技术领域Technical field
本申请涉及试剂检测技术领域，特别涉及一种微流控联检卡壳及装置。The present application relates to the technical field of reagent detection, and in particular to a microfluidic joint detection cartridge and device.
背景技术Background technique
相关技术中，联检检测卡壳的每个检测项对应一个加样孔，每次加样只能完成一个项目检测，或单个加样孔无法做到联检项目样本的均匀分配和保证联检项目同时检测，检测操作繁琐。不能同时加样和样本的不均匀分配容易导致样本差异影响试纸检测结果，降低检测准确性。In the related technology, each detection item of the joint inspection stuck corresponds to a sampling hole. Each sample addition can only complete the detection of one item, or a single sampling hole cannot evenly distribute the samples of the joint inspection items and ensure the simultaneous testing of the joint inspection items. The detection operation is cumbersome. Failure to add samples at the same time and uneven distribution of samples can easily lead to sample differences that affect test paper test results and reduce test accuracy.
在一种多联检测卡中，加样孔底部适配储样槽，储样槽适配导流管，导流管末端与试纸条接触，该方案可满足多项目联检，但加样操作和导流管长短差异易造成样本分配不均匀以及试纸条上样时间差异导致联检项目结果误差且毛细作用中导流管大小限制液体流动时间，难以在实际运用中实施。在另一种联合检测卡中，该检测卡将TORCH五项目IgM测试整合至一个检测卡上，每个项目对应一个加样孔及一个结果观察窗，五项目IgM层析检测试纸不能满足对用户感染状况充分解读，且该方案五个加样孔导致加样操作繁琐，易造成加样时间误差导致样本差异判断结果偏移。In a multi-link detection card, the bottom of the sampling hole is adapted to the sample storage tank, the sample storage tank is adapted to the guide tube, and the end of the guide tube is in contact with the test strip. This solution can meet the requirements of multi-item joint inspection, but the sample addition operation The difference in length and length of the guide tube can easily lead to uneven distribution of samples and differences in the loading time of test strips, resulting in errors in the results of joint inspection projects. The size of the guide tube limits the liquid flow time during capillary action, making it difficult to implement in actual applications. In another joint test card, this test card integrates TORCH's five-item IgM test into one test card. Each item corresponds to a sampling hole and a result observation window. The five-item IgM chromatography test paper cannot satisfy the needs of users. The infection status can be fully interpreted, and the five sampling holes in this scheme make the sampling operation cumbersome, which can easily cause errors in the sampling time and lead to biased sample difference judgment results.
发明内容Contents of the invention
本申请旨在至少解决现有技术中存在的技术问题之一。为此，本申请提出一种微流控联检装置，能够实现多个试纸同时开始检测，同时减低由检测操作及判读导致的检测结果误差。This application aims to solve at least one of the technical problems existing in the prior art. To this end, this application proposes a microfluidic joint detection device that can enable multiple test strips to start testing at the same time, while reducing detection result errors caused by detection operations and interpretation.
本申请还提出一种具有上述微流控联检卡壳的微流控联检装置。This application also proposes a microfluidic joint detection device with the above-mentioned microfluidic joint detection stuck housing.
本申请一方面实施例的微流控联检卡壳，包括底板和盖板，所述盖板与所述底板配合连接；所述底板具有阶梯结构、储液槽、导流渠及上样区，所述阶梯结构呈弧线状分布，所述阶梯结构与所述盖板之间形成有毛细层，所述储液槽和所述导流渠位于所述阶梯结构的两侧，所述导流渠的一端与所述阶梯结构连接，所述上样区与所述导流渠的另一端连通，所述导流渠和所述上样区均具有多个；所述盖板设置有第一导流通道，所述第一导流通道位于所述导流渠的上方，且所述第一导流通道沿垂直于所述盖板方向的投影至少部分位于所述阶梯结构上。The microfluidic joint detection cartridge according to one aspect of the present application includes a bottom plate and a cover plate, and the cover plate is cooperatively connected with the bottom plate; the bottom plate has a ladder structure, a liquid storage tank, a diversion channel and a sample loading area. The ladder structure is distributed in an arc shape, and a capillary layer is formed between the ladder structure and the cover plate. The liquid storage tank and the diversion channel are located on both sides of the ladder structure, and the diversion channel One end is connected to the ladder structure, the sample loading area is connected to the other end of the diversion channel, and both the diversion channel and the sample loading area have multiple; the cover plate is provided with a first guide The first flow channel is located above the flow channel, and the projection of the first flow channel in a direction perpendicular to the cover plate is at least partially located on the stepped structure.
进一步地，所述阶梯结构包括第一阶梯，所述第一阶梯具有第一端面，所述第一端面与所述储液槽底部的距离为0.3～1.0mm。Further, the ladder structure includes a first step, the first step has a first end face, and the distance between the first end face and the bottom of the liquid storage tank is 0.3 to 1.0 mm.
进一步地，所述阶梯结构还包括第二阶梯，所述第二阶梯具有第二端面，所述第二端面与所述第一端面的间距为0.3～1.0mm。Furthermore, the ladder structure further includes a second step, the second step has a second end face, and a distance between the second end face and the first end face is 0.3 to 1.0 mm.
进一步地，所述第二端面与所述盖板限定出所述毛细层，所述毛细层的高度为0.3-0.6mm。Further, the second end surface and the cover plate define the capillary layer, and the height of the capillary layer is 0.3-0.6 mm.
进一步地，所述导流渠的长度为3～6mm。Further, the length of the diversion channel is 3 to 6 mm.
