技术领域Technical Field
本发明涉及化妆品技术领域，尤其是涉及一种含茶多酚的协同增效修复组合物及其应用。The invention relates to the technical field of cosmetics, and in particular to a synergistic repairing composition containing tea polyphenols and application thereof.
背景技术Background technique
UVB为中波紫外线，其波长波段为275nm至320nm，阳光中只有约2％的UVB可以到达地球表面，适当的UVB照射能够能促进人体内矿物质代谢和维生素D的形成，但UVB照射还会引起皮肤发红、脱皮皮肤和加速皮肤老化等问题，因此如何在接受阳光照射的同时还能保护皮肤不受到损伤备受本领域人员关注。UVB is medium-wave ultraviolet light with a wavelength range of 275nm to 320nm. Only about 2% of UVB in sunlight can reach the earth's surface. Appropriate UVB exposure can promote mineral metabolism and the formation of vitamin D in the human body, but UVB exposure can also cause skin redness, peeling, and accelerated skin aging. Therefore, how to protect the skin from damage while receiving sunlight exposure has attracted much attention from people in this field.
目前解决上述问题的主要方式通过在皮肤表面涂附修复层，利用修复层中的有效成分增强皮肤抵抗和修复紫外线的刺激作用，目前已公开的有效成分有神经酰胺和茶多酚等；如中国专利CN104173207B公开的一种皮肤屏障修复剂，该修复剂采用了三种神经酰胺复配，对皮肤的水分、油分和经皮水分流失都具有较好的效果，尤其经皮水分流失值大幅度降低，对皮肤屏障修复有着显著的效果；再如中国专利CN102813613B公开了一种具有防晒修复的湿巾产品，该产品采用天然茶多酚对皮肤起到温和防晒和修复的作用；但该产品并未公开茶多酚的作用机理。At present, the main way to solve the above problems is to apply a repair layer on the skin surface and use the effective ingredients in the repair layer to enhance the skin's resistance to and repair the stimulation of ultraviolet rays. The effective ingredients currently disclosed include ceramides and tea polyphenols. For example, Chinese patent CN104173207B discloses a skin barrier repair agent, which uses a compound of three ceramides and has good effects on the moisture, oil and transepidermal water loss of the skin, especially a significant reduction in the transepidermal water loss value, which has a significant effect on skin barrier repair. Another example is Chinese patent CN102813613B discloses a wet wipes product with sunscreen and repairing properties. The product uses natural tea polyphenols to gently protect and repair the skin. However, the product does not disclose the mechanism of action of tea polyphenols.
目前茶多酚主要应用在皮肤抗衰老和皮肤保湿等场景，且茶多酚常与泛醇配合使用，如公开号CN111557882A公开的一种具有抗衰老的组合物，该组合物包括茶多酚和泛醇等组分，该组合物可减少/延缓胶原蛋白的分解、提高皮肤含水量及清除自由基等发挥抗衰老的效果；再如公开号CN114748366A公开的一种保湿身体护理霜，该产品中包含棕榈酸乙基己酯、泛醇、尿囊素、茶多酚等组分，该产品通过减少经皮水分的流失达到保湿滋润肌肤的技术效果；目前茶多酚与泛醇的组合物在皮肤抗衰老和皮肤保湿等领域中应用广泛，但在抗UVB辐射中的应用还未见报道，因此茶多酚与泛醇的组合物在抗UVB辐射中的作用效果还有待探究。At present, tea polyphenols are mainly used in scenarios such as skin anti-aging and skin moisturizing, and tea polyphenols are often used in combination with panthenol. For example, Publication No. CN111557882A discloses an anti-aging composition, which includes components such as tea polyphenols and panthenol. The composition can reduce/delay the decomposition of collagen, increase skin water content, and scavenge free radicals to exert anti-aging effects; another example is a moisturizing body care cream disclosed in Publication No. CN114748366A, which contains components such as ethylhexyl palmitate, panthenol, allantoin, tea polyphenols, etc. The product achieves the technical effect of moisturizing the skin by reducing transepidermal water loss; currently, the combination of tea polyphenols and panthenol is widely used in the fields of skin anti-aging and skin moisturizing, but its application in anti-UVB radiation has not been reported, so the effect of the combination of tea polyphenols and panthenol in anti-UVB radiation remains to be explored.
发明内容Summary of the invention
本发明为了探究茶多酚和泛醇的组合物在UVB辐射皮肤场景下的作用，提供了一种含茶多酚的协同增效修复组合物及其应用，该组合物由茶多酚和泛醇组成，该组合物能够有效抑制UVB辐射对皮肤的损伤，且该组合物的存在协同增效作用，该组合物的协同作用效果显著高于神经酰胺、茶多酚、泛醇以及茶多酚/神经酰胺组合物的作用效果。In order to explore the effect of a combination of tea polyphenols and panthenol under UVB radiation skin scenarios, the present invention provides a synergistic repair composition containing tea polyphenols and applications thereof. The composition consists of tea polyphenols and panthenol, and the composition can effectively inhibit the damage of UVB radiation to the skin. The composition has a synergistic effect, and the synergistic effect of the composition is significantly higher than the effects of ceramide, tea polyphenols, panthenol and the tea polyphenol/ceramide combination.
