CN116510093B - A method for preparing medical anti-adhesion material - Google Patents

Abstract

Translated fromChinese

本发明公开了一种医用防粘连材料的制备方法，防粘连智能中单正面为无纺布材料，其表面添加生物防粘连活性透明质酸材料涂层，背面为PE防油、防水、防血膜，防水透气，无刺激。制备出表面洁净的无纺布材料，将制备的不同质量的双键化透明质酸聚合物溶解到超纯水中，形成聚合物溶液，并加入0.1％‑1.0％的光引发剂苯基(2,4,6‑三甲基苯甲酰基)磷酸锂盐，形成最终的混合溶液；将处理后的无纺布材料浸入到含有引发剂的透明质酸溶液中，取出无纺布材料，置于紫外灯下交联反应1‑30分钟，随后利用超纯水清洗无纺布材料三次，最终室温干燥24小时。本发明防止中单与患者皮损粘连，避免皮损与中单分离时造成的二次损伤，提高患者皮损的恢复进程，增加舒适度。

The invention discloses a preparation method of a medical anti-adhesion material, and the front side of the anti-adhesion intelligent single is a non-woven material, and its surface adds a biological anti-adhesion active hyaluronic acid material coating, and the back side is a PE anti-oil, waterproof, and anti-blood film, which is waterproof and breathable, without stimulation. Prepare a non-woven material with clean surface, dissolve the prepared double-bonded hyaluronic acid polymer of different masses in ultrapure water, form a polymer solution, and add 0.1%-1.0% of the photoinitiator phenyl (2,4,6-trimethylbenzoyl) lithium phosphate to form a final mixed solution; The non-woven material after treatment is immersed in the hyaluronic acid solution containing an initiator, take out the non-woven material, place under ultraviolet lamp for cross-linking reaction 1-30 minutes, then use ultrapure water to clean the non-woven material three times, and finally dry at room temperature for 24 hours. The present invention prevents the single from adhering to the patient's skin lesions, avoids the secondary damage caused when the skin lesions are separated from the single, improves the recovery process of the patient's skin lesions, and increases comfort.

Description

Preparation method of medical anti-adhesion material

Technical Field

The invention belongs to a preparation method of an anti-adhesion material, and particularly relates to a preparation method of a medical anti-adhesion middle single material. Is suitable for patients with erosive and exudative skin diseases, can effectively prevent skin injury and bedsheet adhesion, and is beneficial to healing of skin injury of patients.

Background

The hospitalization department of dermatology receives a great number of patients with damaged skin barrier and erosion and exudation, including bullous dermatoses, drug eruptions, herpes zoster and other patients. During treatment, most of the skin wound surfaces of patients are in an exposed state, and in order to protect the wound surfaces and avoid the pollution of the bedding and clothing by seepage, a middle sheet is usually required to be paved on a bed sheet. At present, two types of medical disposable medium sheets are commonly used in hospitals, one type of medical disposable medium sheet consists of non-woven fabrics and plastic films, one type of medical disposable medium sheet consists of plastic films, non-woven fabrics, fluff pulp, high polymers and other materials, and the common defects of the two types of medium sheets have no anti-adhesion effect, so that skin damage of pressed parts is adhered with the medium sheets together, and secondary damage of skin can be caused by tearing during separation, so that skin damage healing is seriously affected. In addition, in order to avoid skin injury and adhesion of the traditional Chinese medicine, some patients cannot turn over for a long time, so that wound surfaces of pressed parts are difficult to heal, and some patients adopt passive positions such as sitting positions, so that sleeping rest is influenced, physical consumption is caused, and disease recovery is influenced. The novel anti-adhesion medium sheet is added with the biological anti-adhesion active hyaluronic acid material coating on the surface, so that the adhesion between a wound surface and the medium sheet can be avoided, and a series of problems caused by the adhesion between the medium sheet and the skin are overcome.

Disclosure of Invention

The invention aims to overcome the defects in the use process of the traditional medium sheet, and by improving the material composition and the surface structure of the original medium sheet, the medium sheet is prevented from being adhered to the skin damage of a patient, the secondary damage caused by the separation of the skin damage and the medium sheet is avoided, the rest sleep of the patient is facilitated, the recovery process of the skin damage of the patient is improved, and the comfort level is increased. Is convenient for the patients with wound surfaces to use and is popularized in a large range in China.