进一步地，所述盖板还设置有第一凸起，所述第一导流通道至少部分与所述第一凸起连接，且所述第一凸起沿竖直方向的投影位于所述上样区内。Further, the cover plate is also provided with a first protrusion, the first guide channel is at least partially connected to the first protrusion, and the projection of the first protrusion in the vertical direction is located on the upper within the sample area.
进一步地，所述第一导流通道贯穿所述第一凸起设置。Further, the first flow guide channel is disposed through the first protrusion.
进一步地，沿垂直于所述盖板方向，所述第一凸起凸出于所述第一导流通道0.2～0.5mm设置。Further, in a direction perpendicular to the cover plate, the first protrusion protrudes from the first flow guide channel by 0.2 to 0.5 mm.
进一步地，所述底板设置有第二导流通道，所述第二导流通道的一端与所述储液槽连通，所述第二导流通道的另一端延伸至所述阶梯结构的端面上。Further, the bottom plate is provided with a second flow guide channel, one end of the second flow guide channel is connected to the liquid storage tank, and the other end of the second flow guide channel extends to the end surface of the ladder structure. .
进一步地，所述盖板具有加样孔，所述加样孔的位置与所述储液槽对应，所述盖板还设置有第二凸起，所述第二凸起围绕所述加样孔并呈放射状设置。Further, the cover plate has a sample adding hole, and the position of the sample adding hole corresponds to the liquid storage tank. The cover plate is also provided with a second protrusion, and the second protrusion surrounds the sample adding hole. The holes are arranged radially.
进一步地，沿靠近所述加样孔方向，所述第二凸起的高度逐渐减小。Further, the height of the second protrusion gradually decreases along the direction approaching the sampling hole.
本申请另一方面实施例的一种微流控联检装置，包括如前所述的微流控联检卡壳。A microfluidic joint detection device according to another embodiment of the present application includes the microfluidic joint detection cartridge as described above.
前述的微流控联检卡壳及微流控联检装置，至少具有如下有益效果：检测时，检测试纸的上样部放置于上样区内，储液槽内的待检测样本经阶梯结构、第一导流通道及导流渠后到达上样区，并与位于上样区的检测试纸的上样部接触以完成上样。阶梯结构位于储液槽的一侧，且阶梯结构靠近储液槽的一侧呈弧线状，使得储液槽内的待检测样本达到各个第一导流通道或导流渠的距离相等，利于使储液槽内的待检测样本到达各个检测试纸的时间保持一致，利于储液槽中的待检测样本能够均匀分配到各个上样区中，同时也使各个检测试纸能够同时开始检测；待检测样本在储液槽内混匀后再同一分配到各个上样区，避免了分次加样时存在的样本差异、多个被测样本差异和检测时间差异等因素造成的检测误差和结果偏移，同时也提高了用户便捷操作的体验度；阶梯结构与盖板之间形成有毛细层，样本量超过储液槽容积时毛细层起毛细作用将液体平均分配至各导流渠入口处，同时盖板中设置有第一导流通道，入口处的液体在第一导流通道的毛细作用下自驱动引导进入上样区。在第一导流通道及毛细层的作用下，储液槽中的待检测样本能够被均匀地、自驱动地引导至上样区，利于降低加样操作带来的误差；此外，第一导流通道与导流渠能够引导待检测样本流向上样区，导流渠的横截面积较大，相较于仅设置第一导流通道能够输送更多的待检测样本，利于缩短上样时间。The aforementioned microfluidic joint detection cartridge and microfluidic joint detection device have at least the following beneficial effects: during detection, the sample loading part of the detection test paper is placed in the sample loading area, and the sample to be detected in the liquid storage tank passes through the ladder structure, the first The diversion channel and the diversion channel then reach the sample loading area, and come into contact with the sample loading part of the test paper located in the sample loading area to complete the sample loading. The ladder structure is located on one side of the liquid storage tank, and the side of the ladder structure close to the liquid storage tank is arc-shaped, so that the distance between the samples to be detected in the liquid storage tank and each first diversion channel or diversion channel is equal, which is beneficial to Keep the time when the samples to be tested in the liquid storage tank arrive at each test paper consistent, which will help the samples to be tested in the liquid storage tank to be evenly distributed to each sample loading area, and also enable each test paper to start testing at the same time; to be tested The samples are mixed in the storage tank and then uniformly distributed to each loading area, which avoids detection errors and result offsets caused by sample differences during divided sampling, differences in multiple tested samples, and differences in detection time. , and also improves the user's convenient operation experience; a capillary layer is formed between the ladder structure and the cover plate. When the sample volume exceeds the volume of the reservoir, the capillary layer acts as a capillary to evenly distribute the liquid to the entrance of each diversion channel. At the same time, A first guide channel is provided in the cover plate, and the liquid at the inlet is self-driven and guided into the sample loading area under the capillary action of the first guide channel. Under the action of the first flow guide channel and the capillary layer, the sample to be detected in the liquid storage tank can be evenly and self-drivenly guided to the sample loading area, which is beneficial to reducing errors caused by the sample addition operation; in addition, the first flow guide The channel and the diversion channel can guide the sample to be detected to flow to the sample loading area. The cross-sectional area of the diversion channel is larger, which can transport more samples to be detected compared with only providing the first diversion channel, which is beneficial to shortening the sample loading time.
本申请的附加方面和优点将在下面的描述中部分给出，部分将从下面的描述中变得明显，或通过本申请的实践了解到。Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.
