技术领域Technical Field
本发明涉及生物检测技术领域，具体涉及一种核酸提取系统。The present invention relates to the technical field of biological detection, and in particular to a nucleic acid extraction system.
背景技术Background technique
核酸检测技术具有灵敏度高和准确度高的显著优势，并可根据检测方法的优化、靶标基因的筛选、以及引物探针的设计，实现种/属/型、以及片段/序列/位点等不同分辨度的鉴别分析。核酸检测技术广泛应用于临床检验、疾控应急和畜牧兽医等领域。Nucleic acid detection technology has the significant advantages of high sensitivity and high accuracy, and can achieve different resolutions of species/genus/type, fragment/sequence/site, etc. according to the optimization of detection methods, screening of target genes, and design of primers and probes. Nucleic acid detection technology is widely used in clinical testing, disease control and emergency response, animal husbandry and veterinary medicine, etc.
核酸检测流程一般包括：核酸提取与核酸分析。核酸提取技术是核酸提取、纯化和富集的重要步骤，是分析敏感性、准确性和特异性的核心支撑。核酸提取方法包括：膜柱法和磁珠法。但是，无论是膜柱法，还是磁珠法，由于在核酸提取的过程中，试剂种类繁多、流程较繁琐以及操作要求较高，严重制约核酸提取全系统的整合、封闭化、自动化和简便化。The nucleic acid detection process generally includes: nucleic acid extraction and nucleic acid analysis. Nucleic acid extraction technology is an important step in nucleic acid extraction, purification and enrichment, and is the core support for analytical sensitivity, accuracy and specificity. Nucleic acid extraction methods include: membrane column method and magnetic bead method. However, whether it is the membrane column method or the magnetic bead method, due to the wide variety of reagents, complicated procedures and high operating requirements in the process of nucleic acid extraction, the integration, closure, automation and simplification of the entire nucleic acid extraction system are seriously restricted.
发明内容Summary of the invention
因此，本发明要解决的技术问题在于克服在核酸提取的过程中，试剂种类繁多、流程较繁琐以及操作要求较高，严重制约核酸提取全系统的整合、封闭化、自动化和简便化的缺陷。Therefore, the technical problem to be solved by the present invention is to overcome the defects in the process of nucleic acid extraction, such as a wide variety of reagents, complicated procedures and high operating requirements, which seriously restrict the integration, closure, automation and simplification of the entire nucleic acid extraction system.
为了解决上述问题，本发明提供一种核酸提取系统，包括：In order to solve the above problems, the present invention provides a nucleic acid extraction system, comprising:
试剂仓，设有一个样本洗脱腔和至少两个试剂承载腔；在所述样本洗脱腔和试剂承载腔内均滑动设有活塞；在所述样本洗脱腔和试剂承载腔的一端分别具有出口；在所述样本洗脱腔上设有进样口；The reagent compartment is provided with a sample elution chamber and at least two reagent carrying chambers; pistons are slidably arranged in the sample elution chamber and the reagent carrying chamber; outlets are respectively arranged at one end of the sample elution chamber and the reagent carrying chamber; and a sample inlet is arranged on the sample elution chamber;
提取操作组件，与所述试剂仓配合，设有反应腔和核酸出口，所述反应腔与核酸出口之间设有第四微阀；在所述提取操作组件上设有与所述样本洗脱腔和试剂承载腔的出口对接的若干试剂入口，若干所述试剂入口之间通过内部通道连通；至少部分所述内部通道与所述反应腔连通，所述反应腔内设有核酸捕获物；An extraction operation component cooperates with the reagent compartment, is provided with a reaction chamber and a nucleic acid outlet, and a fourth microvalve is provided between the reaction chamber and the nucleic acid outlet; the extraction operation component is provided with a plurality of reagent inlets docked with the outlets of the sample elution chamber and the reagent carrying chamber, and the plurality of reagent inlets are connected through internal channels; at least part of the internal channels is connected with the reaction chamber, and a nucleic acid capture object is provided in the reaction chamber;
在使用时，通过不同活塞的滑动升降，使得相应的试剂进入样本洗脱腔、试剂承载腔和反应腔内，在所述反应腔内通过核酸捕获物捕获核酸，并通过核酸出口排出至核酸分析端。When in use, the corresponding reagents enter the sample elution chamber, the reagent carrying chamber and the reaction chamber through the sliding and lifting of different pistons, and the nucleic acid is captured by the nucleic acid capture object in the reaction chamber and discharged to the nucleic acid analysis end through the nucleic acid outlet.
可选地，所述提取操作组件包括：依次贴合设置的弹性密封膜、流道模块和底板；在所述底板内设有反应腔，在所述流道模块内设有内部通道。Optionally, the extraction operation component includes: an elastic sealing membrane, a flow channel module and a bottom plate which are sequentially attached to each other; a reaction chamber is provided in the bottom plate, and an internal channel is provided in the flow channel module.
可选地，还包括：Optionally, it also includes:
外壳，所述试剂仓与所述外壳连接，且所述外壳压附在所述弹性密封膜上。The reagent chamber is connected to the shell, and the shell is pressed onto the elastic sealing film.
可选地，在所述外壳上设有沿竖直方向的控制通道；Optionally, a control channel along the vertical direction is provided on the housing;
所述第四微阀包括：The fourth microvalve comprises:
控制腔室，设置于所述提取操作组件内；所述控制通道与所述控制腔室相对应；A control chamber is disposed in the extraction operation assembly; the control channel corresponds to the control chamber;
压杆，适于在所述控制通道中滑动上升或下降，以分别放松或推压所述弹性密封膜至控制腔室，进而分别连通或断开所连接的管路。The pressure rod is suitable for sliding up or down in the control channel to respectively loosen or push the elastic sealing membrane to the control chamber, thereby respectively connecting or disconnecting the connected pipeline.
可选地，所述试剂仓滑动连接于所述外壳内；在所述样本洗脱腔和试剂承载腔的出口连接有针头，所述试剂仓在所述外壳内通过朝向所述提取操作组件滑动，以使所述样本洗脱腔和试剂承载腔的出口与所述提取操作组件的试剂入口连通。Optionally, the reagent chamber is slidably connected to the outer shell; a needle is connected to the outlet of the sample elution chamber and the reagent carrying chamber, and the reagent chamber slides toward the extraction operation component in the outer shell to connect the outlet of the sample elution chamber and the reagent carrying chamber with the reagent inlet of the extraction operation component.
可选地，还包括：Optionally, it also includes:
弹性密封垫，设置于所述外壳内的试剂仓的下方，所述样本洗脱腔和试剂承载腔的出口的针头插入所述弹性密封垫内，以密封所述样本洗脱腔和试剂承载腔，使用时，所述试剂仓在所述外壳内通过朝向所述提取操作组件滑动，以使所述针头贯穿所述弹性密封垫。An elastic sealing gasket is arranged below the reagent chamber in the shell, and the needles at the outlets of the sample elution chamber and the reagent carrying chamber are inserted into the elastic sealing gasket to seal the sample elution chamber and the reagent carrying chamber. When in use, the reagent chamber slides toward the extraction operation component in the shell to allow the needle to penetrate the elastic sealing gasket.
可选地，在所述提取操作组件内还设有废液腔和排气口，所述排气口与废液腔之间设有第二微阀，所述废液腔与反应腔之间设有第三微阀；在所述废液腔内置有吸水材料，所述吸水材料适于吸取废液。Optionally, a waste liquid chamber and an exhaust port are further provided in the extraction operation component, a second microvalve is provided between the exhaust port and the waste liquid chamber, and a third microvalve is provided between the waste liquid chamber and the reaction chamber; a water-absorbing material is built into the waste liquid chamber, and the water-absorbing material is suitable for absorbing waste liquid.
可选地，所述试剂承载腔包括：裂解腔，所述裂解腔与废液腔通过蛇形防反流流道连通；在所述裂解腔与反应腔之间的内部通道上设有第一微阀。Optionally, the reagent carrying chamber includes: a lysis chamber, the lysis chamber is connected to the waste liquid chamber through a serpentine anti-backflow channel; and a first microvalve is provided on the internal channel between the lysis chamber and the reaction chamber.
可选地，所述试剂承载腔包括：裂解腔、清洗腔和洗脱液腔，所述清洗腔包括第一清洗液腔和第二清洗液腔；在所述裂解腔内存储有裂解液，在所述第一清洗液腔内存储有第一清洗液，在所述第二清洗液腔内存储有第二清洗液，所述洗脱液腔内存储有洗脱液。Optionally, the reagent carrying chamber includes: a lysis chamber, a cleaning chamber and an elution liquid chamber, the cleaning chamber includes a first cleaning liquid chamber and a second cleaning liquid chamber; a lysis liquid is stored in the lysis chamber, a first cleaning liquid is stored in the first cleaning liquid chamber, a second cleaning liquid is stored in the second cleaning liquid chamber, and an elution liquid is stored in the elution liquid chamber.
可选地，所述核酸捕获物为结合试剂和磁珠，在所述反应腔的底部设有电磁铁。Optionally, the nucleic acid capture object is a binding reagent and magnetic beads, and an electromagnet is provided at the bottom of the reaction chamber.
可选地，所述试剂承载腔包括：气动腔；在所述气动腔内存储有空气；使用时，通过下压所述气动腔的活塞，用于驱动所述反应腔内的核酸流至核酸出口。Optionally, the reagent carrying chamber includes: a pneumatic chamber; air is stored in the pneumatic chamber; when in use, the piston of the pneumatic chamber is pressed down to drive the nucleic acid in the reaction chamber to flow to the nucleic acid outlet.
可选地，所述核酸捕获物为结合试剂和富集膜。Optionally, the nucleic acid capture objects are binding reagents and enrichment membranes.