本发明的具体技术方案为：The specific technical scheme of the present invention is:
一种含茶多酚的协同增效修复组合物，包括质量份数比为0.001~0.01：1的茶多酚和D-泛醇。A synergistic repair composition containing tea polyphenols comprises tea polyphenols and D-panthenol in a mass ratio of 0.001-0.01:1.
本发明提供了一种含茶多酚的协同增效修复组合物，该组合物由茶多酚和D-泛醇组成，该组合物能够有效抑制UVB辐射对皮肤细胞的损伤作用，在UVB辐射处理皮肤细胞时，使用该组合物能够显著降低皮肤细胞超氧化物歧化酶的活性，能够显著提高皮肤细胞线粒体膜电位，能够显著降低皮肤细胞的炎症因子，且该组合物具有协同增效的作用，该组合物与使用茶多酚、D-泛醇和神经、神经酰胺以及神经酰胺/茶多酚组合相比作用效果更加显著。The invention provides a synergistic repair composition containing tea polyphenols. The composition consists of tea polyphenols and D-panthenol. The composition can effectively inhibit the damaging effect of UVB radiation on skin cells. When the skin cells are treated with UVB radiation, the use of the composition can significantly reduce the activity of superoxide dismutase of the skin cells, significantly increase the mitochondrial membrane potential of the skin cells, and significantly reduce the inflammatory factors of the skin cells. The composition has a synergistic effect. Compared with the use of tea polyphenols, D-panthenol and ceramide, ceramide and ceramide/tea polyphenol combination, the effect of the composition is more significant.
作为优选，含茶多酚的协同增效修复组合物协同抑制UVB辐射对细胞的损伤作用。Preferably, the synergistic repair composition containing tea polyphenols synergistically inhibits the damaging effect of UVB radiation on cells.
一种上述含茶多酚的协同增效修复组合物在面霜中的应用，面霜的原料包括A组分、B组分、C组分和D组分；所述D组分为权利要求1中的含茶多酚的协同增效修复组合物，所述D组分在面霜中的质量百分数为0.05~10%。An application of the above-mentioned synergistic repair composition containing tea polyphenols in a facial cream, wherein the raw materials of the facial cream include component A, component B, component C and component D; the component D is the synergistic repair composition containing tea polyphenols in claim 1, and the mass percentage of the component D in the facial cream is 0.05-10%.
本发明还提供了上述含茶多酚的协同增效修复组合物在化妆品中的应用，该化妆品的制剂形式为面霜，该化妆品中的有效成分为上述含茶多酚的协同增效修复组合物，该化妆品对皮肤温和无刺激，不仅能够抑制UVB辐射对皮肤的损伤作用，同时还具有舒缓皮肤不适、改善皮肤粗糙、柔嫩光滑肌肤的作用。The present invention also provides the use of the above-mentioned synergistic repair composition containing tea polyphenols in cosmetics. The preparation form of the cosmetics is a facial cream. The active ingredient in the cosmetics is the above-mentioned synergistic repair composition containing tea polyphenols. The cosmetics are mild and non-irritating to the skin, and can not only inhibit the damaging effect of UVB radiation on the skin, but also have the effects of soothing skin discomfort, improving rough skin, and softening and smoothing the skin.
作为优选，所述A组分按质量份数计包括：甘油2~12份，增稠剂0.1~0.8份，螯合剂0.1~1份，pH调节剂0.002~0.05份。Preferably, the component A comprises, by mass, 2 to 12 parts of glycerol, 0.1 to 0.8 parts of thickener, 0.1 to 1 parts of chelating agent, and 0.002 to 0.05 parts of pH adjuster.
作为优选，所述增稠剂包括卡波姆、聚丙烯酸酯交联聚合物-6、黄原胶、丙烯酸（酯）类/共聚物、丙烯酸（酯）类/C10-30烷醇丙烯酸交联聚合物、羟乙基纤维素、小核菌胶和聚丙烯酸钠中的一种或多种；Preferably, the thickener comprises one or more of carbomer, polyacrylate crosspolymer-6, xanthan gum, acrylic acid (esters)/copolymer, acrylic acid (esters)/C10-30 alkyl acrylic acid crosspolymer, hydroxyethyl cellulose, sclerotium gum and sodium polyacrylate;
所述螯合剂包括乙二胺四乙酸二钠、植酸钠和柠檬酸钠中的一种或多种；The chelating agent includes one or more of disodium edetate, sodium phytate and sodium citrate;
所述pH调节剂包括精氨酸、柠檬酸、乳酸、乳酸钠和柠檬酸钠中一种或多种。The pH regulator includes one or more of arginine, citric acid, lactic acid, sodium lactate and sodium citrate.
作为优选，所述B组分按质量份数计包括：乳化剂1~5份，植物油2~15份，合成油脂2~10份，鲸蜡硬脂醇1~5份，抗氧化剂0.2~1份。Preferably, the component B comprises, by mass, 1 to 5 parts of emulsifier, 2 to 15 parts of vegetable oil, 2 to 10 parts of synthetic oil, 1 to 5 parts of cetearyl alcohol, and 0.2 to 1 part of antioxidant.
