CN116243001A

Movatterモバイル変換

Info

Publication number: CN116243001A
Application number: CN202310217115.5A
Authority: CN
Inventors: 王萍; 邓顺宣; 贺青松
Original assignee: Chengkou County Agricultural Comprehensive Testing Center
Current assignee: Chengkou County Agricultural Comprehensive Testing Center
Priority date: 2023-03-07
Filing date: 2023-03-07
Publication date: 2023-06-09

Abstract

Translated fromChinese

本发明公开了一种活体动物疫病抗原抗体快速检测仪，涉及动物疫病技术领域，其技术方案要点是：包括检测试剂盒、采样棉签和样本处理试管，所述检测试剂盒包括底板、盖板、镜片和试剂条，所述底板与盖板固定连接，所述底板上设有固定区，所述固定区下端连接有传递区，所述固定区内置有试剂条，所述试剂条尾部与传递区相连，所述盖板上设有结果显示区，所述结果显示区表面覆盖镜片，所述镜片与盖板焊接，所述结果显示区中心处设有显示槽，所述盖板靠近下边缘处设有加样区，所述加样区与传递区连通。具有采样简单易操作，样品处理方式便捷，且能够判断活体动物是否感染某种疫病抗原或存在某种疫病抗体的效果。

The invention discloses a rapid detection instrument for live animal epidemic antigens and antibodies, which relates to the technical field of animal epidemics. The key points of the technical solution are: a detection kit, a sampling cotton swab and a sample processing test tube, the detection kit includes a bottom plate, a cover plate, The lens and the reagent strip, the bottom plate is fixedly connected with the cover plate, the bottom plate is provided with a fixed area, the lower end of the fixed area is connected with a transfer area, the fixed area is built with a reagent strip, and the tail of the reagent strip is connected to the transfer area Connected, the cover is provided with a result display area, the surface of the result display area is covered with lenses, the lens is welded to the cover plate, a display groove is provided at the center of the result display area, and the cover plate is close to the lower edge A sample application area is provided, and the sample application area communicates with the transfer area. It has the advantages of simple and easy sampling, convenient sample processing, and the ability to determine whether a living animal is infected with a certain disease antigen or has a certain disease antibody.

Description

Translated fromChinese

一种活体动物疫病抗原抗体快速检测仪A rapid detection instrument for live animal epidemic antigen and antibody

技术领域technical field

本发明涉及动物疫病技术领域，更具体地说，它涉及一种活体动物疫病抗原抗体快速检测仪。The invention relates to the technical field of animal epidemics, more specifically, it relates to a rapid detection instrument for antigens and antibodies of living animal epidemics.

背景技术Background technique

动物疫病，是指动物传染病、寄生虫病。对人与动物危害严重，需要采取紧急、严厉的强制预防、控制、扑灭等措施的为一类疫病。可能造成重大经济损失，需要采取严格控制、扑灭等措施，防止扩散的为二类疫病。常见多发、可能造成重大经济损失，需要控制和净化的为三类疫病。Animal epidemics refer to animal infectious diseases and parasitic diseases. Class I epidemics are serious hazards to humans and animals that require urgent and severe mandatory prevention, control, and extermination measures. It may cause heavy economic losses, and measures such as strict control and extermination are required to prevent the spread of the second-class disease. It is common and frequently occurs, and may cause major economic losses. The three types of diseases need to be controlled and purified.

目前现有技术中已经出现很多对动物疫病进行分析检测的产品，但是其普遍存在采样困难，样本处理的操作步骤繁杂等问题，不适用于我国目前的国情。现在亟需一种操作方法简单的活体动物疫病抗原抗体快速检测仪。At present, many products for the analysis and detection of animal diseases have appeared in the prior art, but they generally have problems such as difficult sampling and complicated operation steps for sample processing, which are not suitable for my country's current national conditions. There is an urgent need for a rapid detection instrument for live animal disease antigen and antibody with simple operation method.

发明内容Contents of the invention

本发明的目的是提供一种活体动物疫病抗原抗体快速检测仪，采样简单易操作，样品处理方式便捷，且能够判断活体动物是否感染某种疫病的抗原或存在某种疫病的抗体。The purpose of the present invention is to provide a rapid detection instrument for live animal disease antigen and antibody, which is simple and easy to operate, sample processing is convenient, and can determine whether a living animal is infected with a certain disease antigen or has a certain disease antibody.