可选地，所述试剂承载腔包括：裂解腔和乙醇腔，所述裂解腔内设有消化缓冲液，在所述乙醇腔内存储有乙醇，在所述样本洗脱腔内存有蛋白酶k；使用时，首先推动所述裂解腔内的活塞，将所述消化缓冲液推动至所述样本洗脱腔内混合，然后将混合液推动至乙醇腔内。Optionally, the reagent carrying chamber includes: a lysis chamber and an ethanol chamber, the lysis chamber is provided with a digestion buffer, the ethanol chamber is stored with ethanol, and the sample elution chamber contains proteinase K; when in use, first push the piston in the lysis chamber to push the digestion buffer into the sample elution chamber for mixing, and then push the mixed liquid into the ethanol chamber.
本发明的上述技术方案相比现有技术具有以下优点：The above technical solution of the present invention has the following advantages compared with the prior art:
1.本发明提供的核酸提取系统，包括：试剂仓，设有一个样本洗脱腔和至少两个试剂承载腔；在所述样本洗脱腔和试剂承载腔内均滑动设有活塞；在所述样本洗脱腔和试剂承载腔的一端分别具有出口；在所述样本洗脱腔上设有进样口；提取操作组件，与所述试剂仓配合，设有反应腔和核酸出口，所述反应腔与核酸出口之间设有第四微阀；在所述提取操作组件上设有与所述样本洗脱腔和试剂承载腔的出口对接的若干试剂入口，若干所述试剂入口之间通过内部通道连通；至少部分所述内部通道与所述反应腔连通，所述反应腔内设有核酸捕获物；在使用时，通过不同活塞的滑动升降，使得相应的试剂进入样本洗脱腔、试剂承载腔和反应腔内，在所述反应腔内通过核酸捕获物捕获核酸，并通过核酸出口排出至核酸分析端；本申请采用上述技术方案，通过不同活塞的滑动升降实现封闭系统内的气压平衡和试剂的流动；最后，将提取后的核酸通过核酸出口注入下游各种核酸分析端。本申请技术方案提供的通用型的自动化核酸提取方案，依托核酸提取的全封闭化、全流程一体化和自动化，可为多样化的核酸分析端提供有效支持。1. The nucleic acid extraction system provided by the present invention comprises: a reagent chamber, provided with a sample elution chamber and at least two reagent carrying chambers; pistons are slidably provided in the sample elution chamber and the reagent carrying chamber; outlets are respectively provided at one end of the sample elution chamber and the reagent carrying chamber; an injection port is provided on the sample elution chamber; an extraction operation component cooperates with the reagent chamber, provided with a reaction chamber and a nucleic acid outlet, and a fourth microvalve is provided between the reaction chamber and the nucleic acid outlet; a plurality of reagent inlets are provided on the extraction operation component to dock with the outlets of the sample elution chamber and the reagent carrying chamber, and the plurality of reagent inlets are connected through internal channels; at least part of the internal channels is connected with the reaction chamber, and a nucleic acid capture object is provided in the reaction chamber; when in use, the corresponding reagents enter the sample elution chamber, the reagent carrying chamber and the reaction chamber through the sliding and lifting of different pistons, and the nucleic acid is captured by the nucleic acid capture object in the reaction chamber, and discharged to the nucleic acid analysis end through the nucleic acid outlet; the present application adopts the above technical solution, and the air pressure balance and the flow of reagents in the closed system are achieved through the sliding and lifting of different pistons; finally, the extracted nucleic acid is injected into various downstream nucleic acid analysis ends through the nucleic acid outlet. The general automated nucleic acid extraction solution provided by the technical solution of the present application, relying on the full closure, full process integration and automation of nucleic acid extraction, can provide effective support for a variety of nucleic acid analysis terminals.
2.本发明提供的核酸提取系统，还包括：弹性密封垫，设置于所述外壳内的试剂仓的下方，所述样本洗脱腔和试剂承载腔的出口的针头插入所述弹性密封垫内，以密封所述样本洗脱腔和试剂承载腔，使用时，所述试剂仓在所述外壳内通过朝向所述提取操作组件滑动，以使所述针头贯穿所述弹性密封垫；本申请采用上述技术方案，通过在针头插入所述弹性密封垫内，针头的位置确保密封试剂；在使用时，结合弹性密封垫，确保密封效果。2. The nucleic acid extraction system provided by the present invention also includes: an elastic sealing gasket, which is arranged below the reagent chamber in the outer shell, and the needles at the outlets of the sample elution chamber and the reagent carrying chamber are inserted into the elastic sealing gasket to seal the sample elution chamber and the reagent carrying chamber. When in use, the reagent chamber slides toward the extraction operation component in the outer shell so that the needle penetrates the elastic sealing gasket; the present application adopts the above technical solution, and the position of the needle ensures the sealing reagent by inserting the needle into the elastic sealing gasket; when in use, combined with the elastic sealing gasket, the sealing effect is ensured.
3.本发明在所述提取操作组件内还设有废液腔和排气口，所述排气口与废液腔之间设有第二微阀，所述废液腔与反应腔之间设有第三微阀；在所述废液腔内置有吸水材料，所述吸水材料适于吸取废液；本申请采用上述技术方案，通过废液腔吸收废液，防止污染。3. The present invention further provides a waste liquid chamber and an exhaust port in the extraction operation component, a second microvalve is provided between the exhaust port and the waste liquid chamber, and a third microvalve is provided between the waste liquid chamber and the reaction chamber; a water-absorbing material is provided in the waste liquid chamber, and the water-absorbing material is suitable for absorbing waste liquid; the present application adopts the above technical solution to absorb waste liquid through the waste liquid chamber to prevent pollution.
4.本发明所述试剂承载腔包括：裂解腔，所述裂解腔与废液腔通过蛇形防反流流道连通；在所述裂解腔与反应腔之间的内部通道上设有第一微阀；本申请采用上述技术方案，通过蛇形防反流流道，防止液体试剂通过裂解腔向废液腔反流。4. The reagent-carrying chamber described in the present invention includes: a lysis chamber, which is connected to the waste liquid chamber through a serpentine anti-backflow channel; a first microvalve is provided on the internal channel between the lysis chamber and the reaction chamber; the present application adopts the above-mentioned technical solution to prevent the liquid reagent from flowing back to the waste liquid chamber through the lysis chamber through the serpentine anti-backflow channel.
附图说明BRIEF DESCRIPTION OF THE DRAWINGS
为了更清楚地说明本发明具体实施方式或现有技术中的技术方案，下面将对具体实施方式或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图是本发明的一些实施方式，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
图1为本发明实施方式一中提供的核酸提取系统的立体透视结构示意图；FIG1 is a perspective structural diagram of a nucleic acid extraction system provided in Embodiment 1 of the present invention;
图2为本发明实施方式一中提供的核酸提取系统的立体结构示意图；FIG2 is a schematic diagram of the three-dimensional structure of a nucleic acid extraction system provided in Embodiment 1 of the present invention;
图3为本发明实施方式一中提供的核酸提取系统的立体分解结构示意图；FIG3 is a schematic diagram of the three-dimensional exploded structure of the nucleic acid extraction system provided in the first embodiment of the present invention;
图4为本发明实施方式一中提供的流道模块的立体透视结构示意图；FIG4 is a perspective structural diagram of a flow channel module provided in Embodiment 1 of the present invention;
图5为本发明实施方式一中提供的接口结构的立体结构示意图；FIG5 is a schematic diagram of the three-dimensional structure of the interface structure provided in the first embodiment of the present invention;
图6为本发明实施方式一中提供的试剂仓的立体透视结构示意图；FIG6 is a perspective structural diagram of a reagent chamber provided in Embodiment 1 of the present invention;
图7为本发明实施方式一中提供的试剂仓在组装状态下的剖视结构示意图；FIG7 is a schematic cross-sectional view of the reagent compartment in an assembled state provided in Embodiment 1 of the present invention;
图8为本发明实施方式一中提供的试剂仓在使用状态下的剖视结构示意图；FIG8 is a schematic cross-sectional view of the reagent chamber in use according to the first embodiment of the present invention;
图9为本发明实施方式一中提供的第一微阀的剖视结构示意图一；FIG9 is a cross-sectional structural schematic diagram 1 of the first microvalve provided in the first embodiment of the present invention;
图10为本发明实施方式一中提供的第一微阀的剖视结构示意图二；FIG10 is a second schematic cross-sectional structural diagram of the first microvalve provided in the first embodiment of the present invention;
图11为本发明实施方式二中提供的核酸提取系统的立体结构示意图；FIG11 is a schematic diagram of the three-dimensional structure of a nucleic acid extraction system provided in Embodiment 2 of the present invention;
图12为本发明实施方式二中提供的核酸提取系统的立体分解结构示意图；FIG12 is a schematic diagram of a three-dimensional exploded structure of a nucleic acid extraction system provided in Embodiment 2 of the present invention;
图13为本发明实施方式二中提供的流道模块的立体透视结构示意图；FIG13 is a perspective structural diagram of a flow channel module provided in Embodiment 2 of the present invention;
图14为本发明实施方式二中提供的接口结构的立体结构示意图；FIG14 is a schematic diagram of the three-dimensional structure of the interface structure provided in the second embodiment of the present invention;
图15为本发明实施方式二中提供的试剂仓的立体透视结构示意图。FIG. 15 is a schematic diagram of a three-dimensional perspective structure of a reagent chamber provided in the second embodiment of the present invention.
