CN115591004A - Application of yam mucus glue in dressing, wound dressing and preparation method thereof - Google Patents
Application of yam mucus glue in dressing, wound dressing and preparation method thereofDownload PDFInfo
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Abstract
Description
Translated fromChinese技术领域technical field
本发明属于生物材料技术领域，涉及天然生物材料的医药用途，具体涉及一种山药黏液胶在敷料中的应用、伤口敷料及其制备方法。The invention belongs to the technical field of biological materials, and relates to the medical application of natural biological materials, in particular to the application of yam mucus glue in dressings, wound dressings and a preparation method thereof.
背景技术Background technique
在创伤救治中，伤口敷料是一种必不可少的医疗器械，通过对伤口的包覆，实现止血并形成物理屏障，降低创面感染几率，减少渗出，为创面修复创造适宜的局部环境。特别是在院外环境创伤急救过程中，性能优良的伤口敷料有利于提高急救效果。对于开放伤口来说，快速封闭伤口尤为重要。手术缝合、胶贴和医用皮肤牵张闭合器等是目前最常用的封闭伤口的方法，但并非通用手段，各自仅有一些适用场景。In trauma treatment, wound dressing is an essential medical device. By covering the wound, it can achieve hemostasis and form a physical barrier, reduce the chance of wound infection, reduce exudation, and create a suitable local environment for wound repair. Especially in the process of trauma first aid in the out-of-hospital environment, wound dressings with excellent performance are conducive to improving the first aid effect. Rapid wound closure is especially important for open wounds. Surgical sutures, adhesive tapes, and medical skin stretch closures are currently the most commonly used methods for closing wounds, but they are not universal methods, and each has only some applicable scenarios.
使用黏合剂粘黏是一种方便封闭伤口的方法。目前，人工合成黏合剂如氰基丙烯酸酯、聚甲基丙烯酸甲酯、聚乙二醇(PEG)、聚氨酯等，由于其黏附力强且易于功能化，虽然被广泛应用于临床。但是，一些并发症限制了其应用，如生物相容性较差，其降解产物可引起炎症甚至致癌等。一些生物来源的天然粘性成分，除了具有良好的黏附性能，它们还具备良好的生物相容性和生物可降解性，因此基于天然材料的医用组织黏合剂已成为人们关注的热点。Adhesives are a convenient way to close wounds. At present, synthetic adhesives such as cyanoacrylate, polymethyl methacrylate, polyethylene glycol (PEG), polyurethane, etc., are widely used clinically because of their strong adhesion and easy functionalization. However, some complications limit its application, such as poor biocompatibility, and its degradation products can cause inflammation and even cancer. Some natural adhesive components from biological sources, in addition to good adhesive properties, also have good biocompatibility and biodegradability, so medical tissue adhesives based on natural materials have become a hot spot of attention.
山药是薯蓣科植物薯蓣(Dioscorea opposita Thunb.)的干燥根茎，始载于《神农本草经》，为常见的大宗中药材，亦是传统的药食同源植物，具有增强免疫力、调节肠胃、降血糖、抗衰老、抗肿瘤等功能。新鲜山药富含黏液质及多糖，山药黏液不仅具有良好的乳化和流变性，常用于制备乳剂，膜剂和凝胶剂，还具有多种生物活性，如抗氧化性，免疫增强活性、降压作用、抗菌、抗肿瘤、降血脂作用等。现有对山药药用价值的研究主要基于中药食疗的特点，通过研究口服给药发挥其药学作用。本发明提供山药黏液在伤口治疗方面的外科学新用途。Yam is the dry rhizome of Dioscorea opposita Thunb., which was first recorded in "Shen Nong's Materia Medica". It is a common bulk Chinese medicinal material and a traditional medicinal and edible plant. Hypoglycemic, anti-aging, anti-tumor and other functions. Fresh yam is rich in mucus and polysaccharides. Yam mucus not only has good emulsification and rheological properties, it is often used to prepare emulsions, films and gels, and also has a variety of biological activities, such as antioxidant, immune enhancement activity, antihypertensive Antibacterial, antitumor, hypolipidemic, etc. The existing research on the medicinal value of yam is mainly based on the characteristics of traditional Chinese medicine diet therapy, and its pharmaceutical effect is exerted through the study of oral administration. The invention provides a new surgical application of yam mucus in wound treatment.