本发明的上述技术目的是通过以下技术方案得以实现的：一种活体动物疫病抗原抗体快速检测仪，包括检测试剂盒、采样棉签和样本处理试管，所述检测试剂盒包括底板、盖板、镜片和试剂条，所述底板与盖板固定连接，所述底板上设有固定区，所述固定区下端连接有传递区，所述固定区内置有试剂条，所述试剂条尾部与传递区相连，所述盖板上设有结果显示区，所述结果显示区表面覆盖镜片，所述镜片与盖板焊接，所述结果显示区中心处设有显示槽，所述盖板靠近下边缘处设有加样区，所述加样区与传递区连通。The above-mentioned technical purpose of the present invention is achieved by the following technical scheme: a kind of live animal epidemic disease antigen antibody rapid detection instrument, comprises detection kit, sampling cotton swab and sample processing test tube, and described detection kit comprises bottom plate, cover plate, lens and a reagent strip, the base plate is fixedly connected to the cover plate, the base plate is provided with a fixed area, the lower end of the fixed area is connected to a transfer area, a reagent strip is built in the fixed area, and the tail of the reagent strip is connected to the transfer area , the cover plate is provided with a result display area, the surface of the result display area covers the lens, the lens is welded to the cover plate, a display groove is provided at the center of the result display area, and a display groove is provided near the lower edge of the cover plate. There is a sample application area which communicates with the transfer area.

通过采用上述技术方案，设置检测试剂盒用于检测抗原和抗体，设置采样棉签用于采集样本，设置样本处理试管用于给样本进行前处理，底板与盖板固定连接在一起，组合成试剂盒的大体框架，试剂条与抗原和抗体发生显色反应，用于检测抗原和抗体，固定区用于容纳试剂条，传递区用于暂时储存和传送样本液体，镜片的设置使得结果显示区与外界分离不易污染，同时又能够清楚显示结果，显示槽便于具体观察试剂条的情况，加样区的设置使得样本从样本处理试管转移至检测试剂盒变得更为容易。本装置的采样方式简单易操作，样品处理方式便捷，且能够判断活体动物是否感染某种疫病的抗原或存在某种疫病的抗体。By adopting the above technical scheme, a detection kit is set for detecting antigens and antibodies, a sampling cotton swab is set for collecting samples, a sample processing test tube is set for pre-processing the samples, the base plate and the cover plate are fixedly connected together to form a kit The general framework of the reagent strips reacts with antigens and antibodies for the detection of antigens and antibodies, the fixed area is used to accommodate the reagent strips, the transfer area is used to temporarily store and transfer sample liquids, and the setting of the lens makes the result display area and the outside world The separation is not easy to be polluted, and at the same time, the results can be clearly displayed. The display groove is convenient for observing the condition of the reagent strip. The setting of the sample loading area makes it easier for the sample to be transferred from the sample processing tube to the detection kit. The sampling method of the device is simple and easy to operate, the sample processing method is convenient, and it can determine whether a living animal is infected with a certain disease antigen or has a certain disease antibody.

本发明进一步设置为：所述采样棉签包括棉棒和棉球，所述棉棒底部设有易折区，所述易折区底部设有棉球。The present invention is further configured as follows: the sampling cotton swab includes a cotton swab and a cotton ball, the bottom of the cotton swab is provided with an easy-folding area, and the bottom of the easy-folding area is provided with a cotton ball.

通过采用上述技术方案，所述棉棒便于手持操作采样，所述棉球用于采取样本，易折区在采样结束后能够轻松折断，便于对样本进行处理。By adopting the above technical solution, the cotton swab is convenient for hand-held sampling, and the cotton ball is used for taking samples, and the frangible area can be easily broken after sampling, which is convenient for processing the samples.

本发明进一步设置为：所述样本处理试管包括试剂管、汇流区和橡胶头，所述试剂管底部固定连接汇流区，所述试剂管与汇流区之间设有压力阀，所述汇流区底部设有塑料球，所述橡胶头底部设有连接处，所述连接处与试剂管螺纹连接，所述试剂管内置有反应液。The present invention is further configured as follows: the sample processing test tube includes a reagent tube, a confluence area and a rubber head, the bottom of the reagent tube is fixedly connected to the confluence area, a pressure valve is provided between the reagent tube and the confluence area, and the bottom of the confluence area A plastic ball is provided, and a joint is provided at the bottom of the rubber head, and the joint is threaded with a reagent tube, and the reagent tube contains a reaction solution.