附图说明Description of the drawings
下面结合附图和实施例对本申请做进一步的说明，其中：The present application will be further described below in conjunction with the accompanying drawings and examples, wherein:
图1为本申请一方面实施例的微流控联检卡壳的整体结构示意图；Figure 1 is a schematic diagram of the overall structure of a microfluidic joint detection cartridge according to an embodiment of the present application;
图2为本申请一方面实施例的微流控联检卡壳的俯视示意图；Figure 2 is a schematic top view of the microfluidic joint detection cartridge according to one embodiment of the present application;
图3为图2中A-A的剖视示意图；Figure 3 is a schematic cross-sectional view of A-A in Figure 2;
图4为图3中B部分的局部放大示意图；Figure 4 is a partially enlarged schematic diagram of part B in Figure 3;
图5为本申请一方面实施例的微流控联检卡壳中底板的结构示意图；Figure 5 is a schematic structural diagram of the bottom plate of the microfluidic joint detection cartridge according to an embodiment of the present application;
图6为本申请一方面实施例的微流控联检卡壳中底板的俯视示意图；Figure 6 is a schematic top view of the bottom plate of the microfluidic joint detection cartridge according to an embodiment of the present application;
图7为图6中C-C的剖视示意图；Figure 7 is a schematic cross-sectional view of C-C in Figure 6;
图8为图7中D部分的局部放大示意图；Figure 8 is a partially enlarged schematic diagram of part D in Figure 7;
图9为本申请一方面实施例的盖板背面的结构示意图。FIG. 9 is a schematic structural diagram of the back side of the cover plate according to an embodiment of the present application.
附图标记：Reference signs:
100、底板；110、阶梯结构；111、第一阶梯；1111、第一端面；112、第二阶梯；1121、第二端面；120、储液槽；130、导流渠；140、上样区；150、第三凸起；160、第二导流通道；170、放置区；180、连接柱；100. Bottom plate; 110. Ladder structure; 111. First step; 1111. First end face; 112. Second step; 1121. Second end face; 120. Liquid storage tank; 130. Diversion channel; 140. Sample loading area ; 150. Third protrusion; 160. Second diversion channel; 170. Placement area; 180. Connecting column;
200、盖板；210、加样孔；220、观察窗；230、第一导流通道；240、第一凸起；250、第二凸起。200. Cover plate; 210. Sampling hole; 220. Observation window; 230. First diversion channel; 240. First protrusion; 250. Second protrusion.
具体实施方式Detailed ways
下面详细描述本申请的实施例，所述实施例的示例在附图中示出，其中自始至终相同或类似的标号表示相同或类似的元件或具有相同或类似功能的元件。下面通过参考附图描述的实施例是示例性的，仅用于解释本申请，而不能理解为对本申请的限制。The embodiments of the present application are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements with the same or similar functions. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present application and cannot be understood as limiting the present application.
在本申请的描述中，需要理解的是，涉及到方位描述，例如上、下、前、后、左、右等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本申请和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本申请的限制。In the description of this application, it should be understood that the orientation descriptions involved, such as the orientation or positional relationship indicated by up, down, front, back, left, right, etc. are based on the orientation or positional relationship shown in the drawings, and are only In order to facilitate the description of the present application and simplify the description, it is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation of the present application.
在本申请的描述中，若干的含义是一个以上，多个的含义是两个以上，大于、小于、超过等理解为不包括本数，以上、以下、以内等理解为包括本数。如果有描述到第一、第二只是用于区分技术特征为目的，而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量或者隐含指明所指示的技术特征的先后关系。In the description of this application, several means one or more, plural means two or more, greater than, less than, exceeding, etc. are understood to exclude the original number, and above, below, within, etc. are understood to include the original number. If there is a description of first and second, it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the order of indicated technical features. relation.
本申请的描述中，除非另有明确的限定，设置、安装、连接等词语应做广义理解，所属技术领域技术人员可以结合技术方案的具体内容合理确定上述词语在本申请中的具体含义。In the description of this application, unless otherwise explicitly limited, words such as setting, installation, and connection should be understood in a broad sense. Those skilled in the art can reasonably determine the specific meaning of the above words in this application in conjunction with the specific content of the technical solution.
本申请的描述中，参考术语“一个实施例”、“一些实施例”、“示意性实施例”、“示例”、“具体示例”、或“一些示例”等的描述意指结合该实施例或示例描述的具体特征、结构、材料或者特点包含于本申请的至少一个实施例或示例中。在本说明书中，对上述术语的示意性表述不一定指的是相同的实施例或示例。而且，描述的具体特征、结构、材料或者特点可以在任何的一个或多个实施例或示例中以合适的方式结合。In the description of this application, reference to the description of the terms "one embodiment," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" is intended to be in conjunction with the description of the embodiment. or examples describe specific features, structures, materials, or characteristics that are included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
本申请一方面实施例公开的一种微流控联检装置，包括微流控联检卡壳和检测试纸，微流控联检卡壳包括底板100，底板100设置有储液槽120、上样区140和多个用于放置检测试纸的放置区170，检测试纸放置于放置区170中，且检测试纸的上样部位于上样区140。检测时，储液槽120内的待检测样本流至上样区140后，与位于上样区140上样部接触，进而实现上样并开始检测。An embodiment of the present application discloses a microfluidic joint detection device, which includes a microfluidic joint detection cartridge and detection test strips. The microfluidic joint detection cartridge includes a bottom plate 100, and the bottom plate 100 is provided with a liquid storage tank 120, a sample loading area 140 and multiple There is a placement area 170 for placing detection test paper, the detection test paper is placed in the placement area 170, and the sample loading part of the detection test paper is located in the sample loading area 140. During detection, the sample to be detected in the liquid storage tank 120 flows to the sample loading area 140 and contacts the sample loading part of the sample loading area 140, thereby loading the sample and starting detection.
