Poison seed suspension incubatorTechnical Field
The invention relates to the technical field of virus seed culture, in particular to a virus seed suspension incubator.
Background
The virus needs to be cultured when the virus is subjected to experiments, the virus seeds are cultured under the laboratory conditions and are protoplasts of the virus, the suspension culture method is mostly adopted for culturing the virus seeds in the culture process, the suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is constantly stirred or shaken, and is a culture mode of anchorage-independent cells, certain anchorage-dependent cells can also be cultured by the method after adaptation and selection, the increase of the suspension culture scale is relatively simple, the depth is more than 5mm, the culture medium needs to be stirred and is more than 10cm, and CO2 and air need to be deeply introduced to ensure sufficient gas exchange. The cells are always in a dispersed suspension in the culture solution by shaking or rotating the device.
Most of the existing virus seed suspension culture methods adopt stirring and air floatation methods to enable cells hosted by virus seeds to be suspended in a culture solution, the suspension persistence of the methods is insufficient, and the stable culture environment of the virus seeds can be damaged during stirring and air floatation operations, so that the survival rate of the virus seed culture is influenced, and therefore, a virus seed suspension culture box is provided.
Disclosure of Invention
The invention aims to provide a virus seed suspension culture box, which aims to solve the problems that most of the conventional virus seed suspension culture methods proposed in the background art adopt stirring and air floatation methods to suspend cells hosted by virus seeds in a culture solution, the suspension persistence of the methods is insufficient, and the stable culture environment of the virus seeds is damaged during stirring and air floatation operations.
In order to achieve the purpose, the invention provides the following technical scheme: a suspension incubator for seeds of poison comprises a push rod control cabinet, wherein a horizontal platform seat is arranged at the lower end of the push rod control cabinet, two electric push rods are arranged at the upper end of the horizontal platform seat, an incubator is arranged at the upper end of the push rod control cabinet, an inner culture frame is arranged in the incubator, an intermediate culture tank is arranged in the inner culture frame, an intermediate tank support seat is arranged in the intermediate culture tank, an intermediate culture tank placing tank is arranged in the intermediate tank support seat, a transparent culture tank is arranged at the upper end of the intermediate culture tank placing tank, a charged disc is arranged in the transparent culture tank, a seed culture solution is arranged above the charged disc, the seed culture solution is a mixed aqueous solution of pure water, amino acid, monosaccharide, vitamin, inorganic ions and trace elements, and a rear connecting plate is arranged on the rear end face of the intermediate culture tank, the front end face of the rear connecting plate is provided with a wireless network camera, and the front end face of the incubator is provided with a movable door sealing plate.
Preferably, be provided with the sealing groove on the position that dodge gate shrouding and incubator are connected, the sealing groove sets up with incubator integrated into one piece, all be provided with the direction logical groove on the both sides inner wall in sealing groove, the direction leads to the groove and sets up with sealing groove integrated into one piece, the inside that the direction led to the groove is provided with the transmission armed lever, the both ends of transmission armed lever respectively with dodge gate shrouding and electric putter's output welded connection.
Preferably, the inner walls of the two sides of the guide through groove are provided with through groove inner walls, the through groove inner walls and the guide through groove are integrally formed, the outer walls of the two sides of the transmission arm rod are provided with arm rod inner grooves, the arm rod inner grooves are internally provided with auxiliary rollers, and the transmission arm rod is in rolling connection with the through groove inner walls through the auxiliary rollers.
Preferably, the inner walls of the two sides of the arm lever inner groove are provided with bearings, the bearings are fixedly connected with the arm lever inner groove through screws, the outer walls of the two sides of the auxiliary roller are provided with connecting shafts, the connecting shafts and the auxiliary roller are integrally formed, and the auxiliary roller is movably connected with the bearings through the connecting shafts on the outer walls of the two sides.
Preferably, be provided with the internal frame back plate on the rear end position of training the internal frame, be provided with four corresponding connecting rods on the rear end inner wall of incubator, the both ends that correspond the connecting rod respectively with incubator and internal frame back plate welded connection, the top of incubator is provided with the power supply cabinet, the inside of internal frame back plate is provided with twelve winding displacement perforations, and the winding displacement perforation sets up with internal frame back plate integrated into one piece, twelve winding displacement perforations respectively with twelve cultivate the intervallum groove one-to-one, the electricity of wireless network camera is served and is provided with the connecting wire.
Preferably, four be provided with integrated row of inserting on the position between the corresponding connecting rod, integrated row of inserting passes through screw and incubator fixed connection, twelve connect the electric wire and run through the winding displacement perforation and with integrated row electric connection that inserts, integrated row of inserting connects to be provided with the power supply wiring on holding, integrated row of inserting passes through power supply wiring and power supply cabinet electric connection.