作为优选，所述乳化剂包括聚甘油-6硬质酸钠、聚甘油-6二硬脂酸酯、甘油硬脂酸柠檬酸酯、硬脂酰谷氨酸钠、甘油硬脂酸酯、鲸蜡醇磷酸酯钾和硬脂醇聚醚-21中的一种或多种；Preferably, the emulsifier includes one or more of polyglyceryl-6 sodium stearate, polyglyceryl-6 distearate, glyceryl stearate citrate, sodium stearoyl glutamate, glyceryl stearate, potassium cetyl phosphate and steareth-21;
作为优选，所述植物油包括山茶油、高油酸向日葵籽油、亚麻籽油、白池花籽油、葡萄籽油和鳄梨油中的一种或多种；Preferably, the vegetable oil comprises one or more of camellia oil, high oleic sunflower seed oil, linseed oil, meadowfoam seed oil, grape seed oil and avocado oil;
作为优选，所述合成油脂包括氢化聚异丁烯、辛酸/癸酸甘油三酯、二异硬脂醇苹果酸酯、角鲨烷、聚二甲基硅氧烷、异十二烷和棕榈酸乙基己酯中的一种或多种；Preferably, the synthetic oil comprises one or more of hydrogenated polyisobutene, caprylic/capric triglyceride, diisostearyl malate, squalane, polydimethylsiloxane, isododecane and ethylhexyl palmitate;
作为优选，所述抗氧化剂选自生育酚、生育酚乙酸酯、抗氧化剂1010、丁羟甲苯和丁羟茴醚中的一种或多种。Preferably, the antioxidant is selected from one or more of tocopherol, tocopherol acetate, antioxidant 1010, butylated hydroxytoluene and butylated hydroxyanisole.
作为优选，所述C组分按质量份数计包括：防腐剂0.1~3份，日用香精0.01~1份。Preferably, the C component comprises, by mass, 0.1 to 3 parts of preservatives and 0.01 to 1 parts of daily flavors.
作为优选，所述防腐剂选自对羟基苯乙酮、戊二醇、1，2-己二醇、苯氧乙醇、辛甘醇、氯苯甘醚、乙基己基甘油、苯甲酸钠、山梨酸钾和羟基苯丙酮中的一种或多种。Preferably, the preservative is selected from one or more of p-hydroxyacetophenone, pentylene glycol, 1,2-hexanediol, phenoxyethanol, caprylyl glycol, chlorphenesin, ethylhexylglycerin, sodium benzoate, potassium sorbate and hydroxypropiophenone.
作为优选，所述面霜的制备方法包括以下步骤：将A组分各原料混匀并加热，加热至65~75 ℃，混匀10~20 min；将B组分各原料混匀并加热至完全溶解，加热至65~75 ℃；将B组分与A组分混合均匀，65~75 ℃下均质8~10 min，降低温度至60 ℃以下加入C组分并混匀，温度继续下降至45 ℃以下加入D组分并混匀，温度再次下降至37 ℃以下制得协同增效修复皮肤面霜。Preferably, the preparation method of the facial cream comprises the following steps: mixing and heating the raw materials of component A to 65-75°C and mixing for 10-20 min; mixing and heating the raw materials of component B until completely dissolved and heating to 65-75°C; mixing component B and component A evenly, homogenizing at 65-75°C for 8-10 min, lowering the temperature to below 60°C, adding component C and mixing, continuing to lower the temperature to below 45°C, adding component D and mixing, and lowering the temperature again to below 37°C to obtain a synergistic skin repairing cream.
与现有技术相比，本发明具有以下技术效果：Compared with the prior art, the present invention has the following technical effects:
（1）本发明提供的含茶多酚的协同增效修复组合物能够有效抑制UVB辐射对皮肤细胞的损伤作用，在UVB辐射处理皮肤细胞时，使用该组合物能够显著降低皮肤细胞超氧化物歧化酶的活性，能够显著提高皮肤细胞线粒体膜电位，能够显著降低皮肤细胞的炎症因子，且该组合物具有协同增效的作用，该组合物与使用茶多酚、D-泛醇和神经酰胺以及神经酰胺/茶多酚组合物相比作用效果更加显著；(1) The synergistic repair composition containing tea polyphenols provided by the present invention can effectively inhibit the damaging effect of UVB radiation on skin cells. When skin cells are treated with UVB radiation, the use of the composition can significantly reduce the activity of superoxide dismutase in skin cells, significantly increase the mitochondrial membrane potential of skin cells, and significantly reduce the inflammatory factors of skin cells. The composition has a synergistic effect. The effect of the composition is more significant than that of tea polyphenols, D-panthenol and ceramide, and ceramide/tea polyphenols composition.
（2）本发明提供的有效成分为含茶多酚的协同增效修复组合物的面霜，不仅能够抑制UVB辐射对皮肤的损伤作用，还能够舒缓皮肤不适、改善皮肤粗糙以及柔嫩光滑肌肤，且该面霜温和安全无刺激。(2) The facial cream provided by the present invention has a synergistic repairing composition containing tea polyphenols as an active ingredient, which can not only inhibit the damaging effect of UVB radiation on the skin, but also relieve skin discomfort, improve skin roughness, and make the skin tender and smooth. The facial cream is mild, safe and non-irritating.