应当指出，本申请实施例的微流控联检装置能够适用于包括但不限于TORCH IgG、IgM十项、TORCHIgM五项目、和传染病四项的检测工作中。It should be noted that the microfluidic joint detection device of the embodiment of the present application can be applied to detection work including but not limited to TORCH IgG, ten IgM items, five TORC IgM items, and four infectious diseases.
本申请另一方面实施例公开了一种微流控联检卡壳，如图1至图4所示，该微流控联检卡壳包括底板100和盖板200，盖板200与底板100配合连接。其中，底板100具有阶梯结构110、储液槽120、导流渠130及上样区140，阶梯结构110呈弧线状分布，阶梯结构110与盖板200之间形成有毛细层，储液槽120和导流渠130位于阶梯结构110的两侧，导流渠130的一端与阶梯结构110连接，上样区140与导流渠130的另一端连通，导流渠130和上样区140均具有多个；盖板200设置有第一导流通道230，第一导流通道230位于导流渠130的上方，且第一导流通道230沿垂直于盖板200方向的投影至少部分位于阶梯结构110上。其中，第一导流通道230为毛细通道，能够驱动待检测样本从阶梯结构110一侧流向上样区140一侧。毛细层对待检测样本产生毛细作用力，可以驱动待检测样本定向移动。毛细层形成于阶梯结构110与盖板200之间，毛细层对待检测样本的毛细作用力有限，因此，第一导流通道230的设置，能够进一步带动导流渠130中的待检测样本朝向上样区140一侧移动。Another embodiment of the present application discloses a microfluidic joint detection cartridge. As shown in FIGS. 1 to 4 , the microfluidic joint detection cartridge includes a base plate 100 and a cover plate 200 . The cover plate 200 is cooperatively connected with the base plate 100 . Among them, the bottom plate 100 has a ladder structure 110, a liquid storage tank 120, a diversion channel 130 and a sample loading area 140. The ladder structure 110 is distributed in an arc shape. A capillary layer is formed between the ladder structure 110 and the cover plate 200. The liquid storage tank 120 and the diversion channel 130 are located on both sides of the ladder structure 110. One end of the diversion channel 130 is connected to the ladder structure 110, and the sample loading area 140 is connected to the other end of the diversion channel 130. The diversion channel 130 and the sample loading area 140 are both There are multiple; the cover plate 200 is provided with a first guide channel 230, the first guide channel 230 is located above the guide channel 130, and the first guide channel 230 is at least partially located on the step along the projection perpendicular to the direction of the cover plate 200. on structure 110. Among them, the first flow guide channel 230 is a capillary channel, which can drive the sample to be detected to flow from the step structure 110 side to the upper sample area 140 side. The capillary layer generates capillary force on the sample to be detected, which can drive the sample to be detected to move in a directional manner. The capillary layer is formed between the step structure 110 and the cover plate 200. The capillary force of the capillary layer on the sample to be detected is limited. Therefore, the provision of the first diversion channel 230 can further drive the sample to be detected in the diversion channel 130 upward. Sample area 140 moves to one side.
实际应用中，可以通过调整导流渠130、上样区140及第一导流通道230等结构的数量来满足不同的联合检测项目。In practical applications, different joint detection items can be satisfied by adjusting the number of structures such as the diversion channel 130, the sample loading area 140, and the first diversion channel 230.
本实施例中，阶梯结构110呈圆弧状分布，使得储液槽120呈现为扇形。其中，各导流渠130的长度一致；各第一导流通道230的长度也一致。由此，能够保证储液槽120中的待检测样本到达各个上样区140的时间相同，利于实现多个检测试纸同时开始检测。In this embodiment, the ladder structure 110 is distributed in an arc shape, so that the liquid storage tank 120 appears in a fan shape. Wherein, the lengths of each diversion channel 130 are consistent; the lengths of each first diversion channel 230 are also consistent. This ensures that the samples to be detected in the liquid storage tank 120 arrive at each sample loading area 140 at the same time, which facilitates the realization of multiple detection test strips starting detection at the same time.
检测时，检测试纸的上样部放置于上样区140内，储液槽120内的待检测样本流经阶梯结构110、第一导流通道230及230导流渠130后到达上样区140，并与检测试纸的上样部接触以完成上样。During detection, the sample loading part of the test paper is placed in the sample loading area 140, and the sample to be tested in the liquid storage tank 120 flows through the ladder structure 110, the first diversion channel 230 and the 230 diversion channel 130 and then reaches the sample loading area 140. , and come into contact with the sample loading part of the test paper to complete the sample loading.
