技术领域Technical Field
本发明属于医疗器械领域，具体涉及一种用于快速分离、收集、低温储存供体血液的多功能血液回收装置及其使用方法。The present invention belongs to the field of medical devices, and in particular relates to a multifunctional blood recovery device for rapid separation, collection and low-temperature storage of donor blood and a method of using the same.
背景技术Background technique
血液作为一种稀缺的公共资源，在挽救患者生命、保障临床疾病的诊疗上起着重要的作用。血液制剂保存期较短且常出现季节性和结构性的供应不足。As a scarce public resource, blood plays an important role in saving patients' lives and ensuring the diagnosis and treatment of clinical diseases. Blood products have a short shelf life and often suffer from seasonal and structural supply shortages.
器官移植是挽救终末器官疾病患者的唯一有效途径，该类患者常常有合并性凝血功能差，血小板低下；并且手器官移植手术较为复杂，术中出现的失血也较多，因此经常需要充足血液储备，否则不仅手术将无法安全进行，甚至患者的生命都会面临严重威胁。Organ transplantation is the only effective way to save patients with end-organ diseases. Such patients often have combined poor coagulation function and low platelet count. In addition, organ transplantation surgery is relatively complicated and involves a lot of blood loss during the operation. Therefore, sufficient blood reserves are often required. Otherwise, not only will the operation be unable to be performed safely, but the patient's life may also be seriously threatened.
尽管静态冷保存(SCS)目前仍是器官保存最常用的技术。然而，由于当下供体器官短缺形势严峻，世界各国都不得不采取措施扩大供体来源，采纳边缘供体(ECD)，包括DCD、老年供者、肥胖供者等，该类边缘供体使移植物功能延迟恢复(DGF)和原发性移植物无功能(PNF)发生率显著增加,在这方面，SCS技术已经无法满足临床需要。近年来，常温机械灌注因可以在常温下给器官供血供氧，提供接近人体生理状态的养护环境，相比较SCS,在减少器官缺血再灌注损伤、扩大供体来源和提高移植预后都有显著优势。然而，充足的洗涤红细胞供应(一般每个器官体外常温灌注需要8～10U)仍然是常温机械灌注开展的重要制约条件，因此，为了避免器官浪费和提高移植预后，寻找出一种能够缓解血液供应的途径迫在眉睫。Although static cold storage (SCS) is still the most commonly used technology for organ preservation. However, due to the current severe shortage of donor organs, countries around the world have to take measures to expand the source of donors and adopt marginal donors (ECD), including DCD, elderly donors, obese donors, etc. Such marginal donors significantly increase the incidence of delayed graft function (DGF) and primary graft nonfunction (PNF). In this regard, SCS technology can no longer meet clinical needs. In recent years, normothermic mechanical perfusion can supply blood and oxygen to organs at room temperature and provide a maintenance environment close to the physiological state of the human body. Compared with SCS, it has significant advantages in reducing organ ischemia-reperfusion injury, expanding donor sources, and improving transplantation prognosis. However, sufficient supply of washed red blood cells (generally 8 to 10 U of each organ is required for in vitro normothermic perfusion) is still an important constraint for the development of normothermic mechanical perfusion. Therefore, in order to avoid organ waste and improve transplantation prognosis, it is urgent to find a way to relieve blood supply.
目前，由于捐献器官获取时，通常只是获取心、肺、肝肾等实体器官，而供体血液目前由于缺乏一套规范且有效的回收技术，导致了宝贵的供体血液资源的浪费。此外，按照正常体重的计算，一个成人体内血液总量将达到4000～5000ml左右，因此，如果建立一套完整的捐献供体血液回收、分离及保存体系，将其应用在常温机械灌注体外器官保存或手术备血，可极大地缓解当前用血紧张，并实现真正意义上的变废为宝。At present, when donating organs, usually only solid organs such as the heart, lungs, liver and kidneys are obtained, and the lack of a standardized and effective recovery technology for donor blood has led to a waste of precious donor blood resources. In addition, according to the calculation of normal weight, the total amount of blood in an adult will reach about 4000-5000ml. Therefore, if a complete system for the recovery, separation and preservation of donated donor blood is established and applied to the preservation of organs in vitro or blood preparation for surgery at room temperature mechanical perfusion, it can greatly alleviate the current blood shortage and realize the true meaning of turning waste into treasure.
发明内容Summary of the invention
基于此，本发明提供一种用于快速分离、收集、低温储存供体血液的多功能血液回收装置及其使用方法。Based on this, the present invention provides a multifunctional blood recovery device for rapid separation, collection, and cryogenic storage of donor blood and a method for using the same.
为了实现上述目的，本发明采用如下技术方案：In order to achieve the above object, the present invention adopts the following technical solution:
一种多功能血液回收装置，所述多功能血液回收装置包括：A multifunctional blood recovery device, comprising:
下腔静脉血流采集端，包括高通量血液采集管；An inferior vena cava blood flow collection end, including a high-throughput blood collection tube;
血液有效成分分离系统，包括储血罐、五通管、生理盐水清洗装置、分离泵、血小板储罐、血浆收集袋；其中，所述生理盐水清洗装置包括生理盐水储罐和冲洗管道；A blood active component separation system, comprising a blood storage tank, a five-way pipe, a physiological saline cleaning device, a separation pump, a platelet storage tank, and a plasma collection bag; wherein the physiological saline cleaning device comprises a physiological saline storage tank and a flushing pipeline;
供体血液净化系统，包括直线加速器、血液透析装置和白细胞过滤装置；Donor blood purification systems, including linear accelerators, hemodialysis devices, and leukocyte filtration devices;
血液有效成分收集系统，包括一氧化氮输送装置、多个血液有效成分冷藏箱，所述血液有效成分冷藏箱分别通过红细胞回收管道和血小板回收管道与所述血液有效成分分离系统连通；A blood active component collection system, comprising a nitric oxide delivery device and a plurality of blood active component refrigerators, wherein the blood active component refrigerators are connected to the blood active component separation system through a red blood cell recovery pipeline and a platelet recovery pipeline respectively;
冷却系统；cooling system;
血液有效成分快速复苏装置；Blood active component rapid resuscitation device;
所述下腔静脉血流采集端通过第一管道与所述储血罐连通，所述储血罐通过第二管道与所述五通管的第一端连通，所述五通管的第二端与所述生理盐水清洗装置的所述冲洗管道连通，所述五通管的第三端与所述红细胞回收管道连通，所述五通管的第四端通过二通盖与所述分离泵连通，所述五通管的第五端通过回返管道与血小板储罐连通。The inferior vena cava blood flow collection end is connected to the blood storage tank through a first pipe, the blood storage tank is connected to the first end of the five-way pipe through a second pipe, the second end of the five-way pipe is connected to the flushing pipe of the physiological saline cleaning device, the third end of the five-way pipe is connected to the red blood cell recovery pipe, the fourth end of the five-way pipe is connected to the separation pump through a two-way cover, and the fifth end of the five-way pipe is connected to the platelet storage tank through a return pipe.
进一步地，所述多功能血液回收装置还设置有抗凝系统，所述抗凝系统设置有抗凝剂储罐，所述抗凝剂储罐通过抗凝剂管道与所述第一管道连通，所述抗凝剂管道连接在所述下腔静脉血流采集端和所述储血罐之间。Furthermore, the multifunctional blood recovery device is also provided with an anticoagulation system, and the anticoagulation system is provided with an anticoagulant storage tank, and the anticoagulant storage tank is connected to the first pipeline through an anticoagulant pipeline, and the anticoagulant pipeline is connected between the inferior vena cava blood flow collection end and the blood storage tank.
进一步地，在所述血液有效成分分离系统中，储血罐、五通阀分离泵、血小板储罐、血浆收集袋依次连通，所述二通盖安装在所述分离泵的泵头上，与所述分离泵的离心杯连通，所述二通盖的第一端与所述五通管的第四端连通，所述二通盖的第二端通过第三管道与所述血小板储罐连通，所述血浆收集袋通过血浆收集管道与所述血小板储罐连通。Furthermore, in the blood effective component separation system, the blood storage tank, the five-way valve separation pump, the platelet storage tank, and the plasma collection bag are connected in sequence, the two-way cover is installed on the pump head of the separation pump and is connected to the centrifugal cup of the separation pump, the first end of the two-way cover is connected to the fourth end of the five-way tube, the second end of the two-way cover is connected to the platelet storage tank through the third pipeline, and the plasma collection bag is connected to the platelet storage tank through the plasma collection pipeline.
进一步地，在所述供体血液净化系统中，所述直线加速器设置在所述储血罐的一侧并使射线输出端正对所述储血罐，能够杀灭供体血液中残留的游离肿瘤细胞；所述透析装置和所述白细胞过滤装置设置在所述第二管道上，其中，所述血液透析装置设置在所述白细胞过滤装置的上方。Furthermore, in the donor blood purification system, the linear accelerator is arranged on one side of the blood storage tank and the ray output end is facing the blood storage tank, which can kill the free tumor cells remaining in the donor blood; the dialysis device and the leukocyte filter device are arranged on the second pipeline, wherein the blood dialysis device is arranged above the leukocyte filter device.
进一步地，在所述血液有效成分收集系统中，所述红细胞回收管道的第一端与所述五通管的第三端连通，所述红细胞回收管道的第二端设置有多条回收支管，多条所述回收支管分别与多个所述血液有效成分冷藏箱一一对应连通；所述血小板回收管道的第一端与所述血小板储罐连通，所述血小板回收管道的第二端设置有多条回收支管，多条所述回收支管分别与多个所述血液有效成分冷藏箱一一对应连通。Furthermore, in the blood effective component collection system, the first end of the red blood cell recovery pipeline is connected to the third end of the five-way pipe, and the second end of the red blood cell recovery pipeline is provided with multiple recovery branches, and the multiple recovery branches are respectively connected to the multiple blood effective component refrigerators in a one-to-one correspondence; the first end of the platelet recovery pipeline is connected to the platelet storage tank, and the second end of the platelet recovery pipeline is provided with multiple recovery branches, and the multiple recovery branches are respectively connected to the multiple blood effective component refrigerators in a one-to-one correspondence.
