技术领域Technical field
本发明涉及烟草香味液的制造方法、烟草香味液、以及香味抽吸器。The present invention relates to a manufacturing method of tobacco flavor liquid, a tobacco flavor liquid, and a flavor inhaler.
背景技术Background technique
已干燥的烟草植物的叶被用作香烟、加热式烟草等香味抽吸器的香味源，称为烟叶。烟叶中包含各种香味成分。烟叶可以直接作为香味抽吸器的香味源而使用，也可以从烟叶中提取烟草香味成分，将得到的烟草提取液作为香味抽吸器的香味源而使用。烟草提取液包含酵母等微生物，因此，在作为香味抽吸器的香味源而使用的情况下，希望将微生物去除。The dried leaves of the tobacco plant are used as the flavor source for cigarettes, heated tobacco and other flavored smokers, and are called tobacco leaves. Tobacco leaves contain various flavor components. Tobacco leaves can be directly used as the flavor source of the flavor inhaler, or tobacco flavor components can be extracted from the tobacco leaves and the obtained tobacco extract can be used as the flavor source of the flavor inhaler. Tobacco extract contains microorganisms such as yeast. Therefore, when it is used as a flavor source of a flavor inhaler, it is desirable to remove the microorganisms.
另外，已知通过使烟草提取液与酵母等微生物反应，可使烟草提取液中所含的香味成分增加(例如，专利文献1)。在该情况下，在将反应后的烟草提取液作为香味抽吸器的香味源而使用的情况下，也期望将微生物去除。Furthermore, it is known that the flavor components contained in the tobacco extract can be increased by reacting the tobacco extract with microorganisms such as yeast (for example, Patent Document 1). In this case, when the reacted tobacco extract liquid is used as a flavor source of a flavor inhaler, it is also desirable to remove microorganisms.
现有技术文献existing technical documents
专利文献patent documents
专利文献1：美国专利第4895175号Patent document 1: U.S. Patent No. 4895175
发明内容Contents of the invention
发明所要解决的问题Problems to be solved by inventions
本发明的目的在于提供在不损害烟草香味的情况下通过简便的方法将酵母等微生物从烟草提取液中去除的技术。The object of the present invention is to provide a technology for removing microorganisms such as yeast from tobacco extracts by a simple method without damaging the flavor of tobacco.
解决问题的方法way of solving the problem
根据一个方面，本发明提供一种烟草香味液的制造方法，该方法包括：According to one aspect, the present invention provides a method for manufacturing tobacco flavor liquid, which method includes:
用包含无机多孔体的材料对烟草提取液进行处理，将微生物从烟草提取液中去除。The tobacco extract is treated with a material containing an inorganic porous body to remove microorganisms from the tobacco extract.
根据另一方面，本发明提供一种烟草香味液，其是通过上述的方法而得到的。According to another aspect, the present invention provides a tobacco flavor liquid obtained by the above method.
根据又一方面，本发明提供一种香味抽吸器，其包含上述的烟草香味液。According to another aspect, the present invention provides a flavor inhaler, which contains the above-mentioned tobacco flavor liquid.
发明的效果Effect of invention
根据本发明，可以提供在不损害烟草香味的情况下通过简便的方法将酵母等微生物从烟草提取液中去除的技术。According to the present invention, it is possible to provide a technology for removing microorganisms such as yeast from tobacco extract liquid by a simple method without damaging the flavor of tobacco.
附图说明Description of the drawings
图1是示出烟草香味液的制造方法的一例的流程图。FIG. 1 is a flowchart showing an example of a method for producing tobacco flavor liquid.
图2是示出烟草香味液的制造方法的另一例的流程图。FIG. 2 is a flowchart showing another example of a method for producing tobacco flavor liquid.
图3是示出燃烧型香味抽吸器的一例的剖面图。FIG. 3 is a cross-sectional view showing an example of the combustion type fragrance inhaler.
图4是示出加热型香味抽吸器的一例的立体图。FIG. 4 is a perspective view showing an example of the heating type fragrance inhaler.
图5是示出烟草棒的内部结构的图。Fig. 5 is a diagram showing the internal structure of the tobacco rod.
图6是示出气溶胶生成装置的内部结构的图。FIG. 6 is a diagram showing the internal structure of the aerosol generating device.
具体实施方式Detailed ways
以下，对本发明详细地进行说明，但以下的说明的目的在于对本发明进行说明，并不意图限定本发明。The present invention will be described in detail below. However, the purpose of the following description is to explain the present invention and is not intended to limit the present invention.
＜1.烟草香味液的制造方法＞＜1. Manufacturing method of tobacco flavor liquid＞
烟草香味液的制造方法包括：用包含无机多孔体的材料对烟草提取液进行处理，将微生物从烟草提取液中去除。The manufacturing method of the tobacco flavor liquid includes: treating the tobacco extract liquid with a material containing an inorganic porous body to remove microorganisms from the tobacco extract liquid.
在该方法中，由于烟草提取液所包含的微生物被去除，因此，得到的烟草香味液与烟草提取液相比，具有量减少了的微生物。在本说明书中，“微生物”是指即将用包含无机多孔体的材料对烟草提取液进行处理之前烟草提取液中所含的任意的微生物，包含细菌、真菌。In this method, since microorganisms contained in the tobacco extract liquid are removed, the obtained tobacco flavor liquid has a reduced amount of microorganisms compared with the tobacco extract liquid. In this specification, "microorganisms" refer to any microorganisms contained in the tobacco extract immediately before the tobacco extract is treated with a material containing an inorganic porous body, including bacteria and fungi.
在第1实施方式中，烟草提取液是通过从烟草材料中用水性溶剂提取上述烟草材料所包含的水溶性成分而得到的烟草上清。第1实施方式的方法在以下的＜1-1.第1实施方式＞一栏中详细地进行说明。In the first embodiment, the tobacco extract liquid is a tobacco supernatant obtained by extracting the water-soluble components contained in the tobacco material from the tobacco material with an aqueous solvent. The method of the first embodiment will be described in detail in the column <1-1. First Embodiment> below.
在第2实施方式中，烟草提取液是通过包括以下的工序(a)～(c)的方法而得到的有用成分溶出液：In the second embodiment, the tobacco extract liquid is a useful component eluate obtained by a method including the following steps (a) to (c):
(a)用水性溶剂从烟草材料中提取上述烟草材料所包含的水溶性成分，得到烟草上清；(a) extracting the water-soluble components contained in the tobacco material from the tobacco material with a water-based solvent to obtain the tobacco supernatant;
(b)在上述烟草上清中培养酵母，得到含酵母培养液；以及(b) Cultivate yeast in the above-mentioned tobacco supernatant to obtain a yeast-containing culture solution; and
(c)将上述含酵母培养液与包含有机溶剂的溶出溶剂混合，使上述酵母的菌体内包含的有用成分从得到的混合物所含有的上述酵母溶出至上述混合物的液体部分，得到有用成分溶出液。第2实施方式的方法在以下的＜1-2.第2实施方式＞一栏中详细地进行说明。(c) Mixing the above-mentioned yeast-containing culture liquid with an elution solvent containing an organic solvent to dissolve the useful components contained in the cells of the above-mentioned yeast from the above-mentioned yeast contained in the obtained mixture to the liquid part of the above-mentioned mixture, thereby obtaining a useful component eluate . The method of the second embodiment will be described in detail in the column <1-2. Second Embodiment> below.
＜1-1.第1实施方式＞<1-1. First Embodiment>
根据第1实施方式，烟草香味液的制造方法包括：According to the first embodiment, a method for manufacturing tobacco flavor liquid includes:
(S1)用水性溶剂从烟草材料中提取上述烟草材料所包含的水溶性成分，得到烟草上清；和(S1) Extract the water-soluble components contained in the tobacco material from the tobacco material with a water-based solvent to obtain the tobacco supernatant; and
(S4)用包含无机多孔体的材料对上述烟草上清进行处理，得到无微生物的烟草上清。(S4) The above-mentioned tobacco supernatant is treated with a material containing an inorganic porous body to obtain a microorganism-free tobacco supernatant.
将第1实施方式的方法示于图1。参照图1，按照(S1)及(S4)的工序顺序对第1实施方式的方法进行说明。可以将通过第1实施方式的方法得到的“无微生物的烟草上清”用作“烟草香味液”。The method of the first embodiment is shown in Fig. 1 . Referring to FIG. 1 , the method of the first embodiment will be described in accordance with the process sequence of (S1) and (S4). The "microorganism-free tobacco supernatant" obtained by the method of the first embodiment can be used as the "tobacco flavor liquid".
[提取工序(S1)][Extraction process (S1)]
在提取工序(S1)中，用水性溶剂从烟草材料中提取烟草材料所包含的水溶性成分，得到烟草上清。在提取工序(S1)中，在得到烟草上清的同时也得到烟草残渣(参照图1)。In the extraction step (S1), water-soluble components contained in the tobacco material are extracted from the tobacco material with an aqueous solvent to obtain a tobacco supernatant. In the extraction step (S1), tobacco residue is also obtained along with the tobacco supernatant (see Fig. 1).
烟草材料可以使用准备好配合于燃烧型或加热型的香味抽吸器等烟草制品中的烟丝。“准备好配合于烟草制品中的烟丝”是指，经过由农户进行的干燥工序、随后在原料工厂中的1年至几年的长期熟化工序、以及随后在制造工厂中的混合及切丝等各种加工处理，从而准备好配合于烟草制品中的烟丝。As the tobacco material, shredded tobacco prepared for use in tobacco products such as combustion type or heating type flavor inhalers can be used. "Shredded tobacco that is ready to be blended into tobacco products" refers to the drying process performed by farmers, followed by a long-term aging process of one to several years in a raw material factory, and then mixed and shredded in a manufacturing factory. Various processing treatments to prepare cut tobacco for use in tobacco products.
烟丝是烟叶切丝物。烟丝可以为去筋烟叶的丝、叶梗的丝、再生烟草(即，将工厂的操作工序中产生的叶屑、丝屑、叶梗屑、细粉等加工成能够再使用的形状而成的烟草材料)的丝、或它们的混合物中的任意材料。为了提高提取效率，可以将烟丝粉碎，并将得到的粉碎物用于提取。Tobacco is shredded tobacco leaves. Shredded tobacco can be shredded tobacco leaves, shredded leaf stems, or recycled tobacco (that is, leaf scraps, shredded silk scraps, leaf stem scraps, fine powder, etc. generated during factory operations) are processed into shapes that can be reused. Tobacco material) filaments, or any material in their mixtures. In order to improve the extraction efficiency, shredded tobacco can be pulverized and the resulting pulverized material can be used for extraction.
烟丝可以使用任意品种的烟丝，例如可以使用黄色种、白肋烟种、东方烟草种等的烟丝。烟丝可以使用单一品种，也可以使用不同品种的混合物。Any type of cut tobacco can be used, for example, cut tobacco of yellow type, burley type, oriental type, etc. can be used. Cut tobacco can be of a single variety or a mixture of different varieties.
作为水性溶剂，可以使用水或含水乙醇。作为含水乙醇，例如，可以使用乙醇与水的体积比1:1的混合物。水性溶剂一般为水，优选为室温(例如，约20℃)～70℃的水。相对于烟草材料，水性溶剂例如可以以500～5000质量％的量使用。As the aqueous solvent, water or aqueous ethanol can be used. As the aqueous ethanol, for example, a mixture of ethanol and water with a volume ratio of 1:1 can be used. The aqueous solvent is generally water, preferably water at room temperature (for example, about 20°C) to 70°C. The aqueous solvent can be used in an amount of, for example, 500 to 5000% by mass relative to the tobacco material.
提取例如可以通过将烟草材料在40～60℃的温水中浸渍30～180分钟、或者将烟草材料在40～60℃的温水中振荡(例如200rpm)30～180分钟而进行。Extraction can be performed, for example, by immersing the tobacco material in warm water of 40 to 60° C. for 30 to 180 minutes, or by shaking the tobacco material in warm water of 40 to 60° C. (for example, 200 rpm) for 30 to 180 minutes.
另外，提取可以通过重复进行多次提取操作而进行。具体而言，可以通过用水性溶剂从烟草材料中提取烟草材料所包含的水溶性成分，然后，将得到的烟草残渣加入至新的水性溶剂中，进行第2次提取操作，进一步根据需要重复进行利用新的水性溶剂的提取操作，从而进行提取。In addition, extraction can be performed by repeating the extraction operation multiple times. Specifically, the water-soluble components contained in the tobacco material can be extracted from the tobacco material by using an aqueous solvent, and then the obtained tobacco residue is added to a new aqueous solvent to perform a second extraction operation, and the operation is repeated as necessary. Extraction is performed using new aqueous solvent extraction operations.
通过提取，可得到烟草残渣与烟草上清的混合物。烟草上清含有烟草材料所包含的水溶性成分。作为“烟草材料所包含的水溶性成分”，例如可举出：成为酵母等微生物的营养源的成分(例如，糖类、氨基酸、蛋白质、营养盐类)、对烟草香味有贡献的成分(例如，有机酸、叶面树脂、萜类、多酚类)等。Through extraction, a mixture of tobacco residue and tobacco supernatant can be obtained. The tobacco supernatant contains water-soluble components contained in the tobacco material. Examples of "water-soluble components contained in tobacco materials" include components that serve as nutrient sources for microorganisms such as yeast (e.g., sugars, amino acids, proteins, nutrient salts), and components that contribute to the flavor of tobacco (e.g. , organic acids, leaf resins, terpenes, polyphenols), etc.
在提取之后，将烟草残渣与烟草上清分离，将烟草上清作为用于得到烟草香味液的原料而使用。另一方面，可以将烟草残渣与最终得到的烟草香味液(在该实施方式中为“无微生物的烟草上清”)混合，对得到的混合物进行适当加工，用于制作烟草填充材料。例如，可以将烟草残渣与最终得到的烟草香味液混合，用于由得到的混合物制作片状烟草等烟草成形体。或者，可以将烟草残渣与最终得到的烟草香味液混合，将得到的混合物干燥并粉碎，用于制作烟草粉末。After the extraction, the tobacco residue and the tobacco supernatant are separated, and the tobacco supernatant is used as a raw material for obtaining the tobacco flavor liquid. On the other hand, the tobacco residue can be mixed with the finally obtained tobacco flavor liquid ("microorganism-free tobacco supernatant" in this embodiment), and the resulting mixture can be appropriately processed and used to prepare tobacco filler materials. For example, the tobacco residue can be mixed with the finally obtained tobacco flavor liquid, and the resulting mixture can be used to produce tobacco molded products such as sheet tobacco. Alternatively, the tobacco residue may be mixed with the finally obtained tobacco flavor liquid, and the resulting mixture may be dried and pulverized to produce tobacco powder.
[处理工序(S4)][Processing step (S4)]
在处理工序(S4)中，用包含无机多孔体的材料对烟草上清进行处理，将微生物从烟草上清中去除。由此，得到无微生物的烟草上清作为烟草香味液。In the treatment step (S4), the tobacco supernatant is treated with a material containing an inorganic porous body to remove microorganisms from the tobacco supernatant. Thus, a microorganism-free tobacco supernatant was obtained as a tobacco flavor liquid.
无机多孔体例如为硅藻土或沸石。硅藻土为由硅藻的壳的化石形成的堆积物，包含二氧化硅作为主成分。沸石为微观多孔性的结晶性含水铝硅酸盐，可以是天然沸石，也可以是合成沸石。The inorganic porous body is, for example, diatomaceous earth or zeolite. Diatomaceous earth is a deposit formed from the fossilized shells of diatoms and contains silica as a main component. Zeolite is a microporous crystalline hydrous aluminosilicate, which can be a natural zeolite or a synthetic zeolite.