通过采用上述技术方案，试剂管中储存有反应液，能够将样本从采样棉球中洗脱下来，将带有样本的反应液从试剂管中转移至检测试剂盒时，汇流区使得其流速、流量都能够控制在一定范围内，避免飞溅导致污染，压力阀的设计使得在一般情况下，反应液无法自然下落，只有施加一定的压力才能顺利流出，塑料球在按压下能够自然脱落，橡胶头用于给试剂管内部施加压力。By adopting the above technical scheme, the reaction solution is stored in the reagent tube, and the sample can be eluted from the sampling cotton ball. When the reaction solution with the sample is transferred from the reagent tube to the detection kit, the confluence area makes its flow rate The flow rate can be controlled within a certain range to avoid contamination caused by splashing. The design of the pressure valve makes it impossible for the reaction liquid to fall naturally under normal circumstances. Only by applying a certain pressure can it flow out smoothly. The plastic ball can fall off naturally under pressure. Used to apply pressure to the inside of the reagent tube.

本发明进一步设置为：所述试剂条包括抗原检测试剂条和抗体检测试剂条，所述抗原检测试剂条和抗体检测试剂条采用荧光标记显色。The present invention is further configured as follows: the reagent strips include antigen detection reagent strips and antibody detection reagent strips, and the antigen detection reagent strips and antibody detection reagent strips are colored by fluorescent markers.

通过采用上述技术方案，通过荧光显色的方式使得结果简单明了，能够轻松判断。By adopting the above-mentioned technical solution, the result is simple and clear by means of fluorescent color development, and can be judged easily.

本发明进一步设置为：所述反应液中包含生理盐水、非离子型表面活性剂和防腐剂，所述非离子型表面活性剂可采用TritonX-100、月桂醇聚氧乙烯醚、伯醇聚氧乙烯醚、仲醇聚氧乙烯醚、格尔伯特醇聚氧乙烯醚、辛醇聚氧乙烯醚、异辛醇聚氧乙烯醚；所述防腐剂可采用ProClin300或者叠氮化钠。其中生理盐水含量在99.5％-99.8％，非离子型表面活性剂以及防腐剂含量均在千分之一。The present invention is further set as follows: the reaction liquid contains physiological saline, non-ionic surfactant and preservative, and the non-ionic surfactant can be TritonX-100, lauryl polyoxyethylene ether, primary alcohol polyoxyethylene Vinyl ether, secondary alcohol polyoxyethylene ether, Guerbet alcohol polyoxyethylene ether, octanol polyoxyethylene ether, isooctyl alcohol polyoxyethylene ether; the preservative can use ProClin300 or sodium azide. The content of physiological saline is 99.5%-99.8%, and the content of non-ionic surfactant and preservative is 1/1000.

通过采用上述技术方案，非离子型表面活性剂被用来做细胞破膜剂或者细胞通透剂，即在细胞膜上打孔，可以溶解细胞膜上的脂质，进而释放出细胞内的抗体或者抗原，生理盐水可以保持细胞的生物活性。By adopting the above technical scheme, non-ionic surfactants are used as cell membrane breaking agents or cell penetrating agents, that is, to punch holes in the cell membrane, which can dissolve lipids on the cell membrane, and then release intracellular antibodies or antigens , saline can maintain the biological activity of cells.

本发明进一步设置为：所述反应液pH值在6.5-7.5之间，通过磷酸二氢钾和磷酸氢二钠配置的磷酸缓冲液调节pH值，也可使用PBS缓冲液调节pH。The present invention is further set as: the pH value of the reaction solution is between 6.5-7.5, and the pH value is adjusted through a phosphate buffer prepared by potassium dihydrogen phosphate and disodium hydrogen phosphate, or a PBS buffer solution can be used to adjust the pH.

通过采用上述技术方案，建立一个缓冲体系，维持动物疫病检测标志物的酸碱度需求，同时也可调节样本的pH值。By adopting the above-mentioned technical scheme, a buffer system is established to maintain the pH requirement of the animal disease detection marker, and at the same time, the pH value of the sample can also be adjusted.

