Disclosure of Invention
The invention mainly aims to provide a reaction box and an extraction reaction device, which aim to reduce detection cost, improve timeliness and reduce operation difficulty.
To achieve the above object, the present invention provides a reaction cassette comprising:
a first runner assembly provided with an injection port and a first runner adapted for insertion of a syringe; and
the second runner subassembly, with first runner subassembly is connected fixedly, second runner subassembly is equipped with second runner, reaction tank and mounting groove, the one end of first runner is used for supplying pure water inflow, the other end of first runner with the reaction tank intercommunication, the one end of second runner with the injection port intercommunication, the other end of second runner with the reaction tank intercommunication, the mounting groove with the reaction tank intercommunication, be equipped with the test strip in the mounting groove.
Optionally, the first runner assembly includes a first runner plate and locates pure water package on the first runner plate, the injection mouth with first runner is all located on the first runner plate, still be equipped with on the first runner plate and puncture the structure in the position of corresponding to the pure water package, so as to puncture when pressing the pure water package makes the pure water warp first runner inflow in the reaction tank.
Optionally, the reaction box further comprises a baffle plate for blocking the liquid outlet end of the reaction tank, the first flow channel plate is provided with a socket for the movable insertion of the baffle plate and a differential pressure bridge which is positioned on one side of the socket and protrudes inwards, the middle part of the differential pressure bridge is provided with a permeation channel, one end of the permeation channel is abutted to the baffle plate, and the other end of the permeation channel is communicated with the mounting groove.
Optionally, the second flow channel assembly includes a second flow channel plate and a puncture needle disposed on the second flow channel plate and corresponding to the injection port, the puncture needle is used for puncturing silica gel of the injector, the second flow channel, the reaction tank and the mounting tank are all disposed on the second flow channel plate, a slot for movably inserting the baffle is disposed on the second flow channel plate corresponding to the position of the insertion port, and a communication port for communicating the permeation channel is disposed on the other side of the slot; when the baffle is inserted, the reaction groove is not communicated with the mounting groove; when the baffle is not inserted, the reaction tank is communicated with the mounting tank; and/or the height of the bottom of the reaction tank is greater than that of the mounting tank.
Optionally, the second flow channel comprises a main flow channel and at least two equally divided branches communicated with the main flow channel, a buffer groove for buffering liquid is arranged on the main flow channel, and a flow speed blocking rib for reducing the flow speed of the liquid and an acceleration slope for increasing the flow speed of the liquid are arranged in the equally divided branches; the reaction tank at least comprises a single-time reaction sub tank and a double-time reaction sub tank, the volume of the double-time reaction sub tank is twice that of the single-time reaction sub tank, at least one equipartition branch is communicated with the single-time reaction sub tank, and at least one equipartition branch is communicated with the double-time reaction sub tank.
Optionally, the reaction box further comprises a circuit component, wherein the circuit component and the second flow channel component are in an integrated structure or a split structure, and the circuit component is used for heating and vibrating the mixed liquid in the reaction tank.
Optionally, the circuit assembly includes the casing subassembly and locates battery, circuit board, the piece that generates heat and vibration motor in the casing subassembly, the circuit board with the battery electricity is connected, the piece that generates heat with vibration motor respectively with the circuit board electricity is connected, the circuit board is used for controlling the piece that generates heat with vibration motor work.
Optionally, when the circuit assembly and the second flow channel assembly are in an integrated structure, a containing groove for containing the circuit assembly is formed in one side, facing away from the second flow channel, of the second flow channel assembly, a bottom plate is arranged on the upper cover of the containing groove, and the second flow channel assembly and the bottom plate form the shell assembly; or alternatively
When the circuit assembly and the second flow channel assembly are of a split type structure, the shell assembly comprises a first shell and a second shell fixedly connected with the first shell, the first shell and the second shell enclose an installation space for installing the circuit assembly, a butt joint block is convexly arranged on the first shell, a butt joint groove is formed in the position, corresponding to the butt joint block, of the second flow channel assembly, a heating part is exposed out of the first shell, a groove for the heating part to extend into is formed in the position, corresponding to the heating part, of the second flow channel assembly, and the groove and the reaction groove are arranged back to back.