发明内容Contents of the invention
有鉴于此，本发明的目的之一在于提供一种山药黏液胶的用途。In view of this, one of the purposes of the present invention is to provide a kind of purposes of yam slime glue.
其技术方案如下：Its technical scheme is as follows:
山药黏液胶在制备组织粘合剂或伤口敷料中的应用，所述山药黏液胶由山药黏液浓缩形成或由山药黏液干粉复溶形成。The application of the yam mucus glue in preparing tissue adhesive or wound dressing, wherein the yam mucus glue is formed by concentrating yam mucus or redissolving yam mucus dry powder.
本发明的目的之二在于提供一种基于山药黏液的伤口敷料。The second object of the present invention is to provide a wound dressing based on yam mucus.
其技术方案如下：Its technical scheme is as follows:
一种基于山药黏液的伤口敷料，其关键在于，包括多孔敷料基体，该多孔敷料基体上涂覆有山药黏液胶。The key point of a wound dressing based on yam mucus is that it includes a porous dressing base, and the porous dressing base is coated with yam mucus glue.
作为优选，上述多孔敷料基体为PLGA静电纺薄膜。Preferably, the porous dressing base is a PLGA electrospun film.
作为优选，以山药黏液干粉计，上述多孔敷料基体表面的山药黏液胶涂覆量为25～50mg/cm2。Preferably, based on the dry powder of yam mucus, the coating amount of yam mucus glue on the surface of the porous dressing base is 25-50 mg/cm2 .
本发明的目的之三在于提供一种伤口敷料的制备方法。The third object of the present invention is to provide a preparation method of wound dressing.
其技术方案如下：Its technical scheme is as follows:
一种如上所述伤口敷料的制备方法，其关键在于制备过程为：分别制备所述多孔敷料基体和山药黏液胶，再将所述山药黏液胶均匀涂覆于所述多孔敷料基体上，灭菌后得到所述伤口敷料；A method for preparing a wound dressing as described above, the key of which is that the preparation process is as follows: respectively prepare the porous dressing base and the yam mucus glue, then apply the yam mucus glue evenly on the porous dressing base, sterilize After obtaining the wound dressing;
所述山药黏液胶中，以山药黏液干粉计其浓度为2～4mg/μL。In the yam mucus glue, the concentration of the yam mucus dry powder is 2-4 mg/μL.
作为优选，上述山药黏液胶由山药黏液干粉加水复溶调制而成，所述山药黏液干粉由新鲜山药破碎后分离汁液，再将所述汁液干燥得到。Preferably, the above-mentioned yam mucilage glue is prepared by reconstitution of yam mucilage dry powder with water, and the yam mucilage dry powder is obtained by crushing fresh yam, separating the juice, and then drying the juice.
作为优选，上述干燥方法为常压加热干燥、减压干燥、喷雾干燥、沸腾干燥、真空冷冻干燥中的任意一种。Preferably, the above-mentioned drying method is any one of heating drying at normal pressure, drying under reduced pressure, spray drying, boiling drying, and vacuum freeze drying.
本发明的目的之四在于提供一种伤口敷料包。The fourth object of the present invention is to provide a wound dressing pack.
其技术方案如下：Its technical scheme is as follows:
一种伤口敷料包，其关键在于包括灭菌后分别独立封装的所述多孔基体、山药黏液干粉以及生理盐水。A wound dressing package, the key of which is to include the porous matrix, yam mucus dry powder and physiological saline which are individually packaged after sterilization.
作为优选，上述山药黏液干粉与生理盐水的比例为(2～4)mg:1μL。Preferably, the ratio of the above-mentioned yam mucus dry powder to physiological saline is (2-4) mg:1 μL.