附图标记说明：Description of reference numerals:
1、试剂储存组件；2、提取操作组件；3、推动结构；4、贴纸；5、活塞；6、驱动结构；7、封盖；8、试剂仓；9、针头；10、裂解液腔；11、消化缓冲液腔；12、样本洗脱腔；13、第一清洗液腔；14、乙醇腔；15、第二清洗液腔；16、第一漂洗液腔；17、洗脱液腔；18、第二漂洗液腔；19、气动腔；20、进样口；21、外壳；22、控制通道；23、弹性密封垫；24、弹性密封膜；25、流道模块；26、裂解液入口；27、消化缓冲液入口；28、样本入口；29、第一清洗液入口；30、乙醇入口；31、第二清洗液入口；32、第一漂洗液入口；33、洗脱液入口；34、第二漂洗液入口；35、气体入口；36、反应腔；37、接口结构；38、核酸出口；39、排气口；40、废液腔；41、蛇形防反流流道；42、第一微阀；43、第二微阀；44、第三微阀；45、第四微阀；46、液体流道；47、底板；48、压杆。1. Reagent storage component; 2. Extraction operation component; 3. Pushing structure; 4. Sticker; 5. Piston; 6. Driving structure; 7. Capping; 8. Reagent compartment; 9. Needle; 10. Lysis liquid chamber; 11. Digestion buffer chamber; 12. Sample elution chamber; 13. First cleaning liquid chamber; 14. Ethanol chamber; 15. Second cleaning liquid chamber; 16. First rinsing liquid chamber; 17. Elution liquid chamber; 18. Second rinsing liquid chamber; 19. Pneumatic chamber; 20. Inlet; 21. Housing; 22. Control channel; 23. Elastic sealing pad; 24. Elastic sealing membrane; 25. Flow channel module; 2 6. Lysis solution inlet; 27. Digestion buffer inlet; 28. Sample inlet; 29. First cleaning solution inlet; 30. Ethanol inlet; 31. Second cleaning solution inlet; 32. First rinse solution inlet; 33. Eluent inlet; 34. Second rinse solution inlet; 35. Gas inlet; 36. Reaction chamber; 37. Interface structure; 38. Nucleic acid outlet; 39. Exhaust port; 40. Waste liquid chamber; 41. Serpentine anti-backflow channel; 42. First microvalve; 43. Second microvalve; 44. Third microvalve; 45. Fourth microvalve; 46. Liquid flow channel; 47. Bottom plate; 48. Pressure rod.
具体实施方式Detailed ways
下面将结合附图对本发明的技术方案进行清楚、完整地描述，显然，所描述的实施例是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
在本发明的描述中，需要说明的是，术语“中心”、“上”、“下”、“左”、“右”、“竖直”、“水平”、“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本发明和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。此外，术语“第一”、“第二”、“第三”仅用于描述目的，而不能理解为指示或暗示相对重要性。In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.
在本发明的描述中，需要说明的是，除非另有明确的规定和限定，术语“安装”、“相连”、“连接”应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或一体地连接；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通。对于本领域的普通技术人员而言，可以具体情况理解上述术语在本发明中的具体含义。In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
此外，下面所描述的本发明不同实施方式中所涉及的技术特征只要彼此之间未构成冲突就可以相互结合。In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.
实施方式一Implementation Method 1
如图1至图10所示的核酸提取系统的一种具体实施方式，包括：试剂仓8、外壳21、驱动结构6、弹性密封垫23和提取操作组件2。所述试剂仓8、驱动结构6、弹性密封垫23和外壳21共同构成试剂储存组件1。所述试剂储存组件1用于实现试剂的独立存储、有效密封和快捷使用。所述试剂可以是液体试剂。所述提取操作组件2为液体试剂提供流体通道和反应腔室，同时还能够为固体试剂提供独立的封装空间，实现液体试剂的输运和核酸提取流程的操作。A specific embodiment of the nucleic acid extraction system as shown in Figures 1 to 10 includes: a reagent compartment 8, a housing 21, a drive structure 6, an elastic sealing pad 23 and an extraction operation component 2. The reagent compartment 8, the drive structure 6, the elastic sealing pad 23 and the housing 21 together constitute a reagent storage component 1. The reagent storage component 1 is used to achieve independent storage, effective sealing and quick use of reagents. The reagent can be a liquid reagent. The extraction operation component 2 provides a fluid channel and a reaction chamber for the liquid reagent, and can also provide an independent packaging space for the solid reagent, so as to realize the transportation of the liquid reagent and the operation of the nucleic acid extraction process.
可替换的实施方式，在上述核酸提取系统中，外壳21、驱动结构6和弹性密封垫23可以去除。即所述核酸提取系统仅包括：试剂仓8和提取操作组件2所述试剂仓8设有一个样本洗脱腔12和至少两个试剂承载腔；在所述样本洗脱腔12和试剂承载腔内均滑动设有活塞5；在所述样本洗脱腔12和试剂承载腔的一端分别具有出口；在所述样本洗脱腔12上设有进样口20。所述提取操作组件2与所述试剂仓8配合，且所述提取操作组件2设有反应腔36和核酸出口38，所述反应腔36与核酸出口38之间设有第四微阀45；在所述提取操作组件2上设有与所述样本洗脱腔12和试剂承载腔的出口对接的若干试剂入口，若干所述试剂入口之间通过内部通道连通；至少部分所述内部通道与所述反应腔36连通，所述反应腔36内设有核酸捕获物；在使用时，通过不同活塞5的滑动升降，使得相应的试剂进入样本洗脱腔12、试剂承载腔和反应腔36内，在所述反应腔36内通过核酸捕获物捕获核酸，并通过核酸出口38排出至核酸分析端。本实施方式通过不同活塞5的滑动升降实现封闭系统内的气压平衡和试剂的流动；最后，将提取后的核酸通过核酸出口38注入下游各种核酸分析端。本申请技术方案提供的通用型的自动化核酸提取方案，依托核酸提取的全封闭化、全流程一体化和自动化，可为多样化的核酸分析端提供有效支持。In an alternative embodiment, in the above nucleic acid extraction system, the housing 21, the driving structure 6 and the elastic sealing pad 23 can be removed. That is, the nucleic acid extraction system only includes: a reagent chamber 8 and an extraction operation component 2. The reagent chamber 8 is provided with a sample elution chamber 12 and at least two reagent carrying chambers; a piston 5 is slidably provided in the sample elution chamber 12 and the reagent carrying chamber; an outlet is provided at one end of the sample elution chamber 12 and the reagent carrying chamber respectively; and an injection port 20 is provided on the sample elution chamber 12. The extraction operation component 2 cooperates with the reagent compartment 8, and the extraction operation component 2 is provided with a reaction chamber 36 and a nucleic acid outlet 38, and a fourth microvalve 45 is provided between the reaction chamber 36 and the nucleic acid outlet 38; the extraction operation component 2 is provided with a plurality of reagent inlets docked with the outlets of the sample elution chamber 12 and the reagent carrying chamber, and the plurality of reagent inlets are connected through internal channels; at least part of the internal channels are connected with the reaction chamber 36, and a nucleic acid capture object is provided in the reaction chamber 36; when in use, the corresponding reagents enter the sample elution chamber 12, the reagent carrying chamber and the reaction chamber 36 through the sliding and lifting of different pistons 5, and the nucleic acid is captured by the nucleic acid capture object in the reaction chamber 36, and discharged to the nucleic acid analysis end through the nucleic acid outlet 38. In this embodiment, the air pressure balance and the flow of the reagent in the closed system are achieved by the sliding and lifting of different pistons 5; finally, the extracted nucleic acid is injected into various downstream nucleic acid analysis ends through the nucleic acid outlet 38. The universal automated nucleic acid extraction scheme provided by the technical solution of the present application can provide effective support for diversified nucleic acid analysis ends by relying on the full closure, full process integration and automation of nucleic acid extraction.