附图说明BRIEF DESCRIPTION OF THE DRAWINGS
图1为本发明实施例1和对比例1至7的细胞内超氧化物歧化酶的活性百分率图。FIG. 1 is a graph showing the percentage of intracellular superoxide dismutase activity in Example 1 of the present invention and Comparative Examples 1 to 7.
图2为本发明实施例1和对比例1至7的细胞内线粒体膜电位图。FIG. 2 is a diagram of intracellular mitochondrial membrane potential of Example 1 of the present invention and Comparative Examples 1 to 7.
图3为本发明实施例1和对比例1至7的细胞内IL-6浓度示意图。FIG3 is a schematic diagram of intracellular IL-6 concentrations in Example 1 of the present invention and Comparative Examples 1 to 7.
图4为本发明实施例2和对比例8的保湿效果图。FIG. 4 is a diagram showing the moisturizing effects of Example 2 of the present invention and Comparative Example 8.
图5为本发明实施例2和对比例8的皮肤经表皮流失TWEL值图。FIG. 5 is a graph showing the transepidermal skin loss TWEL values of Example 2 of the present invention and Comparative Example 8.
图6为本发明实施例2和对比例8的使用者评价图。FIG. 6 is a user evaluation diagram of Example 2 of the present invention and Comparative Example 8.
具体实施方式Detailed ways
下面结合实施例对本发明作进一步的描述。The present invention will be further described below in conjunction with embodiments.
实施例1：Embodiment 1:
一种含茶多酚的协同增效修复组合物，包括10 µg/mL的茶多酚和100 µg/mL的D-泛醇。A synergistic repairing composition containing tea polyphenols comprises 10 µg/mL of tea polyphenols and 100 µg/mL of D-panthenol.
对比例1：Comparative Example 1:
与实施例1相比，对比例1仅含有10 µg/mL的茶多酚。Compared with Example 1, Comparative Example 1 contained only 10 µg/mL of tea polyphenols.
对比例2：Comparative Example 2:
与实施例1相比，对比例2仅含有100 µg/mL的茶多酚。Compared with Example 1, Comparative Example 2 contained only 100 µg/mL of tea polyphenols.
对比例3：Comparative Example 3:
与实施例1相比，对比例3仅含有100 µg/mL的D-泛醇。Compared with Example 1, Comparative Example 3 contained only 100 µg/mL of D-panthenol.
对比例4：Comparative Example 4:
与实施例1相比，对比例4仅含有1000 µg/mL的D-泛醇。Compared with Example 1, Comparative Example 4 contained only 1000 µg/mL of D-panthenol.
对比例5：Comparative Example 5:
与实施例1相比，对比例5仅含有100 µg/mL的神经酰胺。Compared with Example 1, Comparative Example 5 only contained 100 µg/mL of ceramide.
对比例6：Comparative Example 6:
与实施例1相比，对比例6仅含有1000 µg/mL的神经酰胺。Compared with Example 1, Comparative Example 6 only contained 1000 µg/mL of ceramide.
对比例7：Comparative Example 7:
与实施例1相比，对比例7含有10 µg/mL的茶多酚和100 µg/mL的神经酰胺。Compared with Example 1, Comparative Example 7 contains 10 µg/mL of tea polyphenols and 100 µg/mL of ceramide.
检测例1：Test example 1:
在UVB辐射条件下测试实施例1和对比例1至7对人类永生化表皮细胞（HACAT细胞）的细胞内SOD活性、细胞内膜电位和细胞内白细胞介素-6（IL-6）的影响；The effects of Example 1 and Comparative Examples 1 to 7 on intracellular SOD activity, intracellular membrane potential and intracellular interleukin-6 (IL-6) of human immortalized epidermal cells (HACAT cells) were tested under UVB radiation conditions;
UVB辐射剂量优选：将HaCaT细胞培养24小时，用不同辐照剂量的UVB辐照后继续培养24h，利用CCK8法检测细胞增值率，测试结果获得，辐射剂量为0 mJ/cm2时细胞增值率为100％，辐射剂量为10 mJ/cm2时细胞增值率为70％，辐射剂量为50 mJ/cm2时细胞增值率为60％，辐射剂量为100 mJ/cm2时细胞增值率为50％，辐射剂量为120 mJ/cm2时细胞增值率为40％，最终选择 100 mJ/cm2作为后续的辐照剂量。Optimization of UVB radiation dose: HaCaT cells were cultured for 24 hours, irradiated with UVB at different irradiation doses and then continued to be cultured for 24 hours. The cell proliferation rate was detected by CCK8 method. The test results showed that the cell proliferation rate was 100% when the radiation dose was 0 mJ/cm2 , 70% when the radiation dose was 10 mJ/cm2 , 60% when the radiation dose was 50 mJ/cm2 , 50% when the radiation dose was 100 mJ/cm2 , and 40% when the radiation dose was 120 mJ/cm2 . Finally, 100 mJ/cm2 was selected as the subsequent irradiation dose.
