preparation method and application thereof.
背景技术Background Art
螨虫和微生物一样，在人类生活环境中无处不在，且无时无刻不在危害着人类的身体健康。螨虫广泛分布于居室的阴暗角落、地毯、床垫、枕头、沙发、空调、凉席等处，其尸体、分泌物和代谢物等吸入人的呼吸道或接触皮肤都容易引起流鼻涕、咳嗽、气喘等症状。Mites, like microorganisms, are everywhere in the human living environment and are harmful to human health all the time. Mites are widely distributed in dark corners of the room, carpets, mattresses, pillows, sofas, air conditioners, cool mats, etc. Their bodies, secretions and metabolites can easily cause runny nose, cough, asthma and other symptoms when inhaled into the human respiratory tract or contacted with the skin.
目前，市场中现有的除螨方法主要分为物理方法和化学方法两种，物理方法就是通过使用除螨仪、空气净化器等设备；经常清洗或太阳暴晒等方法，这些方法虽然有着一定的效果，但都比较费时费力且效果不佳。如：空气净化器耗时长且费电，清洗或太阳暴晒也很费时费力；市面上除螨仪所使用的紫外线灯管多为6～15W，功率较小，需长时间照射才有效果，虽然除螨仪上的拍打器有着较高频次的拍打，但幅度太小，对于爪子呈勾状的螨虫来说，并没有很大的除螨作用。化学方法主要是通过使用一些对螨虫有害或带有厌恶气味的物质来达到杀死或驱赶的功效，但市面上很多都是使用的化学合成的原料，会对人体健康带来一定程度的危害。At present, the existing mite removal methods in the market are mainly divided into physical methods and chemical methods. The physical method is to use mite removal devices, air purifiers and other equipment; frequent cleaning or sun exposure and other methods. Although these methods have certain effects, they are time-consuming and labor-intensive and have poor results. For example: air purifiers are time-consuming and power-consuming, and cleaning or sun exposure is also time-consuming and labor-intensive; the ultraviolet lamps used in mite removal devices on the market are mostly 6-15W, with low power and require long-term exposure to be effective. Although the flapper on the mite removal device has a higher frequency of flapping, the amplitude is too small, and for mites with hooked claws, it does not have a great mite removal effect. The chemical method mainly uses some substances that are harmful to mites or have a disgusting odor to achieve the effect of killing or repelling, but many of the raw materials used in the market are chemically synthesized, which will bring a certain degree of harm to human health.
中国授权专利CN109673682B公开了一种天然抗菌除螨剂的制备方法，其技术要点包括如下操作步骤：按比例称量山鸡椒、苦参、丁香花或者百里香后放入提取罐内，加入油溶剂进行外循环提取，提取后将油提取液经干燥器输入到搅拌罐内，然后在搅拌罐中加入表面活性剂，同时提取罐中加入纯化水进行蒸馏提取，蒸馏液经冷凝器冷凝后经油水分离器分离精油和水，其中精油经干燥器输入搅拌罐中混合，水回流入提取罐，提取后将水提取液泵入精制器，经精制器脱色、除杂后进入缓冲罐，然后通过流量计、分布器滴入搅拌罐，得到天然抗菌除螨剂。本发明制作得到的天然抗菌除螨剂稳定性好、水溶性好、功效强，同时还提高了天然活性成分的综合提取率，缩短周期，降低了成本。China's authorized patent CN109673682B discloses a method for preparing a natural antibacterial and mite-removing agent, and its technical key points include the following operating steps: weighing Litsea cubeba, Sophora flavescens, Clove or Thyme in proportion and putting them into an extraction tank, adding an oil solvent for external circulation extraction, and after extraction, inputting the oil extract into a stirring tank through a dryer, and then adding a surfactant to the stirring tank, and adding purified water to the extraction tank for distillation extraction, and the distillate is condensed by a condenser and separated into essential oil and water by an oil-water separator, wherein the essential oil is input into the stirring tank through a dryer for mixing, and the water is refluxed into the extraction tank, and after extraction, the water extract is pumped into a refiner, and after decolorization and impurity removal by the refiner, it enters a buffer tank, and then drips into the stirring tank through a flow meter and a distributor to obtain a natural antibacterial and mite-removing agent. The natural antibacterial and mite-removing agent prepared by the present invention has good stability, good water solubility, and strong efficacy, and at the same time, it also improves the comprehensive extraction rate of natural active ingredients, shortens the cycle, and reduces costs.
