CN114736796A - Nucleic acid detection kit with rotary valve core combination - Google Patents

Abstract

The invention discloses a nucleic acid detection kit with a rotary valve core combination, which comprises a kit body, wherein the kit body is sequentially provided with a sample reagent cavity, a first rotatable body, a first purification cavity, a second rotatable body, a second purification cavity, a third rotatable body, a reaction cavity, a fourth rotatable body, a third purification cavity and a fifth rotatable body from top to bottom, the first rotatable body is internally provided with a first immiscible phase cavity, the second rotatable body is internally provided with a second immiscible phase cavity, the third rotatable body is internally provided with a third immiscible phase cavity, the fourth rotatable body is internally provided with a fourth immiscible phase cavity, and the fifth rotatable body is internally provided with a detection cavity, the sample reagent cavity, the first immiscible phase cavity, the first purification cavity, the second immiscible phase cavity, the second purification cavity, the third immiscible phase cavity, the reaction cavity, the fourth immiscible phase cavity, the third purification cavity and the detection cavity are communicated in sequence. The kit has simple structure and reasonable design, can realize the integration of nucleic acid extraction, purification, transfer, amplification and detection, and improves the detection efficiency.

Description

Nucleic acid detection kit with rotary valve core combination

Technical Field

The invention relates to the technical field of nucleic acid detection kits, in particular to a nucleic acid detection kit with a rotary valve core combination.

Background

With the rapid development of molecular diagnostic technology, it is increasingly applied in the fields of medical and biological detection. The nucleic acid detection technology is a typical molecular diagnostic technology, and has high sensitivity, accuracy and stability due to the introduction of high sensitivity characteristics of Polymerase Chain Reaction (PCR) thermal cycling and gene in-vitro amplification, so that the high-efficiency and specific amplification and detection can be performed on pathogens or specific genes. With the increasing emergence of complicated and difficult diseases and the continuous variation of viruses, a real-time, rapid and accurate detection means is urgently needed for medical detection, and the real-time quantitative nucleic acid detection shows remarkable advantages. Once infected, the patient can accurately detect the virus in time, and the window period is short, so that valuable treatment time is seized.

Nucleic acid detection mainly involves three aspects, namely nucleic acid extraction, nucleic acid amplification and detection of amplification products. The quality and quantity of nucleic acid extraction generally directly determines the authenticity and accuracy of the final test results and test reports. For a long time, the extraction and purification of nucleic acid in a detection sample are always time-consuming and tedious processes, and the detection speed is seriously slowed down. Especially for an extremely trace biological sample, the low extraction recovery rate of trace DNA in the sample by the conventional nucleic acid extraction method is a main reason for poor subsequent PCR amplification effect. Therefore, the development of a rapid and safe nucleic acid detection kit is crucial to improving the nucleic acid detection efficiency.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a nucleic acid detection kit with a rotary valve core combination, which has a simple structure and a reasonable design, can realize integration of nucleic acid extraction, purification, transfer, amplification and real-time detection, and greatly improves the detection efficiency.

The technical scheme adopted for realizing the above purpose of the invention is as follows:

a nucleic acid detection kit with a rotary valve core combination comprises a kit body, wherein the kit body is sequentially provided with a sample reagent cavity, a first rotatable body, a first purification cavity, a second rotatable body, a second purification cavity, a third rotatable body, a reaction cavity, a fourth rotatable body, a third purification cavity and a fifth rotatable body from top to bottom, the first rotatable body, the second rotatable body, the third rotatable body, the fourth rotatable body and the fifth rotatable body are respectively and rotatably connected with the kit body, the first rotatable body is internally provided with a first immiscible phase cavity, the second rotatable body is internally provided with a second immiscible phase cavity, the third rotatable body is internally provided with a third immiscible phase cavity, the fourth rotatable body is internally provided with a fourth immiscible phase cavity, the fifth rotatable body is internally provided with a detection cavity, the sample reagent cavity, the first immiscible phase cavity, the first purification cavity, the second immiscible phase cavity, the second purification cavity, the third immiscible phase cavity and the fifth rotatable body, The third immiscible phase cavity, the reaction cavity, the fourth immiscible phase cavity, the third purification cavity and the detection cavity are communicated in sequence.