进一步地，所述一氧化氮输送装置包括一氧化氮储罐，所述一氧化氮储罐在开口处设置有气体节流阀，所述气体节流阀通过气密接头与所述气体输送管道的第一端连通，所述气体输送管道的第二端通过气体阀门与所述红细胞回收管道连通。Furthermore, the nitric oxide delivery device includes a nitric oxide storage tank, which is provided with a gas throttle valve at the opening, the gas throttle valve is connected to the first end of the gas delivery pipeline through an airtight joint, and the second end of the gas delivery pipeline is connected to the red blood cell recovery pipeline through a gas valve.
进一步地，所述冷却系统包括压缩机、冷却风扇和冷凝管道，所述压缩机与所述冷却风扇设置在多个所述血液有效成分冷藏箱附近区域；多个所述冷凝管道一一对应设置在多个所述血液有效成分冷藏箱外围。Furthermore, the cooling system includes a compressor, a cooling fan and a condensing duct, the compressor and the cooling fan are arranged in the vicinity of the multiple blood active component refrigerators; the multiple condensing ducts are arranged one-to-one at the periphery of the multiple blood active component refrigerators.
进一步地，所述血液有效成分快速复苏装置包括体外膜肺装置，多个所述血液有效成分冷藏箱通过上总输出管道与所述体外膜肺装置连通，所述体外膜肺装置内设置有水浴流通管道、有效成分流通管道，所述水浴流通管道与有效成分流通管道均呈螺旋状且并列设置，流过水浴流通管道的温水与有效成分流通管道中的血液有效成分进行热交换，氧气经输氧管道进入血液有效成分流通管道并与血液有效成分混合。Furthermore, the blood effective component rapid resuscitation device includes an extracorporeal membrane lung device, and a plurality of the blood effective component refrigerators are connected to the extracorporeal membrane lung device through an upper main output pipe. A water bath circulation pipe and an effective component circulation pipe are arranged in the extracorporeal membrane lung device. The water bath circulation pipe and the effective component circulation pipe are both spiral and arranged in parallel. The warm water flowing through the water bath circulation pipe exchanges heat with the blood effective components in the effective component circulation pipe, and oxygen enters the blood effective component circulation pipe through the oxygen supply pipe and mixes with the blood effective components.
进一步地，所述多功能血液回收装置还包括有离心泵、节流阀、负压吸引源、快速接头，至少部分所述管道设置有所述离心泵和所述节流阀，所述储血罐和所述血小板储罐分别设置有与其罐腔连通的所述负压吸引源，在管道与管道、所述五通阀、所述二通盖、储血罐、血小板储罐以及血液有效成分冷藏箱之间的连接处均设置有快速接头。Furthermore, the multifunctional blood recovery device also includes a centrifugal pump, a throttle valve, a negative pressure suction source, and a quick connector. At least part of the pipeline is provided with the centrifugal pump and the throttle valve, the blood storage tank and the platelet storage tank are respectively provided with the negative pressure suction source connected to their tank cavities, and quick connectors are provided at the connections between the pipelines, the five-way valve, the two-way cover, the blood storage tank, the platelet storage tank and the blood active component refrigerator.
进一步地，还包括一种用于所述多功能血液回收装置的使用方法，包括以下步骤：Furthermore, a method for using the multifunctional blood recovery device is also provided, comprising the following steps:
1)通过高通量血液采集管进行血液采集，连通抗凝系统；1) Collect blood through a high-throughput blood collection tube and connect it to the anticoagulation system;
2)开启供体血液净化系统，进行血液净化；2) Turn on the donor blood purification system to purify the blood;
3)当离心杯中的供体血液达到一定阈值时，暂时关闭第二管道，启动分离泵，进行第一次血液有效成分的分离；3) When the donor blood in the centrifuge cup reaches a certain threshold, the second pipeline is temporarily closed, and the separation pump is started to separate the effective components of the blood for the first time;
4)控制生理盐水冲洗液经冲洗管道流入五通管，血浆、血小板成分与生理盐水的混合液一同经第三管道流入血小板储罐；4) Control the physiological saline flushing solution to flow into the five-way pipe through the flushing pipe, and the mixture of plasma, platelet components and physiological saline flows into the platelet storage tank through the third pipe;
5)开启红细胞回收管道阀门、一氧化氮储罐阀门、气体输送管道阀门，并调整气体输送管道中的一氧化氮的流量，控制血液有效成分冷藏箱的温度，对其中的红细胞成分的低温冷藏；5) Open the valve of the red blood cell recovery pipeline, the valve of the nitric oxide storage tank, and the valve of the gas delivery pipeline, and adjust the flow of nitric oxide in the gas delivery pipeline, control the temperature of the blood active component refrigerator, and refrigerate the red blood cell components therein;
6)当离心杯中的红细胞成分完全流出后，控制血小板与血浆成分经回返管道再次流入五通管，经二通盖流入离心杯，进行第二次血液有效成分的分离；6) After the red blood cell components in the centrifuge cup have completely flowed out, the platelets and plasma components are controlled to flow into the five-way tube again through the return pipe, and then flow into the centrifuge cup through the two-way cover to separate the effective components of the blood for the second time;
7)控制生理盐水冲洗液经冲洗管道再次流入五通管，血浆与生理盐水的混合液一同经第三管道流入血小板储罐；7) Control the saline flushing solution to flow into the five-way pipe again through the flushing pipe, and the mixture of plasma and saline flows into the platelet storage tank through the third pipe;
8)开启血小板回收管道阀门，控制血液有效成分冷藏箱的温度，对其中的血小板成分的低温冷藏；8) Open the valve of the platelet recovery pipeline, control the temperature of the blood active component refrigerator, and refrigerate the platelet components therein;
9)当离心杯中的血小板成分完全流出后，重新打开第二管道，并重复步骤3)-步骤8)；9) When the platelet components in the centrifuge cup have completely flowed out, reopen the second channel and repeat steps 3) to 8);
10)红细胞成分进入体外膜肺装置，控制温水流入体外膜肺装置中的水浴流通管道，温水与血液有效成分流通管道中的红细胞成分发生热交换，实现低温冻存红细胞的复苏；10) The red blood cell components enter the extracorporeal membrane lung device, and the warm water is controlled to flow into the water bath circulation pipe in the extracorporeal membrane lung device. The warm water and the red blood cell components in the blood effective component circulation pipe undergo heat exchange, thereby realizing the recovery of low-temperature frozen red blood cells;
11)红细胞成分经下总输出管道输出至外部待用。11) The red blood cell components are output to the outside through the lower main output pipeline for standby use.
与现有技术相比，本发明具有如下特点和有益效果：Compared with the prior art, the present invention has the following characteristics and beneficial effects:
通过在供体血液的插管取血手术中使用带有微孔的高通量血液采集管进行下腔静脉插管取血，相较于传统的插管取血方式，大幅增加了下腔静脉血液的有效回收面积，增加了单位时间内的血液回收量，实现了供体血液的快速、充分的采集，保证了供体血液采集的效率与供体血液的生理活性；By using a high-throughput blood collection tube with micropores to draw blood from the inferior vena cava during the cannulation of donor blood, the effective recovery area of the inferior vena cava blood is greatly increased compared to the traditional cannulation method, and the blood recovery volume per unit time is increased, thereby achieving rapid and sufficient collection of donor blood, and ensuring the efficiency of donor blood collection and the physiological activity of donor blood;
设置由直线加速器、血液透析装置、白细胞滤器组成的血液净化系统，相较于传统的供体血液采集，实现在血液采集的动态过程中同步进行对供体血液中肿瘤细胞、病毒、免疫细胞、代谢废物等成分的有效清除，无需在血液收集完成后另做处理，而直接可以用于临床使用，大幅减小了供体血液的处理成本，扩大了可用于收集血液的血液供体群体，简化了供体血液再利用的流程；The blood purification system consisting of a linear accelerator, a hemodialysis device, and a leukocyte filter is set up. Compared with the traditional donor blood collection, it can realize the effective removal of tumor cells, viruses, immune cells, metabolic waste and other components in the donor blood in the dynamic process of blood collection. There is no need to do additional processing after the blood collection is completed, and it can be directly used for clinical use, which greatly reduces the processing cost of donor blood, expands the blood donor group that can be used for blood collection, and simplifies the process of donor blood reuse;
通过带有回返管道的血液有效成分分离系统与血液有效成分收集系统，相较于传统的供体血液收集方式，实现在血液采集的动态过程中同步进行红细胞成分、血小板成分、血浆成分的独立且连续的分离回收、分装、保存，无需在完成供体血液的收集之后再另外人工分离各个血液组成成分，从而实现了某个或某几个血液组成成分在临床上的独立使用，大幅减小了供体血液的处理成本，精细化了供体血液再利用的方式；Compared with the traditional donor blood collection method, the blood effective component separation system and blood effective component collection system with return pipes can realize the independent and continuous separation, recovery, packaging and storage of red blood cell components, platelet components and plasma components in the dynamic process of blood collection, without the need to manually separate the blood components after the collection of donor blood is completed, thereby realizing the independent use of one or several blood components in clinical practice, greatly reducing the processing cost of donor blood, and refining the way of donor blood reuse;
通过带有独立制冷单元的可自定义使用数量的血液有效成分冷藏箱，相较于传统的供体血液收集过程，实现在血液采集的动态过程中同步进行血液组成成分的低温冻存，且可以实现对任意一个或若干个冷藏箱的独立温度控制，从而灵活实现不同血液组成成分的多种不同温度的密封保存；Compared with the traditional donor blood collection process, the blood components can be cryopreserved synchronously during the dynamic process of blood collection through the blood active component refrigerator with independent refrigeration unit and the independent temperature control of any one or several refrigerators can be realized, so as to flexibly realize the sealed storage of different blood components at different temperatures;
通过针对红细胞成分收集与储存的一氧化氮输送系统，相较于传统的供体血液收集与储存，实现对红细胞成分的实时一氧化氮补充，以此在较长时间的低温储存中改善红细胞因为缺乏一氧化氮而导致的变形与携氧能力下降，使红细胞再次输出用于临床使用时仍然具备较理想的生理活性，由此实现红细胞输血疗效的进一步提升；Compared with the traditional collection and storage of donor blood, the nitric oxide delivery system for the collection and storage of red blood cell components can achieve real-time nitric oxide supplementation of red blood cell components, thereby improving the deformation and decreased oxygen-carrying capacity of red blood cells caused by lack of nitric oxide during long-term low-temperature storage, so that the red blood cells still have ideal physiological activity when they are re-transfused for clinical use, thereby further improving the efficacy of red blood cell transfusion;
设置体外膜肺装置，相较于传统的供体血液的临床再使用，使经长时间低温冻存的血液有效成分能够实现快速复苏，实现红细胞携氧量的快速恢复，极大简化了血液有效成分临床再使用的流程，在用于抢救急性大出血病人等特殊临床情景时可发挥重要作用。Compared with the traditional clinical reuse of donor blood, the installation of an extracorporeal membrane oxygenation device allows the rapid resuscitation of the effective components of blood that have been frozen at low temperatures for a long time, and the rapid recovery of the oxygen carrying capacity of red blood cells. This greatly simplifies the process of clinical reuse of effective blood components and can play an important role in special clinical scenarios such as rescuing patients with acute massive bleeding.