如图1至图3、图5和图6所示，所述试剂仓8设有一个样本洗脱腔12和至少两个试剂承载腔；所述样本洗脱腔12和试剂承载腔的体积不小于50μL。样本洗脱腔12和试剂承载腔的体积可以相同，也可以不同。样本洗脱腔12和试剂承载腔的横截面可以为圆形、椭圆形、正方形、长方形或多边形，样本洗脱腔12和试剂承载腔的横截面可以相同，也可以不同。所述样本洗脱腔12和试剂承载腔的底部可以为平底、锥底或球形底，样本洗脱腔12和试剂承载腔的底部可以相同，也可以不同。如图6所示，所述样本洗脱腔12和试剂承载腔的底部均为锥底，所述样本洗脱腔12和试剂承载腔的横截面均为圆形。在所述样本洗脱腔12和试剂承载腔内均滑动设有活塞5；活塞5严密贴合样本洗脱腔12和试剂承载腔，从而在活塞5上下移动的过程中可以保持密封。在所述样本洗脱腔12和试剂承载腔的出口连接有针头9；所述试剂仓8在所述外壳21内通过朝向所述提取操作组件2滑动，以使所述样本洗脱腔12和试剂承载腔的出口与所述提取操作组件2的试剂入口连通。针头9的内端穿透所述样本洗脱腔12和试剂承载腔的底部。所述针头9的外端可以是尖头或平头。如图6所示，所述针头9的外端是尖头。所述针头9的材质可以是不锈钢或塑料。在所述样本洗脱腔12上设有进样口20；具体的，如图6所示，进样口20设置于所述样本洗脱腔12的侧面；如图3所示，在所述进样口20螺纹连接有封盖7，实现样本进样以及全体系的密封。所述样本洗脱腔12适于存储试剂和通过进样口20引入液体样本或拭子；所述液体样本可以为唾液、处理后的痰液、血液或拭子涮洗液等。所述试剂承载腔适于存储试剂。样本洗脱腔12和试剂承载腔内的试剂种类可以相同，也可以不同。所述试剂可以是液体，也可以是干粉。所述试剂仓8与所述外壳21连接，且所述外壳21压附在所述弹性密封膜24上。所述驱动结构6与所述活塞5连接，所述驱动结构6适于驱动活塞5上下滑动；所述驱动结构6安装于外壳21内。如图7和图8所示，所述弹性密封垫23设置于所述外壳21内的试剂仓8的下方，所述样本洗脱腔12和试剂承载腔的出口的针头9插入所述弹性密封垫23内，以密封所述样本洗脱腔12和试剂承载腔，使用时，所述试剂仓8在所述外壳21内通过朝向所述提取操作组件2滑动，以使所述针头9贯穿所述弹性密封垫23。即所述试剂仓8具有所述针头9的外端位于所述弹性密封垫23内，以密封所述样本洗脱腔12和试剂承载腔的组装状态，以及具有所述针头9的外端刺破贯穿所述弹性密封垫23的使用状态。本实施方式通过在针头插入所述弹性密封垫内，针头的位置确保密封试剂；在使用时，结合弹性密封垫，确保密封效果。在使用状态下，试剂仓8的下表面与外壳21的下表面仍存在一定距离，实现刺破位置控制，防止针头9的过度移位。所述弹性密封垫23的材质可以是硅胶、橡胶或其它具有高弹性的聚合物材料。所述核酸提取系统适于放置于便携式仪器内，在所述便携式仪器内对应试剂仓8的位置设有推动结构3，所述推动结构3适于推动所述试剂仓8由组装状态变为使用状态。所述提取操作组件2设有连通的反应腔36和核酸出口38；在所述提取操作组件2上设有与所述针头9对应的试剂入口；所述试剂入口与反应腔36连通；所述反应腔36与核酸出口38连通；在所述使用状态下，所述针头9与对应的试剂入口连通；所述反应腔36适于存储核酸捕获物。所述核酸提取系统适于在所述使用状态下，通过不同活塞5的滑动升降，使得相应的试剂进入样本洗脱腔12和反应腔36内，以通过核酸捕获物捕获核酸，并通过核酸出口38排出至核酸分析端。As shown in Figures 1 to 3, Figure 5 and Figure 6, the reagent compartment 8 is provided with a sample elution chamber 12 and at least two reagent carrying chambers; the volume of the sample elution chamber 12 and the reagent carrying chamber is not less than 50 μL. The volume of the sample elution chamber 12 and the reagent carrying chamber can be the same or different. The cross-section of the sample elution chamber 12 and the reagent carrying chamber can be circular, elliptical, square, rectangular or polygonal, and the cross-section of the sample elution chamber 12 and the reagent carrying chamber can be the same or different. The bottom of the sample elution chamber 12 and the reagent carrying chamber can be flat, conical or spherical, and the bottom of the sample elution chamber 12 and the reagent carrying chamber can be the same or different. As shown in Figure 6, the bottom of the sample elution chamber 12 and the reagent carrying chamber are both conical bottoms, and the cross-section of the sample elution chamber 12 and the reagent carrying chamber are both circular. A piston 5 is slidably provided in the sample elution chamber 12 and the reagent carrying chamber; the piston 5 fits the sample elution chamber 12 and the reagent carrying chamber tightly, so that the seal can be maintained during the up and down movement of the piston 5. The outlets of the sample elution chamber 12 and the reagent carrying chamber are connected with a needle 9; the reagent chamber 8 slides toward the extraction operation component 2 in the housing 21 so that the outlets of the sample elution chamber 12 and the reagent carrying chamber are connected with the reagent inlet of the extraction operation component 2. The inner end of the needle 9 penetrates the bottom of the sample elution chamber 12 and the reagent carrying chamber. The outer end of the needle 9 can be a pointed head or a flat head. As shown in FIG6 , the outer end of the needle 9 is a pointed head. The material of the needle 9 can be stainless steel or plastic. An injection port 20 is provided on the sample elution chamber 12; specifically, as shown in FIG6 , the injection port 20 is arranged on the side of the sample elution chamber 12; as shown in FIG3 , a cover 7 is threadedly connected to the injection port 20 to achieve sample injection and sealing of the entire system. The sample elution chamber 12 is suitable for storing reagents and introducing liquid samples or swabs through the injection port 20; the liquid sample can be saliva, processed sputum, blood or swab washing liquid, etc. The reagent carrying chamber is suitable for storing reagents. The types of reagents in the sample elution chamber 12 and the reagent carrying chamber can be the same or different. The reagent can be a liquid or a dry powder. The reagent chamber 8 is connected to the housing 21, and the housing 21 is pressed against the elastic sealing film 24. The driving structure 6 is connected to the piston 5, and the driving structure 6 is suitable for driving the piston 5 to slide up and down; the driving structure 6 is installed in the housing 21. As shown in Figures 7 and 8, the elastic sealing pad 23 is arranged below the reagent chamber 8 in the housing 21, and the needle 9 at the outlet of the sample elution chamber 12 and the reagent carrying chamber is inserted into the elastic sealing pad 23 to seal the sample elution chamber 12 and the reagent carrying chamber. When in use, the reagent chamber 8 slides toward the extraction operation component 2 in the housing 21 so that the needle 9 penetrates the elastic sealing pad 23. That is, the reagent chamber 8 has an assembly state in which the outer end of the needle 9 is located in the elastic sealing pad 23 to seal the sample elution chamber 12 and the reagent carrying chamber, and a use state in which the outer end of the needle 9 pierces and penetrates the elastic sealing pad 23. In this embodiment, the needle is inserted into the elastic sealing pad, and the position of the needle ensures the sealing reagent; when in use, the elastic sealing pad is combined to ensure the sealing effect. In the use state, there is still a certain distance between the lower surface of the reagent compartment 8 and the lower surface of the housing 21, so as to achieve the puncture position control and prevent the excessive displacement of the needle 9. The material of the elastic sealing pad 23 can be silicone, rubber or other polymer materials with high elasticity. The nucleic acid extraction system is suitable for being placed in a portable instrument, and a pushing structure 3 is provided at the position corresponding to the reagent compartment 8 in the portable instrument, and the pushing structure 3 is suitable for pushing the reagent compartment 8 from the assembly state to the use state. The extraction operation component 2 is provided with a connected reaction chamber 36 and a nucleic acid outlet 38; a reagent inlet corresponding to the needle 9 is provided on the extraction operation component 2; the reagent inlet is connected to the reaction chamber 36; the reaction chamber 36 is connected to the nucleic acid outlet 38; in the use state, the needle 9 is connected to the corresponding reagent inlet; the reaction chamber 36 is suitable for storing nucleic acid capture objects. The nucleic acid extraction system is suitable for, in the use state, sliding and lifting different pistons 5 so that the corresponding reagents enter the sample elution chamber 12 and the reaction chamber 36, so as to capture nucleic acids through the nucleic acid capture object and discharge them to the nucleic acid analysis end through the nucleic acid outlet 38.
如图3、图4和图5所示，所述提取操作组件2包括：依次贴合设置的弹性密封膜24、流道模块25和底板47。在所述底板47内设有反应腔36，在所述流道模块25内设有内部通道。在所述流道模块25上设有与所述针头9对应的试剂入口；所述提取操作组件2还设有废液腔40和排气口39；在所述排气口39与废液腔40之间设有第二微阀43；在所述废液腔40与反应腔36之间设有第三微阀44；在所述废液腔40内置有吸水材料，所述吸水材料适于吸取废液。所述吸水材料可以是多孔吸水材料。本实施方式通过废液腔吸收废液，防止污染。在所述流道模块25上设有排气口39。如图4和图5所示，所述核酸出口38和排气口39可以共同设置于接口结构37内，所述接口结构37可以与下游的检测端插接；或者所述接口结构37设有内螺纹，可以与下游的检测端螺纹连接，完成核酸的自动化提取及检测过程。在所述反应腔36与核酸出口38之间设有第四微阀45。As shown in Figures 3, 4 and 5, the extraction operation component 2 includes: an elastic sealing membrane 24, a flow channel module 25 and a bottom plate 47 that are sequentially arranged. A reaction chamber 36 is provided in the bottom plate 47, and an internal channel is provided in the flow channel module 25. A reagent inlet corresponding to the needle 9 is provided on the flow channel module 25; the extraction operation component 2 is also provided with a waste liquid chamber 40 and an exhaust port 39; a second microvalve 43 is provided between the exhaust port 39 and the waste liquid chamber 40; a third microvalve 44 is provided between the waste liquid chamber 40 and the reaction chamber 36; a water-absorbing material is built into the waste liquid chamber 40, and the water-absorbing material is suitable for absorbing waste liquid. The water-absorbing material can be a porous water-absorbing material. This embodiment absorbs waste liquid through the waste liquid chamber to prevent contamination. An exhaust port 39 is provided on the flow channel module 25. As shown in Figures 4 and 5, the nucleic acid outlet 38 and the exhaust port 39 can be arranged together in the interface structure 37, and the interface structure 37 can be plugged with the downstream detection end; or the interface structure 37 is provided with an internal thread, which can be threadedly connected with the downstream detection end to complete the automatic extraction and detection process of nucleic acid. A fourth microvalve 45 is provided between the reaction chamber 36 and the nucleic acid outlet 38.
可替换的实施方式，在上述核酸提取系统中，在所述核酸提取系统仅包括：试剂仓8和提取操作组件2的基础上，在所述提取操作组件2内还设有废液腔40和排气口39，所述排气口39与废液腔40之间设有第二微阀43，所述废液腔40与反应腔36之间设有第三微阀44；在所述废液腔40内置有吸水材料，所述吸水材料适于吸取废液。通过废液腔40吸收废液，防止污染，通过第二微阀43控制排气口39与废液腔40是否连通，当连通时，排气口39进行排气。通过第三微阀44控制废液腔40与反应腔36是否连通。In an alternative embodiment, in the above-mentioned nucleic acid extraction system, the nucleic acid extraction system only includes: on the basis of the reagent compartment 8 and the extraction operation component 2, a waste liquid chamber 40 and an exhaust port 39 are further provided in the extraction operation component 2, a second microvalve 43 is provided between the exhaust port 39 and the waste liquid chamber 40, and a third microvalve 44 is provided between the waste liquid chamber 40 and the reaction chamber 36; a water-absorbing material is built into the waste liquid chamber 40, and the water-absorbing material is suitable for absorbing waste liquid. The waste liquid is absorbed by the waste liquid chamber 40 to prevent contamination, and the exhaust port 39 is controlled by the second microvalve 43 to be connected with the waste liquid chamber 40. When connected, the exhaust port 39 is exhausted. Whether the waste liquid chamber 40 is connected with the reaction chamber 36 is controlled by the third microvalve 44.