The front surface of the anti-adhesion intelligent medium sheet is made of non-woven fabric material, a biological anti-adhesion active hyaluronic acid material coating is added on the surface of the medium sheet, the back is PE oil-proof, waterproof and blood-proof film, waterproof and breathable, and has no stimulation.

The technical scheme of the invention is realized by the following specific preparation steps:

Firstly, sequentially ultrasonically cleaning a medical non-woven fabric material for 30 minutes by using solvents such as water, ethanol and acetone to prepare the non-woven fabric material with clean surface, wherein the mass ratio of the medical non-woven fabric material to the solvent is (5-100);

Preparing an aqueous solution of hyaluronic acid with the mass volume fraction of 0.1% -2%, dripping N, N-dimethylformamide DMF and water with the volume ratio of 1:3-2:3 into the aqueous solution of hyaluronic acid, uniformly stirring, cooling to 4 ℃, adding methacrylic anhydride and hyaluronic acid with the molar ratio of 1:1-3:1, adjusting the pH value to 8.0-8.5,4 ℃ by using sodium hydroxide (0.5M), reacting for 12-20 hours, centrifuging, and freeze-drying for 12 hours to obtain double-bonded hyaluronic acid;

dissolving the prepared double-bonded hyaluronic acid polymers with different mass into ultrapure water to form polymer solutions with different mass volume concentrations (0.1% -10%), and adding 0.1% -1.0% of photo-initiator phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate to form a final mixed solution;

Immersing the treated non-woven fabric material into the hyaluronic acid solution containing the initiator, vacuumizing for 30 minutes, maintaining the vacuum degree at 1Pa, taking out the non-woven fabric material, performing crosslinking reaction for 1-30 minutes under a 365nm ultraviolet lamp, then cleaning the non-woven fabric material with ultrapure water for three times, and finally drying at room temperature for 24 hours.

The invention has the following remarkable effects

1. The medical close-fitting anti-adhesion intelligent middle sheet is used, the wound surface cannot be adhered with the middle sheet, secondary damage caused by adhesion between the middle sheet and the wound surface is avoided, and the time for healing skin damage is shortened.

2. The anti-sticking intelligent middle bill meets the requirement of a patient on the comfort of the middle bill, can rest and sleep safely, and is beneficial to disease recovery.

3. The anti-adhesion intelligent medium sheet can be cut and used at will and used as an anti-adhesion pad, for example, the anti-adhesion intelligent medium sheet is arranged between a cuff and a skin lesion when blood pressure is measured, and a sphygmomanometer cuff is polluted by seepage.

4. Can adsorb small amount of exudation, has adjuvant therapeutic effect, and can promote wound healing.

5. The intelligent medium single material for preventing adhesion in the future can be used for manufacturing medical anti-adhesion gauze, and is suitable for wound nursing and binding after surgical operation.

6. Can be used as anti-adhesion clothes for patients with skin erosion.

7. The use cost is low (yuan/personal), and the clinical popularization is facilitated.

8. Is convenient for wide popularization of domestic dermatology, burn department and surgery.

Drawings

FIG. 1 illustrates a single junction pattern in anti-blocking intelligence;

1. nonwoven 2. Coating 3.PE

Detailed Description

The invention comprises the following specific steps:

1) Firstly, ultrasonically cleaning a medical non-woven fabric material for 30 minutes by using water, ultrasonically cleaning by using ethanol for 30 minutes, ultrasonically cleaning by using acetone for 30 minutes, freeze-drying the treated non-woven fabric material for 12 hours to obtain a pretreated non-woven fabric material, and storing the pretreated non-woven fabric material in a drying environment for further use;