The kit body is provided with a first mounting hole, a second mounting hole, a third mounting hole, a fourth mounting hole and a fifth mounting hole from top to bottom in sequence, wherein the first rotatable body is movably connected with the first mounting hole, the second rotatable body is movably connected with the second mounting hole, the third rotatable body is movably connected with the third mounting hole, the fourth rotatable body is movably connected with the fourth mounting hole, and the fifth rotatable body is movably connected with the fifth mounting hole.

The first rotatable body, the second rotatable body, the third rotatable body, the fourth rotatable body and the fifth rotatable body are in a conical shape, the first rotatable body penetrates through the first mounting hole, the first rotatable body is in clearance fit with the first mounting hole, the second rotatable body penetrates through the second mounting hole, the second rotatable body is in clearance fit with the second mounting hole, the third rotatable body penetrates through the third mounting hole, the third rotatable body is in clearance fit with the third mounting hole, the fourth rotatable body penetrates through the fourth mounting hole, the fourth rotatable body is in clearance fit with the fourth mounting hole, the fifth rotatable body penetrates through the fifth mounting hole, and the fifth rotatable body is in clearance fit with the fifth mounting hole.

The top of the kit body is provided with a top cover for covering the sample reagent cavity, and the top cover is clamped on the kit body.

The small ends of the side walls of the first rotatable body, the second rotatable body, the third rotatable body, the fourth rotatable body and the fifth rotatable body are provided with clamp springs, the small end face of the fifth rotatable body is connected with a fifth connecting male head, and the large end faces of the first rotatable body, the second rotatable body, the third rotatable body, the fourth rotatable body and the fifth rotatable body are respectively provided with a straight handle groove.

The small end face of the first rotatable body is connected with a first connecting male head, the small end face of the second rotatable body is connected with a second connecting male head, the small end face of the third rotatable body is connected with a third connecting male head, the small end face of the fourth rotatable body is connected with a fourth connecting male head, and the small end face of the fifth rotatable body is connected with a fifth connecting male head.

The kit body is in a long and flat shape, a pair of first socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the sample reagent cavity, a pair of second socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the first purification cavity, a pair of third socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the second purification cavity, a pair of fourth socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the reaction cavity, a pair of fifth socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the third purification cavity, and the pair of first socket ear plates, the pair of second socket ear plates, the pair of third socket ear plates, the pair of fourth socket ear plates and the pair of fifth socket ear plates are distributed along a straight line;

the pair of first socket-joint ear plates, the first connecting male head, the pair of second socket-joint ear plates, the second connecting male head, the pair of third socket-joint ear plates, the third connecting male head, the pair of fourth socket-joint ear plates, the fourth connecting male head, the pair of fifth socket-joint ear plates and the fifth connecting male head are alternately distributed along a straight line.

The first socket joint ear plate, the second socket joint ear plate, the third socket joint ear plate, the fourth socket joint ear plate, the fifth socket joint ear plate, the first connection male head, the second connection male head, the third connection male head, the fourth connection male head and the fifth connection male head are all in a shape like a Chinese character 'yi', and the plane where the first connection male head, the second connection male head, the third connection male head, the fourth connection male head and the fifth connection male head are distributed is parallel to the first kit body.

