CN114195075A - Racking machine for producing cell freezing solution - Google Patents

Abstract

The invention discloses a racking machine for producing cell frozen stock solution, which is used for racking the cell frozen stock solution into a bottle body and comprises: a storage chamber having an opening; a refrigeration device disposed within the storage compartment; the conveying belt is provided with at least one clamp for clamping the bottle body, and one end of the conveying belt penetrates into the storage chamber through the opening; a dispensing apparatus disposed within the storage chamber; at least one closure assembly, the closure assembly comprising: the door is provided with a notch for the conveying belt to pass through; and the rotating unit is used for driving the door to rotate so as to close or open the opening. Utilize the rotation unit to drive the door and rotate, in time seal the opening, guarantee to carry out the partial shipment at low temperature, avoid the cell frozen stock liquid to damage, it is effectual.

Description

Racking machine for producing cell freezing solution

Technical Field

The invention relates to the technical field of cell production, in particular to a racking machine for producing cell freezing solution.

Background

Cell cryopreservation is a technique for placing cells in a low-temperature environment, reducing cell metabolism and facilitating long-term storage. Cell cryopreservation is one of the main methods for cell preservation. The cells are stored in liquid nitrogen at the temperature of-196 ℃ by using a freezing technology at a low temperature, so that the cells can be temporarily separated from a growth state and the cell characteristics can be stored, and the cells are recovered for experiments when needed. Moreover, a certain amount of cells are preserved appropriately, so that the cells can be prevented from being lost due to contamination or other accidents of the cultured cells, and the function of preserving the cells is achieved. In addition, some cells may be purchased, hosted, exchanged, and shipped in their cryopreserved form. When cells are frozen, a protective agent, namely glycerol or dimethyl sulfoxide (DMSO) with the final concentration of 5% to 15% is added into a culture medium, so that the freezing point of the solution can be lowered, and in addition, water in the cells can permeate out under the condition of slow freezing, the formation of ice crystals is reduced, and thus, the cells are prevented from being damaged. The method of 'slow freezing and fast melting' can better ensure the survival of cells. The standard freezing speed is from-1 to-2 ℃/min, the speed can be increased when the temperature is lower than-25 ℃, and the liquid nitrogen can be directly put into the liquid nitrogen after the temperature is lower than-80 ℃. When in use, the frozen cell stock solution needs to be subpackaged.

In chinese patent application No.: CN20202022759.0 discloses a device for cell dispersion and quantitative cell sap subpackaging, wherein a barrel cover is fixed on the top of a cell dispersion barrel, an air respirator, a liquid inlet interface and a liquid outlet interface are fixed on the top of the barrel cover, a dispersion pipe is fixed at the bottom end of the liquid inlet interface, an extension pipe is fixed at the bottom end of the liquid outlet interface, and the device also comprises a three-way valve, a quantitative partial shipment peristaltic pump and a circulation peristaltic pump, go out the top of liquid interface, the feed liquor end of ration partial shipment peristaltic pump and the feed liquor end of circulation peristaltic pump are connected with the one end of three-way valve through the connecting pipe respectively, the play liquid end of circulation peristaltic pump is connected with the top of feed liquor interface through the connecting pipe, the play liquid end of ration partial shipment peristaltic pump is connected with the partial shipment ware through the connecting pipe, still have a branch pipe on the connecting pipe between circulation peristaltic pump and the three-way valve, the end connection of branch pipe has a aspirator, still be provided with a valve on the branch pipe. The frozen cell stock solution needs to be subpackaged at low temperature, and the technical scheme is not suitable.

Disclosure of Invention

In order to solve at least one technical problem in the background art, the invention provides a racking machine for producing cell freezing solution.

The invention provides a racking machine for producing cell frozen stock solution, which is used for racking the cell frozen stock solution into a bottle body and comprises:

a storage chamber having an opening;

a refrigeration device disposed within the storage compartment;

the conveying belt is provided with at least one clamp for clamping the bottle body, and one end of the conveying belt penetrates into the storage chamber through the opening;

a dispensing apparatus disposed within the storage chamber;

at least one closure assembly, the closure assembly comprising:

the door is provided with a notch for the conveying belt to pass through;

and the rotating unit is used for driving the door to rotate so as to close or open the opening.

Preferably, the door is the opening, and the door is rotatably connected with the storage chamber; the rotating unit is in transmission connection with the door.

Preferably, the two closure assemblies are symmetrically arranged.

Preferably, the racking apparatus comprises:

a storage tank;

a first connecting pipe;

a tank body communicating with the storage tank through the first connection pipe;

a first valve installed on the first connection pipe;

at least one nozzle in communication with the tank; wherein,

the bottle body can be placed below or separated from the nozzle.

Preferably, the split charging equipment further comprises at least one adjusting unit, the nozzle is communicated with the box body through the adjusting unit, and the adjusting unit can adjust the number of the nozzles, into which the cell freezing solution in the box body enters.

