Multi-degree-of-freedom electrodeless adjusting device for surface plasma resonance detectorTechnical Field
The invention relates to the field of surface plasma resonance detection, in particular to a multi-degree-of-freedom electrodeless adjusting device for a surface plasma resonance detector.
Background
The surface plasmon resonance detection technique (Surface Plasmon Resonance, SPR) is a novel biosensing analysis technique based on the SPR principle. This technique is a complex optical phenomenon, as shown in fig. 1, because the parallel polarization component of the incident light is totally internally reflected at the interface between the glass and the gold film, an evanescent wave is generated, which enters the gold film and excites free electrons therein to generate a surface plasmon wave (Surface Plasmon Wave, SPW). When incident light propagates from an optically dense medium (glass prism) to an optically sparse medium (air) at a certain angle, if the incident angle is larger than a critical angle, refracted light rays disappear, and a total reflection phenomenon occurs. The light wave can penetrate one wavelength of the photophobic medium during total reflection, then flow about half wavelength along the interface, and then return to the photophobic medium. The wave flowing along the interface is called the evanescent wave. If a layer of gold film is plated on the surface of the glass prism, free electron movement existing on the surface of the gold film can form surface plasma, and the plasma can vibrate under the electromagnetic interference to form plasma waves. When the incident light is emitted to the interface of the glass prism and the gold film at an incident angle larger than a critical angle, evanescent waves formed by total reflection of the incident light and plasma waves on the surface of the gold film can resonate under a certain condition (proper wavelength or incident angle), most of energy of the incident light is absorbed by the plasma waves during resonance, and the energy of the reflected light is sharply reduced. Under the condition that the wavelength of the incident light is determined, under the condition that the incident angle is larger than the critical angle, the incident angle is changed, a curve of the reflected light intensity changing along with the incident angle forms a deep valley, and the abscissa position corresponding to the valley bottom is the angle of the incident angle when resonance occurs, which is called an SPR angle. The SPR angle varies with the refractive index of the gold film surface, which varies primarily in proportion to the molecular mass bound to the gold surface. Specific signals of interactions between biomolecules can thus be obtained by dynamic changes of the SPR angle during the biological reaction.
Surface plasmon resonance detection techniques have a number of characteristic parameters, of which the main ones are incident light and reflected light. When incident light irradiates the gold film and the refractive index of the interface between the gold film and the medium to be detected changes, reflected light signals are collected in real time through a computer, parameters such as the concentration, the refractive index and the like of the substance to be detected can be obtained by the SPR system, and the aim of biochemical detection is fulfilled. It can be seen that both of these characteristics are important for SPR detection, namely, the irradiation position and range of the incident light, and the receiving angle and receiving range of the reflected light.
Patent CN102095684 a discloses a multi-degree-of-freedom adjusting mechanism of an optical surface plasmon resonance biosensor. The adjusting mechanism is too simple in structure, and the following problems exist in the adjusting process:
1. the multiple degrees of freedom are adjusted simultaneously, and the multiple degrees of freedom simultaneously act on the adjusting mechanism during adjustment, so that unexpected adjustment of other degrees of freedom is inevitably carried out during adjustment of one degree of freedom.
2. The adjustment size is inaccurate, and the adjustment size cannot be accurately adjusted only by hand feeling when the adjustment is performed.
3. The lack of freedom of adjustment is not comprehensive, and the lack of freedom in practical use.
The invention aims to solve the problems of adjusting the incident angle and the incident range of incident light and adjusting the receiving angle and the receiving range of reflected light.
Disclosure of Invention
The invention aims to provide a multi-degree-of-freedom electrodeless adjusting device for a surface plasma resonance detector, which is used for solving the problem that the adjusting effect of an incident angle and an incident range and a receiving angle and a receiving range in the prior art is poor.