通过处理工序(S4)被去除的微生物是烟草上清中所含的微生物，例如可举出酵母、霉菌、微细藻类等。由于在后述的实施例1中证实了烟草香味液的澄清性，因此可以认为，在处理工序(S4)中，能够将烟草上清中所含的所有微生物去除，而且可以将除微生物以外的微粒、例如1μm以上的尺寸的微粒也去除。The microorganisms removed in the treatment step (S4) are microorganisms contained in the tobacco supernatant, and examples thereof include yeast, mold, and microalgae. Since the clarity of the tobacco flavor liquid was confirmed in Example 1 described below, it is considered that in the treatment step (S4), all microorganisms contained in the tobacco supernatant can be removed, and all microorganisms other than microorganisms can be removed. Microparticles, for example, microparticles having a size of 1 μm or more are also removed.
烟草香味液中的微生物通过代谢而使烟草香味液的组成发生变化，成为品质变差的原因，因此，优选烟草香味液不含微生物。另外，在将烟草香味液应用于电子烟草(即，通过加热器对烟草香味液进行加热、使其蒸发/气化而供使用者抽吸的吸烟器具)的情况下，烟草香味液中的微生物会成为加热器的焦糊的原因，因此，优选烟草香味液不含微生物。Microorganisms in the tobacco flavor liquid change the composition of the tobacco flavor liquid through metabolism, causing quality deterioration. Therefore, it is preferable that the tobacco flavor liquid does not contain microorganisms. In addition, when the tobacco flavor liquid is applied to electronic tobacco (that is, a smoking device that heats the tobacco flavor liquid with a heater to evaporate/vaporize it for the user to smoke), microorganisms in the tobacco flavor liquid This may cause burning of the heater, so it is preferable that the tobacco flavor liquid does not contain microorganisms.
处理工序(S4)可以通过使烟草上清从包含无机多孔体的材料中通过而进行，也可以通过在烟草上清中添加包含无机多孔体的材料、然后将包含无机多孔体的材料从烟草上清中去除而进行。The treatment step (S4) may be performed by passing the tobacco supernatant through a material containing an inorganic porous body, or by adding a material containing an inorganic porous body to the tobacco supernatant, and then removing the material containing the inorganic porous body from the tobacco. It is carried out by removing it from the Qing Dynasty.
在优选的方式中，处理工序(S4)可以通过使烟草上清通过包含无机多孔体的层而进行。包含无机多孔体的层例如是包含硅藻土的层或包含沸石的层。“包含无机多孔体的层”可以是仅由无机多孔体构成的层，也可以是包含作为主成分的无机多孔体和作为追加成分的添加剂的层。作为添加剂，例如可举出：成形助剂、粘合剂等。In a preferred embodiment, the treatment step (S4) can be performed by passing the tobacco supernatant through a layer containing an inorganic porous body. The layer containing an inorganic porous body is, for example, a layer containing diatomaceous earth or a layer containing zeolite. The "layer containing an inorganic porous body" may be a layer composed only of an inorganic porous body, or may be a layer containing an inorganic porous body as a main component and an additive as an additional component. Examples of additives include molding aids, binders, and the like.
在一个方式中，“包含无机多孔体的层”可以由包含无机多孔体的成形体构成。即，处理工序(S4)可以通过使烟草上清通过包含无机多孔体的成形体而进行。包含无机多孔体的成形体例如为包含硅藻土的成形体或包含沸石的成形体。“包含无机多孔体的成形体”可以是仅由无机多孔体构成的成形体，也可以是包含作为主成分的无机多孔体和作为追加成分的添加剂的成形体。作为添加剂，例如可举出：成形助剂、粘合剂等。更具体而言，“包含无机多孔体的成形体”可以是包含无机多孔体的烧成成形体，也可以是包含无机多孔体的粒子的压缩成形体。In one aspect, the "layer containing an inorganic porous body" may be composed of a shaped body containing an inorganic porous body. That is, the treatment step (S4) can be performed by passing the tobacco supernatant through a molded body containing an inorganic porous body. The molded article containing the inorganic porous body is, for example, a molded article containing diatomite or a molded article containing zeolite. The “molded article containing an inorganic porous body” may be a molded article composed only of an inorganic porous body, or may be a molded article containing an inorganic porous body as a main component and an additive as an additional component. Examples of additives include molding aids, binders, and the like. More specifically, the "formed body containing an inorganic porous body" may be a fired molded body containing an inorganic porous body, or a compression molded body containing particles of an inorganic porous body.
包含硅藻土的成形体例如可以使用CELITE(注册商标)(GL Sciences公司)、SUPRAdisc(Nihon Pall公司)。Examples of molded articles containing diatomaceous earth include CELITE (registered trademark) (GL Sciences) and SUPRAdisc (Nihon Pall).
或者，在其它方式中，“包含无机多孔体的层”可以由包含无机多孔体的粒子的集合体构成。即，处理工序(S4)可以通过使烟草上清通过包含无机多孔体的粒子的集合体而进行。包含无机多孔体的粒子例如为包含硅藻土的粒子或包含沸石的粒子。“包含无机多孔体的粒子”可以是仅由无机多孔体构成的粒子，也可以是包含主成分的无机多孔体和作为追加成分的添加剂的粒子。作为添加剂，例如可举出：成形助剂、粘合剂等。Alternatively, in another aspect, the "layer containing an inorganic porous body" may be composed of an aggregate of particles containing an inorganic porous body. That is, the treatment step (S4) can be performed by passing the tobacco supernatant through an aggregate of particles containing an inorganic porous body. Particles containing an inorganic porous body are, for example, particles containing diatomaceous earth or particles containing zeolite. "Particles containing an inorganic porous body" may be particles composed only of an inorganic porous body, or may be particles containing an inorganic porous body as a main component and an additive as an additional component. Examples of additives include molding aids, binders, and the like.
“包含无机多孔体的粒子”例如具有＜400μm的粒度、一般为10～400μm的粒度、优选为＜100μm的粒度、更优选为10～100μm的粒度、进一步优选为10～80μm的粒度、进一步优选为30～75μm的粒度。＜Xμm的粒度表示粒径小于Xμm。即，＜Xμm的粒度是指通过了Xμm的网眼的筛的粒子的粒度。另外，Y～Zμm的粒度表示粒径为Yμm以上且小于Zμm。即，Y～Zμm的粒度是指通过了Zμm的网眼的筛但没有通过Yμm的网眼的筛的粒子的粒度。在本说明书中，粒度是指通过筛分粒度测定法(JIS Z 8815:1994)测得的值。"Particles containing an inorganic porous body" have, for example, a particle size of <400 μm, generally a particle size of 10 to 400 μm, preferably a particle size of <100 μm, more preferably a particle size of 10 to 100 μm, still more preferably a particle size of 10 to 80 μm, still more preferably The particle size is 30~75μm. A particle size of <X μm means that the particle size is smaller than X μm. That is, the particle size of < In addition, the particle size of Y to Zμm means that the particle diameter is Yμm or more and smaller than Zμm. That is, the particle size of Y to Z μm refers to the particle size of particles that passed through a sieve with a mesh size of Z μm but did not pass through a sieve with a mesh size of Y μm. In this specification, the particle size refers to the value measured by the sieve granulometry method (JIS Z 8815:1994).
包含硅藻土的粒子例如可以使用以K-solute的商品名市售的硅藻土的粉碎品(粒度：＜354μm)(GL Sciences公司)。包含沸石的粒子例如可以使用合成沸石粉末(粒度：＜75μm)(富士胶片和光纯药株式会社)。As the particles containing diatomite, for example, a crushed product of diatomite (particle size: <354 μm) commercially available under the trade name K-solute (GL Sciences) can be used. As the particles containing zeolite, for example, synthetic zeolite powder (particle size: <75 μm) (Fuji Film Wako Pure Chemical Industries, Ltd.) can be used.
在更优选的方式中，处理工序(S4)可以通过使烟草上清通过填充有包含无机多孔体的粒子的集合体的柱而进行。如以上所说明的那样，“包含无机多孔体的粒子”例如为包含硅藻土的粒子或包含沸石的粒子。柱例如可以使用具有内径11～100mm及长度100～1000mm的尺寸的柱。烟草上清例如可以利用重力落下而从柱中通过。In a more preferred embodiment, the treatment step (S4) can be performed by passing the tobacco supernatant through a column filled with an aggregate of particles containing an inorganic porous body. As described above, "particles containing an inorganic porous body" are, for example, particles containing diatomaceous earth or particles containing zeolite. For example, a column having an inner diameter of 11 to 100 mm and a length of 100 to 1000 mm can be used. The tobacco supernatant can, for example, fall by gravity and pass through the column.
＜1-2.第2实施方式＞<1-2. Second Embodiment>
在第2实施方式中，使烟草上清与酵母进行反应，使烟草上清中所含的香味成分增加后，将微生物从增加了香味成分后的烟草上清中去除。即，根据第2实施方式，烟草香味液的制造方法包括：In the second embodiment, the tobacco supernatant is reacted with yeast to increase the flavor components contained in the tobacco supernatant, and then microorganisms are removed from the tobacco supernatant to which the flavor components are added. That is, according to the second embodiment, the manufacturing method of tobacco flavor liquid includes:
(S1)用水性溶剂从烟草材料中提取上述烟草材料所包含的水溶性成分，得到烟草上清；(S1) Extract the water-soluble components contained in the tobacco material from the tobacco material with a water-based solvent to obtain the tobacco supernatant;
(S2)在上述烟草上清中培养酵母，得到含酵母培养液；(S2) Cultivate yeast in the above-mentioned tobacco supernatant to obtain a yeast-containing culture solution;
(S3)将上述含酵母培养液与包含有机溶剂的溶出溶剂混合，使上述酵母的菌体内包含的有用成分从得到的混合物所含有的上述酵母溶出至上述混合物的液体部分，得到有用成分溶出液；以及(S3) Mix the above-mentioned yeast-containing culture liquid with an elution solvent containing an organic solvent, and dissolve the useful components contained in the cells of the above-mentioned yeast from the above-mentioned yeast contained in the obtained mixture into the liquid part of the above-mentioned mixture, thereby obtaining a useful component eluate. ;as well as
(S4)用包含无机多孔体的材料对上述有用成分溶出液进行处理，得到无微生物的有用成分溶出液。(S4) The above-mentioned useful component eluate is treated with a material containing an inorganic porous body to obtain a microorganism-free useful component eluate.
将第2实施方式的方法示于图2。参照图2，按照(S1)、(S2)、(S3)及(S4)的工序顺序对第2实施方式的方法进行说明。在以下的说明中，省略关于与第1实施方式的方法重复的部分的说明，以与第1实施方式的方法不同的部分为中心进行说明。可以将通过第2实施方式的方法得到的“无微生物的有用成分溶出液”用作“烟草香味液”。The method of the second embodiment is shown in Fig. 2 . Referring to FIG. 2 , the method of the second embodiment will be described in accordance with the process sequence of (S1), (S2), (S3), and (S4). In the following description, description of parts that are overlapping with the method of the first embodiment will be omitted, and description will be focused on parts that are different from the method of the first embodiment. The "microorganism-free useful component eluate" obtained by the method of the second embodiment can be used as the "tobacco flavor liquid".
[提取工序(S1)][Extraction process (S1)]
在提取工序(S1)中，用水性溶剂从烟草材料中提取烟草材料所包含的水溶性成分，得到烟草上清。在提取工序(S1)中，在得到烟草上清的同时，还得到烟草残渣(参照图2)。提取工序(S1)可以与在第1实施方式的方法中说明的提取工序(S1)同样地实施。In the extraction step (S1), water-soluble components contained in the tobacco material are extracted from the tobacco material with an aqueous solvent to obtain a tobacco supernatant. In the extraction step (S1), not only the tobacco supernatant is obtained, but also the tobacco residue is obtained (see Fig. 2). The extraction step (S1) can be implemented in the same manner as the extraction step (S1) described in the method of the first embodiment.
[培养工序(S2)][Cultivation process (S2)]
在培养工序(S2)中，在通过提取工序(S1)得到的烟草上清中对酵母进行培养，得到含酵母培养液(参照图2)。In the culture step (S2), yeast is cultured in the tobacco supernatant obtained in the extraction step (S1) to obtain a yeast-containing culture liquid (see Fig. 2).
酵母只要能够在烟草上清中进行培养时生产有用成分即可，可以使用任意种类的酵母。Any type of yeast can be used as long as it can produce useful components when cultured in tobacco supernatant.
在本说明书中，“有用成分”是指对于最终得到的烟草香味液而言有用的成分。有用成分例如可以是对烟草香味液的香味有贡献的成分(以下称为香味贡献成分)，也可以是使烟草香味液着色的成分(以下称为着色成分)，还可以是防止烟草香味液的腐坏、发酵的成分(以下称为防腐成分)。In this specification, "useful components" refer to components that are useful for the finally obtained tobacco flavor liquid. The useful component may be, for example, a component that contributes to the flavor of the tobacco flavor liquid (hereinafter referred to as a flavor contributing component), a component that colors the tobacco flavor liquid (hereinafter referred to as a coloring component), or a component that prevents the tobacco flavor liquid from Putrefactive and fermented components (hereinafter referred to as preservative components).
有用成分优选为香味贡献成分。“香味贡献成分”可以是释放出香味的香味成分，也可以是通过香味抽吸器进行加热或燃烧时转化成香味成分的前体。The useful ingredient is preferably a flavor-contributing ingredient. "Fragrance contributing components" may be fragrance components that release fragrance, or may be precursors that are converted into fragrance components when heated or burned by a fragrance inhaler.
作为香味贡献成分，例如可举出：类胡萝卜素、脂肪酸、中性脂肪(即，脂肪酸的甘油酯)、乙酸酯、脂肪酸酯、有机酸、高级醇(例如，碳原子数8～22的醇)等。作为着色成分，例如可举出类胡萝卜素。作为防腐成分，例如可举出乳酸、脂肪酸苷、苯甲酸等。Examples of aroma-contributing components include carotenoids, fatty acids, neutral fats (that is, glyceryl esters of fatty acids), acetates, fatty acid esters, organic acids, and higher alcohols (for example, those with 8 to 22 carbon atoms). of alcohol) etc. Examples of coloring components include carotenoids. Examples of antiseptic components include lactic acid, fatty acid glycosides, benzoic acid, and the like.
因此，在本发明的方法中可以使用已知可生产上述的有用成分的酵母。Therefore, yeasts known to produce the above-mentioned useful ingredients can be used in the method of the present invention.
作为生产类胡萝卜素的酵母，可举出Rhodotorula(红酵母)属的酵母、例如Rhodotorula alborubescens(浅红红酵母)、Rhodotorula araucariae(南美杉红酵母)、Rhodotorula babjevae(巴布伊娃红酵母)、Rhodotorula dairenensis、Rhodotoruladiobovata(双倒卵形红酵母)、Rhodotorula evergladensis、Rhodotorula glutinis(粘红酵母)、Rhodotorula graminis(禾本红酵母)、Rhodotorula kratochvilovae(克拉托奇维洛娃红酵母)、Rhodotorula mucilaginosa(胶红酵母)、Rhodotorula ngohengohe、Rhodotorula pacifica(太平洋红酵母)、Rhodotorula paludigena(沼泽红酵母)、Rhodotorula sinensis、Rhodotorula sphaerocarpa(球红酵母)、Rhodotorulataiwanensis、Rhodotorula toruloides(圆红酵母)；Xanthophyllomyces(法夫酵母)属的酵母、例如Xanthophyllomyces australis、Xanthophyllomyces rhodozyma、Xanthophyllomyces tasmanica。Examples of yeasts that produce carotenoids include yeasts of the genus Rhodotorula, such as Rhodotorula alborubescens, Rhodotorula araucariae, Rhodotorula babjevae, Rhodotorula dairenensis, Rhodotoruladiobovata, Rhodotorula evergladensis, Rhodotorula glutinis, Rhodotorula graminis, Rhodotorula kratochvilovae, Rhodotorula mucilaginosa ), Rhodotorula ngohengohe, Rhodotorula pacifica, Rhodotorula paludigena, Rhodotorula sinensis, Rhodotorula sphaerocarpa, Rhodotorulataiwanensis, Rhodotorula toruloides; Xanthophyllomyces Yeast, such as Xanthophyllomyces australis, Xanthophyllomyces rhodozyma, Xanthophyllomyces tasmanica.