再例如，中国授权专利CN111903720B公开了一种植物抗菌除螨剂及其制备方法。其中，植物抗菌除螨剂的制备方法包括：将乙醇、表面活性剂、二氯苄醇、麝香草酚、植物精油和丙二醇混合，完全溶解后，制得植物抗菌除螨剂；其中，所述表面活性剂由聚氧乙烯辛基苯酚醚和月桂基硫酸铵组成，所述植物精油由冬青精油、香茅精油、天竺葵精油和茶树精油组成。本发明将四种植物精油、丙二醇、聚氧乙烯辛基苯酚醚、月桂基硫酸铵、乙醇、麝香草酚以及二氯苄醇进行复配之后可协同增效，增强植物抗菌除螨剂整体的抑菌、除螨效果，并能提高植物抗菌除螨剂体系的稳定性，防止出现析出沉淀等现象。For another example, Chinese authorized patent CN111903720B discloses a plant antibacterial and mite removal agent and a preparation method thereof. The preparation method of the plant antibacterial and mite removal agent includes: mixing ethanol, a surfactant, dichlorobenzyl alcohol, thymol, plant essential oil and propylene glycol, and preparing the plant antibacterial and mite removal agent after complete dissolution; wherein the surfactant is composed of polyoxyethylene octylphenol ether and ammonium lauryl sulfate, and the plant essential oil is composed of holly essential oil, citronella essential oil, geranium essential oil and tea tree essential oil. The present invention can synergize and enhance the overall antibacterial and mite removal effects of the plant antibacterial and mite removal agent after compounding four plant essential oils, propylene glycol, polyoxyethylene octylphenol ether, ammonium lauryl sulfate, ethanol, thymol and dichlorobenzyl alcohol, and can improve the stability of the plant antibacterial and mite removal agent system to prevent precipitation and other phenomena.
然而，上述天然的除螨剂成分较为复杂，质量控制存在一定的难度。因此，申请人试图基于对活性多肽的研究，提出了一种成分清析、除螨效果好的除螨剂。However, the above-mentioned natural mite removal agents have relatively complex ingredients and quality control is difficult. Therefore, the applicant attempted to propose a mite removal agent with clear ingredients and good mite removal effect based on the research of active polypeptides.
发明内容Summary of the invention
基于上述原因，本发明旨在提供一种除螨剂、其制备方法及应用。具体而言，为了实现本发明的目的，本发明拟采用如下的技术方案：Based on the above reasons, the present invention aims to provide a mite remover, a preparation method and application thereof. Specifically, in order to achieve the purpose of the present invention, the present invention intends to adopt the following technical solutions:
本发明一方面涉及一种除螨剂，其包括活性成分以及辅料，其中，活性成分为氨基酸序列为EQKM的短肽，所述辅料包括透明质酸。One aspect of the present invention relates to a mite remover, which comprises an active ingredient and an auxiliary material, wherein the active ingredient is a short peptide with an amino acid sequence of EQKM, and the auxiliary material comprises hyaluronic acid.
在本发明的一个优选实施方式中，所述除螨剂为液体制剂，所述液体制剂为水性制剂。In a preferred embodiment of the present invention, the acaricide is a liquid preparation, and the liquid preparation is an aqueous preparation.
在本发明的一个优选实施方式中，所述活性成分与透明质酸的重量比例为10:2-5；优选为10:2.5-3.5；特别优选为10:3。In a preferred embodiment of the present invention, the weight ratio of the active ingredient to hyaluronic acid is 10:2-5; preferably 10:2.5-3.5; particularly preferably 10:3.
在本发明的一个优选实施方式中，所述活性成分在除螨剂中的比例为0.2-0.5wt％。In a preferred embodiment of the present invention, the proportion of the active ingredient in the acaricide is 0.2-0.5 wt %.
在本发明的一个优选实施方式中，所述透明质酸在除螨剂中的比例为0.8-1.5wt％。In a preferred embodiment of the present invention, the proportion of hyaluronic acid in the acaricide is 0.8-1.5 wt %.
在本发明的一个优选实施方式中，所述除螨剂还包括香精。In a preferred embodiment of the present invention, the acaricide further comprises fragrance.