The sample reagent cavity, the first purification cavity and the second purification cavity are all in a bucket shape, the first immiscible phase cavity, the second immiscible phase cavity, the third immiscible phase cavity, the reaction cavity, the fourth immiscible phase cavity, the third purification cavity and the detection cavity are all in a tubular shape, the first immiscible phase cavity and the second immiscible phase cavity are in a conical shape, a small end outlet of the sample reagent cavity is communicated with a large end inlet of the first immiscible phase cavity, a small end outlet of the first immiscible phase cavity is communicated with a large end inlet of the first purification cavity, a small end outlet of the first purification cavity is communicated with a large end inlet of the second immiscible phase cavity, a small end outlet of the second immiscible phase cavity is communicated with a large end inlet of the second purification cavity, and a small end outlet of the second purification cavity is communicated with an upper end port of the third immiscible phase cavity.

Compared with the prior art, the invention has the beneficial effects and advantages that:

1. the kit can realize the integrated operation of extraction, purification, amplification and detection of nucleic acid, is convenient and safe to operate, improves the detection efficiency, does not cause cross contamination, and improves the accuracy of measurement.

2. According to the kit, in the process of transferring nucleic acid, the magnetic beads are used for modifying the nucleic acid, and then the magnetic beads are attracted by the magnets to move so as to pull the nucleic acid to be transferred, so that the transfer efficiency is high, and the operation is convenient.

3. The kit is simple in structure, reasonable in design, compact in structure, small in occupied space, high in practicability and suitable for popularization and application in hospitals.

Drawings

FIG. 1 is a schematic diagram showing the external structure of a nucleic acid detection kit with a rotary valve cartridge assembly.

FIG. 2 is a schematic diagram of the external structure of a nucleic acid detection kit with a rotary valve core assembly (different from FIG. 1).

FIG. 3 is a schematic diagram of the internal structure of a nucleic acid detection kit with a rotary valve core assembly.

Fig. 4 is a schematic structural view of a fifth rotatable body.

Wherein, 1-kit body, 2-sample reagent cavity, 3-first rotatable body, 4-first purification cavity, 5-second rotatable body, 6-second purification cavity, 7-third rotatable body, 8-reaction cavity, 9-fourth rotatable body, 10-third purification cavity, 11-fifth rotatable body, 12-first immiscible phase cavity, 13-second immiscible phase cavity, 14-third immiscible phase cavity, 15-fourth immiscible phase cavity, 16-detection cavity, 17-first connecting male head, 18-second connecting male head, 19-third connecting male head, 20-fourth connecting male head, 21-fifth connecting male head, 22-first socket ear plate, 23-snap spring, 24-top cover and 25-handle groove.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings.

The nucleic acid detecting reagent kit with a rotary valve core assembly according to the present embodiment has a structure as shown in fig. 1, fig. 2, and fig. 3, and includes areagent kit body 1, a firstrotatable body 3, a secondrotatable body 5, a thirdrotatable body 7, a fourthrotatable body 9, and a fifthrotatable body 11, wherein thereagent kit body 1 is in a long and flat shape, and the firstrotatable body 3, the secondrotatable body 5, the thirdrotatable body 7, the fourthrotatable body 9, and the fifthrotatable body 11 are in a tapered shape. Thereagent kit body 1 is vertically arranged, and the length direction of thereagent kit body 1 is vertical to the horizontal plane. Thekit body 1 is provided with asample reagent cavity 2, a first mounting hole, afirst purification cavity 4, a second mounting hole, asecond purification cavity 6, a third mounting hole, areaction cavity 8, a fourth mounting hole, athird purification cavity 10 and a fifth mounting hole which are distributed along a straight line from top to bottom in sequence, and the plane where the axes of the first mounting hole, the second mounting hole, the third mounting hole, the fourth mounting hole and the fifth mounting hole are located is parallel to the kit body.

The top of thereagent box body 1 is provided with atop cover 24 for covering the sample reagent cavity, thetop cover 24 is clamped with the reagent box body, and various reagents are conveniently loaded into the top cover.