细胞毒性测试：将实施例1和对比例1至7加入到HACAT细胞培养液中，利用细胞培养增殖后检测不同样品对细胞增值率的影响，结果显示所有样品的细胞存活率均大于60％，结果表面实施例1和对比例1至7均可用于体外功效评价测试；Cytotoxicity test: Example 1 and Comparative Examples 1 to 7 were added to HACAT cell culture fluid, and the effects of different samples on cell proliferation rate were detected after cell culture proliferation. The results showed that the cell survival rates of all samples were greater than 60%, indicating that Example 1 and Comparative Examples 1 to 7 can be used for in vitro efficacy evaluation tests;
细胞内SOD活性的测试方法：设置空白对照组（不经过UVB辐照、不加样品）、UVB辐射模型组（100 J/cm2UVB辐照细胞，不加样品）和实验组（100 J/cm2UVB辐照细胞，实施例1和对比例1至7处理HACAT细胞培养基）；Test method for intracellular SOD activity: setting up a blank control group (no UVB irradiation, no sample added), a UVB radiation model group (100 J/cm2 UVB irradiated cells, no sample added) and an experimental group (100 J/cm2 UVB irradiated cells, HACAT cell culture medium treated with Example 1 and Comparative Examples 1 to 7);
利用超氧化物歧化酶测定试剂盒中的测定方法，检测空白对照组、辐照组及实验组的细胞内超氧化物歧化酶的活性变化，测试结果见图1；The superoxide dismutase assay kit was used to detect the changes in the activity of superoxide dismutase in the cells of the blank control group, irradiation group and experimental group. The test results are shown in Figure 1;
UVB辐照能明显降低细胞超氧化物歧化酶的活性，与辐照组相比，实施例1和对比例1至7均能提高超氧化物歧化酶活性的能力，从使用单一成分结果可知，泛醇（对比例3和4）抑制超氧化物歧化酶活性的能力显著高于茶多酚（对比例1和2）和神经酰胺（对比例5和6），从复配组结果可知，本发明的含茶多酚的协同增效修复组合物（实施例1）抑制超氧化物歧化酶活性的能力显著高于茶多酚/神经酰胺组合物，且显著高于其他单一成分组，上述结果表明，在UVB辐射皮肤细胞条件下，本发明提供的含茶多酚的协同增效修复组合物可以协同抑制皮肤细胞内超氧化物歧化酶的活性，提高皮肤细胞的抗氧化能力。UVB irradiation can significantly reduce the activity of superoxide dismutase in cells. Compared with the irradiation group, Example 1 and Comparative Examples 1 to 7 can all improve the ability of superoxide dismutase activity. From the results of using a single component, it can be seen that the ability of panthenol (Comparative Examples 3 and 4) to inhibit superoxide dismutase activity is significantly higher than that of tea polyphenols (Comparative Examples 1 and 2) and ceramide (Comparative Examples 5 and 6). From the results of the compound group, it can be seen that the ability of the synergistic repair composition containing tea polyphenols of the present invention (Example 1) to inhibit superoxide dismutase activity is significantly higher than that of the tea polyphenols/ceramide composition, and significantly higher than that of other single component groups. The above results show that under the condition of UVB irradiation of skin cells, the synergistic repair composition containing tea polyphenols provided by the present invention can synergistically inhibit the activity of superoxide dismutase in skin cells and improve the antioxidant capacity of skin cells.
利用线粒体膜电位检测试剂盒中的测定方法，用流式细胞仪检测线粒体膜电位变化情况，测试结果见图2；Using the determination method in the mitochondrial membrane potential detection kit, the change of mitochondrial membrane potential was detected by flow cytometry, and the test results are shown in Figure 2;
细胞线粒体的膜电位下降是细胞早期损伤的标志，如图2所示，UVB 使细胞中的线粒体膜电位降低，造成了细胞损伤；和辐照组相比，实施例1和对比例1至7均具有提高线粒体膜电位的能力；从使用单一成分结果可知，茶多酚（对比例1和2）提高线粒体膜电位的能力优于泛醇（对比例3和4）和神经酰胺（对比例5和6），从复配组结果可知，本发明的含茶多酚的协同增效修复组合物（实施例1）提高线粒体膜电位的效果显著高于茶多酚/神经酰胺组合物，且显著高于其他单一成分组，上述结果表明，在UVB辐射皮肤细胞条件下，本发明提供的含茶多酚的协同增效修复组合物可以协同提升皮肤细胞内线粒体膜电位，显著提高皮肤细胞对UVB辐射损伤的抵抗作用。The decrease in the membrane potential of cell mitochondria is a sign of early cell damage. As shown in Figure 2, UVB reduces the mitochondrial membrane potential in cells, causing cell damage. Compared with the irradiation group, Example 1 and Comparative Examples 1 to 7 all have the ability to increase the mitochondrial membrane potential. From the results of using a single component, it can be seen that the ability of tea polyphenols (Comparative Examples 1 and 2) to increase mitochondrial membrane potential is better than that of panthenol (Comparative Examples 3 and 4) and ceramide (Comparative Examples 5 and 6). From the results of the compound group, it can be seen that the synergistic repair composition containing tea polyphenols of the present invention (Example 1) has a significantly higher effect on increasing mitochondrial membrane potential than the tea polyphenols/ceramide composition, and is significantly higher than other single component groups. The above results show that under the condition of UVB irradiation of skin cells, the synergistic repair composition containing tea polyphenols provided by the present invention can synergistically increase the mitochondrial membrane potential in skin cells, and significantly improve the resistance of skin cells to UVB radiation damage.