本发明进一步设置为：所述棉球由亲水性纤维和疏水性材料制成，所述亲水性纤维占比为30％-70％,所述亲水性纤维采用蚕丝、兔毛、驼毛、棉、麻、粘胶纤维，所述疏水性材料可选择涤纶、锦纶、氨纶、丙纶、氯纶、尼龙。The present invention is further set as: the cotton ball is made of hydrophilic fiber and hydrophobic material, the hydrophilic fiber accounts for 30%-70%, and the hydrophilic fiber is made of silk, rabbit hair, camel Wool, cotton, hemp, viscose fiber, polyester, nylon, spandex, polypropylene fiber, polyvinyl chloride, nylon can be selected as the hydrophobic material.

通过采用上述技术方案，亲水性纤维与疏水性材料形成毛细微孔基质结构，亲水性纤维可以吸收、输送液体，疏水性材料可自行分离液体中的目标检测物。By adopting the above technical scheme, the hydrophilic fiber and the hydrophobic material form a capillary microporous matrix structure, the hydrophilic fiber can absorb and transport liquid, and the hydrophobic material can separate target detection substances in the liquid by itself.

综上所述，本发明具有以下有益效果：In summary, the present invention has the following beneficial effects:

1、设置检测试剂盒用于检测抗原和抗体，设置采样棉签用于采集样本，设置样本处理试管用于给样本进行前处理，底板与盖板固定连接在一起，组合成试剂盒的大体框架；1. Set up detection kits for detecting antigens and antibodies, set up sampling cotton swabs for collecting samples, set up sample processing test tubes for pretreatment of samples, and fix the bottom plate and cover plate together to form the general frame of the kit;

2、试剂条与抗原和抗体发生显色反应，用于检测抗原和抗体，固定区用于容纳试剂条，传递区用于暂时储存和传送样本液体，镜片的设置使得结果显示区与外界分离不易污染，同时又能够清楚显示结果，显示槽便于具体观察试剂条的情况，加样区的设置使得样本从样本处理试管转移至检测试剂盒变得更为容易；2. Reagent strips react with antigens and antibodies to detect antigens and antibodies. The fixed area is used to accommodate reagent strips. The transfer area is used to temporarily store and transfer sample liquid. The setting of the lens makes it difficult to separate the result display area from the outside world. Contamination, and at the same time can clearly display the results, the display groove is convenient for specific observation of the condition of the reagent strip, and the setting of the sample loading area makes it easier to transfer the sample from the sample processing tube to the detection kit;

3、所述棉棒便于手持操作采样，所述棉球用于采取样本，易折区在采样结束后能够轻松折断，便于对样本进行处理，棉球中的亲水性纤维与疏水性材料形成毛细微孔基质结构，亲水性纤维可以吸收、输送液体，疏水性材料可自行分离液体中的目标检测物；3. The cotton swab is convenient for hand-held operation and sampling. The cotton ball is used to take samples. The frangible area can be easily broken after sampling, which is convenient for sample processing. The hydrophilic fiber and hydrophobic material in the cotton ball form a Capillary microporous matrix structure, hydrophilic fibers can absorb and transport liquids, and hydrophobic materials can separate target detection substances in liquids by themselves;

4、试剂管中储存有反应液，能够将样本从采样棉球中洗脱下来，将带有样本的反应液从试剂管中转移至检测试剂盒时，汇流区使得其流速、流量都能够控制在一定范围内，避免飞溅导致污染，压力阀的设计使得在一般情况下，反应液无法自然下落，只有施加一定的压力才能顺利流出，塑料球在按压下能够自然脱落，橡胶头用于给试剂管内部施加压力；反应液中的非离子型表面活性剂被用来做细胞破膜剂或者细胞通透剂，即在细胞膜上打孔，可以溶解细胞膜上的脂质，进而释放出细胞内的抗体或者抗原，生理盐水可以保持细胞的生物活性；4. The reaction solution is stored in the reagent tube, which can elute the sample from the sampling cotton ball. When the reaction solution with the sample is transferred from the reagent tube to the detection kit, the flow rate and flow rate of the confluence area can be controlled. Within a certain range, avoid splashing and causing pollution. The design of the pressure valve makes it impossible for the reaction liquid to fall naturally under normal circumstances. Only by applying a certain pressure can it flow out smoothly. The plastic ball can fall off naturally under pressure. The pressure is applied inside the tube; the non-ionic surfactant in the reaction solution is used as a cell membrane breaking agent or a cell penetrating agent, that is, to punch holes in the cell membrane, which can dissolve the lipids on the cell membrane, and then release the lipids in the cell. Antibodies or antigens, saline can maintain the biological activity of cells;

5、本装置的采样方式简单易操作，样品处理方式便捷，且能够判断活体动物是否感染某种疫病的抗原或存在某种疫病的抗体。5. The sampling method of this device is simple and easy to operate, the sample processing method is convenient, and it can determine whether a living animal is infected with a certain disease antigen or has a certain disease antibody.