The firstrotatable body 3 penetrates through the first mounting hole, the firstrotatable body 3 is in clearance fit with the first mounting hole, the secondrotatable body 5 penetrates through the second mounting hole, the secondrotatable body 5 is in clearance fit with the second mounting hole, the thirdrotatable body 7 penetrates through the third mounting hole, the thirdrotatable body 7 is in clearance fit with the third mounting hole, the fourthrotatable body 9 penetrates through the fourth mounting hole, the fourthrotatable body 9 is in clearance fit with the fourth mounting hole, the fifthrotatable body 11 penetrates through the fifth mounting hole, the fifthrotatable body 11 is in clearance fit with the fifth mounting hole, and theclamp springs 23 are arranged at the small ends of the side walls of the firstrotatable body 3, the secondrotatable body 5, the thirdrotatable body 7, the fourthrotatable body 9 and the fifthrotatable body 11.

A firstimmiscible phase chamber 12 is provided in the firstrotatable body 3, a secondimmiscible phase chamber 13 is provided in the secondrotatable body 5, a thirdimmiscible phase chamber 14 is provided in the thirdrotatable body 7, a fourthimmiscible phase chamber 15 is provided in the fourthrotatable body 9, and adetection chamber 16 is provided in the fifthrotatable body 9. Thesample reagent chamber 2, thefirst purification chamber 4, and thesecond purification chamber 6 are all in a bucket shape, the firstimmiscible phase chamber 12, the secondimmiscible phase chamber 13, the thirdimmiscible phase chamber 14, thereaction chamber 8, the fourthimmiscible phase chamber 15, thethird purification chamber 10, and thedetection chamber 16 are all in a tubular shape, and the firstimmiscible phase chamber 12 and the secondimmiscible phase chamber 13 are all in a tapered shape. Thesample reagent cavity 2, the firstimmiscible phase cavity 12, thefirst purification cavity 4, the secondimmiscible phase cavity 13, thesecond purification cavity 6, the thirdimmiscible phase cavity 14, thereaction cavity 8, the fourthimmiscible phase cavity 15, thethird purification cavity 10 and thedetection cavity 16 are sequentially communicated, a small end outlet of thesample reagent cavity 2 is communicated with a large end inlet of the firstimmiscible phase cavity 12, a small end outlet of the firstimmiscible phase cavity 12 is communicated with a large end inlet of thefirst purification cavity 4, a small end outlet of thefirst purification cavity 4 is communicated with a large end inlet of the secondimmiscible phase cavity 13, a small end outlet of the secondimmiscible phase cavity 13 is communicated with a large end inlet of thesecond purification cavity 6, and a small end outlet of thesecond purification cavity 6 is communicated with an upper end port of the thirdimmiscible phase cavity 14.

The small end face of the firstrotatable body 3 is connected with a first connectingmale head 17, the small end face of the secondrotatable body 5 is connected with a second connectingmale head 18, the small end face of the thirdrotatable body 7 is connected with a third connectingmale head 19, the small end face of the fourthrotatable body 9 is connected with a fourth connectingmale head 20, the small end face of the fifthrotatable body 11 is connected with a fifth connectingmale head 21, and the first connectingmale head 17, the second connectingmale head 18, the third connectingmale head 19, the fourth connectingmale head 20 and the fifth connectingmale head 21 are all in a shape of a Chinese character 'yi'. The male connection head can be conveniently mechanically connected with the outside, thereby controlling the rotatable body to rotate.

A pair of firstsocket lug plates 22 is symmetrically arranged on the part of the side wall of thekit body 1 positioned on the periphery of the sample reagent cavity, a pair of second socket lug plates is symmetrically arranged on the part of the side wall of thekit body 1 positioned on the periphery of the first purification cavity, a pair of third socket lug plates is symmetrically arranged on the part of the side wall of thekit body 1 positioned on the periphery of the second purification cavity, a pair of fourth socket lug plates is symmetrically arranged on the part of the side wall of thekit body 1 positioned on the periphery of the reaction cavity, and a pair of fifth socket lug plates is symmetrically arranged on the part of the side wall of thekit body 1 positioned on the periphery of the third purification cavity. The firstsocket ear plate 22, the second socket ear plate, the third socket ear plate, the fourth socket ear plate and the fifth socket ear plate are in a straight line shape.