本申请的实施例中，阶梯结构110位于储液槽120的一侧，且阶梯结构110靠近储液槽120的一侧呈弧线状，使得储液槽120内的待检测样本达到各个第一导流通道230或导流渠130的距离相等，利于使储液槽120内的待检测样本到达各个检测试纸的时间保持一致，也利于储液槽120中的待检测样本均匀分配到各个上样区140中，使得各个检测试纸能够同时开始检测；待测待检测样本在储液槽120内混匀后再同一分配到各个上样区140，避免分次加样存在的样本差异、多个被测项样本差异和检测时间差异等因素造成的检测误差和结果偏移，同时也提高了用户便捷操作的体验度；阶梯结构110与盖板200之间形成有毛细层，样本量超过储液槽120容积时毛细层起毛细作用将液体平均分配至各导流渠130入口处，同时盖板200中设置有第一导流通道230，入口处的液体在第一导流通道的毛细作用下自驱动引导进入上样区。在第一导流通道230及毛细层的作用下，储液槽120中的待检测样本能够被均匀的、自驱动式地引导至上样区140，利于降低加样操作带来的误差；此外，第一导流通道230与导流渠130能够引导待检测样本流向上样区140，导流渠130的横截面积较大，能够输送较多的待检测样本，利于缩短上样时间。In the embodiment of the present application, the ladder structure 110 is located on one side of the liquid storage tank 120, and the side of the ladder structure 110 close to the liquid storage tank 120 is in an arc shape, so that the sample to be detected in the liquid storage tank 120 reaches each first level. The distance between the diversion channels 230 or the diversion channels 130 is equal, which is conducive to making the time for the samples to be detected in the liquid storage tank 120 to reach each test paper consistent, and is also conducive to evenly distributing the samples to be detected in the liquid storage tank 120 to each sample. In the area 140, each test strip can start testing at the same time; the sample to be tested is mixed in the liquid storage tank 120 and then uniformly distributed to each sample loading area 140, so as to avoid sample differences and multiple samples being added in batches. Detection errors and result deviations caused by factors such as differences in measurement items, sample differences, and detection time differences also improve the user's convenient operation experience; a capillary layer is formed between the ladder structure 110 and the cover plate 200, and the sample volume exceeds the liquid storage tank When the volume is 120, the capillary layer acts as a capillary action to evenly distribute the liquid to the entrance of each diversion channel 130. At the same time, the cover plate 200 is provided with a first diversion channel 230. The liquid at the entrance automatically flows under the capillary action of the first diversion channel. The driver guides you into the sample loading area. Under the action of the first guide channel 230 and the capillary layer, the sample to be detected in the liquid storage tank 120 can be uniformly and self-drivenly guided to the sample loading area 140, which is beneficial to reducing errors caused by the sample addition operation; in addition, The first guide channel 230 and the guide channel 130 can guide the sample to be detected to flow to the sample loading area 140. The guide channel 130 has a larger cross-sectional area and can transport more samples to be detected, which is beneficial to shortening the sample loading time.
本申请的一些实施例中，参见图2和图3所示，盖板200设置有观察窗220，以方便观察检测结果。In some embodiments of the present application, as shown in Figures 2 and 3, the cover 200 is provided with an observation window 220 to facilitate observation of the detection results.
本申请的一些实施例中，参见图5所示，底板100和盖板200围合有检测腔，底板100设置有检测试纸放置区170，以限制检测试纸在检测腔内移动。进一步地，上样区140设置有第三凸起150，第三凸起150用于支撑放置于上样区140内的检测试纸。进一步地，底板100还设置有连接柱180，该连接柱180用于与盖板200连接固定。In some embodiments of the present application, as shown in FIG. 5 , the bottom plate 100 and the cover plate 200 enclose a detection cavity, and the bottom plate 100 is provided with a detection test paper placement area 170 to limit the movement of the detection test paper in the detection cavity. Further, the sample loading area 140 is provided with a third protrusion 150 , and the third protrusion 150 is used to support the detection test paper placed in the sample loading area 140 . Furthermore, the base plate 100 is also provided with a connecting column 180 , which is used to connect and fix with the cover plate 200 .
本申请的一些实施例中，参见图4和图5所示，阶梯结构110包括第一阶梯111，第一阶梯111具有第一端面1111，第一端面1111与储液槽120底部的距离为0.3～1.0mm。具体的，第一端面1111为第一阶梯111中靠近盖板200一侧的端面。向储液槽120内添加待检测样本时，第一阶梯111能够起到缓冲作用，减少了储液槽120内的液体未经混匀就漫过阶梯结构110并进入到毛细层的情况，使待检测样本混匀后再上样，利于实现多个检测试纸同时开始检测。其中，第一端面1111与储液槽120底部的距离即第一阶梯111的高度设置为0.3～1.0mm，以保证储液槽120内的待检测样本能够有足够的体积以满足多个检测试纸同时上样检测的需求。实际应用中，第一阶梯111的高度可以根据需要设置为0.4mm、0.5mm、0.6mm、0.7mm、0.8mm等。In some embodiments of the present application, as shown in FIGS. 4 and 5 , the ladder structure 110 includes a first step 111 , the first step 111 has a first end surface 1111 , and the distance between the first end surface 1111 and the bottom of the liquid reservoir 120 is 0.3. ~1.0mm. Specifically, the first end surface 1111 is the end surface of the first step 111 close to the cover plate 200 . When adding a sample to be detected into the liquid storage tank 120, the first step 111 can act as a buffer, reducing the situation that the liquid in the liquid storage tank 120 will overflow the ladder structure 110 and enter the capillary layer without being mixed, so that The test sample is mixed before loading, which facilitates the simultaneous detection of multiple test strips. Among them, the distance between the first end surface 1111 and the bottom of the liquid storage tank 120, that is, the height of the first step 111, is set to 0.3-1.0 mm to ensure that the sample to be tested in the liquid storage tank 120 has enough volume to satisfy multiple detection test strips. Simultaneous sample loading and testing requirements. In actual applications, the height of the first step 111 can be set to 0.4mm, 0.5mm, 0.6mm, 0.7mm, 0.8mm, etc. as needed.