附图说明Description of drawings
图1为实施例1制备的山药黏液干粉(1A)、山药黏液干粉复溶胶(1B)的外观照片，以及山药黏液干粉的扫描电子显微镜图片(1C)和实施例2制备的PLGA薄膜的扫描电子显微镜图片(1D)；Fig. 1 is the appearance photo of the yam mucus dry powder (1A) and the yam mucus dry powder resolvation gel (1B) prepared in Example 1, and the scanning electron microscope picture (1C) of the yam mucus dry powder and the scanning electron of the PLGA film prepared in Example 2 Microscope picture (1D);
图2为山药黏液复溶胶的黏稠外观(1A)及黏合性负重测试(2B)；Figure 2 shows the viscous appearance (1A) and the adhesive load test (2B) of the yam mucus reconstituted gel;
图3为山药黏液注射于大鼠皮下的降解实验，其中：(3A)注射示意图；(3B)注射部位形成的隆起和皮下包块外观照片；(3C)注射部位组织HE染色图；Figure 3 is the degradation experiment of yam mucus injected subcutaneously in rats, wherein: (3A) schematic diagram of injection; (3B) photo of the appearance of bulge and subcutaneous mass formed at the injection site; (3C) HE staining diagram of tissue at the injection site;
图4为山药黏液胶-PLGA复合膜作为伤口敷料实验图片，其中：(4A)山药黏液胶-PLGA复合膜的制备及用于伤口黏合；(4B)经不同处理方式处理后实验兔伤口愈合过程；Figure 4 is the experimental picture of the yam mucus glue-PLGA composite film as a wound dressing, in which: (4A) the preparation of the yam mucus glue-PLGA composite film and its use in wound adhesion; (4B) the experimental rabbit wound healing process after different treatments ;
图5为经不同处理方式处理后实验兔皮肤伤口组织HE染色图片。Figure 5 is the HE staining pictures of experimental rabbit skin wound tissue after different treatment methods.
具体实施方式detailed description
以下结合实施例和附图对本发明作进一步说明。The present invention will be further described below in conjunction with embodiment and accompanying drawing.
山药黏液的提取及干燥Extraction and drying of yam mucus
实施例1Example 1
新鲜山药购自超市，本实验所用山药品种为小白嘴山药。Fresh yams were purchased from supermarkets, and the variety of yam used in this experiment was Xiaobaizui yam.
将山药洗净泥沙，去皮，采用家用榨汁机榨取山药黏液，4层医用纱布滤去残渣。部分滤液采用真空冷冻干燥得到山药黏液干粉。部分滤液采用减压干燥浓缩，得到几种不同浓度的山药黏液胶，以山药黏液干粉计，其浓度分别为2mg/μL、3mg/μL和4mg/μL。Wash the yam from the sediment, peel off the skin, extract the yam mucus with a household juicer, and filter out the residue with 4 layers of medical gauze. Part of the filtrate was vacuum freeze-dried to obtain dry powder of yam mucilage. Part of the filtrate was dried and concentrated under reduced pressure to obtain several different concentrations of yam mucilage glue, the concentrations of which were 2 mg/μL, 3 mg/μL and 4 mg/μL in terms of yam mucilage dry powder.
山药黏液胶-PLGA复合膜制备Preparation of yam mucilage glue-PLGA composite film
实施例2Example 2
首先采用静电纺丝法制备聚乳酸-羟基乙酸共聚物(PLGA)薄膜，PLGA购自Sigma公司，分子量为1,200，两种共聚物单体乳酸和羟基乙酸的聚合质量比为75:25。取PLGA溶解于二氯甲烷中，配置成0.25g/mL的聚合物溶液。将聚合物溶液盛装于注射器，使用微量注射泵推注出液，设置注射器喷头与接收器之间的距离为15cm，溶液流速为0.5mL/min，纺丝完成后，干燥得到PLGA薄膜。Firstly, poly(lactic-co-glycolic acid) (PLGA) film was prepared by electrospinning. PLGA was purchased from Sigma Company with a molecular weight of 1,200. The mass ratio of the two copolymer monomers, lactic acid and glycolic acid, was 75:25. Dissolve PLGA in dichloromethane to form a 0.25 g/mL polymer solution. Put the polymer solution in a syringe, use a micro-syringe pump to push out the liquid, set the distance between the nozzle of the syringe and the receiver to 15cm, and the flow rate of the solution to 0.5mL/min. After spinning, dry to obtain a PLGA film.
