


技术领域technical field
本发明涉及微流控系统监测领域,具体涉及原位监测微通道中流体状态的新型光纤光栅探头The invention relates to the field of microfluidic system monitoring, in particular to a novel fiber grating probe for in-situ monitoring of the fluid state in a microchannel
背景技术Background technique
微流控芯片是当前微全分析系统发展的热点领域,它是将整个化验室的功能,包括采样、稀释、加试剂、反应、分离、检测等集成在微芯片上,且可以多次使用,其优点十分显著,已经发展成为一个生物、化学、医学、流体、电子、材料、机械等学科交叉的崭新研究领域。近些年来,微流控制技术一直在快速发展,并在化学合成,环境检测,药物开发,生物分析和光学技术应用等方面得到了广泛的应用。在这些实验室芯片应用中,流体的流速、流态起着重要的影响作用,流体的流速主导着反应产生及发生的效果。Microfluidic chip is a hot spot in the development of the current micro-total analysis system. It integrates the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc. on the microchip, and can be used multiple times. Its advantages are very significant, and it has developed into a new research field interdisciplinary in biology, chemistry, medicine, fluids, electronics, materials, and machinery. In recent years, microfluidic control technology has been developing rapidly and has been widely used in chemical synthesis, environmental detection, drug development, bioanalysis and optical technology applications. In these lab-on-a-chip applications, the flow rate and flow state of the fluid play an important role, and the flow rate of the fluid dominates the generation and effect of the reaction.
光栅光纤具有体积小、熔接损耗小、全兼容于光纤、能埋入智能材料等优点,其谐振波长对温度、应变、折射率、浓度等外界环境的变化比较敏感,因此在光纤传感器领域(位移、速度、加速度、温度的测量)应用广泛。但是,由于其本身较为脆弱,在微流控监测中受到了极大的限制。因此,如何实现光纤光栅的封装以适应各种微通道环境,变得尤为重要。Grating fiber has the advantages of small size, low splicing loss, full compatibility with optical fiber, and can be embedded in smart materials. Its resonant wavelength is sensitive to changes in external environment such as temperature, strain, refractive index, concentration, etc. , speed, acceleration, temperature measurement) are widely used. However, due to its fragility, it is greatly limited in microfluidic monitoring. Therefore, how to realize the encapsulation of fiber gratings to adapt to various microchannel environments becomes particularly important.
发明内容SUMMARY OF THE INVENTION
鉴于现有技术中的上述缺陷或不足,期望提供原位监测微通道中流体状态的新型光纤光栅探头。In view of the above-mentioned defects or deficiencies in the prior art, it is desirable to provide a novel fiber grating probe for monitoring the fluid state in a microchannel in situ.
根据本申请实例提供的技术方案,原位监测微通道中流体状态的新型光纤光栅探头包括一号LC接头、二号LC接头、一号PVC法兰、三号LC接头、中空连接管、四号LC接头、二号PVC法兰、五号LC接头、中空探头管、鲁尔口接口、盖帽、光栅、针头、鲁尔口、微流控芯片中的微通道;According to the technical solutions provided by the examples of this application, the novel fiber grating probe for in-situ monitoring of the fluid state in the microchannel includes No. 1 LC joint, No. 2 LC joint, No. 1 PVC flange, No. 3 LC joint, hollow connecting pipe, No. 4 LC joint LC connector, No. 2 PVC flange, No. 5 LC connector, hollow probe tube, Luer port, cap, grating, needle, Luer port, microchannel in microfluidic chip;
一号LC接头通过跳线与二号LC接头一端连接,二号LC接头的另一端通过螺纹与一号PVC法兰一端连接,一号PVC法兰的另一端通过螺纹用三号LC接头一端连接,三号LC接头的另一端通过AB胶与中空连接管连接,中空连接管的另一端与四号LC接头连接,四号LC接头的另一端通过螺纹与二号PVC法兰连接,二号PVC法兰的另一端通过螺纹与五号LC接头连接,五号LC接头的跳线依次穿过中空探头管和针头与光栅连接,光栅上装有盖帽,对光栅进行保护,光栅和针头作为插入鲁尔口部分与微流控芯片中的微通道相连,鲁尔口通过螺纹与鲁尔口接口连接。The No. 1 LC connector is connected to one end of the No. 2 LC connector through a jumper, the other end of the No. 2 LC connector is connected to one end of the No. 1 PVC flange through a thread, and the other end of the No. 1 PVC flange is connected to one end of the No. 3 LC connector through a thread , the other end of the No. 3 LC joint is connected with the hollow connecting pipe through AB glue, the other end of the hollow connecting pipe is connected with the No. 4 LC joint, the other end of the No. 4 LC joint is connected with the No. 2 PVC flange through threads, and the No. 2 PVC The other end of the flange is connected with the No. 5 LC connector through threads. The jumper of the No. 5 LC connector is connected to the grating through the hollow probe tube and the needle in turn. The grating is equipped with a cap to protect the grating. The grating and the needle are inserted as Luer The port part is connected with the microchannel in the microfluidic chip, and the luer port is connected with the luer port interface through a thread.