本发明另一方面还涉及上述除螨剂的制备方法，其包括如下步骤：Another aspect of the present invention also relates to a method for preparing the above-mentioned acaricide, which comprises the following steps:
(1)将透明质酸溶解在去离子水中，升温至50℃保温搅拌至溶解均匀；(1) Dissolve hyaluronic acid in deionized water, heat to 50°C, keep warm and stir until dissolved evenly;
(2)将序列为EQKM的短肽加入上述透明质酸溶液中，搅拌至溶解之后降温至室温；(2) adding a short peptide with the sequence EQKM to the above hyaluronic acid solution, stirring until dissolved, and then cooling to room temperature;
(3)调节pH值至6.5-7.2即得。(3) Adjust the pH value to 6.5-7.2.
本发明另一方面还涉及上述除螨剂的应用，所述除螨剂用于制备除螨剂；优选的，所述除螨剂为化妆品，用于去除面部的螨虫。Another aspect of the present invention also relates to the application of the above-mentioned demitogen, which is used for preparing demitogen; preferably, the demitogen is a cosmetic used for removing facial mites.
有益效果Beneficial Effects
本发明的除螨剂通过配合使用EQKM的短肽和透明质酸，能够显著提高除螨效果，并且本发明的除螨剂由天然原料制成，可以作为化妆品直接用于人体面部以去除面部的螨虫，同时透明质酸本身具有保湿作用。The mite remover of the present invention can significantly improve the mite removal effect by using the EQKM short peptide and hyaluronic acid in combination. The mite remover of the present invention is made of natural raw materials and can be directly used as a cosmetic on the human face to remove facial mites. At the same time, hyaluronic acid itself has a moisturizing effect.
具体实施方式DETAILED DESCRIPTION
为了进一步理解本发明，下面将结合本发明实施例，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。In order to further understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
如无特殊说明，本发明实施例中所涉及的试剂均为市售产品，均可以通过商业渠道购买获得。Unless otherwise specified, the reagents involved in the embodiments of the present invention are all commercially available products and can be purchased through commercial channels.
实施例1：制备除螨剂Example 1: Preparation of acaricide
1、将1g透明质酸溶解在100g去离子水中，升温至50℃保温搅拌至溶解均匀；1. Dissolve 1g hyaluronic acid in 100g deionized water, heat to 50℃ and stir until dissolved evenly;
2、将0.2g序列为EQKM的短肽加入上述透明质酸溶液中，搅拌至溶解之后降温至室温；2. Add 0.2 g of the short peptide with the sequence of EQKM to the above hyaluronic acid solution, stir until dissolved and then cool to room temperature;
3、调节pH值至6.8即得。3. Adjust the pH value to 6.8.
实施例2：制备除螨剂Example 2: Preparation of acaricide
1、将1.5g透明质酸溶解在100g去离子水中，升温至50℃保温搅拌至溶解均匀；1. Dissolve 1.5g hyaluronic acid in 100g deionized water, heat to 50℃ and stir until dissolved evenly;
2、将0.2g序列为EQKM的短肽加入上述透明质酸溶液中，搅拌至溶解之后降温至室温；2. Add 0.2 g of the short peptide with the sequence of EQKM to the above hyaluronic acid solution, stir until dissolved and then cool to room temperature;
3、调节pH值至6.8即得。3. Adjust the pH value to 6.8.
实施例3：制备除螨剂Example 3: Preparation of acaricide
1、将1g透明质酸溶解在100g去离子水中，升温至50℃保温搅拌至溶解均匀；1. Dissolve 1g hyaluronic acid in 100g deionized water, heat to 50℃ and stir until dissolved evenly;
2、将0.3g序列为EQKM的短肽加入上述透明质酸溶液中，搅拌至溶解之后降温至室温；2. Add 0.3 g of the short peptide with the sequence of EQKM to the above hyaluronic acid solution, stir until dissolved and then cool to room temperature;
3、调节pH值至6.8即得。3. Adjust the pH value to 6.8.