利用IL-6测定试剂盒中的测定方法，检测细胞内IL-6的水平，测试结果见图3；The level of intracellular IL-6 was detected using the assay method in the IL-6 assay kit, and the test results are shown in Figure 3;
IL-6是细胞内的一种促炎因子，能减轻细胞的炎症反应，在炎症中起到关键作用，如图3所示，UVB使细胞中IL-6的浓度显著升高，与辐照组相比，实施例1和对比例1至7均具有降低细胞内IL-6浓度的能力；此外，100 μg/ml的茶多酚（对比例2）和本发明含茶多酚的协同增效修复组合物（实施例1）降低细胞内IL-6浓度的能力显著高于其他实验组（对比例1、3至6和对比例7），从上述结果可知，UVB辐射皮肤细胞条件下，本发明的组合物和高浓度的茶多酚（对比例2）相比，两者对皮肤细胞内IL-6的抑制效果相当且抑制效果显著高于单独使用的低浓度茶多酚（对比例1）、泛醇（对比例3和对比例4）、神经酰胺（对比例5和对比例6）以及神经酰胺/茶多酚复配组合（对比例7）；此外，从上述结果中还能看出泛醇能够显著降低茶多酚的使用量。IL-6 is a pro-inflammatory factor in cells, which can reduce the inflammatory response of cells and play a key role in inflammation. As shown in FIG3 , UVB significantly increases the concentration of IL-6 in cells. Compared with the irradiated group, Example 1 and Comparative Examples 1 to 7 all have the ability to reduce the concentration of IL-6 in cells; in addition, 100 μg/ml of tea polyphenols (Comparative Example 2) and the synergistic repair composition containing tea polyphenols of the present invention (Example 1) have significantly higher abilities to reduce the concentration of IL-6 in cells than other experimental groups (Comparative Examples 1, 3 to 6 and Comparative Example 7). From the above results, it can be seen that under the condition of UVB irradiation of skin cells, the composition of the present invention and the high concentration of tea polyphenols (Comparative Example 2) have comparable inhibitory effects on IL-6 in skin cells and the inhibitory effects are significantly higher than those of low concentration tea polyphenols (Comparative Example 1), panthenol (Comparative Example 3 and Comparative Example 4), ceramide (Comparative Example 5 and Comparative Example 6) and the ceramide/tea polyphenols compound combination (Comparative Example 7) used alone; in addition, it can be seen from the above results that panthenol can significantly reduce the usage of tea polyphenols.
综上所述，本发明提供的由茶多酚和D-泛醇组成的含茶多酚的协同增效修复组合物能显著增强超氧化物歧化酶的活性，能显著提高线粒体膜电位，能显著降低炎症因子的浓度，且存在协同增效作用，此外本发明提供的含茶多酚的协同增效修复组合物与单一成分相比，用量少且作用效果更加显著。In summary, the synergistic repair composition containing tea polyphenols and D-panthenol provided by the present invention can significantly enhance the activity of superoxide dismutase, significantly increase the mitochondrial membrane potential, significantly reduce the concentration of inflammatory factors, and has a synergistic effect. In addition, compared with a single component, the synergistic repair composition containing tea polyphenols provided by the present invention has a small dosage and a more significant effect.
实施例2：Embodiment 2:
一种含茶多酚的协同增效修复组合物面霜，该面霜的有效成分为上述含茶多酚的协同增效修复组合物，该面霜按质量分数计包括以下组分：甘油8%，增稠剂（卡波姆980）0.3%，螯合剂（柠檬酸钠）0.3%，pH调节剂（柠檬酸）0.03%，乳化剂（甘油硬脂酸柠檬酸酯1%，甘油硬脂酸酯0.5%）1.5%，植物油（山茶油）5%，合成油脂（聚二甲基硅氧烷3%+角鲨烷3%）6%，抗氧化剂（生育酚）0.2%，防腐剂（苯氧乙醇0.4%，乙基己基甘油0.1%）0.5%和余量水；A tea polyphenol-containing synergistic repair composition face cream, the active ingredient of the face cream is the above tea polyphenol-containing synergistic repair composition, and the face cream comprises the following components by mass fraction: 8% glycerin, 0.3% thickener (carbomer 980), 0.3% chelating agent (sodium citrate), 0.03% pH regulator (citric acid), 1.5% emulsifier (1% glyceryl stearate citrate, 0.5% glyceryl stearate), 5% vegetable oil (camellia oil), 6% synthetic oil (3% polydimethylsiloxane + 3% squalane), 0.2% antioxidant (tocopherol), 0.5% preservative (0.4% phenoxyethanol, 0.1% ethylhexylglycerin) and the remainder water;
含茶多酚的协同增效修复组合物面霜的制备方法包括以下步骤：The preparation method of the synergistic repairing composition cream containing tea polyphenols comprises the following steps:
将A相各组分搅拌混合均匀，加热到70 ℃，搅拌15 min。将B相各组分搅拌混合均匀，加热到70 ℃，搅拌溶解完全。将B相加入A相，搅拌混合均匀，维持温度在70 ℃均质10min左右，开始降温。温度降到60 ℃，加入C相各组分，搅拌混合均匀，温度降到45 ℃，加入D相各组分，搅拌混合均匀继续降温到37 ℃制得含茶多酚的协同增效修复组合物面霜。Stir and mix the components of phase A evenly, heat to 70°C, and stir for 15 min. Stir and mix the components of phase B evenly, heat to 70°C, and stir until completely dissolved. Add phase B to phase A, stir and mix evenly, maintain the temperature at 70°C for about 10 minutes, and start cooling. When the temperature drops to 60°C, add the components of phase C, stir and mix evenly, drop the temperature to 45°C, add the components of phase D, stir and mix evenly, and continue to cool to 37°C to obtain a synergistic repair composition cream containing tea polyphenols.