Preferably, the adjusting unit includes:

the top end of the second connecting pipe is communicated with the box body;

the top end of the third connecting pipe is in threaded connection with the bottom end of the second connecting pipe;

a second valve installed on the third connection pipe, wherein,

the nozzle is communicated with the bottom end of the third connecting pipe.

Preferably, a supporting plate is arranged in the storage chamber, and a plurality of plungers are arranged on the supporting plate;

the racking machine also comprises a power mechanism, and the power mechanism is used for placing the plunger on the bottle body.

Preferably, the power mechanism includes:

a bidirectional cylinder, both ends of which are provided with suckers;

the air pump is communicated with the sucker;

and the air cylinder is used for driving the bidirectional air cylinder to lift.

The beneficial effects brought by one aspect of the invention are as follows:

utilize the rotation unit to drive the door and rotate, in time seal the opening, guarantee to carry out the partial shipment at low temperature, avoid the cell frozen stock liquid to damage, it is effectual.

Drawings

FIG. 1 is a schematic view of a bottle disposed below a nozzle according to the present invention;

FIG. 2 is a schematic view of a structure of the bottle body disposed below the plunger according to the present invention;

FIG. 3 is a schematic view of the bottle removing storage compartment according to the present invention;

FIG. 4 is a schematic view of a closed opening according to the present disclosure;

FIG. 5 is a schematic view of an open opening disclosed herein;

FIG. 6 is a side view of a storage compartment, door, etc. of the present disclosure;

FIG. 7 is a front view of the disclosed door;

fig. 8 is a sectional view of the second and third connection pipes disclosed in the present invention.

Detailed Description

It should be noted that, in the case of no conflict, the embodiments and features in the embodiments in the present application may be combined with each other; the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left" and "right", etc., indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the positions or elements referred to must have specific orientations, be constructed in specific orientations, and be operated, and thus are not to be construed as limitations of the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Referring to fig. 1 to 8, the present invention provides a dispenser for producing a cell freezing solution, for dispensing a cell freezing solution into a vial, comprising:

a storage chamber 1 having anopening 101; a refrigeratingdevice 2 arranged in the storage chamber 1; a conveyor belt 3, on which at least one clamp for clamping the bottle body is arranged, one end of the conveyor belt 3 extends into the storage chamber 1 through theopening 101; a dispensing device disposed within the storage chamber 1; at least one closure assembly, the closure assembly comprising:

adoor 4, which is provided with a gap for the conveyor belt 3 to pass through;

and the rotating unit 5 is used for driving thedoor 4 to rotate so as to close or open theopening 101. The rotating unit 5 may be a rotary cylinder.

Therefrigeration equipment 2 is utilized to ensure that the storage chamber 1 is in a low-temperature state, thereby facilitating split charging.

Thedoor 4 is rotated by the rotating unit 5 to open theopening 101.

The bottles P are transferred to the storage chamber 1 by the conveyor 3 and the clamp, and thedoor 4 is rotated in the reverse direction by the rotation unit 5 to close theopening 101. And then, packaging the cell frozen stock solution into the bottle body by using a packaging device. After the dispensing is completed, thedoor 4 is rotated by the rotating unit 5, and theopening 101 is opened. The bottle bodies are driven by the conveyor belt 3 and then moved out of the storage chamber 1.

Utilize rotation unit 5 to drivedoor 4 and rotate, intime seal opening 101, guarantee to carry out the partial shipment at low temperature, avoid the cell frozen stock liquid to damage, it is effectual.

As a further improvement of the above embodiment, in one embodiment, thedoor 4 is at theopening 101, and thedoor 4 is rotatably connected to the storage chamber 1; the rotating unit 5 is in transmission connection with thedoor 4. The rotating unit 5 is used for driving thedoor 4 to rotate, so that theopening 101 is convenient to open and close.

As a further improvement of the above embodiment, in one embodiment, the two closure assemblies are symmetrically arranged. Facilitating closure of theopening 101.

As a further refinement of the above embodiment, in one embodiment the racking apparatus comprises:

a storage tank 6; a first connecting pipe 7; a tank 8 communicating with the storage tank 6 through the first connection pipe 7; a first valve 9 mounted on said first connection pipe 7; at least onenozzle 10 communicating with said tank 8; wherein the bottle body can be placed under or separated from thenozzle 10.

As a further improvement of the above embodiment, in one embodiment, the dispensing apparatus further comprises at least one adjusting unit, thenozzle 10 is communicated with the box body 8 through the adjusting unit, and the adjusting unit can adjust the number of the cell freezing solution in the box body 8 entering thenozzle 10.

As a further refinement of the above embodiment, in one embodiment the adjustment unit comprises:

a second connectingpipe 11, the top end of which is communicated with the box body 8; a third connectingpipe 12, the top end of which is in threaded connection with the bottom end of the second connectingpipe 11; and asecond valve 13 installed on thethird connection pipe 12, wherein thenozzle 10 communicates with a lower end of thethird connection pipe 12.