In order to achieve the above object, the present invention also provides an extraction reaction apparatus comprising:
a reaction cassette as described above; and
an injector which is provided independently of the reaction cassette, and which is insertable into an injection port of the reaction cassette to inject a liquid to be detected into the reaction cassette;
the reaction cassette includes:
a first runner assembly provided with an injection port and a first runner adapted for insertion of a syringe; and
the second runner subassembly, with first runner subassembly is connected fixedly, second runner subassembly is equipped with second runner, reaction tank and mounting groove, the one end of first runner is used for supplying pure water inflow, the other end of first runner with the reaction tank intercommunication, the one end of second runner with the injection port intercommunication, the other end of second runner with the reaction tank intercommunication, the mounting groove with the reaction tank intercommunication, be equipped with the test strip in the mounting groove.
Optionally, the injector comprises an injector body, a protective cover, a push rod and a lock catch; the protective cover is arranged on the injection end of the injector body, silica gel is injected into plastic cement by adopting a double-shot injection molding process, and the protective cover is provided with sealing ribs for sealing the injection end of the injector body; the push rod is inserted into the injector body, and is in a round rod shape; the lock catch is movably arranged on the end part of the injector body and is inserted into the push rod so as to lock the push rod.
In the technical scheme of the invention, the reaction box comprises a first flow channel component and a second flow channel component, wherein the first flow channel component is provided with an injection port and a first flow channel which are suitable for the insertion of a syringe; the second runner subassembly is connected fixedly with first runner subassembly, and second runner subassembly is equipped with second runner, reaction tank and mounting groove, and the one end of first runner is used for supplying pure water inflow, and the other end and the reaction tank of first runner communicate, and the one end and the injection mouth of second runner communicate, and the other end and the reaction tank of second runner communicate, and the mounting groove communicates with the reaction tank, is equipped with the test strip in the mounting groove. It can be appreciated that the invention provides a reaction box, when in use, a user can put a cotton stick dipped with an antigen into a lysis solution of an injector by himself, after fully mixing, the injector is inserted into an injection port, then the mixed solution flows into a reaction tank to be mixed with pure water for reaction, and the mixed solution can flow into a test strip after vibrating, heating and amplifying, and a detection result can be obtained after a period of time. Therefore, the invention realizes rapid test, can accurately judge in a short time, greatly improves timeliness, has low operation difficulty, does not need to be equipped with professional medical care or detection personnel, does not need to build a collection site or laboratory, and greatly reduces the cost of detecting nucleic acid and the like.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without any inventive effort, are intended to be within the scope of the invention.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present invention are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.
Furthermore, the description of "first," "second," etc. in this disclosure is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" as it appears throughout is meant to include three side-by-side schemes, for example, "A and/or B", including the A scheme, or the B scheme, or the scheme where A and B meet at the same time. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present invention.
The invention provides a reaction box which can be used for nucleic acid detection, antigen-antibody detection, virus detection, protein detection and the like, and is not limited herein.
Referring to fig. 1 to 8, in an embodiment of the present invention, the reaction cassette 100 is applied to an antigen-antibody (colloidal gold) extraction reaction apparatus, the extraction reaction apparatus including a syringe 200, the reaction cassette 100 including a first flow path assembly 10 and a second flow path assembly 20, the first flow path assembly 10 being provided with an injection port 10a and a first flow path 10b adapted for insertion of the syringe 200; the second flow channel assembly 20 is fixedly connected with the first flow channel assembly 10, the second flow channel assembly 20 is provided with a second flow channel 20a, a reaction tank 20b and a mounting tank 20c, one end of the first flow channel 10b is used for allowing pure water to flow in, the other end of the first flow channel 10b is communicated with the reaction tank 20b, one end of the second flow channel 20a is communicated with the injection port 10a, the other end of the second flow channel 20a is communicated with the reaction tank 20b, the mounting tank 20c is communicated with the reaction tank 20b, and at least one test strip 30 is arranged in the mounting tank 20 c.
In this embodiment, the first flow channel assembly 10 may be formed by the upper case of the reaction cassette 100, the second flow channel assembly 20 may be formed by the lower case of the reaction cassette 100, or two flow channel structures respectively provided in the case, which is not limited herein.