附图说明Description of drawings

图1是本发明检测试剂盒结构示意图；Fig. 1 is the structural representation of detection kit of the present invention;

图2是本发明样本处理试管结构示意图；Fig. 2 is a schematic structural diagram of a sample processing test tube of the present invention;

图3是本发明采样棉签结构示意图；Fig. 3 is the structural representation of sampling cotton swab of the present invention;

图4是本发明检测试剂盒拆解后的结构示意图。Fig. 4 is a schematic diagram of the disassembled structure of the detection kit of the present invention.

图中：1、底板；2、盖板；3、镜片；4、显示槽；5、加样区；6、固定区；7、传递区；8、结果显示区；9、试剂条；10、试剂管；11、汇流区；12、塑料球；13、压力阀；14、橡胶头；15、反应液；16、连接处；17、棉棒；18、易折区；19、棉球。In the figure: 1. Bottom plate; 2. Cover plate; 3. Lens; 4. Display tank; 5. Sample adding area; 6. Fixing area; 7. Transfer area; 8. Results display area; 9. Reagent strip; 10. Reagent tube; 11, confluence area; 12, plastic ball; 13, pressure valve; 14, rubber head; 15, reaction solution; 16, connection; 17, cotton swab;

具体实施方式Detailed ways

以下结合附图1-4对本发明作进一步详细说明。The present invention will be described in further detail below in conjunction with accompanying drawings 1-4.

实施例1：一种活体动物疫病抗原抗体快速检测仪，如图1-4所示，包括检测试剂盒、采样棉签和样本处理试管，所述检测试剂盒包括底板1、盖板2、镜片3和试剂条9，所述底板1与盖板2固定连接，所述底板1上设有固定区6，所述固定区6下端连接有传递区7，所述固定区6内置有试剂条9，所述试剂条9尾部与传递区7相连，所述盖板2上设有结果显示区8，所述结果显示区8表面覆盖镜片3，所述镜片3与盖板2焊接，所述结果显示区8中心处设有显示槽4，所述盖板2靠近下边缘处设有加样区5，所述加样区5与传递区7连通。所述试剂条9为抗原检测试剂条9，所述抗原检测试剂条9采用荧光标记显色。Embodiment 1: A kind of rapid detection instrument of antigen antibody of living animal epidemic disease, as shown in Figure 1-4, comprises detection kit, sampling cotton swab and sample processing test tube, and described detection kit comprisesbottom plate 1,cover plate 2,lens 3 and thereagent strip 9, thebase plate 1 is fixedly connected with thecover plate 2, thebase plate 1 is provided with a fixedarea 6, the lower end of the fixedarea 6 is connected with atransfer area 7, and the fixedarea 6 has a built-inreagent strip 9, The tail of thereagent strip 9 is connected to thetransfer area 7, thecover plate 2 is provided with a result display area 8, the surface of the result display area 8 covers thelens 3, thelens 3 is welded to thecover plate 2, and the result displayA display groove 4 is provided at the center of the area 8 , and a sample loading area 5 is provided near the lower edge of thecover plate 2 , and the sample loading area 5 communicates with thetransfer area 7 . Thereagent strip 9 is an antigendetection reagent strip 9, and the antigendetection reagent strip 9 adopts a fluorescent marker for color development.

所述采样棉签包括棉棒17和棉球19，所述棉棒17底部设有易折区18，所述易折区18底部设有棉球19。所述棉球19由亲水性纤维和疏水性材料制成，所述亲水性纤维占比为30％-70％,所述亲水性纤维采用蚕丝、兔毛、驼毛、棉、麻、粘胶纤维，所述疏水性材料可选择涤纶、锦纶、氨纶、丙纶、氯纶、尼龙。The sampling cotton swab includes acotton swab 17 and acotton ball 19 , the bottom of thecotton swab 17 is provided with aneasy-fold area 18 , and the bottom of theeasy-fold area 18 is provided with acotton ball 19 . Thecotton ball 19 is made of hydrophilic fibers and hydrophobic materials, the proportion of the hydrophilic fibers is 30%-70%, and the hydrophilic fibers are silk, rabbit hair, camel hair, cotton, hemp , viscose fiber, the hydrophobic material can choose polyester, nylon, spandex, polypropylene fiber, polyvinyl chloride, nylon.