对比例8：Comparative Example 8:
与实施例2相比，对比例8中未添加含茶多酚的协同增效修复组合物，其余条件均与实施例2相同。Compared with Example 2, in Comparative Example 8, no synergistic repair composition containing tea polyphenols was added, and the other conditions were the same as those in Example 2.
测试例2：Test Example 2:
对实施例2和对比例8制得的面霜进行功效评价，测试项目包括：斑贴测试、保湿测试、皮肤修护测试、使用者评价测试；The facial creams prepared in Example 2 and Comparative Example 8 were evaluated for their efficacy, and the test items included: patch test, moisturizing test, skin repair test, and user evaluation test;
斑贴测试步骤：邀请受试者共30人，年龄在20岁至30岁之间，均为女性，随机分为2组，每组15人，做贴斑测试。分别取面霜A、面霜B约0.025~0.03 g样品置于斑试器内，分别用医用胶带贴于受试者的前臂曲侧，持续24 h，分别于移除受试物的0.5 h，24 h和48 h观察皮肤反应，按照《化妆品卫生规范》中皮肤反应记录结果，标准如表1所示；Patch test steps: Invite 30 subjects, aged between 20 and 30 years old, all female, and randomly divide them into 2 groups, 15 in each group, for patch test. Take about 0.025-0.03 g of cream A and cream B respectively and place them in the patch tester, and stick them on the curved side of the forearm of the subjects with medical tape for 24 hours. Observe the skin reaction 0.5 hours, 24 hours and 48 hours after removing the test substances, and record the skin reaction results according to the "Cosmetic Hygiene Standards". The standards are shown in Table 1;
表1 斑贴测试反应标准Table 1 Patch test reaction criteria
斑贴测试结果显示全部受试者均为阴性反应，这表明实施例2和对比例8的面霜安全温和无刺激，符合《化妆品卫生规范》的标准。The patch test results showed that all subjects had negative reactions, which indicates that the creams of Example 2 and Comparative Example 8 are safe, mild and non-irritating, and meet the standards of the "Cosmetic Hygiene Standards".
保湿测试步骤：选取20岁至50岁的35名女性作为受试者，将实施例2和对比例8制得的面霜分别涂抹于受试者侧臂，测试涂抹1 h、涂抹2 h和涂抹4 h的皮肤含水率；测试结果见图4；Moisturizing test steps: 35 women aged 20 to 50 were selected as subjects, and the creams prepared in Example 2 and Comparative Example 8 were applied to the side arms of the subjects, respectively, and the skin moisture content after 1 hour, 2 hours and 4 hours of application was tested; the test results are shown in Figure 4;
如图4所示，实施例2与对比例8相比，在涂抹1 h后受试者皮肤含水率平均提高了8.89 ％，在涂抹2 h后受试者皮肤含水率平均提高了9.18 ％，在涂抹4 h后受试者皮肤含水率平均提高了7.85 ％，上述结果表明，本发明提供的含茶多酚的协同增效修复组合物面霜具有优异的皮肤保湿功效。As shown in Figure 4, compared with Comparative Example 8, the skin moisture content of the subjects in Example 2 increased by an average of 8.89% after 1 hour of application, the skin moisture content of the subjects increased by an average of 9.18% after 2 hours of application, and the skin moisture content of the subjects increased by an average of 7.85% after 4 hours of application. The above results show that the synergistic repair composition cream containing tea polyphenols provided by the present invention has excellent skin moisturizing effect.
皮肤修护测试：受试者选取68名年龄在25岁至55岁的女性，随机分为2组，每组34人，以组为单位分别对应测试实施例2和对比例8制得的面霜，将实施例2和对比例8制得的面霜涂抹于受试者的面部，使用28天，每天早晚各一次，测试受试者涂抹样品前、使用样品14天和使用样品28天后的皮肤经表皮水分流失TEWL值，测试结果见图5；Skin repair test: 68 women aged 25 to 55 were selected as subjects and randomly divided into 2 groups, each with 34 people. The groups were respectively tested with the creams prepared in Example 2 and Comparative Example 8. The creams prepared in Example 2 and Comparative Example 8 were applied to the faces of the subjects for 28 days, once in the morning and once in the evening. The TEWL values of the skin of the subjects were tested before applying the samples, 14 days after using the samples, and 28 days after using the samples. The test results are shown in Figure 5;
如图5所示，使用面霜14天后和28天后实施例2和对比例8均可显著减少经表皮水分流失量TWEL值，且实施例2的减少量显著高于对比例8，上述结果表明添加含茶多酚的协同增效修复组合物的面霜能够显著减少经表皮水分流失量TWEL值，能够显著增强面霜的修复皮肤效果。As shown in Figure 5, both Example 2 and Comparative Example 8 can significantly reduce the transepidermal water loss TWEL value after using the cream for 14 days and 28 days, and the reduction amount of Example 2 is significantly higher than that of Comparative Example 8. The above results show that the cream containing the synergistic repair composition containing tea polyphenols can significantly reduce the transepidermal water loss TWEL value and can significantly enhance the skin repair effect of the cream.