作为生产脂肪酸的酵母，可举出Yarrowia(耶氏酵母)属的酵母、例如Yarrowiaalimentaria、Yarrowia bubula(布布拉耶氏酵母)、Yarrowia deformans(畸形耶氏酵母)、Yarrowia divulgata、Yarrowia galli、Yarrowia hollandica、Yarrowia keelungensis、Yarrowia lipolytica(耶氏解脂酵母)、Yarrowia osloensis、Yarrowia parophoni、Yarrowia phangngaensis、Yarrowia porcina(猪耶氏酵母)、Yarrowia yakushimensis(耶氏克鲁维酵母)；Lipomyces(油脂酵母)属的酵母、例如Lipomyces anomalus、Lipomycesarxii、Lipomyces chichibuensis、Lipomyces doorenjongii、Lipomyces japonicus、Lipomyces kockii、Lipomyces kononenkoae(橘林油脂酵母)、Lipomyces lipofer(产油油脂酵母)、Lipomyces mesembrius、Lipomyces okinawensis、Lipomyces oligophaga、Lipomyces orientalis、Lipomyces smithiae、Lipomyces spencermartinsiae、Lipomycesstarkeyi(斯达油脂酵母)。Examples of yeasts that produce fatty acids include yeasts of the genus Yarrowia, such as Yarrowia alimentaria, Yarrowia bubula, Yarrowia deformans, Yarrowia divulgata, Yarrowia galli, and Yarrowia hollandica. , Yarrowia keelungensis, Yarrowia lipolytica (Yarrowia lipolytica), Yarrowia osloensis, Yarrowia parophoni, Yarrowia phangngaensis, Yarrowia porcina (Yarrowia pig), Yarrowia yakushimensis (Kluyveromyces yarrowi); Lipomyces (oil yeast) genus Yeast, such as Lipomyces anomalus, Lipomyces arxii, Lipomyces chichibuensis, Lipomyces doorenjongii, Lipomyces japonicus, Lipomyces kockii, Lipomyces kononenkoae (orange oil yeast), Lipomyces lipofer (oil-producing oil yeast), Lipomyces mesembrius, Lipomyces okinawensis, Lipomyces oligophaga, Lipomyces orientalis , Lipomyces smithiae, Lipomyces spencermartinsiae, Lipomycesstarkeyi (star grease yeast).
作为生产乙酸酯、脂肪酸酯、或高级醇的酵母，可举出Saccharomyces(酵母菌)属的酵母、例如Saccharomyces cerevisiae(酿酒酵母)、Saccharomyces paradoxus(奇异酵母)、Saccharomyces bayanus(贝酵母)、Saccharomyces uvarum(葡萄汁酵母)、Saccharomyces arboricola；Cyberlindnera(塞伯林德纳氏酵母)属的酵母、例如Cyberlindnera jadinii(杰丁塞伯林德纳氏酵母)、Cyberlindnera saturnus(土星形塞伯林德纳氏酵母)、Cyberlindnera fabianii(费比恩塞伯林德纳氏酵母)、Cyberlindnerasuaveolens、Cyberlindnera americana、Cyberlindnera xylosilytyca；Wickerhamomyces(威克汉姆酵母)属的酵母、例如Wickerhamomyces anomalus(异常威克汉姆酵母)、Wickerhamomyces ciferri(威克汉姆西弗酵母)、Wickerhamomyces canadensis(加拿大威克汉姆酵母)。Examples of yeasts that produce acetate, fatty acid esters, or higher alcohols include yeasts of the genus Saccharomyces, such as Saccharomyces cerevisiae, Saccharomyces paradoxus, and Saccharomyces bayanus. Saccharomyces uvarum (grape juice yeast), Saccharomyces arboricola; Yeasts of the genus Cyberlindnera (Cyberlindnera), such as Cyberlindnera jadinii (Jadin), Cyberlindnera saturnus (Saturn-shaped Cyberlindnera) Saccharomyces cerevisiae), Cyberlindnera fabianii, Cyberlindnerasuaveolens, Cyberlindnera americana, Cyberlindnera xylosilytyca; Yeasts of the genus Wickerhamomyces, such as Wickerhamomyces anomalus , Wickerhamomyces ciferri (Wickerhamomyces yeast), Wickerhamomyces canadensis (Canadian Wickerham yeast).
可以在烟草上清中培养1种酵母，也可以在烟草上清中培养2种以上的酵母。另外，酵母也可以是以增加有用成分的生产量的方式进行了基因上的修饰后的基因重组酵母。One type of yeast can be cultured in the tobacco supernatant, or two or more types of yeast can be cultured in the tobacco supernatant. In addition, the yeast may be genetically recombinant yeast that has been genetically modified to increase the production amount of useful components.
酵母的培养条件没有特别限定，可以适当选择适于所使用的酵母的生长及有用成分的生产的条件。可以在培养之前，在烟草上清中例如以10～108细胞/mL的浓度添加酵母。培养例如可以在10～40℃下进行例如5～168小时。The yeast culture conditions are not particularly limited, and conditions suitable for the growth of the yeast used and the production of useful components can be appropriately selected. Yeast may be added to the tobacco supernatant at a concentration of, for example, 10 to 108 cells/mL before culturing. The culture can be performed, for example, at 10 to 40° C. for 5 to 168 hours.
烟草上清中包含成为酵母的营养源的成分、成为有用成分的原料的成分，可以提供适于酵母的生长及有用成分的生产的环境。因此，不需要在烟草上清中添加追加的成分。但是，本发明的方法并不排除在烟草上清中添加追加的成分。The tobacco supernatant contains components that serve as nutrient sources for yeast and components that serve as raw materials for useful components, and can provide an environment suitable for the growth of yeast and the production of useful components. Therefore, there is no need to add additional components to the tobacco supernatant. However, the method of the present invention does not exclude the addition of additional components to the tobacco supernatant.
在本说明书中，将在烟草上清中对酵母进行培养后得到的酵母与烟草上清的混合物称为“含酵母培养液”。含酵母培养液与“培养前的酵母与烟草上清的混合物”相比，酵母所生产的有用成分的量增加。另外，含酵母培养液与“培养前的酵母与烟草上清的混合物”相比，用于酵母的生长、有用成分的生产所消耗的物质的量减少。In this specification, a mixture of yeast and tobacco supernatant obtained by culturing yeast in tobacco supernatant is called "yeast-containing culture solution". The yeast-containing culture liquid increases the amount of useful components produced by the yeast compared to the "mixture of yeast and tobacco supernatant before culture". In addition, the amount of substances consumed for the growth of yeast and the production of useful ingredients is reduced in the yeast-containing culture solution compared with the "mixture of yeast and tobacco supernatant before culture".
[溶出工序(S3)][Dissolution process (S3)]
在溶出工序(S3)中，将培养工序(S2)中得到的含酵母培养液与包含有机溶剂的溶出溶剂混合，使酵母的菌体内包含的有用成分从得到的混合物所含有的酵母溶出至混合物的液体部分。由此，可得到有用成分溶出液(参照图2)。In the elution step (S3), the yeast-containing culture liquid obtained in the culture step (S2) is mixed with an elution solvent containing an organic solvent, and useful components contained in the yeast cells are eluted from the yeast contained in the obtained mixture into the mixture. liquid part. Thereby, a useful component eluate can be obtained (refer to FIG. 2).
在本说明书中，将结束溶出工序后得到的含有酵母和溶出溶剂的混合物称为“有用成分溶出液”。In this specification, the mixture containing yeast and the dissolution solvent obtained after completing the dissolution process is called "effective component eluate".
作为溶出溶剂，可使用包含有机溶剂的溶出溶剂。溶出溶剂可以是有机溶剂其本身，也可以是有机溶剂与水的混合物。溶出溶剂优选为包含与水具有混和性的有机溶剂的溶出溶剂，更优选为包含与水具有混和性的醇的溶出溶剂。即，优选的溶出溶剂为与水具有混和性的醇、或其含水醇。As the elution solvent, an elution solvent containing an organic solvent can be used. The dissolution solvent may be the organic solvent itself or a mixture of the organic solvent and water. The elution solvent is preferably an elution solvent containing an organic solvent that is miscible with water, and more preferably is an elution solvent that contains an alcohol that is miscible with water. That is, a preferred dissolution solvent is an alcohol miscible with water, or a water-containing alcohol thereof.
溶出溶剂中包含的有机溶剂优选为具有10～14.5的SP值的有机溶剂，更优选为具有10～14.5的SP值的醇。作为溶出溶剂中包含的有机溶剂的例子，可举出乙醇、异丙醇、甲醇或丁醇。溶出溶剂中包含的有机溶剂更优选为乙醇或异丙醇，最优选为乙醇。即，最优选的溶出溶剂为乙醇或含水乙醇。The organic solvent contained in the elution solvent is preferably an organic solvent having an SP value of 10 to 14.5, and more preferably an alcohol having an SP value of 10 to 14.5. Examples of the organic solvent contained in the elution solvent include ethanol, isopropyl alcohol, methanol, or butanol. The organic solvent contained in the dissolution solvent is more preferably ethanol or isopropyl alcohol, and most preferably ethanol. That is, the most preferred dissolution solvent is ethanol or aqueous ethanol.
SP值是指希尔德布兰德(Hildebrand)溶解度参数的值。溶剂的SP值越高，表示与水的混和性越高。SP值在各种溶剂中是公知的，例如，乙醇的SP值为12.7、异丙醇的SP值为11.5，甲醇的SP值为14.5，丁醇的SP值为11.4。The SP value refers to the value of the Hildebrand solubility parameter. The higher the SP value of a solvent, the higher its miscibility with water. The SP value is well known in various solvents. For example, the SP value of ethanol is 12.7, isopropyl alcohol has an SP value of 11.5, methanol has an SP value of 14.5, and butanol has an SP value of 11.4.
溶出溶剂中包含的有机溶剂可以使用1种，也可以混合使用2种以上。The organic solvent contained in the dissolution solvent may be used alone, or two or more types may be mixed and used.
溶出溶剂可以以含酵母培养液与溶出溶剂的混合液中的有机溶剂的浓度例如达到50体积％以上、优选达到50～95体积％、更优选达到60～95体积％的量而添加于含酵母培养液。有机溶剂的浓度可以考虑有用成分的溶出效率而适当调整。在本说明书中，“含酵母培养液与溶出溶剂的混合液(mixed solution)”的用语是指含酵母培养液的液体部分(即，培养后的烟草上清)与溶出溶剂的混合液，不包含酵母。The dissolution solvent can be added to the yeast-containing liquid in an amount such that the concentration of the organic solvent in the mixed solution of the yeast-containing culture solution and the dissolution solvent reaches, for example, 50% by volume or more, preferably 50 to 95% by volume, and more preferably 60 to 95% by volume. Culture fluid. The concentration of the organic solvent can be appropriately adjusted taking into account the dissolution efficiency of the useful component. In this specification, the term "mixed solution containing yeast culture solution and dissolution solvent" refers to the mixed solution containing the liquid part of yeast culture solution (that is, the tobacco supernatant after culture) and the dissolution solvent. Contains yeast.
另外，相对于含酵母培养液，溶出溶剂例如可以以100～900体积％的量添加。溶出溶剂的添加量可以考虑有用成分的溶出效率而适当调整。In addition, the dissolution solvent may be added in an amount of, for example, 100 to 900% by volume relative to the yeast-containing culture solution. The amount of the dissolution solvent added can be appropriately adjusted in consideration of the dissolution efficiency of the useful component.
例如，在想要使用乙醇或含水乙醇作为溶出溶剂而使作为有用成分的脂肪酸溶出的情况下，乙醇可以以使含酵母培养液与溶出溶剂的混合液中的乙醇的浓度例如达到50体积％以上、优选达到50～90体积％、更优选达到60～90体积％、进一步优选达到60～80体积％、最优选达到70体积％的量而添加于含酵母培养液。For example, when ethanol or hydrous ethanol is used as the elution solvent to elute fatty acids as useful components, the concentration of ethanol in the mixed solution containing the yeast culture liquid and the elution solvent may be, for example, 50% by volume or more. , preferably 50 to 90 volume %, more preferably 60 to 90 volume %, further preferably 60 to 80 volume %, most preferably 70 volume %, and is added to the yeast-containing culture solution.
例如，在想要使用乙醇或含水乙醇作为溶出溶剂而使作为有用成分的类胡萝卜素溶出的情况下，乙醇可以以使含酵母培养液与溶出溶剂的混合液中的乙醇的浓度例如达到50体积％以上、优选达到50～90体积％、更优选达到60～90体积％、进一步优选达到70～90体积％、进一步优选达到80～90体积％、最优选达到90体积％的量而添加于含酵母培养液。For example, when ethanol or hydrous ethanol is used as the elution solvent to elute carotenoids as useful components, the ethanol may be used so that the concentration of ethanol in the mixed solution containing the yeast culture liquid and the elution solvent reaches, for example, 50 vol. % or more, preferably 50 to 90 volume %, more preferably 60 to 90 volume %, further preferably 70 to 90 volume %, further preferably 80 to 90 volume %, most preferably 90 volume %, and is added to the Yeast culture.
在溶出工序(S3)中，将含酵母培养液与溶出溶剂混合后，使酵母的菌体内包含的有用成分从得到的混合物所含有的酵母中溶出至混合物的液体部分。溶出可以通过一边根据需要对含酵母培养液与溶出溶剂的混合物进行加热一边搅拌给定期间而进行。In the dissolution step (S3), after the yeast-containing culture liquid and the dissolution solvent are mixed, the useful components contained in the cells of the yeast are eluted from the yeast contained in the obtained mixture into the liquid part of the mixture. Dissolution can be performed by stirring for a predetermined period while heating the mixture containing the yeast culture solution and the dissolution solvent as necessary.
从有用成分的溶出效率的观点考虑，优选一边对混合物进行搅拌一边进行溶出，但也可以将混合物静置并且进行溶出。在对混合物进行搅拌的情况下，搅拌速度例如可以设为60～300rpm。From the viewpoint of the dissolution efficiency of the useful component, it is preferable to perform dissolution while stirring the mixture, but the mixture may also be allowed to stand while dissolution is performed. When the mixture is stirred, the stirring speed can be, for example, 60 to 300 rpm.
另外，在通过加热促进有用成分的溶出的情况下，可以一边加热一边进行溶出，在无法观察到由加热带来的促进溶出的效果的情况下，可以不加热而在室温(例如15～25℃)下进行溶出。In addition, when the dissolution of useful components is accelerated by heating, the dissolution can be performed while heating. When the effect of promoting dissolution by heating cannot be observed, the dissolution can be performed at room temperature (for example, 15 to 25°C) without heating. ) for dissolution.
溶出温度及溶出时间可以考虑有用成分的溶出效率而适当调整。The dissolution temperature and dissolution time can be appropriately adjusted taking into account the dissolution efficiency of the useful ingredient.
例如，在想要使用乙醇或含水乙醇作为溶出溶剂而使作为有用成分的脂肪酸溶出的情况下，可以通过在室温(例如15～25℃)下将混合物搅拌5～60分钟来进行溶出。For example, when ethanol or hydrous ethanol is used as an elution solvent to elute fatty acids as useful components, elution can be performed by stirring the mixture at room temperature (for example, 15 to 25° C.) for 5 to 60 minutes.