附图说明BRIEF DESCRIPTION OF THE DRAWINGS
为了更清楚地说明本发明实施方式的技术方案，下面将对实施方式中所需要使用的附图作简单地介绍，应当理解，以下附图仅示出了本发明的某些实施例，因此不应被看作是对范围的限定，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他相关的附图。In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.
图1为本专利一实施例下快速收集、分离、低温储存供体血液的多功能血液回收装置的结构示意图。FIG1 is a schematic diagram of the structure of a multifunctional blood recovery device for rapidly collecting, separating, and cryogenically storing donor blood according to an embodiment of the present patent.
图中：A-血液供体；B-供体心脏；C-供体下腔静脉；In the figure: A-blood donor; B-donor heart; C-donor inferior vena cava;
01-高通量血液采集管；01a-快速接头；02-抗凝剂储罐；03-抗凝剂节流阀；01-high throughput blood collection tube; 01a-quick connector; 02-anticoagulant storage tank; 03-anticoagulant throttle valve;
04-第一离心泵；05-抗凝剂管道；05a-第一阀门；06-第一管道；04-first centrifugal pump; 05-anticoagulant pipeline; 05a-first valve; 06-first pipeline;
06a-第一负压吸引源；07-储血罐；07a-快速接头；08-第二管道；08a-快速接头；09-第二离心泵；10-生理盐水储罐；10a-快速接头；11-生理盐水节流阀；06a-first negative pressure suction source; 07-blood storage tank; 07a-quick connector; 08-second pipeline; 08a-quick connector; 09-second centrifugal pump; 10-normal saline storage tank; 10a-quick connector; 11-normal saline throttle valve;
12-冲洗管道；12a-快速接头；13-第三离心泵；14-五通管；14a、14b-快速接头；15-二通盖；15a、15b-快速接头；12-flushing pipe; 12a-quick connector; 13-third centrifugal pump; 14-five-way pipe; 14a, 14b-quick connector; 15-two-way cover; 15a, 15b-quick connector;
16-分离泵泵头；17-分离泵离心杯、离心井(未画出)；18-第四离心泵；19-红细胞回收管道；16-separation pump head; 17-separation pump centrifugal cup, centrifugal well (not shown); 18-fourth centrifugal pump; 19-red blood cell recovery pipeline;
19a1～19an-第一红细回收管道阀门～第n红细胞回收管道阀门；19a1～19an-the first red blood cell recovery pipeline valve~the nth red blood cell recovery pipeline valve;
19b1～19bn-第一红细胞回收支管～第n红细胞回收支管；19b1~19bn-the first red blood cell recovery branch tube~the nth red blood cell recovery branch tube;
19c1～19cn-第一红细胞回收管道快速接头～第n红细胞回收管道快速接头；19c1～19cn-the first red blood cell recovery pipeline quick connector~the nth red blood cell recovery pipeline quick connector;
20-第三管道；20-第三管道；20a-第二负压吸引源；21-血小板储罐；20-third pipeline; 20-third pipeline; 20a-second negative pressure suction source; 21-platelet storage tank;
21a、21b、21c-快速接头；22-回返管道；22a-快速接头；23-第五离心泵；21a, 21b, 21c-quick connectors; 22-return pipe; 22a-quick connector; 23-fifth centrifugal pump;
24-血浆收集管道；24a-快速接头；25-血浆收集袋；26-第六离心泵；24-plasma collection pipeline; 24a-quick connector; 25-plasma collection bag; 26-sixth centrifugal pump;
27-血小板回收管道；27-Platelet recovery tube;
27a1～27an-第一血小板回收管道阀门～第n血小板回收管道阀门；27a1~27an-the first platelet recovery pipeline valve~the nth platelet recovery pipeline valve;
27b1～27bn-第一血小板回收支管～第n血小板回收支管；27b1~27bn-the first platelet recovery branch tube~the nth platelet recovery branch tube;
27c1～27cn-第一血小板回收管道快速接头～第n血小板回收管道快速接头；27c1~27cn-the first platelet recovery pipeline quick connector~the nth platelet recovery pipeline quick connector;
28-1～28-n-第一血液有效成分冷藏箱～第n血液有效成分冷藏箱；28-1 to 28-n - the first blood active component refrigerator to the nth blood active component refrigerator;
28a1～28an-第一冷藏箱快速接头～第n冷藏箱快速接头；28a1～28an-the first refrigerated container quick connector~the nth refrigerated container quick connector;
29-1～29-n-第一输出管道～第n输出管道；29b-上总输出管道；29-1 to 29-n - first output pipeline to nth output pipeline; 29b - upper main output pipeline;
29a1～29an-第一输出阀门～第n输出阀门；29c-快速接头；30-一氧化氮储罐；29a1~29an-first output valve~nth output valve; 29c-quick connector; 30-nitric oxide storage tank;
30a-一氧化氮储罐阀门；30b-气密接头；31-气体输送管道；30a-nitric oxide storage tank valve; 30b-airtight joint; 31-gas delivery pipeline;
31a-气体输送管道阀门；32-气泵；33-体外膜肺；33a、33b-快速接头；31a-gas delivery pipeline valve; 32-air pump; 33-extracorporeal membrane lung; 33a, 33b-quick connector;
34-温水输入管道；34a-快速接头；35-输氧管道；35a-快速接头；34-warm water input pipe; 34a-quick connector; 35-oxygen supply pipe; 35a-quick connector;
36-冷水输出管道；37-下总输出管道；36-cold water output pipeline; 37-lower main output pipeline;
38-第七离心泵；39-压缩机；40-冷却风扇；38-seventh centrifugal pump; 39-compressor; 40-cooling fan;
40a1～40an-第一冷凝管道阀门～第n冷凝管道阀门；40a1~40an-the first condensation pipeline valve~the nth condensation pipeline valve;
41-1～41-n-第一冷凝管道～第n冷凝管道；41-1 to 41-n - first condensation pipeline to nth condensation pipeline;
42-1～42-n-第一冷媒节流阀～第n冷媒节流阀；43-直线加速器；44-血液透析装置；42-1 to 42-n: first refrigerant throttle valve to nth refrigerant throttle valve; 43: linear accelerator; 44: hemodialysis device;
45-白细胞过滤装置。45-Leukocyte filtration device.
具体实施方式Detailed ways
在本发明的描述中，需要理解的是，涉及到方位描述，例如上、下、左、右等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本发明和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。In the description of the present invention, it should be understood that descriptions involving orientation, such as up, down, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
在本发明的描述中，若干的含义是一个以上，多个的含义是两个以上。如果有描述到第一、第二只是用于区分技术特征为目的，而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量或者隐含指明所指示的技术特征的先后关系。In the description of the present invention, "several" means more than one, and "more" means more than two. If there is a description of "first" or "second", it is only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.
本发明的描述中，除非另有明确的限定，设置、连接、连通、导入等词语应做广义理解，所属技术领域技术人员可以结合技术方案的具体内容合理确定上述词语在本发明中的具体含义。In the description of the present invention, unless otherwise clearly defined, words such as setting, connecting, communicating, and introducing should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above words in the present invention in combination with the specific content of the technical solution.
本发明的描述中，参考术语“一个实施例”、“一些实施例”等的描述意指结合该实施例描述的具体特征、结构、材料或者特点包含于本发明的至少一个实施例中。在本说明书中，对上述术语的示意性表述不一定指的是相同的实施例。而且，描述的具体特征、结构、材料或者特点可以在任何的一个或多个实施例中以合适的方式结合。In the description of the present invention, reference to the terms "one embodiment", "some embodiments", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment are included in at least one embodiment of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment. Moreover, the specific features, structures, materials or characteristics described can be combined in any suitable manner in one or more embodiments.