As shown in fig. 1 and 2, the injection port 10a of the first flow channel assembly 10 may be disposed in a fastening structure matched with the syringe 200, so as to be in butt joint with the syringe 200, thereby avoiding leakage and being beneficial to improving the convenience and safety of detection.
It can be appreciated that the present invention provides a reaction cassette 100, in which a user can self-put a swab dipped with an antigen into a lysis solution of a syringe 200, fully mix the swab, insert the syringe 200 into an injection port 10a, flow the mixed solution into a reaction tank 20b under the action of gravity to mix with pure water for reaction, vibrate, heat and amplify the mixed solution, flow the mixed solution onto a test strip 30, and obtain a detection result after a period of time. Therefore, the invention realizes rapid test, can accurately judge in a short time, greatly improves timeliness, has low operation difficulty, does not need to be equipped with professional medical care or detection personnel, does not need to build a collection site or laboratory, and greatly reduces the cost of detecting nucleic acid and the like.
In order to further improve the detection efficiency and ensure the accuracy of the detection result, referring mainly to fig. 3 to 5, in an embodiment, the first flow channel assembly 10 may include a first flow channel plate 11 and a pure water bag 12 disposed on the first flow channel plate 11, where the injection port 10a and the first flow channel 10b are disposed on the first flow channel plate 11, and a piercing structure 111 is disposed on the first flow channel plate 11 at a position corresponding to the pure water bag 12, so as to pierce the pure water bag 12 when the pure water bag 12 is pressed, so that pure water flows into the reaction tank 20b through the first flow channel 10 b. The first flow channel 10b may be disposed in an omega shape, and the first flow channel 10b may be disposed around the piercing structure 111 and may extend to the center of the piercing structure 111. A protective film 13 may be provided between the first flow passage 10b of the first flow passage plate 11 and the pure water bag 12 to prevent the pure water from overflowing.
In use, after the pure water bag 12 is pressed with force, pure water flows into the reaction tank 20b through the first flow path 10b, and the mixed solution in the reaction tank 20b is diluted.
The invention replaces additional equipment to dilute by arranging the pure water bag 12 structure, thereby effectively reducing the loss and the cost. Meanwhile, the reaction box 100 is provided with colloidal gold body, so that the timeliness is high, and the reaction result can be obtained in a short time.
In this embodiment, the pure water bag 12 may be fixed on the first flow channel plate 11 by means of adhesion or clamping, and the pure water bag 12 may be a flexible bag structure that is easy to puncture, and the specific material is not limited. The piercing structure 111 may be a pointed block or a saw tooth protruding from the first flow channel plate 11, and is preferably a plurality of pointed blocks, and the plurality of pointed blocks are surrounded by a circle, which is not limited herein.
In this embodiment, the first flow channel plate 11 may further be provided with an observation window 10c, where the observation window 10c is disposed corresponding to the result display position of the test strip 30, so as to obtain the detection result in time.
In addition, in some other embodiments, the dilution liquid may be provided by an external pure water supply device or by adding pure water to the first flow channel 10b during the detection process, which is also within the inventive concept of the present application.
Referring to fig. 2 to 5 and 8, in an embodiment, the reaction box 100 may further include a baffle 40 for blocking the liquid outlet end of the reaction tank 20b, the first flow channel plate 11 is provided with a socket 11a into which the baffle 40 is movably inserted, and a differential pressure bridge 112 disposed at one side of the socket 11a and protruding inwards, a permeation channel is disposed in the middle of the differential pressure bridge 112, one end of the permeation channel abuts against the baffle 40, and the other end of the permeation channel is communicated with the mounting groove 20 c. In this way, it is ensured that the mixed solution in the reaction tank 20b can complete amplification, after the amplification is completed (about 10 minutes), the user can withdraw the baffle 40, and the liquid rapidly flows onto the test strip 30 through the differential pressure bridge 112, so that the accuracy of the detection result is effectively ensured, and the detection efficiency is further improved.