跳线是一种采用塑料皮层包裹裸光纤的保护皮套。A patch cord is a protective holster that wraps a bare optical fiber with a plastic sheath.
其中,三号LC接头连接的跳线穿过中空连接管与四号LC接头的跳线通过光栅熔接机进行熔接,五号LC接头的跳线与光栅之间通过光栅熔接机进行熔接。Among them, the jumper connected by No. 3 LC joint passes through the hollow connecting tube and the jumper of No. 4 LC joint is spliced by a grating fusion splicer, and the jumper of No. 5 LC joint and the grating are welded by a grating fusion splicer.
鲁尔口接口表面有螺纹,与鲁尔口相匹配,通过螺纹调整插入的深度,进而检测不同尺寸、不同深度的微通道流体状态。The surface of the luer port is threaded, which matches the luer port, and the depth of insertion is adjusted through the thread, thereby detecting the fluid state of microchannels of different sizes and depths.
鲁尔口以插入的形式与微流控芯片中的微通道连接,其中针头和光栅为插入部分,根据测试区域调整插入部分,可测得微通道边界流速、流态,也可根据需要测试中心处的流动情况。The luer port is connected to the microchannel in the microfluidic chip in the form of insertion, in which the needle and the grating are the insertion part, and the insertion part is adjusted according to the test area. flow situation.
光栅探头可以根据需要插入芯片中的不同位置,在芯片制作过程中预制作合适的鲁尔口进行插入连接即可。The grating probe can be inserted into different positions in the chip as required, and a suitable Luer port can be prefabricated for insertion and connection during the chip fabrication process.
通过光纤光栅可以选取包层模共振区,当液体流过光纤探头时,通过光谱仪中检测透射光谱,由波长的漂移量和漂移方向判断微流体的速度和方向,通过选择适当的光谱仪扫描频率,在一个扫描周期内观察沿着传感器移动的液体的整个过程,获得微流液体的流速、流动方向及流态。The cladding mode resonance region can be selected by the fiber grating. When the liquid flows through the fiber probe, the transmission spectrum is detected in the spectrometer, and the speed and direction of the microfluid are judged by the amount of wavelength drift and the drift direction. By selecting the appropriate spectrometer scanning frequency, Observe the entire process of the liquid moving along the sensor in one scanning cycle, and obtain the flow rate, flow direction and flow pattern of the microfluidic liquid.
综上所述,本申请的有益效果:To sum up, the beneficial effects of the present application:
(1)本发明的装配设计增加了光纤光栅在微流控通道中水力学监测过程的可操作性。(1) The assembly design of the present invention increases the operability of the fiber grating in the hydraulic monitoring process in the microfluidic channel.
(2)本发明可定制不同长度不同材质的光纤及鲁尔口,适用于不同尺寸的微通道测试。(2) The present invention can customize optical fibers and luer ports of different lengths and materials, and is suitable for microchannel testing of different sizes.