2) 2g of hyaluronic acid powder with certain mass is dissolved in 100mL of ultrapure water to prepare aqueous solution of hyaluronic acid with mass volume fraction of 2%, then N, N-Dimethylformamide (DMF) (with water volume ratio of 1:3 to 2:3) is added into the aqueous solution of hyaluronic acid dropwise, the mixture is stirred uniformly and cooled to 4 ℃, methacrylic anhydride (with the molar ratio of 1:1 to 3:1) is added, sodium hydroxide (0.5M) is used for adjusting the PH value to 8.0-8.5,4 ℃ for reaction for 20 hours, and double-bonded hyaluronic acid is obtained after centrifugal separation and freeze drying for 12 hours;

3) Dissolving the prepared double-bonded hyaluronic acid polymers with different mass into ultrapure water to form polymer solutions with different mass volume concentrations (0.1% -10%), and adding 0.1% -1.0% of photo-initiator phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate to form a final mixed solution;

4) Immersing the treated non-woven fabric material into the hyaluronic acid solution containing the initiator, vacuumizing for 30 minutes, maintaining the vacuum degree at 1Pa, taking out the non-woven fabric material, performing crosslinking reaction for 1-30 minutes under a 365nm ultraviolet lamp, then cleaning the non-woven fabric material with ultrapure water for three times, and finally drying at room temperature for 24 hours;

Example 1:

Step 1), ultrasonically cleaning a medical non-woven fabric material for 30 minutes by using water, ultrasonically cleaning the medical non-woven fabric material for 30 minutes by using ethanol, ultrasonically cleaning the medical non-woven fabric material by using acetone for 30 minutes, and freeze-drying the treated non-woven fabric material for 12 hours for later use;

Step 2) preparing an aqueous solution of hyaluronic acid with a mass volume fraction of 2%, then dropwise adding N, N-Dimethylformamide (DMF) (with a water volume ratio of 2:3) into the aqueous solution of hyaluronic acid, uniformly stirring, cooling to 4 ℃, then adding methacrylic anhydride (with a hyaluronic acid molar ratio of 3:1), regulating a pH value to 8.5,4 ℃ by using sodium hydroxide (0.5M), reacting for 20 hours, centrifuging, and freeze-drying for 12 hours to obtain double-bonded hyaluronic acid;

Step 3) dissolving double-bonded hyaluronic acid polymer into ultrapure water to prepare a polymer solution with the mass volume concentration of 2%, and adding 0.5% of photoinitiator phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate to form a final mixed solution;

And 4) immersing the treated non-woven fabric material into the hyaluronic acid solution containing the initiator, vacuumizing for 30 minutes, maintaining the vacuum degree at 1Pa, taking out the non-woven fabric material, performing crosslinking reaction for 15 minutes under a 365nm ultraviolet lamp, then washing the non-woven fabric material with ultrapure water for three times, and finally drying at room temperature for 24 hours to obtain the anti-adhesion non-woven fabric material, wherein the prepared material can also be used for disposable anti-adhesion clothes, anti-adhesion isolation pads and anti-adhesion gauze.

Example 2:

Step 1), ultrasonically cleaning a medical non-woven fabric material for 30 minutes by using water, ultrasonically cleaning the medical non-woven fabric material for 30 minutes by using ethanol, ultrasonically cleaning the medical non-woven fabric material by using acetone for 30 minutes, and freeze-drying the treated non-woven fabric material for 12 hours for later use;

Step 2) preparing an aqueous solution of hyaluronic acid with a mass volume fraction of 2%, then dropwise adding N, N-Dimethylformamide (DMF) (with a water volume ratio of 2:3) into the aqueous solution of hyaluronic acid, uniformly stirring, cooling to 4 ℃, then adding methacrylic anhydride (with a hyaluronic acid molar ratio of 3:1), regulating a pH value to 8.5,4 ℃ by using sodium hydroxide (0.5M), reacting for 20 hours, centrifuging, and freeze-drying for 12 hours to obtain double-bonded hyaluronic acid;

step 3) dissolving double-bonded hyaluronic acid polymer into ultrapure water to prepare polymer solution with the mass volume concentration of 4%, and adding 0.6% of photoinitiator phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate to form a final mixed solution;

And 4) immersing the treated non-woven fabric material into the hyaluronic acid solution containing the initiator, vacuumizing for 30 minutes, maintaining the vacuum degree at 1Pa, taking out the non-woven fabric material, performing crosslinking reaction for 15 minutes under a 365nm ultraviolet lamp, then washing the non-woven fabric material with ultrapure water for three times, and finally drying at room temperature for 24 hours to obtain the anti-adhesion non-woven fabric material, wherein the prepared material can also be used for disposable anti-adhesion clothes, anti-adhesion isolation pads and anti-adhesion gauze.