例如，在想要使用乙醇或含水乙醇作为溶出溶剂而使作为有用成分的类胡萝卜素溶出的情况下，可以通过一边将混合物加热至80～95℃一边搅拌15～60分钟来进行溶出。For example, when ethanol or hydrous ethanol is used as an elution solvent to elute carotenoids as useful components, elution can be performed by stirring the mixture for 15 to 60 minutes while heating it to 80 to 95°C.
[处理工序(S4)][Processing step (S4)]
在处理工序(S4)中，用包含硅藻土的材料对溶出工序(S3)中得到的有用成分溶出液进行处理，将微生物从有用成分溶出液中去除。由此，得到无微生物的有用成分溶出液作为烟草香味液。处理工序(S4)可以与第1实施方式的方法中说明的处理工序(S4)同样地实施。In the treatment step (S4), the useful ingredient eluate obtained in the dissolution step (S3) is treated with a material containing diatomaceous earth to remove microorganisms from the useful ingredient eluate. Thus, a microorganism-free useful component eluate was obtained as a tobacco flavor liquid. The processing step (S4) can be implemented in the same manner as the processing step (S4) described in the method of the first embodiment.
通过处理工序(S4)被去除的微生物是培养工序(S2)中使用的酵母及烟草上清中所含的微生物。作为烟草上清中所含的微生物，例如可举出酵母、霉菌、微细藻类等。由于在后述的实施例3中证实了烟草香味液的澄清性，因此可以认为，在处理工序(S4)中，能够将培养工序(S2)中使用的酵母及烟草上清中所含的所有微生物去除，而且可以将微生物以外的微粒、例如1μm以上的尺寸的微粒也去除。The microorganisms removed in the treatment step (S4) are microorganisms contained in the yeast and tobacco supernatant used in the culture step (S2). Examples of microorganisms contained in the tobacco supernatant include yeast, mold, microalgae, and the like. Since the clarity of the tobacco flavor liquid was confirmed in Example 3 described below, it is considered that all the yeast used in the culture step (S2) and the tobacco supernatant can be removed in the treatment step (S4). Microorganisms are removed, and particles other than microorganisms, such as particles with a size of 1 μm or more, can also be removed.
第2实施方式的方法可以在得到了“无微生物的有用成分溶出液”之后进一步包括将有机溶剂从“无微生物的有用成分溶出液”中去除。有机溶剂的去除可以通过减压浓缩、常压浓缩、喷雾干燥等一般的方法来进行。The method of the second embodiment may further include removing the organic solvent from the "microorganism-free useful ingredient eluate" after obtaining the "microorganism-free useful ingredient eluate". The organic solvent can be removed by general methods such as concentration under reduced pressure, concentration under normal pressure, and spray drying.
＜1-3.效果＞＜1-3. Effect＞
根据本发明的方法，能够通过简便的方法将酵母等微生物从烟草提取液(在第1实施方式中为“烟草上清”，在第2实施方式中为“有用成分溶出液”)中去除而不损害烟草香味。由此，可以通过简便的方法制造微生物量减少了的烟草香味液(在第1实施方式中为“无微生物的烟草上清”，在第2实施方式中为“无微生物的有用成分溶出液”)而不损害烟草香味。According to the method of the present invention, microorganisms such as yeast can be removed from the tobacco extract ("tobacco supernatant" in the first embodiment and "effective component eluate" in the second embodiment) by a simple method. Does not damage tobacco flavor. This allows a simple method to produce a tobacco flavor liquid with a reduced amount of microorganisms ("microorganism-free tobacco supernatant" in the first embodiment, and "microorganism-free useful component eluate" in the second embodiment). ) without damaging the flavor of tobacco.
以下，对本发明的效果详细地进行说明。Hereinafter, the effects of the present invention will be described in detail.
为了将微生物从烟草提取液中去除，本发明的方法仅利用包含无机多孔体的材料进行处理即可，不需要进行精密过滤。对于精密过滤而言，不仅过滤需要花费时间，而且消除过滤膜的堵塞的等装置的维护也耗费劳力。本发明的方法即使在使烟草提取液通过包含无机多孔体的层的情况下，阻力也小，与利用精密过滤膜的处理相比，明显简便，在生产效率的方面优异。In order to remove microorganisms from tobacco extract liquid, the method of the present invention only needs to use materials containing inorganic porous bodies for treatment, and does not require precision filtration. For precision filtration, not only does filtration take time, but also the maintenance of devices such as removing clogging of the filter membrane is also labor-intensive. The method of the present invention has low resistance even when the tobacco extract liquid is passed through a layer containing an inorganic porous body, is significantly simpler than the treatment using a precision filtration membrane, and is excellent in production efficiency.
另外，本发明的方法能够以与利用精密过滤膜的处理同等程度地将微生物去除，因此，在微生物的去除效率的方面优异(参照后述的实施例1及3)。In addition, the method of the present invention can remove microorganisms to the same extent as the treatment using a precision filtration membrane, and therefore is excellent in microorganism removal efficiency (see Examples 1 and 3 described below).
另外，本发明的方法将微生物、与微生物相同尺寸的微粒去除，但是不会将烟草香味成分去除，因此，本发明的烟草香味液作为香味抽吸器的烟草香味源是优异的(参照后述的实施例4)。In addition, the method of the present invention removes microorganisms and particles of the same size as the microorganisms, but does not remove tobacco flavor components. Therefore, the tobacco flavor liquid of the present invention is excellent as a tobacco flavor source for a flavor inhaler (see below) Embodiment 4).
尤其是，第2实施方式的方法在烟草上清中对酵母进行培养，然后，使酵母的菌体内包含的有用成分溶出至烟草上清，将酵母从得到的有用成分溶出液中去除。因此，根据第2实施方式的方法，能够制造包含大量的有用成分、并且减少了微生物量的烟草香味液。In particular, the method of the second embodiment cultivates yeast in tobacco supernatant, then dissolves the useful components contained in the yeast cells into the tobacco supernatant, and removes the yeast from the obtained useful component eluate. Therefore, according to the method of the second embodiment, it is possible to produce a tobacco flavor liquid that contains a large amount of useful components and has a reduced microbial load.
＜2.烟草香味液＞＜2.Tobacco flavor liquid＞
根据另一方面，本发明提供一种烟草香味液，其是通过上述的“烟草香味液的制造方法”而制造的烟草香味液。According to another aspect, the present invention provides a tobacco flavor liquid produced by the above-mentioned "tobacco flavor liquid manufacturing method".
如上所述，烟草香味液可以为以下的任意烟草香味液：As mentioned above, the tobacco flavor liquid can be any of the following tobacco flavor liquids:
(i)通过第1实施方式的方法得到的“无微生物的烟草上清”；以及(i) "Microorganism-free tobacco supernatant" obtained by the method of the first embodiment; and
(ii)通过第2实施方式的方法得到的“无微生物的含有用成分液体”。(ii) "Microorganism-free component-containing liquid" obtained by the method of the second embodiment.
因此，通过上述的“烟草香味液的制造方法”制造的烟草香味液包含上述2种产物作为具体例。Therefore, the tobacco flavor liquid manufactured by the above-mentioned "Tobacco flavor liquid manufacturing method" includes the above two products as specific examples.
如上所述，烟草香味液已去除了微生物，因此，能够消除烟草香味液的组成因微生物的代谢而发生变化的可能性，作为香味抽吸器的香味源使用时，从品质稳定性的观点考虑是优异的。As mentioned above, microorganisms have been removed from the tobacco flavor liquid. Therefore, the possibility of the composition of the tobacco flavor liquid changing due to the metabolism of microorganisms can be eliminated. When used as a flavor source for a flavor inhaler, it is considered from the perspective of quality stability. is excellent.
尤其是通过第2实施方式的方法得到的烟草香味液是通过上述的第2实施方式的方法制造的，因此，能够包含大量的有用成分。因此，在将上述烟草香味液导入香味抽吸器的情况下，能够在香味抽吸器中显著地发挥有用成分的效果。例如，在有用成分为香味贡献成分的情况下，烟草香味液可以包含大量的香味贡献成分，因此，在导入香味抽吸器等烟草制品的情况下，能够向使用者提供增强的香味。In particular, the tobacco flavor liquid obtained by the method of the second embodiment is produced by the method of the second embodiment described above, and therefore can contain a large amount of useful components. Therefore, when the tobacco flavor liquid is introduced into the flavor inhaler, the effect of the useful component can be significantly exerted in the flavor inhaler. For example, when the useful component is a flavor-contributing component, the tobacco flavor liquid may contain a large amount of the flavor-contributing component. Therefore, when introduced into tobacco products such as a flavor inhaler, an enhanced flavor can be provided to the user.
可以通过公知技术将通过上述的“烟草香味液的制造方法”制造的烟草香味液导入香味抽吸器等烟草制品。以下，对烟草香味液的使用例进行说明。The tobacco flavor liquid produced by the above-mentioned "Method for producing tobacco flavor liquid" can be introduced into tobacco products such as flavor inhalers using known techniques. Hereinafter, examples of use of tobacco flavor liquid will be described.
例如，可以将烟草香味液添加于烟草材料(例如，去筋烟叶、烟叶)、并使得到的混合物干燥而使用。For example, the tobacco flavor liquid can be added to tobacco materials (for example, filler tobacco leaves, tobacco leaves), and the resulting mixture can be dried and used.
或者，可以将烟草香味液添加于上述的提取工序(S1)中得到的烟草残渣，由得到的混合物制作片状烟草、烟草颗粒等烟草成形体，将烟草成形体作为烟草制品的烟草香味源来使用。Alternatively, the tobacco flavor liquid can be added to the tobacco residue obtained in the above-mentioned extraction step (S1), and tobacco molded objects such as sheet tobacco and tobacco particles can be produced from the obtained mixture, and the tobacco molded objects can be used as the tobacco flavor source of tobacco products. use.
或者，可以将烟草香味液添加于上述的提取工序(S1)中得到的烟草残渣，将得到的混合物干燥并粉碎，制作烟草粉末，将烟草粉末添加于烟草材料(例如，去筋烟叶、烟叶)来使用。Alternatively, the tobacco flavor liquid can be added to the tobacco residue obtained in the above-mentioned extraction step (S1), the obtained mixture can be dried and pulverized to prepare tobacco powder, and the tobacco powder can be added to the tobacco material (for example, cut tobacco leaves, tobacco leaves) to use.
或者，可以将烟草香味液添加于上述的提取工序(S1)中得到的烟草残渣，将得到的混合物干燥并粉碎，制作烟草粉末，使烟草粉末悬浮在水中，制备烟草浆料，将烟草浆料添加于烟草材料(例如，去筋烟叶、烟叶)来使用。Alternatively, the tobacco flavor liquid can be added to the tobacco residue obtained in the above-mentioned extraction step (S1), the obtained mixture can be dried and pulverized to prepare tobacco powder, the tobacco powder can be suspended in water to prepare a tobacco slurry, and the tobacco slurry can be It is added to tobacco materials (for example, filler tobacco leaves, tobacco leaves) and used.
或者，可以通过公知技术将烟草香味液胶囊化，并将得到的香料胶囊导入烟草制品的滤嘴部分。Alternatively, the tobacco flavor liquid can be encapsulated by known techniques, and the obtained flavor capsules can be introduced into the filter part of the tobacco product.
＜3.烟草添加物＞＜3.Tobacco additives＞
如上所述，烟草香味液可以与上述的提取工序(S1)中得到的烟草残渣组合使用。因此，根据另一方面，可以提供一种烟草添加物，其包含：As mentioned above, the tobacco flavor liquid can be used in combination with the tobacco residue obtained in the above-mentioned extraction step (S1). Accordingly, according to another aspect, a tobacco additive may be provided, comprising:
通过上述的“烟草香味液的制造方法”制造的烟草香味液、和Tobacco flavor liquid manufactured by the above-mentioned "Tobacco flavor liquid manufacturing method", and
在上述的“烟草香味液的制造方法”中得到烟草上清时所得到的烟草残渣。The tobacco residue obtained when the tobacco supernatant is obtained in the above-mentioned "Method for Manufacturing Tobacco Flavor Liquid".
以下，对烟草添加物的具体例进行说明。Specific examples of tobacco additives will be described below.
例如，烟草添加物可以是通过使烟草香味液与提取工序(S1)中得到的烟草残渣的混合物进行干燥而得到的产物。该产物可以用作烟草制品的烟草香味源。For example, the tobacco additive may be a product obtained by drying a mixture of tobacco flavor liquid and tobacco residue obtained in the extraction step (S1). The product can be used as a tobacco flavor source for tobacco products.
或者，烟草添加物可以是通过将烟草香味液与提取工序(S1)中得到的烟草残渣的混合物成形为片形状、颗粒形状等特定形状而得到的烟草成形体。烟草成形体可以用作烟草制品的烟草香味源。Alternatively, the tobacco additive may be a tobacco molded body obtained by molding a mixture of the tobacco flavor liquid and the tobacco residue obtained in the extraction step (S1) into a specific shape such as a sheet shape or a granular shape. The tobacco shaped body can be used as a tobacco flavor source for tobacco products.
或者，烟草添加物可以是通过将烟草香味液与提取工序(S1)中得到的烟草残渣的混合物干燥并粉碎成粉末状而得到的烟草粉末。烟草粉末可以通过添加于烟草材料(例如，去筋烟叶、烟叶)中来增强烟草材料的香味。Alternatively, the tobacco additive may be tobacco powder obtained by drying and pulverizing a mixture of tobacco flavor liquid and tobacco residue obtained in the extraction step (S1) into powder form. Tobacco powder can be added to tobacco materials (eg, filler tobacco leaves, tobacco leaves) to enhance the flavor of tobacco materials.
或者，烟草添加物可以是通过将烟草香味液与提取工序(S1)中得到的烟草残渣的混合物干燥、粉碎成粉末状、并使得到的粉末悬浮于水中而得到的烟草浆料。烟草浆料可以通过添加于烟草材料(例如，去筋烟叶、烟叶)中来增强烟草材料的香味。Alternatively, the tobacco additive may be tobacco slurry obtained by drying and pulverizing a mixture of tobacco flavor liquid and tobacco residue obtained in the extraction step (S1) into powder, and suspending the obtained powder in water. Tobacco slurry can be added to tobacco materials (eg, filler tobacco leaves, tobacco leaves) to enhance the flavor of tobacco materials.
根据需要，烟草添加物可以包含粘合剂、pH调整剂、防腐剂、抗氧剂等添加剂。As needed, tobacco additives may include additives such as binders, pH adjusters, preservatives, and antioxidants.
＜4.香味抽吸器＞＜4.Fragrance inhaler＞
可以将上述的“烟草香味液”或上述的“烟草添加物”导入任意的烟草制品。作为典型例，可以将上述的“烟草香味液”或上述的“烟草添加物”导入燃烧型香味抽吸器、加热型香味抽吸器等香味抽吸器。即，根据另一方面，可以提供包含上述的“烟草香味液”的香味抽吸器或包含上述的“烟草添加物”的香味抽吸器。The above-mentioned "tobacco flavor liquid" or the above-mentioned "tobacco additive" can be introduced into any tobacco product. As a typical example, the above-mentioned "tobacco flavor liquid" or the above-mentioned "tobacco additive" can be introduced into a flavor inhaler such as a combustion type flavor inhaler or a heating type flavor inhaler. That is, according to another aspect, it is possible to provide a flavor inhaler containing the above-mentioned "tobacco flavor liquid" or a flavor inhaler containing the above-mentioned "tobacco additive".
上述的“烟草香味液”或上述的“烟草添加物”只要能够在使使用者在使用烟草制品时品味到增强的香味即可，可以导入烟草制品的任意位置。The above-mentioned "tobacco flavor liquid" or the above-mentioned "tobacco additive" can be introduced into any position of the tobacco product as long as it allows the user to taste an enhanced flavor when using the tobacco product.