可替换的实施方式，在上述核酸提取系统中，所述提取操作组件2仅包括：依次贴合设置的弹性密封膜24、流道模块25和底板47；在所述底板47内设有反应腔36，在所述流道模块25内设有内部通道。通过不同活塞5的滑动升降实现封闭系统内的气压平衡和试剂的流动；试剂在流道模块25的内部通道中流动，最终流入反应腔36内，捕获核酸，将提取后的核酸通过核酸出口38注入下游各种核酸分析端。In an alternative embodiment, in the above nucleic acid extraction system, the extraction operation component 2 only includes: an elastic sealing membrane 24, a flow channel module 25 and a bottom plate 47 which are sequentially arranged; a reaction chamber 36 is provided in the bottom plate 47, and an internal channel is provided in the flow channel module 25. The air pressure balance and the flow of reagents in the closed system are achieved by sliding and lifting different pistons 5; the reagents flow in the internal channel of the flow channel module 25, and finally flow into the reaction chamber 36 to capture nucleic acids, and the extracted nucleic acids are injected into various downstream nucleic acid analysis terminals through the nucleic acid outlet 38.
可替换的实施方式，在上述核酸提取系统中，所述核酸提取系统在包括：试剂仓8和提取操作组件2的基础上，还仅包括：外壳21，所述试剂仓8与所述外壳21连接，且所述外壳21压附在所述弹性密封膜24上，以固定所述弹性密封膜24。In an alternative embodiment, in the above-mentioned nucleic acid extraction system, the nucleic acid extraction system, in addition to including: a reagent chamber 8 and an extraction operation component 2, further only includes: a shell 21, the reagent chamber 8 is connected to the shell 21, and the shell 21 is pressed onto the elastic sealing membrane 24 to fix the elastic sealing membrane 24.
可替换的实施方式，在上述核酸提取系统中，所述核酸提取系统在包括：试剂仓8、提取操作组件2和外壳21的基础上，还仅包括：第四微阀45。所述反应腔36与核酸出口38之间设有第四微阀45。在所述外壳21上设有沿竖直方向的控制通道22；所述第四微阀45包括：控制腔室和压杆48；所述控制腔室设置于所述提取操作组件2内；所述控制通道22与所述控制腔室相对应；所述压杆48适于在所述控制通道22中滑动上升或下降，以分别放松或推压所述弹性密封膜24至控制腔室，进而分别连通或断开所连接的管路。通过第四微阀45控制反应腔36内的核酸能否从核酸出口38排出。In an alternative embodiment, in the above-mentioned nucleic acid extraction system, the nucleic acid extraction system, on the basis of including: the reagent compartment 8, the extraction operation component 2 and the housing 21, further includes: the fourth microvalve 45. A fourth microvalve 45 is provided between the reaction chamber 36 and the nucleic acid outlet 38. A control channel 22 is provided in the vertical direction on the housing 21; the fourth microvalve 45 includes: a control chamber and a pressure rod 48; the control chamber is arranged in the extraction operation component 2; the control channel 22 corresponds to the control chamber; the pressure rod 48 is suitable for sliding up or down in the control channel 22 to respectively relax or push the elastic sealing membrane 24 to the control chamber, thereby respectively connecting or disconnecting the connected pipeline. Whether the nucleic acid in the reaction chamber 36 can be discharged from the nucleic acid outlet 38 is controlled by the fourth microvalve 45.
进一步的，如图3所示，在所述外壳21上设有贴纸4；具体的，贴纸4设置于外壳21的顶部，所述贴纸4适于封装，且在贴纸4上设有标识核酸提取系统是否有效的标记。Furthermore, as shown in FIG. 3 , a sticker 4 is provided on the housing 21 ; specifically, the sticker 4 is provided on the top of the housing 21 , the sticker 4 is suitable for packaging, and a mark indicating whether the nucleic acid extraction system is effective is provided on the sticker 4 .
在搭载标准磁珠提取技术时，所述核酸捕获物为结合试剂和磁珠，在所述反应腔36的底部设有电磁铁。如图3至图6所示，在所述反应腔36的底部设有电磁铁；所述试剂承载腔包括：裂解腔、清洗腔、洗脱液腔17和气动腔19。所述裂解腔为裂解液腔10，所述清洗腔包括第一清洗液腔13和第二清洗液腔15。所述裂解腔与废液腔40通过蛇形防反流流道41连通；本实施方式通过蛇形防反流流道41，防止液体试剂通过裂解腔向废液腔反流。在所述裂解腔与反应腔36之间的内部通道上设有第一微阀42。所述裂解液腔10、样本洗脱腔12、第一清洗液腔13、第二清洗液腔15和气动腔19的直径尺寸一致，均为洗脱液腔17直径的二倍。在所述裂解液腔10内存储有裂解液，在所述第一清洗液腔13内存储有第一清洗液，在所述第二清洗液腔15内存储有第二清洗液，在所述洗脱液腔17内存储有洗脱液，在所述气动腔19内存储有空气；空气可作为驱动力驱动液体流动；具体在使用时，可以通过下压所述气动腔19的活塞5，用于驱动所述反应腔36内的核酸流至核酸出口38。在所述反应腔36内存储有结合试剂和磁珠；具体的，所述结合试剂为干化的结合试剂，所述磁珠为冻干磁珠。所述裂解液腔10、样本洗脱腔12、第一清洗液腔13、第二清洗液腔15、洗脱液腔17和气动腔19对应的试剂入口分别为：裂解液入口26、样本入口28、第一清洗液入口29、第二清洗液入口31、洗脱液入口33和气体入口35；所述裂解液入口26通过第一微阀42与样本入口28连通；所述裂解液入口26与废液腔40通过蛇形防反流流道41连通；所述样本入口28、第一清洗液入口29、第二清洗液入口31、洗脱液入口33、气体入口35均与反应腔36连通；具体的，所述样本入口28、第一清洗液入口29、第二清洗液入口31、洗脱液入口33、气体入口35均通过液体流道46与反应腔36连通。When the standard magnetic bead extraction technology is used, the nucleic acid capture object is a binding reagent and magnetic beads, and an electromagnet is provided at the bottom of the reaction chamber 36. As shown in Figures 3 to 6, an electromagnet is provided at the bottom of the reaction chamber 36; the reagent carrying chamber includes: a lysis chamber, a cleaning chamber, an elution chamber 17 and a pneumatic chamber 19. The lysis chamber is a lysis chamber 10, and the cleaning chamber includes a first cleaning chamber 13 and a second cleaning chamber 15. The lysis chamber is connected to the waste liquid chamber 40 through a serpentine anti-backflow channel 41; this embodiment prevents the liquid reagent from flowing back through the lysis chamber to the waste liquid chamber through the serpentine anti-backflow channel 41. A first microvalve 42 is provided on the internal channel between the lysis chamber and the reaction chamber 36. The diameters of the lysis chamber 10, the sample elution chamber 12, the first cleaning chamber 13, the second cleaning chamber 15 and the pneumatic chamber 19 are consistent, all of which are twice the diameter of the elution chamber 17. The lysate is stored in the lysate chamber 10, the first cleaning liquid is stored in the first cleaning liquid chamber 13, the second cleaning liquid is stored in the second cleaning liquid chamber 15, the elution liquid is stored in the elution chamber 17, and the air is stored in the pneumatic chamber 19; the air can be used as a driving force to drive the liquid to flow; specifically, when in use, the piston 5 of the pneumatic chamber 19 can be pressed down to drive the nucleic acid in the reaction chamber 36 to flow to the nucleic acid outlet 38. The reaction chamber 36 stores binding reagents and magnetic beads; specifically, the binding reagents are dried binding reagents, and the magnetic beads are freeze-dried magnetic beads. The reagent inlets corresponding to the lysate chamber 10, the sample elution chamber 12, the first cleaning liquid chamber 13, the second cleaning liquid chamber 15, the eluent chamber 17 and the pneumatic chamber 19 are respectively: a lysate inlet 26, a sample inlet 28, a first cleaning liquid inlet 29, a second cleaning liquid inlet 31, an eluent inlet 33 and a gas inlet 35; the lysate inlet 26 is connected to the sample inlet 28 through a first microvalve 42; the lysate inlet 26 is connected to the waste liquid chamber 40 through a serpentine anti-backflow channel 41; the sample inlet 28, the first cleaning liquid inlet 29, the second cleaning liquid inlet 31, the eluent inlet 33 and the gas inlet 35 are all connected to the reaction chamber 36; specifically, the sample inlet 28, the first cleaning liquid inlet 29, the second cleaning liquid inlet 31, the eluent inlet 33 and the gas inlet 35 are all connected to the reaction chamber 36 through a liquid channel 46.
可替换的实施方式，在上述核酸提取系统中，所述核酸提取系统在包括：试剂仓8、提取操作组件2、外壳21的基础上，所述试剂仓8滑动连接于所述外壳21内；在所述样本洗脱腔12和试剂承载腔的出口连接有针头9，所述试剂仓8在所述外壳21内通过朝向所述提取操作组件2滑动，以使所述样本洗脱腔12和试剂承载腔的出口与所述提取操作组件2的试剂入口连通。通过试剂仓8的滑动，方便连通样本洗脱腔12和试剂承载腔的出口与所述提取操作组件2的试剂入口。In an alternative embodiment, in the above nucleic acid extraction system, the nucleic acid extraction system includes: a reagent chamber 8, an extraction operation component 2, and a housing 21, wherein the reagent chamber 8 is slidably connected to the housing 21; a needle 9 is connected to the outlets of the sample elution chamber 12 and the reagent carrying chamber, and the reagent chamber 8 slides toward the extraction operation component 2 in the housing 21 to connect the outlets of the sample elution chamber 12 and the reagent carrying chamber with the reagent inlet of the extraction operation component 2. The sliding of the reagent chamber 8 facilitates the connection between the outlets of the sample elution chamber 12 and the reagent carrying chamber and the reagent inlet of the extraction operation component 2.