In order to achieve the above object, the present invention provides the following solutions:
The invention provides a multi-degree-of-freedom electrodeless adjusting device for a surface plasma resonance detector, which comprises a light source adjusting unit and a CCD adjusting unit, wherein the light source adjusting unit and the CCD adjusting unit are symmetrically arranged and are connected through a connecting piece;
The light source adjusting unit and the CCD adjusting unit are identical in structure and comprise a base, an up-and-down moving platform is arranged on the base, a front-and-back moving platform is arranged on the up-and-down moving platform, a front-and-back rotating platform is arranged on the front-and-back moving platform, an up-and-down rotating platform is arranged on the front-and-back rotating platform, a left-and-right moving platform is connected on the up-and-down rotating platform, and an optical device is rotatably arranged on the left-and-right moving platform.
Preferably, the base comprises a bottom plate, guide shafts are vertically arranged on the bottom plate, left and right moving guide rails are arranged on two sides of the bottom plate, slopes are arranged on the left and right moving guide rails, a first adjusting screw is arranged between the left and right moving guide rails, and positioning components are arranged on the side parts of the left and right moving guide rails.
Preferably, the up-down moving platform comprises an up-down moving platform body, the up-down moving platform body is slidably limited on the guide shaft, a moving slide block and a front-back moving transmission piece are arranged on the side part of the up-down moving platform body, the moving slide block is slidably limited in the slope, and the first adjusting screw acts on the moving slide block.
Preferably, the tandem mobile platform comprises a front-back mobile platform body, wherein the front-back mobile platform body is provided with a front-back mobile guide rail, the front-back mobile platform body is slidably arranged on the upper-lower mobile platform body through a guide shaft, a second adjusting screw is arranged on the side part of the front-back mobile platform body, and the second adjusting screw acts on the front-back mobile transmission part.
Preferably, the front-back rotating platform comprises a front-back rotating platform body, the front-back rotating platform body is rotatably arranged on the front-back moving platform body through a first rotating shaft, and positioning fixing screws are arranged on the front-back rotating platform body.
Preferably, a rotary scale is arranged around the first rotary shaft, and a pointer is arranged on the upper surface of the first rotary shaft.
Preferably, the up-down rotary platform comprises an up-down rotary platform body, the up-down rotary platform body is fixed on the front-back rotary platform body, and a second rotary shaft is arranged at the top of the up-down rotary platform body.
Preferably, the left and right moving platform comprises a left and right moving platform body, the left and right moving platform body is rotatably connected with the second rotating shaft, the optical device is rotatably arranged on the left and right moving platform body, a third adjusting screw is arranged on the side part of the left and right moving platform body, and the third adjusting screw acts on the optical device.
Compared with the prior art, the invention has the following beneficial technical effects:
1. The multi-degree-of-freedom electrodeless adjusting device for the surface plasma resonance detector provided by the invention totally comprises up-and-down movement, front-and-back movement, up-and-down rotation, front-and-back rotation, left-and-right movement and rotation around an optical axis, and has 6 degrees of freedom, so that the adjustable range is wider;
2. According to the multi-degree-of-freedom electrodeless adjusting device for the surface plasma resonance detector, each degree of freedom can be adjusted independently, and other degrees of freedom cannot be influenced by adjusting one degree of freedom;
3. The freedom degree adjustment of the multi-degree-of-freedom electrodeless adjustment device for the surface plasma resonance detector is stepless adjustment, can be finely adjusted, and has a self-locking function.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the principle of SPR phenomenon;
FIG. 2 is a schematic diagram of a multi-degree-of-freedom electrodeless adjusting device for a surface plasmon resonance detector;
FIG. 3 is a schematic view of a part of a light source adjusting unit of the multi-degree-of-freedom electrodeless adjusting device for the surface plasmon resonance detector;
FIG. 4 is a schematic view of a base part of the multi-degree-of-freedom electrodeless adjusting device for the surface plasmon resonance detector;