The pair of firstsocket lug plates 22, the first connectingmale head 17, the pair of second socket lug plates, the second connectingmale head 18, the pair of third socket lug plates, the third connectingmale head 19, the pair of fourth socket lug plates, the fourth connectingmale head 20, the pair of fifth socket lug plates and the fifth connectingmale head 21 are alternately distributed along a straight line. The plane where the first connecting male head, the second connecting male head, the third connecting male head, the fourth connecting male head and the fifth connecting male head are distributed is parallel to the first kit body.

The firstrotatable body 3, the secondrotatable body 5, the thirdrotatable body 7, the fourthrotatable body 9, and the fifthrotatable body 11 havegrip grooves 25 formed in the respective large end faces thereof in a straight shape, and a handle can be inserted into thegrip groove 25 to thereby rotate the firstrotatable body 3, the secondrotatable body 5, the thirdrotatable body 7, the fourthrotatable body 9, and the fifthrotatable body 11 under control.

The application method of the nucleic acid detection kit with the rotary valve core combination comprises the following steps:

1. adding a sample, a lysis solution and carboxylated magnetic beads into asample reagent cavity 2, adding a first purification solution into afirst purification cavity 4, adding a second purification solution into asecond purification cavity 6, adding a primer reaction solution into areaction cavity 8, adding an eluent into athird purification cavity 10, adding mineral oil into a firstimmiscible phase cavity 12, a secondimmiscible phase cavity 13, a thirdimmiscible phase cavity 14 and a fourthimmiscible phase cavity 15, and sealing by adding low-density mineral oil into adetection cavity 16 by utilizing the principle of oil-water incompatibility and the small sizes of the firstimmiscible phase cavity 12, the secondimmiscible phase cavity 13, the thirdimmiscible phase cavity 14 and the fourthimmiscible phase cavity 15, wherein the firstimmiscible phase cavity 12, the secondimmiscible phase cavity 13, the thirdimmiscible phase cavity 14 and the fourthimmiscible phase cavity 15 play a role in isolation;

2. adding a pretreated cell sample to be detected into asample reagent cavity 2, cracking the cell sample to be detected under the action of a lysis solution to release nucleic acid from cells, manually or electrically rotating a firstrotatable body 3 to a set position to isolate the firstrotatable body 3, then placing an ultrasonic device at the top of thesample reagent cavity 2, starting the ultrasonic device, crushing the cell sample to be detected under the action of ultrasonic vibration to further release the nucleic acid, closing the ultrasonic device after the release of the nucleic acid is finished, adsorbing the released nucleic acid by carboxyl functional groups attached to the surface of magnetic beads, and manually or externally and mechanically rotating the firstrotatable body 3 to the set position to reset the firstrotatable body 3 after the adsorption is finished;

3. the magnetic sucker is arranged on the side surface of thesample reagent cavity 2, the magnetic sucker is moved downwards through an external instrument or manually, magnetic beads with adsorbed nucleic acid move downwards under the attraction of the magnetic sucker, and when the magnetic beads with adsorbed nucleic acid pass through the firstimmiscible phase cavity 12, mineral oil in the firstimmiscible phase cavity 12 filters out impurities such as organelles, cell membranes or cell walls;

4. continuously moving the magnetic sucker downwards, continuously moving the magnetic beads adsorbed with the nucleic acid downwards under the attraction of the magnetic sucker, and when the magnetic beads adsorbed with the nucleic acid pass through thefirst purification cavity 4, carrying out first purification on the nucleic acid by using first purification liquid;

5. continuously moving the magnetic sucker downwards, continuously moving the magnetic beads with the adsorbed nucleic acids downwards under the attraction of the magnetic sucker, and filtering impurities dissolved in the oil phase by the mineral oil in the secondimmiscible phase cavity 13 when the magnetic beads with the adsorbed nucleic acids pass through the secondimmiscible phase cavity 13;