可替换的实施方式，在上述核酸提取系统中，所述核酸提取系统在包括：试剂仓8、提取操作组件2、外壳21的基础上，还仅包括：弹性密封垫23，所述弹性密封垫23设置于所述外壳21内的试剂仓8的下方，所述样本洗脱腔12和试剂承载腔的出口的针头9插入所述弹性密封垫23内，以密封所述样本洗脱腔12和试剂承载腔，使用时，所述试剂仓8在所述外壳21内通过朝向所述提取操作组件2滑动，以使所述针头9贯穿所述弹性密封垫23。通过针头9的动作配合弹性密封垫23，实现试剂在密封空间内流动。In an alternative embodiment, in the above nucleic acid extraction system, the nucleic acid extraction system, in addition to comprising: a reagent compartment 8, an extraction operation component 2, and a housing 21, further comprises: an elastic sealing pad 23, the elastic sealing pad 23 being arranged below the reagent compartment 8 in the housing 21, the needle 9 at the outlet of the sample elution chamber 12 and the reagent carrying chamber being inserted into the elastic sealing pad 23 to seal the sample elution chamber 12 and the reagent carrying chamber, and when in use, the reagent compartment 8 slides toward the extraction operation component 2 in the housing 21 so that the needle 9 penetrates the elastic sealing pad 23. The action of the needle 9 cooperates with the elastic sealing pad 23 to achieve the flow of reagents in the sealed space.
可替换的实施方式，在上述核酸提取系统中，在所述核酸提取系统仅包括：试剂仓8和提取操作组件2的基础上，所述试剂承载腔包括：裂解腔，所述裂解腔与废液腔40通过蛇形防反流流道41连通；在所述裂解腔与反应腔36之间的内部通道上设有第一微阀42。通过蛇形防反流流道41防止裂解腔的试剂流向废液腔40；通过第一微阀42控制裂解腔与反应腔36是否连通。In an alternative embodiment, in the above nucleic acid extraction system, on the basis that the nucleic acid extraction system only includes: the reagent compartment 8 and the extraction operation assembly 2, the reagent carrying chamber includes: a lysis chamber, the lysis chamber is connected to the waste liquid chamber 40 through a serpentine anti-backflow channel 41; a first microvalve 42 is provided on the internal channel between the lysis chamber and the reaction chamber 36. The serpentine anti-backflow channel 41 prevents the reagent in the lysis chamber from flowing to the waste liquid chamber 40; the first microvalve 42 controls whether the lysis chamber is connected to the reaction chamber 36.
可替换的实施方式，在上述核酸提取系统中，在所述核酸提取系统仅包括：试剂仓8和提取操作组件2的基础上，所述试剂承载腔包括：裂解腔、清洗腔和洗脱液腔17，所述清洗腔包括第一清洗液腔13和第二清洗液腔15；在所述裂解腔内存储有裂解液，在所述第一清洗液腔13内存储有第一清洗液，在所述第二清洗液腔15内存储有第二清洗液，所述洗脱液腔17内存储有洗脱液。通过裂解液对进样口20的进样进行裂解，裂解后的产物进入反应腔36，经过第一清洗液和第二清洗液的两次清洗，然后，洗脱液进入反应腔36，洗脱出核酸，核酸再排出。In an alternative embodiment, in the above nucleic acid extraction system, on the basis that the nucleic acid extraction system only includes: the reagent compartment 8 and the extraction operation component 2, the reagent carrying chamber includes: a lysis chamber, a cleaning chamber and an eluent chamber 17, and the cleaning chamber includes a first cleaning chamber 13 and a second cleaning chamber 15; a lysis liquid is stored in the lysis chamber, a first cleaning liquid is stored in the first cleaning chamber 13, a second cleaning liquid is stored in the second cleaning chamber 15, and an eluent is stored in the eluent chamber 17. The sample injected into the injection port 20 is lysed by the lysis liquid, and the lysed product enters the reaction chamber 36, and is cleaned twice by the first cleaning liquid and the second cleaning liquid. Then, the eluent enters the reaction chamber 36 to elute the nucleic acid, and the nucleic acid is discharged.
可替换的实施方式，在上述核酸提取系统中，在所述核酸提取系统仅包括：试剂仓8和提取操作组件2的基础上，所述核酸捕获物为结合试剂和磁珠，在所述反应腔36的底部设有电磁铁。通过反应腔36内的结合试剂和磁珠，将核酸捕获至磁珠上，通过电磁铁通电，固定所述磁珠。In an alternative embodiment, in the above nucleic acid extraction system, the nucleic acid extraction system only includes: the reagent compartment 8 and the extraction operation component 2, the nucleic acid capture object is a binding reagent and magnetic beads, and an electromagnet is provided at the bottom of the reaction chamber 36. The nucleic acid is captured on the magnetic beads by the binding reagent and magnetic beads in the reaction chamber 36, and the magnetic beads are fixed by energizing the electromagnet.
可替换的实施方式，在上述核酸提取系统中，在所述核酸提取系统仅包括：试剂仓8和提取操作组件2和所述核酸捕获物为结合试剂和磁珠的基础上，所述试剂承载腔包括：气动腔19；在所述气动腔19内存储有空气；使用时，通过下压所述气动腔19的活塞5，用于驱动所述反应腔36内的核酸流至核酸出口38。通过气动腔19内的活塞5，推动空气，进而驱动核酸排出。In an alternative embodiment, in the above nucleic acid extraction system, the nucleic acid extraction system only includes: the reagent compartment 8 and the extraction operation component 2 and the nucleic acid capture object is a combination of reagents and magnetic beads, and the reagent carrying chamber includes: a pneumatic chamber 19; air is stored in the pneumatic chamber 19; when in use, the piston 5 of the pneumatic chamber 19 is pressed down to drive the nucleic acid in the reaction chamber 36 to flow to the nucleic acid outlet 38. The piston 5 in the pneumatic chamber 19 pushes the air, thereby driving the nucleic acid to be discharged.
如图3和图4所示，在所述提取操作组件2与外壳21之间设有弹性密封膜24；弹性密封膜24具备一定的形变能力。弹性密封膜24与流道模块25的上表面贴合，底板47与流道模块25的下表面贴合。所述第一微阀42、第二微阀43、第三微阀44和第四微阀45的结构均相同。在使用状态时，针头9刺破所述弹性密封膜24。As shown in Fig. 3 and Fig. 4, an elastic sealing film 24 is provided between the extraction operation assembly 2 and the housing 21; the elastic sealing film 24 has a certain deformation capability. The elastic sealing film 24 is in contact with the upper surface of the flow channel module 25, and the bottom plate 47 is in contact with the lower surface of the flow channel module 25. The structures of the first microvalve 42, the second microvalve 43, the third microvalve 44 and the fourth microvalve 45 are the same. When in use, the needle 9 pierces the elastic sealing film 24.
如图3、图9和图10所示，在所述外壳21上设有沿竖直方向的控制通道22。所述第一微阀42包括：控制腔室和压杆48。所述控制腔室设置于所述提取操作组件2内；所述控制通道22与所述控制腔室相对应。具体的，外壳21的上部用于承载试剂仓8，所述试剂仓8滑动连接于所述外壳21内。外壳21的下部用于衔接弹性密封垫23，外壳21的侧面具有控制通道22。As shown in Fig. 3, Fig. 9 and Fig. 10, a control channel 22 is provided in the vertical direction on the shell 21. The first microvalve 42 includes: a control chamber and a pressure rod 48. The control chamber is arranged in the extraction operation component 2; the control channel 22 corresponds to the control chamber. Specifically, the upper part of the shell 21 is used to carry the reagent chamber 8, and the reagent chamber 8 is slidably connected in the shell 21. The lower part of the shell 21 is used to connect the elastic sealing pad 23, and the side of the shell 21 has a control channel 22.
所述压杆48适于在控制通道22中滑动上升或下降，以分别放松或推压所述弹性密封膜24至控制腔室，进而分别连通或断开所连接的管路，以控制液体的流通或阻断。The pressure rod 48 is suitable for sliding up or down in the control channel 22 to loosen or push the elastic sealing membrane 24 to the control chamber, thereby connecting or disconnecting the connected pipeline to control the circulation or blocking of the liquid.