本申请的一些实施例中，请继续参见图4和图5所示，阶梯结构110还包括第二阶梯112，第二阶梯112具有第二端面1121，第二端面1121与第一端面1111的间距为0.3～1.0mm。具体的，第二端面1121为第二阶梯112中靠近端盖一侧的端面。第二阶梯112较第一端面1111高出一定高度，增大了储液槽120的容积。值得理解，通过改变第二阶梯112的高度，能够改变储液槽120的容积。In some embodiments of the present application, please continue to refer to Figures 4 and 5. The ladder structure 110 also includes a second step 112. The second step 112 has a second end face 1121, and the distance between the second end face 1121 and the first end face 1111 is is 0.3~1.0mm. Specifically, the second end surface 1121 is the end surface of the second step 112 close to the end cap. The second step 112 is higher than the first end surface 1111 by a certain height, thereby increasing the volume of the liquid storage tank 120 . It is worth understanding that by changing the height of the second step 112, the volume of the liquid reservoir 120 can be changed.
本申请的一些实施例中，第二端面1121与盖板200限定出毛细层，毛细层的高度为0.3-0.6mm。具体的，第二端面1121与盖板200之间的间隙形成前述毛细层，第二端面1121与盖板200之间的间距为毛细层的高度。将毛细层的高度设置为0.3～0.6mm，能够使毛细层形成有自驱动式毛细作用，以驱动储液槽120内的待检测样本从储液槽120流向上样区140。实际应用中，毛细层的高度可以根据需要进行适应性调整，具体数值可以根据需要设置为0.3mm、0.4mm、0.5mm等，在此不作限定。In some embodiments of the present application, the second end surface 1121 and the cover plate 200 define a capillary layer, and the height of the capillary layer is 0.3-0.6 mm. Specifically, the gap between the second end surface 1121 and the cover plate 200 forms the aforementioned capillary layer, and the distance between the second end surface 1121 and the cover plate 200 is the height of the capillary layer. Setting the height of the capillary layer to 0.3-0.6 mm enables the capillary layer to form a self-driven capillary action to drive the sample to be detected in the liquid storage tank 120 to flow from the liquid storage tank 120 to the sample area 140 . In actual applications, the height of the capillary layer can be adjusted adaptively as needed. The specific value can be set to 0.3mm, 0.4mm, 0.5mm, etc. as needed, and is not limited here.
本申请的一些实施例中，导流渠130的长度为3～6mm。具体的，导流渠130与第一导流通道230适配。检测工作开始时，待检测样本先通过第一导流通道230流动至上样区140，然后剩余的待检测样本在第一导流通道230的引导作用下，沿着导流渠130流动至上样区140，并与位于上样区140的检测试纸接触，以完成上样。将导流渠130的长度设置为3～6mm，利于实现多个待测项目同时开始检测。应当指出，当导流渠130的长度小于3mm时，容易出现待检测样本直接涌入导流渠130并沿着导流渠130直接进入上样区140的情况，此时，待检测样本可能未经过第一导流通道230，使得待检测样本从储液槽120流动至上样区140的时间无法控制，容易导致多个检测试纸无法同时检测；当导流渠130的长度大于6mm时，待检测样本经第一导流通道230及导流渠130到达上样区140所需的时间过长，作为示例的，一些实施例中，待检测样本经第一导流通道230及导流渠130到达上样区140所需的时间大于1秒，上样速度慢，容易出现上样偏差，也不利于缩短检测时间。In some embodiments of the present application, the length of the diversion channel 130 is 3-6 mm. Specifically, the diversion channel 130 is adapted to the first diversion channel 230 . When the detection work starts, the sample to be detected first flows to the sample loading area 140 through the first guide channel 230, and then the remaining sample to be detected flows along the guide channel 130 to the sample loading area under the guidance of the first guide channel 230. 140, and come into contact with the test paper located in the sample loading area 140 to complete the sample loading. Setting the length of the diversion channel 130 to 3 to 6 mm facilitates the simultaneous detection of multiple items to be tested. It should be noted that when the length of the diversion channel 130 is less than 3 mm, it is easy for the sample to be detected to directly pour into the diversion channel 130 and directly enter the sample loading area 140 along the diversion channel 130. At this time, the sample to be detected may not be detected. After passing through the first diversion channel 230, the time for the sample to be detected to flow from the liquid storage tank 120 to the sample loading area 140 cannot be controlled, which may easily cause multiple detection test strips to be unable to detect at the same time; when the length of the diversion channel 130 is greater than 6 mm, the time for the sample to be detected to be detected cannot be controlled. The time required for the sample to reach the sample loading area 140 through the first diversion channel 230 and the diversion channel 130 is too long. As an example, in some embodiments, the sample to be detected arrives through the first diversion channel 230 and the diversion channel 130 The time required for loading the sample area 140 is more than 1 second, the sample loading speed is slow, sample loading deviation is prone to occur, and it is not conducive to shortening the detection time.
本申请的一些实施例中，盖板200还设置有第一凸起240，第一导流通道230至少部分与第一凸起240连接，且第一凸起240沿竖直方向的投影位于上样区140内。具体的，第一凸起240与检测试纸的上样部接触，用于将第一导流通道230中的待检测样本导向检测试纸的上样部，以保证及时上样。In some embodiments of the present application, the cover 200 is further provided with a first protrusion 240 , the first guide channel 230 is at least partially connected to the first protrusion 240 , and the projection of the first protrusion 240 in the vertical direction is located above Within sample area 140. Specifically, the first protrusion 240 is in contact with the sample loading part of the detection test paper, and is used to guide the sample to be detected in the first diversion channel 230 to the sample loading part of the detection test paper to ensure timely loading of the sample.