6. continuously moving the magnetic sucker downwards, continuously moving the magnetic beads adsorbed with the nucleic acid downwards under the attraction of the magnetic sucker, and when the magnetic beads adsorbed with the nucleic acid pass through thesecond purification cavity 6, performing secondary purification on the nucleic acid by using a second purification solution;

7. the magnetic sucker is continuously moved downwards, the magnetic beads with the adsorbed nucleic acids are continuously moved downwards under the attraction of the magnetic sucker, and when the magnetic beads with the adsorbed nucleic acids pass through the thirdimmiscible phase cavity 14, the mineral oil in the thirdimmiscible phase cavity 14 filters impurities dissolved in the oil phase;

8. the magnetic beads adsorbed with the nucleic acid continuously move downwards under the attraction of the magnetic attraction device, when the magnetic beads adsorbed with the nucleic acid pass through thereaction cavity 8, the primers in thereaction cavity 8 react with the nucleic acid, after the reaction is finished, the ultrasonic device is started, and reaction products fall off from the surfaces of the magnetic beads through the ultrasonic action;

9. the droplets with the reaction product are transferred from thereaction chamber 8 to the third purification chamber through the fourthimmiscible phase chamber 15 and then to thedetection chamber 16 under the action of the sonicator, and the sonicator is turned off;

10. heating the detection cavity by a heater to a preset temperature, wherein the upper part of thedetection cavity 16 is a high-temperature region, the lower part of thedetection cavity 16 is a low-temperature region, then, the detection cavity is driven by hand or external machinery to rotate for 360 degrees, and the dropping liquid moves between the high-temperature region and the low-temperature region rapidly under the action of self gravity, so that the rapid amplification of the nucleic acid is realized;

11. and carrying out nucleic acid detection after the nucleic acid amplification is finished.

Claims (9)

1. A nucleic acid detection kit with a rotary valve core assembly is characterized in that: the kit comprises a kit body, wherein the kit body is sequentially provided with a sample reagent cavity, a first rotatable body, a first purification cavity, a second rotatable body, a second purification cavity, a third rotatable body, a reaction cavity, a fourth rotatable body, a third purification cavity and a fifth rotatable body from top to bottom, the first rotatable body, the second rotatable body, the third rotatable body, the fourth rotatable body and the fifth rotatable body are respectively rotatably connected with the kit body, a first immiscible phase cavity is arranged in the first rotatable body, a second immiscible phase cavity is arranged in the second rotatable body, a third immiscible phase cavity is arranged in the third rotatable body, a fourth immiscible phase cavity is arranged in the fourth rotatable body, a detection cavity is arranged in the fifth rotatable body, and the sample reagent cavity, the first immiscible phase cavity, the first purification cavity, the second immiscible phase cavity, the second purification cavity, the third immiscible phase cavity, the reaction cavity, the fourth immiscible phase cavity, The third purification chamber and the detection chamber are communicated in sequence.

2. The nucleic acid detection kit with a rotary valve core assembly according to claim 1, wherein: the kit body is provided with a first mounting hole, a second mounting hole, a third mounting hole, a fourth mounting hole and a fifth mounting hole from top to bottom in sequence, wherein the first rotatable body is movably connected with the first mounting hole, the second rotatable body is movably connected with the second mounting hole, the third rotatable body is movably connected with the third mounting hole, the fourth rotatable body is movably connected with the fourth mounting hole, and the fifth rotatable body is movably connected with the fifth mounting hole.

3. The nucleic acid detection kit with a rotary valve core assembly according to claim 2, wherein: the first rotatable body, the second rotatable body, the third rotatable body, the fourth rotatable body and the fifth rotatable body are in a conical shape, the first rotatable body penetrates through the first mounting hole, the first rotatable body is in clearance fit with the first mounting hole, the second rotatable body penetrates through the second mounting hole, the second rotatable body is in clearance fit with the second mounting hole, the third rotatable body penetrates through the third mounting hole, the third rotatable body is in clearance fit with the third mounting hole, the fourth rotatable body penetrates through the fourth mounting hole, the fourth rotatable body is in clearance fit with the fourth mounting hole, the fifth rotatable body penetrates through the fifth mounting hole, and the fifth rotatable body is in clearance fit with the fifth mounting hole.