(3)本发明可随时随地进行组装,简便易行。(3) The present invention can be assembled anytime and anywhere, and is simple and easy to implement.
附图说明Description of drawings
通过阅读参照以下附图所作的对非限制性实施例所作的详细描述,本申请的其它特征、目的和优点将会变得更明显:Other features, objects and advantages of the present application will become more apparent by reading the detailed description of non-limiting embodiments made with reference to the following drawings:
图1为本发明的装配方案示意图;Fig. 1 is the assembly scheme schematic diagram of the present invention;
图2为本发明连接部位的示意图;Fig. 2 is the schematic diagram of the connection part of the present invention;
图3为本发明的部分结构示意图。FIG. 3 is a schematic diagram of a part of the structure of the present invention.
图中标号:一号LC接头-1;二号LC接头-2;一号PVC法兰-3;三号LC接头-4;中空连接管-5;四号LC接头-6;二号PVC法兰-7;五号LC接头-8;中空探头管-9;鲁尔口接口-10;盖帽-11;光栅-12;针头-13;鲁尔口-14;微流控芯片中的微通道-15。Labels in the figure: No. 1 LC joint-1; No. 2 LC joint-2; No. 1 PVC flange-3; No. 3 LC joint-4; Hollow connecting pipe-5; No. 4 LC joint-6; No. 2 PVC method Lan-7; No. 5 LC Connector-8; Hollow Probe Tube-9; Luer Port-10; Cap-11; Grating-12; Needle-13; Luer Port-14; Microchannel in Microfluidic Chip -15.
具体实施方式Detailed ways
下面结合附图和实施例对本申请作进一步的详细说明。可以理解的是,此处所描述的具体实施例仅仅用于解释相关发明,而非对该发明的限定。另外还需要说明的是,为了便于描述,附图中仅示出了与发明相关的部分。The present application will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the related invention, but not to limit the invention. In addition, it should be noted that, for the convenience of description, only the parts related to the invention are shown in the drawings.
需要说明的是,在不冲突的情况下,本申请中的实施例及实施例中的特征可以相互组合。下面将参考附图并结合实施例来详细说明本申请。如图1和图2所示,本发明用于原位监控微通道中流体状态的新型光纤光栅探头及与微流控连接部位的示意图,包括依次连接的一号LC接头1、二号LC接头2、一号PVC法兰3、三号LC接头4、中空连接管5、四号LC接头6、二号PVC法兰7、五号LC接头8、中空探头管9、鲁尔口接口10、盖帽11;光栅12;针头13;鲁尔口14;微通道15。It should be noted that the embodiments in the present application and the features of the embodiments may be combined with each other in the case of no conflict. The present application will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments. As shown in Figures 1 and 2, the present invention is a schematic diagram of a novel fiber grating probe used for in-situ monitoring of the fluid state in the microchannel and a schematic diagram of the connection part with the microfluidic control, including the No. 1 LC joint 1 and No. 2 LC joint connected in sequence 2. No. 1
一号LC接头1和二号LC接头2通过跳线连接,二号LC接头2和一号PVC法兰3通过螺纹连接,一号PCV法兰3和三号LC接头4通过螺纹连接,三号LC接头4和中空连接管5通过AB胶连接,中空连接管5和四号LC接头6通过AB胶连接,四号LC接头6和二号PVC法兰7通过螺纹连接,二号PVC法兰7和五号LC接头8通过螺纹连接,五号LC接头8和中空探头管9通过AB胶连接。No. 1 LC joint 1 and No. 2 LC joint 2 are connected by jumpers, No. 2 LC joint 2 and No. 1