本申请的一些实施例中，参见图9所示，第一导流通道230贯穿第一凸起240设置。由此，使得第一导流通道230与检测试纸一侧连通，利于平衡第一导流通道230两端的气压，以方便储液槽120内的待检测样本沿着第一导流通道230流向上样区140。In some embodiments of the present application, as shown in FIG. 9 , the first flow guide channel 230 is provided through the first protrusion 240 . As a result, the first flow guide channel 230 is connected to one side of the test paper, which helps balance the air pressure at both ends of the first flow guide channel 230 and facilitates the flow of the sample to be detected in the liquid storage tank 120 upward along the first flow guide channel 230. Sample area 140.
本申请的一些实施例中，请继续参见图9所示，沿垂直于盖板200方向，第一凸起240凸出于第一导流通道230底面0.2～0.5mm设置，以使经过第一导流通道230流动至上样区140的待检测样本沿着第一凸起240立即进入检测试纸的上样部。检测试纸的上样部的材质通常为玻璃纤维，该类材质蓬松、吸水性能强，若不设置第一凸起240或第一凸起240的高度小于0.2mm时，容易出现第一导流通道230末端液体不能与上样部接触的情况，进而使待检测样本不能及时上样检测。当第一凸起240的高度大于0.5mm时，第一凸起240会与检测试纸的上样部紧密挤压，会导致检测试纸上样困难进而无法完成检测。In some embodiments of the present application, please continue to refer to FIG. 9 . In the direction perpendicular to the cover plate 200 , the first protrusion 240 protrudes from the bottom surface of the first guide channel 230 by 0.2 to 0.5 mm, so that the first protrusion 240 passes through the first guide channel 230 . The sample to be detected that flows from the flow guide channel 230 to the sample loading area 140 immediately enters the sample loading part of the detection test paper along the first protrusion 240 . The material of the loading part of the test paper is usually glass fiber. This type of material is fluffy and has strong water absorption. If the first protrusion 240 is not provided or the height of the first protrusion 240 is less than 0.2 mm, the first diversion channel will easily appear. The liquid at the end of 230 cannot come into contact with the sample loading part, so that the sample to be tested cannot be loaded and tested in time. When the height of the first protrusion 240 is greater than 0.5 mm, the first protrusion 240 will be tightly squeezed with the sample loading part of the test paper, which will cause difficulty in loading the test paper and make it impossible to complete the test.
本申请的一些实施例中，底板100设置有第二导流通道160，第二导流通道160的一端与储液槽120连通，第二导流通道160的另一端延伸至阶梯结构110的端面上。具体的，第二导流通道160由储液槽120底部朝向导流渠130方向延伸，导流渠130位于第二导流通道160的延长侧，第二导流通道160能够为待检测样本流向阶梯结构110起到导向作用。第二导流通道160的设置，能够加速待检测样本从储液槽120流向阶梯结构110，进而缩短检测时间。应当指出，第二导流通道160远离储液槽120的一端距离导流渠130有一定距离，以降低待检测样本直接从阶梯结构110流向导流渠130中。In some embodiments of the present application, the bottom plate 100 is provided with a second flow guide channel 160 , one end of the second flow guide channel 160 is connected to the liquid storage tank 120 , and the other end of the second flow guide channel 160 extends to the end surface of the ladder structure 110 superior. Specifically, the second guide channel 160 extends from the bottom of the liquid storage tank 120 toward the guide channel 130. The guide channel 130 is located on the extended side of the second guide channel 160. The second guide channel 160 can provide a flow direction for the sample to be detected. The ladder structure 110 plays a guiding role. The provision of the second flow guide channel 160 can accelerate the flow of the sample to be detected from the liquid storage tank 120 to the ladder structure 110, thereby shortening the detection time. It should be noted that the end of the second guide channel 160 away from the liquid storage tank 120 is at a certain distance from the guide channel 130 to reduce the sample to be detected from flowing directly from the ladder structure 110 into the guide channel 130 .
进一步地，第二导流通道160远离储液槽120的一端与第一导流通道230靠近储液槽120的一端在垂直于盖板200的方向上至少部分重叠，以使得沿第二导流通道160流向阶梯结构110后，能够继续沿着第一导流通道230流向上样区140，从而进一步提升上样效率，缩短检测时间。Further, an end of the second flow guide channel 160 away from the liquid storage tank 120 and an end of the first flow guide channel 230 close to the liquid storage tank 120 at least partially overlap in a direction perpendicular to the cover plate 200, so that along the second flow guide channel 160, an end thereof close to the liquid storage tank 120 at least partially overlaps. After the channel 160 flows to the step structure 110, it can continue to flow to the sample loading area 140 along the first guide channel 230, thereby further improving the sample loading efficiency and shortening the detection time.
本申请的一种实施例中，参见图4至图8所示，第二导流通道160与储液槽120连通的一端低于储液槽120的底面设置，以方便储液槽120内的待检测样本流向第二导流通道160。In one embodiment of the present application, as shown in FIGS. 4 to 8 , one end of the second flow guide channel 160 communicating with the liquid storage tank 120 is set lower than the bottom surface of the liquid storage tank 120 to facilitate the flow of water in the liquid storage tank 120 . The sample to be detected flows to the second flow guide channel 160.