4. The nucleic acid detection kit with a rotary valve core assembly according to claim 2, wherein: the top of the kit body is provided with a top cover for covering the sample reagent cavity, and the top cover is clamped on the kit body.

5. The nucleic acid detection kit with a rotary valve core assembly according to claim 2, wherein: the small ends of the side walls of the first rotatable body, the second rotatable body, the third rotatable body, the fourth rotatable body and the fifth rotatable body are provided with clamp springs, the small end face of the fifth rotatable body is connected with a fifth connecting male head, and the large end faces of the first rotatable body, the second rotatable body, the third rotatable body, the fourth rotatable body and the fifth rotatable body are respectively provided with a straight handle groove.

6. The nucleic acid detection kit with the rotary valve core assembly according to claim 2, wherein: the small end face of the first rotatable body is connected with a first connecting male head, the small end face of the second rotatable body is connected with a second connecting male head, the small end face of the third rotatable body is connected with a third connecting male head, the small end face of the fourth rotatable body is connected with a fourth connecting male head, and the small end face of the fifth rotatable body is connected with a fifth connecting male head.

7. The nucleic acid detection kit with the rotary valve core assembly according to claim 6, wherein: the kit body is in a long and flat shape, a pair of first socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the sample reagent cavity, a pair of second socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the first purification cavity, a pair of third socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the second purification cavity, a pair of fourth socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the reaction cavity, a pair of fifth socket ear plates is symmetrically arranged on the part of the side wall of the kit body, which is positioned on the periphery of the third purification cavity, and the pair of first socket ear plates, the pair of second socket ear plates, the pair of third socket ear plates, the pair of fourth socket ear plates and the pair of fifth socket ear plates are distributed along a straight line;

the pair of first socket-joint ear plates, the first connecting male head, the pair of second socket-joint ear plates, the second connecting male head, the pair of third socket-joint ear plates, the third connecting male head, the pair of fourth socket-joint ear plates, the fourth connecting male head, the pair of fifth socket-joint ear plates and the fifth connecting male head are alternately distributed along a straight line.

8. The nucleic acid detection kit with a rotary valve core assembly according to claim 6, wherein: the first socket joint ear plate, the second socket joint ear plate, the third socket joint ear plate, the fourth socket joint ear plate, the fifth socket joint ear plate, the first connection male head, the second connection male head, the third connection male head, the fourth connection male head and the fifth connection male head are all in a shape like a Chinese character 'yi', and the plane where the first connection male head, the second connection male head, the third connection male head, the fourth connection male head and the fifth connection male head are distributed is parallel to the first kit body.

9. The nucleic acid detection kit with a rotary valve core assembly according to claim 2, wherein: the sample reagent cavity, the first purification cavity and the second purification cavity are all in a bucket shape, the first immiscible phase cavity, the second immiscible phase cavity, the third immiscible phase cavity, the reaction cavity, the fourth immiscible phase cavity, the third purification cavity and the detection cavity are all in a tubular shape, the first immiscible phase cavity and the second immiscible phase cavity are in a conical shape, a small end outlet of the sample reagent cavity is communicated with a large end inlet of the first immiscible phase cavity, a small end outlet of the first immiscible phase cavity is communicated with a large end inlet of the first purification cavity, a small end outlet of the first purification cavity is communicated with a large end inlet of the second immiscible phase cavity, a small end outlet of the second immiscible phase cavity is communicated with a large end inlet of the second purification cavity, and a small end outlet of the second purification cavity is communicated with an upper end port of the third immiscible phase cavity.

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