Example 3:

Step 1), ultrasonically cleaning a medical non-woven fabric material for 30 minutes by using water, ultrasonically cleaning the medical non-woven fabric material for 30 minutes by using ethanol, ultrasonically cleaning the medical non-woven fabric material by using acetone for 30 minutes, and freeze-drying the treated non-woven fabric material for 12 hours for later use;

Step 2) preparing an aqueous solution of hyaluronic acid with a mass volume fraction of 2%, then dropwise adding N, N-Dimethylformamide (DMF) (with a water volume ratio of 2:3) into the aqueous solution of hyaluronic acid, uniformly stirring, cooling to 4 ℃, then adding methacrylic anhydride (with a hyaluronic acid molar ratio of 3:1), regulating a pH value to 8.5,4 ℃ by using sodium hydroxide (0.5M), reacting for 20 hours, centrifuging, and freeze-drying for 12 hours to obtain double-bonded hyaluronic acid;

step 3) dissolving double-bonded hyaluronic acid polymer into ultrapure water to prepare a polymer solution with the mass volume concentration of 6%, and adding 0.8% of photo-initiator phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate to form a final mixed solution;

And 4) immersing the treated non-woven fabric material into the hyaluronic acid solution containing the initiator, vacuumizing for 30 minutes, maintaining the vacuum degree at 1Pa, taking out the non-woven fabric material, performing crosslinking reaction for 15 minutes under a 365nm ultraviolet lamp, then washing the non-woven fabric material with ultrapure water for three times, and finally drying at room temperature for 24 hours to obtain the anti-adhesion non-woven fabric material, wherein the prepared material can also be used for disposable anti-adhesion clothes, anti-adhesion isolation pads and anti-adhesion gauze.

Claims (9)