本申请的一些实施例中，参见图1至图4所示，盖板200具有加样孔210，加样孔210的位置与储液槽120对应，盖板200还设置有第二凸起250，第二凸起250围绕加样孔210并呈放射状设置。第二凸起250的设置，能够在储液槽120的待检测样本流向上样区140时，将导流渠130及第一导流通道230内存在的空气以气泡的形式引向加样孔210的一侧，起到排去导流渠130及第一导流通道230内的气体的作用，以避免导流渠130及第一导流通道230的空气阻碍待检测样本向上样区140一侧移动。In some embodiments of the present application, as shown in FIGS. 1 to 4 , the cover plate 200 has a sampling hole 210 , the position of the sampling hole 210 corresponds to the liquid storage tank 120 , and the cover plate 200 is also provided with a second protrusion 250 , the second protrusions 250 surround the sampling hole 210 and are arranged radially. The arrangement of the second protrusion 250 can guide the air existing in the guide channel 130 and the first guide channel 230 to the sampling hole in the form of bubbles when the sample to be detected in the liquid storage tank 120 flows into the upper sample area 140 One side of 210 plays the role of discharging the gas in the guide channel 130 and the first guide channel 230, so as to prevent the air in the guide channel 130 and the first guide channel 230 from blocking the sample to be detected from moving upward to the sample area 140. Move sideways.
本申请的一些实施例中，参见图4所示，沿靠近加样孔210方向，第二凸起250的高度逐渐减小。由此，能够方便前述气泡向靠近加样孔210一侧移动。应当理解，第二凸起250的高度指的是：在垂直于盖板200方向上，第二凸起250与盖板200端面的距离。In some embodiments of the present application, as shown in FIG. 4 , the height of the second protrusion 250 gradually decreases along the direction approaching the sampling hole 210 . This facilitates the movement of the bubbles to the side closer to the sampling hole 210 . It should be understood that the height of the second protrusion 250 refers to the distance between the second protrusion 250 and the end surface of the cover plate 200 in a direction perpendicular to the cover plate 200 .
上面结合附图对本申请实施例作了详细说明，但是本申请不限于上述实施例，在所属技术领域普通技术人员所具备的知识范围内，还可以在不脱离本申请宗旨的前提下作出各种变化。此外，在不冲突的情况下，本申请的实施例及实施例中的特征可以相互组合。The embodiments of the present application have been described in detail above in conjunction with the accompanying drawings. However, the present application is not limited to the above-mentioned embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art, various embodiments can be made without departing from the purpose of the present application. Variety. In addition, the embodiments of the present application and the features in the embodiments may be combined with each other without conflict.
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310697294.7ACN116870977A (en) | 2023-06-12 | 2023-06-12 | Microfluidic joint inspection card shell and device |
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310697294.7ACN116870977A (en) | 2023-06-12 | 2023-06-12 | Microfluidic joint inspection card shell and device |
| Publication Number | Publication Date |
|---|---|
| CN116870977Atrue CN116870977A (en) | 2023-10-13 |
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310697294.7APendingCN116870977A (en) | 2023-06-12 | 2023-06-12 | Microfluidic joint inspection card shell and device |
| Country | Link |
|---|---|
| CN (1) | CN116870977A (en) |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114798020A (en)* | 2022-03-28 | 2022-07-29 | 四川成电医联科技咨询有限公司 | Multi-channel electrochemical micro-fluidic test card and manufacturing method thereof |
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| US20020104759A1 (en)* | 2000-12-07 | 2002-08-08 | The Board Of Governors Of The University Of Alberta And Micralyne Inc. | Sample analysis on a microchip |
| US20040038426A1 (en)* | 2002-08-22 | 2004-02-26 | Scott Manalis | Measurement of concentrations and binding energetics |
| CN207488191U (en)* | 2017-08-21 | 2018-06-12 | 上海荒岛科技有限公司 | A kind of substrate and detection block |
| CN109030812A (en)* | 2018-07-19 | 2018-12-18 | 东莞东阳光科研发有限公司 | A kind of micro-fluidic chip based on immune detection and biochemistry detection, detector and detection method |
| CN113512490A (en)* | 2021-04-19 | 2021-10-19 | 杭州优思达生物技术有限公司 | Self-driven micro-fluidic detection device and application thereof |
| CN220214971U (en)* | 2023-06-12 | 2023-12-22 | 广州朗坤生物科技有限公司 | Microfluidic joint inspection card shell and device |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020104759A1 (en)* | 2000-12-07 | 2002-08-08 | The Board Of Governors Of The University Of Alberta And Micralyne Inc. | Sample analysis on a microchip |
| US20040038426A1 (en)* | 2002-08-22 | 2004-02-26 | Scott Manalis | Measurement of concentrations and binding energetics |
| CN207488191U (en)* | 2017-08-21 | 2018-06-12 | 上海荒岛科技有限公司 | A kind of substrate and detection block |
| CN109030812A (en)* | 2018-07-19 | 2018-12-18 | 东莞东阳光科研发有限公司 | A kind of micro-fluidic chip based on immune detection and biochemistry detection, detector and detection method |
| CN113512490A (en)* | 2021-04-19 | 2021-10-19 | 杭州优思达生物技术有限公司 | Self-driven micro-fluidic detection device and application thereof |
| CN220214971U (en)* | 2023-06-12 | 2023-12-22 | 广州朗坤生物科技有限公司 | Microfluidic joint inspection card shell and device |
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114798020A (en)* | 2022-03-28 | 2022-07-29 | 四川成电医联科技咨询有限公司 | Multi-channel electrochemical micro-fluidic test card and manufacturing method thereof |
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