Based on the above embodiment, as shown in fig. 2 to 8, the second flow channel assembly 20 may include a second flow channel plate 21 and a puncture needle 22 disposed on the second flow channel plate 21 and corresponding to the injection port 10a, the puncture needle 22 extending into the injection port 10a, the puncture needle 22 being used for puncturing the silica gel of the injector 200, the second flow channel 20a, the reaction tank 20b and the mounting tank 20c being disposed on the second flow channel plate 21, a slot for movably inserting the baffle 40 being disposed on the second flow channel plate 21 corresponding to the insertion opening 11a, and a communication port for communicating with the permeation channel being disposed on the other side of the slot; when the baffle 40 is inserted in place, the reaction tank 20b is not in communication with the installation tank 20 c; when the baffle 40 is not inserted or not inserted in place, the reaction tank 20b communicates with the installation tank 20 c. Wherein, the second runner 20a of the second runner plate 21 may also be covered with a sealing film to enhance the sealing property.
In this embodiment, a silica gel ring may be disposed at the insertion end of the baffle 40 to improve the sealing performance of the sealing slot of the baffle 40, so as to prevent the mixed solution from penetrating into the test strip 30 in advance.
Referring mainly to fig. 8, in this embodiment, the height of the bottom of the reaction tank 20b may be greater than the height of the bottom of the installation tank 20c, that is, the depth of the reaction tank 20b is smaller than the depth of the installation tank 20c, so that a high-low pressure difference may be further formed, and after the amplification is completed, the liquid may flow to the target position on the test strip 30 more rapidly, so as to further improve the detection efficiency and accuracy.
In order to further improve accuracy of the detection result, referring to fig. 3, in this embodiment, the second flow channel 20a may include a main flow channel 20a1 and at least two equally divided sub-flows 20a2 that are communicated with the main flow channel 20a1, a buffer groove 20a11 for buffering liquid is disposed on the main flow channel 20a1, and a flow velocity blocking rib 20a21 for reducing the flow velocity of the liquid and an acceleration slope for increasing the flow velocity of the liquid are disposed in the equally divided sub-flows 20a 2; the reaction tank 20b includes at least a single-time reaction sub-tank 20b1 and a double-time reaction sub-tank 20b2, the double-time reaction sub-tank 20b2 has a volume twice that of the single-time reaction sub-tank 20b1, at least one equally divided sub-stream 20a2 is in communication with the single-time reaction sub-tank 20b1, and at least one equally divided sub-stream 20a2 is in communication with the double-time reaction sub-tank 20b 2. In addition, in order to ensure that a proper amount of liquid flows into the test strip 30 to make the detection result more accurate, the waste liquid tank 20b3 communicating with the branch sharing flow 20a2 may be provided on the second flow path plate 21 in this embodiment to store the surplus waste liquid.
The runner structure has the advantages of replacing extra large equipment for uniform division, avoiding separation of deep pore plates, magnetic rod sleeves and the like, and reducing loss and use cost.
The reaction cassette 100 of the present invention itself adopts the microfluidic technology, and utilizes the capillary principle (i.e., according to the theory of thermodynamics, a substance is sought to exist in the lowest energy state, and hydrogen bonding is a means of reducing chemical energy.) water is a polar substance and thus can form hydrogen bonding inside, which makes it have many unique properties, here, actually shows hydrophilicity and hydrophobicity), the mixed solution is equally divided in the equally divided sub-streams 20a2, and after the pure water pack 12 is strongly pressed, the pure water flows into the reaction tank 20b through the first flow channel 10b, and the mixed solution is diluted by the pure water.
To achieve the heating and vibration functions to further ensure the accuracy of the detection result and reduce the detection cost, in some embodiments, the reaction cassette 100 may further include a circuit assembly 300, where the circuit assembly 300 and the second flow path assembly 20 are integrated (as shown in fig. 9 to 11) or separated (as shown in fig. 1 to 8), and the circuit assembly 300 is used to heat and vibrate the mixed solution in the reaction tank 20 b.
Referring mainly to fig. 6 to 8 and 11, in the present embodiment, the circuit assembly 300 may include a housing assembly 31, a battery 32 disposed in the housing assembly 31, a circuit board (not shown), a heating element 34 and a vibration motor 35, wherein the circuit board is electrically connected with the battery 32, the heating element 34 and the vibration motor 35 are respectively electrically connected with the circuit board, and the circuit board is used for controlling the heating element 34 and the vibration motor 35 to work.