Preferably, the incubator is made of stainless steel, the thickness of the stainless steel is twenty centimeters, and the inner wall of the stainless steel is lined with ten centimeters of polyurethane foam.
Preferably, a manual operator is arranged on the front end face of the push rod control cabinet, the output end of the manual operator is electrically connected with the input end of the push rod control cabinet, and the output end of the push rod control cabinet is electrically connected with the input ends of the two electric push rods.
Preferably, be provided with wireless AP, router and network video recorder on the rear end outer wall of incubator, be provided with supporting platform on one side outer wall of incubator, supporting platform and incubator integrated into one piece set up, supporting platform's upper end is provided with the display, wireless network camera's output and wireless AP's input transmission connection.
Preferably, the output end of the wireless AP is electrically connected with the input end of the router, the output end of the router is electrically connected with the input end of the network video recorder, and the output end of the network video recorder is electrically connected with the input end of the display.
Compared with the prior art, the invention has the beneficial effects that:
1. the suspension culture box of the invention adopts the principle of mutual exclusion of same level of charges to realize the effect of suspension culture of the virus seeds without stirring and floating operation, the main body of the virus seed culture solution is pure water which is not conductive, a disc with charges of same level is arranged below the virus seed culture solution, the virus seeds are lodged and cultured in cells which are positioned inside the virus seed culture solution, according to the principle of mutual exclusion of the same level of charges, the cells in the virus seed culture solution are suspended in the virus seed culture solution under the influence of charge repulsion, because the pure water is not conductive, the suspension process can not affect the culture environment of the virus seeds, overcomes the defect that the prior suspension culture of the virus seeds mostly adopts stirring and air flotation modes to suspend the cells hosted by the virus seeds in the culture solution, these methods have problems that the suspension is not sufficiently durable and the stable culture environment of the seed is damaged during the agitation and air flotation operations.
2. The cultivation is not finished, the box does not need to be opened, the virus seed suspension cultivation condition in each cultivation chamber can be monitored in real time through the wireless network cameras, each cultivation chamber is provided with an independent wireless network camera, twelve wireless network cameras receive the power supply of the top power supply cabinet, the wireless network cameras upload the images monitored in real time to the display through the wireless AP, the router and the network hard disk video recorder to display, and therefore the virus seed suspension cultivation condition can be mastered in real time under the condition that the box does not need to be opened.
3. The chamber door of incubator is connected by hinge formula among the prior art and is changed into the gate-type, has improved closed sealed degree, has reduced the influence that external factor caused incubator internal environment, after the cultivation cycle, accessible presses two electric putter synchronous jack-ups of button control on the manual operator when needing to use the poison seed to open the dodge gate shrouding, be convenient for take out the poison seed of cultivation.
Drawings
FIG. 1 is a schematic view of the overall structure of the suspension incubator for virus seeds according to the present invention;
FIG. 2 is a schematic view of the overall closed structure of the suspension incubator for virus seeds according to the present invention;
FIG. 3 is a schematic view of the internal structure of a culture chamber of the present invention;
FIG. 4 is a schematic view of the construction of a transparent culture tank of the present invention;
FIG. 5 is a schematic diagram of the construction of the rear panel of the inner frame of the present invention;
FIG. 6 is a schematic view of a connecting structure of a driving arm lever and a guiding through groove of the present invention;
FIG. 7 is a schematic view of the wireless network camera working process of the present invention;
in the figure: 1. the push rod controls the chassis; 2. a horizontal platform base; 3. a manual operator; 4. an electric push rod; 5. an incubator; 6. culturing an inner frame; 7. a movable door closing plate; 8. sealing the connecting groove; 9. a guide through groove; 10. a drive arm; 11. a support platform; 12. a display; 13. a culture compartment; 14. a middle groove supporting seat; 15. a transparent culture tank; 16. a power supply cabinet; 17. a rear connecting plate; 18. a culture tank placing groove; 19. a wireless network camera; 20. connecting a wire; 21. a charged disc; 22. a seed culture solution; 23. an inner frame rear plate; 24. a corresponding connecting rod; 25. perforating the flat cable; 26. an integrated power strip; 27. a power supply wiring; 28. the inner wall of the through groove is provided with a groove; 29. an arm lever inner groove; 30. an auxiliary roller; 31. a bearing; 32. a connecting shaft; 33. a wireless AP; 34. a router; 35. network hard disk video recorder.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-7, an embodiment of the present invention is shown: a suspension incubator for virus seeds comprises a push rod control case 1, a horizontal platform base 2 is arranged at the lower end of the push rod control case 1, two electric push rods 4 are arranged at the upper end of the horizontal platform base 2, an incubator 5 is arranged at the upper end of the push rod control case 1, an inner culture frame 6 is arranged inside the incubator 5, an incubation chamber groove 13 is arranged inside the inner culture frame 6, an chamber groove supporting base 14 is arranged inside the incubation chamber groove 13, an incubator placing groove 18 is arranged inside the chamber groove supporting base 14, a transparent incubator 15 is arranged at the upper end of the incubator placing groove 18, a disc 21 with charges is arranged inside the transparent incubator 15, a virus seed culture solution 22 is arranged above the disc 21 with charges, the virus seed culture solution 22 is a mixed aqueous solution of pure water, amino acid, monosaccharide, vitamin, inorganic ions and trace elements, a post-positioned connecting plate 17 is arranged on the rear end face of the incubation chamber groove 13, the front end face of the rear connecting plate 17 is provided with a wireless network camera 19, and the front end face of the incubator 5 is provided with a movable door closing plate 7.