所述样本处理试管包括试剂管10、汇流区11和橡胶头14，所述试剂管10底部固定连接汇流区11，所述试剂管10与汇流区11之间设有压力阀13，所述汇流区11底部设有塑料球12，所述橡胶头14底部设有连接处16，所述连接处16与试剂管10螺纹连接，所述试剂管10内置有反应液15。The sample processing test tube includes areagent tube 10, aconfluence area 11 and arubber head 14. The bottom of thereagent tube 10 is fixedly connected to theconfluence area 11, and apressure valve 13 is arranged between thereagent tube 10 and theconfluence area 11. Aplastic ball 12 is provided at the bottom of thearea 11, and a joint 16 is provided at the bottom of therubber head 14, and the joint 16 is screwed to thereagent tube 10, and thereagent tube 10 contains areaction solution 15 inside.

所述反应液15中包含生理盐水、非离子型表面活性剂和防腐剂，所述非离子型表面活性剂可采用TritonX-100、月桂醇聚氧乙烯醚、伯醇聚氧乙烯醚、仲醇聚氧乙烯醚、格尔伯特醇聚氧乙烯醚、辛醇聚氧乙烯醚、异辛醇聚氧乙烯醚；所述防腐剂可采用ProClin300或者叠氮化钠。其中生理盐水含量在99.5％-99.8％，非离子型表面活性剂以及防腐剂含量均在千分之一。所述反应液15pH值在6.5-7.5之间，通过磷酸二氢钾和磷酸氢二钠配置的磷酸缓冲液调节pH值，也可使用PBS缓冲液调节pH。Include physiological saline, nonionic surfactant and preservative in the describedreaction solution 15, described nonionic surfactant can adopt TritonX-100, lauryl polyoxyethylene ether, primary alcohol polyoxyethylene ether, secondary alcohol Polyoxyethylene ether, Guerbet alcohol polyoxyethylene ether, octanol polyoxyethylene ether, isooctyl alcohol polyoxyethylene ether; the preservative can use ProClin300 or sodium azide. The content of physiological saline is 99.5%-99.8%, and the content of non-ionic surfactant and preservative is 1/1000. The pH value of thereaction solution 15 is between 6.5-7.5, and the pH value is adjusted by a phosphate buffer solution prepared with potassium dihydrogen phosphate and disodium hydrogen phosphate, and the pH value can also be adjusted by using a PBS buffer solution.

实施例2：本实施例与实施例1的不同之处在于试剂条9为抗体检测试剂条9，抗体检测试剂条9通过荧光标记显色。Embodiment 2: The difference between this embodiment andEmbodiment 1 is that thereagent strip 9 is an antibodydetection reagent strip 9, and the antibodydetection reagent strip 9 is colored by a fluorescent label.

工作原理：本发明中采用采样棉签对养殖动物的自然短腔道液体样本，如口腔分泌液、尿道口残留液、粪便、乳汁；或创面液体样本，如血液、脓性或非脓性分泌液。将采集到的样本放入反应液15中，在反应液15中完成洗脱后，将其转移至检测试剂盒内，与试剂条9反应，通过荧光显色判断是否存在某种病毒的抗体或者抗原，试剂条9中有待检测抗原或抗体对应的标志物。生产时可根据常发的动物疫病，针对性的生产一系列的试剂条9，每一种疫病存在其相应的抗原试剂条9和抗体试剂条9，需要检测动物是否存在某种抗原时，农户购买相应抗原试剂条9的检测试剂盒，采样、处理再检测即可，需要检测是否存在某种抗体时，同样购买相应的抗体检测试剂盒即可，采样棉签和样本处理试管均通用。Working principle: In the present invention, sampling cotton swabs are used to sample natural short cavity liquid samples of farmed animals, such as oral secretions, urethral residual fluid, feces, milk; or wound liquid samples, such as blood, purulent or non-purulent secretions . Put the collected sample into thereaction solution 15, transfer it to the detection kit after the elution in thereaction solution 15, react with thereagent strip 9, and determine whether there is an antibody to a certain virus or Antigen, the marker corresponding to the antigen or antibody to be detected is contained in thereagent strip 9 . During production, a series ofreagent strips 9 can be produced in a targeted manner according to the frequently occurring animal diseases. Each disease has its correspondingantigen reagent strip 9 andantibody reagent strip 9. When it is necessary to detect whether there is a certain antigen in the animal, the farmer can Purchase the detection kit of the correspondingantigen reagent strip 9, take samples, process and then detect. When it is necessary to detect whether there is a certain antibody, you can also purchase the corresponding antibody detection kit. Sampling swabs and sample processing test tubes are common.