The heating element 34 may be a plurality of heating aluminum sheets or heating rods, etc., and the heating temperature may be set to 45 ℃, which is not limited herein.
In the first case, that is, when the circuit assembly 300 and the second flow channel assembly 20 are integrally formed, as shown in fig. 9 to 11, a receiving groove for receiving the circuit assembly 300 is formed on a side of the second flow channel assembly 20 opposite to the second flow channel 20a, a bottom plate 311 is disposed on a bottom cover of the receiving groove, and the second flow channel assembly 20 and the bottom plate 311 together form the housing assembly 31. Therefore, the operation steps can be further simplified, and the user can conveniently and automatically detect the detection.
In the second case, that is, when the circuit assembly 300 and the second flow channel assembly 20 are in a split structure, as shown in fig. 1 to 8, the housing assembly 31 includes a first housing 321 and a second housing 322 connected and fixed with the first housing 321, the first housing 321 and the second housing 322 enclose an installation space for installing the power supply assembly 300, the first housing 321 is convexly provided with a butt joint block 301, the second flow channel assembly 20 is provided with a butt joint groove corresponding to the position of the butt joint block 301, the heating element 34 is partially exposed outside the first housing 321, the second flow channel assembly 20 is provided with a groove for extending into the second flow channel assembly corresponding to the position of the heating element 34, and the groove and the reaction groove 20b are arranged back to back. Thus, the convenience of assembly can be improved, mass production and manufacture can be facilitated, and the reaction cassette 100 is relatively simple to use compared with the prior art.
The present invention also proposes an extraction reaction apparatus including a reaction cassette 100, and the specific structure of the reaction cassette 100 refers to the above embodiment, and since the extraction reaction apparatus proposed by the present invention includes all aspects of all embodiments of the reaction cassette 100 described above, it has at least the same technical effects as the reaction cassette 100 described above, which are not described herein.
In some embodiments of the present invention, as shown in fig. 1, 8 and 9, the extraction reaction apparatus further includes a syringe 200, the syringe 200 is provided separately from the reaction cassette 100, and the syringe 200 can be inserted into the injection port 10a of the reaction cassette 100 to inject the liquid to be detected into the reaction cassette 100.
Referring mainly to fig. 8, in the present embodiment, the syringe 200 may include a syringe body 211, a protective cover 212, a push rod 213 and a lock catch 214; the protective cover 212 is arranged on the injection end of the injector body 211, the protective cover 212 can adopt a double-shot injection molding process to inject silica gel into plastic, and the protective cover 212 can be provided with sealing ribs for sealing the injection end of the injector body 211; the push rod 213 is inserted on the syringe body 211, and the push rod 213 may be provided in a circular rod shape or the like; the lock catch 214 is movably disposed on an end of the syringe body 211 and inserted into the push rod 213 to lock the push rod 213.
Compared with the conventional cross-shaped rod, the circular push rod 213 can reduce left and right shaking, and can ensure the pressure of the push rod 213.
In this embodiment, the lock catch 214 can prevent misoperation, and before the liquid in the syringe 200 is injected into the reaction box 100, the lock catch 214 needs to be taken out first, and then the push rod 213 is pushed to inject the mixed liquid into the reaction box 100.
In summary, the extraction reaction device can perform flow splitting, dilution, vibration, heating and amplification, and in the prior art, the operations are all completed by large-scale automatic equipment, the extraction reaction device can be independently formed into a detection system, a more convenient detection scheme is provided for each medical institution in society, and the scheme can be applied to the medical fields of antigen-antibody detection, virus detection, protein detection and the like. Through experimental verification and practical data analysis, the extraction reaction device can be fully used for mass production, and contributes to social medical treatment.
The extraction reaction device has the characteristics of simple structure, rapid detection, convenient operation, low risk, high tightness, high accuracy and the like, and meanwhile, the product is light in size, convenient to transport, turnover, store and recycle, and is beneficial to fully utilizing resources.
The foregoing description is only of the optional embodiments of the present invention, and is not intended to limit the scope of the invention, and all the equivalent structural changes made by the description of the present invention and the accompanying drawings or the direct/indirect application in other related technical fields are included in the scope of the invention.