实施例4：制备除螨剂Example 4: Preparation of acaricide
1、将1g透明质酸溶解在100g去离子水中，升温至50℃保温搅拌至溶解均匀；1. Dissolve 1g hyaluronic acid in 100g deionized water, heat to 50℃ and stir until dissolved evenly;
2、将0.4g序列为EQKM的短肽加入上述透明质酸溶液中，搅拌至溶解之后降温至室温；2. Add 0.4 g of the short peptide with the sequence of EQKM to the above hyaluronic acid solution, stir until dissolved and then cool to room temperature;
3、调节pH值至6.8即得。3. Adjust the pH value to 6.8.
比较例1：Comparative Example 1:
与实施例1相同，区别在于采用EQKS代替EQKM。The same as Example 1, except that EQKS is used instead of EQKM.
比较例2：Comparative Example 2:
与实施例1相同，区别在于采用海藻酸钠代替透明质酸。The same as Example 1, except that sodium alginate is used instead of hyaluronic acid.
实施例5：除螨剂灭螨活性试验Example 5: Test on the activity of acaricides
测试方法参考农业部灭螨测试标准方法：NY/T 1151.2-2006。The test method refers to the Ministry of Agriculture's standard method for mite control testing: NY/T 1151.2-2006.
实验设备与材料：恒温恒湿培养箱(温度(25±1)℃、湿度70％-80％)有盖容器(约30cm×25cm×5cm)、烧杯(150mL)、体视显微镜、一次性平皿、白油凡士林混合物(1:1)、饱和食盐水、粉尘螨成螨、螨虫饲料、螨虫计数工具(计数器，解剖针，毛笔)。Experimental equipment and materials: constant temperature and humidity incubator (temperature (25±1)℃, humidity 70%-80%), covered container (about 30cm×25cm×5cm), beaker (150mL), stereo microscope, disposable plate, white oil and vaseline mixture (1:1), saturated salt water, adult dust mites, mite feed, and mite counting tools (counter, dissecting needle, brush).
实验操作：取一次性平皿，平皿内加入5g待测样品，每个平皿内壁上缘均匀涂抹白衣凡士林混合物。每个平皿中心加入300只螨虫，30min后在平皿中心放入螨虫饲料0.05g。置于恒温恒湿培养箱内，2小时后，取出平皿在体视显微镜下检查记录死亡螨虫数目，计算除螨率。Experimental operation: Take a disposable plate, add 5g of the sample to be tested into the plate, and evenly apply the white vaseline mixture on the upper edge of the inner wall of each plate. Add 300 mites to the center of each plate, and after 30 minutes, put 0.05g of mite feed in the center of the plate. Place it in a constant temperature and humidity incubator. After 2 hours, take out the plate and check under a stereo microscope to record the number of dead mites and calculate the mite removal rate.
除螨率计算：除螨率＝死亡螨虫数/实验螨虫数*100。Calculation of mite removal rate: mite removal rate = number of dead mites/number of experimental mites*100.
测试结果如表1所示The test results are shown in Table 1
表1.除螨喷雾灭螨测试实验结果：Table 1. Test results of mite removal spray:
上述实验结果表明，本发明的实施例1-4对于螨虫的杀灭率为55％以上，高于比较例1-2。特别是实施例3，其通过采用特定比例的透明质酸和短肽EQKM，可以显著提高除螨率，为本发明最优选的实施例。The above experimental results show that the killing rate of mites in Examples 1-4 of the present invention is more than 55%, which is higher than that in Comparative Examples 1-2. In particular, Example 3, which uses a specific ratio of hyaluronic acid and short peptide EQKM to significantly improve the mite removal rate, is the most preferred embodiment of the present invention.
以上描述了本发明优选实施方式，然其并非用以限定本发明。本领域技术人员对在此公开的实施方案可进行并不偏离本发明范畴和精神的改进和变化。The above describes the preferred embodiments of the present invention, but it is not intended to limit the present invention. Those skilled in the art may make improvements and changes to the embodiments disclosed herein without departing from the scope and spirit of the present invention.
| Application Number | Priority Date | Filing Date | Title |
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| CN202410770502.6ACN118766789A (en) | 2024-06-14 | 2024-06-14 | A kind of mite removal agent, preparation method and application thereof |
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| CN202410770502.6ACN118766789A (en) | 2024-06-14 | 2024-06-14 | A kind of mite removal agent, preparation method and application thereof |
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| CN104093415A (en)* | 2011-10-24 | 2014-10-08 | 哈洛齐梅公司 | Companion diagnostic for anti-hyaluronan agent therapy and methods of use thereof |
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