本具体实施例仅仅是对本发明的解释，其并不是对本发明的限制，本领域技术人员在阅读完本说明书后可以根据需要对本实施例做出没有创造性贡献的修改，但只要在本发明的权利要求范围内都受到专利法的保护。This specific embodiment is only an explanation of the present invention, and it is not a limitation of the present invention. Those skilled in the art can make modifications to this embodiment without creative contribution as required after reading this specification, but as long as they are within the rights of the present invention All claims are protected by patent law.

Claims

1. A rapid detector for antigen and antibody of living animal epidemic disease is characterized in that: including detect reagent box, sampling cotton swab and sample processing test tube, detect reagent box includes bottom plate (1), apron (2), lens (3) and reagent strip (9), bottom plate (1) and apron (2) fixed connection, be equipped with fixed zone (6) on bottom plate (1), fixed zone (6) lower extreme is connected with transfer district (7), fixed zone (6) embeds has reagent strip (9), reagent strip (9) afterbody links to each other with transfer district (7), be equipped with result display area (8) on apron (2), result display area (8) surface cover lens (3), lens (3) and apron (2) welding, result display area (8) center department is equipped with display groove (4), apron (2) are close to bottom edge department and are equipped with application of sample district (5), application of sample district (5) and transfer district (7) intercommunication.

2. The rapid detector for antibodies against live animal epidemic disease antigen according to claim 1, wherein: the sampling cotton swab comprises a cotton swab (17) and a cotton ball (19), wherein an easy-to-fold area (18) is arranged at the bottom of the cotton swab (17), and the cotton ball (19) is arranged at the bottom of the easy-to-fold area (18).

3. The rapid detector for antibodies against live animal epidemic disease antigen according to claim 1, wherein: sample processing test tube includes reagent pipe (10), collection flow area (11) and rubber head (14), reagent pipe (10) bottom fixed connection collection flow area (11), be equipped with pressure valve (13) between reagent pipe (10) and collection flow area (11), collection flow area (11) bottom is equipped with plastic ball (12), rubber head (14) bottom is equipped with junction (16), junction (16) and reagent pipe (10) threaded connection, reagent pipe (10) embeds has reaction liquid (15).

4. The rapid detector for antibodies against live animal epidemic disease antigen according to claim 1, wherein: the reagent strip (9) adopts fluorescent marking for color development; the reagent strips (9) are divided into antigen detection reagent strips (9) and antibody detection reagent strips (9).

5. The rapid detector for antibodies against live animal epidemic disease antigen according to claim 3, wherein: the reaction solution (15) comprises normal saline, a nonionic surfactant and a preservative, wherein the nonionic surfactant can be Triton X-100, laureth, primary alcohol polyoxyethylene ether, secondary alcohol polyoxyethylene ether, guerbet alcohol polyoxyethylene ether, octanol polyoxyethylene ether and isooctanol polyoxyethylene ether; the preservative can be ProClin300 or sodium azide; wherein the physiological saline content is 99.5% -99.8%, and the nonionic surfactant and the preservative content are all in thousandth.

6. The rapid detector for antibodies against live animal epidemic disease antigen according to claim 3, wherein: the pH value of the reaction solution (15) is between 6.5 and 7.5, and the pH value is regulated by a phosphate buffer solution prepared from potassium dihydrogen phosphate and disodium hydrogen phosphate.

7. The rapid detector for antibodies against live animal epidemic disease antigen according to claim 2, wherein: the cotton ball (19) is made of hydrophilic fibers and hydrophobic materials, the ratio of the hydrophilic fibers is 30% -70%, the hydrophilic fibers are silk, rabbit hair, camel hair, cotton, hemp and viscose fibers, and the hydrophobic materials can be selected from terylene, chinlon, spandex, polypropylene, chlorfiber and nylon.