作为燃烧型香味抽吸器的例子，可举出香烟、烟斗、烟袋、雪茄、或小雪茄(CIGARILLO)等。将燃烧型香味抽吸器的一例、即典型构成的香烟示于图3。Examples of combustion-type flavor inhalers include cigarettes, pipes, pouches, cigars, or cigarillos. An example of a combustion type flavor inhaler, that is, a cigarette with a typical structure is shown in FIG. 3 .
图3所示的燃烧型香味抽吸器1包含：The combustion type fragrance inhaler 1 shown in Figure 3 contains:
包含烟草填充材料2a和对烟草填充材料2a的周围进行卷装的烟草卷纸2b的烟杆2、Cigarette rod 2 including a tobacco filling material 2a and a tobacco rolling paper 2b wrapped around the tobacco filling material 2a,
包含滤材3a和卷绕于滤材3a的周围的滤棒成形纸3b的滤嘴3、以及以将烟杆2与滤嘴3连接的方式卷绕于烟杆2和滤嘴3上的接装纸4。The filter tip 3 includes a filter material 3a and a filter rod forming paper 3b wound around the filter material 3a, and a connector wound around the cigarette rod 2 and the filter tip 3 to connect the cigarette rod 2 and the filter tip 3. Load paper 4.
烟杆2包含烟丝、烟草成形体等烟草填充材料2a。烟杆与通常的香烟相同，例如可以具有5～10mm的直径及40～80mm的长度。The cigarette rod 2 contains tobacco filler material 2a such as shredded tobacco and tobacco molding. The cigarette rod is the same as a normal cigarette, and may have a diameter of 5 to 10 mm and a length of 40 to 80 mm, for example.
滤嘴3是由单一的滤材3a形成的滤嘴、所谓单滤嘴。滤材3a与通常的香烟相同，可以由纤维素乙酸酯丝束等滤材构成。滤嘴3具有与烟杆2基本相同的直径，长度与通常的香烟相同，例如可以为15～40mm。滤棒成形纸3b可以为10～100μm的厚度，其通气性的有无没有限定，但一般使用具有通气性的纸。The filter 3 is a filter formed of a single filter material 3a, a so-called single filter. The filter material 3a can be made of a filter material such as cellulose acetate tow, similar to a normal cigarette. The filter tip 3 has substantially the same diameter as the cigarette rod 2, and the length is the same as that of a normal cigarette, for example, it can be 15 to 40 mm. The filter rod forming paper 3b may have a thickness of 10 to 100 μm, and the presence or absence of air permeability is not limited, but generally, paper having air permeability is used.
可以以覆盖滤棒成形纸3b整体和烟草卷纸2b的一部分的方式用粘接剂对接装纸4进行粘接。接装纸4的例如烟杆的轴向的长度(宽度)可以为20～50mm，厚度可以为10～100μm。与通常的香烟相同，可以在接装纸4上沿着香烟的圆周方向穿设一列、多列、或不规则的多个通气用的小开孔(通风孔)。The tipping paper 4 can be bonded with an adhesive so as to cover the entire plug forming paper 3b and a part of the tobacco rolling paper 2b. For example, the axial length (width) of the cigarette rod of the tipping paper 4 can be 20 to 50 mm, and the thickness can be 10 to 100 μm. Similar to ordinary cigarettes, the tipping paper 4 can be provided with one row, multiple rows, or a plurality of irregular small openings (vents) for ventilation along the circumferential direction of the cigarette.
在图3所示的燃烧型香味抽吸器1的情况下，可以将上述的“烟草香味液”、上述的“烟草添加物”导入例如烟草填充材料2a或滤材3a。In the case of the combustion type flavor inhaler 1 shown in FIG. 3, the above-mentioned "tobacco flavor liquid" and the above-mentioned "tobacco additive" can be introduced into, for example, the tobacco filling material 2a or the filter material 3a.
接下来，对加热型香味抽吸器的例子进行说明。作为加热型香味抽吸器的例子，可举出：Next, an example of a heated fragrance inhaler will be described. Examples of heated aroma inhalers include:
利用碳热源的燃烧热对烟草填充材料进行加热的碳热源型抽吸器(例如参照WO2006/073065)；A carbon heat source type smoker that uses the combustion heat of a carbon heat source to heat tobacco filling materials (for example, refer to WO2006/073065);
具备包含烟草填充材料的烟草棒和用于对烟草棒进行电加热的加热设备的电加热型抽吸器(例如参照WO2010/110226)；或者An electrically heated smoker having a tobacco rod containing tobacco filler material and a heating device for electrically heating the tobacco rod (see, for example, WO2010/110226); or
通过加热器对液态的气溶胶源进行加热，产生气溶胶，将来自烟草填充材料的香味与气溶胶一起抽吸的液体雾化型抽吸器(例如参照WO2015/046385)等。A liquid atomization type inhaler is used to heat a liquid aerosol source with a heater to generate aerosol, and inhales the aroma from the tobacco filler material together with the aerosol (see, for example, WO2015/046385).
以下，参照图4～6对加热型香味抽吸器的一例进行说明。图4是示出加热型香味抽吸器的一例的立体图。图5是示出烟草棒的内部结构的图。图6是示出气溶胶生成装置的内部结构的图。Hereinafter, an example of the heating type fragrance inhaler will be described with reference to FIGS. 4 to 6 . FIG. 4 is a perspective view showing an example of the heating type fragrance inhaler. Fig. 5 is a diagram showing the internal structure of the tobacco rod. FIG. 6 is a diagram showing the internal structure of the aerosol generating device.
如图4所示，加热型香味抽吸器100具有：As shown in Figure 4, the heated fragrance inhaler 100 has:
包含烟草填充材料和气溶胶源的烟草棒110、和Tobacco rod 110 containing tobacco filler material and aerosol source, and
以可装卸的方式安装烟草棒110的气溶胶生成装置120，该气溶胶生成装置120对烟草棒110进行加热，由气溶胶源产生气溶胶，并且通过气溶胶的作用从烟草填充材料中释放香味成分。The aerosol generating device 120 of the tobacco rod 110 is detachably installed. The aerosol generating device 120 heats the tobacco rod 110, generates aerosol from the aerosol source, and releases flavor from the tobacco filler material through the action of the aerosol. Element.
烟草棒110是可交换的筒(carrtridge)，具有沿着长度方向延伸的柱状形状。烟草棒110以通过在插入气溶胶生成装置120的状态下进行加热从而产生气溶胶及香味成分的方式构成。The tobacco rod 110 is an exchangeable cartridge having a cylindrical shape extending along its length. The tobacco rod 110 is heated while being inserted into the aerosol generating device 120 to generate aerosol and flavor components.
如图5所示，烟草棒110具有：包含填充物111和对填充物111进行卷装的第1卷纸112的基材部11A、以及形成与基材部11A相反侧的端部的吸口部11B。基材部11A与吸口部11B通过第2卷纸113而连结在一起。As shown in FIG. 5 , the tobacco rod 110 has a base portion 11A including a filler 111 and a first paper roll 112 for wrapping the filler 111 , and a mouth portion forming an end opposite to the base portion 11A. 11B. The base material part 11A and the suction mouth part 11B are connected together by the 2nd roll paper 113.
吸口部11B具有：纸管部114、滤嘴部115、以及配置于纸管部114与滤嘴部115之间的中空区段部116。纸管部114是将纸卷成圆筒形而形成的纸管，内侧为空腔。滤嘴部115包含纤维素乙酸酯丝束等滤材。中空区段部116包含具有1个或多个中空通道的填充层。滤嘴部115的滤材与中空区段部116的填充层通过用滤棒成形纸117覆盖而连结在一起。填充层由纤维构成，纤维的填充密度高，因此，在抽吸时，空气、气溶胶仅流过中空通道，基本上不流过填充层内。在烟草棒110中，在想要减小由在滤嘴部115的气溶胶成分的过滤所导致的减少时，缩短滤嘴部115的长度并用中空区段部116进行置换对于增大气溶胶的传送量而言是有效的。The suction mouth part 11B has the paper tube part 114, the filter part 115, and the hollow segment part 116 arrange|positioned between the paper tube part 114 and the filter part 115. The paper tube part 114 is a paper tube formed by rolling paper into a cylindrical shape, and has a cavity inside. The filter part 115 contains a filter material such as cellulose acetate tow. The hollow section 116 includes a filling layer having one or more hollow channels. The filter material of the filter tip portion 115 and the filling layer of the hollow segment portion 116 are connected together by covering them with filter rod forming paper 117 . The filling layer is composed of fibers, and the filling density of the fibers is high. Therefore, during suction, air and aerosol only flow through the hollow channels and basically do not flow through the filling layer. In the tobacco rod 110, when it is desired to reduce the reduction caused by the filtration of aerosol components in the filter portion 115, shortening the length of the filter portion 115 and replacing it with the hollow segment portion 116 can increase the transmission of aerosols. Quantitatively effective.
吸口部11B由3个区段构成，但吸口部11B也可以由1个或2个区段构成，还可以由4个或4个以上区段构成。例如，也可以省略中空区段部116，将纸管部114与滤嘴部115彼此相邻地配置而形成吸口部11B。The mouthpiece 11B is composed of three segments, but the mouthpiece 11B may be composed of one or two segments, or may be composed of four or more segments. For example, the hollow segment 116 may be omitted, and the paper tube part 114 and the filter part 115 may be arranged adjacent to each other to form the suction mouth part 11B.
烟草棒110的长度方向的长度优选为40～90mm、更优选为50～75mm、进一步优选为50～60mm。烟草棒110的圆周优选为15～25mm、更优选为17～24mm、进一步优选为20～23mm。另外，在烟草棒110的长度方向上，基材部11A的长度可以为20mm，纸管部114的长度可以为20mm，中空区段部116的长度可以为8mm，滤嘴部115的长度可以为7mm，上述各个区段的长度可以根据制造适应性、要求品质等而适当变更。The length of the tobacco rod 110 in the longitudinal direction is preferably 40 to 90 mm, more preferably 50 to 75 mm, and even more preferably 50 to 60 mm. The circumference of the tobacco rod 110 is preferably 15 to 25 mm, more preferably 17 to 24 mm, and even more preferably 20 to 23 mm. In addition, in the length direction of the tobacco rod 110, the length of the base material part 11A may be 20 mm, the length of the paper tube part 114 may be 20 mm, the length of the hollow section part 116 may be 8 mm, and the length of the filter part 115 may be 7mm, the length of each of the above sections can be appropriately changed according to manufacturing adaptability, required quality, etc.
填充物111包含烟草填充材料和气溶胶源。气溶胶源以给定温度被加热而产生气溶胶。作为气溶胶源，例如可举出：甘油、丙二醇、三乙酸甘油酯、1,3-丁二醇、以及它们的混合物。填充物111中的气溶胶源的含量没有特别限定，从产生足够量的气溶胶和赋予良好的香吸味的观点考虑，通常为5质量％以上，优选为10质量％以上，另外，通常为50质量％以下，优选为20质量％以下。Filler 111 contains tobacco filler material and an aerosol source. The aerosol source is heated at a given temperature to produce aerosols. Examples of the aerosol source include glycerin, propylene glycol, triacetin, 1,3-butanediol, and mixtures thereof. The content of the aerosol source in the filler 111 is not particularly limited. From the viewpoint of generating a sufficient amount of aerosol and imparting a good aroma, it is usually 5 mass % or more, preferably 10 mass % or more. In addition, it is usually 5 mass % or more. 50 mass% or less, preferably 20 mass% or less.
如以上所说明的那样，烟草填充材料例如具有烟丝的形态或烟草成形体的形态。在烟草填充材料具有烟丝的形态的情况下，可以具有通过将烟叶(即完成熟化的烟草叶)裁切成例如0.8～1.2mm的宽度而得到的烟丝的形态。或者，在烟草填充材料具有片状烟草的形态的情况下，可以具有通过将片状烟草裁切成例如0.8～1.2mm的宽度而得到的细长的片状烟草的形态，或者，可以具有通过对片状烟草不进行切丝而进行褶裥加工所得到的波浪形的片状烟草的形态。As described above, the tobacco filler material has, for example, the form of cut tobacco or the form of a tobacco molded body. When the tobacco filler material has the form of shredded tobacco, it may have the form of shredded tobacco obtained by cutting tobacco leaves (that is, matured tobacco leaves) into a width of, for example, 0.8 to 1.2 mm. Alternatively, when the tobacco filling material has the form of sheet tobacco, it may have the form of an elongated sheet tobacco obtained by cutting the sheet tobacco into a width of, for example, 0.8 to 1.2 mm, or may have the form of a sheet of tobacco. The shape of a corrugated sheet tobacco obtained by pleating the sheet tobacco without cutting it into shreds.
在基材部11A为圆周22mm、长度20mm的情况下，烟草棒110中的填充物111的含量例如为200～400mg，优选为250～320mg。填充物111的水分含量例如为8～18质量％，优选为10～16质量％。为这样的水分含量时，可抑制卷染的发生，使基材部11A在制造时的卷起适应性良好。When the base material portion 11A has a circumference of 22 mm and a length of 20 mm, the content of the filler 111 in the tobacco rod 110 is, for example, 200 to 400 mg, preferably 250 to 320 mg. The moisture content of the filler 111 is, for example, 8 to 18 mass%, preferably 10 to 16 mass%. When the moisture content is such, the occurrence of jig dyeing can be suppressed, and the rolling adaptability of the base material portion 11A during production can be improved.
第1卷纸112、第2卷纸113及滤棒成形纸117分别可以使用与在香烟中使用的烟草卷纸、接装纸及滤棒成形纸相同的材料。The first rolling paper 112, the second rolling paper 113, and the filter plug forming paper 117 can respectively use the same materials as the tobacco rolling paper, tipping paper, and filter plug forming paper used in cigarettes.
在图5所示的烟草棒110的情况下，可以将上述的“烟草香味液”、上述的“烟草添加物”导入例如填充物111或滤嘴部115的滤材。In the case of the tobacco rod 110 shown in FIG. 5 , the above-mentioned “tobacco flavor liquid” and the above-mentioned “tobacco additive” may be introduced into the filter material of the filler 111 or the filter part 115 , for example.
如图6所示，气溶胶生成装置120具有能够插入烟草棒110的插入孔130。即，气溶胶生成装置120具有构成插入孔130的内侧筒构件132。内侧筒构件132例如可以由如铝、不锈钢(SUS)这样的导热构件构成。As shown in FIG. 6 , the aerosol generating device 120 has an insertion hole 130 into which the tobacco rod 110 can be inserted. That is, the aerosol generating device 120 has the inner cylindrical member 132 constituting the insertion hole 130 . The inner cylinder member 132 may be made of a thermally conductive member such as aluminum or stainless steel (SUS).
另外，气溶胶生成装置120可以具有将插入孔130闭塞的盖部140。盖部140以能够在将插入孔130闭塞的状态与使插入孔130露出的状态(参照图4)之间滑动的方式构成。In addition, the aerosol generating device 120 may have a cover 140 that blocks the insertion hole 130 . The cover 140 is configured to be slidable between a state in which the insertion hole 130 is closed and a state in which the insertion hole 130 is exposed (see FIG. 4 ).
气溶胶生成装置120可以具有与插入孔130连通的空气流路160。空气流路160的一端与插入孔130连结在一起，空气流路160的另一端在与插入孔130不同的位置与气溶胶生成装置120的外部(外部大气)连通。The aerosol generating device 120 may have an air flow path 160 communicating with the insertion hole 130 . One end of the air flow path 160 is connected to the insertion hole 130 , and the other end of the air flow path 160 is connected to the outside of the aerosol generation device 120 (outside atmosphere) at a different position from the insertion hole 130 .
气溶胶生成装置120可以具有覆盖空气流路160的与外部气体连通的一侧的端部的盖部170。盖部170可以处于覆盖空气流路160的与外部气体连通的一侧的端部的状态，或者也可以处于使空气流路160露出的状态。The aerosol generation device 120 may include a cover 170 that covers an end portion of the air flow path 160 that communicates with external air. The cover 170 may be in a state of covering the end of the air flow path 160 that communicates with the outside air, or may be in a state of exposing the air flow path 160 .