将PLAG薄膜剪裁成2.5cm×2cm的膜片。分别取实施例1制备的三种山药黏液胶各60μL，均匀涂抹于膜片一侧上，即得三种山药黏液胶-PLGA复合膜，以山药黏液干粉计，三种复合膜上的山药黏液胶的涂覆量分别为24mg/cm2、36mg/cm2和48mg/cm2。Cut the PLAG film into a 2.5cm×2cm membrane. Take 60 μL of each of the three kinds of yam mucus glue prepared in Example 1, and apply them evenly on one side of the membrane to obtain three kinds of yam mucus glue-PLGA composite films. The coating amounts of glue were 24 mg/cm2 , 36 mg/cm2 and 48 mg/cm2 , respectively.
实施例3Example 3
按照实施例2的方法制备2.5cm×2cm的PLAG膜片。A 2.5 cm x 2 cm PLAG membrane was prepared according to the method in Example 2.
取实施例1制备的山药黏液干粉200mg，加入100μL生理盐水调制成均匀粘稠胶状，即得2mg/μL的山药黏液复溶胶，然后将其均匀涂抹于膜片上，同样得到山药黏液胶-PLGA复合膜。以山药黏液干粉计，复合膜上的山药黏液复溶胶的涂覆量为40mg/cm2Take 200 mg of the dry powder of yam mucus prepared in Example 1, add 100 μL of normal saline to make a uniform viscous gel, and then obtain 2 mg/μL of yam mucus reconstituted gel, and then apply it evenly on the diaphragm to obtain yam mucus glue- PLGA composite film. Based on the dry powder of yam mucus, the coating amount of yam mucus resolubilization gel on the composite film is 40mg/cm2
实施例4Example 4
按照实施例2的方法制备2.5cm×2cm的PLAG膜片。A 2.5 cm x 2 cm PLAG membrane was prepared according to the method in Example 2.
取实施例1制备的山药黏液干粉200mg，加入66μL生理盐水调制成均匀粘稠胶状，即得3mg/μL的山药黏液复溶胶，然后将其均匀涂抹于膜片上，得到山药黏液胶-PLGA复合膜。Take 200 mg of the dry powder of yam mucus prepared in Example 1, add 66 μL of normal saline to make a uniform viscous gel, and then obtain 3 mg/μL of yam mucus reconstituted gel, and then apply it evenly on the diaphragm to obtain yam mucus glue-PLGA Composite film.
实施例5Example 5
按照实施例2的方法制备2.5cm×2cm的PLAG膜片。A 2.5 cm x 2 cm PLAG membrane was prepared according to the method in Example 2.
取实施例1制备的山药黏液干粉200mg，加入50μL生理盐水调制成均匀粘稠胶状，即得4mg/μL的山药黏液复溶胶，然后将其均匀涂抹于膜片上，得到山药黏液胶-PLGA复合膜。Take 200 mg of the dry powder of yam mucus prepared in Example 1, add 50 μL of physiological saline to make a uniform viscous gel, and obtain 4 mg/μL of yam mucus reconstituted gel, and then apply it evenly on the diaphragm to obtain yam mucus glue-PLGA Composite film.