本申请所述核酸提取系统的工作过程简述如下：在打开第一微阀42，关闭第二微阀43、第三微阀44和第四微阀45时，下压对应裂解液腔10的活塞5，同时上提对应样本洗脱腔12的活塞5，驱动裂解液腔10中的裂解液流入样本洗脱腔12内，对液体样本或拭子进行裂解洗脱，获得样本裂解产物；在裂解洗脱完成后，关闭第一微阀42，打开第三微阀44，下压对应样本洗脱腔12的活塞5，同时上提对应裂解液腔10的活塞5，驱动样本裂解产物流入反应腔36内，利用磁珠和结合试剂捕获样本裂解产物内的核酸；核酸捕获完成后，接通电磁铁，将磁珠吸附在反应腔36的底部；继续下压对应样本洗脱腔12的活塞5，同时上提对应裂解液腔10的活塞5，驱动反应腔36内的废液流入废液腔40内。需要说明的是，样本洗脱腔12内的活塞5具有一定的初始高度，经过进样口20的开盖操作后，样本洗脱腔12内存在一定的气体，该气体可作为后续操作中液体流动的驱动力。断开电磁铁，下压对应第一清洗液腔13的活塞5，同时上提对应裂解液腔10的活塞5，驱动第一清洗液流入反应腔36，以清洗磁珠上的杂质；接通电磁铁，将磁珠吸附在反应腔36的底部，继续下压对应样本洗脱腔12的活塞5，同时上提对应裂解液腔10的活塞5，驱动反应腔36内的废液流入废液腔40内；断开电磁铁，下压对应第二清洗液腔15的活塞5，同时上提对应裂解液腔10的活塞5，驱动第二清洗液流入反应腔36，以清洗磁珠上的杂质；接通电磁铁，将磁珠吸附在反应腔36的底部；继续下压对应样本洗脱腔12的活塞5，同时上提对应裂解液腔10的活塞5，驱动反应腔36内的废液流入废液腔40内；清洗杂质完毕后，断开电磁铁，下压对应洗脱液腔17的活塞，同时上提对应裂解液腔10的活塞5，洗脱液流入反应腔36，以洗脱磁珠上的核酸；经过一定时间，洗脱核酸完毕后，接通电磁铁，将磁珠吸附在反应腔36的底部，关闭第三微阀44，打开第二微阀43和第四微阀45，下压对应气动腔19的活塞5，同时上提对应裂解液腔10的活塞5，驱动核酸流至核酸出口38。The working process of the nucleic acid extraction system described in the present application is briefly described as follows: when the first microvalve 42 is opened and the second microvalve 43, the third microvalve 44 and the fourth microvalve 45 are closed, the piston 5 corresponding to the lysis liquid chamber 10 is pressed down, and the piston 5 corresponding to the sample elution chamber 12 is lifted up at the same time, so as to drive the lysis liquid in the lysis liquid chamber 10 to flow into the sample elution chamber 12, and the liquid sample or swab is lysed and eluted to obtain the sample lysis product; after the lysis and elution are completed, the first microvalve 42 is closed, the third microvalve 44 is opened, the piston 5 corresponding to the sample elution chamber 12 is pressed down, and the piston 5 corresponding to the lysis liquid chamber 10 is lifted up at the same time, so as to drive the sample lysis product to flow into the reaction chamber 36, and the nucleic acid in the sample lysis product is captured by magnetic beads and binding reagents; after the nucleic acid capture is completed, the electromagnet is turned on to adsorb the magnetic beads at the bottom of the reaction chamber 36; the piston 5 corresponding to the sample elution chamber 12 is continued to be pressed down, and the piston 5 corresponding to the lysis liquid chamber 10 is lifted up at the same time, so as to drive the waste liquid in the reaction chamber 36 to flow into the waste liquid chamber 40. It should be noted that the piston 5 in the sample elution chamber 12 has a certain initial height. After the cover of the injection port 20 is opened, a certain amount of gas exists in the sample elution chamber 12, and the gas can be used as a driving force for the flow of liquid in subsequent operations. Disconnect the electromagnet, press down the piston 5 corresponding to the first cleaning liquid chamber 13, and at the same time lift up the piston 5 corresponding to the lysis liquid chamber 10, drive the first cleaning liquid to flow into the reaction chamber 36 to clean the impurities on the magnetic beads; connect the electromagnet to adsorb the magnetic beads at the bottom of the reaction chamber 36, continue to press down the piston 5 corresponding to the sample elution chamber 12, and at the same time lift up the piston 5 corresponding to the lysis liquid chamber 10, drive the waste liquid in the reaction chamber 36 to flow into the waste liquid chamber 40; disconnect the electromagnet, press down the piston 5 corresponding to the second cleaning liquid chamber 15, and at the same time lift up the piston 5 corresponding to the lysis liquid chamber 10, drive the second cleaning liquid to flow into the reaction chamber 36 to clean the impurities on the magnetic beads; connect the electromagnet to adsorb the magnetic beads at the bottom of the reaction chamber 36 ; Continue to press down the piston 5 corresponding to the sample elution chamber 12, and at the same time lift up the piston 5 corresponding to the lysis liquid chamber 10, so as to drive the waste liquid in the reaction chamber 36 to flow into the waste liquid chamber 40; after the impurities are cleaned, disconnect the electromagnet, press down the piston corresponding to the elution liquid chamber 17, and at the same time lift up the piston 5 corresponding to the lysis liquid chamber 10, so that the eluent flows into the reaction chamber 36 to elute the nucleic acid on the magnetic beads; after a certain period of time, when the nucleic acid is eluted, turn on the electromagnet to adsorb the magnetic beads at the bottom of the reaction chamber 36, close the third microvalve 44, open the second microvalve 43 and the fourth microvalve 45, press down the piston 5 corresponding to the pneumatic chamber 19, and at the same time lift up the piston 5 corresponding to the lysis liquid chamber 10, so as to drive the nucleic acid to flow to the nucleic acid outlet 38.
实施方式二Implementation Method 2
在搭载快速膜提取技术时，所述核酸捕获物为结合试剂和富集膜。如图11至图15所示的核酸提取系统的另一种具体实施方式。如图13至图15所示，所述样本洗脱腔12和试剂承载腔的底部均为平底，且横截面均为圆形；所述针头9的外端是平头。所述试剂承载腔包括：裂解腔、乙醇腔14、第一漂洗液腔16、第二漂洗液腔18和洗脱液腔17；消化缓冲液腔11即裂解腔。所述消化缓冲液腔11、样本洗脱腔12、乙醇腔14、第一漂洗液腔16和第二漂洗液腔18的直径尺寸一致，均为洗脱液腔17直径的二倍。在所述消化缓冲液腔11内存储有消化缓冲液，在所述乙醇腔14内存储有乙醇，在所述第一漂洗液腔16内存储有第一漂洗液，在所述第二漂洗液腔18内存储有第二漂洗液；在所述反应腔36内存储有结合试剂和富集膜，具体的，所述结合试剂为干化的结合试剂；在所述样本洗脱腔12内存有蛋白酶k，使用时，首先推动所述裂解腔内的活塞，将所述消化缓冲液推动至所述样本洗脱腔12内混合，然后将混合液推动至乙醇腔14内。在所述样本洗脱腔12外置有局部加热模块；所述消化缓冲液腔11、样本洗脱腔12、乙醇腔14、第一漂洗液腔16、第二漂洗液腔18和洗脱液腔17对应的试剂入口分别为：消化缓冲液入口27、样本入口28、乙醇入口30、第一漂洗液入口32、第二漂洗液入口34和洗脱液入口33；所述消化缓冲液入口27与样本入口28连通，所述样本入口28通过第一微阀42与反应腔36连通；所述消化缓冲液入口27与废液腔40通过蛇形防反流流道41连通；所述乙醇入口30、第一漂洗液入口32、第二漂洗液入口34、洗脱液入口33均与反应腔36连通。When the rapid membrane extraction technology is used, the nucleic acid capture object is a binding reagent and an enrichment membrane. Another specific embodiment of the nucleic acid extraction system shown in Figures 11 to 15. As shown in Figures 13 to 15, the bottoms of the sample elution chamber 12 and the reagent carrying chamber are flat-bottomed, and the cross-sections are circular; the outer end of the needle 9 is flat. The reagent carrying chamber includes: a lysis chamber, an ethanol chamber 14, a first rinsing liquid chamber 16, a second rinsing liquid chamber 18 and an elution liquid chamber 17; the digestion buffer chamber 11 is the lysis chamber. The diameters of the digestion buffer chamber 11, the sample elution chamber 12, the ethanol chamber 14, the first rinsing liquid chamber 16 and the second rinsing liquid chamber 18 are consistent, and are all twice the diameter of the elution liquid chamber 17. Digestion buffer is stored in the digestion buffer chamber 11, ethanol is stored in the ethanol chamber 14, a first rinsing liquid is stored in the first rinsing liquid chamber 16, and a second rinsing liquid is stored in the second rinsing liquid chamber 18; binding reagents and enrichment membranes are stored in the reaction chamber 36, specifically, the binding reagents are dried binding reagents; proteinase K is stored in the sample elution chamber 12. When in use, the piston in the lysis chamber is first pushed to push the digestion buffer into the sample elution chamber 12 for mixing, and then the mixed liquid is pushed into the ethanol chamber 14. A local heating module is arranged outside the sample elution chamber 12; the reagent inlets corresponding to the digestion buffer chamber 11, the sample elution chamber 12, the ethanol chamber 14, the first rinsing liquid chamber 16, the second rinsing liquid chamber 18 and the elution liquid chamber 17 are respectively: a digestion buffer inlet 27, a sample inlet 28, an ethanol inlet 30, a first rinsing liquid inlet 32, a second rinsing liquid inlet 34 and an elution liquid inlet 33; the digestion buffer inlet 27 is connected with the sample inlet 28, and the sample inlet 28 is connected with the reaction chamber 36 through the first microvalve 42; the digestion buffer inlet 27 is connected with the waste liquid chamber 40 through the serpentine anti-backflow channel 41; the ethanol inlet 30, the first rinsing liquid inlet 32, the second rinsing liquid inlet 34 and the elution liquid inlet 33 are all connected with the reaction chamber 36.