FIG. 5 is a schematic diagram of a part of a vertical moving platform of the multi-degree-of-freedom electrodeless adjusting device for the surface plasmon resonance detector;
FIG. 6 is a schematic diagram showing the up-and-down movement state of the multi-degree-of-freedom electrodeless adjusting device for the surface plasmon resonance detector;
FIG. 7 is a schematic diagram of a front-back moving platform part of the multi-degree-of-freedom electrodeless adjusting device for a surface plasmon resonance detector;
FIG. 8 is a schematic diagram of the forward and backward movement state of the multi-degree-of-freedom electrodeless adjusting device for the surface plasmon resonance detector;
FIG. 9 is a schematic diagram of a front-back rotating platform part of the multi-degree-of-freedom electrodeless adjusting device for a surface plasmon resonance detector;
FIG. 10 is a schematic diagram of the forward and backward rotation states of the multi-degree-of-freedom electrodeless adjusting device for the surface plasmon resonance detector;
FIG. 11 is a schematic diagram of a part of a vertical rotation platform of the multi-degree-of-freedom electrodeless adjusting device for a surface plasmon resonance detector;
FIG. 12 is a schematic diagram showing the left and right movement state of the multi-degree-of-freedom electrodeless adjusting device for the surface plasmon resonance detector;
FIG. 13 is a schematic view of the degree of freedom of the multiple degree of freedom electrodeless adjusting device for a surface plasmon resonance detector
In the figure, 1 is a light source adjusting unit, 11 is a base, 111 is a bottom plate, 112 is a guide shaft, 113 is a left and right moving guide rail, 114 is a first adjusting screw, 115 is a positioning component, 12 is an up and down moving platform, 121 is an up and down moving platform main body, 122 is a moving slide block, 123 is a front and back moving transmission member, 13 is a front and back moving platform, 131 is a front and back moving platform main body, 132 is a second adjusting screw, 133 is a front and back moving guide rail, 14 is a front and back rotating platform, 141 is a front and back rotating platform main body, 142 is a first rotating shaft, 15 is an up and down rotating platform, 151 is an up and down rotating platform main body, 152 is a second rotating shaft, 16 is a left and right moving platform, 161 is a third adjusting screw, 162 is a left and right moving platform main body, 2 is a CCD adjusting unit, and 3 is a connecting member.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention aims to provide a multi-degree-of-freedom electrodeless adjusting device for a surface plasma resonance detector, which is used for solving the problem that the adjusting effect of an incident angle and an incident range and a receiving angle and a receiving range in the prior art is poor.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Example 1:
The embodiment provides a multi-degree-of-freedom electrodeless adjusting device for a surface plasma resonance detector, which comprises a light source adjusting unit 1, a CCD adjusting unit 2 and a connecting piece 3 for connecting the two units, wherein the light source adjusting unit 1 and the CCD adjusting unit 2 are of the same structure and are symmetrically arranged left and right.
Specifically, taking the light source adjusting unit 1 as an example, as shown in fig. 3 and 13, the light source adjusting unit 1 includes a base 11, an up-down moving platform 12, a forward-backward moving platform 13, a forward-backward rotating platform 14, an up-down rotating platform 15, and a left-right moving platform 16 which are sequentially connected, and a light source is installed in the left-right moving platform 16, and can achieve adjustment including up-down movement, forward-backward movement, up-down rotation, forward-backward rotation, left-right movement, and rotation about an optical axis for 6 degrees of freedom.
Further, as shown in fig. 4, the base 11 includes a bottom plate 111 and a guide shaft 112, the guide shaft 112 is vertically fixed on the bottom plate 111, left and right moving rails 113 disposed on both sides of the bottom plate 111 and parallel to the bottom plate 111 and movable left and right on the bottom plate, a slowly rising slope disposed on the left and right moving rails 113, an adjusting screw 114 disposed between the left and right moving rails 113 and fixed on the bottom plate 111 and connected to the left and right moving rails 113, and a positioning member 115 for positioning.
Further, as shown in fig. 5, the up-and-down moving platform 12 includes an up-and-down moving platform body 121 on which a forward-and-backward moving transmission member 123 and a moving slider 122 are provided, both of which are respectively provided on both sides of the up-and-down moving platform body.