山药黏液干粉及PLGA薄膜形貌Morphology of Yam Mucus Dry Powder and PLGA Film
如图1A，采用冷冻干燥制得的山药黏液干粉呈灰白色。山药黏液干粉加水复溶能调制成凝胶状，如图1B所示。表明，采用冷冻干燥法制备的山药黏液干粉，其胶粘性得到保持。本领域技术人员容易理解，采用其他干燥方法如减压干燥、喷雾干燥、沸腾干燥等，只要不破坏其化学结构，也能保持复溶胶的胶粘性。As shown in Figure 1A, the dry powder of yam mucus obtained by freeze-drying is off-white. Yam mucus dry powder can be reconstituted into a gel by adding water, as shown in Figure 1B. It shows that the stickiness of yam mucus dry powder prepared by freeze-drying method is maintained. Those skilled in the art can easily understand that other drying methods, such as vacuum drying, spray drying, boiling drying, etc., can also maintain the adhesiveness of the resolubilized gel as long as the chemical structure is not destroyed.
采用常规方法制样，将山药黏液干粉和PLGA薄膜置于扫描电子显微镜下(SEM,Hitachi S-3400N)观察。如图1C所示，扫描电镜下，山药黏液干粉显微形态为不规则颗粒状。如图1D所示，PLGA薄膜为质地均一的纤维网状结构。Samples were prepared by conventional methods, and the dry powder of yam mucus and PLGA film were observed under a scanning electron microscope (SEM, Hitachi S-3400N). As shown in Figure 1C, under the scanning electron microscope, the microscopic morphology of the dried yam mucus powder is irregular granular. As shown in Figure 1D, the PLGA film has a uniform fiber network structure.
山药黏液胶粘性测试Adhesive test of yam mucus
按照实施例4制备山药黏液复溶胶，如图2A所示，该复溶胶表现出强的粘黏性。将复溶胶涂覆于两块载玻片之间，其中一块载玻片的一端固定，另一块载玻片下悬挂重物，能够负重0.5kg。将涂覆有复溶胶的一块盖玻片与丁腈手套粘连一起，亦能负重0.2kg。这些试验表明，山药黏液复溶胶具有优异的粘连性能。The yam mucus reconstituted gel was prepared according to Example 4. As shown in FIG. 2A , the reconstituted gel showed strong viscosity. Apply the resolvation gel between two glass slides, one end of one glass slide is fixed, and a heavy object is suspended under the other glass slide, which can bear a load of 0.5 kg. Adhere a cover glass coated with resolution gel with nitrile gloves, which can also bear a load of 0.2 kg. These tests showed that the yam mucus resolubilized gel has excellent adhesion properties.
山药复溶黏液生物相容性Yam Reconstituted Mucus Biocompatibility
取实施例1制备的山药黏液干粉，灭菌后加无菌生理盐水复溶形成50mg/mL的山药复溶黏液，注射到大鼠背部皮下，注射量1mL，注射后的局部皮肤隆起。注射示意图如图3A所示。分别在第1周、4周、8周和12周拍照记录小鼠注射部位皮肤外观，处死大鼠并取注射部位皮下组织样本，拍照记录后，常规进行组织固定、石蜡切片、HE染色，进行组织病理学观察。Take the dry powder of yam mucus prepared in Example 1, sterilize it and redissolve it with sterile physiological saline to form 50 mg/mL reconstituted yam mucus, inject it into the back of rats subcutaneously, the injection volume is 1 mL, and the local skin bulges after injection. The injection schematic is shown in Figure 3A. The appearance of the skin at the injection site was photographed and recorded at 1 week, 4 weeks, 8 weeks and 12 weeks respectively. The rats were sacrificed and the subcutaneous tissue samples at the injection site were taken. Histopathological observation.
如图3B所示，随着时间延长，注射部位皮肤隆起逐渐变平，皮下组织样本也可以看到注射形成的包块逐渐减小，至12周时几乎不可见。如图3C所示，组织染色显示注射形成的囊状包块边界清晰，未发生弥散，并逐渐被吸收而减小，且炎症反应不明显，表明山药复溶黏液具有良好的生物相容性。As shown in Figure 3B, with the prolongation of time, the skin bulge at the injection site gradually flattened, and the subcutaneous tissue samples also showed that the mass formed by the injection gradually decreased, and it was almost invisible at 12 weeks. As shown in Figure 3C, histological staining showed that the cystic mass formed by injection had a clear boundary, did not diffuse, and was gradually absorbed and reduced, and the inflammatory reaction was not obvious, indicating that the reconstituted mucus of yam has good biocompatibility.