Translated fromChinese

1.一种医用防粘连材料的制备方法，其特征在于：1. A method for preparing a medical anti-adhesion material, characterized in that:首先将医用无纺布材料利用水、乙醇、丙酮溶剂依次超声清洗10-30分钟，制备出表面洁净的无纺布材料；医用无纺布材料与溶剂的比例为质量比1：(5-100)；First, the medical non-woven fabric material is ultrasonically cleaned in sequence with water, ethanol, and acetone solvents for 10-30 minutes to prepare a non-woven fabric material with a clean surface; the ratio of the medical non-woven fabric material to the solvent is 1:(5-100) by mass;配置质量体积分数为0.1％-2％的透明质酸水溶液，然后将与水体积比为1:3到2:3的N,N-二甲基甲酰胺DMF溶剂滴加到透明质酸溶液中，搅拌均匀，冷却到0-4摄氏度，然后加入甲基丙烯酸酐，利用氢氧化钠调节溶液pH值为8.0-8.5，在4摄氏度下反应，离心分离后冷冻干燥得到双键化透明质酸；A hyaluronic acid aqueous solution with a mass volume fraction of 0.1%-2% is prepared, and then N,N-dimethylformamide DMF solvent with a volume ratio of 1:3 to 2:3 with water is added dropwise to the hyaluronic acid solution, stirred evenly, cooled to 0-4 degrees Celsius, and then methacrylic anhydride is added, and the pH value of the solution is adjusted to 8.0-8.5 with sodium hydroxide, reacted at 4 degrees Celsius, centrifuged and freeze-dried to obtain double-bonded hyaluronic acid;将制备的双键化透明质酸溶解到超纯水中，形成质量体积浓度为0.1％-10％的双键化透明质酸溶液，并加入光引发剂苯基(2,4,6-三甲基苯甲酰基)磷酸锂盐，形成最终的混合溶液；Dissolving the prepared double-bonded hyaluronic acid in ultrapure water to form a double-bonded hyaluronic acid solution with a mass volume concentration of 0.1%-10%, and adding a photoinitiator phenyl (2,4,6-trimethylbenzoyl) lithium phosphate to form a final mixed solution;将溶剂处理后的无纺布材料浸入到上述的含有光引发剂苯基(2,4,6-三甲基苯甲酰基)磷酸锂盐的透明质酸溶液中，抽真空30-50分钟，取出无纺布材料，置于紫外灯下交联反应，随后利用超纯水清洗无纺布材料三次，最终室温干燥，即得医用防粘连材料。The solvent-treated non-woven fabric material is immersed in the above-mentioned hyaluronic acid solution containing the photoinitiator phenyl (2,4,6-trimethylbenzoyl) lithium phosphate, and vacuumed for 30-50 minutes. The non-woven fabric material is taken out and placed under ultraviolet light for cross-linking reaction. The non-woven fabric material is then washed three times with ultrapure water and finally dried at room temperature to obtain a medical anti-adhesion material.2.根据权利要求1所述的医用防粘连材料的制备方法，其特征在于，医用无纺布材料利用水、乙醇、丙酮溶剂依次超声清洗10-30分钟。2. The method for preparing the medical anti-adhesion material according to claim 1, characterized in that the medical non-woven fabric material is ultrasonically cleaned in sequence using water, ethanol and acetone solvents for 10-30 minutes.3.根据权利要求1所述的医用防粘连材料的制备方法，其特征在于，N,N-二甲基甲酰胺DMF与水体积比为1:3-2:3。3. The preparation method of medical anti-adhesion material according to claim 1, characterized in that the volume ratio of N,N-dimethylformamide DMF to water is 1:3-2:3.4.根据权利要求1所述的医用防粘连材料的制备方法，其特征在于，甲基丙烯酸酐与透明质酸摩尔比为1:1-3:1。4. The method for preparing the medical anti-adhesion material according to claim 1, wherein the molar ratio of methacrylic anhydride to hyaluronic acid is 1:1-3:1.5.根据权利要求1所述的医用防粘连材料的制备方法，其特征在于，氢氧化钠调节溶液pH值为8.0-8.5；4摄氏度反应12-20小时，离心分离后冷冻干燥12小时。5. The method for preparing the medical anti-adhesion material according to claim 1, characterized in that the pH value of the solution is adjusted to 8.0-8.5 by sodium hydroxide; the solution is reacted at 4 degrees Celsius for 12-20 hours, and the solution is freeze-dried for 12 hours after centrifugation.6.根据权利要求1所述的医用防粘连材料的制备方法，其特征在于，双键化透明质酸溶液的质量体积浓度为0.1％-10％。6 . The method for preparing the medical anti-adhesion material according to claim 1 , wherein the mass volume concentration of the double-bonded hyaluronic acid solution is 0.1%-10%.7.根据权利要求1所述的医用防粘连材料的制备方法，其特征在于，光引发剂苯基(2,4,6-三甲基苯甲酰基)磷酸锂盐的质量体积浓度为0.1％-1.0％。7. The method for preparing the medical anti-adhesion material according to claim 1, characterized in that the mass volume concentration of the photoinitiator phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt is 0.1%-1.0%.8.根据权利要求1所述的医用防粘连材料的制备方法，其特征在于，溶剂处理后的无纺布材料浸入到上述的含有光引发剂苯基(2,4,6-三甲基苯甲酰基)磷酸锂盐的透明质酸溶液中，抽真空时间为30-50分钟，真空度维持在1Pa。8. The method for preparing a medical anti-adhesion material according to claim 1, characterized in that the solvent-treated non-woven fabric material is immersed in the hyaluronic acid solution containing the photoinitiator phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt, the vacuum time is 30-50 minutes, and the vacuum degree is maintained at 1 Pa.9.根据权利要求1所述的医用防粘连材料的制备方法，其特征在于，无纺布材料置于365nm紫外灯下交联反应1-30分钟，室温干燥24小时。9. The method for preparing the medical anti-adhesion material according to claim 1, characterized in that the non-woven fabric material is placed under a 365nm ultraviolet lamp for a cross-linking reaction for 1-30 minutes and dried at room temperature for 24 hours.