为使本发明实施方式的目的、技术方案和优点更加清楚，下面将结合本发明实施方式中的附图，对本发明实施方式中的技术方案进行清楚、完整地描述，显然，所描述的实施方式是本发明一部分实施方式，而不是全部的实施方式。基于本发明中的实施方式，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施方式，都属于本发明保护的范围。In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
实施例一Embodiment 1
本实施例提供了一种能够快速分离、收集、低温储存供体血液的多功能血液回收装置，下面结合图1来描述该装置的结构：This embodiment provides a multifunctional blood recovery device capable of quickly separating, collecting, and storing donor blood at low temperature. The structure of the device is described below in conjunction with FIG. 1 :
该装置包括高通量血液采集管01，其头端沿供体的下腔静脉插入至供体的右心房，高通量血液采集管01上设有密集的微孔，这样设计的好处在于大幅提高在供体内的血液有效收集面积，增加供体血液的收集效率，实现供体血液的快速大量获取；高通量血液采集管01通过快速接头01a与第一管道06的第一端连通；第一管道06的第二端与储血罐07相通。The device includes a high-throughput blood collection tube 01, the head end of which is inserted into the right atrium of the donor along the inferior vena cava of the donor. The high-throughput blood collection tube 01 is provided with dense micropores. The advantage of this design is that it greatly increases the effective blood collection area in the donor, increases the collection efficiency of the donor blood, and realizes the rapid and large-scale acquisition of the donor blood; the high-throughput blood collection tube 01 is connected to the first end of the first pipeline 06 through the quick connector 01a; the second end of the first pipeline 06 is connected to the blood storage tank 07.
抗凝剂储罐02通过快速接头02a与抗凝剂管道05的第一端连通；抗凝剂管道05的第二端通过第一阀门05a与第一管道06相接；抗凝剂节流阀03与第一离心泵04均设置在抗凝剂管道05上，其中，抗凝剂节流阀03设置在第一离心泵04的上方。The anticoagulant storage tank 02 is connected to the first end of the anticoagulant pipeline 05 through the quick connector 02a; the second end of the anticoagulant pipeline 05 is connected to the first pipeline 06 through the first valve 05a; the anticoagulant throttle valve 03 and the first centrifugal pump 04 are both arranged on the anticoagulant pipeline 05, wherein the anticoagulant throttle valve 03 is arranged above the first centrifugal pump 04.
储血罐07的罐腔与第一管道06的第二端相通，储血罐07的罐腔亦与第一负压吸引源06a相通；第一负压吸引源06a可用于制造第一管道06内与储血罐07罐腔内部的压力差，使得供体血液在压力差的作用下顺利经第一管道06流入储血罐07的罐腔内；储血罐07通过带有节流阀的快速接头07a与第二管道08的第一端08连通；第二管道08的第二端通过快速接头08a与五通管14的第一端连通。第二离心泵09设置在第二管道08上，当储血罐07中的供体血液储量达到一定体积时，可以人为开启快速接头07a的节流阀，让血液在第二离心泵制造的管道内压力差作用下流入第二管道08，进而进入五通管14。The tank cavity of the blood storage tank 07 is in communication with the second end of the first pipeline 06, and the tank cavity of the blood storage tank 07 is also in communication with the first negative pressure suction source 06a; the first negative pressure suction source 06a can be used to create a pressure difference between the first pipeline 06 and the tank cavity of the blood storage tank 07, so that the donor blood can smoothly flow into the tank cavity of the blood storage tank 07 through the first pipeline 06 under the action of the pressure difference; the blood storage tank 07 is in communication with the first end 08 of the second pipeline 08 through a quick connector 07a with a throttle valve; the second end of the second pipeline 08 is in communication with the first end of the five-way pipe 14 through a quick connector 08a. The second centrifugal pump 09 is arranged on the second pipeline 08. When the donor blood storage in the blood storage tank 07 reaches a certain volume, the throttle valve of the quick connector 07a can be manually opened to allow the blood to flow into the second pipeline 08 under the action of the pressure difference in the pipeline created by the second centrifugal pump, and then enter the five-way pipe 14.
直线加速器43设置在储血罐07的一侧，在血液短时间储存于储血罐07中时，可以人为开启直线加速器43，直线加速器43的射线输出端正对储血罐07，可以通过照射供体血液达到杀灭供体血液中残留的游离肿瘤细胞的目的；血液透析装置44与白细胞过滤装置45均设置在第二管道08上，其中血液透析装置44设置在白细胞过滤装置45的上方。The linear accelerator 43 is arranged on one side of the blood storage tank 07. When the blood is stored in the blood storage tank 07 for a short time, the linear accelerator 43 can be manually turned on. The ray output end of the linear accelerator 43 faces the blood storage tank 07, and the purpose of killing the free tumor cells remaining in the donor blood can be achieved by irradiating the donor blood; the hemodialysis device 44 and the leukocyte filter device 45 are both arranged on the second pipeline 08, wherein the hemodialysis device 44 is arranged above the leukocyte filter device 45.
五通管14的第一端通过带有节流阀的快速接头08a与第二管道06连通，五通管14的第二端通过快速接头12a与冲洗管道12连通，五通管14的第三端通过快速接头14b与红细胞回收管道连通，五通管14的第四端通过快速接头14a与二通盖15连通，五通管14的第五端通过快速接头22a与回返管道连通。The first end of the five-way tube 14 is connected to the second pipeline 06 through a quick connector 08a with a throttle valve, the second end of the five-way tube 14 is connected to the flushing pipeline 12 through a quick connector 12a, the third end of the five-way tube 14 is connected to the red blood cell recovery pipeline through a quick connector 14b, the fourth end of the five-way tube 14 is connected to the two-way cover 15 through a quick connector 14a, and the fifth end of the five-way tube 14 is connected to the return pipeline through a quick connector 22a.
二通盖15的第一端通过快速接头14a与五通管14连通，二通盖15的第二端通过快速接头15a与第三管道20连通，二通盖15安装在分离泵的泵头16上，与分离泵的离心杯17相通；第三管道20的第一端通过快速接头15a与二通盖15的第二端连通，可用于离心过程中以及对离心杯冲洗过程中次要分离成分的导流；血小板储罐21的罐腔与第三管道20相通，血小板储罐21的罐腔亦与第二负压吸引源20a相通，血小板储罐21可用于离心过程中以及对离心杯冲洗过程中次要分离成分的暂时储存；第二负压吸引源20a可用于制造第三管道20内与血小板储罐21罐腔内部的压力差，使得供体血液在压力差的作用下顺利经第三管道20流入血小板储罐21的罐腔内。The first end of the two-way cover 15 is connected to the five-way pipe 14 through a quick connector 14a, and the second end of the two-way cover 15 is connected to the third pipeline 20 through a quick connector 15a. The two-way cover 15 is installed on the pump head 16 of the separation pump and communicates with the centrifugal cup 17 of the separation pump; the first end of the third pipeline 20 is connected to the second end of the two-way cover 15 through the quick connector 15a, and can be used for guiding the secondary separation components during the centrifugation process and the centrifugal cup flushing process; the tank cavity of the platelet storage tank 21 is connected to the third pipeline 20, and the tank cavity of the platelet storage tank 21 is also connected to the second negative pressure suction source 20a, and the platelet storage tank 21 can be used for temporary storage of secondary separation components during the centrifugation process and the centrifugal cup flushing process; the second negative pressure suction source 20a can be used to create a pressure difference between the third pipeline 20 and the tank cavity of the platelet storage tank 21, so that the donor blood flows smoothly into the tank cavity of the platelet storage tank 21 through the third pipeline 20 under the action of the pressure difference.
生理盐水储罐10通过快速接头10a与冲洗管道12的第一端连通，冲洗管道12的第二端通过带有节流阀的快速接头12a与五通管14的第二端连通；第三离心泵13设置在冲洗管道12上，第三离心泵13可以通过制造管内压力差，将生理盐水储罐10中的生理盐水冲洗液经冲洗管道12、五通管14和二通盖15泵入离心杯17，使得第一次离心后离心杯17内待分离的血小板血浆混合液、第二次离心后离心杯17内待分离的残余血浆成分经第三管道20被冲入血小板储罐21。The physiological saline storage tank 10 is connected to the first end of the flushing pipe 12 through a quick connector 10a, and the second end of the flushing pipe 12 is connected to the second end of the five-way pipe 14 through a quick connector 12a with a throttle valve; the third centrifugal pump 13 is arranged on the flushing pipe 12, and the third centrifugal pump 13 can pump the physiological saline flushing liquid in the physiological saline storage tank 10 into the centrifuge cup 17 through the flushing pipe 12, the five-way pipe 14 and the two-way cover 15 by creating a pressure difference in the pipe, so that the platelet-plasma mixture to be separated in the centrifuge cup 17 after the first centrifugation and the residual plasma components to be separated in the centrifuge cup 17 after the second centrifugation are flushed into the platelet storage tank 21 through the third pipe 20.