山药黏液胶-PLGA复合膜用作皮肤伤口敷料Yam mucus glue-PLGA composite film used as skin wound dressing
实施例6Example 6
实验兔背部脱毛后，使用手术刀切割制造三条长度为2cm的皮肤伤口，建立伤口模型。三处伤口处理方式分别为：(a)使用山药黏液胶-PLGA复合膜进行粘合，山药黏液胶-PLGA复合膜按照实施例2中的方式制备，即先分别将山药黏液干粉和PLGA薄膜采用60Co灭菌，然后将山药黏液干粉加生理盐水调制成复溶胶，将该复溶胶直接涂抹于PLGA薄膜的一侧，形成山药黏液胶-PLGA复合膜，如图4A所示。使切口两侧皮肤相互靠近吻合，将山药黏液胶-PLGA复合膜贴覆在伤口上。(b)伤口采用常规手术线缝合。(c)伤口不作任何处理，作为对照。使用6只兔进行伤口愈合实验。实验后1～12天，多次拍照记录伤口愈合情况。同时，在实验后第1、3、5、7天分别取伤口处组织样本，常规HE染色观察伤口处组织恢复情况。After hair removal on the back of the experimental rabbit, three skin wounds with a length of 2 cm were cut with a scalpel to establish a wound model. The treatment methods of the three wounds are as follows: (a) use yam mucus glue-PLGA composite film for bonding, and the yam mucus glue-PLGA composite film is prepared according to the method in Example 2, that is, the yam mucus dry powder and the PLGA film are respectively used After sterilizing with60 Co, the dry powder of yam mucilage was mixed with normal saline to make a resolubilization gel, which was directly applied to one side of the PLGA film to form a yam mucilage glue-PLGA composite film, as shown in Figure 4A. Make the skin on both sides of the incision close to each other, and stick the yam mucus glue-PLGA composite film on the wound. (b) The wound was closed with conventional surgical sutures. (c) The wound was left untreated as a control. Six rabbits were used for wound healing experiments. From 1 to 12 days after the experiment, multiple photos were taken to record the wound healing. At the same time, wound tissue samples were taken on the 1st, 3rd, 5th, and 7th days after the experiment, and conventional HE staining was used to observe the recovery of wound tissue.
如图4A，同一实验兔背部三处伤口分别按设定方式处理。如图4B和5所示，与对照组相比，山药黏液胶-PLGA复合膜能很好地黏合伤口，且伤口愈合速度明显高于对照组，表明复合膜不仅能黏合伤口还能促进伤口愈合。实验后一天，经山药黏液胶-PLGA复合膜处理后的伤口已经闭合且后续未发现破裂，考虑到实验动物的活动并不受限，这种黏合效果能够满足一般伤口粘接要求。由此表明，山药黏液胶-PLGA复合膜具有极好地黏合伤口的作用，且粘合处理与缝合处理对伤口闭合的作用相当。粘合处理组未见皮肤红肿等皮肤刺激现象，表明山药黏液胶-PLGA复合膜体外应用的安全性。粘合处理组与缝合处理组伤口的愈合速度也接近。考虑到缝合处理还会带来缝合损伤、非降解手术缝合线还需要拆线等不利因素，本发明的山药黏液胶-PLGA复合膜作为伤口敷料的优势更为明显，具有作为伤口敷料的应用潜力。As shown in Figure 4A, the three wounds on the back of the rabbit in the same experiment were treated according to the set method. As shown in Figures 4B and 5, compared with the control group, the yam mucus glue-PLGA composite film can adhere to the wound well, and the wound healing speed is significantly higher than that of the control group, indicating that the composite film can not only adhere to the wound but also promote wound healing . One day after the experiment, the wounds treated with the yam mucus glue-PLGA composite film were closed and no rupture was found in the follow-up. Considering that the activities of the experimental animals were not restricted, this adhesive effect could meet the general wound adhesion requirements. This shows that the yam mucus glue-PLGA composite film has an excellent effect on wound adhesion, and the effect of adhesion treatment and suture treatment on wound closure is equivalent. There was no skin irritation such as skin redness and swelling in the adhesive treatment group, indicating the safety of the yam mucus glue-PLGA composite film in vitro. The wound healing speeds of the adhesive treatment group and the suture treatment group were also similar. Considering the unfavorable factors such as suture damage caused by suture treatment, non-degradable surgical sutures need to be removed, etc., the yam mucus glue-PLGA composite film of the present invention has more obvious advantages as a wound dressing, and has application potential as a wound dressing .