盖部170即使在覆盖空气流路160的状态下，也不会将空气流路160气密性地闭塞。即，盖部170以即使在覆盖空气流路160的状态下、外部大气也可以经由盖部170附近而流入空气流路160内的方式构成。Even in a state of covering the air flow path 160 , the cover portion 170 does not airtightly block the air flow path 160 . That is, even when the cover 170 covers the air flow path 160 , external air can flow into the air flow path 160 through the vicinity of the cover 170 .
使用者在将烟草棒110插入了气溶胶生成装置120的状态下，叼住烟草棒110的一端部、具体为图5所示的吸口部11B，进行抽吸动作。通过使用者的抽吸动作，外部气体流入空气流路160。流入空气流路160内的空气从插入孔130内的烟草棒110通过，被导入使用者的口腔内。With the tobacco rod 110 inserted into the aerosol generating device 120, the user holds one end of the tobacco rod 110, specifically the mouthpiece 11B shown in FIG. 5, and performs a smoking action. The outside air flows into the air flow path 160 through the user's suction action. The air flowing into the air flow path 160 passes through the tobacco rod 110 in the insertion hole 130 and is introduced into the user's oral cavity.
气溶胶生成装置120可以在空气流路160内或构成空气流路160的壁部的外表面具有温度传感器。温度传感器例如可以为热敏电阻、热电偶等。在使用者对烟草棒110的吸口部11B进行抽吸时，由于从盖部170一侧朝向加热器30一侧流过空气流路160内的空气的影响，空气流路160的内部温度或构成空气流路160的壁部的温度会降低。温度传感器可以通过对该温度降低进行测定而检测使用者的抽吸动作。The aerosol generating device 120 may have a temperature sensor inside the air flow path 160 or on an outer surface of a wall constituting the air flow path 160 . The temperature sensor may be, for example, a thermistor, a thermocouple, or the like. When the user smokes the mouthpiece portion 11B of the tobacco rod 110, due to the influence of the air flowing through the air flow path 160 from the cover portion 170 side toward the heater 30 side, the internal temperature or composition of the air flow path 160 changes. The temperature of the wall portion of the air flow path 160 decreases. The temperature sensor can detect the user's puffing action by measuring this temperature drop.
气溶胶生成装置120具有电池10、控制单元20、以及加热器30。电池10储存气溶胶生成装置120所使用的电力。电池10可以为能够充放电的二次电池。电池10例如可以为锂离子电池。The aerosol generating device 120 has a battery 10 , a control unit 20 , and a heater 30 . The battery 10 stores power used by the aerosol generating device 120 . The battery 10 may be a chargeable and dischargeable secondary battery. The battery 10 may be, for example, a lithium ion battery.
加热器30可以设置于内侧筒构件132的周围。容纳加热器30的空间和容纳电池10的空间可以通过间隔壁180而相互分离。由此，能够抑制被加热器30加热后的空气流入容纳电池10的空间内。因此，能够抑制电池10的温度上升。The heater 30 may be provided around the inner cylinder member 132 . The space accommodating the heater 30 and the space accommodating the battery 10 may be separated from each other by the partition wall 180 . This can prevent the air heated by the heater 30 from flowing into the space accommodating the battery 10 . Therefore, the temperature rise of the battery 10 can be suppressed.
加热器30优选为能够对柱状的烟草棒110的外周进行加热的筒形状。加热器30例如可以为膜加热器。膜加热器可以具有一对膜状的基板和夹在一对基板之间的电阻发热体。膜状的基板优选由耐热性及电绝缘性优异的材料制作，典型地由聚酰亚胺制作。电阻发热体优选由铜、镍合金、铬合金、不锈钢、铂铑等金属材料中的1种或2种以上制作，例如，可以由不锈钢制的基材而形成。此外，为了通过柔性印刷电路(FPC)将电阻发热体与电源连接，可以对连接部位及其引线部实施镀铜。The heater 30 preferably has a cylindrical shape capable of heating the outer periphery of the columnar tobacco rod 110 . The heater 30 may be a film heater, for example. The film heater may have a pair of film-shaped substrates and a resistance heating element sandwiched between the pair of substrates. The film-like substrate is preferably made of a material excellent in heat resistance and electrical insulation, and is typically made of polyimide. The resistance heating element is preferably made of one or more metal materials such as copper, nickel alloy, chromium alloy, stainless steel, platinum and rhodium. For example, it can be formed of a base material made of stainless steel. In addition, in order to connect the resistance heating element to the power supply through a flexible printed circuit (FPC), copper plating can be performed on the connection part and its lead part.
优选可以在加热器30的外侧设置有热收缩管。热收缩管是通过热在半径方向上收缩的管，例如由热塑性弹性体构成。通过热收缩管的收缩作用将加热器30按压于内侧筒构件132。由此，加热器30与内侧筒构件132的密合性增高，因此，从加热器30经由内侧筒构件132向烟草棒110的导热性增高。Preferably, a heat shrinkable tube may be provided outside the heater 30 . The heat-shrinkable tube is a tube that shrinks in the radial direction by heat, and is made of, for example, a thermoplastic elastomer. The heater 30 is pressed against the inner cylindrical member 132 by the contraction of the heat shrink tube. Thereby, the adhesion between the heater 30 and the inner cylindrical member 132 increases, and therefore the thermal conductivity from the heater 30 to the tobacco rod 110 via the inner cylindrical member 132 increases.
气溶胶生成装置120可以在加热器30的半径方向的外侧、优选为热收缩管的外侧具有筒状的绝热材料。通过隔绝加热器30的热，绝热材料能够发挥防止气溶胶生成装置120的框体外表面达到过高的高温的作用。绝热材料例如可以由二氧化硅气凝胶、碳气凝胶、氧化铝气凝胶等气凝胶而制作。典型地，作为绝热材料的气凝胶可以为绝热性能高且制造成本较低的二氧化硅气凝胶。然而，绝热材料也可以是玻璃棉、石棉等纤维类绝热材料，还可以为聚氨酯泡沫、酚醛泡沫的发泡类绝热材料。或者，绝热材料可以为真空绝热材料。The aerosol generating device 120 may have a cylindrical heat insulating material outside the heater 30 in the radial direction, preferably outside the heat shrink tube. By blocking the heat of the heater 30, the heat insulating material can play a role in preventing the outer surface of the housing of the aerosol generating device 120 from reaching an excessively high temperature. The thermal insulating material can be made of aerogels such as silica aerogel, carbon aerogel, and alumina aerogel. Typically, the aerogel used as a thermal insulation material can be silica aerogel with high thermal insulation performance and low manufacturing cost. However, the thermal insulation material can also be fiber insulation materials such as glass wool and asbestos, or foam insulation materials such as polyurethane foam and phenolic foam. Alternatively, the insulation material may be a vacuum insulation material.
绝热材料可以设置于面对烟草棒110的内侧筒构件132与绝热材料的外侧的外侧筒构件134之间。外侧筒构件134可以由例如铝、不锈钢(SUS)这样的导热构件构成。绝热材料优选设置于密闭的空间内。Insulating material may be provided between the inner barrel member 132 facing the tobacco rod 110 and the outer barrel member 134 on the outside of the insulating material. The outer cylinder member 134 may be made of a thermally conductive member such as aluminum or stainless steel (SUS). The thermal insulation material is preferably provided in a closed space.
控制单元20可以包含控制基板、CPU及存储器等。另外，气溶胶生成装置120可以具有用于在基于控制单元20的控制下向使用者报告各种信息的通知部。通知部可以为例如LED这样的发光元件或振动元件、或者它们的组合。The control unit 20 may include a control substrate, a CPU, a memory, and the like. In addition, the aerosol generating device 120 may have a notification unit for reporting various information to the user under control of the control unit 20 . The notification unit may be a light-emitting element such as an LED, a vibrating element, or a combination thereof.
控制单元20检测到使用者的启动请求后，开始从电池10向加热器30的电力供给。使用者的启动请求例如可以通过使用者的按钮、滑动式开关的操作、使用者的抽吸动作而实行。使用者的启动请求可以通过按下按钮150而实行。更具体而言，使用者的启动请求可以通过在打开了盖部140的状态下按下按钮150而实行。或者，使用者的启动请求可以通过检测使用者的抽吸动作而实行。使用者的抽吸动作可以通过例如如上所述的温度传感器而检测。After detecting the user's activation request, the control unit 20 starts supplying electric power from the battery 10 to the heater 30 . The user's activation request can be implemented, for example, by the user's operation of a button, a slide switch, or the user's suction action. The user's activation request can be implemented by pressing button 150 . More specifically, the user's activation request can be executed by pressing the button 150 with the cover 140 opened. Alternatively, the user's activation request may be implemented by detecting the user's puffing action. The user's puffing action can be detected by, for example, a temperature sensor as described above.
＜5.优选的实施方式＞<5. Preferred embodiment>
以下，总结示出优选的实施方式。Hereinafter, preferred embodiments are summarized and shown.
[A1]一种烟草香味液的制造方法，该方法包括：[A1] A method for manufacturing tobacco flavor liquid, which method includes:
用包含无机多孔体的材料对烟草提取液进行处理，将微生物从上述烟草提取液中去除。The tobacco extract liquid is treated with a material containing an inorganic porous body to remove microorganisms from the tobacco extract liquid.
[A2]一种烟草香味液的制造方法，该方法包括：[A2] A method for manufacturing tobacco flavor liquid, the method includes:
用水性溶剂从烟草材料中提取上述烟草材料所包含的水溶性成分，得到烟草上清；和Extract the water-soluble components contained in the tobacco material from the tobacco material with a water-based solvent to obtain the tobacco supernatant; and
用包含无机多孔体的材料对上述烟草上清进行处理，得到无微生物的烟草上清。The above-mentioned tobacco supernatant is treated with a material containing an inorganic porous body to obtain a microorganism-free tobacco supernatant.
[A3]一种烟草香味液的制造方法，该方法包括：[A3] A method for manufacturing tobacco flavor liquid, the method includes:
用水性溶剂从烟草材料中提取上述烟草材料所包含的水溶性成分，得到烟草上清；Extract the water-soluble components contained in the tobacco material from the tobacco material with a water-based solvent to obtain the tobacco supernatant;
在上述烟草上清中培养酵母，得到含酵母培养液；Cultivate yeast in the above-mentioned tobacco supernatant to obtain a yeast-containing culture solution;
将上述含酵母培养液与包含有机溶剂的溶出溶剂混合，使上述酵母的菌体内包含的有用成分从得到的混合物中含有的上述酵母溶出至上述混合物的液体部分，得到有用成分溶出液；以及The above-mentioned yeast-containing culture liquid is mixed with an elution solvent containing an organic solvent, and the useful components contained in the cells of the above-mentioned yeast are eluted from the above-mentioned yeast contained in the obtained mixture to the liquid part of the above-mentioned mixture, thereby obtaining a useful component eluate; and
用包含无机多孔体的材料对上述有用成分溶出液进行处理，得到无微生物的有用成分溶出液。The above-mentioned useful component eluate is treated with a material containing an inorganic porous body to obtain a microorganism-free useful component eluate.
[A4]根据[A1]所述的方法，其中，[A4] The method according to [A1], wherein,
上述处理通过使上述烟草提取液通过包含无机多孔体的层而进行。The above treatment is performed by passing the tobacco extract liquid through a layer containing an inorganic porous body.
[A5]根据[A2]所述的方法，其中，[A5] The method according to [A2], wherein,
上述处理通过使上述烟草上清通过包含无机多孔体的层而进行。The above treatment is performed by passing the tobacco supernatant through a layer containing an inorganic porous body.
[A6]根据[A3]所述的方法，其中，[A6] The method according to [A3], wherein,
上述处理通过使上述有用成分溶出液通过包含无机多孔体的层而进行。The above-mentioned treatment is performed by passing the above-mentioned useful component eluate through a layer containing an inorganic porous body.
[A7]根据[A1]所述的方法，其中，[A7] The method according to [A1], wherein,
上述处理通过使上述烟草提取液通过包含无机多孔体的成形体而进行。The above treatment is performed by passing the tobacco extract liquid through a molded body containing an inorganic porous body.
[A8]根据[A2]所述的方法，其中，[A8] The method according to [A2], wherein,
上述处理通过使上述烟草上清通过包含无机多孔体的成形体而进行。The above treatment is performed by passing the tobacco supernatant through a molded body containing an inorganic porous body.
[A9]根据[A3]所述的方法，其中，[A9] The method according to [A3], wherein,
上述处理通过使上述有用成分溶出液通过包含无机多孔体的成形体而进行。The above-mentioned treatment is performed by passing the above-mentioned useful component eluate through a molded body containing an inorganic porous body.
[A10]根据[A1]所述的方法，其中，[A10] The method according to [A1], wherein,
上述处理通过使上述烟草提取液通过包含无机多孔体的粒子的集合体而进行。The above treatment is performed by passing the tobacco extract liquid through an aggregate of particles including inorganic porous bodies.
[A11]根据[A2]所述的方法，其中，[A11] The method according to [A2], wherein,
上述处理通过使上述烟草上清通过包含无机多孔体的粒子的集合体而进行。The above treatment is performed by passing the tobacco supernatant through an assembly of particles containing an inorganic porous body.
[A12]根据[A3]所述的方法，其中，[A12] The method according to [A3], wherein,
上述处理通过使上述有用成分溶出液通过包含无机多孔体的粒子的集合体而进行。The above-mentioned treatment is performed by passing the above-mentioned useful component eluate through an aggregate of particles containing an inorganic porous body.
[A13]根据[A1]所述的方法，其中，[A13] The method according to [A1], wherein,
上述处理通过使上述烟草提取液通过填充有包含无机多孔体的粒子的柱而进行。The above treatment is performed by passing the tobacco extract liquid through a column filled with particles containing inorganic porous bodies.
[A14]根据[A2]所述的方法，其中，[A14] The method according to [A2], wherein,
上述处理通过使上述烟草上清通过填充有包含无机多孔体的粒子的柱而进行。The above treatment is performed by passing the tobacco supernatant through a column filled with particles containing inorganic porous bodies.
[A15]根据[A3]所述的方法，其中，[A15] The method according to [A3], wherein,
上述处理通过使上述有用成分溶出液通过填充有包含无机多孔体的粒子的柱而进行。The above-mentioned treatment is performed by passing the above-mentioned useful component eluate through a column filled with particles containing an inorganic porous body.
[A16]根据[A10]～[A15]中任一项所述的方法，其中，[A16] The method according to any one of [A10] to [A15], wherein,
上述粒子具有＜400μm、一般为10～400μm、优选为＜100μm、更优选为10～100μm、进一步优选为10～80μm、进一步优选为30～75μm的粒度。The above-mentioned particles have a particle size of <400 μm, generally 10-400 μm, preferably <100 μm, more preferably 10-100 μm, further preferably 10-80 μm, further preferably 30-75 μm.
[A17]根据[A1]～[A16]中任一项所述的方法，其中，[A17] The method according to any one of [A1] to [A16], wherein,
上述无机多孔体为硅藻土或沸石。The above-mentioned inorganic porous body is diatomite or zeolite.
[A18]根据[A10]～[A17]中任一项所述的方法，其中，[A18] The method according to any one of [A10] to [A17], wherein,
上述包含无机多孔体的粒子为仅由硅藻土形成的粒子或仅由沸石形成的粒子。The above-mentioned particles containing an inorganic porous body are particles formed only of diatomaceous earth or particles formed only of zeolite.
[A19]根据[A2]～[A18]中任一项所述的方法，其中，[A19] The method according to any one of [A2] to [A18], wherein,
上述烟草材料为烟丝。The above-mentioned tobacco material is cut tobacco.