红细胞回收管道19的第一端通过带有节流阀的快速接头14b与五通管14的第四端连通；红细胞回收管道19的第二端设有多个末端，红细胞回收管道19的第二端通过第一红细胞回收管道阀门19a1与第一红细胞回收支管19b1的第一端连通，第一红细胞回收支管19b1的第二端通过快速接头19c1与第一血液有效成分冷藏箱连通；以此类推，红细胞回收管道19的第n端通过第n红细胞回收管道阀门19an与第n红细胞回收支管19bn的第一端连通，第n红细胞回收支管的第二端通过快速接头19cn与第n血液有效成分冷藏箱连通；第四离心泵18设置在红细胞回收管道19上，第四离心泵18可以通过制造管内压力差，使得第一次离心后留在离心杯17内的红细胞被经二通盖15和五通管14被吸入红细胞回收管道19。The first end of the red blood cell recovery pipeline 19 is connected to the fourth end of the five-way pipe 14 through a quick connector 14b with a throttle valve; the second end of the red blood cell recovery pipeline 19 is provided with a plurality of ends, the second end of the red blood cell recovery pipeline 19 is connected to the first end of the first red blood cell recovery branch pipe 19b1 through the first red blood cell recovery pipeline valve 19a1, and the second end of the first red blood cell recovery branch pipe 19b1 is connected to the first blood active component refrigerator through the quick connector 19c1; by analogy, the nth end of the red blood cell recovery pipeline 19 is connected to the first end of the nth red blood cell recovery branch pipe 19bn through the nth red blood cell recovery pipeline valve 19an, and the second end of the nth red blood cell recovery branch pipe is connected to the nth blood active component refrigerator through the quick connector 19cn; the fourth centrifugal pump 18 is arranged on the red blood cell recovery pipeline 19, and the fourth centrifugal pump 18 can create a pressure difference in the tube so that the red blood cells remaining in the centrifugal cup 17 after the first centrifugation are sucked into the red blood cell recovery pipeline 19 through the two-way cover 15 and the five-way pipe 14.
一氧化氮储罐30在开口处设置有气体节流阀30a，气体节流阀30a通过气密接头30b与气体输送管道31的第一端连通，气体输送管道31的第二端通过气体阀门31a与红细胞回收管道19相通；气泵32设置在气体输送管道31上，气泵32可通过制造气体输送管道31的管内压力差将一氧化氮储罐30中的一氧化氮气体泵入红细胞回收管道19。A gas throttle valve 30a is provided at the opening of the nitric oxide storage tank 30, and the gas throttle valve 30a is connected to the first end of the gas delivery pipeline 31 through an airtight joint 30b, and the second end of the gas delivery pipeline 31 is connected to the red blood cell recovery pipeline 19 through a gas valve 31a; an air pump 32 is provided on the gas delivery pipeline 31, and the air pump 32 can pump the nitric oxide gas in the nitric oxide storage tank 30 into the red blood cell recovery pipeline 19 by creating a pressure difference in the gas delivery pipeline 31.
血浆收集管道24的第一端通过带有节流阀的快速接头21b与血小板储罐21连通，血浆收集管道24的第二端通过快速接头24a与血浆收集袋25连通，血浆收集袋25可在第二次分离及冲洗结束、快速接头21b的阀门开启后接受暂存于血小板储罐21中的血浆残余成分，以便进一步处理。The first end of the plasma collection pipe 24 is connected to the platelet storage tank 21 through a quick connector 21b with a throttle valve, and the second end of the plasma collection pipe 24 is connected to the plasma collection bag 25 through a quick connector 24a. The plasma collection bag 25 can receive the residual plasma components temporarily stored in the platelet storage tank 21 after the second separation and flushing is completed and the valve of the quick connector 21b is opened for further processing.
回返管道22的第一端通过带有节流阀的快速接头21a与血小板储罐21连通，回返管道22的第二端通过带有节流阀的快速接头22a与五通管14的第五端连通；第五离心泵23设置在回返管道22上，第五离心泵23可以通过制造回返管道22与血小板储罐21之间的内部压力差，将第一次离心后暂存于血小板储罐21中的血小板血浆混合液经回返管道22泵回至五通管14，进而被生理盐水冲洗液经二通盖15重新冲入离心杯17以进行第二次离心。The first end of the return pipe 22 is connected to the platelet storage tank 21 through a quick connector 21a with a throttle valve, and the second end of the return pipe 22 is connected to the fifth end of the five-way pipe 14 through a quick connector 22a with a throttle valve; the fifth centrifugal pump 23 is arranged on the return pipe 22, and the fifth centrifugal pump 23 can create an internal pressure difference between the return pipe 22 and the platelet storage tank 21, and pump the platelet-plasma mixture temporarily stored in the platelet storage tank 21 after the first centrifugation back to the five-way pipe 14 through the return pipe 22, and then be flushed into the centrifuge cup 17 again through the two-way cover 15 by the physiological saline flushing liquid for a second centrifugation.
血小板回收管道27的第一端通过带有节流阀的快速接头21c与血小板储罐连通；血小板回收管道27的第二端设有多个末端，血小板回收管道27的第二端通过第一血小板回收管道阀门27a1与第一血小板回收支管27b1的第一端连通，第一血小板回收支管27b1的第二端通过快速接头27c1与第一血液有效成分冷藏箱连通；以此类推，血小板回收管道27的第n端通过第n血小板回收管道阀门27an与第n血小板回收支管27bn的第一端连通，第n血小板回收支管的第二端通过快速接头27cn与第n血液有效成分冷藏箱连通；第六离心泵26设置在血小板回收管道27上，第六离心泵26可以通过制造管内压力差，使得第二次离心后被冲入离心杯17内的血小板被吸入血小板回收管道27。The first end of the platelet recovery pipeline 27 is connected to the platelet storage tank through a quick connector 21c with a throttle valve; the second end of the platelet recovery pipeline 27 is provided with multiple ends, the second end of the platelet recovery pipeline 27 is connected to the first end of the first platelet recovery branch 27b1 through the first platelet recovery pipeline valve 27a1, and the second end of the first platelet recovery branch 27b1 is connected to the first blood active component refrigerator through the quick connector 27c1; by analogy, the nth end of the platelet recovery pipeline 27 is connected to the first end of the nth platelet recovery branch 27bn through the nth platelet recovery pipeline valve 27an, and the second end of the nth platelet recovery branch is connected to the nth blood active component refrigerator through the quick connector 27cn; the sixth centrifugal pump 26 is arranged on the platelet recovery pipeline 27, and the sixth centrifugal pump 26 can create a pressure difference in the tube so that the platelets flushed into the centrifugal cup 17 after the second centrifugation are sucked into the platelet recovery pipeline 27.
第一血液有效成分冷藏箱28-1通过快速接头19c1与第一红细胞回收支管19b1的第二端连通，第一血液有效成分冷藏箱28-1通过快速接头27c1与第一血小板回收管道27c1的第二端连通，第一血液有效成分冷藏箱28-1通过带有节流阀的快速接头28a1与第一输出管道的第一端连通；以此类推，第n血液有效成分冷藏箱28-n借快速接头19cn与第n红细胞回收支管19bn的第二端连通，第n血液有效成分冷藏箱28-n借快速接头27cn与第n血小板回收管道27cn的第二端连通，第n血液有效成分冷藏箱28-n借带有节流阀的快速接头28an与第n输出管道的第一端连通。The first blood active component refrigerator 28-1 is connected to the second end of the first red blood cell recovery branch 19b1 through the quick connector 19c1, the first blood active component refrigerator 28-1 is connected to the second end of the first platelet recovery pipeline 27c1 through the quick connector 27c1, and the first blood active component refrigerator 28-1 is connected to the first end of the first output pipeline through the quick connector 28a1 with a throttle valve; and so on, the nth blood active component refrigerator 28-n is connected to the second end of the nth red blood cell recovery branch 19bn through the quick connector 19cn, the nth blood active component refrigerator 28-n is connected to the second end of the nth platelet recovery pipeline 27cn through the quick connector 27cn, and the nth blood active component refrigerator 28-n is connected to the first end of the nth output pipeline through the quick connector 28an with a throttle valve.
上总输出管道29b的第一端设有多个末端，上总输出管道29b的第一端通过第一输出阀门29a1与第一输出管道29-1的第二端连通；以此类推，上总输出管道29b的第一端通过第n输出阀门29an与第n输出管道29-n的第二端连通；上总输出管道29b的第二端通过快速接头29c与体外膜肺装置33连通。The first end of the upper total output pipeline 29b is provided with multiple ends, and the first end of the upper total output pipeline 29b is connected to the second end of the first output pipeline 29-1 through the first output valve 29a1; by analogy, the first end of the upper total output pipeline 29b is connected to the second end of the nth output pipeline 29-n through the nth output valve 29an; the second end of the upper total output pipeline 29b is connected to the extracorporeal membrane lung device 33 through the quick connector 29c.
压缩机39与冷却风扇40设置在血液有效成分冷藏箱28-1～28-n附近区域；第1冷凝管道41-1～第n冷凝管道41-n设置在第一血液有效成分冷藏箱28-1～第n血液有效成分冷藏箱28-n的箱体外围，以保证处于工作状态时对冷藏箱内血液有效成分进行实时的充分冷却；第1冷凝管道阀门40a1～第n冷凝管道阀门40an分别设置在第一冷凝管道41-1～第n冷凝管道41-n的上端，以此使得操作者可以根据血液有效成分冷藏箱的实际需求量决定相对应的冷凝管道的开放状态，以此减少不必要的能源消耗；第1冷媒节流阀42-1～第n冷媒节流阀42-n分别设置在第一冷凝管道41-1～第n冷凝管道41-n的下端，以此使得操作者可以根据正在工作的血液有效成分冷藏箱的实际工作状态需要对相对应的冷凝管道的冷媒流量加以调整，从而实现冷藏温度的改变。The compressor 39 and the cooling fan 40 are arranged in the vicinity of the blood active component refrigerators 28-1 to 28-n; the first condensing pipe 41-1 to the nth condensing pipe 41-n are arranged on the periphery of the first blood active component refrigerator 28-1 to the nth blood active component refrigerator 28-n to ensure that the blood active components in the refrigerator are fully cooled in real time when in working state; the first condensing pipe valve 40a1 to the nth condensing pipe valve 40an are respectively arranged at the upper ends of the first condensing pipe 41-1 to the nth condensing pipe 41-n, so that the operator can determine the opening state of the corresponding condensing pipe according to the actual demand of the blood active component refrigerator, so as to reduce unnecessary energy consumption; the first refrigerant throttle valve 42-1 to the nth refrigerant throttle valve 42-n are respectively arranged at the lower ends of the first condensing pipe 41-1 to the nth condensing pipe 41-n, so that the operator can adjust the refrigerant flow of the corresponding condensing pipe according to the actual working state of the working blood active component refrigerator, thereby realizing the change of the refrigeration temperature.