本申请所述核酸提取系统的工作过程简述如下：在打开第一微阀42，关闭第二微阀43、第三微阀44和第四微阀45时，下压对应消化缓冲液腔11的活塞5，同时上提对应样本洗脱腔12的活塞5，驱动消化缓冲液腔11中的消化缓冲液流入样本洗脱腔12内，消化缓冲液与液体样本或拭子，以及蛋白酶k进行混合，并在一定温度下孵育10min，对液体样本或拭子进行裂解洗脱，暴露核酸，获得样本裂解产物；在裂解洗脱完成后，下压对应样本洗脱腔12的活塞5，同时上提对应乙醇腔14的活塞5，驱动样本裂解产物流入乙醇腔14内，乙醇与样本裂解产物混合，去除水分，沉淀核酸；关闭第一微阀42，打开第三微阀44，下压对应乙醇腔14的活塞5，同时上提对应消化缓冲液腔11的活塞5，驱动乙醇腔14内的混合物流入反应腔36内，利用富集膜过滤吸附核酸；核酸吸附完成后，下压对应第一漂洗液腔16的活塞5，同时上提对应消化缓冲液腔11的活塞5，第一漂洗液流入反应腔36，驱动反应腔36内的废液流入废液腔40内，冲洗富集膜上的杂质；下压对应第二漂洗液腔18的活塞5，同时上提对应消化缓冲液腔11的活塞5，第二漂洗液流入反应腔36，驱动反应腔36内的废液流入废液腔40内，冲洗富集膜上的杂质；完成漂洗后，关闭第三微阀44，打开第二微阀43和第四微阀45，下压对应洗脱液腔17的活塞5，同时上提对应消化缓冲液腔11的活塞5，洗脱液流入反应腔36，以洗脱富集膜上的核酸，驱动核酸流至核酸出口38。The working process of the nucleic acid extraction system described in the present application is briefly described as follows: when the first microvalve 42 is opened and the second microvalve 43, the third microvalve 44 and the fourth microvalve 45 are closed, the piston 5 corresponding to the digestion buffer chamber 11 is pressed down, and the piston 5 corresponding to the sample elution chamber 12 is lifted up at the same time, so that the digestion buffer in the digestion buffer chamber 11 flows into the sample elution chamber 12, the digestion buffer is mixed with the liquid sample or swab, and the proteinase K, and incubated at a certain temperature for 10 minutes, the liquid sample or swab is lysed and eluted, the nucleic acid is exposed, and the sample lysate is obtained; after the lysate and elution are completed, the piston 5 corresponding to the sample elution chamber 12 is pressed down, and the piston 5 corresponding to the ethanol chamber 14 is lifted up at the same time, so that the sample lysate product flows into the ethanol chamber 14, the ethanol is mixed with the sample lysate product, the water is removed, and the nucleic acid is precipitated; the first microvalve 42 is closed, the third microvalve 44 is opened, the piston 5 corresponding to the ethanol chamber 14 is pressed down, and the piston 5 corresponding to the digestion buffer chamber 11 is lifted up at the same time, The piston 5 of the flushing liquid chamber 11 drives the mixture in the ethanol chamber 14 to flow into the reaction chamber 36, and the nucleic acid is adsorbed by filtering through the enrichment membrane; after the nucleic acid adsorption is completed, the piston 5 corresponding to the first rinsing liquid chamber 16 is pressed down, and the piston 5 corresponding to the digestion buffer chamber 11 is lifted up, the first rinsing liquid flows into the reaction chamber 36, and the waste liquid in the reaction chamber 36 is driven to flow into the waste liquid chamber 40 to wash the impurities on the enrichment membrane; the piston 5 corresponding to the second rinsing liquid chamber 18 is pressed down, and the piston 5 corresponding to the digestion buffer chamber 11 is lifted up, the second rinsing liquid flows into the reaction chamber 36, and the waste liquid in the reaction chamber 36 is driven to flow into the waste liquid chamber 40 to wash the impurities on the enrichment membrane; after the rinsing is completed, the third microvalve 44 is closed, the second microvalve 43 and the fourth microvalve 45 are opened, the piston 5 corresponding to the elution liquid chamber 17 is pressed down, and the piston 5 corresponding to the digestion buffer chamber 11 is lifted up, the elution liquid flows into the reaction chamber 36 to elute the nucleic acid on the enrichment membrane, and the nucleic acid is driven to flow to the nucleic acid outlet 38.
可替换的实施方式，在上述核酸提取系统中，在所述核酸提取系统仅包括：试剂仓8和提取操作组件2的基础上，所述核酸捕获物为结合试剂和富集膜。通过反应腔36内的结合试剂和富集膜，将核酸捕获至富集膜上。In an alternative embodiment, in the above nucleic acid extraction system, the nucleic acid extraction system only includes: the reagent compartment 8 and the extraction operation component 2, and the nucleic acid capture object is a binding reagent and an enrichment membrane. The nucleic acid is captured on the enrichment membrane by the binding reagent and the enrichment membrane in the reaction chamber 36.
可替换的实施方式，在上述核酸提取系统中，在所述核酸提取系统仅包括：试剂仓8和提取操作组件2，以及所述核酸捕获物为结合试剂和富集膜的基础上，所述试剂承载腔包括：裂解腔和乙醇腔14，所述裂解腔内设有消化缓冲液，在所述乙醇腔14内存储有乙醇，在所述样本洗脱腔12内存有蛋白酶k；使用时，首先推动所述裂解腔内的活塞，将所述消化缓冲液推动至所述样本洗脱腔12内混合，然后将混合液推动至乙醇腔14内。利用消化缓冲液结合蛋白酶k裂解样本，然后再利用乙醇对裂解后的样本进行脱水，最后将裂解脱水后的产物在反应腔36内，利用富集膜捕获核酸。In an alternative embodiment, in the above nucleic acid extraction system, the nucleic acid extraction system only includes: a reagent compartment 8 and an extraction operation component 2, and the nucleic acid capture object is a combination reagent and an enrichment membrane, and the reagent carrying chamber includes: a lysis chamber and an ethanol chamber 14, a digestion buffer is provided in the lysis chamber, ethanol is stored in the ethanol chamber 14, and proteinase K is stored in the sample elution chamber 12; when in use, first push the piston in the lysis chamber, push the digestion buffer into the sample elution chamber 12 for mixing, and then push the mixed solution into the ethanol chamber 14. The sample is lysed by using the digestion buffer combined with proteinase K, and then the lysed sample is dehydrated by using ethanol, and finally the lysed and dehydrated product is placed in the reaction chamber 36, and the nucleic acid is captured by using the enrichment membrane.
其他技术方案与实施方式一相同。Other technical solutions are the same as those in implementation mode 1.
显然，上述实施例仅仅是为清楚地说明所作的举例，而并非对实施方式的限定。对于所属领域的普通技术人员来说，在上述说明的基础上还可以做出其他不同形式的变化或变动。这里无需也无法对所有的实施方式予以穷举。而由此所引申出的显而易见的变化或变动仍处于本发明创造的保护范围之中。Obviously, the above embodiments are merely examples for clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from them are still within the protection scope of the invention.
| Application Number | Priority Date | Filing Date | Title |
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| CN202410383050.6ACN118185730A (en) | 2024-03-29 | 2024-03-29 | A nucleic acid extraction system |
| Application Number | Priority Date | Filing Date | Title |
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| CN202410383050.6ACN118185730A (en) | 2024-03-29 | 2024-03-29 | A nucleic acid extraction system |
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| CN118185730Atrue CN118185730A (en) | 2024-06-14 |
| Application Number | Title | Priority Date | Filing Date |
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| CN202410383050.6APendingCN118185730A (en) | 2024-03-29 | 2024-03-29 | A nucleic acid extraction system |
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|---|---|
| CN (1) | CN118185730A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119144431A (en)* | 2024-09-11 | 2024-12-17 | 中国科学院过程工程研究所 | Nucleic acid integrated detection device based on flexible structure and push-pull structure |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257245A (en)* | 2019-07-16 | 2019-09-20 | 东莞博识生物科技有限公司 | Nucleic acid detection reagent card |
| CN217499275U (en)* | 2022-05-19 | 2022-09-27 | 北京中科生仪科技有限公司 | Totally-enclosed nucleic acid detection chip |
| CN115703993A (en)* | 2021-08-17 | 2023-02-17 | 北京京东方技术开发有限公司 | Micro-fluidic chip, nucleic acid extraction method thereof and nucleic acid extraction device |
| CN115820412A (en)* | 2022-11-17 | 2023-03-21 | 北京昌平实验室 | A microfluidic cartridge and method for specific detection of nucleic acid from complex samples |
| WO2023097922A1 (en)* | 2021-11-30 | 2023-06-08 | 珠海黑马生物科技有限公司 | Fully-enclosed fully-automatic nucleic acid extraction kit |
| CN219429977U (en)* | 2023-03-23 | 2023-07-28 | 郑州中科生物医学工程技术研究院 | Nucleic acid extraction kit and nucleic acid extraction device |
| CN116790366A (en)* | 2023-07-06 | 2023-09-22 | 集美大学 | A plunger-type fully automatic nucleic acid extraction device and its control method |
| WO2023206096A1 (en)* | 2022-04-26 | 2023-11-02 | 广州国家实验室 | Liquid transfer device and multi-path parallel liquid transfer device |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257245A (en)* | 2019-07-16 | 2019-09-20 | 东莞博识生物科技有限公司 | Nucleic acid detection reagent card |
| CN115703993A (en)* | 2021-08-17 | 2023-02-17 | 北京京东方技术开发有限公司 | Micro-fluidic chip, nucleic acid extraction method thereof and nucleic acid extraction device |
| WO2023097922A1 (en)* | 2021-11-30 | 2023-06-08 | 珠海黑马生物科技有限公司 | Fully-enclosed fully-automatic nucleic acid extraction kit |
| WO2023206096A1 (en)* | 2022-04-26 | 2023-11-02 | 广州国家实验室 | Liquid transfer device and multi-path parallel liquid transfer device |
| CN217499275U (en)* | 2022-05-19 | 2022-09-27 | 北京中科生仪科技有限公司 | Totally-enclosed nucleic acid detection chip |
| CN115820412A (en)* | 2022-11-17 | 2023-03-21 | 北京昌平实验室 | A microfluidic cartridge and method for specific detection of nucleic acid from complex samples |
| CN219429977U (en)* | 2023-03-23 | 2023-07-28 | 郑州中科生物医学工程技术研究院 | Nucleic acid extraction kit and nucleic acid extraction device |
| CN116790366A (en)* | 2023-07-06 | 2023-09-22 | 集美大学 | A plunger-type fully automatic nucleic acid extraction device and its control method |
| Title |
|---|
| 普冬;郝宏刚;郭军辉;: "全自动核酸纯化系统在临床分子检测中的应用研究", 中国热带医学, no. 10, 18 October 2013 (2013-10-18), pages 1282 - 1285* |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119144431A (en)* | 2024-09-11 | 2024-12-17 | 中国科学院过程工程研究所 | Nucleic acid integrated detection device based on flexible structure and push-pull structure |
| Publication | Publication Date | Title |
|---|---|---|
| CN112226361B (en) | Nucleic acid detection card box based on magnetic bead transfer and valve control liquid transfer and detection method | |
| CN110075935B (en) | Multi-index detection microfluidic cartridge and application method | |
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| RJ01 | Rejection of invention patent application after publication | Application publication date:20240614 | |
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