其中,所述跳线是一种采用塑料皮层包裹裸光纤的保护皮套。所述三号LC接头4连接的跳线穿过中空连接管5与四号LC接头6连接。所述五号LC接头8连接的跳线依次穿过中空探头管9、针头13,且跳线的端部连接光栅12,其中,当五号LC接头8连接的跳线穿过中空探头管9后,需要采用AB胶对跳线进行固定,针头13一端插入中空探头管9约2mm,并采用AB胶进行粘接固定,所述光栅12伸出针头13一定长度,针头13与光栅12无需额外固定,采用盖帽11对光栅12进行保护,光栅12和针头13作为插入鲁尔口14部分与微通道15相连,鲁尔口14通过螺纹与鲁尔口接口10连接。Wherein, the jumper is a protective holster that uses a plastic skin to wrap the bare optical fiber. The jumper wire connected to the No. 3 LC joint 4 is connected to the No. 4 LC joint 6 through the hollow connecting
三号LC接头4和四号LC接头6之间的跳线通过光栅熔接机进行熔接,具体地,先将三号LC接头4的跳线一端深入中空连接管5直至达到另一端,然后经过光栅熔接机将跳线内的裸光纤与四号LC接头6端的跳线内的裸光纤进行熔接,熔接后多余的跳线放入中空连接管5内。五号LC探头8端的跳线与光栅11通过光栅熔接机熔接,进而插入中空探头管9,直至穿过针头13,伸出长度可(此为栅区长度)根据具体栅区长度可进行调节,此处为2mm。The jumper wire between No. 3 LC joint 4 and No. 4 LC joint 6 is welded by a grating fusion splicer. Specifically, first, one end of the jumper wire of No. 3 LC joint 4 is penetrated into the hollow connecting
中空连接管5和四号LC接头6通过AB胶连接,具体地,通过将四号LC接头6一端跳线插入中空连接管5,然后将AB胶注入中空连接管5头部凹槽内,进一步将四号LC接头6与凹槽对接,进而达到封装的目的。The hollow connecting
鲁尔口接口10表面有螺纹,与鲁尔口14相匹配,通过螺纹调整插入的深度,进而检测不同尺寸、不同深度的微通道流体状态。The surface of the
鲁尔口14以插入的形式与微流控芯片中的微通道15连接,其中针头13和光栅12为插入部分,根据测试区域调整插入部分,可测得微通道边界流速、流态,也可根据需要测试中心处的流动情况。The
所述五号LC接头8、中空探头管9、针头13、光栅12和盖帽11组成探头区域,该区域可实现光栅角度的变化。The No. 5
实施例1Example 1
本方案原位监测微通道中流体状态的新型光纤光栅探头中,一号LC接头1与光纤光栅解调仪连接,一号LC接头1与二号LC接头之间的跳线长度可以根据实际应用定制长度,此处长度为5cm。中空连接管5的长度和直径根据实际应用可以定制,此处为了减小其对监测过程中水力学的影响,采用两端直径为6mm、中间部位直径为2mm的中空管,长度为20mm和50mm两种规格。中空探头管9直径为6mm,长度为20mm。针头13总长度为5mm,针管长为4mm,外径0.05mm,内径0.025mm,针头13可根据实际情况进行调节。In this new type of fiber grating probe for in-situ monitoring of the fluid state in the microchannel, the No. 1 LC connector 1 is connected to the fiber grating demodulator, and the length of the jumper between the No. 1 LC connector 1 and the No. 2 LC connector can be based on the actual application. Customized length, here the length is 5cm. The length and diameter of the hollow connecting
具体实施过程为将一号LC接头1连接到光纤光栅解调仪,然后再将信号进一步反馈至电脑上,通过配套软件可实现受力变化的直接呈现,在没有收到外届力的干扰时,信号处于稳定阶段,当收到外界力干扰后,信号出现明显的变化。通过解调仪发出的光在光栅敏感部位折射的变化,来计算剪切力、进行流速流态的确定。The specific implementation process is to connect the No. 1 LC connector 1 to the fiber grating demodulator, and then further feedback the signal to the computer. Through the supporting software, the force change can be directly displayed. When there is no external force interference , the signal is in a stable stage, and the signal changes significantly after receiving external force interference. Through the change of refraction of light emitted by the demodulator at the sensitive part of the grating, the shear force is calculated and the flow rate and flow state are determined.