体外膜肺装置33通过快速接头29c与上总输出管道29b的第二端连通；体外膜肺装置33通过快速接头33a与下总输出管道37的第一端连通；温水输入管道34的第一端与外部温水源相通，温水输入管道34的第二端通过快速接头34a与体外膜肺装置33内的水浴走行管道连通；输氧管道35的第一端与外部氧气源相通，输氧管道35的第二端通过快速接头35a与体外膜肺装置33内的血液有效成分流通管道相通；冷水输出管道36的第一端通过快速接头33b与体外膜肺装置33的水浴流通管道连通，冷水输出管道36的第二端与外部冷水接收装置连通；第七离心泵38设置在下总输出管道37上，第七离心泵38可以通过制造下总输出管道37的管内压力差，协助血液流出体外膜肺装置33并顺利输出至本装置外部，以供临床使用。The extracorporeal membrane lung device 33 is connected to the second end of the upper total output pipeline 29b through a quick connector 29c; the extracorporeal membrane lung device 33 is connected to the first end of the lower total output pipeline 37 through a quick connector 33a; the first end of the warm water input pipeline 34 is connected to the external warm water source, and the second end of the warm water input pipeline 34 is connected to the water bath running pipeline in the extracorporeal membrane lung device 33 through a quick connector 34a; the first end of the oxygen supply pipeline 35 is connected to the external oxygen source, and the second end of the oxygen supply pipeline 35 is connected to the blood effective component circulation pipeline in the extracorporeal membrane lung device 33 through a quick connector 35a; the first end of the cold water output pipeline 36 is connected to the water bath circulation pipeline of the extracorporeal membrane lung device 33 through a quick connector 33b, and the second end of the cold water output pipeline 36 is connected to the external cold water receiving device; the seventh centrifugal pump 38 is arranged on the lower total output pipeline 37, and the seventh centrifugal pump 38 can assist blood to flow out of the extracorporeal membrane lung device 33 and be smoothly output to the outside of the device for clinical use by creating a pressure difference in the lower total output pipeline 37.
实施例二Embodiment 2
基于前一实施例提供的用于快速分离、收集、低温储存供体血液的多功能血液回收装置，提出一种对血液进行快速分离、收集、低温储存的方法，具体过程包括：Based on the multifunctional blood recovery device for rapid separation, collection, and cryogenic storage of donor blood provided in the previous embodiment, a method for rapid separation, collection, and cryogenic storage of blood is proposed, and the specific process includes:
1)血液采集1) Blood collection
包括常规消毒、铺巾，行腹部大十字形入路依次切开皮肤、皮下肌层及腹膜，自动拉钩暴露腹腔肝下下腔静脉，对肝下下腔静脉行近端血管夹夹闭，远端结扎，在中间段距离夹闭位置较远处行一字形横向切口切开下腔静脉，将高通量血液采集管01的第一端向上插入切口以上的下腔静脉残端，确认插管无误后，固定高通量血液采集管01并将其第二端与第一管道06连通。The process includes routine disinfection, laying towels, and performing a large cruciate approach to the abdomen to sequentially incise the skin, subcutaneous muscle layer and peritoneum, using an automatic retractor to expose the inferior vena cava in the abdominal cavity, clamping the inferior vena cava at the proximal end with a vascular clamp, ligating the distal end, making a straight-line transverse incision in the middle section away from the clamping position to cut the inferior vena cava, inserting the first end of the high-throughput blood collection tube 01 upward into the stump of the inferior vena cava above the incision, and after confirming that the cannula is correctly inserted, fixing the high-throughput blood collection tube 01 and connecting its second end to the first pipeline 06.
2)血液净化，具体包括：2) Blood purification, including:
(1)连通各个管路：(1) Connect each pipeline:
将第一管道06与抗凝剂管道05的第二端连通，第一管道05的第二端与储血罐07连通，将第一负压吸引源06a与储血罐07连通；The first pipeline 06 is connected to the second end of the anticoagulant pipeline 05, the second end of the first pipeline 05 is connected to the blood storage tank 07, and the first negative pressure suction source 06a is connected to the blood storage tank 07;
将储血罐07与第二管道08连通，第二管道08与五通管14连通；将离心杯17与分离泵泵头16连通，二通盖15与分离泵泵头16连通；将二通盖15与将五通管14与二通盖15连通，二通盖15与第三管道20连通；将第三管道20与血小板储罐21连通，第二负压吸引源20a与血小板储罐21连通；The blood storage tank 07 is connected to the second pipeline 08, and the second pipeline 08 is connected to the five-way pipe 14; the centrifugal cup 17 is connected to the separation pump head 16, and the two-way cover 15 is connected to the separation pump head 16; the two-way cover 15 is connected to the five-way pipe 14 and the two-way cover 15, and the two-way cover 15 is connected to the third pipeline 20; the third pipeline 20 is connected to the platelet storage tank 21, and the second negative pressure suction source 20a is connected to the platelet storage tank 21;
将回返管道22与储血罐21连通，回返管道22与五通管14连通；将生理盐水储罐10与冲洗管道12连通，冲洗管道12与五通管14连通；将血浆收集管道24与血小板储罐21连通，血浆收集管道24与血浆收集袋25连通；The return pipe 22 is connected to the blood storage tank 21, and the return pipe 22 is connected to the five-way pipe 14; the physiological saline storage tank 10 is connected to the flushing pipe 12, and the flushing pipe 12 is connected to the five-way pipe 14; the plasma collection pipe 24 is connected to the platelet storage tank 21, and the plasma collection pipe 24 is connected to the plasma collection bag 25;
将红细胞回收管道19与五通管14连通，将一氧化氮储罐30与气体输送管道31连通，气体输送管道31与红细胞回收管道19连通；红细胞回收管道19与第一红细胞回收支管19b1～第n红细胞回收支管19bn分别连通，第一红细胞回收支管19b1～第n红细胞回收支管19bn分别与第一血液有效成分冷藏箱28-1～第n血液有效成分冷藏箱28-n连通；The red blood cell recovery pipeline 19 is connected to the five-way pipe 14, the nitric oxide storage tank 30 is connected to the gas delivery pipeline 31, and the gas delivery pipeline 31 is connected to the red blood cell recovery pipeline 19; the red blood cell recovery pipeline 19 is connected to the first red blood cell recovery branch pipe 19b1 to the nth red blood cell recovery branch pipe 19bn respectively, and the first red blood cell recovery branch pipe 19b1 to the nth red blood cell recovery branch pipe 19bn are connected to the first blood active component refrigerator 28-1 to the nth blood active component refrigerator 28-n respectively;
将血小板回收管道27与血小板储罐21连通，将血小板回收管道27与第一血小板回收支管27b1～第n血小板回收支管27bn分别连通，第一血小板回收支管27b1～第n血小板回收支管27bn分别与第一血液有效成分冷藏箱28-1～第n血液有效成分冷藏箱28-n连通；The platelet recovery pipe 27 is connected to the platelet storage tank 21, the platelet recovery pipe 27 is connected to the first platelet recovery branch pipe 27b1 to the nth platelet recovery branch pipe 27bn, respectively, and the first platelet recovery branch pipe 27b1 to the nth platelet recovery branch pipe 27bn are connected to the first blood active component refrigerator 28-1 to the nth blood active component refrigerator 28-n respectively;
将第一输出管道29-1～第n输出管道29-n分别与第一血液有效成分冷藏箱28-1～第n血液有效成分冷藏箱28-n连通，第一输出管道29-1～第n输出管道29-n分别与上总输出管道29b连通；将上总输出管道29b与体外膜肺装置连通，温水输入管道35与体外膜肺装置33连通，冷水输出管道36与体外膜肺装置33连通，输氧管道35与体外膜肺装置33连通，下总输出管道37与体外膜肺装置33连通。The first output pipeline 29-1 to the nth output pipeline 29-n are connected to the first blood active component refrigerator 28-1 to the nth blood active component refrigerator 28-n respectively, and the first output pipeline 29-1 to the nth output pipeline 29-n are connected to the upper total output pipeline 29b respectively; the upper total output pipeline 29b is connected to the extracorporeal membrane lung device, the warm water input pipeline 35 is connected to the extracorporeal membrane lung device 33, the cold water output pipeline 36 is connected to the extracorporeal membrane lung device 33, the oxygen supply pipeline 35 is connected to the extracorporeal membrane lung device 33, and the lower total output pipeline 37 is connected to the extracorporeal membrane lung device 33.
(2)血液收集(2) Blood collection
开启第一阀门05a、第一负压吸引源06a、快速接头07a的阀门，开启第二离心泵09；在第一负压吸引源06a的作用下，供体血液被抽入高通量血液采集管01，随后进入第一管道06，随后流入储血罐07并在此聚集。Open the first valve 05a, the first negative pressure suction source 06a, the valve of the quick connector 07a, and start the second centrifugal pump 09; under the action of the first negative pressure suction source 06a, the donor blood is drawn into the high-throughput blood collection tube 01, then enters the first pipeline 06, and then flows into the blood storage tank 07 and gathers there.
(3)血液净化(3) Blood purification
当供体血液开始流入储血罐07时，开启直线加速器43照射储血罐，以此在不破坏供体血液中的血细胞的同时有效杀灭血液中可能混有的游离肿瘤细胞。When the donor blood starts to flow into the blood storage tank 07, the linear accelerator 43 is turned on to irradiate the blood storage tank, thereby effectively killing free tumor cells that may be mixed in the blood without destroying the blood cells in the donor blood.