[A20]根据[A2]～[A19]中任一项所述的方法，其中，[A20] The method according to any one of [A2] to [A19], wherein,
上述水性溶剂为水或含水乙醇，优选为水，更优选为20～70℃的水。The above-mentioned aqueous solvent is water or hydrous ethanol, preferably water, more preferably water at 20 to 70°C.
[A21]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A20]中任一项所述的方法，其中，[A21] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A20], wherein,
上述酵母为选自红酵母(Rhodotorula)属的酵母、法夫酵母(Xanthophyllomyces)属的酵母、耶氏酵母(Yarrowia)属的酵母、油脂酵母(Lipomyces)属的酵母、酵母菌(Saccharomyces)属的酵母、塞伯林德纳氏酵(Cyberlindnera)属的酵母、以及威克汉姆酵母(Wickerhamomyces)属的酵母中的至少1种酵母。The above-mentioned yeast is selected from the group consisting of yeast of the genus Rhodotorula, yeast of the genus Xanthophyllomyces, yeast of the genus Yarrowia, yeast of the genus Lipomyces, yeast of the genus Saccharomyces At least one kind of yeast among yeast, yeast of the genus Cyberlindnera, and yeast of the genus Wickerhamomyces.
[A22]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A21]中任一项所述的方法，其中，[A22] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A21], wherein,
上述溶出溶剂为有机溶剂、或有机溶剂与水的混合物。The above-mentioned dissolution solvent is an organic solvent or a mixture of an organic solvent and water.
[A23]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A22]中任一项所述的方法，其中，[A23] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A22], wherein,
上述有机溶剂为与水具有混和性的有机溶剂，优选为与水具有混和性的醇。The above-mentioned organic solvent is an organic solvent that is miscible with water, and is preferably an alcohol that is miscible with water.
[A24]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A23]中任一项所述的方法，其中，[A24] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A23], wherein,
上述溶出溶剂为与水具有混和性的醇、或其含水醇。The above-mentioned dissolution solvent is an alcohol that is miscible with water, or a water-containing alcohol thereof.
[A25]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A24]中任一项所述的方法，其中，[A25] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A24], wherein,
上述有机溶剂为具有10～14.5的SP值的有机溶剂，优选为具有10～14.5的SP值的醇，更优选为乙醇、异丙醇、甲醇或丁醇，进一步优选为乙醇或异丙醇，最优选为乙醇。The above-mentioned organic solvent is an organic solvent having an SP value of 10 to 14.5, preferably an alcohol having an SP value of 10 to 14.5, more preferably ethanol, isopropyl alcohol, methanol or butanol, further preferably ethanol or isopropyl alcohol, Most preferred is ethanol.
[A26]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A25]中任一项所述的方法，其中，[A26] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A25], wherein,
上述溶出溶剂为乙醇或含水乙醇。The above-mentioned dissolution solvent is ethanol or aqueous ethanol.
[A27]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A26]中任一项所述的方法，其中，[A27] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A26], wherein,
上述溶出溶剂以上述含酵母培养液与上述溶出溶剂的混合液中的上述有机溶剂的浓度达到50体积％以上、优选达到50～95体积％、更优选达到60～95体积％的量添加于上述含酵母培养液中。The above-mentioned dissolution solvent is added to the above-mentioned liquid in an amount such that the concentration of the above-mentioned organic solvent in the mixture of the above-mentioned yeast-containing culture solution and the above-mentioned dissolution solvent reaches 50% by volume or more, preferably 50-95% by volume, and more preferably 60-95% by volume. containing yeast culture medium.
[A28]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A27]中任一项所述的方法，其中，[A28] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A27], wherein,
上述溶出通过对上述含酵母培养液与上述溶出溶剂的混合物进行搅拌而进行。The above-mentioned dissolution is performed by stirring the mixture of the above-mentioned yeast-containing culture liquid and the above-mentioned dissolution solvent.
[A29]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A28]中任一项所述的方法，其中，[A29] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A28], wherein,
上述溶出通过对上述含酵母培养液与上述溶出溶剂的混合物进行加热而进行。The above-mentioned dissolution is performed by heating the mixture of the above-mentioned yeast-containing culture solution and the above-mentioned dissolution solvent.
[A30]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A29]中任一项所述的方法，该方法进一步包括：[A30] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A29], further comprising:
将上述有机溶剂从上述无微生物的有用成分溶出液中去除。The above-mentioned organic solvent is removed from the above-mentioned microorganism-free useful component eluate.
[A31]根据[A3]、[A6]、[A9]、[A12]、[A15]～[A30]中任一项所述的方法，其中，[A31] The method according to any one of [A3], [A6], [A9], [A12], [A15] to [A30], wherein,
上述有用成分为香味贡献成分。The above-mentioned useful ingredients are flavor-contributing ingredients.
[A32]根据[A31]所述的方法，其中，[A32] The method according to [A31], wherein,
上述香味贡献成分为选自类胡萝卜素、脂肪酸、中性脂肪(即，脂肪酸的甘油酯)、乙酸酯、脂肪酸酯、有机酸、以及高级醇(例如，碳原子数8～22的醇)中的至少1种成分。The aroma-contributing component is selected from the group consisting of carotenoids, fatty acids, neutral fats (ie, glyceryl esters of fatty acids), acetates, fatty acid esters, organic acids, and higher alcohols (for example, alcohols with 8 to 22 carbon atoms). ) at least 1 ingredient in.
[B1]一种烟草香味液，其是通过[A1]～[A32]中任一项所述的方法而得到的。[B1] A tobacco flavor liquid obtained by the method described in any one of [A1] to [A32].
[B2]根据[B1]所述的烟草香味液，其中，[B2] The tobacco flavor liquid according to [B1], wherein,
上述烟草香味液为[A2]所述的无微生物的烟草上清。The above-mentioned tobacco flavor liquid is the microorganism-free tobacco supernatant described in [A2].
[B3]根据[B1]所述的烟草香味液，其中，[B3] The tobacco flavor liquid according to [B1], wherein,
上述烟草香味液为[A3]所述的无微生物的有用成分溶出液。The above-mentioned tobacco flavor liquid is the microorganism-free useful component eluate described in [A3].
[C1]一种烟草添加物，其包含：[C1] A tobacco additive containing:
通过[A2]～[A32]中任一项所述的方法得到的烟草香味液、和Tobacco flavor liquid obtained by the method described in any one of [A2] to [A32], and
在[A2]～[A32]中任一项所述的方法中得到上述烟草上清时得到的烟草残渣。The tobacco residue obtained when the tobacco supernatant is obtained by the method according to any one of [A2] to [A32].
[C2]根据[C1]所述的烟草添加物，其中，[C2] The tobacco additive according to [C1], wherein,
上述烟草香味液为[A2]所述的无微生物的烟草上清。The above-mentioned tobacco flavor liquid is the microorganism-free tobacco supernatant described in [A2].
[C3]根据[C1]所述的烟草添加物，其中，[C3] The tobacco additive according to [C1], wherein,
上述烟草香味液为[A3]所述的无微生物的有用成分溶出液。The above-mentioned tobacco flavor liquid is the microorganism-free useful component eluate described in [A3].
[D1]一种香味抽吸器，其包含[B1]～[B3]中任一项所述的烟草香味液。[D1] A flavor inhaler containing the tobacco flavor liquid according to any one of [B1] to [B3].
[D2]一种香味抽吸器，其包含[C1]～[C3]中任一项所述的烟草添加物。[D2] A flavor inhaler containing the tobacco additive according to any one of [C1] to [C3].
[D3]根据[D1]或[D2]所述的香味抽吸器，其中，[D3] The fragrance inhaler according to [D1] or [D2], wherein,
上述香味抽吸器为燃烧型香味抽吸器。The above-mentioned fragrance inhaler is a combustion type fragrance inhaler.
[D4]根据[D1]或[D2]所述的香味抽吸器，其中，[D4] The fragrance inhaler according to [D1] or [D2], wherein,
上述香味抽吸器为加热型香味抽吸器。The above-mentioned fragrance inhaler is a heating type fragrance inhaler.
实施例Example
[实施例1][Example 1]
在实施例1中，对按照本发明的方法制造的烟草香味液的澄清性进行了评价。In Example 1, the clarity of the tobacco flavor liquid produced according to the method of the present invention was evaluated.
1-1.方法1-1.Method
将黄色种的烟叶粉碎，作为“烟草材料”使用。用粉碎器将黄色种的烟叶的丝(100g)粉碎至100μm以下的尺寸，添加60℃的水600mL，进行振荡(200rpm/2小时)。由此，提取出烟叶中所含的水溶性成分。然后，通过抽滤将烟草上清与烟草残渣分离。作为滤纸，使用了AdvantecNo.60。将得到的滤液称为“烟草上清”。The yellow tobacco leaves are crushed and used as "tobacco material". The shredded yellow tobacco leaves (100 g) were ground into a size of 100 μm or less using a grinder, and 600 mL of water at 60° C. was added and shaken (200 rpm/2 hours). Thereby, the water-soluble components contained in the tobacco leaves are extracted. Then, the tobacco supernatant and tobacco residue are separated by suction filtration. As filter paper, Advantec No. 60 was used. The obtained filtrate is called "tobacco supernatant".
对烟草上清进行以下的处理，制备了样品。The tobacco supernatant was processed as follows to prepare a sample.
样品1：Sample 1:
通过重力落下的方式使烟草上清5mL通过填充有粉状体的硅藻土(粒度：＜354μm)(GL Sciences公司)的柱(内径11mm及长度25mm)，得到了柱溶出液。将柱溶出液作为样品1。5 mL of the tobacco supernatant was allowed to pass through a column (inner diameter: 11 mm and length: 25 mm) filled with powdered diatomaceous earth (particle size: <354 μm) (GL Sciences) by gravity fall, and a column eluate was obtained. The column eluate was used as sample 1.
比较样品1：Comparison sample 1:
将烟草上清作为比较样品1。The tobacco supernatant was used as comparative sample 1.
参考样品1：Reference sample 1:
用孔径0.2μm的过滤器对烟草上清5mL进行了精密过滤。将得到的滤液作为参考样品1。5 mL of tobacco supernatant was precision filtered using a filter with a pore size of 0.2 μm. The obtained filtrate was used as reference sample 1.
样品2：Sample 2:
用高压釜对烟草上清100mL进行灭菌，以达到105细胞/mL的浓度的方式在灭菌后的烟草上清中添加酵母菌(Saccharomyces)属的酵母(酿酒酵母、Saccharomycescerevisiae)，振荡培养过夜(28℃、240rpm)。通过重力落下的方式使培养后得到的含酵母培养液5mL通过填充有粉状体的硅藻土(粒度：＜354μm)(GL Sciences公司)的柱(内径11mm及长度25mm)，得到了柱溶出液。将柱溶出液作为样品2。Sterilize 100 mL of the tobacco supernatant in an autoclave, add yeast of the genus Saccharomyces (Saccharomyces cerevisiae) to the sterilized tobacco supernatant so that the concentration reaches 105 cells/mL, and culture with shaking Overnight (28°C, 240rpm). The column dissolution was obtained by passing 5 mL of the yeast-containing culture solution obtained after cultivation through a column (inner diameter 11 mm and length 25 mm) filled with powdered diatomaceous earth (particle size: <354 μm) (GL Sciences) by falling by gravity. liquid. The column eluate was used as sample 2.
比较样品2：Comparison sample 2:
用高压釜对烟草上清100mL进行灭菌，以达到105细胞/mL的浓度的方式在灭菌后的烟草上清中添加酵母菌(Saccharomyces)属的酵母(酿酒酵母、Saccharomycescerevisiae)，振荡培养过夜(28℃、240rpm)。将培养后得到的含酵母培养液作为比较样品2。Sterilize 100 mL of the tobacco supernatant in an autoclave, add yeast of the genus Saccharomyces (Saccharomyces cerevisiae) to the sterilized tobacco supernatant so that the concentration reaches 105 cells/mL, and culture with shaking Overnight (28°C, 240rpm). The yeast-containing culture solution obtained after culturing was used as comparative sample 2.
参考样品2：Reference sample 2:
用高压釜对烟草上清100mL进行灭菌，以达到105细胞/mL的浓度的方式在灭菌后的烟草上清中添加酵母菌(Saccharomyces)属的酵母(酿酒酵母、Saccharomycescerevisiae)，振荡培养过夜(28℃、240rpm)。用孔径0.2μm的过滤器对培养后得到的含酵母培养液5mL进行了精密过滤。将得到的滤液作为参考样品2。Sterilize 100 mL of the tobacco supernatant in an autoclave, add yeast of the genus Saccharomyces (Saccharomyces cerevisiae) to the sterilized tobacco supernatant so that the concentration reaches 105 cells/mL, and culture with shaking Overnight (28°C, 240rpm). 5 mL of the yeast-containing culture solution obtained after the culture was precision filtered using a filter with a pore size of 0.2 μm. The obtained filtrate was used as reference sample 2.
对于各样品，通过使用吸光光度计测定600nm的吸光度，从而对澄清性进行了评价。作为样品1及比较样品1的澄清性的基准，使用了参考样品1。作为样品2及比较样品2的澄清性的基准，使用了参考样品2。For each sample, the clarity was evaluated by measuring the absorbance at 600 nm using an absorptiophotometer. As a benchmark for the clarity of Sample 1 and Comparative Sample 1, Reference Sample 1 was used. As a benchmark for the clarity of Sample 2 and Comparative Sample 2, Reference Sample 2 was used.
1-2.结果1-2. Results
将吸光度的测定结果示于以下的表中。The absorbance measurement results are shown in the table below.
[表1][Table 1]
表1所示的吸光度表示相对于参考样品的吸光度差。因此，在表1中，样品的吸光度的值越接近0，越表示具有与参考样品同等的澄清性。The absorbance shown in Table 1 represents the absorbance difference relative to the reference sample. Therefore, in Table 1, the closer the absorbance value of the sample is to 0, the closer it is to having the same clarity as the reference sample.
样品1的澄清性优于比较样品1。该结果表明，通过使烟草上清通过填充有硅藻土的柱，去除了使澄清性降低的分子量大的悬浮物(包括烟草上清中所含的微生物)。The clarity of sample 1 is better than that of comparative sample 1. This result shows that by passing the tobacco supernatant through a column filled with diatomaceous earth, large molecular weight suspended solids (including microorganisms contained in the tobacco supernatant) that reduce the clarity are removed.
样品2的澄清性优于比较样品2。该结果表明，通过使烟草上清通过填充有硅藻土的柱，去除了使澄清性降低的分子量大的悬浮物(包括烟草上清中所含的微生物及添加的酵母)。The clarity of sample 2 is better than that of comparative sample 2. This result shows that by passing the tobacco supernatant through a column filled with diatomaceous earth, large molecular weight suspended solids (including microorganisms contained in the tobacco supernatant and added yeast) that reduce the clarity are removed.
上述结果证实了通过本发明的方法制造的烟草香味液的澄清性，因此可以认为，根据本发明的方法，不仅能够将添加的酵母去除，还能够将烟草上清中所含的所有微生物、例如霉菌、细菌等也去除。The above results confirm the clarity of the tobacco flavor liquid produced by the method of the present invention. Therefore, it is considered that according to the method of the present invention, not only the added yeast but also all microorganisms contained in the tobacco supernatant, such as Mold, bacteria, etc. are also removed.
[实施例2][Example 2]
在实施例2中，对按照本发明的方法制造的烟草香味液的酵母浓度进行了评价。In Example 2, the yeast concentration of the tobacco flavor liquid produced according to the method of the present invention was evaluated.
2-1.方法2-1.Method
按照实施例1中记载的方法得到了烟草上清。对烟草上清进行以下的处理，制备了样品。Tobacco supernatant was obtained according to the method described in Example 1. The tobacco supernatant was processed as follows to prepare a sample.