The present embodiment provides two regulating units, anozzle 10, a first connecting pipe 7. When the size of the bottle body is changed, the volume of the area S of fig. 8, i.e., the volume above thesecond valve 13 of thethird connection pipe 12 and the volume of thesecond connection pipe 11, is changed by rotating thethird connection pipe 12.

After the volume of the S region is adjusted, the volume of the cell freezing solution entering the tank 8 from the storage tank 6 is controlled by the first valve 9 accordingly. This example is 2 times the volume of the S region.

The first valve 9 is opened to allow the cell culture fluid to enter the tank 8 through the first connecting pipe 7. The first valve 9 is then closed. At this time, all of the cell culture medium in the housing 8 enters the S regions of the two regulating units, and the volumes of the S regions in the two regulating units are the same.

When the bottle is placed under thenozzle 10, thesecond valve 13 is opened to allow access to the bottle. After dispensing, thesecond valve 13 is closed. The vials are moved out of the storage chamber 1 through theopening 101 by the conveyor 3. This embodiment can carry out the partial shipment to two bottles once.

This embodiment can be through rotating third connectingpipe 12, the volume size in adjustment S region adapts to not unidimensional bottle P, and adaptability is good.

As a further improvement of the above embodiment, in one embodiment, asupport plate 14 is provided in the storage chamber 1, and a plurality ofplungers 15 are provided on thesupport plate 14; the racking machine further comprises a power mechanism, and the power mechanism is used for placing theplunger 15 on the bottle body P.

As a further improvement of the above embodiment, in one embodiment, the power mechanism includes:

abidirectional cylinder 16, both ends of which are provided withsuckers 19; anair pump 17, communicated with thesuction cup 19; and the air cylinder is used for driving thebidirectional air cylinder 16 to lift.

Referring to fig. 2, thesuction cup 19 is moved above theplunger 15 by thebidirectional cylinder 16, thecylinder 18 is used to drive thebidirectional cylinder 16 to descend, and theplunger 15 is sucked by thesuction cup 19. Thebi-directional cylinder 16 is then lifted by thecylinder 18. Thebidirectional air cylinder 16 drives thesuction cup 19 and theplunger 15 to contract.

When the bottle body P is arranged below theplunger 15, thecylinder 18 is used for driving thebidirectional cylinder 16, the suckingdisc 19 and theplunger 15 to descend, so that theplunger 15 is arranged at the bottle opening of the bottle body P. Thesuction cup 19 releases theplunger 15.

After the above actions are completed, the conveyor 3 continues to drive the bottles to move left, and the bottles are moved out of the storage chamber 1 as shown in fig. 3.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (8)

1. A racking machine for producing cell freezing solution is used for racking cell freezing solution into a bottle body, and is characterized by comprising:

a storage chamber having an opening;

a refrigeration device disposed within the storage compartment;

the conveying belt is provided with at least one clamp for clamping the bottle body, and one end of the conveying belt penetrates into the storage chamber through the opening;

a dispensing apparatus disposed within the storage chamber;

at least one closure assembly, the closure assembly comprising:

the door is provided with a notch for the conveying belt to pass through;

and the rotating unit is used for driving the door to rotate so as to close or open the opening.

2. The racking machine for producing cell freezing solution according to claim 1, wherein said door is said opening, said door being rotatably connected to said storage chamber; the rotating unit is in transmission connection with the door.

3. The racking machine for producing cell freezing solution according to claim 2, wherein said two sealing members are symmetrically arranged.

4. The racking machine for producing cell cryopreservation liquid according to claim 1, wherein said racking apparatus comprises:

a storage tank;

a first connecting pipe;

a tank body communicating with the storage tank through the first connection pipe;

a first valve installed on the first connection pipe;

at least one nozzle in communication with the tank; wherein,

the bottle body can be placed below or separated from the nozzle.

5. The racking machine for producing cell cryopreservation liquid according to claim 4, wherein said racking equipment further comprises at least one adjusting unit, said nozzle is communicated with said box body through said adjusting unit, said adjusting unit can adjust the number of cell cryopreservation liquid entering said nozzle in said box body.

6. The racking machine for producing cell freezing solution according to claim 5, wherein said adjusting unit comprises:

the top end of the second connecting pipe is communicated with the box body;

the top end of the third connecting pipe is in threaded connection with the bottom end of the second connecting pipe;

a second valve installed on the third connection pipe, wherein,

the nozzle is communicated with the bottom end of the third connecting pipe.

7. The racking machine for producing cell freezing solution according to claim 1, wherein a support plate is provided in said storage chamber, and a plurality of plungers are provided on said support plate;

the racking machine also comprises a power mechanism, and the power mechanism is used for placing the plunger on the bottle body.

8. The racking machine for producing cell freezing solution according to claim 7, wherein said power mechanism comprises:

a bidirectional cylinder, both ends of which are provided with suckers;

the air pump is communicated with the sucker;

and the air cylinder is used for driving the bidirectional air cylinder to lift.

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