使用者评价测试步骤：选取68名年龄在25岁至55岁的女性志愿者，随机分为2组，每组34人，以组为单位分别对应测试实施例2和对比例8的面霜；将实施例2和对比例8制得的面霜涂抹于面部，每日两次，连续使用4W，通过志愿者主观认知填写调查问卷，4W后收集问卷；调查问卷项目包括的产品温和性、皮肤舒缓性、改善皮肤粗糙、细致毛孔和柔嫩光滑肌肤，对收集后的调查问卷的平均分进行统计，测试结果见图6；User evaluation test steps: 68 female volunteers aged 25 to 55 were selected and randomly divided into 2 groups, each with 34 people, and the groups were used to test the creams of Example 2 and Comparative Example 8 respectively; the creams prepared in Example 2 and Comparative Example 8 were applied to the face twice a day for 4 weeks, and the volunteers filled in the questionnaire through subjective cognition, and the questionnaires were collected after 4 weeks; the questionnaire items included product mildness, skin soothing, improvement of skin roughness, fine pores and soft and smooth skin, and the average scores of the collected questionnaires were statistically analyzed, and the test results are shown in Figure 6;
如图6所示，实施例2和对比例8的产品温和性均为100分，实施例2皮肤舒缓评分为90分，改善皮肤粗糙的评分为68分，柔嫩光滑皮肤的评分为75分，对比例8皮肤舒缓评分为68分，改善皮肤粗糙的评分为65分，柔嫩光滑皮肤的评分为64分，上述结果表明，本发明添加含茶多酚的协同增效修复组合物的化妆品在实际应用中安全温和无刺激，同时还具有舒缓皮肤不适、改善皮肤粗糙和柔嫩肌肤的技果。As shown in Figure 6, the mildness of the products of Example 2 and Comparative Example 8 are both 100 points, the skin soothing score of Example 2 is 90 points, the score for improving rough skin is 68 points, and the score for tender and smooth skin is 75 points, and the skin soothing score of Comparative Example 8 is 68 points, the score for improving rough skin is 65 points, and the score for tender and smooth skin is 64 points. The above results show that the cosmetics of the present invention adding the synergistic repair composition containing tea polyphenols are safe, mild and non-irritating in actual applications, and also have the effects of soothing skin discomfort, improving rough skin and softening skin.
以上所述，仅是本发明的较佳实施例，并非对本发明作任何限制，凡是根据本发明技术实质对以上实施例所作的任何简单修改、变更以及等效变换，均仍属于本发明技术方案的保护范围。The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent transformation made to the above embodiment based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.
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| CN119818418A (en)* | 2025-03-13 | 2025-04-15 | 花安堂生物科技集团有限公司 | Mitochondrial energy improving composition based on plant stem cells, application and cosmetics |
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102846514A (en)* | 2012-09-12 | 2013-01-02 | 杭州小青生物科技有限公司 | Tea-polyphenol-containing hydrogel used in after-sun reparation, and preparation method thereof |
| CN111557882A (en)* | 2020-07-02 | 2020-08-21 | 吉林省强参生物技术有限公司 | Composition and application thereof in preparing anti-aging skin care product |
| CN113332196A (en)* | 2021-05-31 | 2021-09-03 | 美卓(杭州)医疗科技有限公司 | Green tea polyphenol repair gel for deep skin repair and preparation method thereof |
| CN114748366A (en)* | 2022-04-08 | 2022-07-15 | 上海大学 | Anti-cracking moisturizing body care cream and preparation method and application thereof |
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102846514A (en)* | 2012-09-12 | 2013-01-02 | 杭州小青生物科技有限公司 | Tea-polyphenol-containing hydrogel used in after-sun reparation, and preparation method thereof |
| CN111557882A (en)* | 2020-07-02 | 2020-08-21 | 吉林省强参生物技术有限公司 | Composition and application thereof in preparing anti-aging skin care product |
| CN113332196A (en)* | 2021-05-31 | 2021-09-03 | 美卓(杭州)医疗科技有限公司 | Green tea polyphenol repair gel for deep skin repair and preparation method thereof |
| CN114748366A (en)* | 2022-04-08 | 2022-07-15 | 上海大学 | Anti-cracking moisturizing body care cream and preparation method and application thereof |
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| CN119818418A (en)* | 2025-03-13 | 2025-04-15 | 花安堂生物科技集团有限公司 | Mitochondrial energy improving composition based on plant stem cells, application and cosmetics |
| CN119818418B (en)* | 2025-03-13 | 2025-07-15 | 花安堂生物科技集团有限公司 | Mitochondrial energy improving composition based on plant stem cells, application and cosmetics |
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