Further, be provided with sealingconnection groove 8 on the position that dodge gate shrouding 7 andincubator 5 are connected, sealingconnection groove 8 sets up withincubator 5 integrated into one piece, all be provided with thelogical groove 9 of direction on sealingconnection groove 8's the both sides inner wall, thelogical groove 9 of direction sets up with sealingconnection groove 8 integrated into one piece, the inside that leads to groove 9 is provided with transmissionarmed lever 10, the both ends of transmissionarmed lever 10 respectively with dodge gate shrouding 7 and electric putter 4's output welded connection, sealingconnection groove 8 that sets up on the position that dodge gate shrouding 7 andincubator 5 are connected plays the effect of being convenient for dodge gate shrouding 7 andincubator 5 activity sealing connection, thelogical groove 9 of direction that all sets up on the both sides inner wall of sealingconnection groove 8 plays the effect of being convenient for transmissionarmed lever 10 direction activity, transmissionarmed lever 10 that the inside that leads to groove 9 set up plays the effect of transmission electric putter 4 and dodge gate shrouding 7.
Further, the inner walls of the two sides of the guide throughgroove 9 are provided with through grooveinner wall grooves 28, the through grooveinner wall grooves 28 and the guide throughgroove 9 are formed integrally, the outer walls of the two sides of thedrive arm 10 are provided with arm rodinner grooves 29, the inside of the arm rodinner grooves 29 is provided withauxiliary rollers 30, thedrive arm 10 is in rolling connection with the through grooveinner wall grooves 28 through theauxiliary rollers 30, the through grooveinner wall grooves 28 arranged on the inner walls of the two sides of the guide throughgroove 9 play a role in facilitating the rolling contact connection of theauxiliary rollers 30 and the guide throughgroove 9, and the arm rodinner grooves 29 arranged on the outer walls of the two sides of thedrive arm 10 play a role in bearing theauxiliary rollers 30.
Further,bearings 31 are arranged on inner walls of two sides of the arm leverinner groove 29, thebearings 31 are fixedly connected with the arm leverinner groove 29 through screws, connectingshafts 32 are arranged on outer walls of two sides of theauxiliary roller 30, the connectingshafts 32 and theauxiliary roller 30 are integrally formed, theauxiliary roller 30 is movably connected with thebearings 31 through the connectingshafts 32 on the outer walls of the two sides, thebearings 31 arranged on the inner walls of two sides of the arm leverinner groove 29 play a role in movably connecting the auxiliary connectingshafts 32, and the connectingshafts 32 arranged on the outer walls of two sides of theauxiliary roller 30 play a role in facilitating connection of theauxiliary roller 30 and thebearings 31.
Furthermore, an inner framerear plate 23 is arranged at the rear end of the cultureinner frame 6, four corresponding connectingrods 24 are arranged on the inner wall of the rear end of theincubator 5, the both ends that correspond connectingrod 24 respectively withincubator 5 and internal frame backplate 23 welded connection, the top ofincubator 5 is provided withpower supply cabinet 16, the inside of internal frame backplate 23 is provided with twelve windingdisplacement perforation 25, windingdisplacement perforation 25 sets up with internal frame backplate 23 integrated into one piece, twelve windingdisplacement perforation 25 respectively with twelve cultivate betweengroove 13 one-to-one, be provided withpower connection wire 20 onwireless network camera 19's the power connection end, cultivate the internal frame backplate 23 that sets up on the rear end position ofinternal frame 6 and play the effect of bearing twelve windingdisplacement perforation 25,power supply cabinet 16 that the top ofincubator 5 set up plays the effect forwireless network camera 19 power supply, the twelve windingdisplacement perforation 25 that the inside of internal frame backplate 23 set up play the effect that is convenient forpower connection wire 20 to run through and arranges.