样品3A：Sample 3A:
用高压釜对烟草上清100mL进行灭菌，以达到105细胞/mL的浓度的方式在灭菌后的烟草上清中添加酵母菌(Saccharomyces)属的酵母(酿酒酵母、Saccharomycescerevisiae)，振荡培养过夜(28℃、240rpm)。将培养后得到的含酵母培养液作为样品3A。Sterilize 100 mL of tobacco supernatant in an autoclave, add yeast of the genus Saccharomyces (Saccharomyces cerevisiae) to the sterilized tobacco supernatant so that the concentration reaches 105 cells/mL, and culture with shaking Overnight (28°C, 240rpm). The yeast-containing culture solution obtained after culturing was used as sample 3A.
样品3B：Sample 3B:
通过重力落下的方式使含酵母培养液(样品3A)5mL通过填充有玻璃粉末(粒度：63～106μm)(AS ONE公司)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品3B。5 mL of the yeast-containing culture solution (sample 3A) was allowed to pass through a column (inner diameter 11 mm and length 25 mm) filled with glass powder (particle size: 63 to 106 μm) (AS ONE Company) by gravity fall, to obtain a column eluate. This column eluate was used as sample 3B.
样品3C：Sample 3C:
通过重力落下的方式使含酵母培养液(样品3A)5mL通过填充有粉状体的硅藻土(粒度：＜105μm)(IMERYS)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品3C。5 mL of the yeast culture solution (sample 3A) was passed through a column (inner diameter: 11 mm and length: 25 mm) filled with powdered diatomaceous earth (particle size: <105 μm) (IMERYS) by falling by gravity to obtain a column eluate. This column eluate was used as sample 3C.
样品3D：Sample 3D:
通过重力落下的方式使含酵母培养液(样品3A)5mL通过填充有粉状体的硅藻土(粒度：＜50μm)(GL Sciences公司)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品3D。Column dissolution was obtained by passing 5 mL of a yeast culture solution (sample 3A) through a column (inner diameter 11 mm and length 25 mm) filled with powdered diatomaceous earth (particle size: <50 μm) (GL Sciences) by falling by gravity. liquid. This column eluate was used as sample 3D.
样品3E：Sample 3E:
通过重力落下的方式使含酵母培养液(样品3A)5mL通过填充有粉状体的硅藻土(粒度：＜354μm)(GL Sciences公司)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品3E。Column dissolution was obtained by passing 5 mL of yeast culture solution (sample 3A) through a column (inner diameter 11 mm and length 25 mm) filled with powdered diatomaceous earth (particle size: <354 μm) (GL Sciences) by gravity fall. liquid. This column eluate was used as sample 3E.
样品3F：Sample 3F:
通过重力落下的方式使含酵母培养液(样品3A)5mL通过填充有粉状体的合成沸石(粒度：＜75μm)(富士胶片和光纯药株式会社)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品3F。5 mL of a yeast-containing culture solution (sample 3A) was passed through a column (inner diameter 11 mm and length 25 mm) filled with powdery synthetic zeolite (particle size: <75 μm) (Fuji Film Wako Pure Chemical Industries, Ltd.) by gravity fall to obtain The column eluate was removed. This column eluate was used as sample 3F.
样品4A：Sample 4A:
另一方面，对含酵母培养液(样品3A)进行离心分离，得到了离心分离上清。将离心分离上清作为样品4A。On the other hand, the yeast-containing culture solution (sample 3A) was centrifuged to obtain a centrifugation supernatant. The supernatant was centrifuged and used as sample 4A.
样品4B：Sample 4B:
通过重力落下的方式使离心分离上清(样品4A)5mL通过填充有玻璃粉末(63～106μm的粒径)(AS ONE公司)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品4B。5 mL of the centrifugation supernatant (sample 4A) was allowed to pass through a column (inner diameter 11 mm and length 25 mm) filled with glass powder (particle diameter 63 to 106 μm) (AS ONE Company) by gravity fall, to obtain a column eluate. This column eluate was used as sample 4B.
样品4C：Sample 4C:
通过重力落下的方式使离心分离上清(样品4A)5mL通过填充有粉状体的硅藻土(粒度：＜105μm)(IMERYS)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品4C。5 mL of the centrifugation supernatant (sample 4A) was passed through a column (internal diameter: 11 mm and length: 25 mm) filled with powdered diatomaceous earth (particle size: <105 μm) (IMERYS) by gravity fall to obtain a column eluate. This column eluate was used as sample 4C.
样品4D：Sample 4D:
通过重力落下的方式使离心分离上清(样品4A)5mL通过填充有粉状体的硅藻土(粒度：＜50μm)(IMERYS)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品4D。5 mL of the centrifugation supernatant (sample 4A) was passed through a column (internal diameter 11 mm and length 25 mm) filled with powdered diatomaceous earth (particle size: <50 μm) (IMERYS) by gravity fall, and a column eluate was obtained. This column eluate was used as sample 4D.
样品4E：Sample 4E:
通过重力落下的方式使离心分离上清(样品4A)5mL通过填充有填充有粉状体的硅藻土(粒度：＜354μm)(GL Sciences公司)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品4E。5 mL of the centrifugation supernatant (sample 4A) was passed through a column (inner diameter 11 mm and length 25 mm) filled with powdered diatomaceous earth (particle size: <354 μm) (GL Sciences) by falling by gravity to obtain Column eluate. This column eluate was used as sample 4E.
样品4F：Sample 4F:
通过重力落下的方式使离心分离上清(样品4A)5mL通过填充有粉状体的合成沸石(粒度：＜75μm)(富士胶片和光纯药株式会社)的柱(内径11mm及长度25mm)，得到了柱溶出液。将该柱溶出液作为样品4F。5 mL of the centrifugation supernatant (sample 4A) was passed through a column (inner diameter 11 mm and length 25 mm) filled with powdered synthetic zeolite (particle size: <75 μm) (Fuji Film Wako Pure Chemical Industries, Ltd.) by falling by gravity to obtain The column eluate was removed. This column eluate was used as sample 4F.
通过菌落计数法对样品3A～3F、4A～4F中的酵母菌体数进行了测定。The number of yeast cells in samples 3A to 3F and 4A to 4F was measured by the colony counting method.
2-2.结果2-2. Results
将酵母菌体数的测定结果示于以下的表中。The measurement results of the yeast cell count are shown in the table below.
[表2][Table 2]
可以确认，在使烟草上清通过了硅藻土填充柱的情况下，酵母已被可靠地去除。即使改变硅藻土的粒度，也能够同样地将酵母去除。另外，可以确认，在使烟草上清通过了沸石填充柱的情况下，酵母也已被可靠地去除。另一方面，在使烟草上清通过了玻璃粉末填充柱的情况下，无法将酵母去除。It was confirmed that yeast was reliably removed when the tobacco supernatant was passed through the diatomaceous earth packed column. Even if the particle size of diatomaceous earth is changed, yeast can be removed in the same way. In addition, it was confirmed that yeast was reliably removed even when the tobacco supernatant was passed through the zeolite packed column. On the other hand, when the tobacco supernatant was passed through a glass powder packed column, yeast could not be removed.
该结果表明，如果按照本发明的方法用无机多孔体对烟草上清进行处理，则能够将添加的酵母去除至无法检测的水平。根据该结果和实施例1的结果可以认为，本发明的方法除了能够将添加的酵母去除以外，还能够将烟草上清中所含的所有微生物、例如霉菌、细菌等也去除。This result shows that if the tobacco supernatant is treated with an inorganic porous body according to the method of the present invention, the added yeast can be removed to an undetectable level. Based on this result and the result of Example 1, it is considered that the method of the present invention can remove not only the added yeast but also all microorganisms, such as molds and bacteria, contained in the tobacco supernatant.
[实施例3][Example 3]
在实施例3中，实施了第2实施方式的方法。In Example 3, the method of the second embodiment was implemented.
3-1.方法3-1.Method
样品5：Sample 5:
按照第2实施方式的方法进行提取工序(S1)、培养工序(S2)及溶出工序(S3)，通过重力落下的方式使得到的有用成分溶出液5mL通过填充有粉状体的硅藻土(粒度：＜354μm)(GL Sciences公司)的柱(内径11mm及长度25mm)。将柱溶出液作为样品5。The extraction step (S1), the culture step (S2) and the dissolution step (S3) were carried out according to the method of the second embodiment, and 5 mL of the obtained useful component eluate was passed through diatomaceous earth filled with powder by falling by gravity. Particle size: <354 μm) (GL Sciences) column (inner diameter 11 mm and length 25 mm). The column eluate was used as sample 5.
比较样品5：Comparison sample 5:
按照第2实施方式的方法进行提取工序(S1)、培养工序(S2)及溶出工序(S3)，将得到的有用成分溶出液作为比较样品5。The extraction step (S1), the culture step (S2), and the elution step (S3) were performed according to the method of the second embodiment, and the obtained useful component eluate was used as comparative sample 5.
参考样品5：Reference sample 5:
按照第2实施方式的方法进行提取工序(S1)、培养工序(S2)及溶出工序(S3)，通过孔径0.2μm的过滤器对得到的有用成分溶出液5mL进行了精密过滤。将得到的滤液作为参考样品5。The extraction step (S1), the culture step (S2), and the elution step (S3) were performed according to the method of the second embodiment, and 5 mL of the obtained useful component eluate was precision filtered through a filter with a pore size of 0.2 μm. The obtained filtrate was used as reference sample 5.
以下记载提取工序(S1)、培养工序(S2)及溶出工序(S3)的详细情况。The details of the extraction step (S1), the culture step (S2), and the dissolution step (S3) are described below.
提取工序(S1)Extraction process (S1)
将黄色种的烟叶粉碎，作为“烟草材料”使用。用粉碎器将黄色种的烟叶的丝(100g)粉碎至100μm以下的尺寸，添加60℃的水600mL，进行振荡(200rpm/2小时)。由此，提取出烟叶中所含的水溶性成分。然后，通过过滤进行了固液分离。由此得到了烟草上清及烟草残渣。The yellow tobacco leaves are crushed and used as "tobacco material". The shredded yellow tobacco leaves (100 g) were ground into a size of 100 μm or less using a grinder, and 600 mL of water at 60° C. was added and shaken (200 rpm/2 hours). Thereby, the water-soluble components contained in the tobacco leaves are extracted. Then, solid-liquid separation was performed by filtration. Thus, tobacco supernatant and tobacco residue were obtained.
培养工序(S2)Culture process (S2)
以达到105细胞/mL的浓度的方式在得到的烟草上清3mL中添加酵母菌(Saccharomyces)属的酵母(酿酒酵母、Saccharomyces cerevisiae)，在烟草上清中对酵母进行了培养。培养通过在需氧条件下以28℃实施振荡培养(240rpm)24小时而进行。将培养后得到的“酵母与烟草上清的混合物”称为“含酵母培养液”。Yeast (Saccharomyces cerevisiae) of the genus Saccharomyces was added to 3 mL of the obtained tobacco supernatant so as to reach a concentration of 105 cells/mL, and the yeast was cultured in the tobacco supernatant. The culture was performed by performing shaking culture (240 rpm) at 28° C. for 24 hours under aerobic conditions. The "mixture of yeast and tobacco supernatant" obtained after cultivation is called "yeast-containing culture solution".
溶出工序(S3)Dissolution process (S3)
在开始培养24小时后，在含酵母培养液3mL中添加了作为溶出溶剂的乙醇7mL。具体而言，以使含酵母培养液与乙醇的混合液中的乙醇的浓度达到70体积％的量添加了乙醇。24 hours after starting the culture, 7 mL of ethanol as an elution solvent was added to 3 mL of the yeast-containing culture solution. Specifically, ethanol was added in an amount such that the concentration of ethanol in the mixed solution containing the yeast culture solution and ethanol reaches 70% by volume.
对于各样品，通过使用吸光光度计测定600nm的吸光度，从而对澄清性进行了评价。作为样品5及比较样品5的澄清性的基准，使用了参考样品5。For each sample, the clarity was evaluated by measuring the absorbance at 600 nm using an absorptiophotometer. As a benchmark for the clarity of Sample 5 and Comparative Sample 5, Reference Sample 5 was used.
3-2.结果3-2. Results
将吸光度的测定结果示于以下的表中。The absorbance measurement results are shown in the table below.
[表3][table 3]
表3所示的吸光度表示相对于参考样品5的吸光度差。因此，在表3中，样品的吸光度的值越接近0，越表示具有与参考样品5同等的澄清性。The absorbance shown in Table 3 represents the absorbance difference relative to the reference sample 5. Therefore, in Table 3, the closer the absorbance value of the sample is to 0, the more clearly it has the same clarity as the reference sample 5.
样品5的澄清性优于比较样品5。该结果表明，通过使按照第2实施方式的方法得到的有用成分溶出液通过填充有硅藻土的柱，去除了使澄清性降低的分子量大的悬浮物(包括烟草上清中所含的微生物及添加的酵母)。The clarity of sample 5 is better than that of comparative sample 5. This result shows that by passing the useful component eluate obtained by the method of the second embodiment through a column filled with diatomaceous earth, large molecular weight suspended solids (including microorganisms contained in the tobacco supernatant) that reduce the clarity are removed. and added yeast).
[实施例4][Example 4]
在实施例4中进行了感官评价。In Example 4, sensory evaluation was performed.
4-1.方法4-1.Method
如实施例1的记载所述制备了样品1、比较样品1、参考样品1、样品2、比较样品2、参考样品2，作为烟草香味液使用。将各样品1mL与丙二醇1mL混合，使用市售的电子烟进行雾化/抽吸，对其香吸味进行了评价。Sample 1, comparative sample 1, reference sample 1, sample 2, comparative sample 2, and reference sample 2 were prepared as described in Example 1 and used as tobacco flavor liquid. 1 mL of each sample was mixed with 1 mL of propylene glycol, atomized/smoked using a commercially available electronic cigarette, and the aroma was evaluated.
4-2.结果4-2. Results
在样品1与比较样品1之间无法检测出香吸味的差异。另外，在样品2与比较样品2之间无法检测出香吸味的差异。这些结果表明，在使烟草上清通过了硅藻土柱的情况下，虽然分子量大的悬浮物被去除，但对香吸味造成影响的低分子化合物并未被去除，该处理工序不会对烟草上清的香吸味造成影响。No difference in aroma was detectable between Sample 1 and Comparative Sample 1. In addition, the difference in the aroma between Sample 2 and Comparative Sample 2 could not be detected. These results show that when the tobacco supernatant is passed through a diatomaceous earth column, although the suspended solids with large molecular weight are removed, the low-molecular compounds that affect the aroma are not removed, and this treatment process will not affect the aroma. The aroma of the tobacco supernatant has an impact.
在样品1与参考样品1之间无法检测出香吸味的差异。另外，在样品2与参考样品2之间无法检测出香吸味的差异。这些结果表明，在通过了硅藻土柱的情况与进行了精密过滤的情况之间，得到的烟草香味液的香吸味没有差别。No difference in aroma was detectable between Sample 1 and Reference Sample 1. In addition, no difference in aroma was detected between Sample 2 and Reference Sample 2. These results show that there is no difference in the aroma of the tobacco flavor liquid obtained between the case where the liquid passed through the diatomaceous earth column and the case where precision filtration was performed.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/JP2021/019623WO2022249242A1 (en) | 2021-05-24 | 2021-05-24 | Tobacco-flavored liquid manufacturing method, tobacco-flavored liquid, and flavor inhaler |
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|---|---|
| CN117425413Atrue CN117425413A (en) | 2024-01-19 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202180098113.8APendingCN117425413A (en) | 2021-05-24 | 2021-05-24 | Method for producing tobacco flavor liquid, and flavor aspirator |
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| EP (1) | EP4349185A4 (en) |
| JP (1) | JP7574436B2 (en) |
| KR (1) | KR20230158090A (en) |
| CN (1) | CN117425413A (en) |
| WO (1) | WO2022249242A1 (en) |
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| JP7574436B2 (en) | 2024-10-28 |
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