当储血罐07中的供体血液达到一定体积后，开启快速接头07a的阀门、快速接头08a的阀门，在第二离心泵09的作用下，储血罐07中的供体血液经第二管道08进入五通管14，此时快速接头08a、快速接头12a、快速接头14b、快速接头22a的节流阀均处于关闭状态，血液随即经二通盖15流入分离泵的离心杯17；在供体血液流入第二管道08时，开启血液透析装置44，供体血液陆续经过设置在第二管道08上的血液透析装置44与白细胞滤装置45，血液中的残余代谢废物与血液中可能引发免疫应答的白细胞成分被以此除去。When the donor blood in the blood storage tank 07 reaches a certain volume, the valves of the quick connector 07a and the quick connector 08a are opened. Under the action of the second centrifugal pump 09, the donor blood in the blood storage tank 07 enters the five-way pipe 14 through the second pipeline 08. At this time, the throttle valves of the quick connector 08a, the quick connector 12a, the quick connector 14b, and the quick connector 22a are all in a closed state, and the blood then flows into the centrifugal cup 17 of the separation pump through the two-way cover 15. When the donor blood flows into the second pipeline 08, the hemodialysis device 44 is opened, and the donor blood successively passes through the hemodialysis device 44 and the leukocyte filter device 45 arranged on the second pipeline 08, so that the residual metabolic waste in the blood and the leukocyte components in the blood that may induce immune response are removed.
3)红细胞成分分离与低温保存3) Separation and cryopreservation of red blood cell components
当离心杯中的供体血液达到一定体积时，暂时关闭快速接头07a的阀门、快速接头08a的阀门、第二离心泵09，启动分离泵转头16带动离心杯17及其中的供体血液进行高速旋转，进行第一次血液有效成分的分离；When the donor blood in the centrifugal cup reaches a certain volume, the valve of the quick connector 07a, the valve of the quick connector 08a and the second centrifugal pump 09 are temporarily closed, and the separation pump rotor 16 is started to drive the centrifugal cup 17 and the donor blood therein to rotate at a high speed to perform the first separation of the effective components of the blood;
第一次血液有效成分的分离结束后，红细胞成分位于离心杯17的外周侧，血小板、血浆则位于离心杯17的中心侧，此时开启生理盐水节流阀11、快速接头12a的阀门，开启第三离心泵13，开启第二负压吸引源20a，生理盐水冲洗液经冲洗管道12流入五通管，再经二通盖25流入分离泵的离心杯17，在此与位于离心杯17中心侧的血浆、血小板混合后，在第二负压吸引源20a的作用下，血浆、血小板成分与生理盐水的混合液一同经第三管道20流入血小板储罐；After the first separation of effective blood components is completed, the red blood cell components are located on the outer side of the centrifugal cup 17, and the platelets and plasma are located on the central side of the centrifugal cup 17. At this time, the saline throttle valve 11 and the valve of the quick connector 12a are opened, the third centrifugal pump 13 is opened, and the second negative pressure suction source 20a is opened. The saline flushing liquid flows into the five-way pipe through the flushing pipe 12, and then flows into the centrifugal cup 17 of the separation pump through the two-way cover 25. After being mixed with the plasma and platelets located on the central side of the centrifugal cup 17, under the action of the second negative pressure suction source 20a, the mixed liquid of the plasma, platelet components and the saline flows into the platelet storage tank through the third pipe 20;
此时红细胞成分仍留在离心杯17的外周侧，关闭第二负压吸引源20a，关闭快速接头08a、快速接头12a的阀门，开启快速接头14b的阀门，开启第1红细胞回收管道阀门19a1～第m红细胞回收管道阀门19am(在本实施例中，m在此表示根据实际需要确定的用于保存红细胞成分的血液有效成分冷藏箱总使用数量，下同，其中m满足1≤m≤n)，开启一氧化氮储罐阀门30a、气体输送管道阀门30b并调整气体输送管道31中的一氧化氮的流量，开启第四离心泵18、气泵32，在气泵32的作用下，一氧化氮储罐30中的适量一氧化氮气体经气体输送管道31从气体输送管道阀门31a处输入红细胞回收管道19并与其中的红细胞成分混合；At this time, the red blood cell components still remain on the outer peripheral side of the centrifugal cup 17, the second negative pressure suction source 20a is closed, the valves of the quick connector 08a and the quick connector 12a are closed, the valve of the quick connector 14b is opened, the first red blood cell recovery pipeline valve 19a1 to the mth red blood cell recovery pipeline valve 19am are opened (in this embodiment, m here represents the total number of blood effective component refrigerators used to store red blood cell components determined according to actual needs, the same below, where m satisfies 1≤m≤n), the nitric oxide storage tank valve 30a and the gas delivery pipeline valve 30b are opened, and the flow rate of nitric oxide in the gas delivery pipeline 31 is adjusted, the fourth centrifugal pump 18 and the air pump 32 are opened, and under the action of the air pump 32, an appropriate amount of nitric oxide gas in the nitric oxide storage tank 30 is input into the red blood cell recovery pipeline 19 from the gas delivery pipeline valve 31a through the gas delivery pipeline 31 and mixed with the red blood cell components therein;
红细胞成分在第四离心泵18的作用下，经红细胞回收管道19、第1红细回收支管19b1～第m红细胞回收支管19bm流入第xRBC血液有效成分冷藏箱28-xRBC～第28-yRBC血液有效成分冷藏箱28-yRBC并在此聚集(在本实施例中，xRBC表示按编号数值从小到大依次排序，第一个被选定用于盛放红细胞成分的血液有效成分冷藏箱的编号，yRBC表示按编号数值从小到大依次排序，最后一个被选定用于盛放红细胞成分的血液有效成分冷藏箱的编号，其中xRBC、yRBC满足1≤xRBC＜yRBC≤m)；在红细胞成分全部进入第zRBC(其中zRBC满足1≤xRBC≤zRBC≤yRBC≤m)血液有效成分冷藏箱28-zRBC后，开启压缩机39，开启对应的第zRBC冷凝管道阀门40azRBC，开启对应的第zRBC冷媒节流阀42-zRBC并调整第zRBC冷凝管道41-zRBC的冷媒流量，控制第zRBC血液有效成分冷藏箱28-zRBC的温度，从而实现第zRBC血液有效成分冷藏箱28-zRBC对其中的红细胞成分的低温冷藏功能。Under the action of the fourth centrifugal pump 18, the red blood cell components flow into the xthRBC blood effective component refrigerator 28-xRBC to the 28-ythRBC blood effective component refrigerator 28-yRBC through the red blood cell recovery pipe 19 and the first red blood cell recovery branch pipe 19b1 to the mth red blood cell recovery branch pipe 19bm and gather there (in the present embodiment, xRBC represents the number of the first blood effective component refrigerator selected for containing red blood cell components in ascending order according to the number value, and yRBC represents the number of the last blood effective component refrigerator selected for containing red blood cell components in ascending order according to the number value, wherein xRBC and yRBC satisfy 1≤xRBC <yRBC ≤m); after all the red blood cell components enter the zthRBC (where zRBC satisfies 1≤xRBC ≤zRBC ≤yRBC ≤m) blood effective component refrigerator 28-zRBC , the compressor 39 is turned on, the corresponding zthRBC condensation pipe valve 40az RBC is opened, and the corresponding zth RBC condensation pipe valve 40azRBC is opened.The RBC refrigerant throttle valve 42-zRBC adjusts the refrigerant flow of the zthRBC condensation pipe 41-zRBC to control the temperature of the zthRBC blood effective component refrigerator 28-zRBC , thereby realizing the low-temperature refrigeration function of the zthRBC blood effective component refrigerator 28-zRBC for the red blood cell components therein.