聚乳酸-羟基乙酸共聚物(PLGA)是由乳酸和羟基乙酸随机聚合而成的无功能侧基有机聚合物，是一类重要的生物医用高分子材料，其降解产物乳酸和羟基乙酸，也是人代谢途径的副产物，因此PLGA具有良好的生物相容性、无毒、无刺激性、无免疫原性、药物缓释等特性，广泛应用于生物医学领域，如：手术缝合线、骨折内固定材料、组织修复材料，及药物控制释放体系等。静电纺PLGA薄膜为多孔纤维膜，用于伤口敷料，不仅能够形成物理屏障减少伤口污染，且具有透气性强的优点，其良好的生物相容性也有助于创面细胞粘附迁移，促进伤口愈合。同时，静电纺PLGA薄膜作为山药黏液胶的附着基体，也能够起到抗拉作用，防止伤口处因为机体活动被拉裂。实验发现虽然单独使用山药黏液胶也可以黏合伤口，但山药黏液胶-PLGA复合膜对伤口的黏合更紧，同时便于操作，且能减少山药黏液胶直接对皮肤切口深处的接触。此外，本实验虽然是将复合膜用于皮肤切割伤的治疗，但因为材料本身良好的生物相容性，本发明的复合膜也可以用于皮肤缺损伤的治疗。Polylactic acid-glycolic acid copolymer (PLGA) is a non-functional organic polymer with side groups formed by random polymerization of lactic acid and glycolic acid. It is an important class of biomedical polymer materials. Its degradation products lactic acid and glycolic acid are also human It is a by-product of metabolic pathways, so PLGA has good biocompatibility, non-toxic, non-irritating, non-immunogenic, drug sustained release and other characteristics, and is widely used in biomedical fields, such as: surgical sutures, internal fixation of fractures materials, tissue repair materials, and drug controlled release systems, etc. Electrospun PLGA film is a porous fibrous film used in wound dressings. It can not only form a physical barrier to reduce wound pollution, but also has the advantages of strong air permeability. Its good biocompatibility also helps wound cells adhere and migrate, and promote wound healing. . At the same time, the electrospun PLGA film, as the attachment matrix of the yam mucus glue, can also play a tensile role, preventing the wound from being pulled apart due to body activities. The experiment found that although the yam mucus glue alone can also bond the wound, the yam mucus glue-PLGA composite film adheres to the wound more tightly, is easy to operate, and can reduce the direct contact of the yam mucus glue to the deep part of the skin incision. In addition, although the composite membrane was used in the treatment of skin cuts in this experiment, the composite membrane of the present invention can also be used in the treatment of skin defects due to the good biocompatibility of the material itself.