When the up-down movement is required, as shown in fig. 6, since the up-down moving platform 12 is placed on the guide shaft 112 of the base 11, the up-down moving platform 12 can slide up and down along the guide shaft 112, the moving slide 122 is close to the slope on the left-right moving guide rail 113, the moving slide 122 can slide along the slope on the left-right moving guide rail 113, the moving slide 122 is driven to move left and right on the bottom plate 111 by rotating the first adjusting screw 114, the moving slide 122 further drives the up-down moving platform main body 121 to move up and down along the guide shaft 112, thereby realizing up-down movement adjustment, realizing up-down adjustment by changing the spiral movement into the left-right movement and then into the up-down movement, wherein the up-down adjustment amount depends on the movement amount of the spiral movement, the up-down movement accuracy and range can be adjusted by the pitch and the length of the first adjusting screw 114, and the up-down adjustment positioning can be realized due to the self-locking property of the screw.
Further, as shown in fig. 7, the front-rear moving platform 13 includes a front-rear moving platform body 131 on which a second adjusting screw 132 and a front-rear moving rail 133 are provided.
When the adjustment of the back and forth movement is required, as shown in fig. 8, since the back and forth movement guide rail 133 of the back and forth movement platform 13 is mounted on the guide shaft 112 and is placed on the up and down movement platform 12, the second adjusting screw 132 is connected to the back and forth movement transmission member 123 on the up and down movement platform 12, at this time, the back and forth movement adjustment of the back and forth movement platform body 131 along the movement guide rail 133 can be performed by rotating the second adjusting screw 132, and similarly, the adjustment amount thereof depends on the movement amount of the spiral movement, the accuracy and the range of the back and forth movement can be adjusted by adjusting the pitch and the length of the screw, and the positioning of the back and forth adjustment can be realized due to the self-locking property of the screw.
Further, as shown in fig. 9, the front-rear rotary table 14 includes a front-rear rotary table body 141 and a first rotary shaft 142, a rotary scale is provided around the first rotary shaft 142, and a pointer is provided on the first rotary shaft 142.
When the back-and-forth rotation adjustment is required, as shown in fig. 10, since the first rotation shaft 142 of the back-and-forth rotation platform 14 is fixed on the back-and-forth movement platform 13, the angle adjustment can be performed according to the scale when the rotation is performed, and the positioning fixing is performed by the positioning fixing screw.
Further, as shown in fig. 11, the up-and-down rotary table 15 includes an up-and-down rotary table main body 151 and a second rotary shaft 152, and the up-and-down rotary table 15 is fixed to the front-and-rear rotary table 14.
Further, as shown in fig. 12, the left-right moving platform 16 includes a left-right moving platform main body 162 and a third adjusting screw 161, the left-right moving platform 16 is mounted on the up-down rotating platform 15, the light source is mounted on the left-right moving platform main body 162, and the adjusting screw 161 is mounted on the light source, at this time, the light source can be adjusted to move left and right along the left-right moving platform main body 162 by rotating the third adjusting screw 161, and similarly, the adjustment amount thereof depends on the amount of the movement of the screw movement, the accuracy and the range of the left-right movement can be adjusted by the pitch and the length of the third adjusting screw 161, and the positioning of the left-right adjustment can be realized due to the self-locking property of the screw. In addition, the left-right moving platform 16 provides another degree of freedom, namely, a degree of freedom of rotation about the optical axis.
The multi-degree-of-freedom electrodeless adjusting device for the surface plasma resonance detector provided by the invention comprises up-and-down movement, front-and-back movement, up-and-down rotation, front-and-back rotation, left-and-right movement and rotation around an optical axis, wherein the total of 6 degrees of freedom can be adjusted, the adjustable range is wider, each degree of freedom can be independently adjusted, other degrees of freedom cannot be influenced by adjusting one degree of freedom, the degree of freedom is adjusted in an electrodeless manner, precise fine adjustment can be performed, the self-locking function is realized, and the problem that the existing device is poor in adjusting effect is effectively solved.
The principles and embodiments of the present invention have been described in detail with reference to specific examples, which are provided herein to facilitate understanding of the principles and embodiments of the present invention and to provide further advantages and practical applications of the present invention, as will occur to those of skill in the art upon reading the foregoing description and the drawings. In summary, the present description should not be construed as limiting the invention.