Furthermore, four positions between the corresponding connectingrods 24 are provided with integrated socket bars 26, the integrated socket bars 26 are fixedly connected with theincubator 5 through screws, twelveelectric wires 20 penetrate through the flat cable throughholes 25 and are electrically connected with the integrated socket bars 26, the electric connection ends of the integrated socket bars 26 are provided with powersupply connection wires 27, the integrated socket bars 26 are electrically connected with thepower supply cabinet 16 through the powersupply connection wires 27, the integrated socket bars 26 arranged at the positions between the four corresponding connectingrods 24 play a role in conveniently and uniformly connecting the twelveelectric wires 20, and the powersupply connection wires 27 arranged at the electric connection ends of the integrated socket bars 26 play a role in conveniently connecting the integrated socket bars 26 with thepower supply cabinet 16 for power supply.
Furthermore, theincubator 5 is made of stainless steel, the thickness of the stainless steel is twenty centimeters, the inner wall of the stainless steel is lined with ten centimeters of polyurethane foam, the heat insulation material of the incubator body is made of polyurethane foam, the incubator body has strong anti-interference capability on external cold and heat sources, and the incubator body is made of stainless steel and has good corrosion resistance.
Further, amanual operator 3 is arranged on the front end face of the push rod control case 1, the output end of themanual operator 3 is electrically connected with the input end of the push rod control case 1, the output end of the push rod control case 1 is electrically connected with the input ends of the two electric push rods 4, and themanual operator 3 arranged on the front end face of the push rod control case 1 plays a role of sending an instruction to the push rod control case 1, so that the purpose of controlling and adjusting the electric push rods 4 is achieved.
Further, be provided with wireless AP33 on the rear end outer wall ofincubator 5,router 34 and network harddisk video recorder 35, be provided with supportingplatform 11 on one side outer wall ofincubator 5, supportingplatform 11 sets up withincubator 5 integrated into one piece, supportingplatform 11's upper end is provided withdisplay 12,wireless network camera 19's output andwireless AP 33's input transmission connection, the wireless AP33 that sets up on the rear end outer wall ofincubator 5,router 34 and network harddisk video recorder 35 play the effect of constituteing wireless image signal transmission system.
Further, the output end of the wireless AP33 is electrically connected to the input end of therouter 34, the output end of therouter 34 is electrically connected to the input end of the networkhard disk recorder 35, and the output end of the networkhard disk recorder 35 is electrically connected to the input end of thedisplay 12.
The working principle is as follows: the suspension culture box adopts the principle of mutual exclusion of the same level of charges to realize the effect of suspension culture of the virus seeds without stirring and floating operation, firstly selects proper boarder cells according to the types of the virus seeds to be cultured, prepares a disc 21 with charges the same as the selected boarder cells according to the charges carried by the selected boarder cells, for example, the surface of a normal erythrocyte is negatively charged, the surface of a myocardial cell in a stationary period is positively charged, so the charges carried by the prepared disc 21 are determined by the charges carried by the cells to be cultured by the virus seeds, the preparation of the disc 21 adopts the reason of electrostatic induction, puts the disc 21 with charges into an electrostatic field, obtains the positive charges carried from a negative pole, obtains the negative charges carried from a positive pole, the main body of the virus seed culture solution 22 is pure water, the pure water is not conductive, the disc 21 with charges of the same level is put below the virus seed culture solution 22, the virus seeds are hosted and cultured in the cells, the cells are positioned in the virus seed culture solution 22, according to the principle of the mutual exclusion of the same level of charges, the cells in the virus seed culture solution are influenced by the mutual repulsion of the charges and are suspended in the virus seed culture solution, the suspension process cannot influence the virus seed culture environment because pure water is not conductive, the door of the incubator 5 is replaced by a shutter type door from hinge type connection in the prior art, the closed sealing degree is improved, the influence of external factors on the internal environment of the incubator 5 is reduced, the incubator does not need to be opened before the culture is not finished, the suspension culture condition of the virus seeds in each culture compartment groove 13 can be monitored in real time through the wireless network cameras 19, each culture compartment groove 13 is provided with an independent wireless network camera 19, twelve wireless network cameras 19 receive the power supply of the top power supply cabinet 16, and the wireless network cameras 19 monitor the suspension culture condition of the virus seeds in each culture compartment groove 13 through the wireless AP33, Router 34 and network video recorder 35 upload real time monitoring's image to display 12 on showing to also can master the circumstances that the suspension of poison kind was cultivateed in real time under the circumstances that need not to unpack, after the cultivation cycle, the accessible presses two electric putter 4 synchronous jack-ups of button control on the manual operator 3 when needing to use the poison kind, thereby open dodge gate shrouding 7, be convenient for take out the poison kind of cultivation, accomplish the use work of poison kind suspension incubator.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.