4)血小板成分分离与低温保存4) Platelet component separation and cryopreservation
与此同时，在红细胞成分完全流出五通管14后，对于暂存于血小板储罐21中的血小板与血浆成分，关闭快速接头14b的阀门，开启快速接头21a的阀门、快速接头22a的阀门，开启第五离心泵23，血小板与血浆成分在第五离心泵23的作用下，经回返管道22再次流入五通管15，随即再次经二通盖15流入离心杯17；At the same time, after the red blood cell components completely flow out of the five-way pipe 14, for the platelets and plasma components temporarily stored in the platelet storage tank 21, the valve of the quick connector 14b is closed, the valve of the quick connector 21a and the valve of the quick connector 22a are opened, and the fifth centrifugal pump 23 is turned on. Under the action of the fifth centrifugal pump 23, the platelets and plasma components flow into the five-way pipe 15 again through the return pipe 22, and then flow into the centrifugal cup 17 again through the two-way cover 15;
当离心杯中的血小板与血浆成分达到一定体积时，暂时关闭快速接头22a的阀门，暂时关闭第五离心泵23，再次启动分离泵转头16带动离心杯17及其中的供体血液进行高速旋转，进行第二次血液有效成分的分离；When the platelet and plasma components in the centrifugal cup reach a certain volume, the valve of the quick connector 22a is temporarily closed, the fifth centrifugal pump 23 is temporarily closed, and the separation pump rotor 16 is started again to drive the centrifugal cup 17 and the donor blood therein to rotate at a high speed, so as to separate the effective components of the blood for the second time;
第二次血液有效成分的分离结束后，血小板成分位于离心杯17的外周侧，血浆成分则位于离心杯17的中心侧，此时再次开启生理盐水节流阀11、快速接头12a的阀门，开启第三离心泵13，开启第二负压吸引源20a，生理盐水冲洗液经冲洗管道12流入五通管，再经二通盖25流入分离泵的离心杯17，在此与位于离心杯17中心侧的血浆成分混合后，在第二负压吸引源20a的作用下，血浆成分与生理盐水的混合液一同经第三管道20再次流入血小板储罐21；After the second separation of effective blood components is completed, the platelet components are located on the outer side of the centrifugal cup 17, and the plasma components are located on the central side of the centrifugal cup 17. At this time, the saline throttle valve 11 and the valve of the quick connector 12a are opened again, the third centrifugal pump 13 is opened, and the second negative pressure suction source 20a is opened. The saline flushing liquid flows into the five-way pipe through the flushing pipe 12, and then flows into the centrifugal cup 17 of the separation pump through the two-way cover 25. After being mixed with the plasma components located on the central side of the centrifugal cup 17, under the action of the second negative pressure suction source 20a, the mixed liquid of the plasma components and the saline flows into the platelet storage tank 21 again through the third pipe 20;
关闭第二负压吸引源20a，开启快速接头21b的阀门，此时快速接头21a与快速接头21c的阀门处于关闭状态，血小板储罐中的血浆成分与生理盐水混合液经血浆收集管道24流入血浆收集袋25；持续开启生理盐水节流阀11和快速接头21b的阀门，至生理盐水冲洗液将血小板储罐21中的血浆成分完全冲洗进入血浆收集袋25后，关闭生理盐水节流阀11和快速接头21b的阀门；The second negative pressure suction source 20a is closed, and the valve of the quick connector 21b is opened. At this time, the valves of the quick connector 21a and the quick connector 21c are in a closed state, and the plasma components in the platelet storage tank and the physiological saline mixture flow into the plasma collection bag 25 through the plasma collection pipe 24; the physiological saline throttle valve 11 and the valve of the quick connector 21b are continuously opened until the physiological saline flushing liquid completely flushes the plasma components in the platelet storage tank 21 into the plasma collection bag 25, and then the physiological saline throttle valve 11 and the valve of the quick connector 21b are closed;
开启第二负压吸引源20a，在第二负压吸引源20a的作用下，继血浆成分与生理盐水混合液之后，位于分离泵离心杯12外周侧的血小板也经第三管道20流入血小板储罐21；The second negative pressure suction source 20a is turned on. Under the action of the second negative pressure suction source 20a, the platelets located on the outer peripheral side of the separation pump centrifugal cup 12 also flow into the platelet storage tank 21 through the third pipe 20 after the plasma component and the physiological saline mixture;
关闭第二负压吸引源20a，开启快速接头21c的阀门，开启第1血小板回收管道阀门27a1～第t血小板回收管道阀门27at(在本实施例中，t在此表示根据实际需要确定的用于保存血小板成分的血液有效成分冷藏箱总使用数量，下同，其中t满足1≤t≤n)，开启第六离心泵26，血小板成分在第六离心泵26的作用下，经血小板回收管道27、第1血小板回收支管27b1～第m红细胞回收支管27bt流入第xPLT血液有效成分冷藏箱28-xPLT～第yPLT血液有效成分冷藏箱28-yPLT并在此聚集(在本实施例中，xPLT表示按编号数值从小到大依次排序，第一个被选定用于盛放血小板成分的血液有效成分冷藏箱的编号，yPLT表示按编号数值从小到大依次排序，最后一个被选定用于盛放血小板成分的血液有效成分冷藏箱的编号，其中xPLT、yPLT满足1≤xPLT＜yPLT≤t)；在血小板成分全部进入第zPLT(其中zPLT满足1≤xPLT≤zPLT≤yPLT≤t)血液有效成分冷藏箱后，压缩机39已经启动，开启对应的第zPLT冷凝管道阀门40azPLT，开启对应的第zPLT冷媒节流阀42-zPLT并调整第zPLT冷凝管道41-zPLT的冷媒流量，控制第zPLT血液有效成分冷藏箱28-zPLT的温度，从而实现第zPLT血液有效成分冷藏箱28-zPLT对其中的血小板成分的低温冷藏功能。The second negative pressure suction source 20a is closed, the valve of the quick connector 21c is opened, the first platelet recovery pipeline valve 27a1 to the t-th platelet recovery pipeline valve 27at are opened (in the present embodiment, t here represents the total number of blood effective component refrigerators used to store platelet components determined according to actual needs, the same below, wherein t satisfies 1≤t≤n), and the sixth centrifugal pump 26 is turned on. Under the action of the sixth centrifugal pump 26, the platelet components flow into the x-thPLT blood effective component refrigerator 28-xPLT to the y-thPLT blood effective component refrigerator 28-yPLT through the platelet recovery pipeline 27 and the first platelet recovery branch pipe 27b1 to the m-th red blood cell recovery branch pipe 27bt and are gathered there (in the present embodiment, xPLT represents the number of the first blood effective component refrigerator selected for storing platelet components in ascending order of number value, and yPLT represents the number of the last blood effective component refrigerator selected for storing platelet components in ascending order of number value, wherein xPLT , yPLT satisfies 1≤xPLT ＜yPLT ≤t); after all the platelet components enter the zPLT (wherein zPLT satisfies 1≤xPLT ≤zPLT ≤yPLT ≤t) blood effective component refrigerator, the compressor 39 has been started, the corresponding zPLT condensation pipeline valve 40azPLT is opened, the corresponding zPLT refrigerant throttle valve 42-zPLT is opened and the refrigerant flow of the zPLT condensation pipeline 41-zPLT is adjusted to control the temperature of the zPLT blood effective component refrigerator 28-zPLT , thereby realizing the low-temperature refrigeration function of the zPLT blood effective component refrigerator 28-zPLT for the platelet components therein.
5)当离心杯中的血小板成分完全流出后，重新开启快速接头07a的阀门、快速接头08a的阀门、第二离心泵09，通过第二管道08重新开始对血液进行离心，并重复红细胞和血小板的分享和低温保存。5) When the platelet components in the centrifuge cup have completely flowed out, reopen the valve of the quick connector 07a, the valve of the quick connector 08a, and the second centrifugal pump 09, restart the centrifugation of the blood through the second pipe 08, and repeat the sharing and low-temperature storage of red blood cells and platelets.
6)有效成分的快速复苏6) Rapid recovery of active ingredients
在需要将在第zRBC(其中zRBC满足1≤xRBC≤zRBC≤yRBC≤m)血液有效成分冷藏箱28-zRBC低温冻存的红细胞成分输出冷藏设备以另做使用时，开启第28-zRBC冷藏箱快速接头，开启第28-zRBC输出阀门，开启第七离心泵38，在第七离心泵38的作用下，储存于第zRBC血液有效成分冷藏箱28-zRBC中的红细胞成分经第zRBC输出管道29-zRBC、上总输出管道29b进入体外膜肺装置33中的血液有效成分流通管道；When it is necessary to output the red blood cell components cryopreserved in the zRBC (where zRBC satisfies 1≤xRBC ≤zRBC ≤yRBC ≤m) blood active component refrigerator 28-zRBC to the refrigeration device for further use, the 28-zRBC refrigerator quick connector is opened, the 28-zRBC output valve is opened, and the seventh centrifugal pump 38 is opened. Under the action of the seventh centrifugal pump 38, the red blood cell components stored in the zRBC blood active component refrigerator 28-zRBC enter the blood active component circulation pipeline in the extracorporeal membrane lung device 33 through the zRBC output pipeline 29-zRBC and the upper total output pipeline 29b;
进一步地，温水从装置外部经温水输入管道34流入体外膜肺装置33中的水浴流通管道，与血液有效成分流通管道中的红细胞成分同向并列呈螺旋形流动，此过程中水浴流通管道中的温水与血液有效成分流通管道中的红细胞成分发生热交换，低温的红细胞成分复温从而实现低温冻存的红细胞的复苏，温水则因放热转变为冷水，随后完成热交换的冷水经冷水输出管道36流出体外膜肺装置33中的水浴流通管道；与此同时，装置外部的氧气源产生氧气，氧气经输氧管道35进入体外膜肺装置33中的血液有效成分流通管道并与红细胞成分混匀，使红细胞携氧量增加；在经过体外膜肺装置33后，红细胞成分经下总输出管道37输出至外部待用。Furthermore, warm water flows from the outside of the device through the warm water input pipe 34 into the water bath circulation pipe in the extracorporeal membrane lung device 33, and flows in the same direction and in parallel with the red blood cell components in the blood effective component circulation pipe in a spiral shape. In this process, the warm water in the water bath circulation pipe exchanges heat with the red blood cell components in the blood effective component circulation pipe, and the low-temperature red blood cell components are rewarmed to achieve the recovery of the low-temperature frozen red blood cells. The warm water is converted into cold water due to heat release, and then the cold water that has completed the heat exchange flows out of the water bath circulation pipe in the extracorporeal membrane lung device 33 through the cold water output pipe 36; at the same time, the oxygen source outside the device generates oxygen, and the oxygen enters the blood effective component circulation pipe in the extracorporeal membrane lung device 33 through the oxygen supply pipe 35 and is mixed with the red blood cell components, so that the oxygen carrying capacity of the red blood cells is increased; after passing through the extracorporeal membrane lung device 33, the red blood cell components are output to the outside through the lower total output pipe 37 for standby use.
以上实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。The technical features of the above embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
以上所述仅为本发明的优选实施方式而已，并不用于限制本发明，对于本领域的技术人员来说，本发明可以有各种更改和变化。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
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| CN202311853942.XACN117695460B (en) | 2023-12-29 | 2023-12-29 | Multifunctional blood recovery device and application method thereof |
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| CN202311853942.XACN117695460B (en) | 2023-12-29 | 2023-12-29 | Multifunctional blood recovery device and application method thereof |
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