以上描述仅为本申请的较佳实施例以及对所运用技术原理等方案的说明。同时,本申请中所涉及的发明范围,并不限于上述技术特征的特定组合而成的技术方案,同时也应涵盖在不脱离所述发明构思的情况下,由上述技术特征或其等同特征进行任意组合而形成的其它技术方案。例如上述特征与本申请中公开的(但不限于)具有类似功能的技术特征进行互相替换而形成的技术方案。The above description is only a preferred embodiment of the present application and an illustration of the applied technical principles and other solutions. At the same time, the scope of the invention involved in this application is not limited to the technical solution formed by the specific combination of the above-mentioned technical features, and should also include the above-mentioned technical features or their equivalents without departing from the concept of the invention. Other technical solutions formed by any combination. For example, a technical solution is formed by replacing the above-mentioned features with the technical features disclosed in this application (but not limited to) with similar functions.
| Application Number | Priority Date | Filing Date | Title | 
|---|---|---|---|
| CN202210200708.6ACN114527296A (en) | 2022-03-02 | 2022-03-02 | Novel fiber grating probe for in-situ monitoring of fluid state in microchannel | 
| Application Number | Priority Date | Filing Date | Title | 
|---|---|---|---|
| CN202210200708.6ACN114527296A (en) | 2022-03-02 | 2022-03-02 | Novel fiber grating probe for in-situ monitoring of fluid state in microchannel | 
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| CN114527296Atrue CN114527296A (en) | 2022-05-24 | 
| Application Number | Title | Priority Date | Filing Date | 
|---|---|---|---|
| CN202210200708.6APendingCN114527296A (en) | 2022-03-02 | 2022-03-02 | Novel fiber grating probe for in-situ monitoring of fluid state in microchannel | 
| Country | Link | 
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| CN (1) | CN114527296A (en) | 
| Publication number | Priority date | Publication date | Assignee | Title | 
|---|---|---|---|---|
| CN101995485A (en)* | 2010-11-03 | 2011-03-30 | 山东大学 | Target fiber grating rheometer | 
| CN103512593A (en)* | 2012-06-20 | 2014-01-15 | 山东金煜电子科技有限公司 | Embedded fiber grating sensor and manufacturing method | 
| CN110836853A (en)* | 2019-12-03 | 2020-02-25 | 河南渡盈光电科技有限公司 | Microfluidic chip, microfluidic test system and microfluidic test method | 
| US20210178391A1 (en)* | 2018-07-09 | 2021-06-17 | Presens Precision Sensing Gmbh | System for Analysis of a Fluid Sample | 
| CN113324114A (en)* | 2020-02-29 | 2021-08-31 | 潍坊嘉腾液压技术有限公司 | Pipe joint assembly for fluid multi-parameter measurement | 
| CN114018303A (en)* | 2021-11-04 | 2022-02-08 | 天津工业大学 | Novel fiber Bragg grating probe for in-situ monitoring of hydraulic shear force | 
| Publication number | Priority date | Publication date | Assignee | Title | 
|---|---|---|---|---|
| CN101995485A (en)* | 2010-11-03 | 2011-03-30 | 山东大学 | Target fiber grating rheometer | 
| CN103512593A (en)* | 2012-06-20 | 2014-01-15 | 山东金煜电子科技有限公司 | Embedded fiber grating sensor and manufacturing method | 
| US20210178391A1 (en)* | 2018-07-09 | 2021-06-17 | Presens Precision Sensing Gmbh | System for Analysis of a Fluid Sample | 
| CN110836853A (en)* | 2019-12-03 | 2020-02-25 | 河南渡盈光电科技有限公司 | Microfluidic chip, microfluidic test system and microfluidic test method | 
| CN113324114A (en)* | 2020-02-29 | 2021-08-31 | 潍坊嘉腾液压技术有限公司 | Pipe joint assembly for fluid multi-parameter measurement | 
| CN114018303A (en)* | 2021-11-04 | 2022-02-08 | 天津工业大学 | Novel fiber Bragg grating probe for in-situ monitoring of hydraulic shear force | 
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| RJ01 | Rejection of invention patent application after publication | Application publication date:20220524 |