上述动物实验证明了山药黏液胶-PLGA复合膜对伤口的黏合以及促进伤口愈合的效果。为更稳定地保存，将山药黏液胶涂覆在PLGA薄膜上，再干燥得到山药黏液干粉-PLGA复合膜，灭菌后密封保存，可以延长其有效期。此外，还可以将灭菌后的PLGA薄膜、山药黏液干粉和无菌生理盐水分别封装，形成敷料包。使用时，配制山药黏液复溶胶，再涂覆在PLGA薄膜，形成山药黏液胶-PLGA复合膜。这种山药黏液胶-PLGA复合膜尤其适用于伤口的闭合止血，少量伤口渗液对复合膜性能基本无影响。The above animal experiments proved the effect of yam mucus glue-PLGA composite film on wound adhesion and promotion of wound healing. For more stable preservation, the yam mucilage glue was coated on the PLGA film, and then dried to obtain the yam mucilage dry powder-PLGA composite film, which was sealed and stored after sterilization to prolong its validity period. In addition, the sterilized PLGA film, dry powder of yam mucus and sterile saline can also be packaged separately to form a dressing pack. When in use, the yam mucus resolubilization glue is prepared, and then coated on the PLGA film to form a yam mucus glue-PLGA composite film. The yam mucus glue-PLGA composite film is especially suitable for closing and hemostasis of wounds, and a small amount of wound exudate has basically no effect on the performance of the composite film.
本发明的山药黏液胶-PLGA复合膜充分利用山药黏液胶的粘性和PLGA膜的特性，不仅发挥了山药黏液胶的粘性、无毒、良好的生物相容性等特点，有效对皮肤黏合，促进伤口愈合，且与人工合成的黏合剂相比，制备工艺简单，材料来源丰富，绿色环保，因此具有临床实用价值。The yam mucus glue-PLGA composite film of the present invention makes full use of the viscosity of the yam mucus glue and the characteristics of the PLGA film, not only brings into play the characteristics of the stickiness, non-toxicity, and good biocompatibility of the yam mucus glue, but also effectively adheres to the skin and promotes Wound healing, and compared with artificially synthesized adhesives, the preparation process is simple, the source of materials is abundant, and it is environmentally friendly, so it has clinical practical value.
本发明的有益效果：Beneficial effects of the present invention:
(1)将天然来源的生物材料用于组织黏合和伤口敷料，扩展了山药的医药用途，为山药这一口服中药材的开发利用提供新的方向；(1) The use of biomaterials from natural sources for tissue adhesion and wound dressing expands the medicinal use of yam and provides a new direction for the development and utilization of yam, an oral Chinese herbal medicine;
(2)山药黏液胶-PLGA复合膜具有优异的组织粘接性能，能够迅速闭合伤口并促进伤口愈合，且PLGA膜具有抗拉作用，能增强山药黏液胶的黏合作用，同时PLGA膜为伤口提供透气物理屏障以及作为伤口愈合的支架；(2) The yam mucus glue-PLGA composite film has excellent tissue adhesive properties, can quickly close the wound and promote wound healing, and the PLGA film has a tensile effect, which can enhance the adhesion of the yam mucus glue, and the PLGA film provides wound protection. Breathable physical barrier and as a scaffold for wound healing;
(3)采用山药黏液干粉作为保存形式，复溶后形成山药黏液胶，保留了山药黏液的粘连性能，还可控制山药黏液胶的含水量，使制备的山药黏液胶-PLGA复合膜批次均一；(3) Yam mucus dry powder is used as the preservation form, and yam mucus glue is formed after reconstitution, which retains the adhesion properties of yam mucus glue, and can also control the water content of yam mucus glue, so that the prepared yam mucus glue-PLGA composite film is uniform in batches ;
(4)山药黏液胶的材料来自常见食材，具有取材方便、天然环保、成本低廉的特点。(4) The material of yam mucilage glue comes from common food materials, which has the characteristics of convenient material acquisition, natural environmental protection and low cost.
最后需要说明的是，上述描述仅仅为本发明的优选实施例，本领域的普通技术人员在本发明的启示下，在不违背本发明宗旨及权利要求的前提下，可以做出多种类似的表示，这样的变换均落入本发明的保护范围之内。Finally, it should be noted that the above description is only a preferred embodiment of the present invention, and those of ordinary skill in the art can make a variety of similar implementations under the inspiration of the present invention without violating the purpose and claims of the present invention. It means that such transformations all fall within the protection scope of the present invention.
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